MD12G1102700.v1.1

Pistil-specific extensin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0015362
Physical Location & Seq
Forward (+)
16063908 .. 16065436
1529 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1102700.v1.1.491

Sequence Viewer

Length: 534 bp
ATGAGAACCATGGTGGTGCTGATATTTGCAGTGGTGCTGATTGCAATGGGTTGCAGCAGCATCCACACAGCTGAAGCTTCTAATTATGAAGAACCCAAAATCAAAACCATACATGTAGGTGGCAAGGTACTATGCCAAGATTGCACCCAAGGTTGGAACGAATGGGTTCACGGTGCCAGACCCATCAAGGGTGGGAAGGTGTCAGTAACATGCATGGATGACAGAAGCAGGGTTGCTTTCTACGGAAGCGATTTGACGGACGAGAAAGGCCAATTCGACCTGATCGTGAACAAGTACACGATCAACGACAAAGAAGTGAAAGCCAGTCGTTGCTCAGTGAGGTTGGTATCTTGCCCAGATGCCACGTGTAACGTTCCGACTAATTTCGGCAGAGGCCGGTCAGGGATGAAGTTGAACCGGCCGAGTTTTGTGTACAGAGATTTGGTGAAGTACACTCTTGGTCCATTCTATTACACCAGTCCTATGTGTGAAAAATTTGACACTACTCATCCTGATCAGTCTAATAAAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

178

Amino Acids

19.75

Weight (kDa)

8.76

Isoelectric Point (pI)

31.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 39 - 135 8.5e-25 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09925
fragaria_vesca FvH4_6g17250
malus_domestica MD12G1102700.v1.1 MD14G1096900.v1.1
prunus_persica Prupe.7G122800_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0471351
rosa_laevigata RLG00000024184
rosa_multiflora Rmu_sc0006582.1_g000024 Rmu_sc0010513.1_g000008
rosa_roxburghii Rroxscaffold_6G00410040
rosa_rugosa Rorug03G0117300
rosa_samantha Rh3AG166900 Rh3BG192300 Rh3CG182600 Rh3DG183500
rosa_wichuraiana Rw3G015700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 173
AclI AACGTT 1 cut(s) 372
AcoI YGGCCR 1 cut(s) 419
AcsI RAATTY 1 cut(s) 494
AcuI CTGAAG 1 cut(s) 93
AcvI CACGTG 1 cut(s) 366
AfaI GTAC 4 cut(s) 129, 296, 434, 452
AfiI CCNNNNNNNGG 2 cut(s) 153, 188
AflIII ACRYGT 2 cut(s) 112, 365
AgsI TTSAA 1 cut(s) 415
AluBI AGCT 2 cut(s) 71, 77
AluI AGCT 2 cut(s) 71, 77
AoxI GGCC 3 cut(s) 268, 394, 419
ApeKI GCWGC 2 cut(s) 54, 57
ApoI RAATTY 1 cut(s) 494
Asp700I GAANNNNTTC 1 cut(s) 165
AspS9I GGNCC 1 cut(s) 461
AsuHPI GGTGA 1 cut(s) 457
AvaII GGWCC 1 cut(s) 461
BanI GGYRCC 1 cut(s) 173
BbrPI CACGTG 1 cut(s) 366
BbvI GCAGC 2 cut(s) 66, 69
BccI CCATC 1 cut(s) 191
BclI TGATCA 1 cut(s) 514
BisI GCNGC 2 cut(s) 55, 58
BlsI GCNGC 2 cut(s) 56, 59
Bme18I GGWCC 1 cut(s) 461
BmgT120I GGNCC 1 cut(s) 461
BmiI GGNNCC 1 cut(s) 175
BmsI GCATC 2 cut(s) 69, 349
BsaAI YACGTR 1 cut(s) 366
BsaJI CCNNGG 2 cut(s) 9, 148
Bsc4I CCNNNNNNNGG 2 cut(s) 153, 188
Bse118I RCCGGY 2 cut(s) 396, 417
Bse1I ACTGG 2 cut(s) 324, 477
Bse3DI GCAATG 1 cut(s) 51
BseDI CCNNGG 2 cut(s) 9, 148
BseGI GGATG 4 cut(s) 60, 223, 411, 508
BseLI CCNNNNNNNGG 2 cut(s) 153, 188
BseMI GCAATG 1 cut(s) 51
BseMII CTCAG 1 cut(s) 348
BseNI ACTGG 2 cut(s) 324, 477
BseX3I CGGCCG 1 cut(s) 419
BseXI GCAGC 2 cut(s) 66, 69
Bsh1285I CGRYCG 1 cut(s) 422
BshFI GGCC 3 cut(s) 270, 396, 421
BshNI GGYRCC 1 cut(s) 173
BsiEI CGRYCG 1 cut(s) 422
BsiSI CCGG 2 cut(s) 397, 418
BslI CCNNNNNNNGG 2 cut(s) 153, 188
BsnI GGCC 3 cut(s) 270, 396, 421
Bsp1407I TGTACA 1 cut(s) 432
Bsp143I GATC 3 cut(s) 282, 300, 514
Bsp19I CCATGG 1 cut(s) 9
BspANI GGCC 3 cut(s) 270, 396, 421
BspCNI CTCAG 1 cut(s) 347
BspLI GGNNCC 1 cut(s) 175
BspT107I GGYRCC 1 cut(s) 173
BsrDI GCAATG 1 cut(s) 51
BsrFI RCCGGY 2 cut(s) 396, 417
BsrGI TGTACA 1 cut(s) 432
BsrI ACTGG 2 cut(s) 324, 477
BssAI RCCGGY 2 cut(s) 396, 417
BssECI CCNNGG 2 cut(s) 9, 148
BssMI GATC 3 cut(s) 282, 300, 514
BssT1I CCWWGG 2 cut(s) 9, 148
Bst4CI ACNGT 1 cut(s) 173
BstAUI TGTACA 1 cut(s) 432
BstBAI YACGTR 1 cut(s) 366
BstDEI CTNAG 1 cut(s) 334
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 4 cut(s) 60, 223, 411, 508
BstKTI GATC 3 cut(s) 285, 303, 517
BstMBI GATC 3 cut(s) 282, 300, 514
BstMCI CGRYCG 1 cut(s) 422
BstMWI GCNNNNNNNGC 1 cut(s) 141
BstNSI RCATGY 2 cut(s) 116, 213
BstV1I GCAGC 2 cut(s) 66, 69
BstZI CGGCCG 1 cut(s) 419
BsuRI GGCC 3 cut(s) 270, 396, 421
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 4 cut(s) 60, 223, 411, 508
BtsI GCAGTG 1 cut(s) 36
BtsIMutI CAGTG 2 cut(s) 36, 342
Cfr10I RCCGGY 2 cut(s) 396, 417
Cfr13I GGNCC 1 cut(s) 461
Csp6I GTAC 4 cut(s) 128, 295, 433, 451
CviAII CATG 4 cut(s) 10, 113, 210, 214
CviJI RGCY 6 cut(s) 71, 77, 270, 323, 396, 421
CviKI_1 RGCY 6 cut(s) 71, 77, 270, 323, 396, 421
CviQI GTAC 4 cut(s) 128, 295, 433, 451
DdeI CTNAG 1 cut(s) 334
DpnI GATC 3 cut(s) 284, 302, 516
DpnII GATC 3 cut(s) 282, 300, 514
EaeI YGGCCR 1 cut(s) 419
EagI CGGCCG 1 cut(s) 419
EclXI CGGCCG 1 cut(s) 419
Eco130I CCWWGG 2 cut(s) 9, 148
Eco47I GGWCC 1 cut(s) 461
Eco52I CGGCCG 1 cut(s) 419
Eco57I CTGAAG 1 cut(s) 93
Eco72I CACGTG 1 cut(s) 366
EcoT14I CCWWGG 2 cut(s) 9, 148
EcoT22I ATGCAT 1 cut(s) 215
ErhI CCWWGG 2 cut(s) 9, 148
FaeI CATG 4 cut(s) 13, 116, 213, 217
FaiI YATR 8 cut(s) 11, 87, 110, 114, 133, 211, 215, 485
FatI CATG 4 cut(s) 9, 112, 209, 213
FbaI TGATCA 1 cut(s) 514
Fnu4HI GCNGC 2 cut(s) 55, 58
FokI GGATG 4 cut(s) 47, 230, 418, 495
Fsp4HI GCNGC 2 cut(s) 55, 58
GluI GCNGC 2 cut(s) 55, 58
HaeIII GGCC 3 cut(s) 270, 396, 421
HapII CCGG 2 cut(s) 397, 418
Hin1II CATG 4 cut(s) 13, 116, 213, 217
HindIII AAGCTT 1 cut(s) 75
HpaII CCGG 2 cut(s) 397, 418
HphI GGTGA 1 cut(s) 457
Hpy166II GTNNAC 5 cut(s) 169, 289, 297, 433, 453
Hpy188I TCNGA 1 cut(s) 378
Hpy188III TCNNGA 2 cut(s) 286, 512
Hpy8I GTNNAC 5 cut(s) 169, 289, 297, 433, 453
HpyAV CCTTC 1 cut(s) 190
HpyCH4III ACNGT 1 cut(s) 173
HpyCH4IV ACGT 2 cut(s) 365, 372
HpyCH4V TGCA 5 cut(s) 29, 44, 54, 144, 213
HpyF10VI GCNNNNNNNGC 1 cut(s) 141
HpyF3I CTNAG 1 cut(s) 334
HpySE526I ACGT 2 cut(s) 365, 372
Hsp92II CATG 4 cut(s) 13, 116, 213, 217
Ksp22I TGATCA 1 cut(s) 514
Kzo9I GATC 3 cut(s) 282, 300, 514
Lsp1109I GCAGC 2 cut(s) 66, 69
LweI GCATC 2 cut(s) 69, 349
MaeII ACGT 2 cut(s) 365, 372
MaeIII GTNAC 2 cut(s) 205, 368
MalI GATC 3 cut(s) 284, 302, 516
MboI GATC 3 cut(s) 282, 300, 514
MboII GAAGA 1 cut(s) 101
MluCI AATT 4 cut(s) 82, 272, 382, 494
MmeI TCCRAC 2 cut(s) 134, 401
MnlI CCTC 2 cut(s) 333, 386
Mph1103I ATGCAT 1 cut(s) 215
MroXI GAANNNNTTC 1 cut(s) 165
MslI CAYNNNNRTG 2 cut(s) 14, 117
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 2 cut(s) 397, 418
MwoI GCNNNNNNNGC 1 cut(s) 141
NcoI CCATGG 1 cut(s) 9
NdeII GATC 3 cut(s) 282, 300, 514
NlaIII CATG 4 cut(s) 13, 116, 213, 217
NlaIV GGNNCC 1 cut(s) 175
NmeAIII GCCGAG 1 cut(s) 447
NsiI ATGCAT 1 cut(s) 215
NspI RCATGY 2 cut(s) 116, 213
PciI ACATGT 1 cut(s) 112
PcsI WCGNNNNNNNCGW 1 cut(s) 282
PdmI GAANNNNTTC 1 cut(s) 165
PkrI GCNGC 2 cut(s) 56, 59
PmaCI CACGTG 1 cut(s) 366
PmlI CACGTG 1 cut(s) 366
Ppu21I YACGTR 1 cut(s) 366
PscI ACATGT 1 cut(s) 112
Psp1406I AACGTT 1 cut(s) 372
PspCI CACGTG 1 cut(s) 366
PspN4I GGNNCC 1 cut(s) 175
PspPI GGNCC 1 cut(s) 461
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 4 cut(s) 129, 296, 434, 452
RsaNI GTAC 4 cut(s) 128, 295, 433, 451
RseI CAYNNNNRTG 2 cut(s) 14, 117
SatI GCNGC 2 cut(s) 55, 58
Sau3AI GATC 3 cut(s) 282, 300, 514
Sau96I GGNCC 1 cut(s) 461
SfaNI GCATC 2 cut(s) 69, 349
SinI GGWCC 1 cut(s) 461
SmiMI CAYNNNNRTG 2 cut(s) 14, 117
Sse9I AATT 4 cut(s) 82, 272, 382, 494
StyI CCWWGG 2 cut(s) 9, 148
TaaI ACNGT 1 cut(s) 173
TaiI ACGT 2 cut(s) 368, 375
TaqI TCGA 1 cut(s) 276
TasI AATT 4 cut(s) 82, 272, 382, 494
TatI WGTACW 3 cut(s) 294, 432, 450
TscAI CASTG 2 cut(s) 36, 342
TseI GCWGC 2 cut(s) 54, 57
TspDTI ATGAA 2 cut(s) 102, 422
TspGWI ACGGA 2 cut(s) 258, 272
TspRI CASTG 2 cut(s) 36, 342
VpaK11BI GGWCC 1 cut(s) 461
XapI RAATTY 1 cut(s) 494
XceI RCATGY 2 cut(s) 116, 213
XmnI GAANNNNTTC 1 cut(s) 165
Zsp2I ATGCAT 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.