Basic Information

Type: gene

Biological Identity

Species

prunus_persica

Chromosome

Pp04

Orthogroup ID

OG0005134

Physical Location & Seq

Genomic Coordinates Forward (+)

22882754 .. 22887781

Gene Structure 5028 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Prupe.4G274500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2058 bp

ATGGGAAGGAGGTGTAGGGTTTGGTGGCCAAAGCAACTCTCATTGAGCACACCATCATCCTGCTCCAATTTCTTGCTGGGTTGGTTCATTTCGTCGTCTTCGTCTTCTCTTGACGTTGTCGTAGCTTTTGCCTGCACTGAACAAGCGCTTTCTGATAAAAAATTATGCATTCAGGGAATCCTTCATGACACAAATGGAAGGATGCCTGTGTTGCTCCAGGATAAGTCAATGTTGTGTATAGTGGGTCAATTTTTTAAAGTTCACTCAAAAGAAGATCAATATCATTCTTCTTGTTGTGGGTGCCACACTCTCAATGGATCACTAGAACAATGTAGGCAAACTTTTGTAGAAAGCAATTATTGGATCCAGATGCTATGTGATCCTCAAGAACAAGTTGGAACAGAAATTAGTTGGATTCCTAAACTGCATCACATCCACTGGAATGGGCAATTGGTGTTTCCATGTGATATCCACTTAATATTCTACGAGACCCCAGCCTATGGTGCTCACCATTTCTCATTACATCCTTGGAATTCATTTGATCAAGTGAATGCTCCTGAGAGAAAACCCAAGTGGGTTGATGAACTTCACCAGAAGCAGCCACTCCTTGACTTGGATACGGTCATTCTGGCAATCAATAGCTCCGCTGCTGCTGACAAAGTTTTCGAAAGATGCATGGGTCCCAAGAAATCTACTGTGCGCTTTTCCACAGTTTACATGTTTCTTGCCTTCACATGGCAACTATTTGCTGTGTCTGTGGCTTCATTGTCCATGTTATTCTATGTCATTGTTCAGTTTCTCTATAGGCTTCTGAAGTATGCATCAGATTCATGGGTATACATCATATCAGTAAAGGTATTCAGCAGTTCAAGGATAAATATCAGAATCCGTTGTTCTCAGATCTTGTATTGGCCAATCTTTCTTCAAGATAATGGCACAAGGTCACTATCAAGTGTTGAATATGCGGAGAAAGCCGCATTGCATAAGCATTCCATGTGGTCAAGTTTAGCTGTTGATGTACTTCTGGGAAACTTGTTCGGTTTGGCACTGCTGTATCATGCAGAATCTGCTTGCATGTGGGTCCTGAAATTTGCCAGTGACATTACAAATGAGTTATTGCGCTCAGGTTGTGTGTGGTTAATGGGAGTCCCCGCAGGTTTTAAGTTAAACACGGAATTGGCAGGAGTTCTTGGGATGATTTCACTAACTGCTATCCAGATTTGGTCTACCATTTGGATCTTTTTGGGGTTTCACTTCATTTATTTCATTAGAGGACTTGCTATATCAGGAATCATTTTTGGGGTGACTGTACCTGCTGCTTTGATAAAAGACTTGATTGCACTAGCAACATTACATGTGTCCACTCTTCACTGGTTGATATCACTTTTATACTCGACTCAGATACAGGCATTGGCAGCTTTGTGGCGCCTTTTCAGGGGTCGAAAGTGGAATCCTCTTCGTCAGAGATTAGATAGCTATGACTATACTGTCAAGCAACACATCGTTGGATCTCTTCTGTTCACACCACTCCTGCTTCTATTACCGACAACTTCTGTTTTCTATATTTTCTTTACCATTATGAATACAACCATCAGCCTTATCTATATACTGATTGAAATCACTATATCTGTTATTCATGCCACACCTTACATCAAAATTTTCCTTTGGTTGGTCATGCCAAAAAGATTTCCCTCTGGGATATGGTTTGAAATTGTATCTGTTTGGAGTGATTGCATTTATTCACATAAGGATATTAGTTCACCAGCAGATAAATTGCAGAGTGAAAAGGGCCTAACTGGAGAGAAAGCTTCTGTTGTGGTTTCATTTCTTCATAGCAACTTCTTGACTGTAGGACAAATAGTCATGCCTCACTACAACAAGATTCTTTCTGGGAAACCTCGGACATTGGTTGCTACAGCAGCTTATGGAGTCCTTACTGGCAGACGGATTCCATCTACAATTGGGACTGATCTTCCAATATTTCCATGGATGCTAATCTCCTACAAAGAGTATTGGCGGCTTTGCCACGATTCAATTCTTGCATGCTACAGAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000506 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006497 GO:0006505 GO:0006506 GO:0006629 GO:0006643 GO:0006644 GO:0006650 GO:0006661 GO:0006664 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009059 GO:0009247 GO:0009987 GO:0012505 GO:0016020 GO:0019538 GO:0019637 GO:0031984 GO:0032991 GO:0034645 GO:0036211 GO:0042157 GO:0042158 GO:0042175 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046467 GO:0046474 GO:0046486 GO:0046488 GO:0071704 GO:0090407 GO:0098796 GO:0098827 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1902494 GO:1903509 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00563 ko01100 map00563 map01100

Pfam Domains

Protein Families

Gpi1

Protein Analysis

686

Amino Acids

78.03

Weight (kDa)

8.95

Isoelectric Point (pI)

41.06

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005134)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57170 AT3G57170
fragaria_vesca	FvH4_6g24900 FvH4_6g24900 FvH4_6g24900 FvH4_6g24900 FvH4_6g24900 FvH4_6g24900 FvH4_6g24900 FvH4_6g24900
malus_domestica	MD07G1151600.v1.1 MD12G1089200.v1.1 MD14G1081800.v1.1
prunus_persica	Prupe.4G274500_v2.0.a1 Prupe.5G000500_v2.0.a1
pyrus_communis	pycom07g15000 pycom12g07440 pycom14g07110
rosa_chinensis	RchiOBHm_Chr2g0124601
rosa_laevigata	RLG00000018807
rosa_multiflora	Rmu_sc0042054.1_g000001
rosa_roxburghii	Rroxscaffold_2G00119850
rosa_rugosa	Rorug02G0251500 Rorug02G0251500
rosa_samantha	Rh2AG310200 Rh2BG318700 Rh2CG297100 Rh2CG302000 Rh2DG333900
rosa_wichuraiana	Rw2G025090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1487
Acc36I	ACCTGC	2 cut(s)	1145, 1321
AccB1I	GGYRCC	2 cut(s)	300, 1425
AccB7I	CCANNNNNTGG	1 cut(s)	500
AccI	GTMKAC	2 cut(s)	837, 1226
AciI	CCGC	5 cut(s)	645, 965, 975, 1152, 2017
AclWI	GGATC	6 cut(s)	325, 358, 371, 374, 1244, 1516
AcoI	YGGCCR	2 cut(s)	26, 911
AcsI	RAATTY	3 cut(s)	532, 1088, 1656
AcuI	CTGAAG	1 cut(s)	833
AcyI	GRCGYC	1 cut(s)	1426
AfaI	GTAC	2 cut(s)	1020, 1311
AfeI	AGCGCT	1 cut(s)	147
AfiI	CCNNNNNNNGG	5 cut(s)	500, 613, 735, 1435, 1669
AflIII	ACRYGT	2 cut(s)	717, 1354
AgsI	TTSAA	6 cut(s)	870, 926, 959, 1616, 1709, 2034
AhdI	GACNNNNNGTC	1 cut(s)	1859
AjnI	CCWGG	1 cut(s)	216
AjuI	GAANNNNNNNTTGG	2 cut(s)	434, 466
AluBI	AGCT	7 cut(s)	125, 642, 1010, 1418, 1476, 1808, 1922
AluI	AGCT	7 cut(s)	125, 642, 1010, 1418, 1476, 1808, 1922
Alw21I	GWGCWC	2 cut(s)	50, 508
Alw26I	GTCTC	1 cut(s)	482
AlwI	GGATC	6 cut(s)	325, 358, 371, 374, 1244, 1516
AlwNI	CAGNNNCTG	1 cut(s)	1067
Aor51HI	AGCGCT	1 cut(s)	147
AoxI	GGCC	3 cut(s)	26, 911, 1789
ApeKI	GCWGC	6 cut(s)	598, 647, 650, 1316, 1415, 1919
ApoI	RAATTY	3 cut(s)	532, 1088, 1656
ArsI	GACNNNNNNTTYG	2 cut(s)	647, 679
AspLEI	GCGC	4 cut(s)	148, 702, 1122, 1428
AspS9I	GGNCC	3 cut(s)	680, 1081, 1789
AsuHPI	GGTGA	4 cut(s)	500, 581, 1315, 1752
AsuII	TTCGAA	1 cut(s)	666
AvaII	GGWCC	2 cut(s)	680, 1081
BaeI	ACNNNNGTAYC	2 cut(s)	1037, 1070
BalI	TGGCCA	2 cut(s)	28, 913
BamHI	GGATCC	1 cut(s)	363
BanI	GGYRCC	2 cut(s)	300, 1425
BbsI	GAAGAC	2 cut(s)	90, 96
Bbv12I	GWGCWC	2 cut(s)	50, 508
BbvI	GCAGC	6 cut(s)	610, 634, 637, 1303, 1427, 1931
BccI	CCATC	3 cut(s)	61, 1598, 1960
BciT130I	CCWGG	1 cut(s)	218
BciVI	GTATCC	1 cut(s)	610
BclI	TGATCA	1 cut(s)	541
BcoDI	GTCTC	1 cut(s)	482
BfaI	CTAG	2 cut(s)	323, 1343
BfmI	CTRYAG	4 cut(s)	802, 1848, 1914, 2047
BfoI	RGCGCY	2 cut(s)	149, 1429
BfuAI	ACCTGC	2 cut(s)	1145, 1321
BfuI	GTATCC	1 cut(s)	610
BglII	AGATCT	1 cut(s)	900
BisI	GCNGC	8 cut(s)	599, 648, 651, 975, 1317, 1416, 1920, 2018
BlsI	GCNGC	8 cut(s)	600, 649, 652, 976, 1318, 1417, 1921, 2019
Bme1390I	CCNGG	1 cut(s)	218
Bme18I	GGWCC	2 cut(s)	680, 1081
BmeRI	GACNNNNNGTC	1 cut(s)	1859
BmgT120I	GGNCC	3 cut(s)	680, 1081, 1789
BmiI	GGNNCC	6 cut(s)	302, 365, 681, 682, 1082, 1427
BmrFI	CCNGG	1 cut(s)	218
BmsI	GCATC	6 cut(s)	192, 360, 436, 662, 830, 1980
BpiI	GAAGAC	2 cut(s)	90, 96
BpmI	CTGGAG	2 cut(s)	200, 1818
Bpu10I	CCTNAGC	1 cut(s)	1123
Bpu14I	TTCGAA	1 cut(s)	666
BpuEI	CTTGAG	1 cut(s)	369
BsaBI	GATNNNNATC	4 cut(s)	279, 878, 1616, 1994
BsaHI	GRCGYC	1 cut(s)	1426
BsaI	GGTCTC	1 cut(s)	482
BsaJI	CCNNGG	3 cut(s)	527, 1898, 1985
Bsc4I	CCNNNNNNNGG	5 cut(s)	500, 613, 735, 1435, 1669
Bse1I	ACTGG	5 cut(s)	443, 1095, 1376, 1801, 1942
Bse3DI	GCAATG	1 cut(s)	977
Bse8I	GATNNNNATC	4 cut(s)	279, 878, 1616, 1994
BseBI	CCWGG	1 cut(s)	218
BseDI	CCNNGG	3 cut(s)	527, 1898, 1985
BseGI	GGATG	6 cut(s)	56, 207, 432, 523, 1200, 1995
BseJI	GATNNNNATC	4 cut(s)	279, 878, 1616, 1994
BseLI	CCNNNNNNNGG	5 cut(s)	500, 613, 735, 1435, 1669
BseMI	GCAATG	1 cut(s)	977
BseMII	CTCAG	4 cut(s)	549, 911, 1137, 1412
BseNI	ACTGG	5 cut(s)	443, 1095, 1376, 1801, 1942
BseXI	GCAGC	6 cut(s)	610, 634, 637, 1303, 1427, 1931
BseYI	CCCAGC	2 cut(s)	76, 493
BsgI	GTGCAG	1 cut(s)	118
BshFI	GGCC	3 cut(s)	28, 913, 1791
BshNI	GGYRCC	2 cut(s)	300, 1425
BsiHKAI	GWGCWC	2 cut(s)	50, 508
BslFI	GGGAC	3 cut(s)	666, 1133, 1978
BslI	CCNNNNNNNGG	5 cut(s)	500, 613, 735, 1435, 1669
BsmAI	GTCTC	1 cut(s)	482
BsmFI	GGGAC	3 cut(s)	666, 1133, 1978
BsmI	GAATGC	3 cut(s)	168, 556, 988
BsnI	GGCC	3 cut(s)	28, 913, 1791
Bso31I	GGTCTC	1 cut(s)	482
Bsp119I	TTCGAA	1 cut(s)	666
Bsp1286I	GDGCHC	2 cut(s)	50, 508
Bsp143I	GATC	9 cut(s)	274, 317, 363, 379, 541, 900, 1236, 1508, 1969
Bsp19I	CCATGG	1 cut(s)	1985
BspACI	CCGC	5 cut(s)	645, 965, 975, 1152, 2017
BspANI	GGCC	3 cut(s)	28, 913, 1791
BspCNI	CTCAG	4 cut(s)	550, 910, 1136, 1411
BspHI	TCATGA	1 cut(s)	184
BspLI	GGNNCC	6 cut(s)	302, 365, 681, 682, 1082, 1427
BspMI	ACCTGC	2 cut(s)	1145, 1321
BspPI	GGATC	6 cut(s)	325, 358, 371, 374, 1244, 1516
BspT104I	TTCGAA	1 cut(s)	666
BspT107I	GGYRCC	2 cut(s)	300, 1425
BspTNI	GGTCTC	1 cut(s)	482
BsrDI	GCAATG	1 cut(s)	977
BsrI	ACTGG	5 cut(s)	443, 1095, 1376, 1801, 1942
BssECI	CCNNGG	3 cut(s)	527, 1898, 1985
BssMI	GATC	9 cut(s)	274, 317, 363, 379, 541, 900, 1236, 1508, 1969
BssNAI	GTATAC	1 cut(s)	838
BssNI	GRCGYC	1 cut(s)	1426
BssT1I	CCWWGG	2 cut(s)	527, 1985
Bst1107I	GTATAC	1 cut(s)	838
Bst2UI	CCWGG	1 cut(s)	218
Bst4CI	ACNGT	6 cut(s)	622, 697, 712, 1309, 1489, 1849
Bst6I	CTCTTC	3 cut(s)	1371, 1461, 1518
BstACI	GRCGYC	1 cut(s)	1426
BstAPI	GCANNNNNTGC	1 cut(s)	1067
BstBI	TTCGAA	1 cut(s)	666
BstC8I	GCNNGC	3 cut(s)	133, 1072, 2044
BstDEI	CTNAG	4 cut(s)	558, 897, 1123, 1398
BstDSI	CCRYGG	1 cut(s)	1985
BstF5I	GGATG	6 cut(s)	56, 207, 432, 523, 1200, 1995
BstH2I	RGCGCY	2 cut(s)	149, 1429
BstHHI	GCGC	4 cut(s)	148, 702, 1122, 1428
BstKTI	GATC	9 cut(s)	277, 320, 366, 382, 544, 903, 1239, 1511, 1972
BstMAI	GTCTC	1 cut(s)	482
BstMBI	GATC	9 cut(s)	274, 317, 363, 379, 541, 900, 1236, 1508, 1969
BstMWI	GCNNNNNNNGC	6 cut(s)	211, 503, 971, 1067, 1415, 1919
BstNI	CCWGG	1 cut(s)	218
BstNSI	RCATGY	4 cut(s)	721, 1078, 1358, 2046
BstSCI	CCNGG	1 cut(s)	216
BstSFI	CTRYAG	4 cut(s)	802, 1848, 1914, 2047
BstV1I	GCAGC	6 cut(s)	610, 634, 637, 1303, 1427, 1931
BstV2I	GAAGAC	2 cut(s)	90, 96
BstX2I	RGATCY	4 cut(s)	363, 900, 1236, 1508
BstXI	CCANNNNNNTGG	1 cut(s)	443
BstYI	RGATCY	4 cut(s)	363, 900, 1236, 1508
BstZ17I	GTATAC	1 cut(s)	838
BsuI	GTATCC	1 cut(s)	610
BsuRI	GGCC	3 cut(s)	28, 913, 1791
BtgI	CCRYGG	1 cut(s)	1985
BtsCI	GGATG	6 cut(s)	56, 207, 432, 523, 1200, 1995
BtsI	GCAGTG	1 cut(s)	1046
BtsIMutI	CAGTG	5 cut(s)	135, 436, 1046, 1102, 1369
BveI	ACCTGC	2 cut(s)	1145, 1321
Cac8I	GCNNGC	3 cut(s)	133, 1072, 2044
CaiI	CAGNNNCTG	1 cut(s)	1067
CciI	TCATGA	1 cut(s)	184
CfoI	GCGC	4 cut(s)	148, 702, 1122, 1428
Cfr13I	GGNCC	3 cut(s)	680, 1081, 1789
Csp6I	GTAC	2 cut(s)	1019, 1310
CviQI	GTAC	2 cut(s)	1019, 1310
DdeI	CTNAG	4 cut(s)	558, 897, 1123, 1398
DinI	GGCGCC	1 cut(s)	1427
DpnI	GATC	9 cut(s)	276, 319, 365, 381, 543, 902, 1238, 1510, 1971
DpnII	GATC	9 cut(s)	274, 317, 363, 379, 541, 900, 1236, 1508, 1969
DraI	TTTAAA	1 cut(s)	256
DrdI	GACNNNNNNGTC	1 cut(s)	1487
DriI	GACNNNNNGTC	1 cut(s)	1859
DseDI	GACNNNNNNGTC	1 cut(s)	1487
EaeI	YGGCCR	2 cut(s)	26, 911
Eam1104I	CTCTTC	3 cut(s)	1371, 1461, 1518
Eam1105I	GACNNNNNGTC	1 cut(s)	1859
EarI	CTCTTC	3 cut(s)	1371, 1461, 1518
Eco130I	CCWWGG	2 cut(s)	527, 1985
Eco31I	GGTCTC	1 cut(s)	482
Eco32I	GATATC	2 cut(s)	469, 1380
Eco47I	GGWCC	2 cut(s)	680, 1081
Eco47III	AGCGCT	1 cut(s)	147
Eco57I	CTGAAG	1 cut(s)	833
EcoO109I	RGGNCCY	3 cut(s)	680, 1081, 1789
EcoRI	GAATTC	1 cut(s)	532
EcoRII	CCWGG	1 cut(s)	216
EcoRV	GATATC	2 cut(s)	469, 1380
EcoT14I	CCWWGG	2 cut(s)	527, 1985
EcoT22I	ATGCAT	3 cut(s)	170, 677, 823
EgeI	GGCGCC	1 cut(s)	1427
EheI	GGCGCC	1 cut(s)	1427
ErhI	CCWWGG	2 cut(s)	527, 1985
FaqI	GGGAC	3 cut(s)	666, 1133, 1978
FauI	CCCGC	1 cut(s)	1159
FbaI	TGATCA	1 cut(s)	541
FblI	GTMKAC	2 cut(s)	837, 1226
Fnu4HI	GCNGC	8 cut(s)	599, 648, 651, 975, 1317, 1416, 1920, 2018
FokI	GGATG	6 cut(s)	43, 214, 419, 510, 1207, 2002
Fsp4HI	GCNGC	8 cut(s)	599, 648, 651, 975, 1317, 1416, 1920, 2018
FspBI	CTAG	2 cut(s)	323, 1343
GlaI	GCGC	4 cut(s)	147, 701, 1121, 1427
GluI	GCNGC	8 cut(s)	599, 648, 651, 975, 1317, 1416, 1920, 2018
GsaI	CCCAGC	2 cut(s)	80, 497
GsuI	CTGGAG	2 cut(s)	200, 1818
HaeII	RGCGCY	2 cut(s)	149, 1429
HaeIII	GGCC	3 cut(s)	28, 913, 1791
HhaI	GCGC	4 cut(s)	148, 702, 1122, 1428
Hin1I	GRCGYC	1 cut(s)	1426
Hin6I	GCGC	4 cut(s)	146, 700, 1120, 1426
HinP1I	GCGC	4 cut(s)	146, 700, 1120, 1426
HindIII	AAGCTT	1 cut(s)	1806
HphI	GGTGA	4 cut(s)	500, 581, 1315, 1752
Hpy166II	GTNNAC	7 cut(s)	262, 715, 838, 1227, 1362, 1521, 1760
Hpy188I	TCNGA	8 cut(s)	154, 813, 826, 884, 900, 1401, 1464, 1902
Hpy8I	GTNNAC	7 cut(s)	262, 715, 838, 1227, 1362, 1521, 1760
Hpy99I	CGWCG	1 cut(s)	97
HpyAV	CCTTC	3 cut(s)	191, 192, 739
HpyCH4III	ACNGT	6 cut(s)	622, 697, 712, 1309, 1489, 1849
HpyCH4IV	ACGT	1 cut(s)	114
HpyF10VI	GCNNNNNNNGC	6 cut(s)	211, 503, 971, 1067, 1415, 1919
HpyF3I	CTNAG	4 cut(s)	558, 897, 1123, 1398
HpySE526I	ACGT	1 cut(s)	114
Hsp92I	GRCGYC	1 cut(s)	1426
HspAI	GCGC	4 cut(s)	146, 700, 1120, 1426
KasI	GGCGCC	1 cut(s)	1425
KflI	GGGWCCC	1 cut(s)	680
Ksp22I	TGATCA	1 cut(s)	541
Kzo9I	GATC	9 cut(s)	274, 317, 363, 379, 541, 900, 1236, 1508, 1969
LmnI	GCTCC	4 cut(s)	68, 219, 559, 647
Lsp1109I	GCAGC	6 cut(s)	610, 634, 637, 1303, 1427, 1931
LweI	GCATC	6 cut(s)	192, 360, 436, 662, 830, 1980
MaeI	CTAG	2 cut(s)	323, 1343
MaeII	ACGT	1 cut(s)	114
MaeIII	GTNAC	3 cut(s)	942, 1097, 1303
MalI	GATC	9 cut(s)	276, 319, 365, 381, 543, 902, 1238, 1510, 1971
MboI	GATC	9 cut(s)	274, 317, 363, 379, 541, 900, 1236, 1508, 1969
MfeI	CAATTG	2 cut(s)	449, 1959
MflI	RGATCY	4 cut(s)	363, 900, 1236, 1508
MhlI	GDGCHC	2 cut(s)	50, 508
MlsI	TGGCCA	2 cut(s)	28, 913
MluNI	TGGCCA	2 cut(s)	28, 913
Mly113I	GGCGCC	1 cut(s)	1426
MlyI	GAGTC	3 cut(s)	1155, 1390, 1938
MmeI	TCCRAC	3 cut(s)	376, 392, 1486
MnlI	CCTC	7 cut(s)	3, 393, 1265, 1464, 1702, 1878, 1908
Mox20I	TGGCCA	2 cut(s)	28, 913
Mph1103I	ATGCAT	3 cut(s)	170, 677, 823
MscI	TGGCCA	2 cut(s)	28, 913
MseI	TTAA	5 cut(s)	255, 476, 1139, 1161, 1166
MslI	CAYNNNNRTG	1 cut(s)	441
Msp20I	TGGCCA	2 cut(s)	28, 913
MspA1I	CMGCKG	1 cut(s)	647
MspR9I	CCNGG	1 cut(s)	218
MunI	CAATTG	2 cut(s)	449, 1959
Mva1269I	GAATGC	3 cut(s)	168, 556, 988
MvaI	CCWGG	1 cut(s)	218
MwoI	GCNNNNNNNGC	6 cut(s)	211, 503, 971, 1067, 1415, 1919
NarI	GGCGCC	1 cut(s)	1426
NcoI	CCATGG	1 cut(s)	1985
NdeII	GATC	9 cut(s)	274, 317, 363, 379, 541, 900, 1236, 1508, 1969
NlaIV	GGNNCC	6 cut(s)	302, 365, 681, 682, 1082, 1427
NmuCI	GTSAC	3 cut(s)	942, 1097, 1303
NsiI	ATGCAT	3 cut(s)	170, 677, 823
NspI	RCATGY	4 cut(s)	721, 1078, 1358, 2046
NspV	TTCGAA	1 cut(s)	666
PaeI	GCATGC	1 cut(s)	2046
PagI	TCATGA	1 cut(s)	184
PciI	ACATGT	2 cut(s)	717, 1354
PcsI	WCGNNNNNNNCGW	1 cut(s)	98
PctI	GAATGC	3 cut(s)	168, 556, 988
PflFI	GACNNNGTC	1 cut(s)	116
PflMI	CCANNNNNTGG	1 cut(s)	500
PfoI	TCCNGGA	1 cut(s)	216
PkrI	GCNGC	8 cut(s)	600, 649, 652, 976, 1318, 1417, 1921, 2019
PleI	GAGTC	3 cut(s)	1154, 1390, 1937
PluTI	GGCGCC	1 cut(s)	1429
PpsI	GAGTC	3 cut(s)	1154, 1390, 1937
PpuMI	RGGWCCY	2 cut(s)	680, 1081
PscI	ACATGT	2 cut(s)	717, 1354
Psp5II	RGGWCCY	2 cut(s)	680, 1081
Psp6I	CCWGG	1 cut(s)	216
PspFI	CCCAGC	2 cut(s)	76, 493
PspGI	CCWGG	1 cut(s)	216
PspN4I	GGNNCC	6 cut(s)	302, 365, 681, 682, 1082, 1427
PspPI	GGNCC	3 cut(s)	680, 1081, 1789
PspPPI	RGGWCCY	2 cut(s)	680, 1081
PstNI	CAGNNNCTG	1 cut(s)	1067
PsuI	RGATCY	4 cut(s)	363, 900, 1236, 1508
PsyI	GACNNNGTC	1 cut(s)	116
RsaI	GTAC	2 cut(s)	1020, 1311
RsaNI	GTAC	2 cut(s)	1019, 1310
RseI	CAYNNNNRTG	1 cut(s)	441
SaqAI	TTAA	5 cut(s)	255, 476, 1139, 1161, 1166
SatI	GCNGC	8 cut(s)	599, 648, 651, 975, 1317, 1416, 1920, 2018
Sau3AI	GATC	9 cut(s)	274, 317, 363, 379, 541, 900, 1236, 1508, 1969
Sau96I	GGNCC	3 cut(s)	680, 1081, 1789
SchI	GAGTC	3 cut(s)	1155, 1390, 1938
ScrFI	CCNGG	1 cut(s)	218
SduI	GDGCHC	2 cut(s)	50, 508
SfaNI	GCATC	6 cut(s)	192, 360, 436, 662, 830, 1980
SfcI	CTRYAG	4 cut(s)	802, 1848, 1914, 2047
SfoI	GGCGCC	1 cut(s)	1427
SfuI	TTCGAA	1 cut(s)	666
SinI	GGWCC	2 cut(s)	680, 1081
SmiMI	CAYNNNNRTG	1 cut(s)	441
SmlI	CTYRAG	1 cut(s)	384
SmoI	CTYRAG	1 cut(s)	384
SphI	GCATGC	1 cut(s)	2046
SsiI	CCGC	5 cut(s)	645, 965, 975, 1152, 2017
SspDI	GGCGCC	1 cut(s)	1425
SspI	AATATT	2 cut(s)	480, 1980
SspMI	CTAG	2 cut(s)	323, 1343
StyD4I	CCNGG	1 cut(s)	216
StyI	CCWWGG	2 cut(s)	527, 1985
TaaI	ACNGT	6 cut(s)	622, 697, 712, 1309, 1489, 1849
TaiI	ACGT	1 cut(s)	117
TaqI	TCGA	3 cut(s)	666, 1394, 1441
TatI	WGTACW	1 cut(s)	1018
TauI	GCSGC	2 cut(s)	977, 2020
Tru1I	TTAA	5 cut(s)	255, 476, 1139, 1161, 1166
Tru9I	TTAA	5 cut(s)	255, 476, 1139, 1161, 1166
TscAI	CASTG	5 cut(s)	142, 443, 1053, 1102, 1376
TseFI	GTSAC	3 cut(s)	942, 1097, 1303
TseI	GCWGC	6 cut(s)	598, 647, 650, 1316, 1415, 1919
Tsp45I	GTSAC	3 cut(s)	942, 1097, 1303
TspGWI	ACGGA	3 cut(s)	878, 1187, 1960
TspRI	CASTG	5 cut(s)	142, 443, 1053, 1102, 1376
Tth111I	GACNNNGTC	1 cut(s)	116
Van91I	CCANNNNNTGG	1 cut(s)	500
VpaK11BI	GGWCC	2 cut(s)	680, 1081
XapI	RAATTY	3 cut(s)	532, 1088, 1656
XceI	RCATGY	4 cut(s)	721, 1078, 1358, 2046
XcmI	CCANNNNNNNNNTGG	3 cut(s)	73, 311, 1983
XmiI	GTMKAC	2 cut(s)	837, 1226
XspI	CTAG	2 cut(s)	323, 1343
Zsp2I	ATGCAT	3 cut(s)	170, 677, 823

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.