MD12G1108500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013519
Physical Location & Seq
Reverse (-)
17155143 .. 17157478
2336 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1108500.v1.1.491

Sequence Viewer

Length: 381 bp
ATGTCGCGCAAAGGAGGAAGTGATTCTTCACTGCCAAAGGACGTGCCTTGGAGGACCTCAAACGTCAAACCCATCCCCAAAATTCACCATTCCCCTCTTCTCCGTATAACCCCCAACCCTACGTCCAATTACGCCCTCTCCGTCATGAAGCACCCGAATCCAGTCGGAAGTGGGTTGGCCATGGAAGCTATAGTGGAATCCGCTGGACCGGATTGCATTGTCCCAGGACAAGTAGCACCCGTCAAATTACTTGGTCTCAAGGTATGGCCTATTGAAGTTAACCTGAAATTTTTGGAACCAGTAGGGCGAGAGCTGAAGCAGCTTGGAAAGTTCATGGATGATGCTGTCAATCTCATGAATAAATCATTTATAGATCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

127

Amino Acids

13.76

Weight (kDa)

9.56

Isoelectric Point (pI)

35.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013519)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36835
fragaria_vesca FvH4_6g16650 FvH4_6g16650 FvH4_6g16650
malus_domestica MD04G1090000.v1.1 MD12G1108500.v1.1
prunus_persica Prupe.7G126900_v2.0.a1
pyrus_communis pycom04g08510
rosa_chinensis RchiOBHm_Chr3g0470611
rosa_laevigata RLG00000024254
rosa_multiflora Rmu_sc0002115.1_g000019
rosa_roxburghii Rroxscaffold_6G00410690
rosa_rugosa Rorug03G0110500
rosa_samantha Rh3AG160600 Rh3BG185100 Rh3CG175600 Rh3DG189600
rosa_wichuraiana Rw3G015180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 7
AciI CCGC 1 cut(s) 201
AcoI YGGCCR 1 cut(s) 177
AcsI RAATTY 2 cut(s) 81, 287
AcuI CTGAAG 1 cut(s) 335
AgsI TTSAA 1 cut(s) 275
AjiI CACGTC 1 cut(s) 43
AjnI CCWGG 1 cut(s) 223
AluBI AGCT 3 cut(s) 188, 313, 322
AluI AGCT 3 cut(s) 188, 313, 322
Alw26I GTCTC 1 cut(s) 260
AoxI GGCC 2 cut(s) 177, 266
ApeKI GCWGC 1 cut(s) 319
ApoI RAATTY 2 cut(s) 81, 287
Asp700I GAANNNNTTC 1 cut(s) 22
AspLEI GCGC 1 cut(s) 9
AspS9I GGNCC 2 cut(s) 54, 206
AsuHPI GGTGA 1 cut(s) 77
AvaII GGWCC 2 cut(s) 54, 206
BalI TGGCCA 1 cut(s) 179
BbvI GCAGC 1 cut(s) 331
BccI CCATC 1 cut(s) 80
BciT130I CCWGG 1 cut(s) 225
BcoDI GTCTC 1 cut(s) 260
BfaI CTAG 1 cut(s) 379
BfmI CTRYAG 1 cut(s) 189
BisI GCNGC 1 cut(s) 320
BlsI GCNGC 1 cut(s) 321
Bme1390I CCNGG 1 cut(s) 225
Bme18I GGWCC 2 cut(s) 54, 206
BmgBI CACGTC 1 cut(s) 43
BmgT120I GGNCC 2 cut(s) 54, 206
BmiI GGNNCC 1 cut(s) 297
BmrFI CCNGG 1 cut(s) 225
BmsI GCATC 1 cut(s) 331
BpuEI CTTGAG 1 cut(s) 242
BsaI GGTCTC 1 cut(s) 260
BsaJI CCNNGG 3 cut(s) 47, 180, 223
BsaWI WCCGGW 1 cut(s) 208
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bse1I ACTGG 2 cut(s) 161, 299
BseBI CCWGG 1 cut(s) 225
BseDI CCNNGG 3 cut(s) 47, 180, 223
BseGI GGATG 2 cut(s) 72, 343
BseNI ACTGG 2 cut(s) 161, 299
BseXI GCAGC 1 cut(s) 331
Bsh1236I CGCG 1 cut(s) 7
BshFI GGCC 2 cut(s) 179, 268
BsiSI CCGG 1 cut(s) 209
BslFI GGGAC 1 cut(s) 206
BsmAI GTCTC 1 cut(s) 260
BsmFI GGGAC 1 cut(s) 206
BsnI GGCC 2 cut(s) 179, 268
Bso31I GGTCTC 1 cut(s) 260
Bsp143I GATC 1 cut(s) 373
Bsp19I CCATGG 1 cut(s) 180
BspACI CCGC 1 cut(s) 201
BspANI GGCC 2 cut(s) 179, 268
BspFNI CGCG 1 cut(s) 7
BspHI TCATGA 2 cut(s) 144, 354
BspLI GGNNCC 1 cut(s) 297
BspTNI GGTCTC 1 cut(s) 260
BsrI ACTGG 2 cut(s) 161, 299
BssECI CCNNGG 3 cut(s) 47, 180, 223
BssMI GATC 1 cut(s) 373
BssT1I CCWWGG 2 cut(s) 47, 180
Bst2UI CCWGG 1 cut(s) 225
Bst6I CTCTTC 1 cut(s) 102
BstDSI CCRYGG 1 cut(s) 180
BstF5I GGATG 2 cut(s) 72, 343
BstFNI CGCG 1 cut(s) 7
BstHHI GCGC 1 cut(s) 9
BstKTI GATC 1 cut(s) 376
BstMAI GTCTC 1 cut(s) 260
BstMBI GATC 1 cut(s) 373
BstMWI GCNNNNNNNGC 2 cut(s) 185, 319
BstNI CCWGG 1 cut(s) 225
BstSCI CCNGG 1 cut(s) 223
BstSFI CTRYAG 1 cut(s) 189
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 1 cut(s) 331
BsuRI GGCC 2 cut(s) 179, 268
BtgI CCRYGG 1 cut(s) 180
BtrI CACGTC 1 cut(s) 43
BtsCI GGATG 2 cut(s) 72, 343
BtsI GCAGTG 1 cut(s) 29
BtsIMutI CAGTG 1 cut(s) 29
CciI TCATGA 2 cut(s) 144, 354
CfoI GCGC 1 cut(s) 9
Cfr13I GGNCC 2 cut(s) 54, 206
CviAII CATG 4 cut(s) 145, 181, 334, 355
CviJI RGCY 5 cut(s) 179, 188, 268, 313, 322
CviKI_1 RGCY 5 cut(s) 179, 188, 268, 313, 322
DpnI GATC 1 cut(s) 375
DpnII GATC 1 cut(s) 373
EaeI YGGCCR 1 cut(s) 177
Eam1104I CTCTTC 1 cut(s) 102
EarI CTCTTC 1 cut(s) 102
Eco130I CCWWGG 2 cut(s) 47, 180
Eco31I GGTCTC 1 cut(s) 260
Eco47I GGWCC 2 cut(s) 54, 206
Eco57I CTGAAG 1 cut(s) 335
EcoO109I RGGNCCY 1 cut(s) 54
EcoRII CCWGG 1 cut(s) 223
EcoT14I CCWWGG 2 cut(s) 47, 180
ErhI CCWWGG 2 cut(s) 47, 180
FaeI CATG 4 cut(s) 148, 184, 337, 358
FaiI YATR 8 cut(s) 107, 146, 182, 191, 265, 335, 356, 371
FalI AAGNNNNNCTT 2 cut(s) 10, 42
FaqI GGGAC 1 cut(s) 206
FatI CATG 4 cut(s) 144, 180, 333, 354
Fnu4HI GCNGC 1 cut(s) 320
FokI GGATG 2 cut(s) 59, 350
Fsp4HI GCNGC 1 cut(s) 320
FspBI CTAG 1 cut(s) 379
GlaI GCGC 1 cut(s) 8
GluI GCNGC 1 cut(s) 320
HaeIII GGCC 2 cut(s) 179, 268
HapII CCGG 1 cut(s) 209
HhaI GCGC 1 cut(s) 9
Hin1II CATG 4 cut(s) 148, 184, 337, 358
Hin6I GCGC 1 cut(s) 7
HinP1I GCGC 1 cut(s) 7
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HinfI GANTC 3 cut(s) 23, 157, 197
HpaI GTTAAC 1 cut(s) 280
HpaII CCGG 1 cut(s) 209
HphI GGTGA 1 cut(s) 77
Hpy166II GTNNAC 1 cut(s) 280
Hpy188I TCNGA 1 cut(s) 167
Hpy188III TCNNGA 2 cut(s) 145, 355
Hpy8I GTNNAC 1 cut(s) 280
HpyCH4IV ACGT 3 cut(s) 42, 63, 122
HpyCH4V TGCA 1 cut(s) 216
HpyF10VI GCNNNNNNNGC 2 cut(s) 185, 319
HpySE526I ACGT 3 cut(s) 42, 63, 122
Hsp92II CATG 4 cut(s) 148, 184, 337, 358
HspAI GCGC 1 cut(s) 7
KspAI GTTAAC 1 cut(s) 280
Kzo9I GATC 1 cut(s) 373
LpnPI CCDG 7 cut(s) 174, 189, 210, 222, 237, 296, 312
Lsp1109I GCAGC 1 cut(s) 331
LweI GCATC 1 cut(s) 331
MaeI CTAG 1 cut(s) 379
MaeII ACGT 3 cut(s) 42, 63, 122
MalI GATC 1 cut(s) 375
MboI GATC 1 cut(s) 373
MboII GAAGA 2 cut(s) 18, 89
MlsI TGGCCA 1 cut(s) 179
MluCI AATT 4 cut(s) 81, 127, 245, 287
MluNI TGGCCA 1 cut(s) 179
MmeI TCCRAC 1 cut(s) 145
MnlI CCTC 5 cut(s) 8, 45, 67, 105, 146
Mox20I TGGCCA 1 cut(s) 179
MroXI GAANNNNTTC 1 cut(s) 22
MscI TGGCCA 1 cut(s) 179
MseI TTAA 1 cut(s) 279
Msp20I TGGCCA 1 cut(s) 179
MspA1I CMGCKG 1 cut(s) 203
MspI CCGG 1 cut(s) 209
MspR9I CCNGG 1 cut(s) 225
MvaI CCWGG 1 cut(s) 225
MvnI CGCG 1 cut(s) 7
MwoI GCNNNNNNNGC 2 cut(s) 185, 319
NcoI CCATGG 1 cut(s) 180
NdeII GATC 1 cut(s) 373
NlaIII CATG 4 cut(s) 148, 184, 337, 358
NlaIV GGNNCC 1 cut(s) 297
PagI TCATGA 2 cut(s) 144, 354
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PdmI GAANNNNTTC 1 cut(s) 22
PfeI GAWTC 3 cut(s) 23, 157, 197
PkrI GCNGC 1 cut(s) 321
PpuMI RGGWCCY 1 cut(s) 54
Psp5II RGGWCCY 1 cut(s) 54
Psp6I CCWGG 1 cut(s) 223
PspGI CCWGG 1 cut(s) 223
PspN4I GGNNCC 1 cut(s) 297
PspPI GGNCC 2 cut(s) 54, 206
PspPPI RGGWCCY 1 cut(s) 54
SaqAI TTAA 1 cut(s) 279
SatI GCNGC 1 cut(s) 320
Sau3AI GATC 1 cut(s) 373
Sau96I GGNCC 2 cut(s) 54, 206
ScrFI CCNGG 1 cut(s) 225
SetI ASST 9 cut(s) 45, 59, 66, 125, 190, 264, 285, 315, 324
SfaNI GCATC 1 cut(s) 331
SfcI CTRYAG 1 cut(s) 189
SinI GGWCC 2 cut(s) 54, 206
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 4 cut(s) 81, 127, 245, 287
SsiI CCGC 1 cut(s) 201
SspMI CTAG 1 cut(s) 379
StyD4I CCNGG 1 cut(s) 223
StyI CCWWGG 2 cut(s) 47, 180
TaiI ACGT 3 cut(s) 45, 66, 125
TasI AATT 4 cut(s) 81, 127, 245, 287
TfiI GAWTC 3 cut(s) 23, 157, 197
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TscAI CASTG 1 cut(s) 36
TseI GCWGC 1 cut(s) 319
TspDTI ATGAA 3 cut(s) 161, 322, 371
TspGWI ACGGA 2 cut(s) 92, 130
TspRI CASTG 1 cut(s) 36
VpaK11BI GGWCC 2 cut(s) 54, 206
XapI RAATTY 2 cut(s) 81, 287
XmnI GAANNNNTTC 1 cut(s) 22
XspI CTAG 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.