MD12G1105200.v1.1

Protein SHOOT GRAVITROPISM

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004148
Physical Location & Seq
Forward (+)
16630946 .. 16634304
3359 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1105200.v1.1.491

Sequence Viewer

Length: 588 bp
ATGGCTAAGCTTGCTAAGATTGCAACGGCCGAAATCATATCCTCGAAGGAGCTCAATGCAGACTGGCAAAGAGCTGCGTCAGCCGTTCTTGTTTCAATCGGCAGACATTTGCCAGACCTAATGATGGAAGAAATTTTTCTTCATTTGCCTGGGCCAAACTCAGCTTTACCAGCTATGGTTCAGATACTAGCAGACTTTGCTCATGCTGATGCATTGCAGTTCACCCCACGATTGAAAGGTGTGCTTTCACGGGTTGTACCTATTCTTGGAAATGTACGAGATGCCCATCGGCCTATTTTTGCTAATGCTATTAAGTGCTGGTGTCAGGCTGTTTGGCAGTATAGCCTGGATATCCCTTCGCATTCACCTTTGGATGCTGACATCATGTCATTTTTGAATTCTGTTTTTGAGTTTTTATTGAAAGTTTGGGCTGCTTCACGCGATCTAAAGGTGAAGTGTCGTGTTCGCATGTCATCTGTAGATGCATTAGGACAGATGGTTGGCCTTATTACCCGGGCACAATTAAAGGCGGCTTTACCCAGACTTGTCCCCACGATATTGGAACTTTTGTTGGTGCTGAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cnd1

Protein Analysis

196

Amino Acids

21.56

Weight (kDa)

9.33

Isoelectric Point (pI)

27.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEAT_MROH2B_1st PF23221 2 - 167 6.2e-26 MROH2B-like, HEAT repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 441
AciI CCGC 1 cut(s) 530
AcoI YGGCCR 1 cut(s) 27
AcsI RAATTY 2 cut(s) 132, 397
AfaI GTAC 2 cut(s) 258, 276
AfiI CCNNNNNNNGG 2 cut(s) 124, 266
AgsI TTSAA 4 cut(s) 96, 235, 397, 421
AhdI GACNNNNNGTC 1 cut(s) 385
AjnI CCWGG 2 cut(s) 148, 345
AluBI AGCT 5 cut(s) 10, 52, 74, 164, 173
AluI AGCT 5 cut(s) 10, 52, 74, 164, 173
Alw21I GWGCWC 1 cut(s) 54
Ama87I CYCGRG 1 cut(s) 513
AoxI GGCC 4 cut(s) 27, 152, 290, 502
ApeKI GCWGC 2 cut(s) 74, 431
ApoI RAATTY 2 cut(s) 132, 397
Asp700I GAANNNNTTC 1 cut(s) 135
AspS9I GGNCC 1 cut(s) 152
AsuC2I CCSGG 2 cut(s) 514, 515
AsuHPI GGTGA 3 cut(s) 214, 357, 463
AvaI CYCGRG 1 cut(s) 513
BaeGI GKGCMC 1 cut(s) 520
BanII GRGCYC 1 cut(s) 54
Bbv12I GWGCWC 1 cut(s) 54
BbvI GCAGC 2 cut(s) 61, 418
BccI CCATC 3 cut(s) 118, 294, 490
BceAI ACGGC 2 cut(s) 42, 68
BciT130I CCWGG 2 cut(s) 150, 347
BcnI CCSGG 2 cut(s) 514, 515
BfaI CTAG 1 cut(s) 188
BfmI CTRYAG 1 cut(s) 477
BisI GCNGC 3 cut(s) 75, 432, 531
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 3 cut(s) 76, 433, 532
Bme1390I CCNGG 4 cut(s) 150, 347, 514, 515
BmeRI GACNNNNNGTC 1 cut(s) 385
BmeT110I CYCGRG 1 cut(s) 513
BmgT120I GGNCC 1 cut(s) 152
BmrFI CCNGG 4 cut(s) 150, 347, 514, 515
BmsI GCATC 4 cut(s) 199, 271, 364, 472
Bpu1102I GCTNAGC 1 cut(s) 6
BpuMI CCSGG 2 cut(s) 514, 515
BsaBI GATNNNNATC 1 cut(s) 285
BsaJI CCNNGG 2 cut(s) 149, 513
Bsc4I CCNNNNNNNGG 2 cut(s) 124, 266
Bse1I ACTGG 1 cut(s) 68
Bse3DI GCAATG 1 cut(s) 212
Bse8I GATNNNNATC 1 cut(s) 285
BseBI CCWGG 2 cut(s) 150, 347
BseDI CCNNGG 2 cut(s) 149, 513
BseGI GGATG 1 cut(s) 379
BseJI GATNNNNATC 1 cut(s) 285
BseLI CCNNNNNNNGG 2 cut(s) 124, 266
BseMI GCAATG 1 cut(s) 212
BseMII CTCAG 1 cut(s) 174
BseNI ACTGG 1 cut(s) 68
BseSI GKGCMC 1 cut(s) 520
BseX3I CGGCCG 1 cut(s) 27
BseXI GCAGC 2 cut(s) 61, 418
Bsh1236I CGCG 1 cut(s) 441
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 4 cut(s) 29, 154, 292, 504
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 1 cut(s) 54
BsiHKCI CYCGRG 1 cut(s) 513
BsiSI CCGG 1 cut(s) 514
BslFI GGGAC 1 cut(s) 533
BslI CCNNNNNNNGG 2 cut(s) 124, 266
BsmFI GGGAC 1 cut(s) 533
BsmI GAATGC 1 cut(s) 361
BsnI GGCC 4 cut(s) 29, 154, 292, 504
BsoBI CYCGRG 1 cut(s) 513
Bsp1286I GDGCHC 2 cut(s) 54, 520
Bsp143I GATC 1 cut(s) 442
Bsp1720I GCTNAGC 1 cut(s) 6
BspACI CCGC 1 cut(s) 530
BspANI GGCC 4 cut(s) 29, 154, 292, 504
BspCNI CTCAG 1 cut(s) 173
BspFNI CGCG 1 cut(s) 441
BsrDI GCAATG 1 cut(s) 212
BsrI ACTGG 1 cut(s) 68
BssECI CCNNGG 2 cut(s) 149, 513
BssMI GATC 1 cut(s) 442
Bst2UI CCWGG 2 cut(s) 150, 347
BstAPI GCANNNNNTGC 1 cut(s) 197
BstC8I GCNNGC 1 cut(s) 12
BstDEI CTNAG 3 cut(s) 6, 15, 160
BstF5I GGATG 1 cut(s) 379
BstFNI CGCG 1 cut(s) 441
BstKTI GATC 1 cut(s) 445
BstMBI GATC 1 cut(s) 442
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 5 cut(s) 11, 20, 80, 170, 197
BstNI CCWGG 2 cut(s) 150, 347
BstNSI RCATGY 1 cut(s) 472
BstSCI CCNGG 4 cut(s) 148, 345, 512, 513
BstSFI CTRYAG 1 cut(s) 477
BstSLI GKGCMC 1 cut(s) 520
BstUI CGCG 1 cut(s) 441
BstV1I GCAGC 2 cut(s) 61, 418
BstXI CCANNNNNNTGG 1 cut(s) 559
BstZI CGGCCG 1 cut(s) 27
BsuRI GGCC 4 cut(s) 29, 154, 292, 504
BtsCI GGATG 1 cut(s) 379
Cac8I GCNNGC 1 cut(s) 12
Cfr13I GGNCC 1 cut(s) 152
Cfr9I CCCGGG 1 cut(s) 513
CseI GACGC 1 cut(s) 66
Csp6I GTAC 2 cut(s) 257, 275
CviAII CATG 3 cut(s) 203, 385, 469
CviQI GTAC 2 cut(s) 257, 275
DdeI CTNAG 3 cut(s) 6, 15, 160
DpnI GATC 1 cut(s) 444
DpnII GATC 1 cut(s) 442
DriI GACNNNNNGTC 1 cut(s) 385
EaeI YGGCCR 1 cut(s) 27
EagI CGGCCG 1 cut(s) 27
Eam1105I GACNNNNNGTC 1 cut(s) 385
Ecl136II GAGCTC 1 cut(s) 52
EclXI CGGCCG 1 cut(s) 27
Eco24I GRGCYC 1 cut(s) 54
Eco32I GATATC 1 cut(s) 352
Eco52I CGGCCG 1 cut(s) 27
Eco53kI GAGCTC 1 cut(s) 52
Eco88I CYCGRG 1 cut(s) 513
EcoICRI GAGCTC 1 cut(s) 52
EcoRI GAATTC 1 cut(s) 397
EcoRII CCWGG 2 cut(s) 148, 345
EcoRV GATATC 1 cut(s) 352
EcoT22I ATGCAT 2 cut(s) 214, 487
EcoT38I GRGCYC 1 cut(s) 54
FaeI CATG 3 cut(s) 206, 388, 472
FaiI YATR 6 cut(s) 38, 176, 204, 342, 386, 470
FalI AAGNNNNNCTT 2 cut(s) 228, 260
FaqI GGGAC 1 cut(s) 533
FatI CATG 3 cut(s) 202, 384, 468
Fnu4HI GCNGC 3 cut(s) 75, 432, 531
FokI GGATG 1 cut(s) 386
FriOI GRGCYC 1 cut(s) 54
Fsp4HI GCNGC 3 cut(s) 75, 432, 531
FspBI CTAG 1 cut(s) 188
GluI GCNGC 3 cut(s) 75, 432, 531
HaeIII GGCC 4 cut(s) 29, 154, 292, 504
HapII CCGG 1 cut(s) 514
HgaI GACGC 1 cut(s) 66
Hin1II CATG 3 cut(s) 206, 388, 472
HindIII AAGCTT 1 cut(s) 8
HpaII CCGG 1 cut(s) 514
HphI GGTGA 3 cut(s) 214, 357, 463
Hpy166II GTNNAC 1 cut(s) 222
Hpy188I TCNGA 1 cut(s) 183
Hpy8I GTNNAC 1 cut(s) 222
HpyAV CCTTC 2 cut(s) 40, 366
HpyCH4V TGCA 5 cut(s) 23, 59, 212, 217, 485
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 20, 80, 170, 197
HpyF3I CTNAG 3 cut(s) 6, 15, 160
Hsp92II CATG 3 cut(s) 206, 388, 472
Kzo9I GATC 1 cut(s) 442
LmnI GCTCC 1 cut(s) 49
Lsp1109I GCAGC 2 cut(s) 61, 418
LweI GCATC 4 cut(s) 199, 271, 364, 472
MaeI CTAG 1 cut(s) 188
MalI GATC 1 cut(s) 444
MboI GATC 1 cut(s) 442
MboII GAAGA 2 cut(s) 131, 140
MhlI GDGCHC 2 cut(s) 54, 520
MluCI AATT 3 cut(s) 132, 397, 521
MnlI CCTC 1 cut(s) 52
Mph1103I ATGCAT 2 cut(s) 214, 487
MroXI GAANNNNTTC 1 cut(s) 135
MseI TTAA 2 cut(s) 312, 524
MslI CAYNNNNRTG 1 cut(s) 207
MspI CCGG 1 cut(s) 514
MspR9I CCNGG 4 cut(s) 150, 347, 514, 515
Mva1269I GAATGC 1 cut(s) 361
MvaI CCWGG 2 cut(s) 150, 347
MvnI CGCG 1 cut(s) 441
MwoI GCNNNNNNNGC 5 cut(s) 11, 20, 80, 170, 197
NciI CCSGG 2 cut(s) 514, 515
NdeII GATC 1 cut(s) 442
NlaIII CATG 3 cut(s) 206, 388, 472
NsiI ATGCAT 2 cut(s) 214, 487
NspI RCATGY 1 cut(s) 472
PctI GAATGC 1 cut(s) 361
PdmI GAANNNNTTC 1 cut(s) 135
PkrI GCNGC 3 cut(s) 76, 433, 532
Psp124BI GAGCTC 1 cut(s) 54
Psp6I CCWGG 2 cut(s) 148, 345
PspGI CCWGG 2 cut(s) 148, 345
PspPI GGNCC 1 cut(s) 152
RsaI GTAC 2 cut(s) 258, 276
RsaNI GTAC 2 cut(s) 257, 275
RseI CAYNNNNRTG 1 cut(s) 207
SacI GAGCTC 1 cut(s) 54
SaqAI TTAA 2 cut(s) 312, 524
SatI GCNGC 3 cut(s) 75, 432, 531
Sau3AI GATC 1 cut(s) 442
Sau96I GGNCC 1 cut(s) 152
ScrFI CCNGG 4 cut(s) 150, 347, 514, 515
SduI GDGCHC 2 cut(s) 54, 520
SfaNI GCATC 4 cut(s) 199, 271, 364, 472
SfcI CTRYAG 1 cut(s) 477
SmaI CCCGGG 1 cut(s) 515
SmiMI CAYNNNNRTG 1 cut(s) 207
Sse9I AATT 3 cut(s) 132, 397, 521
SsiI CCGC 1 cut(s) 530
SspMI CTAG 1 cut(s) 188
SstI GAGCTC 1 cut(s) 54
StyD4I CCNGG 4 cut(s) 148, 345, 512, 513
TaqI TCGA 1 cut(s) 44
TasI AATT 3 cut(s) 132, 397, 521
TauI GCSGC 1 cut(s) 533
Tru1I TTAA 2 cut(s) 312, 524
Tru9I TTAA 2 cut(s) 312, 524
TseI GCWGC 2 cut(s) 74, 431
TspDTI ATGAA 1 cut(s) 131
TspMI CCCGGG 1 cut(s) 513
XapI RAATTY 2 cut(s) 132, 397
XceI RCATGY 1 cut(s) 472
XmaI CCCGGG 1 cut(s) 513
XmnI GAANNNNTTC 1 cut(s) 135
XspI CTAG 1 cut(s) 188
Zsp2I ATGCAT 2 cut(s) 214, 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.