Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0012151

Physical Location & Seq

Genomic Coordinates Forward (+)

13050522 .. 13052361

Gene Structure 1840 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1095300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 723 bp

ATGCAGCCCGCTGATCATCAAGGCCAAGAACTCTACCGATTTCTTGCCCAAAACGGCTTAATCAACGTGAATAATGATCATCAGTTATCAGCTATGCAAAGCTTTTGCAGCTCTTCTTCTAATAATTATAGTTATTACCCTCTGGAAGTGTCTGGGGTAAGTACTGAGCATGACGCCTCACCTCAAGATAGAGCCCTTGCTGCTCTCAAGAATCACAAGGAGGCCGAGAAGAGAAGGAGAGAGCGAATCAACTCCCATCTCGATAAGCTTCGAGGCCTTCTTCCTTGCAATTCCAAGACAGACAAAGCCTCCCTGTTAGCCAAGGTGGTCCAGAGAGTGAAAGAGCTGAAACAACAAACGTCAGAGATCGCAGAGCTGGAGACCTTCCCTTCTGAAACTGATGAAATCACTGTACTTTCTTCAAATGATTATACAAGCGATGGAAGAATTGTTTTCAAGGCCTCTTTGTGCTGCGAGGACCGCTCCGACCTCCTACCTGACCTAATTGAAATACTGAAATCCCTCCATTTGAGGACAATCAAAGCTGAAATTGCCACTCTTGGAGGAAGAATTCGCAACGTGCTTATCGTTGCCGCAGACCAAGATCACACGATTGAATCAGTCCATTTCTTGCAGAATGCGTTGAAGTCATTACTTGAACGCTCCAACTCTAGTGAAAGATCAAAAAGAAGACGGGTATTAGACCGCACATTAGTCCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006950 GO:0006970 GO:0006972 GO:0008150 GO:0009507 GO:0009526 GO:0009536 GO:0009628 GO:0009651 GO:0009889 GO:0009941 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031967 GO:0031975 GO:0042221 GO:0042493 GO:0042538 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1901698 GO:1901700 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH

Protein Analysis

241

Amino Acids

27.1

Weight (kDa)

7.09

Isoelectric Point (pI)

53.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HLH	PF00010	71 - 117	1.6e-11	Helix-loop-helix DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012151)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41130 AT3G56770
fragaria_vesca	FvH4_3g28400
malus_domestica	MD12G1095300.v1.1 MD14G1091000.v1.1
prunus_persica	Prupe.7G031500_v2.0.a1
pyrus_communis	pycom12g08210 pycom14g08270
rosa_chinensis	RchiOBHm_Chr5g0053301
rosa_laevigata	RLG00000034769
rosa_multiflora	Rmu_co8065512.1_g000001 Rmu_sc0001966.1_g000015
rosa_roxburghii	Rroxscaffold_1G00029060
rosa_rugosa	Rorug05G0267500
rosa_samantha	Rh5AG340100 Rh5BG350800 Rh5CG376900 Rh5DG364700
rosa_wichuraiana	Rw5G032110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	483
AciI	CCGC	4 cut(s)	9, 481, 594, 706
AcsI	RAATTY	1 cut(s)	570
AcyI	GRCGYC	1 cut(s)	174
AfaI	GTAC	2 cut(s)	163, 414
AgsI	TTSAA	6 cut(s)	423, 457, 509, 617, 646, 659
AluBI	AGCT	7 cut(s)	92, 102, 111, 268, 346, 376, 545
AluI	AGCT	7 cut(s)	92, 102, 111, 268, 346, 376, 545
Alw26I	GTCTC	1 cut(s)	374
AoxI	GGCC	4 cut(s)	22, 222, 274, 459
ApeKI	GCWGC	4 cut(s)	4, 108, 200, 471
ApoI	RAATTY	1 cut(s)	570
AspS9I	GGNCC	2 cut(s)	328, 478
AsuHPI	GGTGA	1 cut(s)	171
AvaII	GGWCC	2 cut(s)	328, 478
BanII	GRGCYC	1 cut(s)	196
BbsI	GAAGAC	1 cut(s)	697
BbvI	GCAGC	4 cut(s)	16, 120, 187, 458
BccI	CCATC	2 cut(s)	264, 434
BceAI	ACGGC	1 cut(s)	70
BclI	TGATCA	2 cut(s)	13, 76
BcoDI	GTCTC	1 cut(s)	374
BfaI	CTAG	1 cut(s)	672
BisI	GCNGC	5 cut(s)	5, 109, 201, 472, 594
BlsI	GCNGC	5 cut(s)	6, 110, 202, 473, 595
BmcAI	AGTACT	1 cut(s)	163
Bme18I	GGWCC	2 cut(s)	328, 478
BmgT120I	GGNCC	2 cut(s)	328, 478
BpiI	GAAGAC	1 cut(s)	697
BplI	GAGNNNNNCTC	2 cut(s)	467, 499
BpmI	CTGGAG	1 cut(s)	398
BpuEI	CTTGAG	2 cut(s)	168, 191
BsaHI	GRCGYC	1 cut(s)	174
BsaI	GGTCTC	1 cut(s)	374
BsaJI	CCNNGG	1 cut(s)	321
BsaXI	ACNNNNNCTCC	2 cut(s)	293, 323
BseDI	CCNNGG	1 cut(s)	321
BseMII	CTCAG	1 cut(s)	156
BseXI	GCAGC	4 cut(s)	16, 120, 187, 458
BshFI	GGCC	4 cut(s)	24, 224, 276, 461
BsmAI	GTCTC	1 cut(s)	374
BsmI	GAATGC	1 cut(s)	643
BsnI	GGCC	4 cut(s)	24, 224, 276, 461
Bso31I	GGTCTC	1 cut(s)	374
Bsp1286I	GDGCHC	1 cut(s)	196
Bsp143I	GATC	5 cut(s)	13, 76, 366, 604, 680
BspACI	CCGC	4 cut(s)	9, 481, 594, 706
BspANI	GGCC	4 cut(s)	24, 224, 276, 461
BspCNI	CTCAG	1 cut(s)	157
BspQI	GCTCTTC	1 cut(s)	118
BspTNI	GGTCTC	1 cut(s)	374
BsrBI	CCGCTC	1 cut(s)	483
BssECI	CCNNGG	1 cut(s)	321
BssMI	GATC	5 cut(s)	13, 76, 366, 604, 680
BssNI	GRCGYC	1 cut(s)	174
BssT1I	CCWWGG	1 cut(s)	321
Bst4CI	ACNGT	1 cut(s)	412
Bst6I	CTCTTC	2 cut(s)	118, 224
BstACI	GRCGYC	1 cut(s)	174
BstC8I	GCNNGC	1 cut(s)	9
BstDEI	CTNAG	1 cut(s)	165
BstKTI	GATC	5 cut(s)	16, 79, 369, 607, 683
BstMAI	GTCTC	1 cut(s)	374
BstMBI	GATC	5 cut(s)	13, 76, 366, 604, 680
BstMWI	GCNNNNNNNGC	4 cut(s)	108, 200, 480, 551
BstV1I	GCAGC	4 cut(s)	16, 120, 187, 458
BstV2I	GAAGAC	1 cut(s)	697
BsuRI	GGCC	4 cut(s)	24, 224, 276, 461
BtgZI	GCGATG	1 cut(s)	453
BtsIMutI	CAGTG	1 cut(s)	408
Cac8I	GCNNGC	1 cut(s)	9
Cfr13I	GGNCC	2 cut(s)	328, 478
CseI	GACGC	1 cut(s)	182
Csp6I	GTAC	2 cut(s)	162, 413
CviAII	CATG	1 cut(s)	170
CviQI	GTAC	2 cut(s)	162, 413
DdeI	CTNAG	1 cut(s)	165
DpnI	GATC	5 cut(s)	15, 78, 368, 606, 682
DpnII	GATC	5 cut(s)	13, 76, 366, 604, 680
Eam1104I	CTCTTC	2 cut(s)	118, 224
EarI	CTCTTC	2 cut(s)	118, 224
Eco130I	CCWWGG	1 cut(s)	321
Eco147I	AGGCCT	2 cut(s)	276, 461
Eco24I	GRGCYC	1 cut(s)	196
Eco31I	GGTCTC	1 cut(s)	374
Eco47I	GGWCC	2 cut(s)	328, 478
EcoRI	GAATTC	1 cut(s)	570
EcoT14I	CCWWGG	1 cut(s)	321
EcoT38I	GRGCYC	1 cut(s)	196
ErhI	CCWWGG	1 cut(s)	321
FaeI	CATG	1 cut(s)	173
FaiI	YATR	4 cut(s)	95, 129, 171, 432
FatI	CATG	1 cut(s)	169
FauI	CCCGC	1 cut(s)	16
FbaI	TGATCA	2 cut(s)	13, 76
Fnu4HI	GCNGC	5 cut(s)	5, 109, 201, 472, 594
FriOI	GRGCYC	1 cut(s)	196
Fsp4HI	GCNGC	5 cut(s)	5, 109, 201, 472, 594
FspBI	CTAG	1 cut(s)	672
GluI	GCNGC	5 cut(s)	5, 109, 201, 472, 594
GsuI	CTGGAG	1 cut(s)	398
HaeIII	GGCC	4 cut(s)	24, 224, 276, 461
HgaI	GACGC	1 cut(s)	182
Hin1I	GRCGYC	1 cut(s)	174
Hin1II	CATG	1 cut(s)	173
HindIII	AAGCTT	2 cut(s)	100, 266
HinfI	GANTC	3 cut(s)	211, 246, 617
HphI	GGTGA	1 cut(s)	171
Hpy188I	TCNGA	3 cut(s)	364, 394, 487
Hpy188III	TCNNGA	5 cut(s)	143, 185, 208, 260, 331
HpyAV	CCTTC	4 cut(s)	228, 287, 394, 399
HpyCH4III	ACNGT	1 cut(s)	412
HpyCH4IV	ACGT	3 cut(s)	66, 359, 579
HpyCH4V	TGCA	5 cut(s)	4, 97, 108, 288, 634
HpyF10VI	GCNNNNNNNGC	4 cut(s)	108, 200, 480, 551
HpyF3I	CTNAG	1 cut(s)	165
HpySE526I	ACGT	3 cut(s)	66, 359, 579
Hsp92I	GRCGYC	1 cut(s)	174
Hsp92II	CATG	1 cut(s)	173
Ksp22I	TGATCA	2 cut(s)	13, 76
Kzo9I	GATC	5 cut(s)	13, 76, 366, 604, 680
LguI	GCTCTTC	1 cut(s)	118
LmnI	GCTCC	2 cut(s)	488, 668
LpnPI	CCDG	6 cut(s)	128, 138, 326, 344, 362, 510
Lsp1109I	GCAGC	4 cut(s)	16, 120, 187, 458
MaeI	CTAG	1 cut(s)	672
MaeII	ACGT	3 cut(s)	66, 359, 579
MalI	GATC	5 cut(s)	15, 78, 368, 606, 682
MbiI	CCGCTC	1 cut(s)	483
MboI	GATC	5 cut(s)	13, 76, 366, 604, 680
MboII	GAAGA	8 cut(s)	105, 108, 241, 272, 411, 456, 579, 702
MhlI	GDGCHC	1 cut(s)	196
MluCI	AATT	6 cut(s)	124, 289, 447, 504, 549, 570
MmeI	TCCRAC	2 cut(s)	510, 690
MseI	TTAA	1 cut(s)	59
MspA1I	CMGCKG	1 cut(s)	11
Mva1269I	GAATGC	1 cut(s)	643
MwoI	GCNNNNNNNGC	4 cut(s)	108, 200, 480, 551
NdeII	GATC	5 cut(s)	13, 76, 366, 604, 680
NlaIII	CATG	1 cut(s)	173
NmeAIII	GCCGAG	1 cut(s)	250
PceI	AGGCCT	2 cut(s)	276, 461
PciSI	GCTCTTC	1 cut(s)	118
PcsI	WCGNNNNNNNCGW	1 cut(s)	585
PctI	GAATGC	1 cut(s)	643
PfeI	GAWTC	3 cut(s)	211, 246, 617
PkrI	GCNGC	5 cut(s)	6, 110, 202, 473, 595
PspPI	GGNCC	2 cut(s)	328, 478
RsaI	GTAC	2 cut(s)	163, 414
RsaNI	GTAC	2 cut(s)	162, 413
SapI	GCTCTTC	1 cut(s)	118
SaqAI	TTAA	1 cut(s)	59
SatI	GCNGC	5 cut(s)	5, 109, 201, 472, 594
Sau3AI	GATC	5 cut(s)	13, 76, 366, 604, 680
Sau96I	GGNCC	2 cut(s)	328, 478
ScaI	AGTACT	1 cut(s)	163
SduI	GDGCHC	1 cut(s)	196
SinI	GGWCC	2 cut(s)	328, 478
SmlI	CTYRAG	2 cut(s)	183, 206
SmoI	CTYRAG	2 cut(s)	183, 206
Sse9I	AATT	6 cut(s)	124, 289, 447, 504, 549, 570
SseBI	AGGCCT	2 cut(s)	276, 461
SsiI	CCGC	4 cut(s)	9, 481, 594, 706
SspMI	CTAG	1 cut(s)	672
StuI	AGGCCT	2 cut(s)	276, 461
StyI	CCWWGG	1 cut(s)	321
TaaI	ACNGT	1 cut(s)	412
TaiI	ACGT	3 cut(s)	69, 362, 582
TaqI	TCGA	2 cut(s)	261, 271
TasI	AATT	6 cut(s)	124, 289, 447, 504, 549, 570
TatI	WGTACW	2 cut(s)	161, 412
TauI	GCSGC	1 cut(s)	596
TfiI	GAWTC	3 cut(s)	211, 246, 617
Tru1I	TTAA	1 cut(s)	59
Tru9I	TTAA	1 cut(s)	59
TscAI	CASTG	1 cut(s)	415
TseI	GCWGC	4 cut(s)	4, 108, 200, 471
TspDTI	ATGAA	1 cut(s)	417
TspRI	CASTG	1 cut(s)	415
VpaK11BI	GGWCC	2 cut(s)	328, 478
XapI	RAATTY	1 cut(s)	570
XspI	CTAG	1 cut(s)	672
ZrmI	AGTACT	1 cut(s)	163

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.