MD14G1096900.v1.1

Pistil-specific extensin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015362
Physical Location & Seq
Forward (+)
14720301 .. 14722295
1995 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1096900.v1.1.491

Sequence Viewer

Length: 546 bp
ATGATGAGAGCCATGGTGGTGCTGTTATTTGCAGTGGTATTTATTGCCATGGGTTGCAGCAGCAGCCACACAGTTGAAGCTTTTAATTATGAAGAACCCAAAACCAAAACCATACATGTAGGTGGGAAAGTACTATGCCAAGATTGCACCCAAGGTTGGAACGAATGGGTTCACGGCGCCAGGCCCATCAATGGTGGGAAGGTGTCTATGACATGCATGGATGAGAGAAGCAGGGTTGTATTCTATGCAAGCGATCTGACAGACGAAAAAGGCCAATTCAACTTGATCATGAACAAGTACACAACCAACGGCAAAGAAGTGAATGCCAATCGATGCTCACTGAGGCTGGTGTCTTGCCCAGATGCCACGTGTAACGTTCCAACTAATTTCGGAGGAGGCCGGTCGGGAGTGAAGCTAAATCGGCCGAGTTTGGTGTATAGAGATTTGGTGAAGTACACTCTCGGTCCATTCTATTACACCAGTCCTATGTGCGAGAAACCTGACACTACTCGTTATGATGATCGTGGCAATGGACGTAACTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

182

Amino Acids

20.28

Weight (kDa)

8.77

Isoelectric Point (pI)

29.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 40 - 136 4.5e-23 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09925
fragaria_vesca FvH4_6g17250
malus_domestica MD12G1102700.v1.1 MD14G1096900.v1.1
prunus_persica Prupe.7G122800_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0471351
rosa_laevigata RLG00000024184
rosa_multiflora Rmu_sc0006582.1_g000024 Rmu_sc0010513.1_g000008
rosa_roxburghii Rroxscaffold_6G00410040
rosa_rugosa Rorug03G0117300
rosa_samantha Rh3AG166900 Rh3BG192300 Rh3CG182600 Rh3DG183500
rosa_wichuraiana Rw3G015700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 176
AclI AACGTT 1 cut(s) 375
AcoI YGGCCR 1 cut(s) 422
AcvI CACGTG 1 cut(s) 369
AcyI GRCGYC 1 cut(s) 177
AfaI GTAC 3 cut(s) 132, 299, 455
AfiI CCNNNNNNNGG 2 cut(s) 156, 191
AflIII ACRYGT 2 cut(s) 115, 368
AgsI TTSAA 2 cut(s) 77, 280
AjnI CCWGG 1 cut(s) 179
AluBI AGCT 2 cut(s) 80, 415
AluI AGCT 2 cut(s) 80, 415
AoxI GGCC 4 cut(s) 182, 271, 397, 422
ApeKI GCWGC 3 cut(s) 57, 60, 63
Asp700I GAANNNNTTC 1 cut(s) 168
AspLEI GCGC 1 cut(s) 179
AspS9I GGNCC 2 cut(s) 183, 464
AsuHPI GGTGA 1 cut(s) 460
AvaII GGWCC 1 cut(s) 464
BanI GGYRCC 1 cut(s) 176
BbrPI CACGTG 1 cut(s) 369
BbvI GCAGC 3 cut(s) 69, 72, 75
BccI CCATC 1 cut(s) 194
BceAI ACGGC 2 cut(s) 190, 325
BciT130I CCWGG 1 cut(s) 181
BclI TGATCA 1 cut(s) 285
BfoI RGCGCY 1 cut(s) 180
BisI GCNGC 3 cut(s) 58, 61, 64
BlsI GCNGC 3 cut(s) 59, 62, 65
BmcAI AGTACT 1 cut(s) 132
Bme1390I CCNGG 1 cut(s) 181
Bme18I GGWCC 1 cut(s) 464
BmgT120I GGNCC 2 cut(s) 183, 464
BmiI GGNNCC 1 cut(s) 178
BmrFI CCNGG 1 cut(s) 181
BmsI GCATC 2 cut(s) 323, 352
Bsa29I ATCGAT 1 cut(s) 331
BsaAI YACGTR 1 cut(s) 369
BsaHI GRCGYC 1 cut(s) 177
BsaJI CCNNGG 3 cut(s) 12, 48, 151
Bsc4I CCNNNNNNNGG 2 cut(s) 156, 191
Bse118I RCCGGY 1 cut(s) 399
Bse1I ACTGG 1 cut(s) 480
Bse3DI GCAATG 1 cut(s) 535
BseBI CCWGG 1 cut(s) 181
BseCI ATCGAT 1 cut(s) 331
BseDI CCNNGG 3 cut(s) 12, 48, 151
BseGI GGATG 1 cut(s) 226
BseLI CCNNNNNNNGG 2 cut(s) 156, 191
BseMI GCAATG 1 cut(s) 535
BseMII CTCAG 1 cut(s) 332
BseNI ACTGG 1 cut(s) 480
BseRI GAGGAG 1 cut(s) 408
BseX3I CGGCCG 1 cut(s) 422
BseXI GCAGC 3 cut(s) 69, 72, 75
Bsh1285I CGRYCG 2 cut(s) 404, 425
BshFI GGCC 4 cut(s) 184, 273, 399, 424
BshNI GGYRCC 1 cut(s) 176
BshVI ATCGAT 1 cut(s) 331
BsiEI CGRYCG 2 cut(s) 404, 425
BsiSI CCGG 1 cut(s) 400
BslI CCNNNNNNNGG 2 cut(s) 156, 191
BsmI GAATGC 1 cut(s) 328
BsnI GGCC 4 cut(s) 184, 273, 399, 424
Bsp143I GATC 3 cut(s) 253, 285, 520
Bsp19I CCATGG 2 cut(s) 12, 48
BspANI GGCC 4 cut(s) 184, 273, 399, 424
BspCNI CTCAG 1 cut(s) 333
BspDI ATCGAT 1 cut(s) 331
BspHI TCATGA 1 cut(s) 288
BspLI GGNNCC 1 cut(s) 178
BspT107I GGYRCC 1 cut(s) 176
BsrDI GCAATG 1 cut(s) 535
BsrFI RCCGGY 1 cut(s) 399
BsrI ACTGG 1 cut(s) 480
BssAI RCCGGY 1 cut(s) 399
BssECI CCNNGG 3 cut(s) 12, 48, 151
BssMI GATC 3 cut(s) 253, 285, 520
BssNI GRCGYC 1 cut(s) 177
BssT1I CCWWGG 3 cut(s) 12, 48, 151
Bst2UI CCWGG 1 cut(s) 181
Bst4CI ACNGT 1 cut(s) 73
BstACI GRCGYC 1 cut(s) 177
BstBAI YACGTR 1 cut(s) 369
BstC8I GCNNGC 1 cut(s) 250
BstDEI CTNAG 1 cut(s) 341
BstDSI CCRYGG 2 cut(s) 12, 48
BstF5I GGATG 1 cut(s) 226
BstH2I RGCGCY 1 cut(s) 180
BstHHI GCGC 1 cut(s) 179
BstKTI GATC 3 cut(s) 256, 288, 523
BstMBI GATC 3 cut(s) 253, 285, 520
BstMCI CGRYCG 2 cut(s) 404, 425
BstMWI GCNNNNNNNGC 3 cut(s) 63, 144, 421
BstNI CCWGG 1 cut(s) 181
BstNSI RCATGY 2 cut(s) 119, 216
BstSCI CCNGG 1 cut(s) 179
BstV1I GCAGC 3 cut(s) 69, 72, 75
BstZI CGGCCG 1 cut(s) 422
Bsu15I ATCGAT 1 cut(s) 331
BsuRI GGCC 4 cut(s) 184, 273, 399, 424
BsuTUI ATCGAT 1 cut(s) 331
BtgI CCRYGG 2 cut(s) 12, 48
BtsCI GGATG 1 cut(s) 226
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 2 cut(s) 39, 338
Cac8I GCNNGC 1 cut(s) 250
CciI TCATGA 1 cut(s) 288
CfoI GCGC 1 cut(s) 179
Cfr10I RCCGGY 1 cut(s) 399
Cfr13I GGNCC 2 cut(s) 183, 464
ClaI ATCGAT 1 cut(s) 331
Csp6I GTAC 3 cut(s) 131, 298, 454
CviAII CATG 6 cut(s) 13, 49, 116, 213, 217, 289
CviJI RGCY 9 cut(s) 11, 66, 80, 184, 273, 346, 399, 415, 424
CviKI_1 RGCY 9 cut(s) 11, 66, 80, 184, 273, 346, 399, 415, 424
CviQI GTAC 3 cut(s) 131, 298, 454
DdeI CTNAG 1 cut(s) 341
DinI GGCGCC 1 cut(s) 178
DpnI GATC 3 cut(s) 255, 287, 522
DpnII GATC 3 cut(s) 253, 285, 520
EaeI YGGCCR 1 cut(s) 422
EagI CGGCCG 1 cut(s) 422
EclXI CGGCCG 1 cut(s) 422
Eco130I CCWWGG 3 cut(s) 12, 48, 151
Eco47I GGWCC 1 cut(s) 464
Eco52I CGGCCG 1 cut(s) 422
Eco72I CACGTG 1 cut(s) 369
EcoRII CCWGG 1 cut(s) 179
EcoT14I CCWWGG 3 cut(s) 12, 48, 151
EcoT22I ATGCAT 1 cut(s) 218
EgeI GGCGCC 1 cut(s) 178
EheI GGCGCC 1 cut(s) 178
ErhI CCWWGG 3 cut(s) 12, 48, 151
FaeI CATG 6 cut(s) 16, 52, 119, 216, 220, 292
FatI CATG 6 cut(s) 12, 48, 115, 212, 216, 288
FbaI TGATCA 1 cut(s) 285
Fnu4HI GCNGC 3 cut(s) 58, 61, 64
FokI GGATG 1 cut(s) 233
Fsp4HI GCNGC 3 cut(s) 58, 61, 64
GlaI GCGC 1 cut(s) 178
GluI GCNGC 3 cut(s) 58, 61, 64
HaeII RGCGCY 1 cut(s) 180
HaeIII GGCC 4 cut(s) 184, 273, 399, 424
HapII CCGG 1 cut(s) 400
HhaI GCGC 1 cut(s) 179
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 6 cut(s) 16, 52, 119, 216, 220, 292
Hin6I GCGC 1 cut(s) 177
HinP1I GCGC 1 cut(s) 177
HindIII AAGCTT 1 cut(s) 78
HpaII CCGG 1 cut(s) 400
HphI GGTGA 1 cut(s) 460
Hpy166II GTNNAC 3 cut(s) 172, 300, 456
Hpy188I TCNGA 2 cut(s) 258, 392
Hpy188III TCNNGA 2 cut(s) 289, 405
Hpy8I GTNNAC 3 cut(s) 172, 300, 456
HpyAV CCTTC 1 cut(s) 193
HpyCH4III ACNGT 1 cut(s) 73
HpyCH4IV ACGT 3 cut(s) 368, 375, 535
HpyCH4V TGCA 5 cut(s) 32, 57, 147, 216, 248
HpyF10VI GCNNNNNNNGC 3 cut(s) 63, 144, 421
HpyF3I CTNAG 1 cut(s) 341
HpySE526I ACGT 3 cut(s) 368, 375, 535
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 6 cut(s) 16, 52, 119, 216, 220, 292
HspAI GCGC 1 cut(s) 177
KasI GGCGCC 1 cut(s) 176
Ksp22I TGATCA 1 cut(s) 285
Kzo9I GATC 3 cut(s) 253, 285, 520
LpnPI CCDG 8 cut(s) 166, 193, 217, 332, 372, 413, 493, 513
Lsp1109I GCAGC 3 cut(s) 69, 72, 75
LweI GCATC 2 cut(s) 323, 352
MaeII ACGT 3 cut(s) 368, 375, 535
MaeIII GTNAC 2 cut(s) 371, 536
MalI GATC 3 cut(s) 255, 287, 522
MboI GATC 3 cut(s) 253, 285, 520
MboII GAAGA 1 cut(s) 104
MluCI AATT 3 cut(s) 85, 275, 385
Mly113I GGCGCC 1 cut(s) 177
MmeI TCCRAC 2 cut(s) 137, 404
MnlI CCTC 3 cut(s) 336, 386, 389
Mph1103I ATGCAT 1 cut(s) 218
MroXI GAANNNNTTC 1 cut(s) 168
MseI TTAA 1 cut(s) 84
MslI CAYNNNNRTG 2 cut(s) 17, 120
MspI CCGG 1 cut(s) 400
MspR9I CCNGG 1 cut(s) 181
Mva1269I GAATGC 1 cut(s) 328
MvaI CCWGG 1 cut(s) 181
MwoI GCNNNNNNNGC 3 cut(s) 63, 144, 421
NarI GGCGCC 1 cut(s) 177
NcoI CCATGG 2 cut(s) 12, 48
NdeII GATC 3 cut(s) 253, 285, 520
NlaIII CATG 6 cut(s) 16, 52, 119, 216, 220, 292
NlaIV GGNNCC 1 cut(s) 178
NmeAIII GCCGAG 1 cut(s) 450
NsiI ATGCAT 1 cut(s) 218
NspI RCATGY 2 cut(s) 119, 216
PagI TCATGA 1 cut(s) 288
PciI ACATGT 1 cut(s) 115
PctI GAATGC 1 cut(s) 328
PdmI GAANNNNTTC 1 cut(s) 168
PkrI GCNGC 3 cut(s) 59, 62, 65
PluTI GGCGCC 1 cut(s) 180
PmaCI CACGTG 1 cut(s) 369
PmlI CACGTG 1 cut(s) 369
Ppu21I YACGTR 1 cut(s) 369
PscI ACATGT 1 cut(s) 115
Psp1406I AACGTT 1 cut(s) 375
Psp6I CCWGG 1 cut(s) 179
PspCI CACGTG 1 cut(s) 369
PspGI CCWGG 1 cut(s) 179
PspN4I GGNNCC 1 cut(s) 178
PspPI GGNCC 2 cut(s) 183, 464
RsaI GTAC 3 cut(s) 132, 299, 455
RsaNI GTAC 3 cut(s) 131, 298, 454
RseI CAYNNNNRTG 2 cut(s) 17, 120
SaqAI TTAA 1 cut(s) 84
SatI GCNGC 3 cut(s) 58, 61, 64
Sau3AI GATC 3 cut(s) 253, 285, 520
Sau96I GGNCC 2 cut(s) 183, 464
ScaI AGTACT 1 cut(s) 132
ScrFI CCNGG 1 cut(s) 181
SetI ASST 9 cut(s) 82, 124, 157, 204, 371, 378, 417, 502, 538
SfaNI GCATC 2 cut(s) 323, 352
SfoI GGCGCC 1 cut(s) 178
SinI GGWCC 1 cut(s) 464
SmiMI CAYNNNNRTG 2 cut(s) 17, 120
Sse9I AATT 3 cut(s) 85, 275, 385
SspDI GGCGCC 1 cut(s) 176
StyD4I CCNGG 1 cut(s) 179
StyI CCWWGG 3 cut(s) 12, 48, 151
TaaI ACNGT 1 cut(s) 73
TaiI ACGT 3 cut(s) 371, 378, 538
TaqI TCGA 1 cut(s) 331
TaqII GACCGA 1 cut(s) 452
TasI AATT 3 cut(s) 85, 275, 385
TatI WGTACW 3 cut(s) 130, 297, 453
Tru1I TTAA 1 cut(s) 84
Tru9I TTAA 1 cut(s) 84
TscAI CASTG 2 cut(s) 39, 345
TseI GCWGC 3 cut(s) 57, 60, 63
TspDTI ATGAA 2 cut(s) 105, 305
TspRI CASTG 2 cut(s) 39, 345
VpaK11BI GGWCC 1 cut(s) 464
XceI RCATGY 2 cut(s) 119, 216
XmnI GAANNNNTTC 1 cut(s) 168
ZrmI AGTACT 1 cut(s) 132
Zsp2I ATGCAT 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.