RLG00000024254

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013519
Physical Location & Seq
Reverse (-)
33599753 .. 33601562
1810 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024254

Sequence Viewer

Length: 384 bp
ATGTCCCGCAAAGGAGGAGGAGCTCCGGCTTCACTGCCAAAGGACGTACCTTGGAGGGCCTCAAACTCCAGACCCGTCCCCAAAATCCACCACTCCCCTATTCTTCGTGTGCCCCAAAACCCCACTACCAATTATGCAATCTCCGTCATGAAGCACCCCAATCCGGTAGGAAATGGGTTGGCCAGGGACGCCATTGTGGAAGCGGCAGGACCCGATTGCATTGTTCCGGGTCAAGTCACACCCGTCAAATTACTTGGTCTCAAGGTATGGCCTATTGAAGTTGACCTACAATTTTTGGAACCCATTGGACGTGAGCTTAAGTCTATTGGAAAGTTCATGGATGATGCTGTCACTCTCATGAATAAATCATTTATAGATCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

128

Amino Acids

13.78

Weight (kDa)

9.66

Isoelectric Point (pI)

38.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013519)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36835
fragaria_vesca FvH4_6g16650 FvH4_6g16650 FvH4_6g16650
malus_domestica MD04G1090000.v1.1 MD12G1108500.v1.1
prunus_persica Prupe.7G126900_v2.0.a1
pyrus_communis pycom04g08510
rosa_chinensis RchiOBHm_Chr3g0470611
rosa_laevigata RLG00000024254
rosa_multiflora Rmu_sc0002115.1_g000019
rosa_roxburghii Rroxscaffold_6G00410690
rosa_rugosa Rorug03G0110500
rosa_samantha Rh3AG160600 Rh3BG185100 Rh3CG175600 Rh3DG189600
rosa_wichuraiana Rw3G015180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 7, 203
AcoI YGGCCR 1 cut(s) 180
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 1 cut(s) 48
AfiI CCNNNNNNNGG 1 cut(s) 163
AflII CTTAAG 1 cut(s) 317
AgsI TTSAA 1 cut(s) 278
AjiI CACGTC 1 cut(s) 311
AjnI CCWGG 1 cut(s) 182
AluBI AGCT 2 cut(s) 23, 316
AluI AGCT 2 cut(s) 23, 316
Alw21I GWGCWC 1 cut(s) 25
Alw26I GTCTC 1 cut(s) 263
AoxI GGCC 3 cut(s) 57, 180, 269
AspS9I GGNCC 2 cut(s) 57, 209
AsuC2I CCSGG 1 cut(s) 228
AvaII GGWCC 1 cut(s) 209
BaeGI GKGCMC 1 cut(s) 114
BalI TGGCCA 1 cut(s) 182
BanII GRGCYC 1 cut(s) 25
BarI GAAGNNNNNNTAC 2 cut(s) 270, 302
Bbv12I GWGCWC 1 cut(s) 25
BciT130I CCWGG 1 cut(s) 184
BcnI CCSGG 1 cut(s) 228
BcoDI GTCTC 1 cut(s) 263
BfaI CTAG 1 cut(s) 382
BfrI CTTAAG 1 cut(s) 317
BisI GCNGC 1 cut(s) 204
BlsI GCNGC 1 cut(s) 205
Bme1390I CCNGG 2 cut(s) 184, 228
Bme18I GGWCC 1 cut(s) 209
BmgBI CACGTC 1 cut(s) 311
BmgT120I GGNCC 2 cut(s) 57, 209
BmiI GGNNCC 2 cut(s) 211, 300
BmrFI CCNGG 2 cut(s) 184, 228
BmsI GCATC 1 cut(s) 334
BpmI CTGGAG 1 cut(s) 52
BpuEI CTTGAG 1 cut(s) 245
BpuMI CCSGG 1 cut(s) 228
BsaHI GRCGYC 1 cut(s) 189
BsaI GGTCTC 1 cut(s) 263
BsaJI CCNNGG 2 cut(s) 50, 183
BsaWI WCCGGW 1 cut(s) 163
Bsc4I CCNNNNNNNGG 1 cut(s) 163
BseBI CCWGG 1 cut(s) 184
BseDI CCNNGG 2 cut(s) 50, 183
BseGI GGATG 1 cut(s) 346
BseLI CCNNNNNNNGG 1 cut(s) 163
BseRI GAGGAG 2 cut(s) 30, 33
BseSI GKGCMC 1 cut(s) 114
BshFI GGCC 3 cut(s) 59, 182, 271
BsiHKAI GWGCWC 1 cut(s) 25
BsiSI CCGG 3 cut(s) 26, 164, 227
BslFI GGGAC 2 cut(s) 62, 200
BslI CCNNNNNNNGG 1 cut(s) 163
BsmAI GTCTC 1 cut(s) 263
BsmFI GGGAC 2 cut(s) 62, 200
BsnI GGCC 3 cut(s) 59, 182, 271
Bso31I GGTCTC 1 cut(s) 263
Bsp1286I GDGCHC 2 cut(s) 25, 114
Bsp143I GATC 1 cut(s) 376
BspACI CCGC 2 cut(s) 7, 203
BspANI GGCC 3 cut(s) 59, 182, 271
BspHI TCATGA 2 cut(s) 147, 357
BspLI GGNNCC 2 cut(s) 211, 300
BspTI CTTAAG 1 cut(s) 317
BspTNI GGTCTC 1 cut(s) 263
BssECI CCNNGG 2 cut(s) 50, 183
BssMI GATC 1 cut(s) 376
BssNI GRCGYC 1 cut(s) 189
BssT1I CCWWGG 1 cut(s) 50
Bst2UI CCWGG 1 cut(s) 184
BstACI GRCGYC 1 cut(s) 189
BstAFI CTTAAG 1 cut(s) 317
BstF5I GGATG 1 cut(s) 346
BstKTI GATC 1 cut(s) 379
BstMAI GTCTC 1 cut(s) 263
BstMBI GATC 1 cut(s) 376
BstMWI GCNNNNNNNGC 1 cut(s) 188
BstNI CCWGG 1 cut(s) 184
BstSCI CCNGG 2 cut(s) 182, 226
BstSLI GKGCMC 1 cut(s) 114
BsuRI GGCC 3 cut(s) 59, 182, 271
BtrI CACGTC 1 cut(s) 311
BtsCI GGATG 1 cut(s) 346
BtsI GCAGTG 1 cut(s) 32
BtsIMutI CAGTG 1 cut(s) 32
CciI TCATGA 2 cut(s) 147, 357
Cfr13I GGNCC 2 cut(s) 57, 209
CseI GACGC 1 cut(s) 197
Csp6I GTAC 1 cut(s) 47
CviAII CATG 3 cut(s) 148, 337, 358
CviJI RGCY 6 cut(s) 23, 29, 59, 182, 271, 316
CviKI_1 RGCY 6 cut(s) 23, 29, 59, 182, 271, 316
CviQI GTAC 1 cut(s) 47
DpnI GATC 1 cut(s) 378
DpnII GATC 1 cut(s) 376
EaeI YGGCCR 1 cut(s) 180
Ecl136II GAGCTC 1 cut(s) 23
Eco130I CCWWGG 1 cut(s) 50
Eco24I GRGCYC 1 cut(s) 25
Eco31I GGTCTC 1 cut(s) 263
Eco47I GGWCC 1 cut(s) 209
Eco53kI GAGCTC 1 cut(s) 23
EcoICRI GAGCTC 1 cut(s) 23
EcoO109I RGGNCCY 2 cut(s) 57, 209
EcoRII CCWGG 1 cut(s) 182
EcoT14I CCWWGG 1 cut(s) 50
EcoT38I GRGCYC 1 cut(s) 25
ErhI CCWWGG 1 cut(s) 50
FaeI CATG 3 cut(s) 151, 340, 361
FaiI YATR 6 cut(s) 135, 149, 268, 338, 359, 374
FaqI GGGAC 2 cut(s) 62, 200
FatI CATG 3 cut(s) 147, 336, 357
FauI CCCGC 1 cut(s) 14
Fnu4HI GCNGC 1 cut(s) 204
FokI GGATG 1 cut(s) 353
FriOI GRGCYC 1 cut(s) 25
Fsp4HI GCNGC 1 cut(s) 204
FspBI CTAG 1 cut(s) 382
GluI GCNGC 1 cut(s) 204
GsuI CTGGAG 1 cut(s) 52
HaeIII GGCC 3 cut(s) 59, 182, 271
HapII CCGG 3 cut(s) 26, 164, 227
HgaI GACGC 1 cut(s) 197
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 3 cut(s) 151, 340, 361
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HpaII CCGG 3 cut(s) 26, 164, 227
Hpy166II GTNNAC 1 cut(s) 283
Hpy188III TCNNGA 3 cut(s) 69, 148, 358
Hpy8I GTNNAC 1 cut(s) 283
HpyCH4IV ACGT 2 cut(s) 45, 310
HpyCH4V TGCA 2 cut(s) 137, 219
HpyF10VI GCNNNNNNNGC 1 cut(s) 188
HpySE526I ACGT 2 cut(s) 45, 310
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 3 cut(s) 151, 340, 361
Kzo9I GATC 1 cut(s) 376
LmnI GCTCC 2 cut(s) 20, 28
LpnPI CCDG 7 cut(s) 39, 82, 169, 177, 192, 196, 240
LweI GCATC 1 cut(s) 334
MaeI CTAG 1 cut(s) 382
MaeII ACGT 2 cut(s) 45, 310
MaeIII GTNAC 2 cut(s) 235, 349
MalI GATC 1 cut(s) 378
MboI GATC 1 cut(s) 376
MboII GAAGA 1 cut(s) 95
MhlI GDGCHC 2 cut(s) 25, 114
MlsI TGGCCA 1 cut(s) 182
MluCI AATT 3 cut(s) 130, 248, 290
MluNI TGGCCA 1 cut(s) 182
MnlI CCTC 4 cut(s) 8, 11, 48, 70
Mox20I TGGCCA 1 cut(s) 182
MscI TGGCCA 1 cut(s) 182
MseI TTAA 1 cut(s) 318
MslI CAYNNNNRTG 1 cut(s) 356
Msp20I TGGCCA 1 cut(s) 182
MspCI CTTAAG 1 cut(s) 317
MspI CCGG 3 cut(s) 26, 164, 227
MspR9I CCNGG 2 cut(s) 184, 228
MvaI CCWGG 1 cut(s) 184
MwoI GCNNNNNNNGC 1 cut(s) 188
NciI CCSGG 1 cut(s) 228
NdeII GATC 1 cut(s) 376
NlaIII CATG 3 cut(s) 151, 340, 361
NlaIV GGNNCC 2 cut(s) 211, 300
NmuCI GTSAC 2 cut(s) 235, 349
PagI TCATGA 2 cut(s) 147, 357
PkrI GCNGC 1 cut(s) 205
PpuMI RGGWCCY 1 cut(s) 209
Psp124BI GAGCTC 1 cut(s) 25
Psp5II RGGWCCY 1 cut(s) 209
Psp6I CCWGG 1 cut(s) 182
PspGI CCWGG 1 cut(s) 182
PspN4I GGNNCC 2 cut(s) 211, 300
PspPI GGNCC 2 cut(s) 57, 209
PspPPI RGGWCCY 1 cut(s) 209
RsaI GTAC 1 cut(s) 48
RsaNI GTAC 1 cut(s) 47
RseI CAYNNNNRTG 1 cut(s) 356
SacI GAGCTC 1 cut(s) 25
SaqAI TTAA 1 cut(s) 318
SatI GCNGC 1 cut(s) 204
Sau3AI GATC 1 cut(s) 376
Sau96I GGNCC 2 cut(s) 57, 209
ScrFI CCNGG 2 cut(s) 184, 228
SduI GDGCHC 2 cut(s) 25, 114
SetI ASST 7 cut(s) 25, 48, 52, 267, 288, 313, 318
SfaNI GCATC 1 cut(s) 334
SinI GGWCC 1 cut(s) 209
SmiMI CAYNNNNRTG 1 cut(s) 356
SmlI CTYRAG 2 cut(s) 260, 317
SmoI CTYRAG 2 cut(s) 260, 317
Sse9I AATT 3 cut(s) 130, 248, 290
SsiI CCGC 2 cut(s) 7, 203
SspMI CTAG 1 cut(s) 382
SstI GAGCTC 1 cut(s) 25
StyD4I CCNGG 2 cut(s) 182, 226
StyI CCWWGG 1 cut(s) 50
TaiI ACGT 2 cut(s) 48, 313
TasI AATT 3 cut(s) 130, 248, 290
TauI GCSGC 1 cut(s) 206
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TscAI CASTG 1 cut(s) 39
TseFI GTSAC 2 cut(s) 235, 349
Tsp45I GTSAC 2 cut(s) 235, 349
TspDTI ATGAA 3 cut(s) 164, 325, 374
TspGWI ACGGA 1 cut(s) 133
TspRI CASTG 1 cut(s) 39
Vha464I CTTAAG 1 cut(s) 317
VpaK11BI GGWCC 1 cut(s) 209
XspI CTAG 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.