Rw3G015450

Protein SHOOT GRAVITROPISM

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0004148
Physical Location & Seq
Forward (+)
15925971 .. 15947956
21986 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G015450.1

Sequence Viewer

Length: 5145 bp
ATGGCGTCGTCCAGCTCTGGAAGTTCAATTGCAGCTCCAGAGGCCGTTCAAGTTCTGGTTTCGCTGCTCGCAGATGAATCCCCCAATGTCAGACAAGCCTCCATCGCCTCCCTCAGAGAAGTTGCCGCCCTCAATCCTGTTATAGTTCTTGACTGTTGCTCGGCCGTTTCGCGAGGAGGACGAAGGCGATTTGGTAACATGGCTGGACTTTTCCAAGTAATGTCATATGGAGTTGGAGCTTTGGACAAGAATGACGTCGATTCTGTTTTCATGACTAAGCTTGCCAAGATTGCCACGGCCGAGATTATCTCCTCCAAGGAGCTCCATACAGACTGGCAAAGGGCTGCATCCGGCCTTCTTGTTTCGATTGGCTTGCATTTGCCTGACCTGATGATGGACGAAATATTTCTCCATTTGCCGGGGCCAAATTCAGCTATACCAGCTATGGTTCAGATACTAGCAGACTTTGCCTGCGCTGATGCATTCCAGTTCACACCACGATTGAAAGATGTGCTTTCGCGGGTTCTACCTATTCTAGGAAATGTGCGTGATGCCCACCGGCCAATTTTTGCTAATGCTATTAAGTGTTGGTGTCAGGCTGTTTGGCAATATAGTCTGGATAACCCTTCTTACCCATCTCTGGATAGTGACATAATGTCATTTCTGAATTCTGTTTTTGAGCTTTTATTAAGAGTTTGGGCAGCTTCAAGGGATCTAAAGGTTAGGATATCTTCTGTAGAAGCATTGGGTCAGATGGTTGGCCTTATTCCCCGGACCCAATTAAAGGCTGCTCTAAATCGGCTTGTCCCCACGATATTGGAATTGTATAAAAGAGATCAAGATATTTCGTTTTTGGCAACTTGTAGTCTTCACAATCTCTTAAATGCTTCTGTACAGTCAGATAGTGGTCCTCCTTTACTTGAATTTGAGGAGCTCACCATCATTCTATCAACACTCCTTCCAGTGGTCTGCATCCATAATGACAACAAAGAAAACTCAGATTTCTCAGTTGGATTGAAGACATATAATGAAGTTCAGCGTTGCTTCCTGACAGTTGGTTTGGTATACCCTGAAGATTTATTTGTTTTCCTTCTTAATAAATGCAAATTGAAGGAAGAACCTTTGGCGTTTGGTGCACTGTGTGTTCTAAAGCATCTATTACCCAGGCTGTCTGAAGCTTGGCACAATAAACGACCCTTACTTGTTGAAGCTGTCAAATCCTTGCTAGATGAGCAAAACTTAGGTGTCCGAAAGGTGCTTTCAGAGTTGATTGTCGTTATGGCTTCGCATTGTTACTTAGTTGGTCCATCTGGGGAGCTGTTTGTTGAATATCTTGTGCGTCATTGTGCTTTAACAGACAATGATAGACATGACTTTGAGAGGTCTAAGGATGTTTCAGGAAACACCTATGTTCCGTTCCAATACAAGAGATCGGAGGTGAAAATTGGGACACTTAGTCCAATGGAGTTGAGGGCAATAAGTGAAAAAAGTCTTCTGTTGCTGACCATTACGATCCCCGAAATGGAGCATATATTATGGCCTTTTCTGTTGAAGATGATTATTCCACGAGCTTATACGGGTGCAGTTGCTATGGTTTGCAGATGCATCTCTGAGTTGTGCAGGCATAGATCTTCTAATAGCGATACTATGGTGAAGGAGTGCAAAGCTCGTGCTGATATACCAAATCCCGAGGAGCTTTTTGTTCGGTTGGTAGTACTTCTGCATGATCCTTTAGCGAGGGAGCAGCTGGCTAGTCAGATTTTGACAGTACTTTGTTATCTGGCACCACTTTTTCCAAAAAATGTCAGCTTGTTTTGGCAAGATGAGATTCCAAAATTGAAGGCATATGTTAGTGACACAGAAGATCTAAAGCAGGATCCTTCTTATCAAGAAACTTGGGACGACATGATAATCAATTTTTTTGCAGAGTCTTTGGATGTGATTCATGATGCTGCCTGGGTGATATCTCTTGGAGATGCTGTTACCAAACAATATGAACTTTATACATCTGATGATGAACACTCTGCACTTCTTCATCGCTGCTTTGGTGTGTTGCTTCAAAAAGTTAATGACAGGGCATATGTCCGTGATAAGATAGATTGGATGTATAAACAAGCAGACATCACAATTCCAACAAATAGGCTTGGTCTGGCAAAAGCAATGGGGTTGGTTGCAGCATCCCACTTGGACACAGTCCTGGAAAAGCTGAAGGGGATTCTGGATAATGTTGGGCAAAGTATCTTTCGAAGATTCCTATCTTTTTTCTCGGACGATTTTAAAACTGAAGAGGCTGATGATATACATGCTGCTTTGGCTCTAATGTATGGATATGCTGCAAAATATGCTCCATCAACAGTCATTGAAGCCAGAATAGATGCACTTGTTGGGACTAATATGCTCTCACGGCTTCTTCACGTGCGCGACCCTACTGCAAAGCAGGCCGTTATTACTGCTATCGATCTTCTAGGTCGTGCTGTCATTAATGCTGCGGAAAATGGTTCATCATTTCCATTAAAGAAAAGGGACCAAATGCTAGACTATACATTAACTCTGATGGGCCGAGATGAGAATGAAAACCTTTCAGATTCTACTCTTGAACTTCTGGACACACAAGCCCGTGCTTTAAGTGCATGCACCACGCTGGTCTCTGTAGAGCCAAAACTAACAATTGAAACCAGAAACCATGTGTTGAAGGCGACCTTAGGGTTCTTTGCTTTACCAAATGATCCAGCAGATGTCATCGATCCCCTTATTGACAACCTTATCACTCTATTGTGTGCAATTCTTCTTACAAGTGGAGAAGATGGAAGAAGTCGAGCAGAGCAGTTACTGCACATCTTGAGACAAATTGATCAATATGTTTCTTCAGCTACTGATTATCAAAGAAGAAGAGGTTGTCATGCAGTACATGAGATGCTTCTCAAGTTTCGGACAGTTTGCATCACTGGACATTGTGCCCTTGGCTGTCAAGGAAGTTGTACGCACATCAAGCCAATTGACCGTAATTTGCATGGGAACTTTTCCAACTTACCATCGGCATTTGTATTGCCAAGTCGTGAGGCCTTGTCTTTGGGAGACAGGGTTATTACATATCTCCCCCGCTGTGCAGACACCAATGCTGAAGTTAGAAAAGTCTCTGCTCAGATTCTTGATCAACTTTTTAGCATCTCACTTTCACTTCAGCGGCCTGCATCTTCCACTTATGGTGTGGACATAGAAGTGTCCTACAGTGCTTTATCATCCCTCGAGGATGTCATAGCTATTTTGAGGAGTGATGCTTCTATAGATCCATCTGAGGTTTTCAACAGAGTCATCTCTTCTGTTTGTTTATTACTCACAAAGAATGAGCTTGTAGCTACCCTGCATGGTTGCACAGCTGCTATTTGCGATAAGGTCAAGCAATCAGCTGAAGGGGCGATTCAAGCCGTTATTGAGTTTGTCACAAGAAGGGGGAATGAGCTTAGTGAAAGTGATGTTTCAAGGACAACGCAAGCTCTGTTGACAGCTACTGCTCATGTTACAGAGAAGCATTTACGTCAGGAAACTCTTGCTGCCATTTCTTCTCTTGCCGAGAGCACTAGTACGAAAGTTGTCTTCAATGAAGTTTTGGCCACAGCCGGAAGGGATATAGTAACTAAGGATATTTCCAGACTACGTGGTGGTTGGCCAATACATGATGCTTTTTATGCATTTTCCCAGCATACAGTTCTTTCATTTTCATTCCTGGAACATGTGATATGTGTCCTTGATCAGTATCCCATCCTTAGAGCTGATTCAGAGAAAGGAGACTATTCTAGCCATTCAGTTGATGGTCAAATGGAGGATGACATTCTTCAAGCAACCATTATTGCTCTCACAGCATTTTTCAGAGGTGGTGGCAGAATAGGAAAAAAAGCAGTTCAACAAAACTATGCTTCAGTTCTTGCTGAGCTCACTCTTCAATTGGGAAGCTGTCATGGTCTGGCCAAGTGTGGTCAGCATGAACCATTACGGGCTCTTCTAACTGCATTCCAAGTGTTCTGTGAATGTGTTGGAGATCTTGAGATGGGAAAGATTTTGGCTCGAGATGGAGAGCAAAATGAAAATGAGAGGTGGATCAATCTTATTGGAGACATAGCAGGCTGCATTTCTATAAAGAGACCGAAAGAGGTTCAACGGATATGTGTAATTTTTAGTAAATCATTAGATCGACACCAAAGATTCCAAAGGGAAGCTGCAGCTGCTGCATTGTCAGAGTTTATCCGCTACAGTGATGGTTTTGGCTCCCTATTGGAGCAGATGGTGGAGGTGTTGTGTCGACATGTATCAGATGAATCGCCAACTGTCAGGCGCTTGTGTTTGAGAGGATTAGTACAGATACCGTCTATTCAAATGCTTCAGTATACAACTCAAGTTCTGGGCGTAATCTTAGCTTTACTAGATGATTCTGATGAATCTGTGCAATTAACTGCAGTTACTTGCTTACTTACGATGCTCGAGTCATCACCCAGTGATGCGGTGGATCCCATTTTGCTTAATCTTTCTGTACGGCTTCGCAATCTTCAGATAAGCATGAATCCAGAGATGCGAGCCAATGCATTTTCAGCTTTAGGATCACTTTGCAACTATGGAATTGGGGCACAACATGAGGCATTTCGTGAGCAGGTACATGCTATTATGCCACGCTTGGTTCTGCATTTGCATGATAACGATGTTATCGTTCGACAGGCTTGCCGGAGTACTCTAAAACAAATTGCCCCCTTGCTGGAAATGGAAGGATTATTTCCCTTGTTCAACATGCATTGTTTCAATTATGACCACCGAACTGACTACGAGGACTTTGTCAGAGAACTCACAAAGCAATTTGCCCAGCATCTTCCCTCAAGAGTTGATTCTTACATGGCATCGACAATTCAGGCTTTAGATGCACCTTGGCCAATAATTCAGGCAAATGCTATATACTTCTCCAGCTGTATGCTATCTCTGTCAGATGATCAGCACATTTTGACTCTCTATTATCCACAGGTATTTGGACCATTAGTAGGAAAATTGAGCAAGTCAGCAGATGCAAGCGTTAGAGCAACGTGCTCTTCCGCCCTTGGTTTGCTGTTAAAATCCTCAAAGTCAATATCATGGAAAGCAGCTAGAGCGGATCGCGTGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cnd1

Protein Analysis

1714

Amino Acids

190.12

Weight (kDa)

5.76

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEAT_MROH2B_1st PF23221 45 - 253 7e-29 MROH2B-like, HEAT repeats
HEAT_Maestro_2 PF23210 257 - 902 1.1e-97 MROH2B-like, HEAT repeats
HEAT_Maestro PF21047 1022 - 1199 7.4e-09 Maestro, HEAT repeats
TOR1L1_N PF24714 1409 - 1579 3.2e-07 TORTIFOLIA1 N-terminal
Cnd1 PF12717 1421 - 1499 9.9e-06 non-SMC mitotic condensation complex subunit 1
HEAT_MROH2B_C PF23227 1428 - 1696 1.2e-42 MROH2B-like, HEAT repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 258
AbsI CCTCGAGG 1 cut(s) 3245
Acc36I ACCTGC 1 cut(s) 4633
AccB1I GGYRCC 1 cut(s) 1783
AccBSI CCGCTC 1 cut(s) 5129
AccI GTMKAC 3 cut(s) 1065, 4297, 4382
AccII CGCG 4 cut(s) 172, 520, 2421, 5136
AciI CCGC 9 cut(s) 126, 520, 2489, 3100, 3184, 4243, 4496, 5073, 5129
AcoI YGGCCR 7 cut(s) 162, 297, 560, 3609, 3665, 3963, 4913
AcsI RAATTY 3 cut(s) 427, 667, 923
AcvI CACGTG 1 cut(s) 2416
AcyI GRCGYC 2 cut(s) 5, 255
AdeI CACNNNGTG 2 cut(s) 1142, 4490
AfiI CCNNNNNNNGG 9 cut(s) 394, 418, 536, 640, 784, 964, 1522, 4744, 5021
AflIII ACRYGT 2 cut(s) 3730, 4300
AhdI GACNNNNNGTC 2 cut(s) 655, 1455
AhlI ACTAGT 1 cut(s) 3578
AjnI CCWGG 4 cut(s) 1163, 1955, 2196, 3723
Alw21I GWGCWC 6 cut(s) 324, 936, 1138, 3578, 3933, 5069
Alw26I GTCTC 7 cut(s) 2650, 2836, 3069, 3139, 3780, 4104, 4132
Alw44I GTGCAC 1 cut(s) 1134
AlwNI CAGNNNCTG 4 cut(s) 2830, 2873, 3509, 4223
Ama87I CYCGRG 4 cut(s) 1688, 3245, 4062, 4475
ApaLI GTGCAC 1 cut(s) 1134
ApoI RAATTY 3 cut(s) 427, 667, 923
ArsI GACNNNNNNTTYG 2 cut(s) 1042, 1074
AseI ATTAAT 1 cut(s) 2481
Asp700I GAANNNNTTC 1 cut(s) 405
AspLEI GCGC 3 cut(s) 476, 2421, 4332
AspS9I GGNCC 7 cut(s) 422, 774, 908, 1304, 2524, 2557, 5012
AsuC2I CCSGG 2 cut(s) 420, 772
AsuHPI GGTGA 5 cut(s) 928, 1450, 1663, 1972, 4476
AsuII TTCGAA 1 cut(s) 2245
AvaI CYCGRG 4 cut(s) 1688, 3245, 4062, 4475
AvaII GGWCC 5 cut(s) 774, 908, 1304, 2524, 5012
AxyI CCTNAGG 1 cut(s) 2701
BaeGI GKGCMC 3 cut(s) 1138, 2959, 4621
BaeI ACNNNNGTAYC 2 cut(s) 1635, 1668
BalI TGGCCA 4 cut(s) 3611, 3667, 3965, 4915
BamHI GGATCC 2 cut(s) 1876, 4501
BanI GGYRCC 1 cut(s) 1783
BanII GRGCYC 4 cut(s) 324, 936, 3933, 3997
BauI CACGAG 2 cut(s) 1566, 1668
BbrPI CACGTG 1 cut(s) 2416
BbsI GAAGAC 4 cut(s) 860, 1025, 1484, 3586
Bbv12I GWGCWC 6 cut(s) 324, 936, 1138, 3578, 3933, 5069
BceAI ACGGC 7 cut(s) 29, 149, 312, 2420, 2426, 3410, 4544
BcgI CGANNNNNNTGC 6 cut(s) 355, 389, 2411, 2445, 4692, 4726
BciT130I CCWGG 4 cut(s) 1165, 1957, 2198, 3725
BciVI GTATCC 1 cut(s) 3765
BclI TGATCA 4 cut(s) 2851, 3151, 3748, 4972
BcnI CCSGG 2 cut(s) 420, 772
BcoDI GTCTC 7 cut(s) 2650, 2836, 3069, 3139, 3780, 4104, 4132
BcuI ACTAGT 1 cut(s) 3578
BfmI CTRYAG 7 cut(s) 735, 2649, 3226, 3282, 4215, 4246, 4449
BfoI RGCGCY 1 cut(s) 4333
BfuAI ACCTGC 1 cut(s) 4633
BfuI GTATCC 1 cut(s) 3765
BglII AGATCT 3 cut(s) 1628, 1864, 4036
BlpI GCTNAGC 1 cut(s) 3927
BmcAI AGTACT 3 cut(s) 1716, 1770, 4720
Bme1390I CCNGG 6 cut(s) 420, 772, 1165, 1957, 2198, 3725
Bme18I GGWCC 5 cut(s) 774, 908, 1304, 2524, 5012
BmeRI GACNNNNNGTC 2 cut(s) 655, 1455
BmeT110I CYCGRG 4 cut(s) 1688, 3245, 4062, 4475
BmgT120I GGNCC 7 cut(s) 422, 774, 908, 1304, 2524, 2557, 5012
BmiI GGNNCC 7 cut(s) 423, 776, 1785, 1878, 2525, 4264, 4503
BmrFI CCNGG 6 cut(s) 420, 772, 1165, 1957, 2198, 3725
BmrI ACTGGG 1 cut(s) 4482
BmuI ACTGGG 1 cut(s) 4482
BpiI GAAGAC 4 cut(s) 860, 1025, 1484, 3586
BplI GAGNNNNNCTC 2 cut(s) 293, 325
BpmI CTGGAG 2 cut(s) 21, 4930
Bpu1102I GCTNAGC 1 cut(s) 3927
Bpu14I TTCGAA 1 cut(s) 2245
BpuEI CTTGAG 5 cut(s) 2860, 2906, 4061, 4374, 4846
BpuMI CCSGG 2 cut(s) 420, 772
Bsa29I ATCGAT 2 cut(s) 2457, 2742
BsaAI YACGTR 2 cut(s) 2416, 3656
BsaBI GATNNNNATC 2 cut(s) 2254, 3753
BsaHI GRCGYC 2 cut(s) 5, 255
BsaI GGTCTC 2 cut(s) 2650, 4132
BsaXI ACNNNNNCTCC 6 cut(s) 1965, 1995, 3066, 3096, 4277, 4307
Bsc4I CCNNNNNNNGG 9 cut(s) 394, 418, 536, 640, 784, 964, 1522, 4744, 5021
Bse118I RCCGGY 1 cut(s) 558
Bse1I ACTGG 5 cut(s) 338, 487, 962, 2950, 4488
Bse21I CCTNAGG 1 cut(s) 2701
Bse3DI GCAATG 1 cut(s) 2166
Bse8I GATNNNNATC 2 cut(s) 2254, 3753
BseBI CCWGG 4 cut(s) 1165, 1957, 2198, 3725
BseCI ATCGAT 2 cut(s) 2457, 2742
BseJI GATNNNNATC 2 cut(s) 2254, 3753
BseLI CCNNNNNNNGG 9 cut(s) 394, 418, 536, 640, 784, 964, 1522, 4744, 5021
BseMI GCAATG 1 cut(s) 2166
BseMII CTCAG 7 cut(s) 127, 1011, 1020, 1602, 3155, 3285, 3918
BseNI ACTGG 5 cut(s) 338, 487, 962, 2950, 4488
BseRI GAGGAG 5 cut(s) 189, 301, 944, 1706, 3283
BseSI GKGCMC 3 cut(s) 1138, 2959, 4621
BseX3I CGGCCG 2 cut(s) 162, 297
BseYI CCCAGC 2 cut(s) 3696, 4848
BsgI GTGCAG 5 cut(s) 1602, 1639, 2010, 2816, 3126
Bsh1236I CGCG 4 cut(s) 172, 520, 2421, 5136
Bsh1285I CGRYCG 2 cut(s) 165, 300
BshNI GGYRCC 1 cut(s) 1783
BshVI ATCGAT 2 cut(s) 2457, 2742
BsiEI CGRYCG 2 cut(s) 165, 300
BsiHKAI GWGCWC 6 cut(s) 324, 936, 1138, 3578, 3933, 5069
BsiHKCI CYCGRG 4 cut(s) 1688, 3245, 4062, 4475
BsiSI CCGG 6 cut(s) 351, 419, 559, 772, 3618, 4714
BslFI GGGAC 5 cut(s) 791, 1462, 1913, 2401, 2537
BslI CCNNNNNNNGG 9 cut(s) 394, 418, 536, 640, 784, 964, 1522, 4744, 5021
BsmAI GTCTC 7 cut(s) 2650, 2836, 3069, 3139, 3780, 4104, 4132
BsmFI GGGAC 5 cut(s) 791, 1462, 1913, 2401, 2537
BsmI GAATGC 2 cut(s) 482, 4007
Bso31I GGTCTC 2 cut(s) 2650, 4132
BsoBI CYCGRG 4 cut(s) 1688, 3245, 4062, 4475
Bsp119I TTCGAA 1 cut(s) 2245
Bsp1286I GDGCHC 9 cut(s) 324, 936, 1138, 2959, 3578, 3933, 3997, 4621, 5069
Bsp1407I TGTACA 1 cut(s) 892
Bsp1720I GCTNAGC 1 cut(s) 3927
Bsp68I TCGCGA 1 cut(s) 172
BspACI CCGC 9 cut(s) 126, 520, 2489, 3100, 3184, 4243, 4496, 5073, 5129
BspCNI CTCAG 7 cut(s) 126, 1010, 1019, 1603, 3154, 3286, 3919
BspDI ATCGAT 2 cut(s) 2457, 2742
BspFNI CGCG 4 cut(s) 172, 520, 2421, 5136
BspHI TCATGA 2 cut(s) 270, 1945
BspLI GGNNCC 7 cut(s) 423, 776, 1785, 1878, 2525, 4264, 4503
BspMAI CTGCAG 2 cut(s) 4219, 4453
BspMI ACCTGC 1 cut(s) 4633
BspQI GCTCTTC 2 cut(s) 4002, 5074
BspT104I TTCGAA 1 cut(s) 2245
BspT107I GGYRCC 1 cut(s) 1783
BspTNI GGTCTC 2 cut(s) 2650, 4132
BsrBI CCGCTC 1 cut(s) 5129
BsrDI GCAATG 1 cut(s) 2166
BsrFI RCCGGY 1 cut(s) 558
BsrGI TGTACA 1 cut(s) 892
BsrI ACTGG 5 cut(s) 338, 487, 962, 2950, 4488
BssAI RCCGGY 1 cut(s) 558
BssNAI GTATAC 2 cut(s) 1066, 4383
BssNI GRCGYC 2 cut(s) 5, 255
BssSI CACGAG 2 cut(s) 1566, 1668
BssT1I CCWWGG 4 cut(s) 315, 2959, 4910, 5077
Bst1107I GTATAC 2 cut(s) 1066, 4383
Bst2BI CACGAG 2 cut(s) 1566, 1668
Bst2UI CCWGG 4 cut(s) 1165, 1957, 2198, 3725
Bst6I CTCTTC 6 cut(s) 2280, 2884, 3322, 3942, 4002, 5074
BstACI GRCGYC 2 cut(s) 5, 255
BstAPI GCANNNNNTGC 4 cut(s) 467, 2342, 2830, 4223
BstAUI TGTACA 1 cut(s) 892
BstBAI YACGTR 2 cut(s) 2416, 3656
BstBI TTCGAA 1 cut(s) 2245
BstDSI CCRYGG 1 cut(s) 294
BstENI CCTNNNNNAGG 1 cut(s) 534
BstFNI CGCG 4 cut(s) 172, 520, 2421, 5136
BstH2I RGCGCY 1 cut(s) 4333
BstHHI GCGC 3 cut(s) 476, 2421, 4332
BstMAI GTCTC 7 cut(s) 2650, 2836, 3069, 3139, 3780, 4104, 4132
BstMCI CGRYCG 2 cut(s) 165, 300
BstNI CCWGG 4 cut(s) 1165, 1957, 2198, 3725
BstNSI RCATGY 6 cut(s) 2306, 2634, 3734, 4304, 4652, 4780
BstSCI CCNGG 6 cut(s) 418, 770, 1163, 1955, 2196, 3723
BstSFI CTRYAG 7 cut(s) 735, 2649, 3226, 3282, 4215, 4246, 4449
BstSLI GKGCMC 3 cut(s) 1138, 2959, 4621
BstUI CGCG 4 cut(s) 172, 520, 2421, 5136
BstV2I GAAGAC 4 cut(s) 860, 1025, 1484, 3586
BstX2I RGATCY 7 cut(s) 712, 1628, 1864, 1876, 3286, 4036, 4501
BstXI CCANNNNNNTGG 1 cut(s) 817
BstYI RGATCY 7 cut(s) 712, 1628, 1864, 1876, 3286, 4036, 4501
BstZ17I GTATAC 2 cut(s) 1066, 4383
BstZI CGGCCG 2 cut(s) 162, 297
Bsu15I ATCGAT 2 cut(s) 2457, 2742
Bsu36I CCTNAGG 1 cut(s) 2701
BsuI GTATCC 1 cut(s) 3765
BsuTUI ATCGAT 2 cut(s) 2457, 2742
BtgI CCRYGG 1 cut(s) 294
BtgZI GCGATG 2 cut(s) 88, 2021
BtsIMutI CAGTG 6 cut(s) 969, 1136, 2943, 3235, 4255, 4495
BtuMI TCGCGA 1 cut(s) 172
BveI ACCTGC 1 cut(s) 4633
CaiI CAGNNNCTG 4 cut(s) 2830, 2873, 3509, 4223
CciI TCATGA 2 cut(s) 270, 1945
CfoI GCGC 3 cut(s) 476, 2421, 4332
Cfr10I RCCGGY 1 cut(s) 558
Cfr13I GGNCC 7 cut(s) 422, 774, 908, 1304, 2524, 2557, 5012
ClaI ATCGAT 2 cut(s) 2457, 2742
CseI GACGC 1 cut(s) 1328
DraI TTTAAA 1 cut(s) 2278
DraIII CACNNNGTG 2 cut(s) 1142, 4490
DriI GACNNNNNGTC 2 cut(s) 655, 1455
EaeI YGGCCR 7 cut(s) 162, 297, 560, 3609, 3665, 3963, 4913
EagI CGGCCG 2 cut(s) 162, 297
Eam1104I CTCTTC 6 cut(s) 2280, 2884, 3322, 3942, 4002, 5074
Eam1105I GACNNNNNGTC 2 cut(s) 655, 1455
EarI CTCTTC 6 cut(s) 2280, 2884, 3322, 3942, 4002, 5074
EciI GGCGGA 1 cut(s) 5062
Ecl136II GAGCTC 3 cut(s) 322, 934, 3931
EclXI CGGCCG 2 cut(s) 162, 297
Eco130I CCWWGG 4 cut(s) 315, 2959, 4910, 5077
Eco147I AGGCCT 1 cut(s) 3062
Eco24I GRGCYC 4 cut(s) 324, 936, 3933, 3997
Eco31I GGTCTC 2 cut(s) 2650, 4132
Eco32I GATATC 2 cut(s) 729, 1965
Eco47I GGWCC 5 cut(s) 774, 908, 1304, 2524, 5012
Eco52I CGGCCG 2 cut(s) 162, 297
Eco53kI GAGCTC 3 cut(s) 322, 934, 3931
Eco72I CACGTG 1 cut(s) 2416
Eco81I CCTNAGG 1 cut(s) 2701
Eco88I CYCGRG 4 cut(s) 1688, 3245, 4062, 4475
EcoICRI GAGCTC 3 cut(s) 322, 934, 3931
EcoNI CCTNNNNNAGG 1 cut(s) 534
EcoRI GAATTC 1 cut(s) 667
EcoRII CCWGG 4 cut(s) 1163, 1955, 2196, 3723
EcoRV GATATC 2 cut(s) 729, 1965
EcoT14I CCWWGG 4 cut(s) 315, 2959, 4910, 5077
EcoT22I ATGCAT 5 cut(s) 484, 1607, 3691, 4579, 4782
EcoT38I GRGCYC 4 cut(s) 324, 936, 3933, 3997
ErhI CCWWGG 4 cut(s) 315, 2959, 4910, 5077
FalI AAGNNNNNCTT 4 cut(s) 498, 530, 2684, 2716
FaqI GGGAC 5 cut(s) 791, 1462, 1913, 2401, 2537
FauI CCCGC 2 cut(s) 513, 3107
FauNDI CATATG 3 cut(s) 226, 1846, 2080
FbaI TGATCA 4 cut(s) 2851, 3151, 3748, 4972
FblI GTMKAC 3 cut(s) 1065, 4297, 4382
FriOI GRGCYC 4 cut(s) 324, 936, 3933, 3997
GlaI GCGC 3 cut(s) 475, 2420, 4331
GsaI CCCAGC 2 cut(s) 3700, 4852
GsuI CTGGAG 2 cut(s) 21, 4930
HaeII RGCGCY 1 cut(s) 4333
HapII CCGG 6 cut(s) 351, 419, 559, 772, 3618, 4714
HgaI GACGC 1 cut(s) 1328
HhaI GCGC 3 cut(s) 476, 2421, 4332
Hin1I GRCGYC 2 cut(s) 5, 255
Hin6I GCGC 3 cut(s) 474, 2419, 4330
HinP1I GCGC 3 cut(s) 474, 2419, 4330
HincII GTYRAC 2 cut(s) 3501, 4298
HindII GTYRAC 2 cut(s) 3501, 4298
HindIII AAGCTT 2 cut(s) 278, 1176
HpaII CCGG 6 cut(s) 351, 419, 559, 772, 3618, 4714
HphI GGTGA 5 cut(s) 928, 1450, 1663, 1972, 4476
Hpy166II GTNNAC 7 cut(s) 492, 1066, 1136, 3211, 3501, 4298, 4383
Hpy8I GTNNAC 7 cut(s) 492, 1066, 1136, 3211, 3501, 4298, 4383
Hpy99I CGWCG 2 cut(s) 10, 260
HpyCH4IV ACGT 5 cut(s) 255, 2415, 3535, 3655, 5063
HpySE526I ACGT 5 cut(s) 255, 2415, 3535, 3655, 5063
Hsp92I GRCGYC 2 cut(s) 5, 255
HspAI GCGC 3 cut(s) 474, 2419, 4330
Ksp22I TGATCA 4 cut(s) 2851, 3151, 3748, 4972
LguI GCTCTTC 2 cut(s) 4002, 5074
MaeII ACGT 5 cut(s) 255, 2415, 3535, 3655, 5063
MbiI CCGCTC 1 cut(s) 5129
MfeI CAATTG 4 cut(s) 27, 2667, 2994, 3941
MflI RGATCY 7 cut(s) 712, 1628, 1864, 1876, 3286, 4036, 4501
MhlI GDGCHC 9 cut(s) 324, 936, 1138, 2959, 3578, 3933, 3997, 4621, 5069
MlsI TGGCCA 4 cut(s) 3611, 3667, 3965, 4915
MluNI TGGCCA 4 cut(s) 3611, 3667, 3965, 4915
MlyI GAGTC 4 cut(s) 1937, 3318, 4487, 4981
MmeI TCCRAC 5 cut(s) 214, 991, 2156, 3048, 4012
Mox20I TGGCCA 4 cut(s) 3611, 3667, 3965, 4915
Mph1103I ATGCAT 5 cut(s) 484, 1607, 3691, 4579, 4782
MroXI GAANNNNTTC 1 cut(s) 405
MscI TGGCCA 4 cut(s) 3611, 3667, 3965, 4915
MslI CAYNNNNRTG 2 cut(s) 3218, 4680
Msp20I TGGCCA 4 cut(s) 3611, 3667, 3965, 4915
MspA1I CMGCKG 7 cut(s) 1747, 3102, 3184, 3377, 3407, 4220, 4950
MspI CCGG 6 cut(s) 351, 419, 559, 772, 3618, 4714
MspR9I CCNGG 6 cut(s) 420, 772, 1165, 1957, 2198, 3725
MunI CAATTG 4 cut(s) 27, 2667, 2994, 3941
Mva1269I GAATGC 2 cut(s) 482, 4007
MvaI CCWGG 4 cut(s) 1165, 1957, 2198, 3725
MvnI CGCG 4 cut(s) 172, 520, 2421, 5136
NciI CCSGG 2 cut(s) 420, 772
NdeI CATATG 3 cut(s) 226, 1846, 2080
NlaIV GGNNCC 7 cut(s) 423, 776, 1785, 1878, 2525, 4264, 4503
NmeAIII GCCGAG 4 cut(s) 140, 325, 2585, 3595
NmuCI GTSAC 3 cut(s) 647, 1853, 3439
NruI TCGCGA 1 cut(s) 172
NsiI ATGCAT 5 cut(s) 484, 1607, 3691, 4579, 4782
NspI RCATGY 6 cut(s) 2306, 2634, 3734, 4304, 4652, 4780
NspV TTCGAA 1 cut(s) 2245
PaeI GCATGC 1 cut(s) 2634
PaeR7I CTCGAG 3 cut(s) 3245, 4062, 4475
PagI TCATGA 2 cut(s) 270, 1945
PceI AGGCCT 1 cut(s) 3062
PciI ACATGT 2 cut(s) 3730, 4300
PciSI GCTCTTC 2 cut(s) 4002, 5074
PcsI WCGNNNNNNNCGW 1 cut(s) 4695
PctI GAATGC 2 cut(s) 482, 4007
PdmI GAANNNNTTC 1 cut(s) 405
PflFI GACNNNGTC 2 cut(s) 2192, 4820
PfoI TCCNGGA 2 cut(s) 2196, 3723
PleI GAGTC 4 cut(s) 1936, 3317, 4486, 4981
PmaCI CACGTG 1 cut(s) 2416
PmlI CACGTG 1 cut(s) 2416
PpsI GAGTC 4 cut(s) 1936, 3317, 4486, 4981
Ppu21I YACGTR 2 cut(s) 2416, 3656
PscI ACATGT 2 cut(s) 3730, 4300
PshBI ATTAAT 1 cut(s) 2481
Psp124BI GAGCTC 3 cut(s) 324, 936, 3933
Psp6I CCWGG 4 cut(s) 1163, 1955, 2196, 3723
PspCI CACGTG 1 cut(s) 2416
PspFI CCCAGC 2 cut(s) 3696, 4848
PspGI CCWGG 4 cut(s) 1163, 1955, 2196, 3723
PspN4I GGNNCC 7 cut(s) 423, 776, 1785, 1878, 2525, 4264, 4503
PspPI GGNCC 7 cut(s) 422, 774, 908, 1304, 2524, 2557, 5012
PspXI VCTCGAGB 2 cut(s) 3245, 4475
PstI CTGCAG 2 cut(s) 4219, 4453
PstNI CAGNNNCTG 4 cut(s) 2830, 2873, 3509, 4223
PsuI RGATCY 7 cut(s) 712, 1628, 1864, 1876, 3286, 4036, 4501
PsyI GACNNNGTC 2 cut(s) 2192, 4820
PvuII CAGCTG 5 cut(s) 1747, 3377, 3407, 4220, 4950
RruI TCGCGA 1 cut(s) 172
RseI CAYNNNNRTG 2 cut(s) 3218, 4680
SacI GAGCTC 3 cut(s) 324, 936, 3933
SalI GTCGAC 1 cut(s) 4296
SapI GCTCTTC 2 cut(s) 4002, 5074
Sau96I GGNCC 7 cut(s) 422, 774, 908, 1304, 2524, 2557, 5012
ScaI AGTACT 3 cut(s) 1716, 1770, 4720
SchI GAGTC 4 cut(s) 1937, 3318, 4487, 4981
ScrFI CCNGG 6 cut(s) 420, 772, 1165, 1957, 2198, 3725
SduI GDGCHC 9 cut(s) 324, 936, 1138, 2959, 3578, 3933, 3997, 4621, 5069
SfcI CTRYAG 7 cut(s) 735, 2649, 3226, 3282, 4215, 4246, 4449
Sfr274I CTCGAG 3 cut(s) 3245, 4062, 4475
SfuI TTCGAA 1 cut(s) 2245
SinI GGWCC 5 cut(s) 774, 908, 1304, 2524, 5012
SlaI CTCGAG 3 cut(s) 3245, 4062, 4475
SmiMI CAYNNNNRTG 2 cut(s) 3218, 4680
SmlI CTYRAG 8 cut(s) 2839, 2921, 3245, 4040, 4062, 4389, 4475, 4861
SmoI CTYRAG 8 cut(s) 2839, 2921, 3245, 4040, 4062, 4389, 4475, 4861
SpeI ACTAGT 1 cut(s) 3578
SphI GCATGC 1 cut(s) 2634
SseBI AGGCCT 1 cut(s) 3062
SsiI CCGC 9 cut(s) 126, 520, 2489, 3100, 3184, 4243, 4496, 5073, 5129
SspI AATATT 1 cut(s) 405
SstI GAGCTC 3 cut(s) 324, 936, 3933
StuI AGGCCT 1 cut(s) 3062
StyD4I CCNGG 6 cut(s) 418, 770, 1163, 1955, 2196, 3723
StyI CCWWGG 4 cut(s) 315, 2959, 4910, 5077
TaiI ACGT 5 cut(s) 258, 2418, 3538, 3658, 5066
TaqII GACCGA 1 cut(s) 4156
TatI WGTACW 6 cut(s) 892, 1714, 1768, 2905, 4351, 4718
TauI GCSGC 2 cut(s) 128, 3187
TscAI CASTG 6 cut(s) 969, 1143, 2950, 3235, 4255, 4495
TseFI GTSAC 3 cut(s) 647, 1853, 3439
Tsp45I GTSAC 3 cut(s) 647, 1853, 3439
TspGWI ACGGA 3 cut(s) 1404, 2075, 4171
TspRI CASTG 6 cut(s) 969, 1143, 2950, 3235, 4255, 4495
Tth111I GACNNNGTC 2 cut(s) 2192, 4820
VneI GTGCAC 1 cut(s) 1134
VpaK11BI GGWCC 5 cut(s) 774, 908, 1304, 2524, 5012
VspI ATTAAT 1 cut(s) 2481
XagI CCTNNNNNAGG 1 cut(s) 534
XapI RAATTY 3 cut(s) 427, 667, 923
XceI RCATGY 6 cut(s) 2306, 2634, 3734, 4304, 4652, 4780
XcmI CCANNNNNNNNNTGG 3 cut(s) 3205, 3806, 4495
XhoI CTCGAG 3 cut(s) 3245, 4062, 4475
XmiI GTMKAC 3 cut(s) 1065, 4297, 4382
XmnI GAANNNNTTC 1 cut(s) 405
ZraI GACGTC 1 cut(s) 256
ZrmI AGTACT 3 cut(s) 1716, 1770, 4720
Zsp2I ATGCAT 5 cut(s) 484, 1607, 3691, 4579, 4782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.