Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0014545

Physical Location & Seq

Genomic Coordinates Reverse (-)

19757682 .. 19759958

Gene Structure 2277 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1123300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2277 bp

ATGAAAAGATTAAAATCCCCCCTACCGTTGACATTCCACAAGAGTCAACCAAGTCGACCTCCATCTCTTTTACCAAACTTCAACAACGAACAGTTTAGCAACAACTACATAATCTCCTTGTGCAAGCAAAAGCTTTACAGAGAAGCCATTGAAGCATTTGAGTTTTTACAAGGTCACACGAACTTCCCAATATTTCCCCGCACTTACACAAATTTGGTTTATGCTTGCTCGTCTTTGAGGTCTCTCGAACACGGCCGGAGAATCCATGGCCATACTTTGGCATCCAATTACCAGCCGGACATTGTTTTTCAGAATCATGTTCTCAACATGTATGGAAAATGTGGGTCGCTCGCGGATGCACGTAAGGTTTTCGATGCAATGCCTGAGAGAAATGTGGTTTCTTGGACTTCACTGATTTCTGGGTACTCTCAGAATAGTCAAGAGGATAAAGCCATTGAATTGTATTTCCAAATGCTGAGGGCAGGTTGTGTGCCTGATCACTTCACCTTTGGAAGCGTCCTAAAAGCTTGTTCGGGTTTGCGGAATGAACTGCTGGGGAGACAGCTGCATGCCCATGTTGTTAAATCAGAAACTGGTTCTCATCCTATAGCACAGAATGCCCTTACTTCAATGTACACGAAGTTCGGTCTGATTGCAGATGCTTTTGATGTCTTTTCTCGTGTTCCGACAAAGGACTTGATTTCTTGGGGTTCGATGATTGCAGGGTTTTCCCAGCTTGGTTATGAAAAAGAAGCTTTGGATCACTTCAAAGAAATGCTCTTTCAGGGTGCTTACCAGCCGAATGAGTTTATATTTGGGAGTGCTTTCAGTGCGTGTGGCGGCCTTCTTGAACCTGAATATGGAAAGCAGATACATGGGATGTGCATAAAATTTGGTTTGGGGAGAGACATCTTTGCTGGATGCTCCCTTTGTGACATGTATGCAAAATGTGGGCAATTGGAATCTGCAAGAACAGTGTTTTATCAGATAGACAGGCCCGATTTAGTGTCCTGGAATGCAATTATTTCTGGGTTTTCGAATGCTGGTGATTCTAATGAAGCATTATTATTCTTTTCTCAGATGCGGCATAAGGGACTGATCCCTGACGAGGTTAGTGTTCTCCCCATACTAACTGCTTGCACAAGCCCTTCAACACTCTATCAGGGTGAGCAGATCCACTCCTACATTATCAAGAGGGCTTTTGATTTTACCGTTATTGTATGCAACAGTTTGCTAACCATGTATGCCAAGTGTTCAAATCTCTATGATGCATTTCTTGTGTTTGAAGATATAAGAAATGATGCTGATTTAGTTACTTGGAATGCTATTATTAGCGCGTGCATGCAGCACAACCAGGCTGGGGAGGTTTTCAGATTACTAAATTTAATGCGTATTTCTGAAACTACACCTGATCATATTACTTTGGGTAATTTGATTGGAGCATGTGCAAATATATCATCTCTAGAGGTTGGGAATCAGATTCATTGCTTTACTGTAAAAAGTGGGATTGTGCTTGATGTTACTGTCAGCAATGGATTAATTGACATGTATACAAAGTGTGGATCAATTGGAAGTGCGCAAAAGCTCTTCGGCTGGATGGAGGATCCAGATGTTGTCTCGTGGAGTAGTTTAATCGTGGGTTATGCTCAGTTTGGATATGGAGAGGAAGCTCTTGACCTTTTCAAAAGAATGACGGAATTGGGCATAAAACCCAATGAAGTTACACTGGTGGGTGTCCTTACTGCTTGCAGTCATATTGGACTGGTAGAAGAAGGGTGGAAAATATATAAAACCATGGAATCCGAGCATGGGATTGTACCAACAATAGAGCATTGTTCTTGTATGGTTGACTTACTTGCTCGTTCCGGATGCTTACATGAAGCAGAGGCATTCATCACGCAAATGGCATTTGAACCTGACGTTCTGGTATGGATGACTCTACTTGCTGCCTGCAGAACCCGTGGGAACGTTGAGATTGGAAAACGGGCAGCAGAGAATATACTAAAATTAGATCCTTCCCATTCTGCTGCTCTCGTGCTGCTTTGCAACATCCATGCTTCTTCTGGCAATTGGGATGATGTTGCCAGGTTGAGGAACTTGATGAGAGAAAGGGACGTCAGGAAAGTCCCAGGGCAGAGCTGGATTGAGGTTAAGGATAGGATCCATGTATTTTTTGTTGAAGATTGCCTGCATCCTGAGAGAGGTAAAATATATGCAATGCTAGAGGAGTTGTGGCTGCAGATGCTGGATGATGGTTATGACCCACTTCAGGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

759

Amino Acids

84.81

Weight (kDa)

5.83

Isoelectric Point (pI)

42.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	130 - 177	1.3e-11	PPR repeat family
PPR	PF01535	133 - 163	2.2e-07	PPR repeat
PPR_2	PF13041	333 - 373	3.5e-09	PPR repeat family
PPR	PF01535	336 - 366	3.5e-06	PPR repeat
PPR_2	PF13041	436 - 484	1.6e-08	PPR repeat family
PPR_2	PF13041	536 - 584	1.9e-10	PPR repeat family
PPR	PF01535	539 - 569	6.5e-08	PPR repeat
E_motif	PF20431	656 - 716	4.8e-17	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014545)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53360 AT3G53360
fragaria_vesca	FvH4_6g15460
malus_domestica	MD04G1103100.v1.1 MD12G1123300.v1.1
prunus_persica	Prupe.6G239900_v2.0.a1
pyrus_communis	pycom04g09820 pycom12g12200
rosa_chinensis	RchiOBHm_Chr3g0468941
rosa_laevigata	RLG00000024398
rosa_multiflora	Rmu_co8434945.1_g000001
rosa_roxburghii	Rroxscaffold_6G00412440
rosa_rugosa	Rorug03G0097700
rosa_samantha	Rh3AG147500 Rh3BG170100 Rh3DG205100
rosa_wichuraiana	Rw3G013870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	2118
Acc16I	TGCGCA	1 cut(s)	1578
Acc36I	ACCTGC	1 cut(s)	473
AccB7I	CCANNNNNTGG	1 cut(s)	277
AccI	GTMKAC	2 cut(s)	55, 1550
AccII	CGCG	2 cut(s)	353, 1337
AccIII	TCCGGA	1 cut(s)	1865
AciI	CCGC	5 cut(s)	199, 353, 541, 840, 1084
AclI	AACGTT	1 cut(s)	1968
AclWI	GGATC	9 cut(s)	768, 1093, 1168, 1570, 1598, 1611, 2006, 2155, 2168
AcoI	YGGCCR	2 cut(s)	253, 268
AcsI	RAATTY	3 cut(s)	211, 890, 1381
AcuI	CTGAAG	1 cut(s)	2252
AcyI	GRCGYC	1 cut(s)	2115
AfaI	GTAC	3 cut(s)	425, 635, 1818
AfiI	CCNNNNNNNGG	8 cut(s)	277, 860, 1108, 1360, 1809, 2091, 2201, 2269
AflIII	ACRYGT	3 cut(s)	327, 936, 1545
AjnI	CCWGG	4 cut(s)	1010, 1353, 2084, 2128
AluBI	AGCT	8 cut(s)	133, 527, 565, 736, 755, 1585, 1670, 2139
AluI	AGCT	8 cut(s)	133, 527, 565, 736, 755, 1585, 1670, 2139
Alw26I	GTCTC	4 cut(s)	246, 553, 900, 1621
AlwI	GGATC	9 cut(s)	768, 1093, 1168, 1570, 1598, 1611, 2006, 2155, 2168
AlwNI	CAGNNNCTG	2 cut(s)	593, 2245
Aor13HI	TCCGGA	1 cut(s)	1865
AoxI	GGCC	4 cut(s)	253, 268, 841, 995
ApeKI	GCWGC	7 cut(s)	565, 1345, 1946, 1988, 2027, 2038, 2236
ApoI	RAATTY	3 cut(s)	211, 890, 1381
AseI	ATTAAT	1 cut(s)	1538
AspLEI	GCGC	2 cut(s)	1337, 1579
AspS9I	GGNCC	1 cut(s)	996
AsuHPI	GGTGA	3 cut(s)	496, 1058, 1178
AsuII	TTCGAA	1 cut(s)	1037
BalI	TGGCCA	1 cut(s)	270
BamHI	GGATCC	2 cut(s)	1603, 2160
BauI	CACGAG	3 cut(s)	678, 1618, 2033
BbvCI	CCTCAGC	1 cut(s)	476
BbvI	GCAGC	7 cut(s)	552, 1357, 1933, 2000, 2014, 2025, 2223
BccI	CCATC	3 cut(s)	70, 1591, 2246
BceAI	ACGGC	1 cut(s)	268
BciT130I	CCWGG	4 cut(s)	1012, 1355, 2086, 2130
BclI	TGATCA	2 cut(s)	496, 1411
BcoDI	GTCTC	4 cut(s)	246, 553, 900, 1621
BfaI	CTAG	2 cut(s)	1463, 2222
BfmI	CTRYAG	3 cut(s)	606, 1951, 2237
BfuAI	ACCTGC	1 cut(s)	473
BisI	GCNGC	9 cut(s)	566, 841, 1085, 1346, 1947, 1989, 2028, 2039, 2237
BlsI	GCNGC	9 cut(s)	567, 842, 1086, 1347, 1948, 1990, 2029, 2040, 2238
Bme1390I	CCNGG	4 cut(s)	1012, 1355, 2086, 2130
BmgT120I	GGNCC	1 cut(s)	996
BmiI	GGNNCC	2 cut(s)	1605, 2162
BmrFI	CCNGG	4 cut(s)	1012, 1355, 2086, 2130
Bpu10I	CCTNAGC	1 cut(s)	476
Bpu14I	TTCGAA	1 cut(s)	1037
BsaAI	YACGTR	1 cut(s)	362
BsaBI	GATNNNNATC	1 cut(s)	13
BsaHI	GRCGYC	1 cut(s)	2115
BsaI	GGTCTC	1 cut(s)	246
BsaJI	CCNNGG	5 cut(s)	265, 1794, 1960, 2128, 2129
BsaWI	WCCGGW	1 cut(s)	1865
BsaXI	ACNNNNNCTCC	2 cut(s)	98, 128
Bsc4I	CCNNNNNNNGG	8 cut(s)	277, 860, 1108, 1360, 1809, 2091, 2201, 2269
Bse1I	ACTGG	3 cut(s)	598, 1731, 1767
Bse3DI	GCAATG	4 cut(s)	384, 1483, 1537, 2223
Bse8I	GATNNNNATC	1 cut(s)	13
BseAI	TCCGGA	1 cut(s)	1865
BseBI	CCWGG	4 cut(s)	1012, 1355, 2086, 2130
BseDI	CCNNGG	5 cut(s)	265, 1794, 1960, 2128, 2129
BseJI	GATNNNNATC	1 cut(s)	13
BseLI	CCNNNNNNNGG	8 cut(s)	277, 860, 1108, 1360, 1809, 2091, 2201, 2269
BseMI	GCAATG	4 cut(s)	384, 1483, 1537, 2223
BseMII	CTCAG	6 cut(s)	375, 443, 467, 1091, 1661, 2187
BseNI	ACTGG	3 cut(s)	598, 1731, 1767
BseRI	GAGGAG	1 cut(s)	2240
BseX3I	CGGCCG	1 cut(s)	253
BseXI	GCAGC	7 cut(s)	552, 1357, 1933, 2000, 2014, 2025, 2223
BseYI	CCCAGC	3 cut(s)	553, 732, 1358
Bsh1236I	CGCG	2 cut(s)	353, 1337
Bsh1285I	CGRYCG	1 cut(s)	256
BshFI	GGCC	4 cut(s)	255, 270, 843, 997
BsiEI	CGRYCG	1 cut(s)	256
BsiSI	CCGG	3 cut(s)	256, 296, 1866
BslFI	GGGAC	3 cut(s)	1107, 2111, 2126
BslI	CCNNNNNNNGG	8 cut(s)	277, 860, 1108, 1360, 1809, 2091, 2201, 2269
BsmAI	GTCTC	4 cut(s)	246, 553, 900, 1621
BsmFI	GGGAC	3 cut(s)	1107, 2111, 2126
BsmI	GAATGC	5 cut(s)	622, 1021, 1045, 1327, 1889
BsnI	GGCC	4 cut(s)	255, 270, 843, 997
Bso31I	GGTCTC	1 cut(s)	246
Bsp119I	TTCGAA	1 cut(s)	1037
Bsp13I	TCCGGA	1 cut(s)	1865
Bsp1407I	TGTACA	1 cut(s)	633
Bsp143I	GATC	9 cut(s)	496, 760, 1098, 1173, 1411, 1562, 1603, 2011, 2160
Bsp19I	CCATGG	2 cut(s)	265, 1794
BspACI	CCGC	5 cut(s)	199, 353, 541, 840, 1084
BspANI	GGCC	4 cut(s)	255, 270, 843, 997
BspCNI	CTCAG	6 cut(s)	376, 442, 468, 1090, 1660, 2188
BspEI	TCCGGA	1 cut(s)	1865
BspFNI	CGCG	2 cut(s)	353, 1337
BspLI	GGNNCC	2 cut(s)	1605, 2162
BspMAI	CTGCAG	2 cut(s)	1955, 2241
BspMI	ACCTGC	1 cut(s)	473
BspPI	GGATC	9 cut(s)	768, 1093, 1168, 1570, 1598, 1611, 2006, 2155, 2168
BspQI	GCTCTTC	1 cut(s)	1592
BspT104I	TTCGAA	1 cut(s)	1037
BspTNI	GGTCTC	1 cut(s)	246
BsrDI	GCAATG	4 cut(s)	384, 1483, 1537, 2223
BsrGI	TGTACA	1 cut(s)	633
BsrI	ACTGG	3 cut(s)	598, 1731, 1767
BssECI	CCNNGG	5 cut(s)	265, 1794, 1960, 2128, 2129
BssMI	GATC	9 cut(s)	496, 760, 1098, 1173, 1411, 1562, 1603, 2011, 2160
BssNAI	GTATAC	1 cut(s)	1551
BssNI	GRCGYC	1 cut(s)	2115
BssSI	CACGAG	3 cut(s)	678, 1618, 2033
BssT1I	CCWWGG	2 cut(s)	265, 1794
Bst1107I	GTATAC	1 cut(s)	1551
Bst2BI	CACGAG	3 cut(s)	678, 1618, 2033
Bst2UI	CCWGG	4 cut(s)	1012, 1355, 2086, 2130
Bst4CI	ACNGT	7 cut(s)	27, 93, 976, 1213, 1229, 1495, 1525
Bst6I	CTCTTC	1 cut(s)	1592
BstACI	GRCGYC	1 cut(s)	2115
BstAPI	GCANNNNNTGC	1 cut(s)	617
BstAUI	TGTACA	1 cut(s)	633
BstBAI	YACGTR	1 cut(s)	362
BstBI	TTCGAA	1 cut(s)	1037
BstDEI	CTNAG	6 cut(s)	384, 429, 476, 1077, 1647, 2196
BstDSI	CCRYGG	3 cut(s)	265, 1794, 1960
BstENI	CCTNNNNNAGG	1 cut(s)	2199
BstFNI	CGCG	2 cut(s)	353, 1337
BstHHI	GCGC	2 cut(s)	1337, 1579
BstKTI	GATC	9 cut(s)	499, 763, 1101, 1176, 1414, 1565, 1606, 2014, 2163
BstMAI	GTCTC	4 cut(s)	246, 553, 900, 1621
BstMBI	GATC	9 cut(s)	496, 760, 1098, 1173, 1411, 1562, 1603, 2011, 2160
BstMCI	CGRYCG	1 cut(s)	256
BstMWI	GCNNNNNNNGC	4 cut(s)	152, 617, 830, 2242
BstNI	CCWGG	4 cut(s)	1012, 1355, 2086, 2130
BstNSI	RCATGY	6 cut(s)	331, 572, 940, 1345, 1446, 1549
BstSCI	CCNGG	4 cut(s)	1010, 1353, 2084, 2128
BstSFI	CTRYAG	3 cut(s)	606, 1951, 2237
BstUI	CGCG	2 cut(s)	353, 1337
BstV1I	GCAGC	7 cut(s)	552, 1357, 1933, 2000, 2014, 2025, 2223
BstX2I	RGATCY	4 cut(s)	1173, 1603, 2011, 2160
BstYI	RGATCY	4 cut(s)	1173, 1603, 2011, 2160
BstZ17I	GTATAC	1 cut(s)	1551
BstZI	CGGCCG	1 cut(s)	253
BsuRI	GGCC	4 cut(s)	255, 270, 843, 997
BtgI	CCRYGG	3 cut(s)	265, 1794, 1960
BtsIMutI	CAGTG	4 cut(s)	410, 835, 981, 1724
BveI	ACCTGC	1 cut(s)	473
CaiI	CAGNNNCTG	2 cut(s)	593, 2245
CfoI	GCGC	2 cut(s)	1337, 1579
Cfr13I	GGNCC	1 cut(s)	996
CseI	GACGC	1 cut(s)	505
Csp6I	GTAC	3 cut(s)	424, 634, 1817
CviQI	GTAC	3 cut(s)	424, 634, 1817
DdeI	CTNAG	6 cut(s)	384, 429, 476, 1077, 1647, 2196
DpnI	GATC	9 cut(s)	498, 762, 1100, 1175, 1413, 1564, 1605, 2013, 2162
DpnII	GATC	9 cut(s)	496, 760, 1098, 1173, 1411, 1562, 1603, 2011, 2160
EaeI	YGGCCR	2 cut(s)	253, 268
EagI	CGGCCG	1 cut(s)	253
Eam1104I	CTCTTC	1 cut(s)	1592
EarI	CTCTTC	1 cut(s)	1592
EclXI	CGGCCG	1 cut(s)	253
Eco130I	CCWWGG	2 cut(s)	265, 1794
Eco31I	GGTCTC	1 cut(s)	246
Eco52I	CGGCCG	1 cut(s)	253
Eco57I	CTGAAG	1 cut(s)	2252
EcoNI	CCTNNNNNAGG	1 cut(s)	2199
EcoRII	CCWGG	4 cut(s)	1010, 1353, 2084, 2128
EcoT14I	CCWWGG	2 cut(s)	265, 1794
EcoT22I	ATGCAT	1 cut(s)	1273
ErhI	CCWWGG	2 cut(s)	265, 1794
FaqI	GGGAC	3 cut(s)	1107, 2111, 2126
FauI	CCCGC	1 cut(s)	206
FbaI	TGATCA	2 cut(s)	496, 1411
FblI	GTMKAC	2 cut(s)	55, 1550
Fnu4HI	GCNGC	9 cut(s)	566, 841, 1085, 1346, 1947, 1989, 2028, 2039, 2237
Fsp4HI	GCNGC	9 cut(s)	566, 841, 1085, 1346, 1947, 1989, 2028, 2039, 2237
FspBI	CTAG	2 cut(s)	1463, 2222
FspI	TGCGCA	1 cut(s)	1578
GlaI	GCGC	2 cut(s)	1336, 1578
GluI	GCNGC	9 cut(s)	566, 841, 1085, 1346, 1947, 1989, 2028, 2039, 2237
GsaI	CCCAGC	3 cut(s)	557, 736, 1362
HaeIII	GGCC	4 cut(s)	255, 270, 843, 997
HapII	CCGG	3 cut(s)	256, 296, 1866
HgaI	GACGC	1 cut(s)	505
HhaI	GCGC	2 cut(s)	1337, 1579
Hin1I	GRCGYC	1 cut(s)	2115
Hin6I	GCGC	2 cut(s)	1335, 1577
HinP1I	GCGC	2 cut(s)	1335, 1577
HincII	GTYRAC	4 cut(s)	30, 47, 56, 1849
HindII	GTYRAC	4 cut(s)	30, 47, 56, 1849
HindIII	AAGCTT	3 cut(s)	131, 525, 753
HinfI	GANTC	9 cut(s)	43, 261, 313, 962, 1049, 1474, 1480, 1799, 1936
HpaII	CCGG	3 cut(s)	256, 296, 1866
HphI	GGTGA	3 cut(s)	496, 1058, 1178
Hpy166II	GTNNAC	6 cut(s)	30, 47, 56, 636, 1551, 1849
Hpy8I	GTNNAC	6 cut(s)	30, 47, 56, 636, 1551, 1849
HpyAV	CCTTC	4 cut(s)	854, 1158, 1766, 2025
HpyCH4III	ACNGT	7 cut(s)	27, 93, 976, 1213, 1229, 1495, 1525
HpyCH4IV	ACGT	4 cut(s)	361, 1920, 1968, 2115
HpyF10VI	GCNNNNNNNGC	4 cut(s)	152, 617, 830, 2242
HpyF3I	CTNAG	6 cut(s)	384, 429, 476, 1077, 1647, 2196
HpySE526I	ACGT	4 cut(s)	361, 1920, 1968, 2115
Hsp92I	GRCGYC	1 cut(s)	2115
HspAI	GCGC	2 cut(s)	1335, 1577
Kpn2I	TCCGGA	1 cut(s)	1865
Ksp22I	TGATCA	2 cut(s)	496, 1411
Kzo9I	GATC	9 cut(s)	496, 760, 1098, 1173, 1411, 1562, 1603, 2011, 2160
LguI	GCTCTTC	1 cut(s)	1592
LmnI	GCTCC	2 cut(s)	929, 1439
Lsp1109I	GCAGC	7 cut(s)	552, 1357, 1933, 2000, 2014, 2025, 2223
MaeI	CTAG	2 cut(s)	1463, 2222
MaeII	ACGT	4 cut(s)	361, 1920, 1968, 2115
MaeIII	GTNAC	5 cut(s)	173, 932, 1312, 1519, 1720
MalI	GATC	9 cut(s)	498, 762, 1100, 1175, 1413, 1564, 1605, 2013, 2162
MboI	GATC	9 cut(s)	496, 760, 1098, 1173, 1411, 1562, 1603, 2011, 2160
MboII	GAAGA	5 cut(s)	1298, 1579, 1781, 2052, 2192
MfeI	CAATTG	3 cut(s)	956, 1566, 2068
MflI	RGATCY	4 cut(s)	1173, 1603, 2011, 2160
MlsI	TGGCCA	1 cut(s)	270
MluNI	TGGCCA	1 cut(s)	270
MlyI	GAGTC	2 cut(s)	52, 1930
MmeI	TCCRAC	1 cut(s)	710
Mox20I	TGGCCA	1 cut(s)	270
Mph1103I	ATGCAT	1 cut(s)	1273
MroI	TCCGGA	1 cut(s)	1865
MscI	TGGCCA	1 cut(s)	270
MseI	TTAA	6 cut(s)	11, 582, 1385, 1538, 1631, 2151
MslI	CAYNNNNRTG	2 cut(s)	573, 1901
Msp20I	TGGCCA	1 cut(s)	270
MspA1I	CMGCKG	1 cut(s)	565
MspI	CCGG	3 cut(s)	256, 296, 1866
MspR9I	CCNGG	4 cut(s)	1012, 1355, 2086, 2130
MunI	CAATTG	3 cut(s)	956, 1566, 2068
Mva1269I	GAATGC	5 cut(s)	622, 1021, 1045, 1327, 1889
MvaI	CCWGG	4 cut(s)	1012, 1355, 2086, 2130
MvnI	CGCG	2 cut(s)	353, 1337
MwoI	GCNNNNNNNGC	4 cut(s)	152, 617, 830, 2242
NcoI	CCATGG	2 cut(s)	265, 1794
NdeII	GATC	9 cut(s)	496, 760, 1098, 1173, 1411, 1562, 1603, 2011, 2160
NlaIV	GGNNCC	2 cut(s)	1605, 2162
NmuCI	GTSAC	2 cut(s)	173, 932
NsbI	TGCGCA	1 cut(s)	1578
NsiI	ATGCAT	1 cut(s)	1273
NspI	RCATGY	6 cut(s)	331, 572, 940, 1345, 1446, 1549
NspV	TTCGAA	1 cut(s)	1037
PaeI	GCATGC	2 cut(s)	572, 1345
PasI	CCCWGGG	1 cut(s)	2129
PciI	ACATGT	3 cut(s)	327, 936, 1545
PciSI	GCTCTTC	1 cut(s)	1592
PctI	GAATGC	5 cut(s)	622, 1021, 1045, 1327, 1889
PfeI	GAWTC	7 cut(s)	261, 313, 962, 1049, 1474, 1480, 1799
PflMI	CCANNNNNTGG	1 cut(s)	277
PfoI	TCCNGGA	1 cut(s)	1010
PkrI	GCNGC	9 cut(s)	567, 842, 1086, 1347, 1948, 1990, 2029, 2040, 2238
PleI	GAGTC	2 cut(s)	51, 1930
PpsI	GAGTC	2 cut(s)	51, 1930
Ppu21I	YACGTR	1 cut(s)	362
PscI	ACATGT	3 cut(s)	327, 936, 1545
PshBI	ATTAAT	1 cut(s)	1538
Psp1406I	AACGTT	1 cut(s)	1968
Psp6I	CCWGG	4 cut(s)	1010, 1353, 2084, 2128
PspFI	CCCAGC	3 cut(s)	553, 732, 1358
PspGI	CCWGG	4 cut(s)	1010, 1353, 2084, 2128
PspN4I	GGNNCC	2 cut(s)	1605, 2162
PspPI	GGNCC	1 cut(s)	996
PsrI	GAACNNNNNNTAC	2 cut(s)	626, 658
PstI	CTGCAG	2 cut(s)	1955, 2241
PstNI	CAGNNNCTG	2 cut(s)	593, 2245
PsuI	RGATCY	4 cut(s)	1173, 1603, 2011, 2160
PvuII	CAGCTG	1 cut(s)	565
RsaI	GTAC	3 cut(s)	425, 635, 1818
RsaNI	GTAC	3 cut(s)	424, 634, 1817
RseI	CAYNNNNRTG	2 cut(s)	573, 1901
SalI	GTCGAC	1 cut(s)	54
SapI	GCTCTTC	1 cut(s)	1592
SaqAI	TTAA	6 cut(s)	11, 582, 1385, 1538, 1631, 2151
SatI	GCNGC	9 cut(s)	566, 841, 1085, 1346, 1947, 1989, 2028, 2039, 2237
Sau3AI	GATC	9 cut(s)	496, 760, 1098, 1173, 1411, 1562, 1603, 2011, 2160
Sau96I	GGNCC	1 cut(s)	996
SchI	GAGTC	2 cut(s)	52, 1930
ScrFI	CCNGG	4 cut(s)	1012, 1355, 2086, 2130
SfcI	CTRYAG	3 cut(s)	606, 1951, 2237
SfuI	TTCGAA	1 cut(s)	1037
SmiMI	CAYNNNNRTG	2 cut(s)	573, 1901
SphI	GCATGC	2 cut(s)	572, 1345
SsiI	CCGC	5 cut(s)	199, 353, 541, 840, 1084
SspI	AATATT	1 cut(s)	192
SspMI	CTAG	2 cut(s)	1463, 2222
StyD4I	CCNGG	4 cut(s)	1010, 1353, 2084, 2128
StyI	CCWWGG	2 cut(s)	265, 1794
TaaI	ACNGT	7 cut(s)	27, 93, 976, 1213, 1229, 1495, 1525
TaiI	ACGT	4 cut(s)	364, 1923, 1971, 2118
TaqI	TCGA	5 cut(s)	55, 246, 372, 713, 1037
TaqII	GACCGA	1 cut(s)	635
TatI	WGTACW	1 cut(s)	633
TauI	GCSGC	2 cut(s)	843, 1087
TfiI	GAWTC	7 cut(s)	261, 313, 962, 1049, 1474, 1480, 1799
Tru1I	TTAA	6 cut(s)	11, 582, 1385, 1538, 1631, 2151
Tru9I	TTAA	6 cut(s)	11, 582, 1385, 1538, 1631, 2151
TscAI	CASTG	4 cut(s)	417, 835, 981, 1731
TseFI	GTSAC	2 cut(s)	173, 932
TseI	GCWGC	7 cut(s)	565, 1345, 1946, 1988, 2027, 2038, 2236
Tsp45I	GTSAC	2 cut(s)	173, 932
TspDTI	ATGAA	8 cut(s)	17, 561, 759, 1071, 1472, 1731, 1882, 1893
TspGWI	ACGGA	1 cut(s)	1709
TspRI	CASTG	4 cut(s)	417, 835, 981, 1731
Van91I	CCANNNNNTGG	1 cut(s)	277
VspI	ATTAAT	1 cut(s)	1538
XagI	CCTNNNNNAGG	1 cut(s)	2199
XapI	RAATTY	3 cut(s)	211, 890, 1381
XbaI	TCTAGA	1 cut(s)	1462
XceI	RCATGY	6 cut(s)	331, 572, 940, 1345, 1446, 1549
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2060, 2136
XmiI	GTMKAC	2 cut(s)	55, 1550
XspI	CTAG	2 cut(s)	1463, 2222
ZraI	GACGTC	1 cut(s)	2116
Zsp2I	ATGCAT	1 cut(s)	1273

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.