Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0005116

Physical Location & Seq

Genomic Coordinates Forward (+)

8946520 .. 8959126

Gene Structure 12607 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1074400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2904 bp

ATGTCTAGCTCTACAAAAGCTTTGGATCCGGCTTTTCAGGGATCAGGTCAAAGGATAGGGGCTGAAATTTGGCGAATTGAGGACTTCCAACCAGTTCCTTTGCCAAAATCTGAACATGGAAAATTCTATATGGGGGATTCTTATATTGTCTTGCAGACAACACAAAACAAAGGGGGCGCTTATCTCTATGACATACACTTCTGGATTGGGAAAGATACAAGTCAGGATGAAGCTGGGACAGCAGCTATTAAGACTGTTGAGCTAGATGCAGTTCTTGGAGGACGTGCAGTGCAACATCGAGAAATTCAAGGCCATGAATCTGACAAATTTTTGTCATACTTCAAACCTTGTATTATACCATTGGAGGGGGGTGTTGCTTCTGGATTTAAAAAGGTTGAGGAAGACGAATTTGAAACTCGGTTGTATATCTGCAAAGGAAAACGAGTTGTCAGAATGAAGCAGGTTCCTTTTGCGCGTTCCTCTCTGAATCATGATGATGTGTTTATCCTGGACACTGAAAACAAGATCTTTCAGTTCAACGGAGCAAATTCTAATATCCAGGAGAGGGCGAAGGCTTTGGAAGTAATCCAGTTTTTGAAGGAAAAGTATCATCATGGAACCTGTGATGTTGCAATAGTTGATGATGGGAAGCTAGATACAGAGTCCGATTCGGGTGAATTTTGGGTCCTAATGGGTGGATTTGCTCCAATTGGAAAGAAGGTAATCACTGAAGATGATGTGGTTCCAGAAGCTACTCCTGCTATACTGTACAGCATAACCGAGGGTGAAGTGAAGACTGTAGAAGGTGAACTATCCAAATCCTTGCTTGAAAACAACAAATGCTACTTACTGGACTGCGGTTCAGAGGTGTTTGTTTGGGTTGGCCGGGTGACACAAGTGGAGGACAGAAAATCTGCTAGCCAAGCAGCTGAGGAGTTTCTTGCTACTCAGAATAGACCAAAGTCAACACGAATAACCCGGGTTATTCAAGGTTATGAAACACATTCATTCAAGTCCAACTTCGATTCTTGGCCGTCAGGATCAGCAACTTCTGGTACTGAAGAGGGAAGAGGAAAAGTAGCAGCTTTGCTGAAGCAACAAGGTGTTGGTTTAAAGGGTGTTGCAAAAGGTGCTCCCGTAAATGAGGAGGTCCCACCTTTGCTAGAAGGAGGTGGAAAGATGGAGGTATGGTGTATCAATGGTGGTGCCAAGACTCCTTTGCCTAAGGAGGATATTGGTAAATTTTACAGTGGAGATTGCTATATCATTCTCTATACTTACCACTCTGGTGATAGAAAAGAAGATTACTTTTTGTGCTGTTGGTTTGGAAAAAATAGTATTGAGGAGGACCAGAAAACTGCTTCTCATTTGGCTAGCACAATGTCCAACTCGCTGAAGGGAAGACCAGTGCAGGGTCACATATTTCAAGGTAAAGAGCCACCACAGTTAGTAGCACTTTTTCAGCCTATGGTGGTCCTGAAGGGTGGCTTGAGCTCTGGTTATAAGAAATCGGTTGAGGAGAAAGGTCTGACAGATGAAACTTATACCGCAGATTGTGTGGCACTCTTTCGGTTATCTGGAACTTATGTTCATAACAGTAAAGCAGTGCAAGTTGATGCGGTGGCAACATCATTGAACTCTACCGAGTGTTTCATTCTGCAATCTGGGTCCTCAATGTTTGCCTGGAATGGAAATCAATGCACCATTGAACAGCAACAGTTAGCAGCAAAACTTGCAGAATTTTTGAAGCCTGGAGTTACTCTAAAACATGCTAAAGAGGGAACTGAGAGCTCATCCTTCTGGTTTGCTCTAGGAGGGAAACAAAGTTACACCAGCAATAAAGTTTCTCAGGAGATTGTCAGAGATCCCCACCTGTTCTCATTCTCGTTCAACAGAGGAAAGTTTCAGGTGGAGGAAATTTACAACTTCACTCAGGATGATCTGTTGACAGAGGATATCCTTATACTTGACACACATGCCGAAGTGTTTGTTTGGGTTGGTCAATGTGTAGACTCGAAAGAAAAGCAAAATGCTTTTGAAATTGGCAAGAAATACATAGCGCTGGCGGCATCTCTGGAGGGGTTGGCTCCTAATGTACCATTATACAAAGTTACTGAAGGAAATGAACCTCGCTTCTTTACAACATACTTTTCATGGGATCTTTCAAAAGCCACTGTTCAAGGAAACTCATTCCTCAAGAAGGTGTCAATACTCTTTGGAATTGGCCATGCTGTGGAGGACAAGTCCACTGGGAACCAAGGAGGACCAAGGCAAAGAGCTGAAGCCTTAGCTGCTTTATCATCTGCATTCAATCCATCCTCCGGAAAGTCATCCCAAGCGGGCCAGGATAAGTCCGATGGATCGAGTGAAGGAGGACCAAGGCAAAGAGCAGAAGCTTTAGCTGCTTTATCTTCTGCATTTAATTCATCCCCTGGAAACAAACCTTCTCTTCCCAAGCCGTCTGCATCAGGTCAAGGAACACAAAGAGCTGCAGCAGTTGCTGCGCTTTCAAATGTTCTTACAGCTGAAAAGAGTAAGTTAACACCAGATGCTTCTCCTGTGCAAAGCCCTCCCTCAGAAACTAGTGCTTCTGAGGGTCCTCAGGAACTTCCTGAAGTCAAGGAAACAGGTGAATCTGCACCTGCTTCCGAAAGCAGTGAGGATGATTCAAAACAAAAGACACTGCAGGACGAGAGTGAGAGTGAAAGTAGTCGCAGTACATTCAGTTATGACCAACTGAGGGCTAAATCTGACAATCCTGTAACCGGCATTGATTTTAAGAGAAGAGAGACCTATCTCTCTGACGAGGAGTTTCAGACTATATTCGGGATGCCAAAAGATGCTTTCTATCAACTGCCAAAGTGGAAGCAAGACATGCAGAAGAGGAAAGCTGATTTATTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001558 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005884 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008092 GO:0008150 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009987 GO:0010639 GO:0010817 GO:0015629 GO:0016043 GO:0022607 GO:0030029 GO:0030036 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0031333 GO:0032271 GO:0032272 GO:0032432 GO:0032535 GO:0032879 GO:0032956 GO:0032970 GO:0033043 GO:0040008 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043242 GO:0043244 GO:0043254 GO:0044085 GO:0044087 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0044877 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051014 GO:0051015 GO:0051017 GO:0051049 GO:0051128 GO:0051129 GO:0051493 GO:0051494 GO:0051693 GO:0061572 GO:0065007 GO:0065008 GO:0071840 GO:0090066 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0110053 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:2000012

KEGG Pathways

Metabolic & Signaling

ko04666 ko04810 ko05203 map04666 map04810 map05203

Pfam Domains

Protein Families

Gelsolin VHP

Protein Analysis

968

Amino Acids

105.95

Weight (kDa)

5.43

Isoelectric Point (pI)

43.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gelsolin	PF00626	30 - 111	1.5e-12	Gelsolin repeat
Gelsolin	PF00626	149 - 217	6.4e-12	Gelsolin repeat
Gelsolin	PF00626	266 - 337	1.2e-12	Gelsolin repeat
Gelsolin	PF00626	408 - 482	7.3e-08	Gelsolin repeat
Gelsolin	PF00626	534 - 600	1.2e-06	Gelsolin repeat
Gelsolin	PF00626	635 - 711	9.4e-06	Gelsolin repeat
VHP	PF02209	932 - 967	1.3e-16	Villin headpiece domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005116)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41740 AT2G41740 AT3G57410 AT3G57410 AT3G57410 AT3G57410 AT3G57410 AT3G57410 AT3G57410 AT3G57410 AT3G57410 AT3G57410
fragaria_vesca	FvH4_6g23900 FvH4_6g23900 FvH4_6g23900 FvH4_6g23900
malus_domestica	MD12G1074400.v1.1 MD14G1072600.v1.1
prunus_persica	Prupe.7G059400_v2.0.a1
pyrus_communis	pycom12g06410 pycom14g06250
rosa_chinensis	RchiOBHm_Chr3g0482581
rosa_laevigata	RLG00000023341
rosa_roxburghii	Rroxscaffold_6G00399140
rosa_rugosa	Rorug03G0197000 Rorug03G0197100
rosa_samantha	Rh3AG248500 Rh3BG283600 Rh3DG277400
rosa_wichuraiana	Rw3G022440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1503
AarI	CACCTGC	1 cut(s)	2650
Acc36I	ACCTGC	2 cut(s)	451, 2650
AccB1I	GGYRCC	1 cut(s)	1205
AccB7I	CCANNNNNTGG	1 cut(s)	2233
AccI	GTMKAC	1 cut(s)	2010
AccII	CGCG	1 cut(s)	475
AccIII	TCCGGA	1 cut(s)	2321
AciI	CCGC	5 cut(s)	858, 1548, 1619, 2066, 2339
AclWI	GGATC	7 cut(s)	20, 33, 49, 1048, 1859, 2166, 2368
AcoI	YGGCCR	3 cut(s)	883, 1031, 2224
AcuI	CTGAAG	8 cut(s)	750, 1080, 1112, 1415, 1499, 2136, 2301, 2634
AfaI	GTAC	4 cut(s)	770, 1057, 2097, 2719
AfeI	AGCGCT	1 cut(s)	2061
AfiI	CCNNNNNNNGG	8 cut(s)	365, 565, 1412, 2200, 2233, 2321, 2740, 2765
AhlI	ACTAGT	1 cut(s)	2582
AjiI	CACGTC	1 cut(s)	284
AjnI	CCWGG	6 cut(s)	507, 558, 1682, 1750, 2343, 2431
AleI	CACNNNNGTG	1 cut(s)	1287
AloI	GAACNNNNNNTCC	2 cut(s)	1572, 1604
Alw21I	GWGCWC	3 cut(s)	1135, 1496, 1793
Alw26I	GTCTC	1 cut(s)	2783
AlwI	GGATC	7 cut(s)	20, 33, 49, 1048, 1859, 2166, 2368
AlwNI	CAGNNNCTG	1 cut(s)	2501
Ama87I	CYCGRG	1 cut(s)	978
Aor13HI	TCCGGA	1 cut(s)	2321
Aor51HI	AGCGCT	1 cut(s)	2061
AoxI	GGCC	5 cut(s)	310, 883, 1031, 2224, 2341
ApeKI	GCWGC	9 cut(s)	242, 926, 1082, 1724, 2291, 2402, 2489, 2492, 2501
AspLEI	GCGC	4 cut(s)	179, 475, 2062, 2506
AspS9I	GGNCC	9 cut(s)	685, 1150, 1348, 1474, 1668, 2264, 2341, 2375, 2597
AsuC2I	CCSGG	3 cut(s)	887, 979, 980
AsuHPI	GGTGA	6 cut(s)	686, 797, 818, 901, 1301, 2642
AsuNHI	GCTAGC	2 cut(s)	917, 1373
AvaI	CYCGRG	1 cut(s)	978
AvaII	GGWCC	8 cut(s)	685, 1150, 1348, 1474, 1668, 2264, 2375, 2597
AxyI	CCTNAGG	2 cut(s)	1224, 2601
BalI	TGGCCA	1 cut(s)	2226
BamHI	GGATCC	1 cut(s)	25
BanI	GGYRCC	1 cut(s)	1205
BanII	GRGCYC	2 cut(s)	1496, 1793
BbsI	GAAGAC	3 cut(s)	408, 800, 1408
Bbv12I	GWGCWC	3 cut(s)	1135, 1496, 1793
BbvCI	CCTCAGC	1 cut(s)	930
BbvI	GCAGC	9 cut(s)	254, 938, 1094, 1736, 2278, 2389, 2476, 2488, 2504
BccI	CCATC	4 cut(s)	638, 1174, 2323, 2351
BceAI	ACGGC	2 cut(s)	1018, 2443
BciT130I	CCWGG	6 cut(s)	509, 560, 1684, 1752, 2345, 2433
BcnI	CCSGG	3 cut(s)	887, 979, 980
BcoDI	GTCTC	1 cut(s)	2783
BcuI	ACTAGT	1 cut(s)	2582
BfaI	CTAG	9 cut(s)	6, 263, 653, 918, 1163, 1374, 1811, 2583, 2902
BfmI	CTRYAG	3 cut(s)	798, 2490, 2684
BfoI	RGCGCY	2 cut(s)	180, 2063
BfuAI	ACCTGC	2 cut(s)	451, 2650
BglII	AGATCT	1 cut(s)	525
Bme1390I	CCNGG	9 cut(s)	509, 560, 887, 979, 980, 1684, 1752, 2345, 2433
Bme18I	GGWCC	8 cut(s)	685, 1150, 1348, 1474, 1668, 2264, 2375, 2597
BmeT110I	CYCGRG	1 cut(s)	978
BmgBI	CACGTC	1 cut(s)	284
BmgT120I	GGNCC	9 cut(s)	685, 1150, 1348, 1474, 1668, 2264, 2341, 2375, 2597
BmrFI	CCNGG	9 cut(s)	509, 560, 887, 979, 980, 1684, 1752, 2345, 2433
BmrI	ACTGGG	1 cut(s)	2259
BmsI	GCATC	7 cut(s)	256, 1606, 2078, 2474, 2539, 2820, 2830
BmtI	GCTAGC	2 cut(s)	921, 1377
BmuI	ACTGGG	1 cut(s)	2259
BoxI	GACNNNNGTC	1 cut(s)	961
BpiI	GAAGAC	3 cut(s)	408, 800, 1408
BpmI	CTGGAG	2 cut(s)	1773, 2096
Bpu10I	CCTNAGC	2 cut(s)	930, 2287
BpuEI	CTTGAG	2 cut(s)	1510, 2180
BpuMI	CCSGG	3 cut(s)	887, 979, 980
BsaI	GGTCTC	1 cut(s)	2783
BsaJI	CCNNGG	6 cut(s)	780, 978, 2257, 2267, 2378, 2431
BsaWI	WCCGGW	1 cut(s)	2321
BsaXI	ACNNNNNCTCC	4 cut(s)	1175, 1205, 2228, 2258
Bsc4I	CCNNNNNNNGG	8 cut(s)	365, 565, 1412, 2200, 2233, 2321, 2740, 2765
Bse118I	RCCGGY	1 cut(s)	2765
Bse1I	ACTGG	5 cut(s)	92, 589, 855, 1406, 2254
Bse21I	CCTNAGG	2 cut(s)	1224, 2601
BseAI	TCCGGA	1 cut(s)	2321
BseBI	CCWGG	6 cut(s)	509, 560, 1684, 1752, 2345, 2433
BseDI	CCNNGG	6 cut(s)	780, 978, 2257, 2267, 2378, 2431
BseGI	GGATG	8 cut(s)	232, 1793, 1942, 2315, 2330, 2426, 2668, 2835
BseLI	CCNNNNNNNGG	8 cut(s)	365, 565, 1412, 2200, 2233, 2321, 2740, 2765
BseMII	CTCAG	9 cut(s)	921, 962, 1776, 1862, 1946, 2583, 2589, 2615, 2729
BseNI	ACTGG	5 cut(s)	92, 589, 855, 1406, 2254
BseRI	GAGGAG	5 cut(s)	947, 1160, 1358, 1532, 2822
BseXI	GCAGC	9 cut(s)	254, 938, 1094, 1736, 2278, 2389, 2476, 2488, 2504
BseYI	CCCAGC	1 cut(s)	233
BsgI	GTGCAG	3 cut(s)	306, 1430, 2622
Bsh1236I	CGCG	1 cut(s)	475
BshFI	GGCC	5 cut(s)	312, 885, 1033, 2226, 2343
BshNI	GGYRCC	1 cut(s)	1205
BsiHKAI	GWGCWC	3 cut(s)	1135, 1496, 1793
BsiHKCI	CYCGRG	1 cut(s)	978
BsiSI	CCGG	5 cut(s)	29, 886, 979, 2322, 2766
BslFI	GGGAC	2 cut(s)	250, 1136
BslI	CCNNNNNNNGG	8 cut(s)	365, 565, 1412, 2200, 2233, 2321, 2740, 2765
BsmAI	GTCTC	1 cut(s)	2783
BsmFI	GGGAC	2 cut(s)	250, 1136
BsmI	GAATGC	1 cut(s)	2306
BsnI	GGCC	5 cut(s)	312, 885, 1033, 2226, 2343
Bso31I	GGTCTC	1 cut(s)	2783
BsoBI	CYCGRG	1 cut(s)	978
Bsp1286I	GDGCHC	3 cut(s)	1135, 1496, 1793
Bsp13I	TCCGGA	1 cut(s)	2321
Bsp1407I	TGTACA	1 cut(s)	768
Bsp143I	GATC	8 cut(s)	25, 41, 525, 1040, 1864, 1939, 2158, 2360
BspACI	CCGC	5 cut(s)	858, 1548, 1619, 2066, 2339
BspANI	GGCC	5 cut(s)	312, 885, 1033, 2226, 2343
BspCNI	CTCAG	9 cut(s)	922, 961, 1777, 1861, 1945, 2584, 2588, 2614, 2730
BspEI	TCCGGA	1 cut(s)	2321
BspFNI	CGCG	1 cut(s)	475
BspHI	TCATGA	1 cut(s)	490
BspMAI	CTGCAG	2 cut(s)	2494, 2688
BspMI	ACCTGC	2 cut(s)	451, 2650
BspOI	GCTAGC	2 cut(s)	921, 1377
BspPI	GGATC	7 cut(s)	20, 33, 49, 1048, 1859, 2166, 2368
BspT107I	GGYRCC	1 cut(s)	1205
BspTNI	GGTCTC	1 cut(s)	2783
BsrFI	RCCGGY	1 cut(s)	2765
BsrGI	TGTACA	1 cut(s)	768
BsrI	ACTGG	5 cut(s)	92, 589, 855, 1406, 2254
BssAI	RCCGGY	1 cut(s)	2765
BssECI	CCNNGG	6 cut(s)	780, 978, 2257, 2267, 2378, 2431
BssMI	GATC	8 cut(s)	25, 41, 525, 1040, 1864, 1939, 2158, 2360
BssT1I	CCWWGG	3 cut(s)	2257, 2267, 2378
Bst2UI	CCWGG	6 cut(s)	509, 560, 1684, 1752, 2345, 2433
Bst4CI	ACNGT	8 cut(s)	256, 768, 799, 1250, 1446, 1598, 1719, 2176
Bst6I	CTCTTC	5 cut(s)	1056, 1063, 2454, 2779, 2876
BstAPI	GCANNNNNTGC	5 cut(s)	1130, 1733, 2498, 2501, 2875
BstAUI	TGTACA	1 cut(s)	768
BstC8I	GCNNGC	4 cut(s)	919, 1375, 2064, 2341
BstENI	CCTNNNNNAGG	1 cut(s)	2198
BstF5I	GGATG	8 cut(s)	232, 1793, 1942, 2315, 2330, 2426, 2668, 2835
BstFNI	CGCG	1 cut(s)	475
BstH2I	RGCGCY	2 cut(s)	180, 2063
BstHHI	GCGC	4 cut(s)	179, 475, 2062, 2506
BstKTI	GATC	8 cut(s)	28, 44, 528, 1043, 1867, 1942, 2161, 2363
BstMAI	GTCTC	1 cut(s)	2783
BstMBI	GATC	8 cut(s)	25, 41, 525, 1040, 1864, 1939, 2158, 2360
BstNI	CCWGG	6 cut(s)	509, 560, 1684, 1752, 2345, 2433
BstNSI	RCATGY	3 cut(s)	1772, 1979, 2878
BstPAI	GACNNNNGTC	1 cut(s)	961
BstSCI	CCNGG	9 cut(s)	507, 558, 885, 977, 978, 1682, 1750, 2343, 2431
BstSFI	CTRYAG	3 cut(s)	798, 2490, 2684
BstUI	CGCG	1 cut(s)	475
BstV1I	GCAGC	9 cut(s)	254, 938, 1094, 1736, 2278, 2389, 2476, 2488, 2504
BstV2I	GAAGAC	3 cut(s)	408, 800, 1408
BstX2I	RGATCY	4 cut(s)	25, 525, 1864, 2158
BstYI	RGATCY	4 cut(s)	25, 525, 1864, 2158
Bsu36I	CCTNAGG	2 cut(s)	1224, 2601
BsuRI	GGCC	5 cut(s)	312, 885, 1033, 2226, 2343
BtrI	CACGTC	1 cut(s)	284
BtsCI	GGATG	8 cut(s)	232, 1793, 1942, 2315, 2330, 2426, 2668, 2835
BtsI	GCAGTG	4 cut(s)	294, 1611, 2662, 2681
BveI	ACCTGC	2 cut(s)	451, 2650
Cac8I	GCNNGC	4 cut(s)	919, 1375, 2064, 2341
CaiI	CAGNNNCTG	1 cut(s)	2501
CciI	TCATGA	1 cut(s)	490
CfoI	GCGC	4 cut(s)	179, 475, 2062, 2506
Cfr10I	RCCGGY	1 cut(s)	2765
Cfr13I	GGNCC	9 cut(s)	685, 1150, 1348, 1474, 1668, 2264, 2341, 2375, 2597
Cfr9I	CCCGGG	1 cut(s)	978
Csp6I	GTAC	4 cut(s)	769, 1056, 2096, 2718
CviAII	CATG	9 cut(s)	116, 314, 491, 614, 1769, 1976, 2154, 2228, 2875
CviQI	GTAC	4 cut(s)	769, 1056, 2096, 2718
DpnI	GATC	8 cut(s)	27, 43, 527, 1042, 1866, 1941, 2160, 2362
DpnII	GATC	8 cut(s)	25, 41, 525, 1040, 1864, 1939, 2158, 2360
DraI	TTTAAA	2 cut(s)	388, 1113
EaeI	YGGCCR	3 cut(s)	883, 1031, 2224
Eam1104I	CTCTTC	5 cut(s)	1056, 1063, 2454, 2779, 2876
EarI	CTCTTC	5 cut(s)	1056, 1063, 2454, 2779, 2876
Ecl136II	GAGCTC	2 cut(s)	1494, 1791
Eco130I	CCWWGG	3 cut(s)	2257, 2267, 2378
Eco24I	GRGCYC	2 cut(s)	1496, 1793
Eco31I	GGTCTC	1 cut(s)	2783
Eco32I	GATATC	1 cut(s)	1957
Eco47I	GGWCC	8 cut(s)	685, 1150, 1348, 1474, 1668, 2264, 2375, 2597
Eco47III	AGCGCT	1 cut(s)	2061
Eco53kI	GAGCTC	2 cut(s)	1494, 1791
Eco57I	CTGAAG	8 cut(s)	750, 1080, 1112, 1415, 1499, 2136, 2301, 2634
Eco81I	CCTNAGG	2 cut(s)	1224, 2601
Eco88I	CYCGRG	1 cut(s)	978
EcoICRI	GAGCTC	2 cut(s)	1494, 1791
EcoNI	CCTNNNNNAGG	1 cut(s)	2198
EcoO109I	RGGNCCY	4 cut(s)	685, 1150, 1668, 2597
EcoRII	CCWGG	6 cut(s)	507, 558, 1682, 1750, 2343, 2431
EcoRV	GATATC	1 cut(s)	1957
EcoT14I	CCWWGG	3 cut(s)	2257, 2267, 2378
EcoT38I	GRGCYC	2 cut(s)	1496, 1793
ErhI	CCWWGG	3 cut(s)	2257, 2267, 2378
FaeI	CATG	9 cut(s)	119, 317, 494, 617, 1772, 1979, 2157, 2231, 2878
FalI	AAGNNNNNCTT	4 cut(s)	1004, 1036, 1472, 1504
FaqI	GGGAC	2 cut(s)	250, 1136
FatI	CATG	9 cut(s)	115, 313, 490, 613, 1768, 1975, 2153, 2227, 2874
FauI	CCCGC	1 cut(s)	2332
FblI	GTMKAC	1 cut(s)	2010
FokI	GGATG	8 cut(s)	239, 1780, 1949, 2302, 2317, 2413, 2675, 2842
FriOI	GRGCYC	2 cut(s)	1496, 1793
FspBI	CTAG	9 cut(s)	6, 263, 653, 918, 1163, 1374, 1811, 2583, 2902
GlaI	GCGC	4 cut(s)	178, 474, 2061, 2505
GsaI	CCCAGC	1 cut(s)	237
GsuI	CTGGAG	2 cut(s)	1773, 2096
HaeII	RGCGCY	2 cut(s)	180, 2063
HaeIII	GGCC	5 cut(s)	312, 885, 1033, 2226, 2343
HapII	CCGG	5 cut(s)	29, 886, 979, 2322, 2766
HhaI	GCGC	4 cut(s)	179, 475, 2062, 2506
Hin1II	CATG	9 cut(s)	119, 317, 494, 617, 1772, 1979, 2157, 2231, 2878
Hin6I	GCGC	4 cut(s)	177, 473, 2060, 2504
HinP1I	GCGC	4 cut(s)	177, 473, 2060, 2504
HincII	GTYRAC	3 cut(s)	966, 1947, 2541
HindII	GTYRAC	3 cut(s)	966, 1947, 2541
HindIII	AAGCTT	2 cut(s)	18, 2394
HpaI	GTTAAC	1 cut(s)	2541
HpaII	CCGG	5 cut(s)	29, 886, 979, 2322, 2766
HphI	GGTGA	6 cut(s)	686, 797, 818, 901, 1301, 2642
Hpy166II	GTNNAC	6 cut(s)	809, 966, 1947, 2011, 2247, 2541
Hpy8I	GTNNAC	6 cut(s)	809, 966, 1947, 2011, 2247, 2541
HpyCH4III	ACNGT	8 cut(s)	256, 768, 799, 1250, 1446, 1598, 1719, 2176
HpyCH4IV	ACGT	1 cut(s)	283
HpySE526I	ACGT	1 cut(s)	283
Hsp92II	CATG	9 cut(s)	119, 317, 494, 617, 1772, 1979, 2157, 2231, 2878
HspAI	GCGC	4 cut(s)	177, 473, 2060, 2504
Kpn2I	TCCGGA	1 cut(s)	2321
KspAI	GTTAAC	1 cut(s)	2541
Kzo9I	GATC	8 cut(s)	25, 41, 525, 1040, 1864, 1939, 2158, 2360
LmnI	GCTCC	4 cut(s)	542, 709, 1138, 2092
Lsp1109I	GCAGC	9 cut(s)	254, 938, 1094, 1736, 2278, 2389, 2476, 2488, 2504
LweI	GCATC	7 cut(s)	256, 1606, 2078, 2474, 2539, 2820, 2830
MaeI	CTAG	9 cut(s)	6, 263, 653, 918, 1163, 1374, 1811, 2583, 2902
MaeII	ACGT	1 cut(s)	283
MaeIII	GTNAC	6 cut(s)	889, 1415, 1756, 1826, 2110, 2761
MalI	GATC	8 cut(s)	27, 43, 527, 1042, 1866, 1941, 2160, 2362
MboI	GATC	8 cut(s)	25, 41, 525, 1040, 1864, 1939, 2158, 2360
MfeI	CAATTG	1 cut(s)	708
MflI	RGATCY	4 cut(s)	25, 525, 1864, 2158
MhlI	GDGCHC	3 cut(s)	1135, 1496, 1793
MlsI	TGGCCA	1 cut(s)	2226
MluNI	TGGCCA	1 cut(s)	2226
MlyI	GAGTC	3 cut(s)	671, 1207, 2006
MmeI	TCCRAC	3 cut(s)	112, 1041, 1410
Mox20I	TGGCCA	1 cut(s)	2226
MroI	TCCGGA	1 cut(s)	2321
MscI	TGGCCA	1 cut(s)	2226
MseI	TTAA	6 cut(s)	249, 387, 1112, 2421, 2540, 2778
MslI	CAYNNNNRTG	2 cut(s)	495, 1287
Msp20I	TGGCCA	1 cut(s)	2226
MspA1I	CMGCKG	2 cut(s)	929, 2525
MspI	CCGG	5 cut(s)	29, 886, 979, 2322, 2766
MspR9I	CCNGG	9 cut(s)	509, 560, 887, 979, 980, 1684, 1752, 2345, 2433
MunI	CAATTG	1 cut(s)	708
Mva1269I	GAATGC	1 cut(s)	2306
MvaI	CCWGG	6 cut(s)	509, 560, 1684, 1752, 2345, 2433
MvnI	CGCG	1 cut(s)	475
NciI	CCSGG	3 cut(s)	887, 979, 980
NdeII	GATC	8 cut(s)	25, 41, 525, 1040, 1864, 1939, 2158, 2360
NheI	GCTAGC	2 cut(s)	917, 1373
NlaIII	CATG	9 cut(s)	119, 317, 494, 617, 1772, 1979, 2157, 2231, 2878
NmuCI	GTSAC	2 cut(s)	889, 1415
NspI	RCATGY	3 cut(s)	1772, 1979, 2878
OliI	CACNNNNGTG	1 cut(s)	1287
PagI	TCATGA	1 cut(s)	490
PaqCI	CACCTGC	1 cut(s)	2650
PctI	GAATGC	1 cut(s)	2306
PfeI	GAWTC	7 cut(s)	137, 317, 487, 668, 1025, 2633, 2666
PflMI	CCANNNNNTGG	1 cut(s)	2233
PfoI	TCCNGGA	2 cut(s)	507, 558
PleI	GAGTC	3 cut(s)	670, 1207, 2006
PpsI	GAGTC	3 cut(s)	670, 1207, 2006
PpuMI	RGGWCCY	4 cut(s)	685, 1150, 1668, 2597
PshAI	GACNNNNGTC	1 cut(s)	961
PsiI	TTATAA	1 cut(s)	1503
Psp124BI	GAGCTC	2 cut(s)	1496, 1793
Psp5II	RGGWCCY	4 cut(s)	685, 1150, 1668, 2597
Psp6I	CCWGG	6 cut(s)	507, 558, 1682, 1750, 2343, 2431
PspFI	CCCAGC	1 cut(s)	233
PspGI	CCWGG	6 cut(s)	507, 558, 1682, 1750, 2343, 2431
PspPI	GGNCC	9 cut(s)	685, 1150, 1348, 1474, 1668, 2264, 2341, 2375, 2597
PspPPI	RGGWCCY	4 cut(s)	685, 1150, 1668, 2597
PstI	CTGCAG	2 cut(s)	2494, 2688
PstNI	CAGNNNCTG	1 cut(s)	2501
PsuI	RGATCY	4 cut(s)	25, 525, 1864, 2158
PvuII	CAGCTG	2 cut(s)	929, 2525
RsaI	GTAC	4 cut(s)	770, 1057, 2097, 2719
RsaNI	GTAC	4 cut(s)	769, 1056, 2096, 2718
RseI	CAYNNNNRTG	2 cut(s)	495, 1287
SacI	GAGCTC	2 cut(s)	1496, 1793
SaqAI	TTAA	6 cut(s)	249, 387, 1112, 2421, 2540, 2778
Sau3AI	GATC	8 cut(s)	25, 41, 525, 1040, 1864, 1939, 2158, 2360
Sau96I	GGNCC	9 cut(s)	685, 1150, 1348, 1474, 1668, 2264, 2341, 2375, 2597
SchI	GAGTC	3 cut(s)	671, 1207, 2006
ScrFI	CCNGG	9 cut(s)	509, 560, 887, 979, 980, 1684, 1752, 2345, 2433
SduI	GDGCHC	3 cut(s)	1135, 1496, 1793
SfaNI	GCATC	7 cut(s)	256, 1606, 2078, 2474, 2539, 2820, 2830
SfcI	CTRYAG	3 cut(s)	798, 2490, 2684
SinI	GGWCC	8 cut(s)	685, 1150, 1348, 1474, 1668, 2264, 2375, 2597
SmaI	CCCGGG	1 cut(s)	980
SmiMI	CAYNNNNRTG	2 cut(s)	495, 1287
SmlI	CTYRAG	2 cut(s)	1489, 2195
SmoI	CTYRAG	2 cut(s)	1489, 2195
SpeI	ACTAGT	1 cut(s)	2582
SsiI	CCGC	5 cut(s)	858, 1548, 1619, 2066, 2339
SspMI	CTAG	9 cut(s)	6, 263, 653, 918, 1163, 1374, 1811, 2583, 2902
SstI	GAGCTC	2 cut(s)	1496, 1793
StyD4I	CCNGG	9 cut(s)	507, 558, 885, 977, 978, 1682, 1750, 2343, 2431
StyI	CCWWGG	3 cut(s)	2257, 2267, 2378
TaaI	ACNGT	8 cut(s)	256, 768, 799, 1250, 1446, 1598, 1719, 2176
TaiI	ACGT	1 cut(s)	286
TaqI	TCGA	4 cut(s)	298, 1023, 2015, 2363
TatI	WGTACW	2 cut(s)	768, 2717
TauI	GCSGC	1 cut(s)	2069
TfiI	GAWTC	7 cut(s)	137, 317, 487, 668, 1025, 2633, 2666
Tru1I	TTAA	6 cut(s)	249, 387, 1112, 2421, 2540, 2778
Tru9I	TTAA	6 cut(s)	249, 387, 1112, 2421, 2540, 2778
TseFI	GTSAC	2 cut(s)	889, 1415
TseI	GCWGC	9 cut(s)	242, 926, 1082, 1724, 2291, 2402, 2489, 2492, 2501
Tsp45I	GTSAC	2 cut(s)	889, 1415
TspGWI	ACGGA	1 cut(s)	555
TspMI	CCCGGG	1 cut(s)	978
Van91I	CCANNNNNTGG	1 cut(s)	2233
VpaK11BI	GGWCC	8 cut(s)	685, 1150, 1348, 1474, 1668, 2264, 2375, 2597
XagI	CCTNNNNNAGG	1 cut(s)	2198
XceI	RCATGY	3 cut(s)	1772, 1979, 2878
XmaI	CCCGGG	1 cut(s)	978
XmiI	GTMKAC	1 cut(s)	2010
XspI	CTAG	9 cut(s)	6, 263, 653, 918, 1163, 1374, 1811, 2583, 2902

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.