RchiOBHm_Chr3g0458851

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000954
Physical Location & Seq
Forward (+)
7438882 .. 7440371
1490 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42547

Sequence Viewer

Length: 441 bp
ATGTTGAGAGTAAGTTTCTTAATGTTGCTTGCCATGGCTTTGGCAGCAACTAGTGCTCATGCTCGCCTAGATCTATTTGGCCAGCTACTCACCTCCAAGGACCAACCAAATCCCAATTATGAATGCCAGAAAATTCCATTTCTACTATGCGGCCGTGTCTGTGACGCTTGCATTTGCGATAGACGTGCTCCAGAGTTTGCAGAGTGCACTTGCGGACAGTGGAAACCTTATGCAGAACTCCAAAAATCTGACGAAGCACAAGTGCAGGTGATAGGATTAGAAAAATCTTCTATTAAAGCTAAGTCTTCAGGCGTCATTCCATATAATGAATGCATCAAAGTCTGCAAAATTTGTGCTTGTACACTAATCTGGCCCCCTGAGGCAAATTGGTGCATTGATTGCCGCGAGACTGAAGCTGAAGGCAGAACCCTCCCCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

146

Amino Acids

16.25

Weight (kDa)

5.86

Isoelectric Point (pI)

47.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 256
Acc36I ACCTGC 1 cut(s) 256
AccII CGCG 1 cut(s) 405
AciI CCGC 3 cut(s) 150, 213, 403
AcoI YGGCCR 2 cut(s) 79, 151
AcsI RAATTY 2 cut(s) 132, 348
AcuI CTGAAG 3 cut(s) 291, 432, 438
AcyI GRCGYC 1 cut(s) 312
AfaI GTAC 1 cut(s) 361
AhlI ACTAGT 1 cut(s) 50
AjiI CACGTC 1 cut(s) 185
AluBI AGCT 3 cut(s) 85, 299, 416
AluI AGCT 3 cut(s) 85, 299, 416
Alw21I GWGCWC 3 cut(s) 58, 190, 209
Alw26I GTCTC 1 cut(s) 401
Alw44I GTGCAC 1 cut(s) 205
AoxI GGCC 3 cut(s) 79, 151, 371
ApaLI GTGCAC 1 cut(s) 205
ApeKI GCWGC 1 cut(s) 44
ApoI RAATTY 2 cut(s) 132, 348
AspS9I GGNCC 2 cut(s) 100, 372
AsuHPI GGTGA 2 cut(s) 82, 280
AvaII GGWCC 1 cut(s) 100
AxyI CCTNAGG 1 cut(s) 378
BaeGI GKGCMC 1 cut(s) 209
BalI TGGCCA 1 cut(s) 81
BbsI GAAGAC 1 cut(s) 297
Bbv12I GWGCWC 3 cut(s) 58, 190, 209
BbvI GCAGC 1 cut(s) 56
BceAI ACGGC 1 cut(s) 138
BcgI CGANNNNNNTGC 2 cut(s) 167, 201
BcoDI GTCTC 1 cut(s) 401
BcuI ACTAGT 1 cut(s) 50
BfaI CTAG 3 cut(s) 51, 68, 439
BfuAI ACCTGC 1 cut(s) 256
BglII AGATCT 1 cut(s) 70
BisI GCNGC 3 cut(s) 45, 151, 403
BlsI GCNGC 3 cut(s) 46, 152, 404
Bme18I GGWCC 1 cut(s) 100
BmgBI CACGTC 1 cut(s) 185
BmgT120I GGNCC 2 cut(s) 100, 372
BmiI GGNNCC 1 cut(s) 374
BmsI GCATC 1 cut(s) 342
BpiI GAAGAC 1 cut(s) 297
BpmI CTGGAG 1 cut(s) 174
BsaHI GRCGYC 1 cut(s) 312
BsaJI CCNNGG 2 cut(s) 33, 96
Bse21I CCTNAGG 1 cut(s) 378
BseDI CCNNGG 2 cut(s) 33, 96
BseMII CTCAG 1 cut(s) 369
BseSI GKGCMC 1 cut(s) 209
BseX3I CGGCCG 1 cut(s) 151
BseXI GCAGC 1 cut(s) 56
BsgI GTGCAG 1 cut(s) 284
Bsh1236I CGCG 1 cut(s) 405
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 3 cut(s) 81, 153, 373
BsiEI CGRYCG 1 cut(s) 154
BsiHKAI GWGCWC 3 cut(s) 58, 190, 209
BsmAI GTCTC 1 cut(s) 401
BsmI GAATGC 2 cut(s) 128, 335
BsnI GGCC 3 cut(s) 81, 153, 373
Bsp1286I GDGCHC 3 cut(s) 58, 190, 209
Bsp1407I TGTACA 1 cut(s) 359
Bsp143I GATC 1 cut(s) 70
Bsp19I CCATGG 1 cut(s) 33
BspACI CCGC 3 cut(s) 150, 213, 403
BspANI GGCC 3 cut(s) 81, 153, 373
BspCNI CTCAG 1 cut(s) 370
BspFNI CGCG 1 cut(s) 405
BspLI GGNNCC 1 cut(s) 374
BspMI ACCTGC 1 cut(s) 256
BsrGI TGTACA 1 cut(s) 359
BssECI CCNNGG 2 cut(s) 33, 96
BssMI GATC 1 cut(s) 70
BssNI GRCGYC 1 cut(s) 312
BssT1I CCWWGG 2 cut(s) 33, 96
Bst4CI ACNGT 1 cut(s) 219
BstACI GRCGYC 1 cut(s) 312
BstAPI GCANNNNNTGC 2 cut(s) 53, 399
BstAUI TGTACA 1 cut(s) 359
BstC8I GCNNGC 4 cut(s) 30, 64, 83, 169
BstDEI CTNAG 2 cut(s) 300, 378
BstDSI CCRYGG 1 cut(s) 33
BstFNI CGCG 1 cut(s) 405
BstKTI GATC 1 cut(s) 73
BstMAI GTCTC 1 cut(s) 401
BstMBI GATC 1 cut(s) 70
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 3 cut(s) 44, 53, 399
BstSLI GKGCMC 1 cut(s) 209
BstUI CGCG 1 cut(s) 405
BstV1I GCAGC 1 cut(s) 56
BstV2I GAAGAC 1 cut(s) 297
BstX2I RGATCY 1 cut(s) 70
BstXI CCANNNNNNTGG 1 cut(s) 40
BstYI RGATCY 1 cut(s) 70
BstZI CGGCCG 1 cut(s) 151
Bsu36I CCTNAGG 1 cut(s) 378
BsuRI GGCC 3 cut(s) 81, 153, 373
BtgI CCRYGG 1 cut(s) 33
BtrI CACGTC 1 cut(s) 185
BtsIMutI CAGTG 1 cut(s) 224
BveI ACCTGC 1 cut(s) 256
Cac8I GCNNGC 4 cut(s) 30, 64, 83, 169
Cfr13I GGNCC 2 cut(s) 100, 372
CseI GACGC 2 cut(s) 173, 301
Csp6I GTAC 1 cut(s) 360
CviAII CATG 2 cut(s) 34, 59
CviJI RGCY 7 cut(s) 38, 81, 85, 153, 299, 373, 416
CviKI_1 RGCY 7 cut(s) 38, 81, 85, 153, 299, 373, 416
CviQI GTAC 1 cut(s) 360
DdeI CTNAG 2 cut(s) 300, 378
DpnI GATC 1 cut(s) 72
DpnII GATC 1 cut(s) 70
EaeI YGGCCR 2 cut(s) 79, 151
EagI CGGCCG 1 cut(s) 151
EclXI CGGCCG 1 cut(s) 151
Eco130I CCWWGG 2 cut(s) 33, 96
Eco47I GGWCC 1 cut(s) 100
Eco52I CGGCCG 1 cut(s) 151
Eco57I CTGAAG 3 cut(s) 291, 432, 438
Eco81I CCTNAGG 1 cut(s) 378
EcoT14I CCWWGG 2 cut(s) 33, 96
EcoT22I ATGCAT 1 cut(s) 335
ErhI CCWWGG 2 cut(s) 33, 96
FaeI CATG 2 cut(s) 37, 62
FaiI YATR 7 cut(s) 35, 60, 120, 148, 231, 322, 324
FatI CATG 2 cut(s) 33, 58
Fnu4HI GCNGC 3 cut(s) 45, 151, 403
Fsp4HI GCNGC 3 cut(s) 45, 151, 403
FspBI CTAG 3 cut(s) 51, 68, 439
GluI GCNGC 3 cut(s) 45, 151, 403
GsuI CTGGAG 1 cut(s) 174
HaeIII GGCC 3 cut(s) 81, 153, 373
HgaI GACGC 2 cut(s) 173, 301
Hin1I GRCGYC 1 cut(s) 312
Hin1II CATG 2 cut(s) 37, 62
HphI GGTGA 2 cut(s) 82, 280
Hpy166II GTNNAC 2 cut(s) 207, 362
Hpy188I TCNGA 1 cut(s) 250
Hpy188III TCNNGA 1 cut(s) 191
Hpy8I GTNNAC 2 cut(s) 207, 362
HpyAV CCTTC 1 cut(s) 413
HpyCH4III ACNGT 1 cut(s) 219
HpyCH4IV ACGT 1 cut(s) 184
HpyCH4V TGCA 8 cut(s) 171, 200, 207, 233, 265, 333, 345, 393
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 53, 399
HpyF3I CTNAG 2 cut(s) 300, 378
HpySE526I ACGT 1 cut(s) 184
Hsp92I GRCGYC 1 cut(s) 312
Hsp92II CATG 2 cut(s) 37, 62
Kzo9I GATC 1 cut(s) 70
LmnI GCTCC 1 cut(s) 193
LpnPI CCDG 7 cut(s) 95, 140, 204, 251, 294, 355, 390
Lsp1109I GCAGC 1 cut(s) 56
LweI GCATC 1 cut(s) 342
MaeI CTAG 3 cut(s) 51, 68, 439
MaeII ACGT 1 cut(s) 184
MaeIII GTNAC 1 cut(s) 161
MalI GATC 1 cut(s) 72
MboI GATC 1 cut(s) 70
MboII GAAGA 2 cut(s) 279, 297
MflI RGATCY 1 cut(s) 70
MhlI GDGCHC 3 cut(s) 58, 190, 209
MlsI TGGCCA 1 cut(s) 81
MluCI AATT 4 cut(s) 115, 132, 348, 385
MluNI TGGCCA 1 cut(s) 81
MnlI CCTC 3 cut(s) 103, 373, 440
Mox20I TGGCCA 1 cut(s) 81
Mph1103I ATGCAT 1 cut(s) 335
MscI TGGCCA 1 cut(s) 81
MseI TTAA 2 cut(s) 20, 294
Msp20I TGGCCA 1 cut(s) 81
Mva1269I GAATGC 2 cut(s) 128, 335
MvnI CGCG 1 cut(s) 405
MwoI GCNNNNNNNGC 3 cut(s) 44, 53, 399
NcoI CCATGG 1 cut(s) 33
NdeII GATC 1 cut(s) 70
NlaIII CATG 2 cut(s) 37, 62
NlaIV GGNNCC 1 cut(s) 374
NmuCI GTSAC 1 cut(s) 161
NsiI ATGCAT 1 cut(s) 335
PaqCI CACCTGC 1 cut(s) 256
PctI GAATGC 2 cut(s) 128, 335
PkrI GCNGC 3 cut(s) 46, 152, 404
PspN4I GGNNCC 1 cut(s) 374
PspPI GGNCC 2 cut(s) 100, 372
PsuI RGATCY 1 cut(s) 70
RsaI GTAC 1 cut(s) 361
RsaNI GTAC 1 cut(s) 360
SaqAI TTAA 2 cut(s) 20, 294
SatI GCNGC 3 cut(s) 45, 151, 403
Sau3AI GATC 1 cut(s) 70
Sau96I GGNCC 2 cut(s) 100, 372
SduI GDGCHC 3 cut(s) 58, 190, 209
SetI ASST 7 cut(s) 87, 95, 187, 229, 270, 301, 418
SfaNI GCATC 1 cut(s) 342
SinI GGWCC 1 cut(s) 100
SpeI ACTAGT 1 cut(s) 50
Sse9I AATT 4 cut(s) 115, 132, 348, 385
SsiI CCGC 3 cut(s) 150, 213, 403
SspMI CTAG 3 cut(s) 51, 68, 439
StyI CCWWGG 2 cut(s) 33, 96
TaaI ACNGT 1 cut(s) 219
TaiI ACGT 1 cut(s) 187
TasI AATT 4 cut(s) 115, 132, 348, 385
TatI WGTACW 1 cut(s) 359
TauI GCSGC 2 cut(s) 153, 405
Tru1I TTAA 2 cut(s) 20, 294
Tru9I TTAA 2 cut(s) 20, 294
TscAI CASTG 1 cut(s) 224
TseFI GTSAC 1 cut(s) 161
TseI GCWGC 1 cut(s) 44
Tsp45I GTSAC 1 cut(s) 161
TspDTI ATGAA 2 cut(s) 135, 342
TspRI CASTG 1 cut(s) 224
VneI GTGCAC 1 cut(s) 205
VpaK11BI GGWCC 1 cut(s) 100
XapI RAATTY 2 cut(s) 132, 348
XspI CTAG 3 cut(s) 51, 68, 439
Zsp2I ATGCAT 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.