Rorug03G0117300

Pistil-specific extensin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000954
Physical Location & Seq
Reverse (-)
9526082 .. 9528871
2790 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0117300.1

Sequence Viewer

Length: 600 bp
ATGGATATACTTGTGCGTGGCTACGCTGGTTGGAATTCAAGGAATGGTCTGCAGGCTTTAGAGCAAGTGTTTCCAAAGGATGCAAGTATCCAGCCATCGTTGGTGATAGTCTATTTTGGTGGTAATGATTCAATGCTGCCGGATCCACTAGGCTTAAGCCTTCACGTACCTCTTCCTGAATATGTAGAAAATATGAGGAAGATTGCTACCCATTTGAAGAGCCTTTCGCAGAAAACCCGCCTTATCTTCCTGACTGCTCCTCCTGTCAACGAGGAACAAATGCGGAAAGCATTTAGTGGAATCATGCGGGAGAATGGTCGAACAAACAAGCAGTTGCAAATGTATTCAGATGCTTGTGTGGAGCTGTGCAGAGAGATGGATATCAAGGCAGTTGATCTCTTTACTTCAATGCAGAAAAGAAATGACTGGTCAACTGTATGCTTTACGGATGGAATCCATTTTTCATCCGAGGGGAGCAAGATCGTGGTGGAGGAGATACTGAAGGTCCTTGAAGAAGCAGACTGGGAACCCAGCCTGCAATGGAACTCATTGCCAACTGAGTTTGAAGAGTTTGCACCTTATACCACCGCTCGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

199

Amino Acids

22.65

Weight (kDa)

5.05

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL_2 PF13472 4 - 160 6.2e-18 GDSL-like Lipase/Acylhydrolase family
Lipase_GDSL PF00657 15 - 166 2.2e-14 GDSL-like Lipase/Acylhydrolase
DUF459 PF04311 30 - 168 2e-07 Protein of unknown function (DUF459)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 590
AciI CCGC 4 cut(s) 238, 283, 307, 588
AclWI GGATC 2 cut(s) 137, 150
AcsI RAATTY 1 cut(s) 34
AcuI CTGAAG 1 cut(s) 521
AfaI GTAC 1 cut(s) 168
AfiI CCNNNNNNNGG 1 cut(s) 591
AflII CTTAAG 1 cut(s) 154
AgsI TTSAA 6 cut(s) 39, 132, 217, 408, 512, 566
AluBI AGCT 1 cut(s) 364
AluI AGCT 1 cut(s) 364
AlwI GGATC 2 cut(s) 137, 150
Ama87I CYCGRG 1 cut(s) 591
ApeKI GCWGC 1 cut(s) 136
ApoI RAATTY 1 cut(s) 34
AspS9I GGNCC 1 cut(s) 505
AsuHPI GGTGA 1 cut(s) 115
AvaI CYCGRG 1 cut(s) 591
AvaII GGWCC 1 cut(s) 505
BamHI GGATCC 1 cut(s) 142
BbvI GCAGC 1 cut(s) 123
BccI CCATC 3 cut(s) 103, 370, 443
BciVI GTATCC 1 cut(s) 98
BfaI CTAG 1 cut(s) 149
BfmI CTRYAG 1 cut(s) 50
BfrI CTTAAG 1 cut(s) 154
BfuI GTATCC 1 cut(s) 98
BisI GCNGC 1 cut(s) 137
BlsI GCNGC 1 cut(s) 138
Bme18I GGWCC 1 cut(s) 505
BmeT110I CYCGRG 1 cut(s) 591
BmgT120I GGNCC 1 cut(s) 505
BmiI GGNNCC 2 cut(s) 144, 528
BmrI ACTGGG 1 cut(s) 532
BmsI GCATC 2 cut(s) 70, 340
BmuI ACTGGG 1 cut(s) 532
BsaAI YACGTR 1 cut(s) 166
BsaBI GATNNNNATC 1 cut(s) 380
BsaJI CCNNGG 1 cut(s) 468
BsaXI ACNNNNNCTCC 2 cut(s) 244, 274
Bsc4I CCNNNNNNNGG 1 cut(s) 591
Bse1I ACTGG 2 cut(s) 431, 527
Bse3DI GCAATG 2 cut(s) 545, 548
Bse8I GATNNNNATC 1 cut(s) 380
BseDI CCNNGG 1 cut(s) 468
BseGI GGATG 3 cut(s) 85, 454, 464
BseJI GATNNNNATC 1 cut(s) 380
BseLI CCNNNNNNNGG 1 cut(s) 591
BseMI GCAATG 2 cut(s) 545, 548
BseMII CTCAG 1 cut(s) 549
BseNI ACTGG 2 cut(s) 431, 527
BseRI GAGGAG 2 cut(s) 249, 506
BseXI GCAGC 1 cut(s) 123
BseYI CCCAGC 1 cut(s) 530
BsgI GTGCAG 1 cut(s) 388
BsiHKCI CYCGRG 1 cut(s) 591
BsiSI CCGG 1 cut(s) 140
BslI CCNNNNNNNGG 1 cut(s) 591
BsoBI CYCGRG 1 cut(s) 591
Bsp143I GATC 3 cut(s) 142, 394, 480
BspACI CCGC 4 cut(s) 238, 283, 307, 588
BspCNI CTCAG 1 cut(s) 550
BspLI GGNNCC 2 cut(s) 144, 528
BspMAI CTGCAG 1 cut(s) 54
BspPI GGATC 2 cut(s) 137, 150
BspQI GCTCTTC 1 cut(s) 212
BspTI CTTAAG 1 cut(s) 154
BsrBI CCGCTC 1 cut(s) 590
BsrDI GCAATG 2 cut(s) 545, 548
BsrI ACTGG 2 cut(s) 431, 527
BssECI CCNNGG 1 cut(s) 468
BssMI GATC 3 cut(s) 142, 394, 480
Bst4CI ACNGT 1 cut(s) 436
Bst6I CTCTTC 3 cut(s) 177, 212, 561
BstAFI CTTAAG 1 cut(s) 154
BstBAI YACGTR 1 cut(s) 166
BstC8I GCNNGC 2 cut(s) 54, 536
BstDEI CTNAG 1 cut(s) 558
BstF5I GGATG 3 cut(s) 85, 454, 464
BstKTI GATC 3 cut(s) 145, 397, 483
BstMBI GATC 3 cut(s) 142, 394, 480
BstSFI CTRYAG 1 cut(s) 50
BstV1I GCAGC 1 cut(s) 123
BstX2I RGATCY 1 cut(s) 142
BstYI RGATCY 1 cut(s) 142
BsuI GTATCC 1 cut(s) 98
BtsCI GGATG 3 cut(s) 85, 454, 464
Cac8I GCNNGC 2 cut(s) 54, 536
Cfr13I GGNCC 1 cut(s) 505
Csp6I GTAC 1 cut(s) 167
CviAII CATG 1 cut(s) 304
CviJI RGCY 8 cut(s) 21, 56, 94, 153, 159, 222, 364, 534
CviKI_1 RGCY 8 cut(s) 21, 56, 94, 153, 159, 222, 364, 534
CviQI GTAC 1 cut(s) 167
DdeI CTNAG 1 cut(s) 558
DpnI GATC 3 cut(s) 144, 396, 482
DpnII GATC 3 cut(s) 142, 394, 480
Eam1104I CTCTTC 3 cut(s) 177, 212, 561
EarI CTCTTC 3 cut(s) 177, 212, 561
Eco32I GATATC 1 cut(s) 382
Eco47I GGWCC 1 cut(s) 505
Eco57I CTGAAG 1 cut(s) 521
Eco88I CYCGRG 1 cut(s) 591
EcoO109I RGGNCCY 1 cut(s) 505
EcoRI GAATTC 1 cut(s) 34
EcoRV GATATC 1 cut(s) 382
FaeI CATG 1 cut(s) 307
FaiI YATR 6 cut(s) 8, 183, 194, 305, 439, 582
FatI CATG 1 cut(s) 303
FauI CCCGC 2 cut(s) 245, 300
Fnu4HI GCNGC 1 cut(s) 137
FokI GGATG 3 cut(s) 92, 451, 461
Fsp4HI GCNGC 1 cut(s) 137
FspBI CTAG 1 cut(s) 149
GluI GCNGC 1 cut(s) 137
GsaI CCCAGC 1 cut(s) 534
HapII CCGG 1 cut(s) 140
Hin1II CATG 1 cut(s) 307
HincII GTYRAC 2 cut(s) 268, 432
HindII GTYRAC 2 cut(s) 268, 432
HinfI GANTC 3 cut(s) 128, 300, 453
HpaII CCGG 1 cut(s) 140
HphI GGTGA 1 cut(s) 115
Hpy166II GTNNAC 2 cut(s) 268, 432
Hpy188I TCNGA 2 cut(s) 349, 469
Hpy188III TCNNGA 2 cut(s) 176, 250
Hpy8I GTNNAC 2 cut(s) 268, 432
HpyAV CCTTC 2 cut(s) 170, 496
HpyCH4III ACNGT 1 cut(s) 436
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 7 cut(s) 52, 83, 337, 369, 412, 538, 575
HpyF3I CTNAG 1 cut(s) 558
HpySE526I ACGT 1 cut(s) 165
Hsp92II CATG 1 cut(s) 307
Kzo9I GATC 3 cut(s) 142, 394, 480
LguI GCTCTTC 1 cut(s) 212
LmnI GCTCC 3 cut(s) 262, 361, 474
Lsp1109I GCAGC 1 cut(s) 123
LweI GCATC 2 cut(s) 70, 340
MaeI CTAG 1 cut(s) 149
MaeII ACGT 1 cut(s) 165
MalI GATC 3 cut(s) 144, 396, 482
MbiI CCGCTC 1 cut(s) 590
MboI GATC 3 cut(s) 142, 394, 480
MboII GAAGA 6 cut(s) 164, 211, 229, 238, 524, 578
MflI RGATCY 1 cut(s) 142
MluCI AATT 1 cut(s) 34
MmeI TCCRAC 1 cut(s) 11
MnlI CCTC 6 cut(s) 180, 189, 265, 270, 463, 484
MseI TTAA 1 cut(s) 155
MspCI CTTAAG 1 cut(s) 154
MspI CCGG 1 cut(s) 140
NdeII GATC 3 cut(s) 142, 394, 480
NlaIII CATG 1 cut(s) 307
NlaIV GGNNCC 2 cut(s) 144, 528
PciSI GCTCTTC 1 cut(s) 212
PfeI GAWTC 3 cut(s) 128, 300, 453
PkrI GCNGC 1 cut(s) 138
Ppu21I YACGTR 1 cut(s) 166
PpuMI RGGWCCY 1 cut(s) 505
Psp5II RGGWCCY 1 cut(s) 505
PspFI CCCAGC 1 cut(s) 530
PspN4I GGNNCC 2 cut(s) 144, 528
PspPI GGNCC 1 cut(s) 505
PspPPI RGGWCCY 1 cut(s) 505
PstI CTGCAG 1 cut(s) 54
PsuI RGATCY 1 cut(s) 142
RsaI GTAC 1 cut(s) 168
RsaNI GTAC 1 cut(s) 167
SapI GCTCTTC 1 cut(s) 212
SaqAI TTAA 1 cut(s) 155
SatI GCNGC 1 cut(s) 137
Sau3AI GATC 3 cut(s) 142, 394, 480
Sau96I GGNCC 1 cut(s) 505
SetI ASST 5 cut(s) 168, 172, 366, 507, 580
SfaNI GCATC 2 cut(s) 70, 340
SfcI CTRYAG 1 cut(s) 50
SinI GGWCC 1 cut(s) 505
SmlI CTYRAG 1 cut(s) 154
SmoI CTYRAG 1 cut(s) 154
Sse9I AATT 1 cut(s) 34
SsiI CCGC 4 cut(s) 238, 283, 307, 588
SspMI CTAG 1 cut(s) 149
TaaI ACNGT 1 cut(s) 436
TaiI ACGT 1 cut(s) 168
TaqI TCGA 1 cut(s) 319
TasI AATT 1 cut(s) 34
TfiI GAWTC 3 cut(s) 128, 300, 453
Tru1I TTAA 1 cut(s) 155
Tru9I TTAA 1 cut(s) 155
TseI GCWGC 1 cut(s) 136
TspDTI ATGAA 1 cut(s) 453
TspGWI ACGGA 1 cut(s) 461
Vha464I CTTAAG 1 cut(s) 154
VpaK11BI GGWCC 1 cut(s) 505
XapI RAATTY 1 cut(s) 34
XspI CTAG 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.