pycom04g08510

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0013519
Physical Location & Seq
Reverse (-)
10197269 .. 10199726
2458 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g08510.2

Sequence Viewer

Length: 537 bp
ATGACAAACGCCAAAAGGAAATACAAAACCTCCAGCGGCTTTGCTCCTCATCATCTTCATCATCCACCTCAAAAATCCACCCAGCTTTGCTCACCTCTCCCTCTCCCTTTCCCTCTCCCTGCCTCTCTACAGTCAGTTTACGTACGAAAGAAGGACATGTCCCGCAAAGGAGGAGGAAGTGCTTCTTCACTCCCAAAGGACGTGCCTTGGAGGGCCTCAAACATCAAACCCATCCCCAAAATTCACCATTCCCCTCTTCTCCGCATAGCCCCCAACCCTACTTCCAACTATGCCCTCTCCGTCATGAAGCACCCGAATCCAGTCGGAAGTGGGTTGGCCATGGAAGCTATAGTGGAATCCGCCGGACCGGATTGCATTGTCCCTGGACAAGTTGCACCCGTCAAATTACTTGGTCTCAAGGTATGGCCTATTGAAGTTAACTTCAAATTTTTGGAACCAGTTGAGCGAGAGCTCAAGCAGCTTGGAAGGTTCATGGATGATGCTGTCAATCTCATGAATAAATCCTTTTCCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

179

Amino Acids

19.53

Weight (kDa)

9.97

Isoelectric Point (pI)

48.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013519)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36835
fragaria_vesca FvH4_6g16650 FvH4_6g16650 FvH4_6g16650
malus_domestica MD04G1090000.v1.1 MD12G1108500.v1.1
prunus_persica Prupe.7G126900_v2.0.a1
pyrus_communis pycom04g08510
rosa_chinensis RchiOBHm_Chr3g0470611
rosa_laevigata RLG00000024254
rosa_multiflora Rmu_sc0002115.1_g000019
rosa_roxburghii Rroxscaffold_6G00410690
rosa_rugosa Rorug03G0110500
rosa_samantha Rh3AG160600 Rh3BG185100 Rh3CG175600 Rh3DG189600
rosa_wichuraiana Rw3G015180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 36, 163, 262, 360
AcoI YGGCCR 1 cut(s) 336
AcsI RAATTY 2 cut(s) 240, 446
AfaI GTAC 1 cut(s) 144
AflIII ACRYGT 1 cut(s) 156
AgsI TTSAA 2 cut(s) 434, 445
AjiI CACGTC 1 cut(s) 202
AjnI CCWGG 1 cut(s) 382
AluBI AGCT 4 cut(s) 85, 347, 472, 481
AluI AGCT 4 cut(s) 85, 347, 472, 481
Alw21I GWGCWC 1 cut(s) 474
Alw26I GTCTC 1 cut(s) 419
AoxI GGCC 3 cut(s) 213, 336, 425
ApeKI GCWGC 1 cut(s) 478
ApoI RAATTY 2 cut(s) 240, 446
Asp700I GAANNNNTTC 1 cut(s) 181
AspS9I GGNCC 2 cut(s) 213, 365
AsuHPI GGTGA 2 cut(s) 84, 236
AvaII GGWCC 1 cut(s) 365
BalI TGGCCA 1 cut(s) 338
BanII GRGCYC 1 cut(s) 474
Bbv12I GWGCWC 1 cut(s) 474
BbvI GCAGC 1 cut(s) 490
BccI CCATC 1 cut(s) 239
BciT130I CCWGG 1 cut(s) 384
BcoDI GTCTC 1 cut(s) 419
BfmI CTRYAG 2 cut(s) 128, 348
BisI GCNGC 2 cut(s) 37, 479
BlsI GCNGC 2 cut(s) 38, 480
Bme1390I CCNGG 1 cut(s) 384
Bme18I GGWCC 1 cut(s) 365
BmgBI CACGTC 1 cut(s) 202
BmgT120I GGNCC 2 cut(s) 213, 365
BmiI GGNNCC 1 cut(s) 456
BmrFI CCNGG 1 cut(s) 384
BmsI GCATC 1 cut(s) 490
BpmI CTGGAG 1 cut(s) 16
BpuEI CTTGAG 2 cut(s) 401, 458
BsaAI YACGTR 1 cut(s) 142
BsaI GGTCTC 1 cut(s) 419
BsaJI CCNNGG 3 cut(s) 206, 339, 382
BsaWI WCCGGW 1 cut(s) 367
BsaXI ACNNNNNCTCC 2 cut(s) 14, 44
Bse1I ACTGG 2 cut(s) 320, 458
BseBI CCWGG 1 cut(s) 384
BseDI CCNNGG 3 cut(s) 206, 339, 382
BseGI GGATG 3 cut(s) 61, 231, 502
BseNI ACTGG 2 cut(s) 320, 458
BseRI GAGGAG 2 cut(s) 36, 186
BseXI GCAGC 1 cut(s) 490
BseYI CCCAGC 1 cut(s) 81
BshFI GGCC 3 cut(s) 215, 338, 427
BsiHKAI GWGCWC 1 cut(s) 474
BsiSI CCGG 2 cut(s) 363, 368
BsiWI CGTACG 1 cut(s) 142
BslFI GGGAC 2 cut(s) 145, 365
BsmAI GTCTC 1 cut(s) 419
BsmFI GGGAC 2 cut(s) 145, 365
BsnI GGCC 3 cut(s) 215, 338, 427
Bso31I GGTCTC 1 cut(s) 419
Bsp1286I GDGCHC 1 cut(s) 474
Bsp19I CCATGG 1 cut(s) 339
BspACI CCGC 4 cut(s) 36, 163, 262, 360
BspANI GGCC 3 cut(s) 215, 338, 427
BspHI TCATGA 2 cut(s) 303, 513
BspLI GGNNCC 1 cut(s) 456
BspTNI GGTCTC 1 cut(s) 419
BsrI ACTGG 2 cut(s) 320, 458
BssECI CCNNGG 3 cut(s) 206, 339, 382
BssT1I CCWWGG 2 cut(s) 206, 339
Bst2UI CCWGG 1 cut(s) 384
Bst4CI ACNGT 1 cut(s) 132
Bst6I CTCTTC 1 cut(s) 261
BstBAI YACGTR 1 cut(s) 142
BstDSI CCRYGG 1 cut(s) 339
BstF5I GGATG 3 cut(s) 61, 231, 502
BstMAI GTCTC 1 cut(s) 419
BstMWI GCNNNNNNNGC 2 cut(s) 344, 478
BstNI CCWGG 1 cut(s) 384
BstNSI RCATGY 1 cut(s) 160
BstSCI CCNGG 1 cut(s) 382
BstSFI CTRYAG 2 cut(s) 128, 348
BstSNI TACGTA 1 cut(s) 142
BstV1I GCAGC 1 cut(s) 490
BsuRI GGCC 3 cut(s) 215, 338, 427
BtgI CCRYGG 1 cut(s) 339
BtrI CACGTC 1 cut(s) 202
BtsCI GGATG 3 cut(s) 61, 231, 502
CciI TCATGA 2 cut(s) 303, 513
Cfr13I GGNCC 2 cut(s) 213, 365
CpoI CGGWCCG 1 cut(s) 365
Csp6I GTAC 1 cut(s) 143
CspI CGGWCCG 1 cut(s) 365
CviAII CATG 5 cut(s) 157, 304, 340, 493, 514
CviJI RGCY 9 cut(s) 39, 85, 215, 269, 338, 347, 427, 472, 481
CviKI_1 RGCY 9 cut(s) 39, 85, 215, 269, 338, 347, 427, 472, 481
CviQI GTAC 1 cut(s) 143
EaeI YGGCCR 1 cut(s) 336
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
EciI GGCGGA 1 cut(s) 349
Ecl136II GAGCTC 1 cut(s) 472
Eco105I TACGTA 1 cut(s) 142
Eco130I CCWWGG 2 cut(s) 206, 339
Eco24I GRGCYC 1 cut(s) 474
Eco31I GGTCTC 1 cut(s) 419
Eco47I GGWCC 1 cut(s) 365
Eco53kI GAGCTC 1 cut(s) 472
EcoICRI GAGCTC 1 cut(s) 472
EcoO109I RGGNCCY 1 cut(s) 213
EcoRII CCWGG 1 cut(s) 382
EcoT14I CCWWGG 2 cut(s) 206, 339
EcoT38I GRGCYC 1 cut(s) 474
ErhI CCWWGG 2 cut(s) 206, 339
FaeI CATG 5 cut(s) 160, 307, 343, 496, 517
FaiI YATR 9 cut(s) 158, 266, 291, 305, 341, 350, 424, 494, 515
FalI AAGNNNNNCTT 2 cut(s) 169, 201
FaqI GGGAC 2 cut(s) 145, 365
FatI CATG 5 cut(s) 156, 303, 339, 492, 513
FauI CCCGC 1 cut(s) 170
Fnu4HI GCNGC 2 cut(s) 37, 479
FokI GGATG 3 cut(s) 48, 218, 509
FriOI GRGCYC 1 cut(s) 474
Fsp4HI GCNGC 2 cut(s) 37, 479
GluI GCNGC 2 cut(s) 37, 479
GsaI CCCAGC 1 cut(s) 85
GsuI CTGGAG 1 cut(s) 16
HaeIII GGCC 3 cut(s) 215, 338, 427
HapII CCGG 2 cut(s) 363, 368
Hin1II CATG 5 cut(s) 160, 307, 343, 496, 517
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HinfI GANTC 2 cut(s) 316, 356
HpaI GTTAAC 1 cut(s) 439
HpaII CCGG 2 cut(s) 363, 368
HphI GGTGA 2 cut(s) 84, 236
Hpy166II GTNNAC 2 cut(s) 139, 439
Hpy188I TCNGA 2 cut(s) 326, 536
Hpy188III TCNNGA 2 cut(s) 304, 514
Hpy8I GTNNAC 2 cut(s) 139, 439
HpyAV CCTTC 2 cut(s) 145, 480
HpyCH4III ACNGT 1 cut(s) 132
HpyCH4IV ACGT 2 cut(s) 141, 201
HpyCH4V TGCA 2 cut(s) 375, 395
HpyF10VI GCNNNNNNNGC 2 cut(s) 344, 478
HpySE526I ACGT 2 cut(s) 141, 201
Hsp92II CATG 5 cut(s) 160, 307, 343, 496, 517
KspAI GTTAAC 1 cut(s) 439
LmnI GCTCC 1 cut(s) 49
LpnPI CCDG 9 cut(s) 46, 95, 132, 333, 369, 376, 381, 396, 471
Lsp1109I GCAGC 1 cut(s) 490
LweI GCATC 1 cut(s) 490
MaeII ACGT 2 cut(s) 141, 201
MboII GAAGA 3 cut(s) 47, 177, 248
MhlI GDGCHC 1 cut(s) 474
MlsI TGGCCA 1 cut(s) 338
MluCI AATT 3 cut(s) 240, 404, 446
MluNI TGGCCA 1 cut(s) 338
MmeI TCCRAC 2 cut(s) 304, 309
Mox20I TGGCCA 1 cut(s) 338
MroXI GAANNNNTTC 1 cut(s) 181
MscI TGGCCA 1 cut(s) 338
MseI TTAA 1 cut(s) 438
Msp20I TGGCCA 1 cut(s) 338
MspA1I CMGCKG 1 cut(s) 36
MspI CCGG 2 cut(s) 363, 368
MspR9I CCNGG 1 cut(s) 384
MvaI CCWGG 1 cut(s) 384
MwoI GCNNNNNNNGC 2 cut(s) 344, 478
NcoI CCATGG 1 cut(s) 339
NlaIII CATG 5 cut(s) 160, 307, 343, 496, 517
NlaIV GGNNCC 1 cut(s) 456
NspI RCATGY 1 cut(s) 160
PagI TCATGA 2 cut(s) 303, 513
PciI ACATGT 1 cut(s) 156
PdmI GAANNNNTTC 1 cut(s) 181
PfeI GAWTC 2 cut(s) 316, 356
Pfl23II CGTACG 1 cut(s) 142
PkrI GCNGC 2 cut(s) 38, 480
Ppu21I YACGTR 1 cut(s) 142
PscI ACATGT 1 cut(s) 156
Psp124BI GAGCTC 1 cut(s) 474
Psp6I CCWGG 1 cut(s) 382
PspFI CCCAGC 1 cut(s) 81
PspGI CCWGG 1 cut(s) 382
PspLI CGTACG 1 cut(s) 142
PspN4I GGNNCC 1 cut(s) 456
PspPI GGNCC 2 cut(s) 213, 365
RsaI GTAC 1 cut(s) 144
RsaNI GTAC 1 cut(s) 143
Rsr2I CGGWCCG 1 cut(s) 365
RsrII CGGWCCG 1 cut(s) 365
SacI GAGCTC 1 cut(s) 474
SaqAI TTAA 1 cut(s) 438
SatI GCNGC 2 cut(s) 37, 479
Sau96I GGNCC 2 cut(s) 213, 365
ScrFI CCNGG 1 cut(s) 384
SduI GDGCHC 1 cut(s) 474
SfaNI GCATC 1 cut(s) 490
SfcI CTRYAG 2 cut(s) 128, 348
SinI GGWCC 1 cut(s) 365
SmlI CTYRAG 2 cut(s) 416, 473
SmoI CTYRAG 2 cut(s) 416, 473
SnaBI TACGTA 1 cut(s) 142
Sse9I AATT 3 cut(s) 240, 404, 446
SsiI CCGC 4 cut(s) 36, 163, 262, 360
SstI GAGCTC 1 cut(s) 474
StyD4I CCNGG 1 cut(s) 382
StyI CCWWGG 2 cut(s) 206, 339
TaaI ACNGT 1 cut(s) 132
TaiI ACGT 2 cut(s) 144, 204
TasI AATT 3 cut(s) 240, 404, 446
TauI GCSGC 1 cut(s) 39
TfiI GAWTC 2 cut(s) 316, 356
Tru1I TTAA 1 cut(s) 438
Tru9I TTAA 1 cut(s) 438
TseI GCWGC 1 cut(s) 478
TspDTI ATGAA 4 cut(s) 47, 320, 481, 530
TspGWI ACGGA 1 cut(s) 289
VpaK11BI GGWCC 1 cut(s) 365
XapI RAATTY 2 cut(s) 240, 446
XceI RCATGY 1 cut(s) 160
XmnI GAANNNNTTC 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.