MD12G1104900.v1.1

Protein SHOOT GRAVITROPISM

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004148
Physical Location & Seq
Reverse (-)
16517264 .. 16539092
21829 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1104900.v1.1.491

Sequence Viewer

Length: 5196 bp
ATGGCGTCTTCGAGCTCTGGAAGTTCAATTCCCGCTCCAGAGGCTGTTCAAGTTTTGGTTTCGTTACTTGCTGATGAATCTCCCATCGTCAGGGAGGCCTCCATTGCCTCCCTCAAGGACGTTGCTACCCTAAACCCTCTTCTAGTGCTTGATTGTTGCTCGGCAATATCGCGAGGAGGGCGTCGGCGTTTTGGTAACATGGCTGGGGTTTTCCAAGTAATGGCATATGGTGTTCGAGCCTTGGACAAGGATGATGTCGACCCTCCTTTCATGGCTAAGCTTGCTAAGATTGCAACGGCCGAAATCATATCCTCGAAGGAGCTCAATGCGGACTGGCAAAGAGCTGCGTCAGCCGTTCTTGTTTCAATCGGCAGACATTTGCCAGACCTAATGATGGAAGAAATTTTTCTTCATTTGCCTGGGCCAAACTCAGCTTTACCAGCTATGGTTCAGATACTAGCAGACTTTGCTCATGCTGATGCATTGCAGTTCACCCCACGATTGAAAGGTGTGCTTTCACGGGTTCTACCTATTCTTGGAAATGTACGAGATGCCCATCGGCCTATTTTTGCTAATGCTATTAAGTGCTGGTGTCAGGCTGTTTGGCAGAATAGCCTGGATATCCCTTCGCATTCACCTTTGGATGGTGACATCATGTCATTTCTGAATTCTGTTTTTGAGCTTTTATTGAAAGTTTGGGCTGCTTCACGAGATCTAAAGGTTCGCATGTCATCTGTAGATGCATTAGGACAGATGGTTGGCCTTATTACCCGGGCACAATTAAAGGCGGCTTTACCCAGACTTGTCCCCACGATATTGGAATTGTATAAAAGAGATCAAGATATTACCTTTTTGGCAACATGCAGTCTTCACAATCTCTTAAATGCTTCTATACTGTCAGAAAGTGGTCCTCCTTTACTTGATTTTGAGTTTGAGCTTACAATTATTTTATCAACACTCCTGCCAGTGGTTTGCATCAATAATGACAACAAAGAACACTCAGATTTCTCAGTTGGACTGAAGACTTACAACGAAGTTCAGCGTTGCTTCCTGACAATTGGCTTGGTATACCCTGAGGATTTATTTGTCTTCCTTATAAATAAATGCAGATTGAAGGAAGAACCCTTGACGTTTGGTGCACTATGTGTTCTAAAACATCTCTTACCAAGGTTGTCTGAAGCTTGGCACAGTAAACGGCCTTTTCTTGTTGAAGCTGTACAGTTCTTGATAGATGAGCAAAATTTAGGTGTCCAAAAGGCACTTTCAGAGTTGATAGTCGTTATGGCTTCTCATTGTTACTTAATTGGTCCATCCGGGGAGCTGTTTGTAGAATATCTTGTGCGTCACTGTGCTTTAACAGATAAGGATAGAAGTGATTTTGAGAGGTCAAAGGATGCATCAGGCAACCCCTATATACCTTTCCAATACAAGAGATCAGAGGTGAAGATTGGGACAATTTGTCCGACAGAGTTGAGGGCAATCTGTGAAAAGGGCCTTCTTCTGCTGACTATTACGATCCCTGAAATGGAGCATATACTTTGGCCTTTTCTGTTAAAGATGATTATTCCCCAGGCTTATACTGGTGCAGTTGCTATGGTTTGCAGATGCATCTCAGAATTGTGCAGGAATAGAGCATCTAATAGTGATATCATGCTCTCTGAATGCAAAGCTCGTGCTGATTTGCCAAATCCAGAGCTTTTTGTTCGTTTGGTGGTGCTTTTGCATGATCCTTTAGCAAGGGAGCAGCGGGCGAGTCAGATTTTGACAGTTCTTTGTCATCTGGCGCCTCTCTTTCCAAAAAATATCACCTTGTTTTGGCAAGATGAGATTCCAAAATTAAAGGCATATGTTAGTGACACAGAAGATTTAAAGCAAGATCCTTCTTATCAAGAAACTTGGGACGACATGATAATCAATTTTTTTGCAGAATCTTTGGATGTGATTCAAGATGCTGACTGGATGAGATCCCTTGGGAATGCTATTACCCAACAATATGAACTTTATACATCTGATGATGAACACTCAGCACTTCTTCATCGCTGCTTTGGCGTGTTTCTTCAAAAAGTTAATGACAGGGCTTATGTCCGTCATAAGATAGATTGGATGTATAAACAAGCTAACATCACAAATCCAACCAATAGGCTTGGTCTGGCAAAAGCCATGGGATTGGTTGCAGCATCCCACTTGGACACTGTCCTGGAAAAGCTGAAAGGCATTCTGGATAATGTTGGGGAAAGTATCTTCCGAAGATTCCTATCTTTTTTCTCTGATGATTTCAAAACAGAAGAATCTGATGATGTACATGCTGCTTTGGCTCTAATGTATGGATATGCTGCAAAATATGCTCCATCAACAGTTATTGAAGCCAGAATAGACGCACTTGTTTTTGTCTTTGGGACTAATATGCTCTCGCGGCTTCTTCATGTGCGACATCCCACTGCAAAGCAGGCAGTTATTACTGCTATTGATTTACTAGGTCGCGCTGTCATCAATGCTGCAGAAAATGGCTCGTCCTTTCCATTAAAAAGAAGGGACCAAATGCTTGACTATATATTAACCTTGATGGGCCGAGATGATAGTGAAAGCTTCTCCGATTCTACTCTTGAATTTCTGCACACACAAGCTCGTGCTTTAAGTGCCTGTACTACGTTGGTCTCCGTAGAGCCAAAACTAACAATTGAAACCAGAAACCATGTATTAAAGGCCACCTTAGGTTTCTTTGCTTTACCAAATGATCCAGTGGATGTTATCAATCCTCTTATCAACAACCTTATCACTCTCTTGTGTGCAATTCTTCTTACAAGTGGAGAAGATGGGAGAAGTCGAGCAGAGCAGTTATCCCATATCTTGAGACAAATTGATCAGTATGTTTCTTCACCTGTGGATTGTCAAAGACGAAGAGGTTGTCTTGCAGTACATGAGATACTTCTCAAGTTTCGGACAGTTTGCACCACTGCAAATTGTGCCCTGGGCTGCCAAGGAAGTTGCACGCACAGCAAGCAAATTGACCGTAATTTGAATAGGAACTTTTCCAACTTACCATCTGCATTTGTATTGCCAAGTCGTGAGGCATTGTCATTGGGAGACAGGGTCATTATGTATCTTCCACGTTGTGCAGACACTAACTCGGAAGTTAGAAAAGCCTCCGCTCAGATTCTTGATCAACTTTTTAGCATTGCGCTTTCACTTCCACGGCCTTCAACTACTAGCTACGGCATGGACATAGAAATATCGTACAGGGCTTTATCATCGCTTGAGGATGTCATAGCTATTTTGAGGAGTGATGCTTCTATAGATCCATCTGAGGTTTTCAACAGAATTATTTCCTCTGTCTGTGTATTACTAACAAAGAGTGAGCTAGTAGCCACCCTGCATGGTTGCACAGCAGCTGTATGCGACAAGATCAAGCAATCAGCGGAGGGGGCCATTCAAGCTGTAATTGAGTTTGTCACAAGAAGGGGAACTGAGCTAAGTGAAACTGATGTATCAAGGACAACTCAAGCTTTGCTGATGGCTACGGCCCATGTGACTGAGAAGCATTTACGCCAGGAAACTCTTGCTGCTATTTCTTCTCTAGCTGAGAGCACTAGTTCAAAAGTTGTCTTCAACGAAGTTTTAGCTACATCTGGAAGGGACATAGTAACAAAAGATGTATCTAGGCTACGCGGTGGCTGGCCAATGCAGGATGCATTTTATGCATTTTCCCAGCATACAGTTCTTTCATCTTTGTTCCTGGAGCATGTCATATCTGTCCTTGACCAGTTTCCCATCCTTAAAGGTGATTCAGAAAAAGGAGAGAATCCTAGCCATTTAGTTGACGGTCAATTGGAGGATGACATTCTGCAAGCTGCTATTATAGCTGTAACTGCATTTTTCAGAGGTGGTGGTAAAATAGGAAAAAAAGCTGTGCAACAAAACTATGCTTCTGTTCTTGCTGAGCTTACTCTCCAATTGGGAAGTTGTCATGGTCTAGCTAGCCGTGGTCAACATGATCCATTACGGGCCCTTTTAACTGCGTTCCAAGCGTTCTGTGAATGTGTTGGAGATCTTGAGATGGGAAAGATTTTGGCCAGAGATGGGGAGCACAATGAAAATGAGAGGTGGATCAATCTTATTGGAGAGATAGCAGGTTGCGTCTCGATAAAAAGACCAAAAGAGGTTCAAAGCATATGTGTAATTTTAAGTAAATCATTAAATCGACACCAAAGATACCAAAGGGAAGCTGCAGCTGCTGCACTGTCAGAGTTTGTCCGCTACAGTGATGGGTTTGGATCCCTATTGGAGCAGATTGTGGAGGTATTGTGTCGACATGTTTCAGATGAATCTCCAACAGTTAGGCGCTTGTGTTTGAGAGGACTAGTACAGATACCATCTATGCAGATGTTGCAGTACACAGCTCAAGTTCTGGGTGTAATATTAGCTTTGCTAGATGATTCCGATGAATCTGTGCAATTAACTGCAGTTTCTTGCTTGCTCACGATGCTTGAGTCGTCACCCAAGGATGCTGTGGATCCCATTTTGCTTAGTCTTTCTCTGCGGCTTCGAAATCTTCTGATATGTACAAATACGAAGATGCGAGCTAATGCTTTTGCAGCTTTTGGAGCACTAAGTAACTATGGAATTGGTCCGCAACATGAGGCATTTCTCGAGCAGGTACATACCGTAATCCCACGCTTGGTTCTGCATTTGCATGATGATGACGTTAGTGTGAGACAGGCTTGCCGGACTACTCTGAAACAAATTGCCCCATTACTGGAAATGGATGGACTCCTCCCCTTGTTCAATATGCATTCTTTCAATCATGATCATCGAACTGACTACGAGGGTTTTGTCAGAGACCTCACAAAGCAATTTGTTCAGCATCATCCCTCAAGACTTGATACTTACATGGCATCTACAATACAGGCTTTCGATGCACCATGGCCGATAATTCAGGCTAATGCTATATACTTCTCCAGCTGTATGCTATCTCTGTCCGATGATCAGCACATCTTGAATCAATATTATGCGCAGGTAGTATTCGGAACGCTAGTAGGAAAAATGAGTAAGTCAGCGGATGCAGTAGTGAGAGCTACGTGCTCTTCTGCTCTAGGTTTATTATTAAAATTCTCAAAGTCATCCTCATGGAAATCAGCTCGAGTTGATCGTACGGACTCAAGTCACTCAATACGAAAGGGCCATGATTTTTCAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cnd1

Protein Analysis

1732

Amino Acids

191.94

Weight (kDa)

6.07

Isoelectric Point (pI)

41.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEAT_MROH2B_1st PF23221 46 - 253 3.9e-27 MROH2B-like, HEAT repeats
HEAT_Maestro_2 PF23210 258 - 905 4.3e-95 MROH2B-like, HEAT repeats
HEAT_Maestro PF21047 1023 - 1202 5.3e-10 Maestro, HEAT repeats
TOR1L1_N PF24714 1412 - 1582 2.5e-07 TORTIFOLIA1 N-terminal
Cnd1 PF12717 1423 - 1503 6.5e-06 non-SMC mitotic condensation complex subunit 1
HEAT_MROH2B_C PF23227 1430 - 1700 2.5e-45 MROH2B-like, HEAT repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1097
Acc16I TGCGCA 1 cut(s) 5010
Acc36I ACCTGC 3 cut(s) 4118, 4642, 5002
AccB1I GGYRCC 1 cut(s) 1783
AccB7I CCANNNNNTGG 1 cut(s) 220
AccBSI CCGCTC 2 cut(s) 35, 3149
AccI GTMKAC 3 cut(s) 258, 1068, 4306
AccII CGCG 4 cut(s) 172, 2413, 2481, 3666
AcoI YGGCCR 4 cut(s) 297, 3674, 4068, 4922
AcsI RAATTY 5 cut(s) 402, 667, 1240, 2606, 5105
AcuI CTGAAG 2 cut(s) 1040, 1197
AcyI GRCGYC 3 cut(s) 5, 181, 1784
AdeI CACNNNGTG 2 cut(s) 1145, 3113
AflIII ACRYGT 1 cut(s) 4309
AhdI GACNNNNNGTC 2 cut(s) 655, 1458
AhlI ACTAGT 2 cut(s) 3587, 4357
AjnI CCWGG 7 cut(s) 418, 615, 1569, 2196, 2967, 3546, 3732
Alw21I GWGCWC 7 cut(s) 17, 324, 1141, 3587, 4086, 4606, 5081
Alw26I GTCTC 6 cut(s) 2659, 2845, 3078, 4141, 4705, 4830
Alw44I GTGCAC 1 cut(s) 1137
AlwNI CAGNNNCTG 3 cut(s) 44, 3389, 4232
Ama87I CYCGRG 3 cut(s) 771, 4646, 5136
ApaI GGGCCC 1 cut(s) 4006
ApaLI GTGCAC 1 cut(s) 1137
ApoI RAATTY 5 cut(s) 402, 667, 1240, 2606, 5105
ArsI GACNNNNNNTTYG 2 cut(s) 2890, 2922
Asp700I GAANNNNTTC 2 cut(s) 405, 3322
AspLEI GCGC 5 cut(s) 1786, 2483, 3181, 4341, 5011
AsuC2I CCSGG 3 cut(s) 772, 773, 1315
AsuHPI GGTGA 8 cut(s) 484, 627, 659, 1453, 1798, 2868, 3791, 4485
AsuII TTCGAA 1 cut(s) 4543
AsuNHI GCTAGC 1 cut(s) 3974
AvaI CYCGRG 3 cut(s) 771, 4646, 5136
AvaII GGWCC 4 cut(s) 908, 1307, 2533, 4625
AxyI CCTNAGG 2 cut(s) 1074, 2710
BaeGI GKGCMC 4 cut(s) 778, 1141, 2968, 4006
BaeI ACNNNNGTAYC 2 cut(s) 3468, 3501
BalI TGGCCA 2 cut(s) 3676, 4070
BamHI GGATCC 2 cut(s) 4271, 4510
BanI GGYRCC 1 cut(s) 1783
BanII GRGCYC 3 cut(s) 17, 324, 4006
BauI CACGAG 3 cut(s) 708, 1671, 2625
BbsI GAAGAC 4 cut(s) 860, 1028, 1081, 3595
Bbv12I GWGCWC 7 cut(s) 17, 324, 1141, 3587, 4086, 4606, 5081
BceAI ACGGC 7 cut(s) 312, 338, 1211, 3209, 3229, 3534, 3963
BciT130I CCWGG 7 cut(s) 420, 617, 1571, 2198, 2969, 3548, 3734
BclI TGATCA 4 cut(s) 2860, 3160, 4804, 4981
BcnI CCSGG 3 cut(s) 772, 773, 1315
BcoDI GTCTC 6 cut(s) 2659, 2845, 3078, 4141, 4705, 4830
BcuI ACTAGT 2 cut(s) 3587, 4357
BfmI CTRYAG 6 cut(s) 735, 2496, 3291, 4224, 4255, 4458
BfoI RGCGCY 2 cut(s) 1787, 4342
BfuAI ACCTGC 3 cut(s) 4118, 4642, 5002
BglII AGATCT 2 cut(s) 712, 4045
BlpI GCTNAGC 2 cut(s) 276, 3936
Bme18I GGWCC 4 cut(s) 908, 1307, 2533, 4625
BmeRI GACNNNNNGTC 2 cut(s) 655, 1458
BmeT110I CYCGRG 3 cut(s) 771, 4646, 5136
BmiI GGNNCC 6 cut(s) 1785, 2534, 3424, 4004, 4273, 4512
BmtI GCTAGC 1 cut(s) 3978
BoxI GACNNNNGTC 1 cut(s) 5157
BpiI GAAGAC 4 cut(s) 860, 1028, 1081, 3595
BpmI CTGGAG 3 cut(s) 21, 3755, 4939
Bpu1102I GCTNAGC 2 cut(s) 276, 3936
Bpu14I TTCGAA 1 cut(s) 4543
BpuMI CCSGG 3 cut(s) 772, 773, 1315
BsaAI YACGTR 1 cut(s) 5076
BsaBI GATNNNNATC 2 cut(s) 555, 2254
BsaHI GRCGYC 3 cut(s) 5, 181, 1784
BsaI GGTCTC 2 cut(s) 2659, 4830
BsaXI ACNNNNNCTCC 4 cut(s) 3075, 3105, 4286, 4316
Bse1I ACTGG 7 cut(s) 338, 965, 1585, 1961, 2738, 3760, 4758
Bse21I CCTNAGG 2 cut(s) 1074, 2710
Bse3DI GCAATG 3 cut(s) 102, 482, 3174
Bse8I GATNNNNATC 2 cut(s) 555, 2254
BseBI CCWGG 7 cut(s) 420, 617, 1571, 2198, 2969, 3548, 3734
BseJI GATNNNNATC 2 cut(s) 555, 2254
BseMI GCAATG 3 cut(s) 102, 482, 3174
BseNI ACTGG 7 cut(s) 338, 965, 1585, 1961, 2738, 3760, 4758
BseRI GAGGAG 3 cut(s) 189, 3292, 4760
BseSI GKGCMC 4 cut(s) 778, 1141, 2968, 4006
BseX3I CGGCCG 1 cut(s) 297
BseYI CCCAGC 2 cut(s) 203, 3705
BsgI GTGCAG 5 cut(s) 1605, 1642, 2597, 3135, 4218
Bsh1236I CGCG 4 cut(s) 172, 2413, 2481, 3666
Bsh1285I CGRYCG 1 cut(s) 300
BshNI GGYRCC 1 cut(s) 1783
BsiEI CGRYCG 1 cut(s) 300
BsiHKAI GWGCWC 7 cut(s) 17, 324, 1141, 3587, 4086, 4606, 5081
BsiHKCI CYCGRG 3 cut(s) 771, 4646, 5136
BsiSI CCGG 3 cut(s) 772, 1314, 4723
BsiWI CGTACG 1 cut(s) 5147
BslFI GGGAC 6 cut(s) 791, 1465, 1913, 2410, 2546, 3647
BsmAI GTCTC 6 cut(s) 2659, 2845, 3078, 4141, 4705, 4830
BsmBI CGTCTC 1 cut(s) 4141
BsmFI GGGAC 6 cut(s) 791, 1465, 1913, 2410, 2546, 3647
BsmI GAATGC 5 cut(s) 631, 1667, 1981, 2214, 4789
Bso31I GGTCTC 2 cut(s) 2659, 4830
BsoBI CYCGRG 3 cut(s) 771, 4646, 5136
Bsp119I TTCGAA 1 cut(s) 4543
Bsp120I GGGCCC 1 cut(s) 4002
Bsp1407I TGTACA 3 cut(s) 1216, 2299, 4559
Bsp1720I GCTNAGC 2 cut(s) 276, 3936
Bsp19I CCATGG 2 cut(s) 2160, 4919
Bsp68I TCGCGA 1 cut(s) 172
BspFNI CGCG 4 cut(s) 172, 2413, 2481, 3666
BspHI TCATGA 1 cut(s) 4801
BspLI GGNNCC 6 cut(s) 1785, 2534, 3424, 4004, 4273, 4512
BspMAI CTGCAG 3 cut(s) 2500, 4228, 4462
BspMI ACCTGC 3 cut(s) 4118, 4642, 5002
BspOI GCTAGC 1 cut(s) 3978
BspQI GCTCTTC 1 cut(s) 5086
BspT104I TTCGAA 1 cut(s) 4543
BspT107I GGYRCC 1 cut(s) 1783
BspTNI GGTCTC 2 cut(s) 2659, 4830
BsrBI CCGCTC 2 cut(s) 35, 3149
BsrDI GCAATG 3 cut(s) 102, 482, 3174
BsrGI TGTACA 3 cut(s) 1216, 2299, 4559
BsrI ACTGG 7 cut(s) 338, 965, 1585, 1961, 2738, 3760, 4758
BssNAI GTATAC 1 cut(s) 1069
BssNI GRCGYC 3 cut(s) 5, 181, 1784
BssSI CACGAG 3 cut(s) 708, 1671, 2625
BssT1I CCWWGG 7 cut(s) 240, 1166, 1969, 2160, 2977, 4497, 4919
Bst1107I GTATAC 1 cut(s) 1069
Bst2BI CACGAG 3 cut(s) 708, 1671, 2625
Bst2UI CCWGG 7 cut(s) 420, 617, 1571, 2198, 2969, 3548, 3734
Bst6I CTCTTC 3 cut(s) 144, 2893, 5086
BstACI GRCGYC 3 cut(s) 5, 181, 1784
BstAPI GCANNNNNTGC 6 cut(s) 467, 2342, 2963, 3695, 4232, 4384
BstAUI TGTACA 3 cut(s) 1216, 2299, 4559
BstBAI YACGTR 1 cut(s) 5076
BstBI TTCGAA 1 cut(s) 4543
BstDSI CCRYGG 4 cut(s) 2160, 3191, 3979, 4919
BstFNI CGCG 4 cut(s) 172, 2413, 2481, 3666
BstH2I RGCGCY 2 cut(s) 1787, 4342
BstHHI GCGC 5 cut(s) 1786, 2483, 3181, 4341, 5011
BstMAI GTCTC 6 cut(s) 2659, 2845, 3078, 4141, 4705, 4830
BstMCI CGRYCG 1 cut(s) 300
BstNI CCWGG 7 cut(s) 420, 617, 1571, 2198, 2969, 3548, 3734
BstNSI RCATGY 5 cut(s) 730, 864, 2306, 3743, 4313
BstPAI GACNNNNGTC 1 cut(s) 5157
BstSFI CTRYAG 6 cut(s) 735, 2496, 3291, 4224, 4255, 4458
BstSLI GKGCMC 4 cut(s) 778, 1141, 2968, 4006
BstUI CGCG 4 cut(s) 172, 2413, 2481, 3666
BstV2I GAAGAC 4 cut(s) 860, 1028, 1081, 3595
BstX2I RGATCY 7 cut(s) 712, 1876, 1964, 3295, 4045, 4271, 4510
BstXI CCANNNNNNTGG 2 cut(s) 817, 2167
BstYI RGATCY 7 cut(s) 712, 1876, 1964, 3295, 4045, 4271, 4510
BstZ17I GTATAC 1 cut(s) 1069
BstZI CGGCCG 1 cut(s) 297
Bsu36I CCTNAGG 2 cut(s) 1074, 2710
BtgI CCRYGG 4 cut(s) 2160, 3191, 3979, 4919
BtgZI GCGATG 2 cut(s) 2021, 3234
BtsI GCAGTG 2 cut(s) 2436, 2952
BtsIMutI CAGTG 8 cut(s) 972, 1345, 2190, 2436, 2745, 2952, 4235, 4264
BtuMI TCGCGA 1 cut(s) 172
BveI ACCTGC 3 cut(s) 4118, 4642, 5002
CaiI CAGNNNCTG 3 cut(s) 44, 3389, 4232
CciI TCATGA 1 cut(s) 4801
CfoI GCGC 5 cut(s) 1786, 2483, 3181, 4341, 5011
Cfr9I CCCGGG 1 cut(s) 771
CseI GACGC 5 cut(s) 170, 336, 1331, 2384, 4123
DinI GGCGCC 1 cut(s) 1785
DraI TTTAAA 1 cut(s) 1869
DraIII CACNNNGTG 2 cut(s) 1145, 3113
DriI GACNNNNNGTC 2 cut(s) 655, 1458
EaeI YGGCCR 4 cut(s) 297, 3674, 4068, 4922
EagI CGGCCG 1 cut(s) 297
Eam1104I CTCTTC 3 cut(s) 144, 2893, 5086
Eam1105I GACNNNNNGTC 2 cut(s) 655, 1458
EarI CTCTTC 3 cut(s) 144, 2893, 5086
Ecl136II GAGCTC 2 cut(s) 15, 322
EclXI CGGCCG 1 cut(s) 297
Eco130I CCWWGG 7 cut(s) 240, 1166, 1969, 2160, 2977, 4497, 4919
Eco147I AGGCCT 1 cut(s) 98
Eco24I GRGCYC 3 cut(s) 17, 324, 4006
Eco31I GGTCTC 2 cut(s) 2659, 4830
Eco32I GATATC 2 cut(s) 622, 1648
Eco47I GGWCC 4 cut(s) 908, 1307, 2533, 4625
Eco52I CGGCCG 1 cut(s) 297
Eco53kI GAGCTC 2 cut(s) 15, 322
Eco57I CTGAAG 2 cut(s) 1040, 1197
Eco81I CCTNAGG 2 cut(s) 1074, 2710
Eco88I CYCGRG 3 cut(s) 771, 4646, 5136
EcoICRI GAGCTC 2 cut(s) 15, 322
EcoO109I RGGNCCY 2 cut(s) 1492, 4003
EcoRI GAATTC 1 cut(s) 667
EcoRII CCWGG 7 cut(s) 418, 615, 1569, 2196, 2967, 3546, 3732
EcoRV GATATC 2 cut(s) 622, 1648
EcoT14I CCWWGG 7 cut(s) 240, 1166, 1969, 2160, 2977, 4497, 4919
EcoT22I ATGCAT 7 cut(s) 484, 745, 1399, 1610, 3691, 3700, 4791
EcoT38I GRGCYC 3 cut(s) 17, 324, 4006
EgeI GGCGCC 1 cut(s) 1785
EheI GGCGCC 1 cut(s) 1785
ErhI CCWWGG 7 cut(s) 240, 1166, 1969, 2160, 2977, 4497, 4919
Esp3I CGTCTC 1 cut(s) 4141
FalI AAGNNNNNCTT 4 cut(s) 498, 530, 2693, 2725
FaqI GGGAC 6 cut(s) 791, 1465, 1913, 2410, 2546, 3647
FauI CCCGC 2 cut(s) 40, 1740
FauNDI CATATG 3 cut(s) 226, 1846, 4169
FbaI TGATCA 4 cut(s) 2860, 3160, 4804, 4981
FblI GTMKAC 3 cut(s) 258, 1068, 4306
FriOI GRGCYC 3 cut(s) 17, 324, 4006
FspI TGCGCA 1 cut(s) 5010
GlaI GCGC 5 cut(s) 1785, 2482, 3180, 4340, 5010
GsaI CCCAGC 2 cut(s) 207, 3709
GsuI CTGGAG 3 cut(s) 21, 3755, 4939
HaeII RGCGCY 2 cut(s) 1787, 4342
HapII CCGG 3 cut(s) 772, 1314, 4723
HgaI GACGC 5 cut(s) 170, 336, 1331, 2384, 4123
HhaI GCGC 5 cut(s) 1786, 2483, 3181, 4341, 5011
Hin1I GRCGYC 3 cut(s) 5, 181, 1784
Hin6I GCGC 5 cut(s) 1784, 2481, 3179, 4339, 5009
HinP1I GCGC 5 cut(s) 1784, 2481, 3179, 4339, 5009
HincII GTYRAC 4 cut(s) 259, 3817, 3986, 4307
HindII GTYRAC 4 cut(s) 259, 3817, 3986, 4307
HindIII AAGCTT 4 cut(s) 278, 1179, 2584, 3501
HpaII CCGG 3 cut(s) 772, 1314, 4723
HphI GGTGA 8 cut(s) 484, 627, 659, 1453, 1798, 2868, 3791, 4485
Hpy166II GTNNAC 9 cut(s) 259, 492, 1069, 1139, 1193, 3817, 3986, 4307, 4392
Hpy8I GTNNAC 9 cut(s) 259, 492, 1069, 1139, 1193, 3817, 3986, 4307, 4392
Hpy99I CGWCG 1 cut(s) 186
HpyAV CCTTC 9 cut(s) 310, 636, 1108, 1505, 1890, 2523, 3207, 3450, 3624
HpyCH4IV ACGT 6 cut(s) 120, 1130, 2648, 3109, 4701, 5075
HpySE526I ACGT 6 cut(s) 120, 1130, 2648, 3109, 4701, 5075
Hsp92I GRCGYC 3 cut(s) 5, 181, 1784
HspAI GCGC 5 cut(s) 1784, 2481, 3179, 4339, 5009
KasI GGCGCC 1 cut(s) 1783
Ksp22I TGATCA 4 cut(s) 2860, 3160, 4804, 4981
LguI GCTCTTC 1 cut(s) 5086
MaeII ACGT 6 cut(s) 120, 1130, 2648, 3109, 4701, 5075
MbiI CCGCTC 2 cut(s) 35, 3149
MfeI CAATTG 4 cut(s) 1056, 2676, 3824, 3950
MflI RGATCY 7 cut(s) 712, 1876, 1964, 3295, 4045, 4271, 4510
MlsI TGGCCA 2 cut(s) 3676, 4070
MluNI TGGCCA 2 cut(s) 3676, 4070
Mly113I GGCGCC 1 cut(s) 1784
MlyI GAGTC 4 cut(s) 1762, 4496, 4761, 5147
MmeI TCCRAC 6 cut(s) 994, 1487, 2156, 3057, 4021, 4352
Mox20I TGGCCA 2 cut(s) 3676, 4070
Mph1103I ATGCAT 7 cut(s) 484, 745, 1399, 1610, 3691, 3700, 4791
MroXI GAANNNNTTC 2 cut(s) 405, 3322
MscI TGGCCA 2 cut(s) 3676, 4070
MslI CAYNNNNRTG 4 cut(s) 477, 4689, 4695, 5122
Msp20I TGGCCA 2 cut(s) 3676, 4070
MspA1I CMGCKG 6 cut(s) 1747, 3389, 3416, 4229, 4959, 5054
MspI CCGG 3 cut(s) 772, 1314, 4723
MunI CAATTG 4 cut(s) 1056, 2676, 3824, 3950
Mva1269I GAATGC 5 cut(s) 631, 1667, 1981, 2214, 4789
MvaI CCWGG 7 cut(s) 420, 617, 1571, 2198, 2969, 3548, 3734
MvnI CGCG 4 cut(s) 172, 2413, 2481, 3666
NarI GGCGCC 1 cut(s) 1784
NciI CCSGG 3 cut(s) 772, 773, 1315
NcoI CCATGG 2 cut(s) 2160, 4919
NdeI CATATG 3 cut(s) 226, 1846, 4169
NheI GCTAGC 1 cut(s) 3974
NlaIV GGNNCC 6 cut(s) 1785, 2534, 3424, 4004, 4273, 4512
NmeAIII GCCGAG 2 cut(s) 140, 2594
NmuCI GTSAC 7 cut(s) 647, 1343, 1853, 3448, 3526, 4491, 5159
NruI TCGCGA 1 cut(s) 172
NsbI TGCGCA 1 cut(s) 5010
NsiI ATGCAT 7 cut(s) 484, 745, 1399, 1610, 3691, 3700, 4791
NspI RCATGY 5 cut(s) 730, 864, 2306, 3743, 4313
NspV TTCGAA 1 cut(s) 4543
PaeR7I CTCGAG 2 cut(s) 4646, 5136
PagI TCATGA 1 cut(s) 4801
PasI CCCWGGG 1 cut(s) 2968
PceI AGGCCT 1 cut(s) 98
PciI ACATGT 1 cut(s) 4309
PciSI GCTCTTC 1 cut(s) 5086
PcsI WCGNNNNNNNCGW 1 cut(s) 5143
PctI GAATGC 5 cut(s) 631, 1667, 1981, 2214, 4789
PdmI GAANNNNTTC 2 cut(s) 405, 3322
Pfl23II CGTACG 1 cut(s) 5147
PflFI GACNNNGTC 2 cut(s) 2192, 3089
PflMI CCANNNNNTGG 1 cut(s) 220
PfoI TCCNGGA 2 cut(s) 2196, 3732
PleI GAGTC 4 cut(s) 1761, 4495, 4761, 5147
PluTI GGCGCC 1 cut(s) 1787
PpsI GAGTC 4 cut(s) 1761, 4495, 4761, 5147
Ppu21I YACGTR 1 cut(s) 5076
PscI ACATGT 1 cut(s) 4309
PshAI GACNNNNGTC 1 cut(s) 5157
PsiI TTATAA 1 cut(s) 1097
Psp124BI GAGCTC 2 cut(s) 17, 324
Psp6I CCWGG 7 cut(s) 418, 615, 1569, 2196, 2967, 3546, 3732
PspFI CCCAGC 2 cut(s) 203, 3705
PspGI CCWGG 7 cut(s) 418, 615, 1569, 2196, 2967, 3546, 3732
PspLI CGTACG 1 cut(s) 5147
PspN4I GGNNCC 6 cut(s) 1785, 2534, 3424, 4004, 4273, 4512
PspOMI GGGCCC 1 cut(s) 4002
PspXI VCTCGAGB 1 cut(s) 5136
PstI CTGCAG 3 cut(s) 2500, 4228, 4462
PstNI CAGNNNCTG 3 cut(s) 44, 3389, 4232
PsuI RGATCY 7 cut(s) 712, 1876, 1964, 3295, 4045, 4271, 4510
PsyI GACNNNGTC 2 cut(s) 2192, 3089
PvuII CAGCTG 3 cut(s) 3389, 4229, 4959
RruI TCGCGA 1 cut(s) 172
RseI CAYNNNNRTG 4 cut(s) 477, 4689, 4695, 5122
SacI GAGCTC 2 cut(s) 17, 324
SalI GTCGAC 2 cut(s) 257, 4305
SapI GCTCTTC 1 cut(s) 5086
SchI GAGTC 4 cut(s) 1762, 4496, 4761, 5147
SfcI CTRYAG 6 cut(s) 735, 2496, 3291, 4224, 4255, 4458
SfoI GGCGCC 1 cut(s) 1785
Sfr274I CTCGAG 2 cut(s) 4646, 5136
SfuI TTCGAA 1 cut(s) 4543
SinI GGWCC 4 cut(s) 908, 1307, 2533, 4625
SlaI CTCGAG 2 cut(s) 4646, 5136
SmaI CCCGGG 1 cut(s) 773
SmiMI CAYNNNNRTG 4 cut(s) 477, 4689, 4695, 5122
SpeI ACTAGT 2 cut(s) 3587, 4357
SseBI AGGCCT 1 cut(s) 98
SspDI GGCGCC 1 cut(s) 1783
SspI AATATT 2 cut(s) 4416, 5003
SstI GAGCTC 2 cut(s) 17, 324
StuI AGGCCT 1 cut(s) 98
StyI CCWWGG 7 cut(s) 240, 1166, 1969, 2160, 2977, 4497, 4919
TaiI ACGT 6 cut(s) 123, 1133, 2651, 3112, 4704, 5078
TatI WGTACW 7 cut(s) 1216, 2299, 2642, 2914, 4360, 4389, 4559
TauI GCSGC 3 cut(s) 791, 2416, 4540
TscAI CASTG 8 cut(s) 972, 1352, 2197, 2443, 2745, 2959, 4242, 4264
TseFI GTSAC 7 cut(s) 647, 1343, 1853, 3448, 3526, 4491, 5159
Tsp45I GTSAC 7 cut(s) 647, 1343, 1853, 3448, 3526, 4491, 5159
TspGWI ACGGA 3 cut(s) 2075, 2647, 5165
TspMI CCCGGG 1 cut(s) 771
TspRI CASTG 8 cut(s) 972, 1352, 2197, 2443, 2745, 2959, 4242, 4264
Tth111I GACNNNGTC 2 cut(s) 2192, 3089
Van91I CCANNNNNTGG 1 cut(s) 220
VneI GTGCAC 1 cut(s) 1137
VpaK11BI GGWCC 4 cut(s) 908, 1307, 2533, 4625
XapI RAATTY 5 cut(s) 402, 667, 1240, 2606, 5105
XceI RCATGY 5 cut(s) 730, 864, 2306, 3743, 4313
XcmI CCANNNNNNNNNTGG 2 cut(s) 1577, 4504
XhoI CTCGAG 2 cut(s) 4646, 5136
XmaI CCCGGG 1 cut(s) 771
XmiI GTMKAC 3 cut(s) 258, 1068, 4306
XmnI GAANNNNTTC 2 cut(s) 405, 3322
Zsp2I ATGCAT 7 cut(s) 484, 745, 1399, 1610, 3691, 3700, 4791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.