MD12G1087500.v1.1

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0006942
Physical Location & Seq
Forward (+)
10766937 .. 10770124
3188 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1087500.v1.1.491

Sequence Viewer

Length: 2880 bp
ATGGATGATAAGGAGCTTGAAGTTGCAGCTGATTTTGGTTCCAACCAAATGCCTGAGAAATTGTCAACGACCACCATTGACGCACCTTCTGTTTTGCAAAACCAAAACGGCAGAAGTCAAGGAGAGGGAGCACAAGAGCTTCTGCAGCTGCAAAAGAAGAAGAGGAAGAAACTGAAAAAGTTGACATCGATCAAGCTCTCCAAATCACCAAGTTTGAAGCCGTCTCGAAGAAGATCCAATCACCATGTATCATCCACTGATGATTCTTGTGATACAAGTACAGAAAACTTACAGAATTCAGCAAGAACATTAGCGAGGCGGTCTAGTTTTAAGCCTGCAAAGAATCTGACGAGAATTTCCAGTTTAAAATTTAGAAACCCTTTGATGAGGAAGTGCTCGGGAGGAACCCATATGAATAATATTGATCTCAATAAATCAAGAATGTCTAAGCCTGCAGATTCTGCCATCTCATCCACTGATGCAGATTCATCATCACCTACTTGTTCCAGTAGCAGTACTAGTATTGAGACTAGGAAAAGAGCAGTTTGGTCAAAGCTGAACTCGAAAACTCCTCCTGGTCAGTGTTCACCTTTGAATTTCTCACCAAAATCCAACAAGTGTTCCAGCAGGAACCACTCTGAAATCGTTCAAGTTCCGGAATTGAGAGTACAAAGAGCTACATGTTCTTGGGCACTCAAAGGTTCCAAGTTGCCTGAAATTCGGGATCCTGAGGCAGAGGGGACTGAATATGAGGGGGGTTCGGCCACCAGGGTTTGTCCATTCACTTACTGCTCACTTCATGGTCATCGCAATGCCAATGTACCCCCACTCAAGCGCCTGATTTCGATAAGGAGGCGCATGTTGAGGGCTCAGAGAGGCGTAACACAGGCAACTCAACCGTTGGAGAGAGTGAAAAGGTCTGGCAAAAATAAGGATGATCAGACAAATCACATGGCCTGCAATGGAAATAGCGTAGTTCATGAAACGAATGCTATGGTGTCACTCCCTGCACCGGATGGTTTAAGTCTGGAATTTTGTTGCATCGCTACAAATTTGGAGTCCACCAAAACAGACTGGAACGAAGAGAAATTCGCAGCGTCTGATCACAATGAAGGCTCTCTATCCACTTGCACAGATGATATGAGCGTTAATGATCCTACATTGAATGGAAAAAATTTGGAGGTTGATGATTGCGCAACTGTCAAACTACCAGATAATTTGGAAAGACCAACCCCTCATGATGTTTTGGAATTCACAAGGGACAATGAAAAAAAGGAAGTCTCTTCTGCTTCTCATAGTCCAGCTTTGAAGGAACAAATTGCAGGTAATGAGAACCAAAATAGAAGTGTGCAAGCAAGGGATCAGAAATATACCCGAATGTGGCAGTTGATGTACAAGCACGCAGCAAAAGGTATCACTCCAAATGTTGAAAACAAGCTCCCTCCTGATGGATCAGACAAGGAAGAGAAACCAAAAGATGCCAACATCACGTGTGACACAGACAATTTATGTTTCGCAGAAACAGATGAGCATACAGCTTCTGTAAATCACAGAGCAGGAGATGAAACTACTGAGGTATGTTGCCATGATGCCATTCAGCTTGTGCAAGAAGCATTTGATAACATTCTTCTCCCCGAAATTCAGGACTGCTCATATGATGATCAATCATCTACTAGTGGCGCCAGCTCAGACCGGGAAGCATTAGAACAGACAATAGATGAGGGTGGGGAGCAGAGCACCTCCACTACCAGTCATTTGTCTGAGACTAGTACGGCACAAAATCCAGAAGAGAAAACAGTGTCAGAAGTGGGAGTTAAGACGGACAAGAAAACACCCAAGAGCTGGAGCAGTCTTAAAAAATTCATTCTCCTCAAGAGATTTGTCAAGGCAGTTGAAAAGGTGAGGAACCTCAATTACGCGAAGCGACAGTACCTGCCTTTGCATCCTGATTTGGAAGCAGAAAAAATCAATCTAAGACAGCAGAAGACAGAAGAGAGAAAGAATGCCGAGGAGTGGATGCTTGATTATGCACTTCAACAGGTTATATCTAAACTACCTCAGCCTCAACAAAGAAGAGTAGCATTGCTGGTAGAAGCATTTGAAACAGTTCTCCCGTTTTCAGAAGTTAAGAGCAGCCAGAGGTCCAACGAAACAGATTCCACTAAATCAGATCTTCAAGTCTGCAATGGTCTGTCAGTTCAAAGTTCTGTTCATCAAGGTAAGGAGAGTGACTCGGAATCTTCTGCTGAAATCTTGCTTGGGAATGTGTCGTGTCCTGAGAAAAGTTTAAATGAGTCTCCCGATCAAGTCAGTGATTTTCAAATGCAAGAGCAGCGAAGTCAAGTGAATTTTCCTAAGCTTCAAGAGTCGAGTATAGGCCACTGTTCCATTCAAACCGAAAGGGATATTACTGTTCCTGAGGCCACTGGTGAAGCTCAGAAGGAGAATCAAATTGTTTCCATAAATGCTTGTGATCGGGATGAGAAAGTCATTGACTTTACCAGTATTTCTTCATTAGAAATCAAAGATCCCAAGTCATGTGACAAAACCTCCTTGGAGCGAGATGAACATGGTAGCCCCAGTTGTGTAGGTCTGGTAAATGAAGTGGTTCAAGAAGTCTCCAAAGAAGTTACTTTGATCACGAGTTTGGAACTTAGCAACATTGGTTCCATGGTGGAAAATATCGAATCTGACACCAATAAGTTAATCACCGAAACTGATGAACAGTCAAATTCATCTGAGGACCAAATCACTGAAAGTCACGAGGACTCAACAGCGGATAACACTGTGGTTTGTTTAGCTTCCATAGGATCAACTGAGGAGCAAATGGCAGCAAGAGAAGAGCTGAGCGGAATAGCAGATGAAGCCCGACGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

960

Amino Acids

106.24

Weight (kDa)

5.43

Isoelectric Point (pI)

56.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 605 - 705 1.6e-28 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1195
Acc36I ACCTGC 2 cut(s) 1313, 1941
AccB1I GGYRCC 1 cut(s) 1679
AccB7I CCANNNNNTGG 1 cut(s) 1842
AccBSI CCGCTC 1 cut(s) 2850
AccII CGCG 1 cut(s) 1919
AccIII TCCGGA 1 cut(s) 655
AciI CCGC 3 cut(s) 319, 2777, 2850
AclWI GGATC 8 cut(s) 228, 719, 732, 1148, 1368, 1459, 2522, 2818
AcoI YGGCCR 1 cut(s) 762
AcvI CACGTG 1 cut(s) 1491
AcyI GRCGYC 1 cut(s) 1680
AfaI GTAC 7 cut(s) 280, 517, 669, 822, 1394, 1771, 1931
AfiI CCNNNNNNNGG 5 cut(s) 1012, 1380, 1448, 1842, 2013
AflIII ACRYGT 2 cut(s) 680, 1490
AhlI ACTAGT 3 cut(s) 518, 1673, 1766
AjnI CCWGG 2 cut(s) 574, 767
AjuI GAANNNNNNNTTGG 2 cut(s) 2241, 2273
Alw21I GWGCWC 3 cut(s) 133, 398, 1739
Alw26I GTCTC 6 cut(s) 228, 521, 1285, 1757, 2301, 2623
AlwI GGATC 8 cut(s) 228, 719, 732, 1148, 1368, 1459, 2522, 2818
AlwNI CAGNNNCTG 4 cut(s) 461, 1100, 1541, 1933
Ama87I CYCGRG 1 cut(s) 397
Aor13HI TCCGGA 1 cut(s) 655
AoxI GGCC 4 cut(s) 762, 954, 2377, 2421
ApeKI GCWGC 8 cut(s) 26, 145, 148, 1094, 1403, 2133, 2332, 2831
ArsI GACNNNNNNTTYG 2 cut(s) 1043, 1075
Asp700I GAANNNNTTC 3 cut(s) 645, 2106, 2607
AspLEI GCGC 4 cut(s) 837, 858, 1196, 1682
AspS9I GGNCC 2 cut(s) 2142, 2743
AsuC2I CCSGG 1 cut(s) 1694
AsuHPI GGTGA 8 cut(s) 198, 233, 486, 579, 594, 1912, 2441, 2701
AvaI CYCGRG 1 cut(s) 397
AvaII GGWCC 2 cut(s) 2142, 2743
AxyI CCTNAGG 2 cut(s) 729, 2418
BaeGI GKGCMC 1 cut(s) 694
BamHI GGATCC 1 cut(s) 724
BanI GGYRCC 1 cut(s) 1679
BanII GRGCYC 1 cut(s) 871
BarI GAAGNNNNNNTAC 2 cut(s) 1913, 1945
BauI CACGAG 2 cut(s) 2641, 2762
BbrPI CACGTG 1 cut(s) 1491
BbsI GAAGAC 1 cut(s) 1991
Bbv12I GWGCWC 3 cut(s) 133, 398, 1739
BbvCI CCTCAGC 1 cut(s) 2058
BbvI GCAGC 8 cut(s) 38, 135, 157, 1106, 1415, 2145, 2344, 2843
BccI CCATC 3 cut(s) 473, 1010, 1442
BceAI ACGGC 3 cut(s) 124, 205, 1788
BciT130I CCWGG 2 cut(s) 576, 769
BclI TGATCA 4 cut(s) 937, 1102, 1660, 2637
BcnI CCSGG 1 cut(s) 1694
BcoDI GTCTC 6 cut(s) 228, 521, 1285, 1757, 2301, 2623
BcuI ACTAGT 3 cut(s) 518, 1673, 1766
BfaI CTAG 5 cut(s) 324, 519, 531, 1674, 1767
BfmI CTRYAG 2 cut(s) 143, 453
BfoI RGCGCY 2 cut(s) 838, 1683
BfuAI ACCTGC 2 cut(s) 1313, 1941
BglII AGATCT 1 cut(s) 2170
BisI GCNGC 8 cut(s) 27, 146, 149, 1095, 1404, 2134, 2333, 2832
BlpI GCTNAGC 1 cut(s) 2846
BlsI GCNGC 8 cut(s) 28, 147, 150, 1096, 1405, 2135, 2334, 2833
BmcAI AGTACT 1 cut(s) 517
Bme1390I CCNGG 3 cut(s) 576, 769, 1694
Bme18I GGWCC 2 cut(s) 2142, 2743
BmeT110I CYCGRG 1 cut(s) 397
BmgT120I GGNCC 2 cut(s) 2142, 2743
BmiI GGNNCC 8 cut(s) 40, 406, 632, 703, 726, 1681, 1907, 2668
BmrFI CCNGG 3 cut(s) 576, 769, 1694
BmrI ACTGGG 1 cut(s) 2574
BmsI GCATC 6 cut(s) 469, 1050, 1468, 1579, 1951, 2007
BmuI ACTGGG 1 cut(s) 2574
BpiI GAAGAC 1 cut(s) 1991
BplI GAGNNNNNCTC 2 cut(s) 2216, 2248
BpmI CTGGAG 1 cut(s) 1864
Bpu10I CCTNAGC 2 cut(s) 2058, 2355
Bpu1102I GCTNAGC 1 cut(s) 2846
BpuEI CTTGAG 2 cut(s) 815, 1856
BpuMI CCSGG 1 cut(s) 1694
Bsa29I ATCGAT 1 cut(s) 188
BsaAI YACGTR 1 cut(s) 1491
BsaHI GRCGYC 1 cut(s) 1680
BsaJI CCNNGG 4 cut(s) 768, 2007, 2553, 2670
BsaWI WCCGGW 2 cut(s) 655, 1012
BsaXI ACNNNNNCTCC 2 cut(s) 2534, 2564
Bsc4I CCNNNNNNNGG 5 cut(s) 1012, 1380, 1448, 1842, 2013
Bse1I ACTGG 7 cut(s) 360, 507, 1079, 1749, 2431, 2502, 2580
Bse21I CCTNAGG 2 cut(s) 729, 2418
Bse3DI GCAATG 4 cut(s) 817, 967, 2081, 2191
BseAI TCCGGA 1 cut(s) 655
BseBI CCWGG 2 cut(s) 576, 769
BseCI ATCGAT 1 cut(s) 188
BseDI CCNNGG 4 cut(s) 768, 2007, 2553, 2670
BseGI GGATG 8 cut(s) 10, 251, 470, 940, 1021, 1942, 2022, 2485
BseLI CCNNNNNNNGG 5 cut(s) 1012, 1380, 1448, 1842, 2013
BseMI GCAATG 4 cut(s) 817, 967, 2081, 2191
BseNI ACTGG 7 cut(s) 360, 507, 1079, 1749, 2431, 2502, 2580
BseRI GAGGAG 4 cut(s) 561, 1859, 2024, 2834
BseSI GKGCMC 1 cut(s) 694
BseXI GCAGC 8 cut(s) 38, 135, 157, 1106, 1415, 2145, 2344, 2843
BsgI GTGCAG 1 cut(s) 993
Bsh1236I CGCG 1 cut(s) 1919
BshFI GGCC 4 cut(s) 764, 956, 2379, 2423
BshNI GGYRCC 1 cut(s) 1679
BshVI ATCGAT 1 cut(s) 188
BsiHKAI GWGCWC 3 cut(s) 133, 398, 1739
BsiHKCI CYCGRG 1 cut(s) 397
BsiSI CCGG 3 cut(s) 656, 1013, 1693
BslFI GGGAC 2 cut(s) 754, 1274
BslI CCNNNNNNNGG 5 cut(s) 1012, 1380, 1448, 1842, 2013
BsmAI GTCTC 6 cut(s) 228, 521, 1285, 1757, 2301, 2623
BsmBI CGTCTC 1 cut(s) 228
BsmFI GGGAC 2 cut(s) 754, 1274
BsmI GAATGC 2 cut(s) 994, 2008
BsnI GGCC 4 cut(s) 764, 956, 2379, 2423
BsoBI CYCGRG 1 cut(s) 397
Bsp1286I GDGCHC 5 cut(s) 133, 398, 694, 871, 1739
Bsp13I TCCGGA 1 cut(s) 655
Bsp1407I TGTACA 1 cut(s) 1392
Bsp1720I GCTNAGC 1 cut(s) 2846
Bsp19I CCATGG 1 cut(s) 2670
BspACI CCGC 3 cut(s) 319, 2777, 2850
BspANI GGCC 4 cut(s) 764, 956, 2379, 2423
BspDI ATCGAT 1 cut(s) 188
BspEI TCCGGA 1 cut(s) 655
BspFNI CGCG 1 cut(s) 1919
BspHI TCATGA 2 cut(s) 979, 1237
BspLI GGNNCC 8 cut(s) 40, 406, 632, 703, 726, 1681, 1907, 2668
BspMAI CTGCAG 2 cut(s) 147, 457
BspMI ACCTGC 2 cut(s) 1313, 1941
BspPI GGATC 8 cut(s) 228, 719, 732, 1148, 1368, 1459, 2522, 2818
BspQI GCTCTTC 1 cut(s) 2835
BspT107I GGYRCC 1 cut(s) 1679
BsrBI CCGCTC 1 cut(s) 2850
BsrDI GCAATG 4 cut(s) 817, 967, 2081, 2191
BsrGI TGTACA 1 cut(s) 1392
BsrI ACTGG 7 cut(s) 360, 507, 1079, 1749, 2431, 2502, 2580
BssECI CCNNGG 4 cut(s) 768, 2007, 2553, 2670
BssNI GRCGYC 1 cut(s) 1680
BssSI CACGAG 2 cut(s) 2641, 2762
BssT1I CCWWGG 2 cut(s) 2553, 2670
Bst2BI CACGAG 2 cut(s) 2641, 2762
Bst2UI CCWGG 2 cut(s) 576, 769
Bst4CI ACNGT 9 cut(s) 900, 1201, 1798, 1929, 2107, 2384, 2413, 2727, 2788
Bst6I CTCTTC 8 cut(s) 155, 1077, 1288, 1458, 1782, 1986, 2068, 2835
BstACI GRCGYC 1 cut(s) 1680
BstAPI GCANNNNNTGC 1 cut(s) 461
BstAUI TGTACA 1 cut(s) 1392
BstBAI YACGTR 1 cut(s) 1491
BstC8I GCNNGC 6 cut(s) 336, 453, 958, 1353, 1401, 1684
BstDSI CCRYGG 1 cut(s) 2670
BstF5I GGATG 8 cut(s) 10, 251, 470, 940, 1021, 1942, 2022, 2485
BstFNI CGCG 1 cut(s) 1919
BstH2I RGCGCY 2 cut(s) 838, 1683
BstHHI GCGC 4 cut(s) 837, 858, 1196, 1682
BstMAI GTCTC 6 cut(s) 228, 521, 1285, 1757, 2301, 2623
BstMWI GCNNNNNNNGC 4 cut(s) 145, 461, 2332, 2864
BstNI CCWGG 2 cut(s) 576, 769
BstNSI RCATGY 2 cut(s) 684, 862
BstSCI CCNGG 3 cut(s) 574, 767, 1692
BstSFI CTRYAG 2 cut(s) 143, 453
BstSLI GKGCMC 1 cut(s) 694
BstUI CGCG 1 cut(s) 1919
BstV1I GCAGC 8 cut(s) 38, 135, 157, 1106, 1415, 2145, 2344, 2843
BstV2I GAAGAC 1 cut(s) 1991
BstX2I RGATCY 4 cut(s) 233, 724, 2170, 2527
BstYI RGATCY 4 cut(s) 233, 724, 2170, 2527
Bsu15I ATCGAT 1 cut(s) 188
Bsu36I CCTNAGG 2 cut(s) 729, 2418
BsuRI GGCC 4 cut(s) 764, 956, 2379, 2423
BsuTUI ATCGAT 1 cut(s) 188
BtgI CCRYGG 1 cut(s) 2670
BtgZI GCGATG 2 cut(s) 791, 1027
BtsCI GGATG 8 cut(s) 10, 251, 470, 940, 1021, 1942, 2022, 2485
BtsIMutI CAGTG 9 cut(s) 255, 474, 587, 1803, 2317, 2380, 2424, 2751, 2784
BveI ACCTGC 2 cut(s) 1313, 1941
Cac8I GCNNGC 6 cut(s) 336, 453, 958, 1353, 1401, 1684
CaiI CAGNNNCTG 4 cut(s) 461, 1100, 1541, 1933
CciI TCATGA 2 cut(s) 979, 1237
CfoI GCGC 4 cut(s) 837, 858, 1196, 1682
Cfr13I GGNCC 2 cut(s) 2142, 2743
ClaI ATCGAT 1 cut(s) 188
CseI GACGC 2 cut(s) 89, 1086
Csp6I GTAC 7 cut(s) 279, 516, 668, 821, 1393, 1770, 1930
CviQI GTAC 7 cut(s) 279, 516, 668, 821, 1393, 1770, 1930
DinI GGCGCC 1 cut(s) 1681
DraI TTTAAA 2 cut(s) 366, 2289
EaeI YGGCCR 1 cut(s) 762
Eam1104I CTCTTC 8 cut(s) 155, 1077, 1288, 1458, 1782, 1986, 2068, 2835
EarI CTCTTC 8 cut(s) 155, 1077, 1288, 1458, 1782, 1986, 2068, 2835
Eco130I CCWWGG 2 cut(s) 2553, 2670
Eco24I GRGCYC 1 cut(s) 871
Eco47I GGWCC 2 cut(s) 2142, 2743
Eco72I CACGTG 1 cut(s) 1491
Eco81I CCTNAGG 2 cut(s) 729, 2418
Eco88I CYCGRG 1 cut(s) 397
EcoRI GAATTC 2 cut(s) 295, 1250
EcoRII CCWGG 2 cut(s) 574, 767
EcoT14I CCWWGG 2 cut(s) 2553, 2670
EcoT38I GRGCYC 1 cut(s) 871
EgeI GGCGCC 1 cut(s) 1681
EheI GGCGCC 1 cut(s) 1681
ErhI CCWWGG 2 cut(s) 2553, 2670
Esp3I CGTCTC 1 cut(s) 228
FaqI GGGAC 2 cut(s) 754, 1274
FauNDI CATATG 2 cut(s) 411, 1654
FbaI TGATCA 4 cut(s) 937, 1102, 1660, 2637
Fnu4HI GCNGC 8 cut(s) 27, 146, 149, 1095, 1404, 2134, 2333, 2832
FokI GGATG 8 cut(s) 17, 238, 457, 947, 1028, 1929, 2029, 2492
FriOI GRGCYC 1 cut(s) 871
Fsp4HI GCNGC 8 cut(s) 27, 146, 149, 1095, 1404, 2134, 2333, 2832
FspBI CTAG 5 cut(s) 324, 519, 531, 1674, 1767
FspI TGCGCA 1 cut(s) 1195
GlaI GCGC 4 cut(s) 836, 857, 1195, 1681
GluI GCNGC 8 cut(s) 27, 146, 149, 1095, 1404, 2134, 2333, 2832
GsuI CTGGAG 1 cut(s) 1864
HaeII RGCGCY 2 cut(s) 838, 1683
HaeIII GGCC 4 cut(s) 764, 956, 2379, 2423
HapII CCGG 3 cut(s) 656, 1013, 1693
HgaI GACGC 2 cut(s) 89, 1086
HhaI GCGC 4 cut(s) 837, 858, 1196, 1682
Hin1I GRCGYC 1 cut(s) 1680
Hin6I GCGC 4 cut(s) 835, 856, 1194, 1680
HinP1I GCGC 4 cut(s) 835, 856, 1194, 1680
HincII GTYRAC 2 cut(s) 66, 183
HindII GTYRAC 2 cut(s) 66, 183
HindIII AAGCTT 1 cut(s) 2357
HpaII CCGG 3 cut(s) 656, 1013, 1693
HphI GGTGA 8 cut(s) 198, 233, 486, 579, 594, 1912, 2441, 2701
Hpy166II GTNNAC 4 cut(s) 66, 183, 587, 1062
Hpy8I GTNNAC 4 cut(s) 66, 183, 587, 1062
Hpy99I CGWCG 1 cut(s) 2874
HpyAV CCTTC 4 cut(s) 96, 1106, 1303, 2434
HpyCH4III ACNGT 9 cut(s) 900, 1201, 1798, 1929, 2107, 2384, 2413, 2727, 2788
HpyCH4IV ACGT 1 cut(s) 1490
HpyF10VI GCNNNNNNNGC 4 cut(s) 145, 461, 2332, 2864
HpySE526I ACGT 1 cut(s) 1490
Hsp92I GRCGYC 1 cut(s) 1680
HspAI GCGC 4 cut(s) 835, 856, 1194, 1680
KasI GGCGCC 1 cut(s) 1679
Kpn2I TCCGGA 1 cut(s) 655
Ksp22I TGATCA 4 cut(s) 937, 1102, 1660, 2637
LguI GCTCTTC 1 cut(s) 2835
LmnI GCTCC 7 cut(s) 13, 128, 1443, 1729, 1845, 2557, 2821
Lsp1109I GCAGC 8 cut(s) 38, 135, 157, 1106, 1415, 2145, 2344, 2843
LweI GCATC 6 cut(s) 469, 1050, 1468, 1579, 1951, 2007
MaeI CTAG 5 cut(s) 324, 519, 531, 1674, 1767
MaeII ACGT 1 cut(s) 1490
MaeIII GTNAC 7 cut(s) 880, 999, 1493, 2228, 2540, 2629, 2759
MbiI CCGCTC 1 cut(s) 2850
MflI RGATCY 4 cut(s) 233, 724, 2170, 2527
MhlI GDGCHC 5 cut(s) 133, 398, 694, 871, 1739
Mly113I GGCGCC 1 cut(s) 1680
MlyI GAGTC 5 cut(s) 1067, 2225, 2303, 2375, 2762
MmeI TCCRAC 4 cut(s) 66, 636, 882, 2169
MroI TCCGGA 1 cut(s) 655
MroXI GAANNNNTTC 3 cut(s) 645, 2106, 2607
MseI TTAA 9 cut(s) 330, 365, 1022, 1149, 1815, 1854, 2127, 2288, 2705
MslI CAYNNNNRTG 1 cut(s) 810
MspA1I CMGCKG 3 cut(s) 29, 148, 2777
MspI CCGG 3 cut(s) 656, 1013, 1693
MspR9I CCNGG 3 cut(s) 576, 769, 1694
Mva1269I GAATGC 2 cut(s) 994, 2008
MvaI CCWGG 2 cut(s) 576, 769
MvnI CGCG 1 cut(s) 1919
MwoI GCNNNNNNNGC 4 cut(s) 145, 461, 2332, 2864
NarI GGCGCC 1 cut(s) 1680
NciI CCSGG 1 cut(s) 1694
NcoI CCATGG 1 cut(s) 2670
NdeI CATATG 2 cut(s) 411, 1654
NlaIV GGNNCC 8 cut(s) 40, 406, 632, 703, 726, 1681, 1907, 2668
NmeAIII GCCGAG 1 cut(s) 2032
NmuCI GTSAC 5 cut(s) 999, 1493, 2228, 2540, 2759
NsbI TGCGCA 1 cut(s) 1195
NspI RCATGY 2 cut(s) 684, 862
PagI TCATGA 2 cut(s) 979, 1237
PciI ACATGT 1 cut(s) 680
PciSI GCTCTTC 1 cut(s) 2835
PctI GAATGC 2 cut(s) 994, 2008
PdmI GAANNNNTTC 3 cut(s) 645, 2106, 2607
PfeI GAWTC 8 cut(s) 263, 343, 458, 485, 2156, 2237, 2446, 2687
PflMI CCANNNNNTGG 1 cut(s) 1842
PkrI GCNGC 8 cut(s) 28, 147, 150, 1096, 1405, 2135, 2334, 2833
PleI GAGTC 5 cut(s) 1066, 2225, 2302, 2374, 2762
PluTI GGCGCC 1 cut(s) 1683
PmaCI CACGTG 1 cut(s) 1491
PmlI CACGTG 1 cut(s) 1491
PpsI GAGTC 5 cut(s) 1066, 2225, 2302, 2374, 2762
Ppu21I YACGTR 1 cut(s) 1491
PscI ACATGT 1 cut(s) 680
Psp6I CCWGG 2 cut(s) 574, 767
PspCI CACGTG 1 cut(s) 1491
PspGI CCWGG 2 cut(s) 574, 767
PspN4I GGNNCC 8 cut(s) 40, 406, 632, 703, 726, 1681, 1907, 2668
PspPI GGNCC 2 cut(s) 2142, 2743
PsrI GAACNNNNNNTAC 2 cut(s) 1898, 1930
PstI CTGCAG 2 cut(s) 147, 457
PstNI CAGNNNCTG 4 cut(s) 461, 1100, 1541, 1933
PsuI RGATCY 4 cut(s) 233, 724, 2170, 2527
PvuII CAGCTG 2 cut(s) 29, 148
RsaI GTAC 7 cut(s) 280, 517, 669, 822, 1394, 1771, 1931
RsaNI GTAC 7 cut(s) 279, 516, 668, 821, 1393, 1770, 1930
RseI CAYNNNNRTG 1 cut(s) 810
SapI GCTCTTC 1 cut(s) 2835
SaqAI TTAA 9 cut(s) 330, 365, 1022, 1149, 1815, 1854, 2127, 2288, 2705
SatI GCNGC 8 cut(s) 27, 146, 149, 1095, 1404, 2134, 2333, 2832
Sau96I GGNCC 2 cut(s) 2142, 2743
ScaI AGTACT 1 cut(s) 517
SchI GAGTC 5 cut(s) 1067, 2225, 2303, 2375, 2762
ScrFI CCNGG 3 cut(s) 576, 769, 1694
SduI GDGCHC 5 cut(s) 133, 398, 694, 871, 1739
SfaNI GCATC 6 cut(s) 469, 1050, 1468, 1579, 1951, 2007
SfcI CTRYAG 2 cut(s) 143, 453
SfoI GGCGCC 1 cut(s) 1681
SinI GGWCC 2 cut(s) 2142, 2743
SmiMI CAYNNNNRTG 1 cut(s) 810
SmlI CTYRAG 2 cut(s) 830, 1871
SmoI CTYRAG 2 cut(s) 830, 1871
SpeI ACTAGT 3 cut(s) 518, 1673, 1766
SsiI CCGC 3 cut(s) 319, 2777, 2850
SspDI GGCGCC 1 cut(s) 1679
SspI AATATT 1 cut(s) 421
SspMI CTAG 5 cut(s) 324, 519, 531, 1674, 1767
StyD4I CCNGG 3 cut(s) 574, 767, 1692
StyI CCWWGG 2 cut(s) 2553, 2670
TaaI ACNGT 9 cut(s) 900, 1201, 1798, 1929, 2107, 2384, 2413, 2727, 2788
TaiI ACGT 1 cut(s) 1493
TaqI TCGA 6 cut(s) 188, 226, 563, 845, 2369, 2685
TatI WGTACW 4 cut(s) 278, 515, 667, 1392
TfiI GAWTC 8 cut(s) 263, 343, 458, 485, 2156, 2237, 2446, 2687
Tru1I TTAA 9 cut(s) 330, 365, 1022, 1149, 1815, 1854, 2127, 2288, 2705
Tru9I TTAA 9 cut(s) 330, 365, 1022, 1149, 1815, 1854, 2127, 2288, 2705
TscAI CASTG 9 cut(s) 262, 481, 587, 1803, 2317, 2387, 2431, 2758, 2791
TseFI GTSAC 5 cut(s) 999, 1493, 2228, 2540, 2759
TseI GCWGC 8 cut(s) 26, 145, 148, 1094, 1403, 2133, 2332, 2831
Tsp45I GTSAC 5 cut(s) 999, 1493, 2228, 2540, 2759
TspGWI ACGGA 1 cut(s) 1835
TspRI CASTG 9 cut(s) 262, 481, 587, 1803, 2317, 2387, 2431, 2758, 2791
Van91I CCANNNNNTGG 1 cut(s) 1842
VpaK11BI GGWCC 2 cut(s) 2142, 2743
XceI RCATGY 2 cut(s) 684, 862
XmnI GAANNNNTTC 3 cut(s) 645, 2106, 2607
XspI CTAG 5 cut(s) 324, 519, 531, 1674, 1767
ZrmI AGTACT 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.