FvH4_6g08720

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000954
Physical Location & Seq
Forward (+)
5186714 .. 5189110
2397 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g08720.t2

Sequence Viewer

Length: 678 bp
ATGTTGAGAGTCAGTTTCTTAGTGTTGCTTGCCATGGCTTTGGCAGCAACTAGTGCTCATGCCCGCTTAGACCTATTTTCCCAGCTGCTTACCTCTAACAACCAACTAGAACCTTATCCTGAATGTCAGAAAATTCCATTTCTACTGTGCGACCGTGTCTGCGACCGTTGCGTTTGCGACAGGCGTGCTCCACAGTTTGCAGAGTGCACTTGCGCACAGTGGAAACCATTTGGTGAAGAACTCCAGAAATATTCTGGTGTTACTAACATACGCACGCATGCAGAAAAATTATCTACAGAACTGGAAGCAGTCACCAAACCAGTTTCAAAATCAGTGGGAGGCTTTATCCCATATCCAGAATGCATTGAAAAATGTGACGAGTGTGTAATTTGCACCTTCATATATCCAATTGAAGCTGCACTGTGCTATTGTGGTAAAAAGACTGCTTCCCACTCCAAGAGACCAGCAATCGAATATGCAGAAAAATTACTTGCAAAATTGGACGTAGTCGCCAAACCAATTGCAAAATCAGTGGGAGGCTTTATTCCATACCCCGAATGCATAGAGAAATGTGATGAGTGTGTAATTTGTACACGTCCTTATCCAATCGAGAAAGCATTGTGCTATTGTGGTATGAAGACTGAAGCTGAGAGCAGAGCACTCCCCATCTACATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

226

Amino Acids

25.11

Weight (kDa)

6.79

Isoelectric Point (pI)

61.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 214
AciI CCGC 1 cut(s) 64
AcsI RAATTY 1 cut(s) 132
AcuI CTGAAG 1 cut(s) 663
AfaI GTAC 1 cut(s) 592
AflIII ACRYGT 1 cut(s) 593
AgsI TTSAA 3 cut(s) 327, 368, 413
AhlI ACTAGT 1 cut(s) 50
AjiI CACGTC 1 cut(s) 596
AluBI AGCT 3 cut(s) 85, 416, 647
AluI AGCT 3 cut(s) 85, 416, 647
Alw21I GWGCWC 4 cut(s) 58, 190, 209, 661
Alw26I GTCTC 1 cut(s) 454
Alw44I GTGCAC 1 cut(s) 205
ApaLI GTGCAC 1 cut(s) 205
ApeKI GCWGC 3 cut(s) 44, 85, 416
ApoI RAATTY 1 cut(s) 132
AspLEI GCGC 1 cut(s) 215
AsuHPI GGTGA 2 cut(s) 245, 304
BaeGI GKGCMC 1 cut(s) 209
BbsI GAAGAC 1 cut(s) 644
Bbv12I GWGCWC 4 cut(s) 58, 190, 209, 661
BbvI GCAGC 3 cut(s) 56, 72, 403
BccI CCATC 1 cut(s) 674
BcgI CGANNNNNNTGC 2 cut(s) 167, 201
BcoDI GTCTC 1 cut(s) 454
BcuI ACTAGT 1 cut(s) 50
BfaI CTAG 3 cut(s) 51, 107, 676
BfmI CTRYAG 1 cut(s) 294
BisI GCNGC 3 cut(s) 45, 86, 417
BlsI GCNGC 3 cut(s) 46, 87, 418
BmgBI CACGTC 1 cut(s) 596
BpiI GAAGAC 1 cut(s) 644
BpmI CTGGAG 1 cut(s) 227
BsaI GGTCTC 1 cut(s) 454
BsaJI CCNNGG 1 cut(s) 33
Bse1I ACTGG 2 cut(s) 306, 320
BseDI CCNNGG 1 cut(s) 33
BseMII CTCAG 1 cut(s) 639
BseNI ACTGG 2 cut(s) 306, 320
BseSI GKGCMC 1 cut(s) 209
BseXI GCAGC 3 cut(s) 56, 72, 403
BseYI CCCAGC 1 cut(s) 81
BsgI GTGCAG 1 cut(s) 402
Bsh1285I CGRYCG 2 cut(s) 154, 166
BsiEI CGRYCG 2 cut(s) 154, 166
BsiHKAI GWGCWC 4 cut(s) 58, 190, 209, 661
BsmAI GTCTC 1 cut(s) 454
BsmI GAATGC 2 cut(s) 365, 563
Bso31I GGTCTC 1 cut(s) 454
Bsp1286I GDGCHC 4 cut(s) 58, 190, 209, 661
Bsp1407I TGTACA 1 cut(s) 590
Bsp19I CCATGG 1 cut(s) 33
BspACI CCGC 1 cut(s) 64
BspCNI CTCAG 1 cut(s) 640
BspTNI GGTCTC 1 cut(s) 454
BsrGI TGTACA 1 cut(s) 590
BsrI ACTGG 2 cut(s) 306, 320
BssECI CCNNGG 1 cut(s) 33
BssT1I CCWWGG 1 cut(s) 33
Bst4CI ACNGT 6 cut(s) 147, 155, 167, 195, 219, 423
BstAPI GCANNNNNTGC 1 cut(s) 53
BstAUI TGTACA 1 cut(s) 590
BstC8I GCNNGC 5 cut(s) 30, 64, 186, 275, 279
BstDEI CTNAG 3 cut(s) 19, 67, 648
BstDSI CCRYGG 1 cut(s) 33
BstHHI GCGC 1 cut(s) 215
BstMAI GTCTC 1 cut(s) 454
BstMCI CGRYCG 2 cut(s) 154, 166
BstMWI GCNNNNNNNGC 3 cut(s) 44, 53, 168
BstNSI RCATGY 1 cut(s) 281
BstSFI CTRYAG 1 cut(s) 294
BstSLI GKGCMC 1 cut(s) 209
BstV1I GCAGC 3 cut(s) 56, 72, 403
BstV2I GAAGAC 1 cut(s) 644
BstXI CCANNNNNNTGG 1 cut(s) 40
BtgI CCRYGG 1 cut(s) 33
BtrI CACGTC 1 cut(s) 596
BtsIMutI CAGTG 4 cut(s) 224, 339, 419, 537
Cac8I GCNNGC 5 cut(s) 30, 64, 186, 275, 279
CfoI GCGC 1 cut(s) 215
Csp6I GTAC 1 cut(s) 591
CspCI CAANNNNNGTGG 4 cut(s) 315, 350, 513, 548
CviAII CATG 3 cut(s) 34, 59, 278
CviJI RGCY 6 cut(s) 38, 85, 342, 416, 540, 647
CviKI_1 RGCY 6 cut(s) 38, 85, 342, 416, 540, 647
CviQI GTAC 1 cut(s) 591
DdeI CTNAG 3 cut(s) 19, 67, 648
Eco130I CCWWGG 1 cut(s) 33
Eco31I GGTCTC 1 cut(s) 454
Eco57I CTGAAG 1 cut(s) 663
EcoT14I CCWWGG 1 cut(s) 33
EcoT22I ATGCAT 2 cut(s) 365, 563
ErhI CCWWGG 1 cut(s) 33
FaeI CATG 3 cut(s) 37, 62, 281
FatI CATG 3 cut(s) 33, 58, 277
FauI CCCGC 1 cut(s) 71
Fnu4HI GCNGC 3 cut(s) 45, 86, 417
Fsp4HI GCNGC 3 cut(s) 45, 86, 417
FspBI CTAG 3 cut(s) 51, 107, 676
FspI TGCGCA 1 cut(s) 214
GlaI GCGC 1 cut(s) 214
GluI GCNGC 3 cut(s) 45, 86, 417
GsaI CCCAGC 1 cut(s) 85
GsuI CTGGAG 1 cut(s) 227
HhaI GCGC 1 cut(s) 215
Hin1II CATG 3 cut(s) 37, 62, 281
Hin6I GCGC 1 cut(s) 213
HinP1I GCGC 1 cut(s) 213
HinfI GANTC 1 cut(s) 9
HphI GGTGA 2 cut(s) 245, 304
Hpy166II GTNNAC 2 cut(s) 207, 593
Hpy188I TCNGA 1 cut(s) 129
Hpy188III TCNNGA 4 cut(s) 119, 244, 356, 610
Hpy8I GTNNAC 2 cut(s) 207, 593
HpyAV CCTTC 1 cut(s) 406
HpyCH4III ACNGT 6 cut(s) 147, 155, 167, 195, 219, 423
HpyCH4IV ACGT 2 cut(s) 504, 595
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 53, 168
HpyF3I CTNAG 3 cut(s) 19, 67, 648
HpySE526I ACGT 2 cut(s) 504, 595
Hsp92II CATG 3 cut(s) 37, 62, 281
HspAI GCGC 1 cut(s) 213
LmnI GCTCC 1 cut(s) 193
LpnPI CCDG 9 cut(s) 95, 132, 166, 240, 257, 287, 333, 369, 477
Lsp1109I GCAGC 3 cut(s) 56, 72, 403
MaeI CTAG 3 cut(s) 51, 107, 676
MaeII ACGT 2 cut(s) 504, 595
MaeIII GTNAC 3 cut(s) 259, 310, 374
MboII GAAGA 2 cut(s) 248, 649
MfeI CAATTG 2 cut(s) 408, 519
MhlI GDGCHC 4 cut(s) 58, 190, 209, 661
MluCI AATT 8 cut(s) 132, 287, 387, 408, 485, 497, 519, 585
MlyI GAGTC 1 cut(s) 18
MnlI CCTC 3 cut(s) 103, 332, 530
Mph1103I ATGCAT 2 cut(s) 365, 563
MspA1I CMGCKG 1 cut(s) 85
MunI CAATTG 2 cut(s) 408, 519
Mva1269I GAATGC 2 cut(s) 365, 563
MwoI GCNNNNNNNGC 3 cut(s) 44, 53, 168
NcoI CCATGG 1 cut(s) 33
NlaIII CATG 3 cut(s) 37, 62, 281
NmuCI GTSAC 2 cut(s) 310, 374
NsbI TGCGCA 1 cut(s) 214
NsiI ATGCAT 2 cut(s) 365, 563
NspI RCATGY 1 cut(s) 281
PaeI GCATGC 1 cut(s) 281
PctI GAATGC 2 cut(s) 365, 563
PflFI GACNNNGTC 2 cut(s) 155, 506
PkrI GCNGC 3 cut(s) 46, 87, 418
PleI GAGTC 1 cut(s) 17
PpsI GAGTC 1 cut(s) 17
PspFI CCCAGC 1 cut(s) 81
PsyI GACNNNGTC 2 cut(s) 155, 506
PvuII CAGCTG 1 cut(s) 85
RsaI GTAC 1 cut(s) 592
RsaNI GTAC 1 cut(s) 591
SatI GCNGC 3 cut(s) 45, 86, 417
SchI GAGTC 1 cut(s) 18
SduI GDGCHC 4 cut(s) 58, 190, 209, 661
SetI ASST 9 cut(s) 75, 87, 95, 115, 398, 418, 507, 598, 649
SfcI CTRYAG 1 cut(s) 294
SpeI ACTAGT 1 cut(s) 50
SphI GCATGC 1 cut(s) 281
Sse9I AATT 8 cut(s) 132, 287, 387, 408, 485, 497, 519, 585
SsiI CCGC 1 cut(s) 64
SspI AATATT 1 cut(s) 251
SspMI CTAG 3 cut(s) 51, 107, 676
StyI CCWWGG 1 cut(s) 33
TaaI ACNGT 6 cut(s) 147, 155, 167, 195, 219, 423
TaiI ACGT 2 cut(s) 507, 598
TaqI TCGA 2 cut(s) 471, 609
TasI AATT 8 cut(s) 132, 287, 387, 408, 485, 497, 519, 585
TatI WGTACW 1 cut(s) 590
TscAI CASTG 4 cut(s) 224, 339, 426, 537
TseFI GTSAC 2 cut(s) 310, 374
TseI GCWGC 3 cut(s) 44, 85, 416
Tsp45I GTSAC 2 cut(s) 310, 374
TspDTI ATGAA 2 cut(s) 388, 650
TspRI CASTG 4 cut(s) 224, 339, 426, 537
Tth111I GACNNNGTC 2 cut(s) 155, 506
VneI GTGCAC 1 cut(s) 205
XapI RAATTY 1 cut(s) 132
XceI RCATGY 1 cut(s) 281
XcmI CCANNNNNNNNNTGG 1 cut(s) 251
XspI CTAG 3 cut(s) 51, 107, 676
Zsp2I ATGCAT 2 cut(s) 365, 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.