Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0007308

Physical Location & Seq

Genomic Coordinates Forward (+)

6041802 .. 6044072

Gene Structure 2271 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g06020.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2271 bp

ATGCCTGAACTGGTTGAACATCTCCCTAAATCGTATCTCCTGCTGTGCATCTTTGTTGTGTTCCTGTTACATACTCTTTCCGGTGCAGAGATTCCCTTAGGTTCAAAACTTTCTGTGGCCGATGAAAACATATGGATCTCTCCAAATGGTGATTTTGCATTTGGATTTTTTAACTGTTCAGACCAGCCAAACTATAGTTTTGGGATCCGTTTCAATTCAAAATCCATTCCGCTTGACCAACAACTTTTAGTATGGAGTGCTGCAGATGGTCTTATTCTTGGTAACGATTCTTATGCCCAGCTGACCCAGGATGGCGAACTTGTTTTATTTGATTCCTTGAAGGGTGTCACTGCATGGACCAGTAAAACAAGGCAATTATCAGTTGTTTCTGCTGCTCTTAATGACAACGGAAATCTTGTTCTATTGAATCAAGAGAAACATATTGTTTGGCAAAGTTTTGATTCTCCATCTGACATACTTCTTCCTGTACAGAACTTCTCTACATTTCAAACACTTCGAGCTGCTAGCAGGAATAGTGTGTCCAGTTACTACACTCTTTTCATGAGTGCTTCTAGTCAGTTGCAACTTCGATGGGAAAGTCACGTCACCTACTGGACAAGTGGAAGCCCTTCTAGTTCAAACCTCAGTGCTTTCATCACTTCTGATGGAGCCCTACTACTCCGTGATCAAAACTTGAAACCTCTGTGGTCACTATACGGTCAAGATCACAATGACTCTGTAAATTACAGGTTTCTTAGGCTAGATGTTGATGGTAATCTCCGGTTATACTCATGGGTAGAAGAATCAAAGTCATGGAGATCAGTCTGGCAAGCAGTTGAGAACCAGTGTGATGTCTTTGCAACCTGTGGCCAACGTGGCATCTGTGTTTTCAATGAATCTGGGTTCCCTGATTGCGAATGTCCATTTAAGCTGCAGACAAATGAGTCCAATTCCAGATGTTTTATTCCAAATCATCGGTGTGCTTCTGGTTCCAACATGCAACATTATAAGCATACTTCCCTGCATGGAATGTACCCACCAACTGATGATGTAGTTGCCAAAGTTAGTCTAGAGAAATGCAAAAGCTTGTGTCAGAATGACCTGACTTGTACAGCTGCAACCTTTACAAATGATGGAACTGCACGATGCTTAATTAAGAGAACACCGTATGTTACTGGCTATTCAGACCCCTCACTGAGTTCAGTATCTTACGTGAAGATGTGTGCAGATCCATTAGCTGTAAATCCCAATCTCTCGTTGCCTTCCCCTCCTCCACTCAATCGGTCTCATAAGTTTTGTTTCCCTTGCTTAATCGGAGCAGCCTCAGGAGTGTTCGTTGTATTTGTTTTAGTTCAATTGGCACTTGGTTTCTGGTTCTACAGAAGAAGAAATTTGGATAGGAAGAAAGCCGCTTTTGCTCATACCAGCCCAAACTCGAATGGTTTGATAGTGTTATCCTTCTCTGAAATAGAGGAGCTTACAGAGAACTTCAAATATCAAATTGGGCCAAAGATGTTCAAAGGTGTTCTTCCAAATAGAAAACAAGTTGCAATCAAAGATGTGGATGCAAGCGTAGAAGAAAGAAAATACCGGTGTGTAGTTGCAAAGATAGGAAACATTCATCACAAAAGCCTTGTGAAGCTGCAGGGCTACTGTTGTGAGTTAGATCACAGATTTCTAGTCTATGAATATGTCAAGAATGGTTCTCTGGAAAAGTATATAGAAGATGTTAAACTCTGTAAGAAGCTGACTTGGGGGAAGAGATTTGATATATGCTTAAGTGTCGCAAGGGCCATTTGTTATCTGCACACAAGCTGTAGGGAATTTATGAGCCATGGGAATTTGAAATGCGAGAATGTGGTACTGGATGAAAATTTAGAGGCCAAGGTGACTGAATTTGGACTTGGGAACATAGTCAGCAAGGCATCATGCTCTTCATGCTCTTCTGCAGAGAGAGATGTGGAGGACTTTGGTAAGATGGTGTTAGTATTAGTAAGTGGGTGTCGAGTTGGGGACCTTTGTGAGTGGGCATACGAAGAGTGGCTGCAAGGGCATCCAGAGAATGTGGTAGACAGGAAAATAATTGGTGGGTTTATTGCGCAAGAGCTGGAACGTGCTTTAAGAATTGCATTTTGGTGTGTCCAAACTGATGAACGTAGAAGACCTCCAATGAGAGAGGTGGTTAAGGTCTTGGAGGGCACCTTGAGCGTTGATCCACCACCACCCCCTTTTGCTTGTCAAGGGCCACTTGATGATGAAGAGGAGCCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin PAN_1 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

757

Amino Acids

84.88

Weight (kDa)

6.14

Isoelectric Point (pI)

41.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	94 - 168	3.8e-14	D-mannose binding lectin
S_locus_glycop	PF00954	244 - 307	4.3e-12	S-locus glycoprotein domain
PAN_1	PF00024	332 - 404	4.3e-06	PAN domain
PK_Tyr_Ser-Thr	PF07714	512 - 643	9.8e-18	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	512 - 643	1.5e-14	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0007308)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41890
fragaria_vesca	FvH4_6g26380 FvH4_6g26380
malus_domestica	MD12G1068200.v1.1 MD14G1068300.v1.1 MD14G1068400.v1.1 MD14G1068600.v1.1 MD14G1068700.v1.1 MD14G1069000.v1.1
prunus_persica	Prupe.7G065700_v2.0.a1 Prupe.7G065700_v2.0.a1 Prupe.7G065700_v2.0.a1 Prupe.7G065700_v2.0.a1
pyrus_communis	pycom12g06020 pycom14g05640
rosa_chinensis	RchiOBHm_Chr2g0126871
rosa_laevigata	RLG00000018924
rosa_multiflora	Rmu_sc0000007.1_g000026
rosa_roxburghii	Rroxscaffold_2G00116950
rosa_rugosa	Rorug02G0270000
rosa_samantha	Rh2AG322100 Rh2BG330300 Rh2CG310200 Rh2DG349600
rosa_wichuraiana	Rw2G026120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1008
AasI	GACNNNNNNGTC	1 cut(s)	943
Acc16I	TGCGCA	1 cut(s)	2100
AccB1I	GGYRCC	1 cut(s)	2198
AccI	GTMKAC	1 cut(s)	2070
AciI	CCGC	2 cut(s)	230, 1410
AclWI	GGATC	5 cut(s)	143, 199, 212, 1223, 2207
AcoI	YGGCCR	2 cut(s)	117, 868
AcsI	RAATTY	5 cut(s)	1390, 1823, 1840, 1873, 1895
AfaI	GTAC	4 cut(s)	489, 1034, 1111, 1863
AflII	CTTAAG	1 cut(s)	1777
AgeI	ACCGGT	1 cut(s)	1590
AjiI	CACGTC	1 cut(s)	604
AjnI	CCWGG	1 cut(s)	306
AjuI	GAANNNNNNNTTGG	4 cut(s)	1887, 1919, 2116, 2148
AloI	GAACNNNNNNTCC	2 cut(s)	402, 434
Alw26I	GTCTC	1 cut(s)	1290
AlwI	GGATC	5 cut(s)	143, 199, 212, 1223, 2207
AoxI	GGCC	6 cut(s)	117, 868, 1505, 1791, 1881, 2243
ApeKI	GCWGC	8 cut(s)	260, 392, 521, 931, 1115, 1319, 1642, 2044
ApoI	RAATTY	5 cut(s)	1390, 1823, 1840, 1873, 1895
AsiGI	ACCGGT	1 cut(s)	1590
Asp700I	GAANNNNTTC	1 cut(s)	628
AspLEI	GCGC	1 cut(s)	2101
AspS9I	GGNCC	5 cut(s)	357, 1505, 1791, 2014, 2243
AsuHPI	GGTGA	3 cut(s)	161, 598, 1900
AsuNHI	GCTAGC	1 cut(s)	524
AvaII	GGWCC	2 cut(s)	357, 2014
AxyI	CCTNAGG	2 cut(s)	97, 1324
BaeGI	GKGCMC	1 cut(s)	2201
BalI	TGGCCA	1 cut(s)	870
BamHI	GGATCC	1 cut(s)	204
BanI	GGYRCC	1 cut(s)	2198
BanII	GRGCYC	1 cut(s)	673
BbsI	GAAGAC	1 cut(s)	2167
BbvI	GCAGC	8 cut(s)	247, 379, 508, 918, 1102, 1331, 1629, 2031
BccI	CCATC	8 cut(s)	260, 305, 475, 585, 659, 764, 1127, 1972
BceAI	ACGGC	1 cut(s)	2251
BcgI	CGANNNNNNTGC	2 cut(s)	275, 309
BciT130I	CCWGG	1 cut(s)	308
BclI	TGATCA	1 cut(s)	685
BcoDI	GTCTC	1 cut(s)	1290
BfaI	CTAG	6 cut(s)	525, 573, 633, 761, 1070, 1679
BfmI	CTRYAG	7 cut(s)	193, 261, 932, 1378, 1643, 1816, 1947
BfrI	CTTAAG	1 cut(s)	1777
BglI	GCCNNNNNGGC	1 cut(s)	876
BisI	GCNGC	9 cut(s)	261, 393, 522, 932, 1116, 1320, 1410, 1643, 2045
BlsI	GCNGC	9 cut(s)	262, 394, 523, 933, 1117, 1321, 1411, 1644, 2046
Bme1390I	CCNGG	1 cut(s)	308
Bme18I	GGWCC	2 cut(s)	357, 2014
BmgBI	CACGTC	1 cut(s)	604
BmgT120I	GGNCC	5 cut(s)	357, 1505, 1791, 2014, 2243
BmiI	GGNNCC	7 cut(s)	206, 670, 905, 991, 2015, 2200, 2265
BmrFI	CCNGG	1 cut(s)	308
BmsI	GCATC	6 cut(s)	57, 888, 1136, 1555, 1934, 2062
BmtI	GCTAGC	1 cut(s)	528
BpiI	GAAGAC	1 cut(s)	2167
BpuEI	CTTGAG	1 cut(s)	2224
BsaAI	YACGTR	1 cut(s)	1213
BsaBI	GATNNNNATC	1 cut(s)	774
BsaI	GGTCTC	1 cut(s)	1290
BsaJI	CCNNGG	3 cut(s)	306, 1834, 1884
BsaWI	WCCGGW	3 cut(s)	80, 780, 1590
BsaXI	ACNNNNNCTCC	2 cut(s)	1320, 1350
Bse118I	RCCGGY	1 cut(s)	1590
Bse1I	ACTGG	7 cut(s)	15, 360, 543, 617, 844, 1180, 1869
Bse21I	CCTNAGG	2 cut(s)	97, 1324
Bse8I	GATNNNNATC	1 cut(s)	774
BseBI	CCWGG	1 cut(s)	308
BseDI	CCNNGG	3 cut(s)	306, 1834, 1884
BseGI	GGATG	4 cut(s)	316, 1570, 1873, 2053
BseJI	GATNNNNATC	1 cut(s)	774
BseMII	CTCAG	3 cut(s)	658, 1187, 1338
BseNI	ACTGG	7 cut(s)	15, 360, 543, 617, 844, 1180, 1869
BseRI	GAGGAG	2 cut(s)	1260, 1487
BseSI	GKGCMC	1 cut(s)	2201
BseXI	GCAGC	8 cut(s)	247, 379, 508, 918, 1102, 1331, 1629, 2031
BseYI	CCCAGC	1 cut(s)	297
BsgI	GTGCAG	4 cut(s)	105, 1125, 1245, 1790
BshFI	GGCC	6 cut(s)	119, 870, 1507, 1793, 1883, 2245
BshNI	GGYRCC	1 cut(s)	2198
BshTI	ACCGGT	1 cut(s)	1590
BsiSI	CCGG	3 cut(s)	81, 781, 1591
BslFI	GGGAC	1 cut(s)	2027
BsmAI	GTCTC	1 cut(s)	1290
BsmFI	GGGAC	1 cut(s)	2027
BsnI	GGCC	6 cut(s)	119, 870, 1507, 1793, 1883, 2245
Bso31I	GGTCTC	1 cut(s)	1290
Bsp1286I	GDGCHC	2 cut(s)	673, 2201
Bsp1407I	TGTACA	2 cut(s)	487, 1109
Bsp143I	GATC	8 cut(s)	135, 204, 685, 724, 818, 1228, 1666, 2212
Bsp19I	CCATGG	1 cut(s)	1834
BspACI	CCGC	2 cut(s)	230, 1410
BspANI	GGCC	6 cut(s)	119, 870, 1507, 1793, 1883, 2245
BspCNI	CTCAG	3 cut(s)	657, 1188, 1337
BspHI	TCATGA	1 cut(s)	561
BspLI	GGNNCC	7 cut(s)	206, 670, 905, 991, 2015, 2200, 2265
BspMAI	CTGCAG	4 cut(s)	265, 936, 1647, 1951
BspOI	GCTAGC	1 cut(s)	528
BspPI	GGATC	5 cut(s)	143, 199, 212, 1223, 2207
BspQI	GCTCTTC	2 cut(s)	1939, 1948
BspT107I	GGYRCC	1 cut(s)	2198
BspTI	CTTAAG	1 cut(s)	1777
BspTNI	GGTCTC	1 cut(s)	1290
BsrFI	RCCGGY	1 cut(s)	1590
BsrGI	TGTACA	2 cut(s)	487, 1109
BsrI	ACTGG	7 cut(s)	15, 360, 543, 617, 844, 1180, 1869
BssAI	RCCGGY	1 cut(s)	1590
BssECI	CCNNGG	3 cut(s)	306, 1834, 1884
BssMI	GATC	8 cut(s)	135, 204, 685, 724, 818, 1228, 1666, 2212
BssT1I	CCWWGG	2 cut(s)	1834, 1884
Bst2UI	CCWGG	1 cut(s)	308
Bst4CI	ACNGT	4 cut(s)	176, 719, 1167, 1655
Bst6I	CTCTTC	5 cut(s)	1754, 1939, 1948, 2031, 2253
BstAFI	CTTAAG	1 cut(s)	1777
BstAUI	TGTACA	2 cut(s)	487, 1109
BstBAI	YACGTR	1 cut(s)	1213
BstC8I	GCNNGC	3 cut(s)	526, 831, 1570
BstDEI	CTNAG	5 cut(s)	97, 644, 755, 1196, 1324
BstDSI	CCRYGG	1 cut(s)	1834
BstF5I	GGATG	4 cut(s)	316, 1570, 1873, 2053
BstHHI	GCGC	1 cut(s)	2101
BstKTI	GATC	8 cut(s)	138, 207, 688, 727, 821, 1231, 1669, 2215
BstMAI	GTCTC	1 cut(s)	1290
BstMBI	GATC	8 cut(s)	135, 204, 685, 724, 818, 1228, 1666, 2212
BstMWI	GCNNNNNNNGC	5 cut(s)	876, 1415, 1938, 2050, 2205
BstNI	CCWGG	1 cut(s)	308
BstNSI	RCATGY	1 cut(s)	1000
BstSCI	CCNGG	1 cut(s)	306
BstSFI	CTRYAG	7 cut(s)	193, 261, 932, 1378, 1643, 1816, 1947
BstSLI	GKGCMC	1 cut(s)	2201
BstV1I	GCAGC	8 cut(s)	247, 379, 508, 918, 1102, 1331, 1629, 2031
BstV2I	GAAGAC	1 cut(s)	2167
BstX2I	RGATCY	3 cut(s)	135, 204, 1228
BstYI	RGATCY	3 cut(s)	135, 204, 1228
Bsu36I	CCTNAGG	2 cut(s)	97, 1324
BsuRI	GGCC	6 cut(s)	119, 870, 1507, 1793, 1883, 2245
BtgI	CCRYGG	1 cut(s)	1834
BtrI	CACGTC	1 cut(s)	604
BtsCI	GGATG	4 cut(s)	316, 1570, 1873, 2053
BtsI	GCAGTG	1 cut(s)	348
BtsIMutI	CAGTG	4 cut(s)	348, 652, 851, 1193
Cac8I	GCNNGC	3 cut(s)	526, 831, 1570
CciI	TCATGA	1 cut(s)	561
CfoI	GCGC	1 cut(s)	2101
Cfr10I	RCCGGY	1 cut(s)	1590
Cfr13I	GGNCC	5 cut(s)	357, 1505, 1791, 2014, 2243
Csp6I	GTAC	4 cut(s)	488, 1033, 1110, 1862
CspAI	ACCGGT	1 cut(s)	1590
CviAII	CATG	9 cut(s)	354, 562, 792, 813, 997, 1025, 1835, 1929, 1938
CviQI	GTAC	4 cut(s)	488, 1033, 1110, 1862
DdeI	CTNAG	5 cut(s)	97, 644, 755, 1196, 1324
DpnI	GATC	8 cut(s)	137, 206, 687, 726, 820, 1230, 1668, 2214
DpnII	GATC	8 cut(s)	135, 204, 685, 724, 818, 1228, 1666, 2212
DrdI	GACNNNNNNGTC	1 cut(s)	943
DseDI	GACNNNNNNGTC	1 cut(s)	943
EaeI	YGGCCR	2 cut(s)	117, 868
Eam1104I	CTCTTC	5 cut(s)	1754, 1939, 1948, 2031, 2253
EarI	CTCTTC	5 cut(s)	1754, 1939, 1948, 2031, 2253
Eco130I	CCWWGG	2 cut(s)	1834, 1884
Eco24I	GRGCYC	1 cut(s)	673
Eco31I	GGTCTC	1 cut(s)	1290
Eco47I	GGWCC	2 cut(s)	357, 2014
Eco81I	CCTNAGG	2 cut(s)	97, 1324
EcoO109I	RGGNCCY	1 cut(s)	2014
EcoRII	CCWGG	1 cut(s)	306
EcoT14I	CCWWGG	2 cut(s)	1834, 1884
EcoT38I	GRGCYC	1 cut(s)	673
ErhI	CCWWGG	2 cut(s)	1834, 1884
FaeI	CATG	9 cut(s)	357, 565, 795, 816, 1000, 1028, 1838, 1932, 1941
FalI	AAGNNNNNCTT	4 cut(s)	1512, 1544, 2232, 2264
FaqI	GGGAC	1 cut(s)	2027
FatI	CATG	9 cut(s)	353, 561, 791, 812, 996, 1024, 1834, 1928, 1937
FauNDI	CATATG	1 cut(s)	131
FbaI	TGATCA	1 cut(s)	685
FblI	GTMKAC	1 cut(s)	2070
Fnu4HI	GCNGC	9 cut(s)	261, 393, 522, 932, 1116, 1320, 1410, 1643, 2045
FokI	GGATG	4 cut(s)	323, 1577, 1880, 2040
FriOI	GRGCYC	1 cut(s)	673
Fsp4HI	GCNGC	9 cut(s)	261, 393, 522, 932, 1116, 1320, 1410, 1643, 2045
FspBI	CTAG	6 cut(s)	525, 573, 633, 761, 1070, 1679
FspI	TGCGCA	1 cut(s)	2100
GlaI	GCGC	1 cut(s)	2100
GluI	GCNGC	9 cut(s)	261, 393, 522, 932, 1116, 1320, 1410, 1643, 2045
GsaI	CCCAGC	1 cut(s)	301
HaeIII	GGCC	6 cut(s)	119, 870, 1507, 1793, 1883, 2245
HapII	CCGG	3 cut(s)	81, 781, 1591
HhaI	GCGC	1 cut(s)	2101
Hin1II	CATG	9 cut(s)	357, 565, 795, 816, 1000, 1028, 1838, 1932, 1941
Hin6I	GCGC	1 cut(s)	2099
HinP1I	GCGC	1 cut(s)	2099
HindIII	AAGCTT	1 cut(s)	1084
HinfI	GANTC	9 cut(s)	91, 287, 332, 427, 461, 734, 803, 896, 944
HpaII	CCGG	3 cut(s)	81, 781, 1591
HphI	GGTGA	3 cut(s)	161, 598, 1900
Hpy166II	GTNNAC	1 cut(s)	2071
Hpy188I	TCNGA	7 cut(s)	181, 472, 664, 1095, 1186, 1316, 1465
Hpy188III	TCNNGA	9 cut(s)	431, 562, 722, 954, 1070, 1326, 1696, 1709, 2057
Hpy8I	GTNNAC	1 cut(s)	2071
HpyAV	CCTTC	4 cut(s)	334, 639, 1272, 1468
HpyCH4III	ACNGT	4 cut(s)	176, 719, 1167, 1655
HpyCH4IV	ACGT	5 cut(s)	603, 874, 1212, 2113, 2155
HpyF10VI	GCNNNNNNNGC	5 cut(s)	876, 1415, 1938, 2050, 2205
HpyF3I	CTNAG	5 cut(s)	97, 644, 755, 1196, 1324
HpySE526I	ACGT	5 cut(s)	603, 874, 1212, 2113, 2155
Hsp92II	CATG	9 cut(s)	357, 565, 795, 816, 1000, 1028, 1838, 1932, 1941
HspAI	GCGC	1 cut(s)	2099
Ksp22I	TGATCA	1 cut(s)	685
Kzo9I	GATC	8 cut(s)	135, 204, 685, 724, 818, 1228, 1666, 2212
LguI	GCTCTTC	2 cut(s)	1939, 1948
LmnI	GCTCC	4 cut(s)	668, 1316, 1474, 2263
Lsp1109I	GCAGC	8 cut(s)	247, 379, 508, 918, 1102, 1331, 1629, 2031
LweI	GCATC	6 cut(s)	57, 888, 1136, 1555, 1934, 2062
MaeI	CTAG	6 cut(s)	525, 573, 633, 761, 1070, 1679
MaeII	ACGT	5 cut(s)	603, 874, 1212, 2113, 2155
MaeIII	GTNAC	9 cut(s)	66, 281, 346, 545, 599, 604, 708, 1171, 1888
MalI	GATC	8 cut(s)	137, 206, 687, 726, 820, 1230, 1668, 2214
MboI	GATC	8 cut(s)	135, 204, 685, 724, 818, 1228, 1666, 2212
MfeI	CAATTG	1 cut(s)	1355
MflI	RGATCY	3 cut(s)	135, 204, 1228
MhlI	GDGCHC	2 cut(s)	673, 2201
MlsI	TGGCCA	1 cut(s)	870
MluNI	TGGCCA	1 cut(s)	870
MlyI	GAGTC	2 cut(s)	728, 953
MmeI	TCCRAC	1 cut(s)	1017
Mox20I	TGGCCA	1 cut(s)	870
MroXI	GAANNNNTTC	1 cut(s)	628
MscI	TGGCCA	1 cut(s)	870
MslI	CAYNNNNRTG	2 cut(s)	978, 2134
Msp20I	TGGCCA	1 cut(s)	870
MspA1I	CMGCKG	2 cut(s)	301, 1115
MspCI	CTTAAG	1 cut(s)	1777
MspI	CCGG	3 cut(s)	81, 781, 1591
MspR9I	CCNGG	1 cut(s)	308
MunI	CAATTG	1 cut(s)	1355
MvaI	CCWGG	1 cut(s)	308
MwoI	GCNNNNNNNGC	5 cut(s)	876, 1415, 1938, 2050, 2205
NcoI	CCATGG	1 cut(s)	1834
NdeI	CATATG	1 cut(s)	131
NdeII	GATC	8 cut(s)	135, 204, 685, 724, 818, 1228, 1666, 2212
NheI	GCTAGC	1 cut(s)	524
NlaIII	CATG	9 cut(s)	357, 565, 795, 816, 1000, 1028, 1838, 1932, 1941
NlaIV	GGNNCC	7 cut(s)	206, 670, 905, 991, 2015, 2200, 2265
NmuCI	GTSAC	5 cut(s)	346, 599, 604, 708, 1888
NsbI	TGCGCA	1 cut(s)	2100
NspI	RCATGY	1 cut(s)	1000
PacI	TTAATTAA	1 cut(s)	1155
PagI	TCATGA	1 cut(s)	561
PciSI	GCTCTTC	2 cut(s)	1939, 1948
PdmI	GAANNNNTTC	1 cut(s)	628
PfeI	GAWTC	7 cut(s)	91, 287, 332, 427, 461, 803, 896
PinAI	ACCGGT	1 cut(s)	1590
PkrI	GCNGC	9 cut(s)	262, 394, 523, 933, 1117, 1321, 1411, 1644, 2046
PleI	GAGTC	2 cut(s)	728, 952
PpsI	GAGTC	2 cut(s)	728, 952
Ppu21I	YACGTR	1 cut(s)	1213
PpuMI	RGGWCCY	1 cut(s)	2014
PsiI	TTATAA	1 cut(s)	1008
Psp5II	RGGWCCY	1 cut(s)	2014
Psp6I	CCWGG	1 cut(s)	306
PspFI	CCCAGC	1 cut(s)	297
PspGI	CCWGG	1 cut(s)	306
PspN4I	GGNNCC	7 cut(s)	206, 670, 905, 991, 2015, 2200, 2265
PspPI	GGNCC	5 cut(s)	357, 1505, 1791, 2014, 2243
PspPPI	RGGWCCY	1 cut(s)	2014
PstI	CTGCAG	4 cut(s)	265, 936, 1647, 1951
PsuI	RGATCY	3 cut(s)	135, 204, 1228
PvuII	CAGCTG	2 cut(s)	301, 1115
RsaI	GTAC	4 cut(s)	489, 1034, 1111, 1863
RsaNI	GTAC	4 cut(s)	488, 1033, 1110, 1862
RseI	CAYNNNNRTG	2 cut(s)	978, 2134
SapI	GCTCTTC	2 cut(s)	1939, 1948
SatI	GCNGC	9 cut(s)	261, 393, 522, 932, 1116, 1320, 1410, 1643, 2045
Sau3AI	GATC	8 cut(s)	135, 204, 685, 724, 818, 1228, 1666, 2212
Sau96I	GGNCC	5 cut(s)	357, 1505, 1791, 2014, 2243
SchI	GAGTC	2 cut(s)	728, 953
ScrFI	CCNGG	1 cut(s)	308
SduI	GDGCHC	2 cut(s)	673, 2201
SfaNI	GCATC	6 cut(s)	57, 888, 1136, 1555, 1934, 2062
SfcI	CTRYAG	7 cut(s)	193, 261, 932, 1378, 1643, 1816, 1947
SinI	GGWCC	2 cut(s)	357, 2014
SmiMI	CAYNNNNRTG	2 cut(s)	978, 2134
SmlI	CTYRAG	2 cut(s)	1777, 2203
SmoI	CTYRAG	2 cut(s)	1777, 2203
SsiI	CCGC	2 cut(s)	230, 1410
SspMI	CTAG	6 cut(s)	525, 573, 633, 761, 1070, 1679
StyD4I	CCNGG	1 cut(s)	306
StyI	CCWWGG	2 cut(s)	1834, 1884
TaaI	ACNGT	4 cut(s)	176, 719, 1167, 1655
TaiI	ACGT	5 cut(s)	606, 877, 1215, 2116, 2158
TaqI	TCGA	4 cut(s)	517, 589, 1436, 2005
TaqII	GACCGA	1 cut(s)	1272
TatI	WGTACW	2 cut(s)	487, 1109
TauI	GCSGC	1 cut(s)	1412
TfiI	GAWTC	7 cut(s)	91, 287, 332, 427, 461, 803, 896
TscAI	CASTG	4 cut(s)	355, 652, 851, 1200
TseFI	GTSAC	5 cut(s)	346, 599, 604, 708, 1888
TseI	GCWGC	8 cut(s)	260, 392, 521, 931, 1115, 1319, 1642, 2044
Tsp45I	GTSAC	5 cut(s)	346, 599, 604, 708, 1888
TspGWI	ACGGA	3 cut(s)	197, 423, 671
TspRI	CASTG	4 cut(s)	355, 652, 851, 1200
Vha464I	CTTAAG	1 cut(s)	1777
VpaK11BI	GGWCC	2 cut(s)	357, 2014
XapI	RAATTY	5 cut(s)	1390, 1823, 1840, 1873, 1895
XbaI	TCTAGA	1 cut(s)	1069
XceI	RCATGY	1 cut(s)	1000
XmiI	GTMKAC	1 cut(s)	2070
XmnI	GAANNNNTTC	1 cut(s)	628
XspI	CTAG	6 cut(s)	525, 573, 633, 761, 1070, 1679

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.