Rh5DG364700
BHLH Family

Transcription factor bHLH106-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0012151
Physical Location & Seq
Forward (+)
54603921 .. 54605807
1887 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG364700.1

Sequence Viewer

Length: 792 bp
ATGCAGCCTGAAAACCAAGAACTCTACCGCTTTCTTGCCCAAAACGGCTTAATCAACGGTCATCCCTATGGATCATTTCCCGGCGGAGTAGCTAATTGTGAGTTCTCATCTTCAGCTTCAGCTGTTCCTATGCAGCACAGCTTTTGCAGCTCCTCCTCTTATTACAGTAATAATTACCCCTTGAATAATCTGGAGGGGTCTGGGGTACTCAGTACTACTAGTACTGATACTACTACTACACCTCACGATAGAGCCCTTGCTGCTCTCAACAATCACAAGGAGGCCGAGAAGAGAAGGAGAGAGAGAATTAACTCACACCTCGATAAGCTTCGGGCACTTCTTCCTTGTAATTCCAAGACAGACAAAGCCTCGCTGTTAGCAAAGGTGGTTGAACGAGTGAAAGAGCTGAAAAACCAAATGTCAGAAATCACAGAACTCGAAACGTTCCCTGCTGAAACTGATGAAATTACTGTACTTTCCTCGGGTGAGGATGATTATTCGAGCAATGGAGGAATTATTTTCAAAGCATCATTGTCCTGCGAGGACCGACCAGAACTCCTATCCGATGTAATTGAAATACTAAAATCCCTCCATTTGAAGACACTTAAAGCTGAAATGGCAACTCTCGGAGGAAGAATCCGCAGTGTACTTATTGTTGCCGCGGATAAAGATCACACAATTGAATCAGTTCCCTTCTTGCAGAATGCATTCAAGTCTTTATTAGAACGCTCTAACTCTAGTGAGAGATCAAAAAGGCGTCGCCGTGTATTAGATCCAACGTTACTCGTCTAG

Protein Analysis

263

Amino Acids

29.14

Weight (kDa)

6.4

Isoelectric Point (pI)

51.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 90 - 137 8.3e-12 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012151)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41130 AT3G56770
fragaria_vesca FvH4_3g28400
malus_domestica MD12G1095300.v1.1 MD14G1091000.v1.1
prunus_persica Prupe.7G031500_v2.0.a1
pyrus_communis pycom12g08210 pycom14g08270
rosa_chinensis RchiOBHm_Chr5g0053301
rosa_laevigata RLG00000034769
rosa_multiflora Rmu_co8065512.1_g000001 Rmu_sc0001966.1_g000015
rosa_roxburghii Rroxscaffold_1G00029060
rosa_rugosa Rorug05G0267500
rosa_samantha Rh5AG340100 Rh5BG350800 Rh5CG376900 Rh5DG364700
rosa_wichuraiana Rw5G032110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 662
AciI CCGC 5 cut(s) 28, 84, 640, 660, 662
AclI AACGTT 2 cut(s) 443, 779
AclWI GGATC 2 cut(s) 79, 767
AcuI CTGAAG 2 cut(s) 96, 102
AcyI GRCGYC 1 cut(s) 757
AfaI GTAC 5 cut(s) 207, 214, 223, 474, 648
AgsI TTSAA 7 cut(s) 184, 392, 523, 575, 598, 683, 712
AhlI ACTAGT 1 cut(s) 218
AluBI AGCT 8 cut(s) 92, 116, 122, 141, 150, 328, 406, 611
AluI AGCT 8 cut(s) 92, 116, 122, 141, 150, 328, 406, 611
AlwI GGATC 2 cut(s) 79, 767
Ama87I CYCGRG 1 cut(s) 481
AoxI GGCC 1 cut(s) 282
ApeKI GCWGC 4 cut(s) 4, 133, 147, 260
Asp700I GAANNNNTTC 2 cut(s) 687, 707
AspS9I GGNCC 1 cut(s) 544
AsuC2I CCSGG 1 cut(s) 81
AsuHPI GGTGA 1 cut(s) 497
AvaI CYCGRG 1 cut(s) 481
AvaII GGWCC 1 cut(s) 544
BaeGI GKGCMC 1 cut(s) 337
BanII GRGCYC 1 cut(s) 256
BbsI GAAGAC 1 cut(s) 605
BbvI GCAGC 4 cut(s) 16, 145, 159, 247
BceAI ACGGC 2 cut(s) 61, 747
BcnI CCSGG 1 cut(s) 81
BcuI ACTAGT 1 cut(s) 218
BfaI CTAG 3 cut(s) 219, 738, 790
BisI GCNGC 5 cut(s) 5, 134, 148, 261, 660
BlsI GCNGC 5 cut(s) 6, 135, 149, 262, 661
BmcAI AGTACT 2 cut(s) 214, 223
Bme1390I CCNGG 1 cut(s) 81
Bme18I GGWCC 1 cut(s) 544
BmeT110I CYCGRG 1 cut(s) 481
BmgT120I GGNCC 1 cut(s) 544
BmrFI CCNGG 1 cut(s) 81
BmsI GCATC 1 cut(s) 536
BpiI GAAGAC 1 cut(s) 605
BpmI CTGGAG 1 cut(s) 212
BpuMI CCSGG 1 cut(s) 81
BsaBI GATNNNNATC 1 cut(s) 669
BsaHI GRCGYC 1 cut(s) 757
BsaJI CCNNGG 2 cut(s) 480, 660
BsaXI ACNNNNNCTCC 2 cut(s) 540, 570
Bse3DI GCAATG 1 cut(s) 511
Bse8I GATNNNNATC 1 cut(s) 669
BseDI CCNNGG 2 cut(s) 480, 660
BseGI GGATG 2 cut(s) 61, 496
BseJI GATNNNNATC 1 cut(s) 669
BseMI GCAATG 1 cut(s) 511
BseMII CTCAG 1 cut(s) 223
BseRI GAGGAG 2 cut(s) 142, 145
BseSI GKGCMC 1 cut(s) 337
BseXI GCAGC 4 cut(s) 16, 145, 159, 247
Bsh1236I CGCG 1 cut(s) 662
BshFI GGCC 1 cut(s) 284
BsiHKCI CYCGRG 1 cut(s) 481
BsiSI CCGG 1 cut(s) 81
BsmI GAATGC 2 cut(s) 707, 709
BsnI GGCC 1 cut(s) 284
BsoBI CYCGRG 1 cut(s) 481
Bsp1286I GDGCHC 2 cut(s) 256, 337
Bsp143I GATC 4 cut(s) 71, 670, 746, 772
BspACI CCGC 5 cut(s) 28, 84, 640, 660, 662
BspANI GGCC 1 cut(s) 284
BspCNI CTCAG 1 cut(s) 222
BspFNI CGCG 1 cut(s) 662
BspPI GGATC 2 cut(s) 79, 767
BsrDI GCAATG 1 cut(s) 511
BssECI CCNNGG 2 cut(s) 480, 660
BssMI GATC 4 cut(s) 71, 670, 746, 772
BssNI GRCGYC 1 cut(s) 757
Bst4CI ACNGT 3 cut(s) 59, 167, 472
Bst6I CTCTTC 1 cut(s) 284
BstACI GRCGYC 1 cut(s) 757
BstDEI CTNAG 1 cut(s) 209
BstDSI CCRYGG 1 cut(s) 660
BstF5I GGATG 2 cut(s) 61, 496
BstFNI CGCG 1 cut(s) 662
BstKTI GATC 4 cut(s) 74, 673, 749, 775
BstMBI GATC 4 cut(s) 71, 670, 746, 772
BstMWI GCNNNNNNNGC 3 cut(s) 147, 260, 617
BstSCI CCNGG 1 cut(s) 79
BstSLI GKGCMC 1 cut(s) 337
BstUI CGCG 1 cut(s) 662
BstV1I GCAGC 4 cut(s) 16, 145, 159, 247
BstV2I GAAGAC 1 cut(s) 605
BstX2I RGATCY 1 cut(s) 772
BstYI RGATCY 1 cut(s) 772
BsuRI GGCC 1 cut(s) 284
BtgI CCRYGG 1 cut(s) 660
BtsCI GGATG 2 cut(s) 61, 496
BtsI GCAGTG 1 cut(s) 649
BtsIMutI CAGTG 1 cut(s) 649
Cfr13I GGNCC 1 cut(s) 544
Cfr42I CCGCGG 1 cut(s) 663
CseI GACGC 1 cut(s) 746
Csp6I GTAC 5 cut(s) 206, 213, 222, 473, 647
CviQI GTAC 5 cut(s) 206, 213, 222, 473, 647
DdeI CTNAG 1 cut(s) 209
DpnI GATC 4 cut(s) 73, 672, 748, 774
DpnII GATC 4 cut(s) 71, 670, 746, 772
Eam1104I CTCTTC 1 cut(s) 284
EarI CTCTTC 1 cut(s) 284
EciI GGCGGA 1 cut(s) 99
Eco24I GRGCYC 1 cut(s) 256
Eco47I GGWCC 1 cut(s) 544
Eco57I CTGAAG 2 cut(s) 96, 102
Eco88I CYCGRG 1 cut(s) 481
EcoT22I ATGCAT 1 cut(s) 709
EcoT38I GRGCYC 1 cut(s) 256
FaiI YATR 2 cut(s) 69, 131
Fnu4HI GCNGC 5 cut(s) 5, 134, 148, 261, 660
FokI GGATG 2 cut(s) 48, 503
FriOI GRGCYC 1 cut(s) 256
Fsp4HI GCNGC 5 cut(s) 5, 134, 148, 261, 660
FspBI CTAG 3 cut(s) 219, 738, 790
GluI GCNGC 5 cut(s) 5, 134, 148, 261, 660
GsuI CTGGAG 1 cut(s) 212
HaeIII GGCC 1 cut(s) 284
HapII CCGG 1 cut(s) 81
HgaI GACGC 1 cut(s) 746
Hin1I GRCGYC 1 cut(s) 757
HindIII AAGCTT 1 cut(s) 326
HinfI GANTC 2 cut(s) 636, 683
HpaII CCGG 1 cut(s) 81
HphI GGTGA 1 cut(s) 497
Hpy166II GTNNAC 1 cut(s) 647
Hpy188I TCNGA 3 cut(s) 424, 565, 629
Hpy188III TCNNGA 2 cut(s) 191, 245
Hpy8I GTNNAC 1 cut(s) 647
Hpy99I CGWCG 1 cut(s) 762
HpyAV CCTTC 2 cut(s) 288, 703
HpyCH4III ACNGT 3 cut(s) 59, 167, 472
HpyCH4IV ACGT 2 cut(s) 443, 779
HpyCH4V TGCA 5 cut(s) 4, 133, 147, 700, 707
HpyF10VI GCNNNNNNNGC 3 cut(s) 147, 260, 617
HpyF3I CTNAG 1 cut(s) 209
HpySE526I ACGT 2 cut(s) 443, 779
Hsp92I GRCGYC 1 cut(s) 757
KspI CCGCGG 1 cut(s) 663
Kzo9I GATC 4 cut(s) 71, 670, 746, 772
LmnI GCTCC 1 cut(s) 155
LpnPI CCDG 7 cut(s) 21, 94, 176, 186, 462, 550, 564
Lsp1109I GCAGC 4 cut(s) 16, 145, 159, 247
LweI GCATC 1 cut(s) 536
MaeI CTAG 3 cut(s) 219, 738, 790
MaeII ACGT 2 cut(s) 443, 779
MaeIII GTNAC 1 cut(s) 780
MalI GATC 4 cut(s) 73, 672, 748, 774
MboI GATC 4 cut(s) 71, 670, 746, 772
MboII GAAGA 5 cut(s) 102, 301, 332, 610, 645
MfeI CAATTG 1 cut(s) 678
MflI RGATCY 1 cut(s) 772
MhlI GDGCHC 2 cut(s) 256, 337
MluCI AATT 8 cut(s) 94, 172, 306, 349, 465, 513, 570, 678
Mph1103I ATGCAT 1 cut(s) 709
MroXI GAANNNNTTC 2 cut(s) 687, 707
MseI TTAA 3 cut(s) 50, 309, 606
MslI CAYNNNNRTG 1 cut(s) 66
MspA1I CMGCKG 2 cut(s) 122, 662
MspI CCGG 1 cut(s) 81
MspR9I CCNGG 1 cut(s) 81
MunI CAATTG 1 cut(s) 678
Mva1269I GAATGC 2 cut(s) 707, 709
MvnI CGCG 1 cut(s) 662
MwoI GCNNNNNNNGC 3 cut(s) 147, 260, 617
NciI CCSGG 1 cut(s) 81
NdeII GATC 4 cut(s) 71, 670, 746, 772
NmeAIII GCCGAG 1 cut(s) 310
NsiI ATGCAT 1 cut(s) 709
PctI GAATGC 2 cut(s) 707, 709
PdmI GAANNNNTTC 2 cut(s) 687, 707
PfeI GAWTC 2 cut(s) 636, 683
PkrI GCNGC 5 cut(s) 6, 135, 149, 262, 661
Psp1406I AACGTT 2 cut(s) 443, 779
PspPI GGNCC 1 cut(s) 544
PsuI RGATCY 1 cut(s) 772
PvuII CAGCTG 1 cut(s) 122
RsaI GTAC 5 cut(s) 207, 214, 223, 474, 648
RsaNI GTAC 5 cut(s) 206, 213, 222, 473, 647
RseI CAYNNNNRTG 1 cut(s) 66
SacII CCGCGG 1 cut(s) 663
SaqAI TTAA 3 cut(s) 50, 309, 606
SatI GCNGC 5 cut(s) 5, 134, 148, 261, 660
Sau3AI GATC 4 cut(s) 71, 670, 746, 772
Sau96I GGNCC 1 cut(s) 544
ScaI AGTACT 2 cut(s) 214, 223
ScrFI CCNGG 1 cut(s) 81
SduI GDGCHC 2 cut(s) 256, 337
SfaNI GCATC 1 cut(s) 536
Sfr303I CCGCGG 1 cut(s) 663
SgrBI CCGCGG 1 cut(s) 663
SinI GGWCC 1 cut(s) 544
SmiMI CAYNNNNRTG 1 cut(s) 66
SpeI ACTAGT 1 cut(s) 218
Sse9I AATT 8 cut(s) 94, 172, 306, 349, 465, 513, 570, 678
SsiI CCGC 5 cut(s) 28, 84, 640, 660, 662
SspMI CTAG 3 cut(s) 219, 738, 790
StyD4I CCNGG 1 cut(s) 79
TaaI ACNGT 3 cut(s) 59, 167, 472
TaiI ACGT 2 cut(s) 446, 782
TaqI TCGA 3 cut(s) 321, 438, 500
TaqII GACCGA 1 cut(s) 561
TasI AATT 8 cut(s) 94, 172, 306, 349, 465, 513, 570, 678
TatI WGTACW 4 cut(s) 212, 221, 472, 646
TauI GCSGC 1 cut(s) 662
TfiI GAWTC 2 cut(s) 636, 683
Tru1I TTAA 3 cut(s) 50, 309, 606
Tru9I TTAA 3 cut(s) 50, 309, 606
TscAI CASTG 1 cut(s) 649
TseI GCWGC 4 cut(s) 4, 133, 147, 260
TspDTI ATGAA 1 cut(s) 477
TspRI CASTG 1 cut(s) 649
VpaK11BI GGWCC 1 cut(s) 544
XmnI GAANNNNTTC 2 cut(s) 687, 707
XspI CTAG 3 cut(s) 219, 738, 790
ZrmI AGTACT 2 cut(s) 214, 223
Zsp2I ATGCAT 1 cut(s) 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.