pycom14g05640

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0007308
Physical Location & Seq
Forward (+)
5477433 .. 5478035
603 bp
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UTR
Exon/CDS
Intron
pycom14g05640.1

Sequence Viewer

Length: 603 bp
ATGCCTGAACTGGTCGAGCATCTCCGTAAATCACGTCTCCTGCTATGTATCTTTGTTGCATTCCTGTTAAATACTCTTGCGACTTCGGATATTCCCTTGGGTTCCAAAATTTCTGTAGCTGATAAAAACATATGGATCTCTCCAAATGGTGATTTTGCATTTGGATTTTTTAACCGTTCAGACCAGCCAAACTATAGTTTCGGGATCCGTCTCATTTCGAAATCATTTCCTCTTGACCAACAACTTTTGGTATGGAGTGCTGAAGATGATCTTATTATCGGTAGCCATTCTTATGCCCAGCTGACCCAGGATGGCGAACTAGTTTTATTCGATTCCTTGAAGGGTGTCACTGCATGGACCAGTAAAACAAGGCAGTTATCAGTTGTTTCTGCTGCTCTTAATGATAATGGAAATCTTGTTCTATTGAATCAAGAGAAACATATTGTTTGGCAAAGTTTTGATACTCCATCTGACATACTTCTTCCTGGACAGAGCTTCTCTACGTTGCAAACACTTCGAGCTGCAAGCAAGAATTGTTTGTCCAGTTACTACACTCTTTTTATGTGTTGTGGCTCCTTGAAACCATTCTGTTGTTTGCCCTAA

Protein Analysis

201

Amino Acids

22.23

Weight (kDa)

5.86

Isoelectric Point (pI)

38.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 84 - 169 1.6e-16 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 143, 199, 212
AcsI RAATTY 1 cut(s) 108
AcuI CTGAAG 1 cut(s) 282
AgsI TTSAA 3 cut(s) 340, 427, 580
AhlI ACTAGT 1 cut(s) 319
AjiI CACGTC 1 cut(s) 35
AjnI CCWGG 2 cut(s) 306, 484
AloI GAACNNNNNNTCC 2 cut(s) 402, 434
AluBI AGCT 4 cut(s) 119, 301, 495, 521
AluI AGCT 4 cut(s) 119, 301, 495, 521
Alw26I GTCTC 2 cut(s) 41, 215
AlwI GGATC 3 cut(s) 143, 199, 212
ApeKI GCWGC 2 cut(s) 392, 521
ApoI RAATTY 1 cut(s) 108
Asp700I GAANNNNTTC 1 cut(s) 584
AspS9I GGNCC 1 cut(s) 357
AsuHPI GGTGA 1 cut(s) 161
AsuII TTCGAA 1 cut(s) 218
AvaII GGWCC 1 cut(s) 357
BamHI GGATCC 1 cut(s) 204
BbvI GCAGC 2 cut(s) 379, 508
BccI CCATC 2 cut(s) 305, 475
BcgI CGANNNNNNTGC 2 cut(s) 497, 531
BciT130I CCWGG 2 cut(s) 308, 486
BcoDI GTCTC 2 cut(s) 41, 215
BcuI ACTAGT 1 cut(s) 319
BfaI CTAG 1 cut(s) 320
BfmI CTRYAG 2 cut(s) 114, 193
BisI GCNGC 2 cut(s) 393, 522
BlsI GCNGC 2 cut(s) 394, 523
Bme1390I CCNGG 2 cut(s) 308, 486
Bme18I GGWCC 1 cut(s) 357
BmgBI CACGTC 1 cut(s) 35
BmgT120I GGNCC 1 cut(s) 357
BmiI GGNNCC 3 cut(s) 103, 206, 574
BmrFI CCNGG 2 cut(s) 308, 486
BmsI GCATC 1 cut(s) 28
Bpu14I TTCGAA 1 cut(s) 218
BsaJI CCNNGG 2 cut(s) 96, 306
Bse1I ACTGG 3 cut(s) 15, 360, 543
BseBI CCWGG 2 cut(s) 308, 486
BseDI CCNNGG 2 cut(s) 96, 306
BseGI GGATG 1 cut(s) 316
BseNI ACTGG 3 cut(s) 15, 360, 543
BseXI GCAGC 2 cut(s) 379, 508
BseYI CCCAGC 1 cut(s) 297
BsmAI GTCTC 2 cut(s) 41, 215
BsmBI CGTCTC 2 cut(s) 41, 215
BsmI GAATGC 1 cut(s) 59
Bsp119I TTCGAA 1 cut(s) 218
Bsp143I GATC 3 cut(s) 135, 204, 268
BspLI GGNNCC 3 cut(s) 103, 206, 574
BspPI GGATC 3 cut(s) 143, 199, 212
BspT104I TTCGAA 1 cut(s) 218
BsrI ACTGG 3 cut(s) 15, 360, 543
BssECI CCNNGG 2 cut(s) 96, 306
BssMI GATC 3 cut(s) 135, 204, 268
BssT1I CCWWGG 1 cut(s) 96
Bst2UI CCWGG 2 cut(s) 308, 486
Bst4CI ACNGT 1 cut(s) 176
BstBI TTCGAA 1 cut(s) 218
BstC8I GCNNGC 1 cut(s) 526
BstF5I GGATG 1 cut(s) 316
BstKTI GATC 3 cut(s) 138, 207, 271
BstMAI GTCTC 2 cut(s) 41, 215
BstMBI GATC 3 cut(s) 135, 204, 268
BstNI CCWGG 2 cut(s) 308, 486
BstSCI CCNGG 2 cut(s) 306, 484
BstSFI CTRYAG 2 cut(s) 114, 193
BstV1I GCAGC 2 cut(s) 379, 508
BstX2I RGATCY 2 cut(s) 135, 204
BstYI RGATCY 2 cut(s) 135, 204
BtrI CACGTC 1 cut(s) 35
BtsCI GGATG 1 cut(s) 316
BtsI GCAGTG 1 cut(s) 348
BtsIMutI CAGTG 1 cut(s) 348
Cac8I GCNNGC 1 cut(s) 526
Cfr13I GGNCC 1 cut(s) 357
CviAII CATG 1 cut(s) 354
CviJI RGCY 7 cut(s) 119, 187, 285, 301, 495, 521, 573
CviKI_1 RGCY 7 cut(s) 119, 187, 285, 301, 495, 521, 573
DpnI GATC 3 cut(s) 137, 206, 270
DpnII GATC 3 cut(s) 135, 204, 268
Eco130I CCWWGG 1 cut(s) 96
Eco47I GGWCC 1 cut(s) 357
Eco57I CTGAAG 1 cut(s) 282
EcoRII CCWGG 2 cut(s) 306, 484
EcoT14I CCWWGG 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 96
Esp3I CGTCTC 2 cut(s) 41, 215
FaeI CATG 1 cut(s) 357
FalI AAGNNNNNCTT 2 cut(s) 255, 287
FatI CATG 1 cut(s) 353
FauNDI CATATG 1 cut(s) 131
Fnu4HI GCNGC 2 cut(s) 393, 522
FokI GGATG 1 cut(s) 323
Fsp4HI GCNGC 2 cut(s) 393, 522
FspBI CTAG 1 cut(s) 320
GluI GCNGC 2 cut(s) 393, 522
GsaI CCCAGC 1 cut(s) 301
Hin1II CATG 1 cut(s) 357
HinfI GANTC 2 cut(s) 332, 427
HphI GGTGA 1 cut(s) 161
Hpy188I TCNGA 3 cut(s) 88, 181, 472
Hpy188III TCNNGA 3 cut(s) 202, 233, 431
HpyAV CCTTC 1 cut(s) 334
HpyCH4III ACNGT 1 cut(s) 176
HpyCH4IV ACGT 2 cut(s) 34, 503
HpyCH4V TGCA 5 cut(s) 59, 158, 353, 508, 524
HpySE526I ACGT 2 cut(s) 34, 503
Hsp92II CATG 1 cut(s) 357
Kzo9I GATC 3 cut(s) 135, 204, 268
LmnI GCTCC 1 cut(s) 578
Lsp1109I GCAGC 2 cut(s) 379, 508
LweI GCATC 1 cut(s) 28
MaeI CTAG 1 cut(s) 320
MaeII ACGT 2 cut(s) 34, 503
MaeIII GTNAC 2 cut(s) 346, 545
MalI GATC 3 cut(s) 137, 206, 270
MboI GATC 3 cut(s) 135, 204, 268
MboII GAAGA 2 cut(s) 275, 473
MflI RGATCY 2 cut(s) 135, 204
MluCI AATT 2 cut(s) 108, 532
MnlI CCTC 1 cut(s) 240
MroXI GAANNNNTTC 1 cut(s) 584
MseI TTAA 3 cut(s) 68, 171, 399
MslI CAYNNNNRTG 1 cut(s) 291
MspA1I CMGCKG 1 cut(s) 301
MspR9I CCNGG 2 cut(s) 308, 486
Mva1269I GAATGC 1 cut(s) 59
MvaI CCWGG 2 cut(s) 308, 486
NdeI CATATG 1 cut(s) 131
NdeII GATC 3 cut(s) 135, 204, 268
NlaIII CATG 1 cut(s) 357
NlaIV GGNNCC 3 cut(s) 103, 206, 574
NmuCI GTSAC 1 cut(s) 346
NspV TTCGAA 1 cut(s) 218
PctI GAATGC 1 cut(s) 59
PdmI GAANNNNTTC 1 cut(s) 584
PfeI GAWTC 2 cut(s) 332, 427
PfoI TCCNGGA 1 cut(s) 484
PkrI GCNGC 2 cut(s) 394, 523
Psp6I CCWGG 2 cut(s) 306, 484
PspFI CCCAGC 1 cut(s) 297
PspGI CCWGG 2 cut(s) 306, 484
PspN4I GGNNCC 3 cut(s) 103, 206, 574
PspPI GGNCC 1 cut(s) 357
PsuI RGATCY 2 cut(s) 135, 204
PvuII CAGCTG 1 cut(s) 301
RseI CAYNNNNRTG 1 cut(s) 291
SaqAI TTAA 3 cut(s) 68, 171, 399
SatI GCNGC 2 cut(s) 393, 522
Sau3AI GATC 3 cut(s) 135, 204, 268
Sau96I GGNCC 1 cut(s) 357
ScrFI CCNGG 2 cut(s) 308, 486
SetI ASST 6 cut(s) 37, 121, 303, 497, 506, 523
SfaNI GCATC 1 cut(s) 28
SfcI CTRYAG 2 cut(s) 114, 193
SfuI TTCGAA 1 cut(s) 218
SinI GGWCC 1 cut(s) 357
SmiMI CAYNNNNRTG 1 cut(s) 291
SpeI ACTAGT 1 cut(s) 319
Sse9I AATT 2 cut(s) 108, 532
SspMI CTAG 1 cut(s) 320
StyD4I CCNGG 2 cut(s) 306, 484
StyI CCWWGG 1 cut(s) 96
TaaI ACNGT 1 cut(s) 176
TaiI ACGT 2 cut(s) 37, 506
TaqI TCGA 4 cut(s) 15, 218, 330, 517
TasI AATT 2 cut(s) 108, 532
TfiI GAWTC 2 cut(s) 332, 427
Tru1I TTAA 3 cut(s) 68, 171, 399
Tru9I TTAA 3 cut(s) 68, 171, 399
TscAI CASTG 1 cut(s) 355
TseFI GTSAC 1 cut(s) 346
TseI GCWGC 2 cut(s) 392, 521
Tsp45I GTSAC 1 cut(s) 346
TspGWI ACGGA 2 cut(s) 14, 197
TspRI CASTG 1 cut(s) 355
VpaK11BI GGWCC 1 cut(s) 357
XapI RAATTY 1 cut(s) 108
XmnI GAANNNNTTC 1 cut(s) 584
XspI CTAG 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.