Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0007308

Physical Location & Seq

Genomic Coordinates Reverse (-)

42909672 .. 42911900

Gene Structure 2229 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000018924

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2229 bp

ATGCTTCAGCAGGAGGGACTTCTCCTCAGATCATCTTTCCTTCTAAGTATCTTTGTTGGGTTTCTGCTACATTCTCTTGCCATTCCAGAGATTCCTTTAGGTTCAAAACTTTCTGTAGTAGACAAAAACATCTGGGCCTCTCCGAATGGTGATTTCGCATTTGGATTTTTTAACCGTACAGATGACCCTAACTATAGTGTTGGGATCCGTTTCAATTCAAAGTCTATTCCACTTGAAAAACAAACTCTGGTGTGGACTGCTGGAGCTAATATCATGGTTGGTGAAAATTCGTATGTCCAGTTGACCCAGGATGGAGAATTAGTAATCATTGATTCTCTGAAGGAAGTCACTGTATGGAGCAGCAGAACAAGCCGATTATCAGTTGTTTCAGCTGCTCTTCATGATAATGGAAACCTTGTCCTGTGGAATGAAGAGAAACATATTGTTTGGCAAAGTTTTAATACCCCCTCTGATACACTTCTTCCTGGACAGAACTTCTCTAGGTCGCAAATGCTTCGAGCTGCAAGCAAGAATACTATATCTGATTATTTGTCCAGTTACTACACTCTTTTCATGAATGCTTATGGTCAGTTGCAACTCAGATGGGAAATTCATGTTGTCTATTGGACAAGTGGAAGTGCTCCTGGTTCAAATCTCAGCGCTTTCCTCACCTCTGATGGAGCCCTACAACTCCGCGACCAAAACTTGAAACCTGTATGGTCAATTTTTGGGGCAGATTACAATGATTCTATAAGTTACCGGTTTCTTAGGCTAGACGTTGATGGTAATCTACGCTTGTACTCATGGCATGAAGCTTCAATGTCATGGAGAGCAGTCTGGCAAGCTGTTGAGAACCAGTGTAATGTCTTTGCAACTTGTGGCCCACATGGCATCTGTTTCTTTACCGAGTCTGGGTCACCTGATTGTAAATGCCCTTTTAATCTTGTGAATGGGTCCATGGAAAAATGCTCGATTCCGTCTCAGCAATGTGAATCTGGTTCCAACTTGTTTAGTTATAAGCATACTCTCCTATATGGAATGTATACTGATGATTTAGTTGTCGGAACTAGTTTACAGCAATGTAGAAGCTTGTGTCTCAAGGACCCACTATGTACAGCTGCAACCTTCTCAAATGATGGAACTGCACACTGCTTAGTGAAGAGAACACAGTATGTTACTGGCTATTCGGACCCCTCACTAAGTTCAATATCTTTTGTGAAGGTGTGTGCAGATCCATTAGCTGTAAATCCCAATCTCTCCATGTCATCCCCTTCAGCACAGAAGTTTTGTTTTCCTTGTCTAATTGGAGCAGCCTCAGTTGCTGTATTTCTTTTATTTCAGTTGGCACTTGGTTTCTGGTTCTACAGAAGAAGGAAAAGAAGAAGGAATTTGGTCAGGAAGAAAGCCAGTTTTGCTTATACCAGTTCCAATTCAAATGACTTGATTGTGTTATCCTTCTCCGAAATAGAGGACTTTACAGAAAAGTTTAAGCATCAGATTGGACGAGAGATGTTCAAAGCTATTCTTCCAAATAAAGAACCAGTTGCAATCAAAGAGATGAATGCGTCCATTGAAGAAAGAAAATACCGGTCTGCAGTTTCAATTATAGGTGGCATTCATCACAAAAACCTTGTGAAGCTGCAGGGTTACTGTTGTGAGTTAAATCACAAGTTTCTGGTCTATGAATATGTCAAAAATGGTTCTGTGGAGAAATATATAGAAGACCATAAACTGTGTAAGAGGCTGACTTGGGGAAAGAGAGTTGATATATGCTTAAGCGTGGCAAGAGCCATTAGTTATCTACACACAAGCTGCAGGAAATGCATCATCCATGGGAATTTGAAATGCGAGGATGTGTTATTGGATGAAAATTTAGGGGCCAAGGTGACTGGATTCGGGCTTGGGACAATCGTCGGGAATGAAGCATGCTCTTCAGCAACGAGAGATGTAGAAGACTTTGGTAGGATGGTGTTGGTTTTGGTGAGTGGGTGTAGAGAAGTTGGGGACCTCTGTGAATGGGCATACAAAGAGTGGTCAGATGGGCATCCGGAGAATGTAGTTGATAGAAGACTAGATGGTATGCATATAACTCACGAGCTCGAGCGTGTTTTAAGAATTGCATTTTGGTGTCTCCAAATTGATGAACGTCAAAGACCTTCAATGAGTGAGGTGGTTATGGTGTTAGAAGGCACATCGAGCGTTGACCCCCCACCTCCCCCTTTTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin PAN_1 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

743

Amino Acids

83.33

Weight (kDa)

6.98

Isoelectric Point (pI)

40.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	82 - 170	5.9e-17	D-mannose binding lectin
S_locus_glycop	PF00954	248 - 311	1.3e-11	S-locus glycoprotein domain
Pkinase	PF00069	510 - 642	3e-16	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	511 - 640	1.5e-20	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007308)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41890
fragaria_vesca	FvH4_6g26380 FvH4_6g26380
malus_domestica	MD12G1068200.v1.1 MD14G1068300.v1.1 MD14G1068400.v1.1 MD14G1068600.v1.1 MD14G1068700.v1.1 MD14G1069000.v1.1
prunus_persica	Prupe.7G065700_v2.0.a1 Prupe.7G065700_v2.0.a1 Prupe.7G065700_v2.0.a1 Prupe.7G065700_v2.0.a1
pyrus_communis	pycom12g06020 pycom14g05640
rosa_chinensis	RchiOBHm_Chr2g0126871
rosa_laevigata	RLG00000018924
rosa_multiflora	Rmu_sc0000007.1_g000026
rosa_roxburghii	Rroxscaffold_2G00116950
rosa_rugosa	Rorug02G0270000
rosa_samantha	Rh2AG322100 Rh2BG330300 Rh2CG310200 Rh2DG349600
rosa_wichuraiana	Rw2G026120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1017
AccI	GTMKAC	2 cut(s)	120, 1043
AccII	CGCG	1 cut(s)	696
AccIII	TCCGGA	1 cut(s)	2047
AciI	CCGC	1 cut(s)	694
AclWI	GGATC	3 cut(s)	199, 212, 1226
AcsI	RAATTY	5 cut(s)	286, 609, 1387, 1837, 1870
AcuI	CTGAAG	3 cut(s)	359, 1257, 1917
AfaI	GTAC	3 cut(s)	178, 800, 1114
AfeI	AGCGCT	1 cut(s)	661
AfiI	CCNNNNNNNGG	1 cut(s)	912
AflII	CTTAAG	1 cut(s)	1774
AgeI	ACCGGT	2 cut(s)	759, 1587
AhlI	ACTAGT	1 cut(s)	1067
AjnI	CCWGG	3 cut(s)	306, 484, 643
AjuI	GAANNNNNNNTTGG	2 cut(s)	2107, 2139
Alw21I	GWGCWC	2 cut(s)	643, 2100
Alw26I	GTCTC	3 cut(s)	984, 1100, 2135
AlwI	GGATC	3 cut(s)	199, 212, 1226
AlwNI	CAGNNNCTG	1 cut(s)	1322
Ama87I	CYCGRG	1 cut(s)	2099
Aor13HI	TCCGGA	1 cut(s)	2047
Aor51HI	AGCGCT	1 cut(s)	661
AoxI	GGCC	3 cut(s)	135, 880, 1878
ApeKI	GCWGC	7 cut(s)	360, 392, 521, 1118, 1310, 1639, 1812
ApoI	RAATTY	5 cut(s)	286, 609, 1387, 1837, 1870
AsiGI	ACCGGT	2 cut(s)	759, 1587
AspLEI	GCGC	1 cut(s)	662
AspS9I	GGNCC	7 cut(s)	135, 881, 954, 1102, 1189, 1878, 2005
AsuHPI	GGTGA	6 cut(s)	161, 293, 661, 909, 1897, 1993
AvaI	CYCGRG	1 cut(s)	2099
AvaII	GGWCC	4 cut(s)	954, 1102, 1189, 2005
BamHI	GGATCC	1 cut(s)	204
BanII	GRGCYC	2 cut(s)	685, 2100
BauI	CACGAG	1 cut(s)	2093
BbsI	GAAGAC	3 cut(s)	1728, 1959, 2074
Bbv12I	GWGCWC	2 cut(s)	643, 2100
BbvI	GCAGC	7 cut(s)	372, 379, 508, 1105, 1322, 1626, 1799
BccI	CCATC	8 cut(s)	305, 597, 671, 776, 1130, 1960, 2033, 2069
BcgI	CGANNNNNNTGC	2 cut(s)	497, 531
BciT130I	CCWGG	3 cut(s)	308, 486, 645
BcoDI	GTCTC	3 cut(s)	984, 1100, 2135
BcuI	ACTAGT	1 cut(s)	1067
BfaI	CTAG	4 cut(s)	501, 773, 1068, 2072
BfmI	CTRYAG	6 cut(s)	114, 193, 1363, 1593, 1640, 1813
BfoI	RGCGCY	1 cut(s)	663
BfrI	CTTAAG	1 cut(s)	1774
BglI	GCCNNNNNGGC	1 cut(s)	888
BisI	GCNGC	7 cut(s)	361, 393, 522, 1119, 1311, 1640, 1813
BlsI	GCNGC	7 cut(s)	362, 394, 523, 1120, 1312, 1641, 1814
Bme1390I	CCNGG	3 cut(s)	308, 486, 645
Bme18I	GGWCC	4 cut(s)	954, 1102, 1189, 2005
BmeT110I	CYCGRG	1 cut(s)	2099
BmgT120I	GGNCC	7 cut(s)	135, 881, 954, 1102, 1189, 1878, 2005
BmiI	GGNNCC	8 cut(s)	206, 682, 955, 1000, 1104, 1191, 1879, 2006
BmrFI	CCNGG	3 cut(s)	308, 486, 645
BmsI	GCATC	4 cut(s)	900, 1501, 1833, 2053
BoxI	GACNNNNGTC	1 cut(s)	1910
BpiI	GAAGAC	3 cut(s)	1728, 1959, 2074
BpmI	CTGGAG	1 cut(s)	282
BpuEI	CTTGAG	1 cut(s)	1082
BsaBI	GATNNNNATC	2 cut(s)	786, 2043
BsaJI	CCNNGG	4 cut(s)	306, 957, 1831, 1881
BsaWI	WCCGGW	3 cut(s)	759, 1587, 2047
BsaXI	ACNNNNNCTCC	2 cut(s)	306, 336
Bsc4I	CCNNNNNNNGG	1 cut(s)	912
Bse118I	RCCGGY	2 cut(s)	759, 1587
Bse1I	ACTGG	8 cut(s)	298, 555, 856, 1183, 1407, 1422, 1541, 1894
Bse3DI	GCAATG	2 cut(s)	992, 1085
Bse8I	GATNNNNATC	2 cut(s)	786, 2043
BseAI	TCCGGA	1 cut(s)	2047
BseBI	CCWGG	3 cut(s)	308, 486, 645
BseDI	CCNNGG	4 cut(s)	306, 957, 1831, 1881
BseGI	GGATG	7 cut(s)	316, 1265, 1827, 1858, 1870, 1971, 2044
BseJI	GATNNNNATC	2 cut(s)	786, 2043
BseLI	CCNNNNNNNGG	1 cut(s)	912
BseMI	GCAATG	2 cut(s)	992, 1085
BseMII	CTCAG	5 cut(s)	40, 613, 670, 995, 1329
BseNI	ACTGG	8 cut(s)	298, 555, 856, 1183, 1407, 1422, 1541, 1894
BseRI	GAGGAG	1 cut(s)	14
BseXI	GCAGC	7 cut(s)	372, 379, 508, 1105, 1322, 1626, 1799
BsgI	GTGCAG	2 cut(s)	1128, 1248
Bsh1236I	CGCG	1 cut(s)	696
BshFI	GGCC	3 cut(s)	137, 882, 1880
BshTI	ACCGGT	2 cut(s)	759, 1587
BsiHKAI	GWGCWC	2 cut(s)	643, 2100
BsiHKCI	CYCGRG	1 cut(s)	2099
BsiSI	CCGG	3 cut(s)	760, 1588, 2048
BslFI	GGGAC	3 cut(s)	30, 1918, 2018
BslI	CCNNNNNNNGG	1 cut(s)	912
BsmAI	GTCTC	3 cut(s)	984, 1100, 2135
BsmBI	CGTCTC	1 cut(s)	984
BsmFI	GGGAC	3 cut(s)	30, 1918, 2018
BsmI	GAATGC	3 cut(s)	583, 1567, 1614
BsnI	GGCC	3 cut(s)	137, 882, 1880
BsoBI	CYCGRG	1 cut(s)	2099
Bsp1286I	GDGCHC	3 cut(s)	643, 685, 2100
Bsp13I	TCCGGA	1 cut(s)	2047
Bsp1407I	TGTACA	1 cut(s)	1112
Bsp143I	GATC	3 cut(s)	29, 204, 1231
Bsp19I	CCATGG	2 cut(s)	957, 1831
BspACI	CCGC	1 cut(s)	694
BspANI	GGCC	3 cut(s)	137, 882, 1880
BspCNI	CTCAG	5 cut(s)	39, 612, 669, 994, 1328
BspEI	TCCGGA	1 cut(s)	2047
BspFNI	CGCG	1 cut(s)	696
BspHI	TCATGA	2 cut(s)	400, 573
BspLI	GGNNCC	8 cut(s)	206, 682, 955, 1000, 1104, 1191, 1879, 2006
BspMAI	CTGCAG	3 cut(s)	1597, 1644, 1817
BspPI	GGATC	3 cut(s)	199, 212, 1226
BspQI	GCTCTTC	2 cut(s)	402, 1936
BspTI	CTTAAG	1 cut(s)	1774
BsrDI	GCAATG	2 cut(s)	992, 1085
BsrFI	RCCGGY	2 cut(s)	759, 1587
BsrGI	TGTACA	1 cut(s)	1112
BsrI	ACTGG	8 cut(s)	298, 555, 856, 1183, 1407, 1422, 1541, 1894
BssAI	RCCGGY	2 cut(s)	759, 1587
BssECI	CCNNGG	4 cut(s)	306, 957, 1831, 1881
BssMI	GATC	3 cut(s)	29, 204, 1231
BssNAI	GTATAC	1 cut(s)	1044
BssSI	CACGAG	1 cut(s)	2093
BssT1I	CCWWGG	3 cut(s)	957, 1831, 1881
Bst1107I	GTATAC	1 cut(s)	1044
Bst2BI	CACGAG	1 cut(s)	2093
Bst2UI	CCWGG	3 cut(s)	308, 486, 645
Bst4CI	ACNGT	5 cut(s)	176, 352, 1170, 1652, 1734
Bst6I	CTCTTC	4 cut(s)	402, 426, 1154, 1936
BstAFI	CTTAAG	1 cut(s)	1774
BstAPI	GCANNNNNTGC	1 cut(s)	1821
BstAUI	TGTACA	1 cut(s)	1112
BstC8I	GCNNGC	3 cut(s)	526, 843, 1927
BstDEI	CTNAG	9 cut(s)	26, 44, 599, 656, 767, 981, 1153, 1199, 1315
BstDSI	CCRYGG	2 cut(s)	957, 1831
BstEII	GGTNACC	1 cut(s)	915
BstF5I	GGATG	7 cut(s)	316, 1265, 1827, 1858, 1870, 1971, 2044
BstFNI	CGCG	1 cut(s)	696
BstH2I	RGCGCY	1 cut(s)	663
BstHHI	GCGC	1 cut(s)	662
BstKTI	GATC	3 cut(s)	32, 207, 1234
BstMAI	GTCTC	3 cut(s)	984, 1100, 2135
BstMBI	GATC	3 cut(s)	29, 204, 1231
BstMWI	GCNNNNNNNGC	6 cut(s)	369, 888, 1319, 1412, 1821, 2196
BstNI	CCWGG	3 cut(s)	308, 486, 645
BstNSI	RCATGY	1 cut(s)	1929
BstPAI	GACNNNNGTC	1 cut(s)	1910
BstPI	GGTNACC	1 cut(s)	915
BstSCI	CCNGG	3 cut(s)	306, 484, 643
BstSFI	CTRYAG	6 cut(s)	114, 193, 1363, 1593, 1640, 1813
BstUI	CGCG	1 cut(s)	696
BstV1I	GCAGC	7 cut(s)	372, 379, 508, 1105, 1322, 1626, 1799
BstV2I	GAAGAC	3 cut(s)	1728, 1959, 2074
BstX2I	RGATCY	2 cut(s)	204, 1231
BstYI	RGATCY	2 cut(s)	204, 1231
BstZ17I	GTATAC	1 cut(s)	1044
BsuRI	GGCC	3 cut(s)	137, 882, 1880
BtgI	CCRYGG	2 cut(s)	957, 1831
BtsCI	GGATG	7 cut(s)	316, 1265, 1827, 1858, 1870, 1971, 2044
BtsI	GCAGTG	1 cut(s)	1147
BtsIMutI	CAGTG	3 cut(s)	348, 863, 1147
Cac8I	GCNNGC	3 cut(s)	526, 843, 1927
CaiI	CAGNNNCTG	1 cut(s)	1322
CciI	TCATGA	2 cut(s)	400, 573
CfoI	GCGC	1 cut(s)	662
Cfr10I	RCCGGY	2 cut(s)	759, 1587
Cfr13I	GGNCC	7 cut(s)	135, 881, 954, 1102, 1189, 1878, 2005
CseI	GACGC	1 cut(s)	1554
Csp6I	GTAC	3 cut(s)	177, 799, 1113
CspAI	ACCGGT	2 cut(s)	759, 1587
CviQI	GTAC	3 cut(s)	177, 799, 1113
DdeI	CTNAG	9 cut(s)	26, 44, 599, 656, 767, 981, 1153, 1199, 1315
DpnI	GATC	3 cut(s)	31, 206, 1233
DpnII	GATC	3 cut(s)	29, 204, 1231
Eam1104I	CTCTTC	4 cut(s)	402, 426, 1154, 1936
EarI	CTCTTC	4 cut(s)	402, 426, 1154, 1936
Ecl136II	GAGCTC	1 cut(s)	2098
Eco130I	CCWWGG	3 cut(s)	957, 1831, 1881
Eco24I	GRGCYC	2 cut(s)	685, 2100
Eco47I	GGWCC	4 cut(s)	954, 1102, 1189, 2005
Eco47III	AGCGCT	1 cut(s)	661
Eco53kI	GAGCTC	1 cut(s)	2098
Eco57I	CTGAAG	3 cut(s)	359, 1257, 1917
Eco88I	CYCGRG	1 cut(s)	2099
Eco91I	GGTNACC	1 cut(s)	915
EcoICRI	GAGCTC	1 cut(s)	2098
EcoO109I	RGGNCCY	2 cut(s)	1102, 2005
EcoO65I	GGTNACC	1 cut(s)	915
EcoRII	CCWGG	3 cut(s)	306, 484, 643
EcoT14I	CCWWGG	3 cut(s)	957, 1831, 1881
EcoT22I	ATGCAT	2 cut(s)	1826, 2085
EcoT38I	GRGCYC	2 cut(s)	685, 2100
ErhI	CCWWGG	3 cut(s)	957, 1831, 1881
Esp3I	CGTCTC	1 cut(s)	984
FalI	AAGNNNNNCTT	2 cut(s)	1509, 1541
FaqI	GGGAC	3 cut(s)	30, 1918, 2018
FblI	GTMKAC	2 cut(s)	120, 1043
Fnu4HI	GCNGC	7 cut(s)	361, 393, 522, 1119, 1311, 1640, 1813
FokI	GGATG	7 cut(s)	323, 1252, 1814, 1865, 1877, 1978, 2031
FriOI	GRGCYC	2 cut(s)	685, 2100
Fsp4HI	GCNGC	7 cut(s)	361, 393, 522, 1119, 1311, 1640, 1813
FspBI	CTAG	4 cut(s)	501, 773, 1068, 2072
GlaI	GCGC	1 cut(s)	661
GluI	GCNGC	7 cut(s)	361, 393, 522, 1119, 1311, 1640, 1813
GsuI	CTGGAG	1 cut(s)	282
HaeII	RGCGCY	1 cut(s)	663
HaeIII	GGCC	3 cut(s)	137, 882, 1880
HapII	CCGG	3 cut(s)	760, 1588, 2048
HgaI	GACGC	1 cut(s)	1554
HhaI	GCGC	1 cut(s)	662
Hin6I	GCGC	1 cut(s)	660
HinP1I	GCGC	1 cut(s)	660
HincII	GTYRAC	2 cut(s)	303, 2203
HindII	GTYRAC	2 cut(s)	303, 2203
HindIII	AAGCTT	2 cut(s)	813, 1087
HinfI	GANTC	7 cut(s)	91, 332, 746, 908, 973, 992, 1893
HpaII	CCGG	3 cut(s)	760, 1588, 2048
HphI	GGTGA	6 cut(s)	161, 293, 661, 909, 1897, 1993
Hpy166II	GTNNAC	6 cut(s)	121, 255, 303, 1044, 1073, 2203
Hpy188III	TCNNGA	7 cut(s)	86, 401, 574, 1396, 1915, 2048, 2093
Hpy8I	GTNNAC	6 cut(s)	121, 255, 303, 1044, 1073, 2203
Hpy99I	CGWCG	1 cut(s)	1916
HpyCH4III	ACNGT	5 cut(s)	176, 352, 1170, 1652, 1734
HpyCH4IV	ACGT	2 cut(s)	777, 2146
HpyF10VI	GCNNNNNNNGC	6 cut(s)	369, 888, 1319, 1412, 1821, 2196
HpyF3I	CTNAG	9 cut(s)	26, 44, 599, 656, 767, 981, 1153, 1199, 1315
HpySE526I	ACGT	2 cut(s)	777, 2146
HspAI	GCGC	1 cut(s)	660
Kpn2I	TCCGGA	1 cut(s)	2047
Kzo9I	GATC	3 cut(s)	29, 204, 1231
LguI	GCTCTTC	2 cut(s)	402, 1936
LmnI	GCTCC	5 cut(s)	263, 357, 646, 680, 1307
Lsp1109I	GCAGC	7 cut(s)	372, 379, 508, 1105, 1322, 1626, 1799
LweI	GCATC	4 cut(s)	900, 1501, 1833, 2053
MaeI	CTAG	4 cut(s)	501, 773, 1068, 2072
MaeII	ACGT	2 cut(s)	777, 2146
MaeIII	GTNAC	7 cut(s)	346, 557, 755, 915, 1174, 1646, 1885
MalI	GATC	3 cut(s)	31, 206, 1233
MboI	GATC	3 cut(s)	29, 204, 1231
MflI	RGATCY	2 cut(s)	204, 1231
MhlI	GDGCHC	3 cut(s)	643, 685, 2100
MlyI	GAGTC	1 cut(s)	917
MmeI	TCCRAC	2 cut(s)	1026, 1042
Mph1103I	ATGCAT	2 cut(s)	1826, 2085
MroI	TCCGGA	1 cut(s)	2047
MseI	TTAA	7 cut(s)	171, 459, 939, 1488, 1661, 1775, 2111
MslI	CAYNNNNRTG	2 cut(s)	405, 2125
MspA1I	CMGCKG	2 cut(s)	392, 1118
MspCI	CTTAAG	1 cut(s)	1774
MspI	CCGG	3 cut(s)	760, 1588, 2048
MspR9I	CCNGG	3 cut(s)	308, 486, 645
Mva1269I	GAATGC	3 cut(s)	583, 1567, 1614
MvaI	CCWGG	3 cut(s)	308, 486, 645
MvnI	CGCG	1 cut(s)	696
MwoI	GCNNNNNNNGC	6 cut(s)	369, 888, 1319, 1412, 1821, 2196
NcoI	CCATGG	2 cut(s)	957, 1831
NdeII	GATC	3 cut(s)	29, 204, 1231
NlaIV	GGNNCC	8 cut(s)	206, 682, 955, 1000, 1104, 1191, 1879, 2006
NmuCI	GTSAC	3 cut(s)	346, 915, 1885
NsiI	ATGCAT	2 cut(s)	1826, 2085
NspI	RCATGY	1 cut(s)	1929
PaeI	GCATGC	1 cut(s)	1929
PaeR7I	CTCGAG	1 cut(s)	2099
PagI	TCATGA	2 cut(s)	400, 573
PciSI	GCTCTTC	2 cut(s)	402, 1936
PctI	GAATGC	3 cut(s)	583, 1567, 1614
PfeI	GAWTC	6 cut(s)	91, 332, 746, 973, 992, 1893
PfoI	TCCNGGA	1 cut(s)	484
PinAI	ACCGGT	2 cut(s)	759, 1587
PkrI	GCNGC	7 cut(s)	362, 394, 523, 1120, 1312, 1641, 1814
PleI	GAGTC	1 cut(s)	916
PpsI	GAGTC	1 cut(s)	916
PpuMI	RGGWCCY	2 cut(s)	1102, 2005
PshAI	GACNNNNGTC	1 cut(s)	1910
PsiI	TTATAA	1 cut(s)	1017
Psp124BI	GAGCTC	1 cut(s)	2100
Psp5II	RGGWCCY	2 cut(s)	1102, 2005
Psp6I	CCWGG	3 cut(s)	306, 484, 643
PspEI	GGTNACC	1 cut(s)	915
PspGI	CCWGG	3 cut(s)	306, 484, 643
PspN4I	GGNNCC	8 cut(s)	206, 682, 955, 1000, 1104, 1191, 1879, 2006
PspPI	GGNCC	7 cut(s)	135, 881, 954, 1102, 1189, 1878, 2005
PspPPI	RGGWCCY	2 cut(s)	1102, 2005
PspXI	VCTCGAGB	1 cut(s)	2099
PstI	CTGCAG	3 cut(s)	1597, 1644, 1817
PstNI	CAGNNNCTG	1 cut(s)	1322
PsuI	RGATCY	2 cut(s)	204, 1231
PvuII	CAGCTG	2 cut(s)	392, 1118
RsaI	GTAC	3 cut(s)	178, 800, 1114
RsaNI	GTAC	3 cut(s)	177, 799, 1113
RseI	CAYNNNNRTG	2 cut(s)	405, 2125
SacI	GAGCTC	1 cut(s)	2100
SapI	GCTCTTC	2 cut(s)	402, 1936
SaqAI	TTAA	7 cut(s)	171, 459, 939, 1488, 1661, 1775, 2111
SatI	GCNGC	7 cut(s)	361, 393, 522, 1119, 1311, 1640, 1813
Sau3AI	GATC	3 cut(s)	29, 204, 1231
Sau96I	GGNCC	7 cut(s)	135, 881, 954, 1102, 1189, 1878, 2005
SchI	GAGTC	1 cut(s)	917
ScrFI	CCNGG	3 cut(s)	308, 486, 645
SduI	GDGCHC	3 cut(s)	643, 685, 2100
SfaNI	GCATC	4 cut(s)	900, 1501, 1833, 2053
SfcI	CTRYAG	6 cut(s)	114, 193, 1363, 1593, 1640, 1813
Sfr274I	CTCGAG	1 cut(s)	2099
SinI	GGWCC	4 cut(s)	954, 1102, 1189, 2005
SlaI	CTCGAG	1 cut(s)	2099
SmiMI	CAYNNNNRTG	2 cut(s)	405, 2125
SmlI	CTYRAG	3 cut(s)	1097, 1774, 2099
SmoI	CTYRAG	3 cut(s)	1097, 1774, 2099
SpeI	ACTAGT	1 cut(s)	1067
SphI	GCATGC	1 cut(s)	1929
SsiI	CCGC	1 cut(s)	694
SspMI	CTAG	4 cut(s)	501, 773, 1068, 2072
SstI	GAGCTC	1 cut(s)	2100
StyD4I	CCNGG	3 cut(s)	306, 484, 643
StyI	CCWWGG	3 cut(s)	957, 1831, 1881
TaaI	ACNGT	5 cut(s)	176, 352, 1170, 1652, 1734
TaiI	ACGT	2 cut(s)	780, 2149
TaqI	TCGA	4 cut(s)	517, 971, 2100, 2195
TatI	WGTACW	2 cut(s)	798, 1112
TfiI	GAWTC	6 cut(s)	91, 332, 746, 973, 992, 1893
Tru1I	TTAA	7 cut(s)	171, 459, 939, 1488, 1661, 1775, 2111
Tru9I	TTAA	7 cut(s)	171, 459, 939, 1488, 1661, 1775, 2111
TscAI	CASTG	3 cut(s)	355, 863, 1154
TseFI	GTSAC	3 cut(s)	346, 915, 1885
TseI	GCWGC	7 cut(s)	360, 392, 521, 1118, 1310, 1639, 1812
Tsp45I	GTSAC	3 cut(s)	346, 915, 1885
TspGWI	ACGGA	2 cut(s)	197, 966
TspRI	CASTG	3 cut(s)	355, 863, 1154
Vha464I	CTTAAG	1 cut(s)	1774
VpaK11BI	GGWCC	4 cut(s)	954, 1102, 1189, 2005
XapI	RAATTY	5 cut(s)	286, 609, 1387, 1837, 1870
XceI	RCATGY	1 cut(s)	1929
XhoI	CTCGAG	1 cut(s)	2099
XmiI	GTMKAC	2 cut(s)	120, 1043
XspI	CTAG	4 cut(s)	501, 773, 1068, 2072
Zsp2I	ATGCAT	2 cut(s)	1826, 2085

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.