Rorug02G0313500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000954
Physical Location & Seq
Reverse (-)
37950271 .. 37952755
2485 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0313500.1

Sequence Viewer

Length: 1185 bp
ATGGATGATGGGTGCCACGTAAACAAGGGAGAGAGGGTGGTGTTGGACTTATCGCATACCAAGACTCCAAGGGAGAGAGAAGCCGGAAGCGTGCGTGGAGAGCAAAGTGATAACAAAGTGATGGCTCCTCATGGATTTATCATTTCTATGAAATTTAATAACAAATCTATTCTCCCGAGACGTGATATGTTTTGTCTCTCTATATATATTGCTACCCCGTTGCCTCTTTCCTATCATCCTCTCCATTCTCCGTTCTTCATTCTTCTCAAGCCTCTCGTTCTCTGTGTTCCGCCGTTGAAGCTTCAAGGCTCTTATTACGCCTTCACAAACAAAACCCACTTCTCTACTCTTGCTGATTTAAGCAACTCAAGAATACATCTACCCCAATCCGTTAAGATGGCAACAGAGCAATACTTTGGAGATGCTGTGGTCCGGTCTAACTCCATTGGAGATGATAAATGTTCATTTGGAAAATGGAATCCTGTTTCCGAGGTCTCAGAGTCTGAAAACAAGTCCTGTGGTAACTTCGACTGCAGCATATGCTTAGACTCTGTGCACGATCCGGTGGTGACCCTCTGTGGCCATCTATACTGCTGGCCATGCATATATAAGTGGATTCAGTTCCAGACTCCGTCTTCTGAAAGCCAAGAGAACCAAAAACAGCCACAGTGCCCTGTTTGCAAAACTGAAGTCTCCGAATCCTCTCTAGTCCCACTCTATGGTCGTGGTCAAACAACAAAGCTCACGAAGCCATCTAAAGGCAAGGCTCCCAATCTTGGTATAGTCATTCCAAAAAGACCTCTCGCTCCTTGCGGGGTTGACACACCACGAACCCCAAGTACCAGCCCTTACGGTACTCAGCAACTCCATCACCGGATTAATCCTTATCAATCCCAACAGAACAGCCCTCAAGGAGGTAGTTATGCAGCTGCTTTACCGATGCATGGTCCTGGTGATACAGCAACACAAGTCTTAAATCCGATGGTCGGCTTGTTTGGTGAAATGGTGTATGATAGGGTGTTTGGTAACTCGATAACAAATGTCTACACATACCCTAATTCGTATAATCTTGCTGGCAGCGCTAGTCCTAGGGTGAGAAGACATGTATTGCAGGCTGATAAGTCGCTCAACAGAATGTGTTCTTTTCTGTTTTGTTGCTTTATGTTGTGCCTAATTTTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

394

Amino Acids

43.78

Weight (kDa)

8.75

Isoelectric Point (pI)

48.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 177 - 228 5.9e-06 Ring finger domain
RING_XB3-XBAT31 PF24921 178 - 232 1.7e-09 E3 ubiquitin-protein ligase XB3/XBAT31 RING finger
zf-RING_UBOX PF13445 178 - 225 1.4e-09 RING-type zinc-finger
zf-C3HC4 PF00097 178 - 227 2.7e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 178 - 207 7.1e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 12
AccB7I CCANNNNNTGG 1 cut(s) 719
AccI GTMKAC 1 cut(s) 1044
AciI CCGC 2 cut(s) 290, 813
AclWI GGATC 1 cut(s) 554
AcoI YGGCCR 2 cut(s) 580, 596
AcsI RAATTY 1 cut(s) 152
AcuI CTGAAG 1 cut(s) 708
AfaI GTAC 2 cut(s) 841, 856
AfeI AGCGCT 1 cut(s) 1081
AfiI CCNNNNNNNGG 6 cut(s) 719, 758, 776, 914, 944, 986
AflIII ACRYGT 1 cut(s) 1102
AgsI TTSAA 2 cut(s) 298, 305
AjiI CACGTC 1 cut(s) 182
AjnI CCWGG 1 cut(s) 949
AluBI AGCT 3 cut(s) 301, 742, 929
AluI AGCT 3 cut(s) 301, 742, 929
Alw21I GWGCWC 1 cut(s) 558
Alw26I GTCTC 4 cut(s) 172, 200, 499, 697
Alw44I GTGCAC 1 cut(s) 554
AlwI GGATC 1 cut(s) 554
AlwNI CAGNNNCTG 1 cut(s) 503
Ama87I CYCGRG 1 cut(s) 175
Aor51HI AGCGCT 1 cut(s) 1081
AoxI GGCC 2 cut(s) 580, 596
ApaLI GTGCAC 1 cut(s) 554
ApeKI GCWGC 4 cut(s) 534, 926, 929, 1077
ApoI RAATTY 1 cut(s) 152
ArsI GACNNNNNNTTYG 2 cut(s) 675, 707
AseI ATTAAT 1 cut(s) 879
Asp700I GAANNNNTTC 1 cut(s) 1138
AspA2I CCTAGG 1 cut(s) 1088
AspLEI GCGC 1 cut(s) 1082
AspS9I GGNCC 2 cut(s) 430, 947
AsuHPI GGTGA 5 cut(s) 580, 863, 965, 1010, 1105
AvaI CYCGRG 1 cut(s) 175
AvaII GGWCC 2 cut(s) 430, 947
AvrII CCTAGG 1 cut(s) 1088
BaeGI GKGCMC 2 cut(s) 558, 674
BalI TGGCCA 2 cut(s) 582, 598
BanI GGYRCC 1 cut(s) 12
BbsI GAAGAC 2 cut(s) 627, 1105
Bbv12I GWGCWC 1 cut(s) 558
BbvI GCAGC 4 cut(s) 546, 916, 938, 1089
BccI CCATC 7 cut(s) 2, 115, 391, 591, 760, 876, 976
BceAI ACGGC 1 cut(s) 277
BciT130I CCWGG 1 cut(s) 951
BcoDI GTCTC 4 cut(s) 172, 200, 499, 697
BfaI CTAG 3 cut(s) 707, 1083, 1089
BfmI CTRYAG 1 cut(s) 532
BfoI RGCGCY 1 cut(s) 1083
BisI GCNGC 4 cut(s) 535, 927, 930, 1078
BlnI CCTAGG 1 cut(s) 1088
BlsI GCNGC 4 cut(s) 536, 928, 931, 1079
Bme1390I CCNGG 1 cut(s) 951
Bme18I GGWCC 2 cut(s) 430, 947
BmeT110I CYCGRG 1 cut(s) 175
BmgBI CACGTC 1 cut(s) 182
BmgT120I GGNCC 2 cut(s) 430, 947
BmiI GGNNCC 3 cut(s) 14, 126, 768
BmrFI CCNGG 1 cut(s) 951
BmsI GCATC 2 cut(s) 412, 930
BpiI GAAGAC 2 cut(s) 627, 1105
BpuEI CTTGAG 3 cut(s) 251, 352, 894
BsaAI YACGTR 1 cut(s) 19
BsaI GGTCTC 1 cut(s) 499
BsaJI CCNNGG 3 cut(s) 68, 489, 1088
BsaWI WCCGGW 3 cut(s) 432, 562, 873
BsaXI ACNNNNNCTCC 6 cut(s) 21, 49, 51, 79, 411, 441
Bsc4I CCNNNNNNNGG 6 cut(s) 719, 758, 776, 914, 944, 986
BseBI CCWGG 1 cut(s) 951
BseDI CCNNGG 3 cut(s) 68, 489, 1088
BseGI GGATG 2 cut(s) 10, 235
BseLI CCNNNNNNNGG 6 cut(s) 719, 758, 776, 914, 944, 986
BseMII CTCAG 2 cut(s) 510, 872
BseRI GAGGAG 1 cut(s) 117
BseSI GKGCMC 2 cut(s) 558, 674
BseXI GCAGC 4 cut(s) 546, 916, 938, 1089
BshFI GGCC 2 cut(s) 582, 598
BshNI GGYRCC 1 cut(s) 12
BsiHKAI GWGCWC 1 cut(s) 558
BsiHKCI CYCGRG 1 cut(s) 175
BsiSI CCGG 4 cut(s) 84, 433, 563, 874
BslFI GGGAC 1 cut(s) 695
BslI CCNNNNNNNGG 6 cut(s) 719, 758, 776, 914, 944, 986
BsmAI GTCTC 4 cut(s) 172, 200, 499, 697
BsmBI CGTCTC 1 cut(s) 172
BsmFI GGGAC 1 cut(s) 695
BsnI GGCC 2 cut(s) 582, 598
Bso31I GGTCTC 1 cut(s) 499
BsoBI CYCGRG 1 cut(s) 175
Bsp1286I GDGCHC 2 cut(s) 558, 674
Bsp143I GATC 1 cut(s) 559
BspACI CCGC 2 cut(s) 290, 813
BspANI GGCC 2 cut(s) 582, 598
BspCNI CTCAG 2 cut(s) 509, 871
BspLI GGNNCC 3 cut(s) 14, 126, 768
BspMAI CTGCAG 1 cut(s) 536
BspPI GGATC 1 cut(s) 554
BspT107I GGYRCC 1 cut(s) 12
BspTNI GGTCTC 1 cut(s) 499
BssECI CCNNGG 3 cut(s) 68, 489, 1088
BssMI GATC 1 cut(s) 559
BssT1I CCWWGG 2 cut(s) 68, 1088
Bst2UI CCWGG 1 cut(s) 951
Bst4CI ACNGT 2 cut(s) 669, 854
BstAPI GCANNNNNTGC 1 cut(s) 540
BstBAI YACGTR 1 cut(s) 19
BstC8I GCNNGC 4 cut(s) 92, 596, 1075, 1113
BstDEI CTNAG 3 cut(s) 496, 544, 858
BstEII GGTNACC 1 cut(s) 568
BstENI CCTNNNNNAGG 1 cut(s) 912
BstF5I GGATG 2 cut(s) 10, 235
BstH2I RGCGCY 1 cut(s) 1083
BstHHI GCGC 1 cut(s) 1082
BstKTI GATC 1 cut(s) 562
BstMAI GTCTC 4 cut(s) 172, 200, 499, 697
BstMBI GATC 1 cut(s) 559
BstMWI GCNNNNNNNGC 7 cut(s) 100, 298, 540, 600, 678, 748, 1079
BstNI CCWGG 1 cut(s) 951
BstNSI RCATGY 1 cut(s) 1106
BstPI GGTNACC 1 cut(s) 568
BstSCI CCNGG 1 cut(s) 949
BstSFI CTRYAG 1 cut(s) 532
BstSLI GKGCMC 2 cut(s) 558, 674
BstV1I GCAGC 4 cut(s) 546, 916, 938, 1089
BstV2I GAAGAC 2 cut(s) 627, 1105
BsuRI GGCC 2 cut(s) 582, 598
BtrI CACGTC 1 cut(s) 182
BtsCI GGATG 2 cut(s) 10, 235
BtsIMutI CAGTG 1 cut(s) 674
Cac8I GCNNGC 4 cut(s) 92, 596, 1075, 1113
CaiI CAGNNNCTG 1 cut(s) 503
CfoI GCGC 1 cut(s) 1082
Cfr13I GGNCC 2 cut(s) 430, 947
Csp6I GTAC 2 cut(s) 840, 855
CspCI CAANNNNNGTGG 2 cut(s) 499, 534
CviAII CATG 4 cut(s) 131, 600, 944, 1103
CviQI GTAC 2 cut(s) 840, 855
DdeI CTNAG 3 cut(s) 496, 544, 858
DpnI GATC 1 cut(s) 561
DpnII GATC 1 cut(s) 559
EaeI YGGCCR 2 cut(s) 580, 596
EciI GGCGGA 1 cut(s) 279
Eco130I CCWWGG 2 cut(s) 68, 1088
Eco31I GGTCTC 1 cut(s) 499
Eco47I GGWCC 2 cut(s) 430, 947
Eco47III AGCGCT 1 cut(s) 1081
Eco57I CTGAAG 1 cut(s) 708
Eco88I CYCGRG 1 cut(s) 175
Eco91I GGTNACC 1 cut(s) 568
EcoNI CCTNNNNNAGG 1 cut(s) 912
EcoO65I GGTNACC 1 cut(s) 568
EcoRII CCWGG 1 cut(s) 949
EcoT14I CCWWGG 2 cut(s) 68, 1088
EcoT22I ATGCAT 2 cut(s) 605, 945
ErhI CCWWGG 2 cut(s) 68, 1088
Esp3I CGTCTC 1 cut(s) 172
FaeI CATG 4 cut(s) 134, 603, 947, 1106
FaqI GGGAC 1 cut(s) 695
FatI CATG 4 cut(s) 130, 599, 943, 1102
FauI CCCGC 1 cut(s) 806
FauNDI CATATG 1 cut(s) 539
FblI GTMKAC 1 cut(s) 1044
Fnu4HI GCNGC 4 cut(s) 535, 927, 930, 1078
FokI GGATG 2 cut(s) 17, 222
Fsp4HI GCNGC 4 cut(s) 535, 927, 930, 1078
FspBI CTAG 3 cut(s) 707, 1083, 1089
GlaI GCGC 1 cut(s) 1081
GluI GCNGC 4 cut(s) 535, 927, 930, 1078
HaeII RGCGCY 1 cut(s) 1083
HaeIII GGCC 2 cut(s) 582, 598
HapII CCGG 4 cut(s) 84, 433, 563, 874
HhaI GCGC 1 cut(s) 1082
Hin1II CATG 4 cut(s) 134, 603, 947, 1106
Hin6I GCGC 1 cut(s) 1080
HinP1I GCGC 1 cut(s) 1080
HincII GTYRAC 1 cut(s) 820
HindII GTYRAC 1 cut(s) 820
HindIII AAGCTT 1 cut(s) 299
HinfI GANTC 7 cut(s) 64, 478, 500, 548, 616, 628, 698
HpaII CCGG 4 cut(s) 84, 433, 563, 874
HphI GGTGA 5 cut(s) 580, 863, 965, 1010, 1105
Hpy166II GTNNAC 4 cut(s) 22, 556, 820, 1045
Hpy188I TCNGA 6 cut(s) 490, 499, 505, 640, 697, 981
Hpy188III TCNNGA 4 cut(s) 175, 369, 625, 745
Hpy8I GTNNAC 4 cut(s) 22, 556, 820, 1045
HpyAV CCTTC 1 cut(s) 331
HpyCH4III ACNGT 2 cut(s) 669, 854
HpyCH4IV ACGT 2 cut(s) 18, 181
HpyCH4V TGCA 7 cut(s) 534, 556, 603, 681, 926, 943, 1111
HpyF10VI GCNNNNNNNGC 7 cut(s) 100, 298, 540, 600, 678, 748, 1079
HpyF3I CTNAG 3 cut(s) 496, 544, 858
HpySE526I ACGT 2 cut(s) 18, 181
Hsp92II CATG 4 cut(s) 134, 603, 947, 1106
HspAI GCGC 1 cut(s) 1080
Kzo9I GATC 1 cut(s) 559
LmnI GCTCC 3 cut(s) 130, 772, 811
Lsp1109I GCAGC 4 cut(s) 546, 916, 938, 1089
LweI GCATC 2 cut(s) 412, 930
MaeI CTAG 3 cut(s) 707, 1083, 1089
MaeII ACGT 2 cut(s) 18, 181
MaeIII GTNAC 3 cut(s) 521, 568, 1025
MalI GATC 1 cut(s) 561
MboI GATC 1 cut(s) 559
MboII GAAGA 4 cut(s) 247, 254, 627, 1110
MhlI GDGCHC 2 cut(s) 558, 674
MlsI TGGCCA 2 cut(s) 582, 598
MluCI AATT 3 cut(s) 152, 1057, 1173
MluNI TGGCCA 2 cut(s) 582, 598
MlyI GAGTC 4 cut(s) 58, 509, 542, 622
MmeI TCCRAC 1 cut(s) 24
Mox20I TGGCCA 2 cut(s) 582, 598
Mph1103I ATGCAT 2 cut(s) 605, 945
MroXI GAANNNNTTC 1 cut(s) 1138
MscI TGGCCA 2 cut(s) 582, 598
MseI TTAA 5 cut(s) 156, 359, 393, 879, 974
MslI CAYNNNNRTG 1 cut(s) 146
Msp20I TGGCCA 2 cut(s) 582, 598
MspA1I CMGCKG 1 cut(s) 929
MspI CCGG 4 cut(s) 84, 433, 563, 874
MspR9I CCNGG 1 cut(s) 951
MvaI CCWGG 1 cut(s) 951
MwoI GCNNNNNNNGC 7 cut(s) 100, 298, 540, 600, 678, 748, 1079
NdeI CATATG 1 cut(s) 539
NdeII GATC 1 cut(s) 559
NlaIII CATG 4 cut(s) 134, 603, 947, 1106
NlaIV GGNNCC 3 cut(s) 14, 126, 768
NmuCI GTSAC 1 cut(s) 568
NsiI ATGCAT 2 cut(s) 605, 945
NspI RCATGY 1 cut(s) 1106
PciI ACATGT 1 cut(s) 1102
PdmI GAANNNNTTC 1 cut(s) 1138
PfeI GAWTC 3 cut(s) 478, 616, 698
PflFI GACNNNGTC 1 cut(s) 631
PflMI CCANNNNNTGG 1 cut(s) 719
PkrI GCNGC 4 cut(s) 536, 928, 931, 1079
PleI GAGTC 4 cut(s) 58, 508, 542, 622
PpsI GAGTC 4 cut(s) 58, 508, 542, 622
Ppu21I YACGTR 1 cut(s) 19
PscI ACATGT 1 cut(s) 1102
PshBI ATTAAT 1 cut(s) 879
Psp6I CCWGG 1 cut(s) 949
PspEI GGTNACC 1 cut(s) 568
PspGI CCWGG 1 cut(s) 949
PspN4I GGNNCC 3 cut(s) 14, 126, 768
PspPI GGNCC 2 cut(s) 430, 947
PsrI GAACNNNNNNTAC 2 cut(s) 823, 855
PstI CTGCAG 1 cut(s) 536
PstNI CAGNNNCTG 1 cut(s) 503
PsyI GACNNNGTC 1 cut(s) 631
PvuII CAGCTG 1 cut(s) 929
RsaI GTAC 2 cut(s) 841, 856
RsaNI GTAC 2 cut(s) 840, 855
RseI CAYNNNNRTG 1 cut(s) 146
SaqAI TTAA 5 cut(s) 156, 359, 393, 879, 974
SatI GCNGC 4 cut(s) 535, 927, 930, 1078
Sau3AI GATC 1 cut(s) 559
Sau96I GGNCC 2 cut(s) 430, 947
SchI GAGTC 4 cut(s) 58, 509, 542, 622
ScrFI CCNGG 1 cut(s) 951
SduI GDGCHC 2 cut(s) 558, 674
SetI ASST 8 cut(s) 21, 184, 303, 495, 744, 802, 919, 931
SfaNI GCATC 2 cut(s) 412, 930
SfcI CTRYAG 1 cut(s) 532
SinI GGWCC 2 cut(s) 430, 947
SmiMI CAYNNNNRTG 1 cut(s) 146
SmlI CTYRAG 3 cut(s) 266, 367, 909
SmoI CTYRAG 3 cut(s) 266, 367, 909
Sse9I AATT 3 cut(s) 152, 1057, 1173
SsiI CCGC 2 cut(s) 290, 813
SspMI CTAG 3 cut(s) 707, 1083, 1089
StyD4I CCNGG 1 cut(s) 949
StyI CCWWGG 2 cut(s) 68, 1088
TaaI ACNGT 2 cut(s) 669, 854
TaiI ACGT 2 cut(s) 21, 184
TaqI TCGA 2 cut(s) 528, 1031
TasI AATT 3 cut(s) 152, 1057, 1173
TfiI GAWTC 3 cut(s) 478, 616, 698
Tru1I TTAA 5 cut(s) 156, 359, 393, 879, 974
Tru9I TTAA 5 cut(s) 156, 359, 393, 879, 974
TscAI CASTG 1 cut(s) 674
TseFI GTSAC 1 cut(s) 568
TseI GCWGC 4 cut(s) 534, 926, 929, 1077
Tsp45I GTSAC 1 cut(s) 568
TspDTI ATGAA 3 cut(s) 164, 247, 453
TspGWI ACGGA 3 cut(s) 240, 379, 621
TspRI CASTG 1 cut(s) 674
Tth111I GACNNNGTC 1 cut(s) 631
Van91I CCANNNNNTGG 1 cut(s) 719
VneI GTGCAC 1 cut(s) 554
VpaK11BI GGWCC 2 cut(s) 430, 947
VspI ATTAAT 1 cut(s) 879
XagI CCTNNNNNAGG 1 cut(s) 912
XapI RAATTY 1 cut(s) 152
XceI RCATGY 1 cut(s) 1106
XmaJI CCTAGG 1 cut(s) 1088
XmiI GTMKAC 1 cut(s) 1044
XmnI GAANNNNTTC 1 cut(s) 1138
XspI CTAG 3 cut(s) 707, 1083, 1089
Zsp2I ATGCAT 2 cut(s) 605, 945
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.