Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0005134

Physical Location & Seq

Genomic Coordinates Reverse (-)

11090710 .. 11095710

Gene Structure 5001 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1089200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2052 bp

ATGGGAAGGAGATGTAGGGTTTGGTGGCCAAAGCAACTCACATTGAGCAAACCATCATCATCCTCCAATTTCTTGCTTGGTTGGTTTGTTTCTTCTTCTCCGTCTTCTCTTGACGTCGTCGTCGCTTTCGCCTGCACTGAACTCGCGCTTTCTGGTATTCAATCAAGTCTCCAGGGAATTCTTTGCGACGCGAACAGAAGGATGCCTGTGTTACTACAGGATAATTCTATGTTGTGTGTAGTGGGTCATTTGTTTAAGGTTCAAACAGAAGAGGATCAATCTCATTCTTCTTCTTGTGGGTGCCACAAGCTCAGTGGATCAGTAGAACAATGTAGGGAAAATTTTGAGGGAAACGGTTATTGGATTACGATGATGTGTGATCCTCAAGAACAAGTTGGAAAGGACATTACTTGGATTCCGAAACTGCATCACATTCACTGGCATGGGCAAATCGTTTCTCGGTGTGATGTCCACCTAATTTTGTATGAGACTCCCGCCTATGGTGCCCACCATTTCTCATTACATCCTTGGAATTCATTTGAGAAAGTGAGTGCACCTTTGAGAAAACCCAAGTGGGTTGATGAACTTCACCAGAAGCAGCAACTCCTTGATTTGGATACAGTCATTCTGGCAATTAATAGCTCCGCAACCGCTGAAATTGTTTTTGAGAGCTGCCTAGGTCCCAAGACGTCTTTTGTGTGCTTTTCTACTGTTTACATGTTTGTGGGCTTTGTATGGCAACTATTCGCCATGTCTGTGGCTTCATTATCCGCTTTATTCTATGTCGTTCTTCAGTTTCTGTATAGACTTCTGAATTATGCATCAGACTCTTGGATAGACCTTATATTAGTAAAGGTATTCAGCATTTCAAGGATAAATGTCAGAATCCGTGGTTCTCAGATCTTGTATTGGCCAATCGTTCTTCAAGATAATGGCATGAGGTCACCATCAAGTGTGGAATACGCGGAGAAAGCCGCATTGCATAAGCATTCCGTGTGGTCAAGTTTAGCTGTTGATGTACTTCTGGGAAACTTGTTTGGTTTGGTACTGTTGTATCATGCAGAATCTGTTTGCATATGGATCTTGAAATTTTCCAGTGACATGACGAATGAATTATTGCGCTCAGGCTGTGTGTGGTTGATGGGGGTACCTGCAGGTTTTAAGTTAAACAATGAATTGGCAGGAGTTCTTGGGATGATTTCTCTAACTGCTATCCAGATTTGGTCTACCATTTGGATCTTTTTGGGGTCCCACTTTCTTTATTTCATTAGAGGACTTGCTATATCAGGAATCATTTTTGGGGTGACTATACCTGCTGCTTTGATAATCGACTTGATTGCACTAGCAACGTTGCATGTGTCTACTCTTCATTGGTTGATATCACTTTTGTATTCAACTCAGATACAGGCATTAGCAGCTCTGTGGCGCCTTTTCAGGGGCCGAAAGTGGAATCCTCTTCGTCAGAGATTAGATAGCTATGACTACACTGTCAAGCAACACATCGTTGGATCTCTTCTGTTCACACCACTCCTACTTCTATTACCGACAACTTCTGTTTTCTACATCTTTTTTACCATTATGAACACAACTATCACCCTTATCTGTATACTGATTGAAGTGACCATATCTGTTATCCATGCCACACCATATATCAAAATTTTCCTTTGGTTGGTCAGGCCGAGAAGATTTCCAACTGGGATATGGTTTGAAATAATGTCTGTTTGGAGTGGTGGAATTGATTCTCCTAAGGGTATTATTTCACCATCAGATAAACTTCAGACCGGAAAGGACCTCACTAGATCTTCTGTTGTGGTTTCATTTCTCCATAGTAACTTCTTGACTGTAGGACAAATAGTCATGCCTCACTACAAAAAGCTTCTTTCTGGGAAACCTCGGACATTGGTTGCTACTGCAGCTTATGGAGTCCTCACTGGCAGACGGATTCCATCTACAATCGGAACTGATCTTCCAATATTTCCGTGGATGCTAATCTCCTACAAAGAGTATTGGCGTCTCTGCCGCGATTCAGTTCTCGCATGCCACAGAACGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000506 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006497 GO:0006505 GO:0006506 GO:0006629 GO:0006643 GO:0006644 GO:0006650 GO:0006661 GO:0006664 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009059 GO:0009247 GO:0009987 GO:0012505 GO:0016020 GO:0019538 GO:0019637 GO:0031984 GO:0032991 GO:0034645 GO:0036211 GO:0042157 GO:0042158 GO:0042175 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046467 GO:0046474 GO:0046486 GO:0046488 GO:0071704 GO:0090407 GO:0098796 GO:0098827 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1902494 GO:1903509 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00563 ko01100 map00563 map01100

Pfam Domains

Protein Families

Gpi1

Protein Analysis

684

Amino Acids

77.13

Weight (kDa)

8.99

Isoelectric Point (pI)

44.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gpi1	PF05024	293 - 564	6.2e-80	N-acetylglucosaminyl transferase component (Gpi1)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005134)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57170 AT3G57170
fragaria_vesca	FvH4_6g24900 FvH4_6g24900 FvH4_6g24900 FvH4_6g24900 FvH4_6g24900 FvH4_6g24900 FvH4_6g24900 FvH4_6g24900
malus_domestica	MD07G1151600.v1.1 MD12G1089200.v1.1 MD14G1081800.v1.1
prunus_persica	Prupe.4G274500_v2.0.a1 Prupe.5G000500_v2.0.a1
pyrus_communis	pycom07g15000 pycom12g07440 pycom14g07110
rosa_chinensis	RchiOBHm_Chr2g0124601
rosa_laevigata	RLG00000018807
rosa_multiflora	Rmu_sc0042054.1_g000001
rosa_roxburghii	Rroxscaffold_2G00119850
rosa_rugosa	Rorug02G0251500 Rorug02G0251500
rosa_samantha	Rh2AG310200 Rh2BG318700 Rh2CG297100 Rh2CG302000 Rh2DG333900
rosa_wichuraiana	Rw2G025090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	119, 1487
AatII	GACGTC	2 cut(s)	117, 692
Acc36I	ACCTGC	3 cut(s)	1145, 1159, 1321
Acc65I	GGTACC	1 cut(s)	1147
AccB1I	GGYRCC	4 cut(s)	300, 503, 1147, 1425
AccI	GTMKAC	3 cut(s)	1226, 1361, 1606
AccII	CGCG	4 cut(s)	146, 191, 965, 2022
AciI	CCGC	7 cut(s)	495, 645, 651, 771, 965, 975, 2020
AclI	AACGTT	1 cut(s)	1349
AclWI	GGATC	6 cut(s)	282, 325, 374, 1088, 1244, 1516
AcoI	YGGCCR	2 cut(s)	26, 911
AcsI	RAATTY	5 cut(s)	177, 340, 532, 1088, 1656
AcuI	CTGAAG	2 cut(s)	776, 1760
AcyI	GRCGYC	4 cut(s)	114, 689, 1426, 2011
AfaI	GTAC	3 cut(s)	1020, 1047, 1149
AfiI	CCNNNNNNNGG	4 cut(s)	500, 613, 1435, 1669
AflIII	ACRYGT	1 cut(s)	717
AgsI	TTSAA	8 cut(s)	161, 263, 870, 926, 1087, 1395, 1616, 1709
AhdI	GACNNNNNGTC	1 cut(s)	1853
AjnI	CCWGG	1 cut(s)	171
AjuI	GAANNNNNNNTTGG	2 cut(s)	343, 375
AloI	GAACNNNNNNTCC	2 cut(s)	1951, 1983
AluBI	AGCT	8 cut(s)	310, 642, 672, 1010, 1418, 1476, 1876, 1916
AluI	AGCT	8 cut(s)	310, 642, 672, 1010, 1418, 1476, 1876, 1916
Alw21I	GWGCWC	1 cut(s)	556
Alw26I	GTCTC	3 cut(s)	173, 482, 2018
Alw44I	GTGCAC	1 cut(s)	552
AlwI	GGATC	6 cut(s)	282, 325, 374, 1088, 1244, 1516
AlwNI	CAGNNNCTG	2 cut(s)	799, 1067
AoxI	GGCC	4 cut(s)	26, 911, 1438, 1676
ApaLI	GTGCAC	1 cut(s)	552
ApeKI	GCWGC	5 cut(s)	598, 672, 1316, 1415, 1913
ApoI	RAATTY	5 cut(s)	177, 340, 532, 1088, 1656
AseI	ATTAAT	1 cut(s)	636
Asp700I	GAANNNNTTC	1 cut(s)	1738
Asp718I	GGTACC	1 cut(s)	1147
AspA2I	CCTAGG	1 cut(s)	676
AspLEI	GCGC	3 cut(s)	148, 1122, 1428
AspS9I	GGNCC	4 cut(s)	680, 1248, 1438, 1789
AsuHPI	GGTGA	5 cut(s)	581, 936, 1315, 1585, 1752
AvaII	GGWCC	3 cut(s)	680, 1248, 1789
AvrII	CCTAGG	1 cut(s)	676
AxyI	CCTNAGG	1 cut(s)	1746
BaeGI	GKGCMC	2 cut(s)	508, 556
BaeI	ACNNNNGTAYC	4 cut(s)	1037, 1037, 1070, 1070
BalI	TGGCCA	2 cut(s)	28, 913
BanI	GGYRCC	4 cut(s)	300, 503, 1147, 1425
BbsI	GAAGAC	1 cut(s)	96
Bbv12I	GWGCWC	1 cut(s)	556
BbvI	GCAGC	5 cut(s)	610, 659, 1303, 1427, 1925
BccI	CCATC	5 cut(s)	61, 955, 1135, 1771, 1954
BcgI	CGANNNNNNTGC	4 cut(s)	124, 158, 1319, 1353
BciT130I	CCWGG	1 cut(s)	173
BciVI	GTATCC	1 cut(s)	610
BcoDI	GTCTC	3 cut(s)	173, 482, 2018
BfaI	CTAG	3 cut(s)	677, 1343, 1797
BfmI	CTRYAG	4 cut(s)	215, 1152, 1842, 1911
BfoI	RGCGCY	1 cut(s)	1429
BfuAI	ACCTGC	3 cut(s)	1145, 1159, 1321
BfuI	GTATCC	1 cut(s)	610
BglII	AGATCT	2 cut(s)	900, 1799
BisI	GCNGC	7 cut(s)	599, 673, 975, 1317, 1416, 1914, 2020
BlnI	CCTAGG	1 cut(s)	676
BlsI	GCNGC	7 cut(s)	600, 674, 976, 1318, 1417, 1915, 2021
Bme1390I	CCNGG	1 cut(s)	173
Bme18I	GGWCC	3 cut(s)	680, 1248, 1789
BmeRI	GACNNNNNGTC	1 cut(s)	1853
BmgT120I	GGNCC	4 cut(s)	680, 1248, 1438, 1789
BmiI	GGNNCC	8 cut(s)	302, 505, 682, 1149, 1249, 1250, 1427, 1439
BmrFI	CCNGG	1 cut(s)	173
BmrI	ACTGGG	1 cut(s)	1704
BmsI	GCATC	4 cut(s)	192, 436, 830, 1974
BmuI	ACTGGG	1 cut(s)	1704
BpiI	GAAGAC	1 cut(s)	96
BpmI	CTGGAG	1 cut(s)	155
Bpu10I	CCTNAGC	1 cut(s)	1123
BpuEI	CTTGAG	1 cut(s)	369
BsaBI	GATNNNNATC	1 cut(s)	1988
BsaHI	GRCGYC	4 cut(s)	114, 689, 1426, 2011
BsaJI	CCNNGG	6 cut(s)	172, 527, 676, 889, 1892, 1979
BsaWI	WCCGGW	1 cut(s)	1781
Bsc4I	CCNNNNNNNGG	4 cut(s)	500, 613, 1435, 1669
Bse1I	ACTGG	4 cut(s)	443, 1095, 1699, 1936
Bse21I	CCTNAGG	1 cut(s)	1746
Bse3DI	GCAATG	1 cut(s)	977
Bse8I	GATNNNNATC	1 cut(s)	1988
BseBI	CCWGG	1 cut(s)	173
BseDI	CCNNGG	6 cut(s)	172, 527, 676, 889, 1892, 1979
BseGI	GGATG	5 cut(s)	59, 207, 523, 1200, 1989
BseJI	GATNNNNATC	1 cut(s)	1988
BseLI	CCNNNNNNNGG	4 cut(s)	500, 613, 1435, 1669
BseMI	GCAATG	1 cut(s)	977
BseMII	CTCAG	4 cut(s)	325, 911, 1137, 1412
BseNI	ACTGG	4 cut(s)	443, 1095, 1699, 1936
BseSI	GKGCMC	2 cut(s)	508, 556
BseXI	GCAGC	5 cut(s)	610, 659, 1303, 1427, 1925
BsgI	GTGCAG	1 cut(s)	118
Bsh1236I	CGCG	4 cut(s)	146, 191, 965, 2022
BshFI	GGCC	4 cut(s)	28, 913, 1440, 1678
BshNI	GGYRCC	4 cut(s)	300, 503, 1147, 1425
BsiHKAI	GWGCWC	1 cut(s)	556
BsiSI	CCGG	1 cut(s)	1782
BslFI	GGGAC	2 cut(s)	666, 1234
BslI	CCNNNNNNNGG	4 cut(s)	500, 613, 1435, 1669
BsmAI	GTCTC	3 cut(s)	173, 482, 2018
BsmBI	CGTCTC	1 cut(s)	2018
BsmFI	GGGAC	2 cut(s)	666, 1234
BsmI	GAATGC	1 cut(s)	988
BsnI	GGCC	4 cut(s)	28, 913, 1440, 1678
Bsp1286I	GDGCHC	2 cut(s)	508, 556
Bsp143I	GATC	9 cut(s)	274, 317, 379, 900, 1080, 1236, 1508, 1799, 1963
BspACI	CCGC	7 cut(s)	495, 645, 651, 771, 965, 975, 2020
BspANI	GGCC	4 cut(s)	28, 913, 1440, 1678
BspCNI	CTCAG	4 cut(s)	324, 910, 1136, 1411
BspFNI	CGCG	4 cut(s)	146, 191, 965, 2022
BspLI	GGNNCC	8 cut(s)	302, 505, 682, 1149, 1249, 1250, 1427, 1439
BspMAI	CTGCAG	2 cut(s)	1156, 1915
BspMI	ACCTGC	3 cut(s)	1145, 1159, 1321
BspPI	GGATC	6 cut(s)	282, 325, 374, 1088, 1244, 1516
BspT107I	GGYRCC	4 cut(s)	300, 503, 1147, 1425
BsrDI	GCAATG	1 cut(s)	977
BsrI	ACTGG	4 cut(s)	443, 1095, 1699, 1936
BssECI	CCNNGG	6 cut(s)	172, 527, 676, 889, 1892, 1979
BssMI	GATC	9 cut(s)	274, 317, 379, 900, 1080, 1236, 1508, 1799, 1963
BssNAI	GTATAC	1 cut(s)	1607
BssNI	GRCGYC	4 cut(s)	114, 689, 1426, 2011
BssT1I	CCWWGG	2 cut(s)	527, 676
Bst1107I	GTATAC	1 cut(s)	1607
Bst2UI	CCWGG	1 cut(s)	173
Bst4CI	ACNGT	6 cut(s)	356, 622, 712, 1050, 1489, 1843
Bst6I	CTCTTC	4 cut(s)	264, 1371, 1461, 1518
BstACI	GRCGYC	4 cut(s)	114, 689, 1426, 2011
BstC8I	GCNNGC	2 cut(s)	133, 2038
BstDEI	CTNAG	5 cut(s)	311, 897, 1123, 1398, 1746
BstDSI	CCRYGG	2 cut(s)	889, 1979
BstEII	GGTNACC	1 cut(s)	942
BstF5I	GGATG	5 cut(s)	59, 207, 523, 1200, 1989
BstFNI	CGCG	4 cut(s)	146, 191, 965, 2022
BstH2I	RGCGCY	1 cut(s)	1429
BstHHI	GCGC	3 cut(s)	148, 1122, 1428
BstKTI	GATC	9 cut(s)	277, 320, 382, 903, 1083, 1239, 1511, 1802, 1966
BstMAI	GTCTC	3 cut(s)	173, 482, 2018
BstMBI	GATC	9 cut(s)	274, 317, 379, 900, 1080, 1236, 1508, 1799, 1963
BstMWI	GCNNNNNNNGC	4 cut(s)	503, 971, 1415, 1913
BstNI	CCWGG	1 cut(s)	173
BstNSI	RCATGY	3 cut(s)	721, 1358, 2040
BstPI	GGTNACC	1 cut(s)	942
BstSCI	CCNGG	1 cut(s)	171
BstSFI	CTRYAG	4 cut(s)	215, 1152, 1842, 1911
BstSLI	GKGCMC	2 cut(s)	508, 556
BstUI	CGCG	4 cut(s)	146, 191, 965, 2022
BstV1I	GCAGC	5 cut(s)	610, 659, 1303, 1427, 1925
BstV2I	GAAGAC	1 cut(s)	96
BstX2I	RGATCY	5 cut(s)	900, 1080, 1236, 1508, 1799
BstXI	CCANNNNNNTGG	1 cut(s)	757
BstYI	RGATCY	5 cut(s)	900, 1080, 1236, 1508, 1799
BstZ17I	GTATAC	1 cut(s)	1607
Bsu36I	CCTNAGG	1 cut(s)	1746
BsuI	GTATCC	1 cut(s)	610
BsuRI	GGCC	4 cut(s)	28, 913, 1440, 1678
BtgI	CCRYGG	2 cut(s)	889, 1979
BtsCI	GGATG	5 cut(s)	59, 207, 523, 1200, 1989
BtsIMutI	CAGTG	6 cut(s)	135, 319, 436, 1102, 1485, 1929
BveI	ACCTGC	3 cut(s)	1145, 1159, 1321
Cac8I	GCNNGC	2 cut(s)	133, 2038
CaiI	CAGNNNCTG	2 cut(s)	799, 1067
CfoI	GCGC	3 cut(s)	148, 1122, 1428
Cfr13I	GGNCC	4 cut(s)	680, 1248, 1438, 1789
CseI	GACGC	2 cut(s)	197, 2000
Csp6I	GTAC	3 cut(s)	1019, 1046, 1148
CspCI	CAANNNNNGTGG	2 cut(s)	295, 330
CviQI	GTAC	3 cut(s)	1019, 1046, 1148
DdeI	CTNAG	5 cut(s)	311, 897, 1123, 1398, 1746
DinI	GGCGCC	1 cut(s)	1427
DpnI	GATC	9 cut(s)	276, 319, 381, 902, 1082, 1238, 1510, 1801, 1965
DpnII	GATC	9 cut(s)	274, 317, 379, 900, 1080, 1236, 1508, 1799, 1963
DrdI	GACNNNNNNGTC	2 cut(s)	119, 1487
DriI	GACNNNNNGTC	1 cut(s)	1853
DseDI	GACNNNNNNGTC	2 cut(s)	119, 1487
EaeI	YGGCCR	2 cut(s)	26, 911
Eam1104I	CTCTTC	4 cut(s)	264, 1371, 1461, 1518
Eam1105I	GACNNNNNGTC	1 cut(s)	1853
EarI	CTCTTC	4 cut(s)	264, 1371, 1461, 1518
Eco130I	CCWWGG	2 cut(s)	527, 676
Eco32I	GATATC	1 cut(s)	1380
Eco47I	GGWCC	3 cut(s)	680, 1248, 1789
Eco57I	CTGAAG	2 cut(s)	776, 1760
Eco81I	CCTNAGG	1 cut(s)	1746
Eco91I	GGTNACC	1 cut(s)	942
EcoO109I	RGGNCCY	3 cut(s)	680, 1248, 1789
EcoO65I	GGTNACC	1 cut(s)	942
EcoRI	GAATTC	2 cut(s)	177, 532
EcoRII	CCWGG	1 cut(s)	171
EcoRV	GATATC	1 cut(s)	1380
EcoT14I	CCWWGG	2 cut(s)	527, 676
EcoT22I	ATGCAT	1 cut(s)	823
EgeI	GGCGCC	1 cut(s)	1427
EheI	GGCGCC	1 cut(s)	1427
ErhI	CCWWGG	2 cut(s)	527, 676
Esp3I	CGTCTC	1 cut(s)	2018
FaqI	GGGAC	2 cut(s)	666, 1234
FauI	CCCGC	1 cut(s)	502
FauNDI	CATATG	1 cut(s)	1076
FblI	GTMKAC	3 cut(s)	1226, 1361, 1606
Fnu4HI	GCNGC	7 cut(s)	599, 673, 975, 1317, 1416, 1914, 2020
FokI	GGATG	5 cut(s)	46, 214, 510, 1207, 1996
Fsp4HI	GCNGC	7 cut(s)	599, 673, 975, 1317, 1416, 1914, 2020
FspBI	CTAG	3 cut(s)	677, 1343, 1797
GlaI	GCGC	3 cut(s)	147, 1121, 1427
GluI	GCNGC	7 cut(s)	599, 673, 975, 1317, 1416, 1914, 2020
GsuI	CTGGAG	1 cut(s)	155
HaeII	RGCGCY	1 cut(s)	1429
HaeIII	GGCC	4 cut(s)	28, 913, 1440, 1678
HapII	CCGG	1 cut(s)	1782
HgaI	GACGC	2 cut(s)	197, 2000
HhaI	GCGC	3 cut(s)	148, 1122, 1428
Hin1I	GRCGYC	4 cut(s)	114, 689, 1426, 2011
Hin6I	GCGC	3 cut(s)	146, 1120, 1426
HinP1I	GCGC	3 cut(s)	146, 1120, 1426
HindIII	AAGCTT	1 cut(s)	1874
HpaII	CCGG	1 cut(s)	1782
HphI	GGTGA	5 cut(s)	581, 936, 1315, 1585, 1752
Hpy166II	GTNNAC	7 cut(s)	472, 554, 715, 1227, 1362, 1521, 1607
Hpy188III	TCNNGA	7 cut(s)	110, 386, 926, 1084, 1216, 1287, 1837
Hpy8I	GTNNAC	7 cut(s)	472, 554, 715, 1227, 1362, 1521, 1607
Hpy99I	CGWCG	4 cut(s)	119, 122, 125, 191
HpyAV	CCTTC	1 cut(s)	192
HpyCH4III	ACNGT	6 cut(s)	356, 622, 712, 1050, 1489, 1843
HpyCH4IV	ACGT	4 cut(s)	114, 689, 1349, 2048
HpyF10VI	GCNNNNNNNGC	4 cut(s)	503, 971, 1415, 1913
HpyF3I	CTNAG	5 cut(s)	311, 897, 1123, 1398, 1746
HpySE526I	ACGT	4 cut(s)	114, 689, 1349, 2048
Hsp92I	GRCGYC	4 cut(s)	114, 689, 1426, 2011
HspAI	GCGC	3 cut(s)	146, 1120, 1426
KasI	GGCGCC	1 cut(s)	1425
KflI	GGGWCCC	1 cut(s)	1248
KpnI	GGTACC	1 cut(s)	1151
Kzo9I	GATC	9 cut(s)	274, 317, 379, 900, 1080, 1236, 1508, 1799, 1963
LmnI	GCTCC	1 cut(s)	647
Lsp1109I	GCAGC	5 cut(s)	610, 659, 1303, 1427, 1925
LweI	GCATC	4 cut(s)	192, 436, 830, 1974
MaeI	CTAG	3 cut(s)	677, 1343, 1797
MaeII	ACGT	4 cut(s)	114, 689, 1349, 2048
MaeIII	GTNAC	6 cut(s)	210, 942, 1097, 1303, 1618, 1829
MalI	GATC	9 cut(s)	276, 319, 381, 902, 1082, 1238, 1510, 1801, 1965
MboI	GATC	9 cut(s)	274, 317, 379, 900, 1080, 1236, 1508, 1799, 1963
MflI	RGATCY	5 cut(s)	900, 1080, 1236, 1508, 1799
MhlI	GDGCHC	2 cut(s)	508, 556
MlsI	TGGCCA	2 cut(s)	28, 913
MluNI	TGGCCA	2 cut(s)	28, 913
Mly113I	GGCGCC	1 cut(s)	1426
MlyI	GAGTC	3 cut(s)	484, 821, 1932
MmeI	TCCRAC	3 cut(s)	376, 1486, 1715
Mox20I	TGGCCA	2 cut(s)	28, 913
Mph1103I	ATGCAT	1 cut(s)	823
MroXI	GAANNNNTTC	1 cut(s)	1738
MscI	TGGCCA	2 cut(s)	28, 913
MseI	TTAA	4 cut(s)	255, 636, 1161, 1166
MslI	CAYNNNNRTG	3 cut(s)	441, 722, 755
Msp20I	TGGCCA	2 cut(s)	28, 913
MspA1I	CMGCKG	1 cut(s)	653
MspI	CCGG	1 cut(s)	1782
MspR9I	CCNGG	1 cut(s)	173
Mva1269I	GAATGC	1 cut(s)	988
MvaI	CCWGG	1 cut(s)	173
MvnI	CGCG	4 cut(s)	146, 191, 965, 2022
MwoI	GCNNNNNNNGC	4 cut(s)	503, 971, 1415, 1913
NarI	GGCGCC	1 cut(s)	1426
NdeI	CATATG	1 cut(s)	1076
NdeII	GATC	9 cut(s)	274, 317, 379, 900, 1080, 1236, 1508, 1799, 1963
NlaIV	GGNNCC	8 cut(s)	302, 505, 682, 1149, 1249, 1250, 1427, 1439
NmeAIII	GCCGAG	1 cut(s)	1704
NmuCI	GTSAC	4 cut(s)	942, 1097, 1303, 1618
NsiI	ATGCAT	1 cut(s)	823
NspI	RCATGY	3 cut(s)	721, 1358, 2040
PaeI	GCATGC	1 cut(s)	2040
PciI	ACATGT	1 cut(s)	717
PctI	GAATGC	1 cut(s)	988
PdmI	GAANNNNTTC	1 cut(s)	1738
PfeI	GAWTC	8 cut(s)	415, 885, 1064, 1290, 1450, 1739, 1942, 2024
PflFI	GACNNNGTC	1 cut(s)	116
PkrI	GCNGC	7 cut(s)	600, 674, 976, 1318, 1417, 1915, 2021
PleI	GAGTC	3 cut(s)	484, 821, 1931
PluTI	GGCGCC	1 cut(s)	1429
PpsI	GAGTC	3 cut(s)	484, 821, 1931
PpuMI	RGGWCCY	3 cut(s)	680, 1248, 1789
PscI	ACATGT	1 cut(s)	717
PshBI	ATTAAT	1 cut(s)	636
Psp1406I	AACGTT	1 cut(s)	1349
Psp5II	RGGWCCY	3 cut(s)	680, 1248, 1789
Psp6I	CCWGG	1 cut(s)	171
PspEI	GGTNACC	1 cut(s)	942
PspGI	CCWGG	1 cut(s)	171
PspN4I	GGNNCC	8 cut(s)	302, 505, 682, 1149, 1249, 1250, 1427, 1439
PspPI	GGNCC	4 cut(s)	680, 1248, 1438, 1789
PspPPI	RGGWCCY	3 cut(s)	680, 1248, 1789
PstI	CTGCAG	2 cut(s)	1156, 1915
PstNI	CAGNNNCTG	2 cut(s)	799, 1067
PsuI	RGATCY	5 cut(s)	900, 1080, 1236, 1508, 1799
PsyI	GACNNNGTC	1 cut(s)	116
RsaI	GTAC	3 cut(s)	1020, 1047, 1149
RsaNI	GTAC	3 cut(s)	1019, 1046, 1148
RseI	CAYNNNNRTG	3 cut(s)	441, 722, 755
SaqAI	TTAA	4 cut(s)	255, 636, 1161, 1166
SatI	GCNGC	7 cut(s)	599, 673, 975, 1317, 1416, 1914, 2020
Sau3AI	GATC	9 cut(s)	274, 317, 379, 900, 1080, 1236, 1508, 1799, 1963
Sau96I	GGNCC	4 cut(s)	680, 1248, 1438, 1789
SbfI	CCTGCAGG	1 cut(s)	1156
SchI	GAGTC	3 cut(s)	484, 821, 1932
ScrFI	CCNGG	1 cut(s)	173
SdaI	CCTGCAGG	1 cut(s)	1156
SduI	GDGCHC	2 cut(s)	508, 556
SfaNI	GCATC	4 cut(s)	192, 436, 830, 1974
SfcI	CTRYAG	4 cut(s)	215, 1152, 1842, 1911
SfoI	GGCGCC	1 cut(s)	1427
SinI	GGWCC	3 cut(s)	680, 1248, 1789
SmiMI	CAYNNNNRTG	3 cut(s)	441, 722, 755
SmlI	CTYRAG	1 cut(s)	384
SmoI	CTYRAG	1 cut(s)	384
SphI	GCATGC	1 cut(s)	2040
Sse8387I	CCTGCAGG	1 cut(s)	1156
SsiI	CCGC	7 cut(s)	495, 645, 651, 771, 965, 975, 2020
SspDI	GGCGCC	1 cut(s)	1425
SspI	AATATT	1 cut(s)	1974
SspMI	CTAG	3 cut(s)	677, 1343, 1797
StyD4I	CCNGG	1 cut(s)	171
StyI	CCWWGG	2 cut(s)	527, 676
TaaI	ACNGT	6 cut(s)	356, 622, 712, 1050, 1489, 1843
TaiI	ACGT	4 cut(s)	117, 692, 1352, 2051
TaqI	TCGA	1 cut(s)	1329
TatI	WGTACW	1 cut(s)	1018
TauI	GCSGC	2 cut(s)	977, 2022
TfiI	GAWTC	8 cut(s)	415, 885, 1064, 1290, 1450, 1739, 1942, 2024
Tru1I	TTAA	4 cut(s)	255, 636, 1161, 1166
Tru9I	TTAA	4 cut(s)	255, 636, 1161, 1166
TscAI	CASTG	6 cut(s)	142, 319, 443, 1102, 1492, 1936
TseFI	GTSAC	4 cut(s)	942, 1097, 1303, 1618
TseI	GCWGC	5 cut(s)	598, 672, 1316, 1415, 1913
Tsp45I	GTSAC	4 cut(s)	942, 1097, 1303, 1618
TspDTI	ATGAA	9 cut(s)	525, 597, 753, 1125, 1188, 1255, 1358, 1595, 1806
TspGWI	ACGGA	5 cut(s)	90, 878, 982, 1954, 1968
TspRI	CASTG	6 cut(s)	142, 319, 443, 1102, 1492, 1936
Tth111I	GACNNNGTC	1 cut(s)	116
VneI	GTGCAC	1 cut(s)	552
VpaK11BI	GGWCC	3 cut(s)	680, 1248, 1789
VspI	ATTAAT	1 cut(s)	636
XapI	RAATTY	5 cut(s)	177, 340, 532, 1088, 1656
XceI	RCATGY	3 cut(s)	721, 1358, 2040
XcmI	CCANNNNNNNNNTGG	3 cut(s)	311, 1698, 1977
XmaJI	CCTAGG	1 cut(s)	676
XmiI	GTMKAC	3 cut(s)	1226, 1361, 1606
XmnI	GAANNNNTTC	1 cut(s)	1738
XspI	CTAG	3 cut(s)	677, 1343, 1797
ZraI	GACGTC	2 cut(s)	115, 690
Zsp2I	ATGCAT	1 cut(s)	823

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.