Rorug03G0097700

Pentatricopeptide repeat-containing protein At3g53360

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0014545
Physical Location & Seq
Reverse (-)
7666827 .. 7668031
1205 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0097700.1

Sequence Viewer

Length: 672 bp
ATGGGAACTTTTACTTTAGACACCATTCTAGTTCCCTTGAGCCTCTTCGTTCTAATAGCTTACCATGCATATTTATGGTGTATCTTCAAGACCAAACCCTCTCACACGACCATCGGAATTGAGTCAGTCAGAAGAAGATTATGGTTTCTAGAGATTATGCAGGGTGATGGTAGAAAGTCTATGTTGGCAGTCCAAAACTTGAGAAACACTCAAATGGTGGCAATAATCACAGCTTCAATAGCAATTGCCTTCAGCTTGGCTTTGGCAGCTCTGACGAACAATGCTTACAATGCAAGCCACCTCTTTGTTAAAACAGCATTTTTCGGCTTGCAGTCGGGGAGGATCTTTACTCTAAAGTATGGCTTGGCCTATTTCGTCTTGCTGTTCAGCTCATTATGCAGCTCCATGGCCACTGGATTTATGATCGATGCCATTTTTCTACTAAACACTTCTGCCGAGTTCTCATCTTCAGGAATAACACTACTCCTATTTGAACGAGGTTACATGTTGGCTCAAATTGGCGAAAGGTTGCTTTTTATCTCCTTTCCTTTGTTGTTGTGGTTGTTTGGTCCAGTGCCAGTGGCCTTGTCATCCCTGGCCTTGGTTTGGTGGCTTAAAGAGCTTGATTTTGTTGGCAAATTCACCCAAAGTAACAGGCAAAGTCTCAGCTGA

Protein Analysis

223

Amino Acids

24.92

Weight (kDa)

9.55

Isoelectric Point (pI)

37.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 9 - 211 2.8e-44 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014545)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53360 AT3G53360
fragaria_vesca FvH4_6g15460
malus_domestica MD04G1103100.v1.1 MD12G1123300.v1.1
prunus_persica Prupe.6G239900_v2.0.a1
pyrus_communis pycom04g09820 pycom12g12200
rosa_chinensis RchiOBHm_Chr3g0468941
rosa_laevigata RLG00000024398
rosa_multiflora Rmu_co8434945.1_g000001
rosa_roxburghii Rroxscaffold_6G00412440
rosa_rugosa Rorug03G0097700
rosa_samantha Rh3AG147500 Rh3BG170100 Rh3DG205100
rosa_wichuraiana Rw3G013870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 350
AcoI YGGCCR 1 cut(s) 408
AcsI RAATTY 1 cut(s) 638
AcuI CTGAAG 2 cut(s) 235, 453
AfiI CCNNNNNNNGG 2 cut(s) 601, 606
AflIII ACRYGT 1 cut(s) 504
AgsI TTSAA 3 cut(s) 88, 237, 494
AjnI CCWGG 1 cut(s) 594
AluBI AGCT 8 cut(s) 59, 233, 255, 269, 390, 402, 622, 669
AluI AGCT 8 cut(s) 59, 233, 255, 269, 390, 402, 622, 669
Alw26I GTCTC 1 cut(s) 668
AlwI GGATC 1 cut(s) 350
AoxI GGCC 4 cut(s) 366, 408, 582, 597
ApeKI GCWGC 2 cut(s) 266, 399
ApoI RAATTY 1 cut(s) 638
AspS9I GGNCC 1 cut(s) 569
AsuHPI GGTGA 2 cut(s) 176, 634
AvaII GGWCC 1 cut(s) 569
BalI TGGCCA 1 cut(s) 410
BbvI GCAGC 2 cut(s) 278, 411
BccI CCATC 2 cut(s) 119, 161
BciT130I CCWGG 1 cut(s) 596
BcoDI GTCTC 1 cut(s) 668
BfaI CTAG 2 cut(s) 29, 149
BisI GCNGC 2 cut(s) 267, 400
BlsI GCNGC 2 cut(s) 268, 401
Bme1390I CCNGG 1 cut(s) 596
Bme18I GGWCC 1 cut(s) 569
BmgT120I GGNCC 1 cut(s) 569
BmrFI CCNGG 1 cut(s) 596
BmsI GCATC 1 cut(s) 418
BplI GAGNNNNNCTC 2 cut(s) 193, 225
BpuEI CTTGAG 2 cut(s) 58, 220
Bsa29I ATCGAT 1 cut(s) 426
BsaJI CCNNGG 3 cut(s) 405, 594, 600
Bsc4I CCNNNNNNNGG 2 cut(s) 601, 606
Bse1I ACTGG 3 cut(s) 418, 572, 578
BseBI CCWGG 1 cut(s) 596
BseCI ATCGAT 1 cut(s) 426
BseDI CCNNGG 3 cut(s) 405, 594, 600
BseGI GGATG 1 cut(s) 590
BseLI CCNNNNNNNGG 2 cut(s) 601, 606
BseNI ACTGG 3 cut(s) 418, 572, 578
BseXI GCAGC 2 cut(s) 278, 411
BshFI GGCC 4 cut(s) 368, 410, 584, 599
BshVI ATCGAT 1 cut(s) 426
BslI CCNNNNNNNGG 2 cut(s) 601, 606
BsmAI GTCTC 1 cut(s) 668
BsnI GGCC 4 cut(s) 368, 410, 584, 599
Bsp143I GATC 2 cut(s) 342, 423
Bsp19I CCATGG 1 cut(s) 405
BspANI GGCC 4 cut(s) 368, 410, 584, 599
BspDI ATCGAT 1 cut(s) 426
BspPI GGATC 1 cut(s) 350
BsrI ACTGG 3 cut(s) 418, 572, 578
BssECI CCNNGG 3 cut(s) 405, 594, 600
BssMI GATC 2 cut(s) 342, 423
BssT1I CCWWGG 2 cut(s) 405, 600
Bst2UI CCWGG 1 cut(s) 596
Bst6I CTCTTC 1 cut(s) 50
BstC8I GCNNGC 2 cut(s) 295, 329
BstDEI CTNAG 1 cut(s) 665
BstDSI CCRYGG 1 cut(s) 405
BstF5I GGATG 1 cut(s) 590
BstKTI GATC 2 cut(s) 345, 426
BstMAI GTCTC 1 cut(s) 668
BstMBI GATC 2 cut(s) 342, 423
BstMWI GCNNNNNNNGC 6 cut(s) 65, 239, 266, 290, 396, 619
BstNI CCWGG 1 cut(s) 596
BstNSI RCATGY 1 cut(s) 508
BstSCI CCNGG 1 cut(s) 594
BstV1I GCAGC 2 cut(s) 278, 411
BstX2I RGATCY 1 cut(s) 342
BstYI RGATCY 1 cut(s) 342
Bsu15I ATCGAT 1 cut(s) 426
BsuRI GGCC 4 cut(s) 368, 410, 584, 599
BsuTUI ATCGAT 1 cut(s) 426
BtgI CCRYGG 1 cut(s) 405
BtsCI GGATG 1 cut(s) 590
BtsIMutI CAGTG 3 cut(s) 411, 579, 585
Cac8I GCNNGC 2 cut(s) 295, 329
Cfr13I GGNCC 1 cut(s) 569
ClaI ATCGAT 1 cut(s) 426
CviAII CATG 3 cut(s) 65, 406, 505
DdeI CTNAG 1 cut(s) 665
DpnI GATC 2 cut(s) 344, 425
DpnII GATC 2 cut(s) 342, 423
EaeI YGGCCR 1 cut(s) 408
Eam1104I CTCTTC 1 cut(s) 50
EarI CTCTTC 1 cut(s) 50
Eco130I CCWWGG 2 cut(s) 405, 600
Eco47I GGWCC 1 cut(s) 569
Eco57I CTGAAG 2 cut(s) 235, 453
EcoRII CCWGG 1 cut(s) 594
EcoT14I CCWWGG 2 cut(s) 405, 600
EcoT22I ATGCAT 1 cut(s) 70
ErhI CCWWGG 2 cut(s) 405, 600
FaeI CATG 3 cut(s) 68, 409, 508
FalI AAGNNNNNCTT 2 cut(s) 347, 379
FatI CATG 3 cut(s) 64, 405, 504
Fnu4HI GCNGC 2 cut(s) 267, 400
FokI GGATG 1 cut(s) 577
Fsp4HI GCNGC 2 cut(s) 267, 400
FspBI CTAG 2 cut(s) 29, 149
GluI GCNGC 2 cut(s) 267, 400
HaeIII GGCC 4 cut(s) 368, 410, 584, 599
Hin1II CATG 3 cut(s) 68, 409, 508
HinfI GANTC 1 cut(s) 122
HphI GGTGA 2 cut(s) 176, 634
Hpy188I TCNGA 3 cut(s) 116, 131, 273
Hpy188III TCNNGA 3 cut(s) 88, 149, 471
HpyAV CCTTC 1 cut(s) 259
HpyCH4V TGCA 5 cut(s) 68, 160, 293, 331, 399
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 239, 266, 290, 396, 619
HpyF3I CTNAG 1 cut(s) 665
Hsp92II CATG 3 cut(s) 68, 409, 508
Kzo9I GATC 2 cut(s) 342, 423
LmnI GCTCC 1 cut(s) 407
LpnPI CCDG 8 cut(s) 146, 399, 456, 581, 585, 591, 608, 640
Lsp1109I GCAGC 2 cut(s) 278, 411
LweI GCATC 1 cut(s) 418
MaeI CTAG 2 cut(s) 29, 149
MaeIII GTNAC 2 cut(s) 500, 650
MalI GATC 2 cut(s) 344, 425
MboI GATC 2 cut(s) 342, 423
MboII GAAGA 5 cut(s) 37, 76, 144, 147, 459
MfeI CAATTG 1 cut(s) 243
MflI RGATCY 1 cut(s) 342
MlsI TGGCCA 1 cut(s) 410
MluCI AATT 4 cut(s) 117, 243, 516, 638
MluNI TGGCCA 1 cut(s) 410
MlyI GAGTC 1 cut(s) 131
MnlI CCTC 5 cut(s) 53, 109, 311, 333, 491
Mox20I TGGCCA 1 cut(s) 410
Mph1103I ATGCAT 1 cut(s) 70
MscI TGGCCA 1 cut(s) 410
MseI TTAA 2 cut(s) 309, 615
MslI CAYNNNNRTG 2 cut(s) 73, 212
Msp20I TGGCCA 1 cut(s) 410
MspA1I CMGCKG 1 cut(s) 669
MspR9I CCNGG 1 cut(s) 596
MunI CAATTG 1 cut(s) 243
MvaI CCWGG 1 cut(s) 596
MwoI GCNNNNNNNGC 6 cut(s) 65, 239, 266, 290, 396, 619
NcoI CCATGG 1 cut(s) 405
NdeII GATC 2 cut(s) 342, 423
NlaIII CATG 3 cut(s) 68, 409, 508
NmeAIII GCCGAG 1 cut(s) 481
NsiI ATGCAT 1 cut(s) 70
NspI RCATGY 1 cut(s) 508
PciI ACATGT 1 cut(s) 504
PkrI GCNGC 2 cut(s) 268, 401
PleI GAGTC 1 cut(s) 130
PpsI GAGTC 1 cut(s) 130
PscI ACATGT 1 cut(s) 504
Psp6I CCWGG 1 cut(s) 594
PspGI CCWGG 1 cut(s) 594
PspPI GGNCC 1 cut(s) 569
PsrI GAACNNNNNNTAC 2 cut(s) 269, 301
PsuI RGATCY 1 cut(s) 342
PvuII CAGCTG 1 cut(s) 669
RseI CAYNNNNRTG 2 cut(s) 73, 212
SaqAI TTAA 2 cut(s) 309, 615
SatI GCNGC 2 cut(s) 267, 400
Sau3AI GATC 2 cut(s) 342, 423
Sau96I GGNCC 1 cut(s) 569
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 1 cut(s) 596
SfaNI GCATC 1 cut(s) 418
SinI GGWCC 1 cut(s) 569
SmiMI CAYNNNNRTG 2 cut(s) 73, 212
SmlI CTYRAG 2 cut(s) 37, 199
SmoI CTYRAG 2 cut(s) 37, 199
Sse9I AATT 4 cut(s) 117, 243, 516, 638
SspMI CTAG 2 cut(s) 29, 149
StyD4I CCNGG 1 cut(s) 594
StyI CCWWGG 2 cut(s) 405, 600
TaqI TCGA 1 cut(s) 426
TasI AATT 4 cut(s) 117, 243, 516, 638
Tru1I TTAA 2 cut(s) 309, 615
Tru9I TTAA 2 cut(s) 309, 615
TscAI CASTG 3 cut(s) 418, 579, 585
TseI GCWGC 2 cut(s) 266, 399
TspRI CASTG 3 cut(s) 418, 579, 585
VpaK11BI GGWCC 1 cut(s) 569
XapI RAATTY 1 cut(s) 638
XbaI TCTAGA 1 cut(s) 148
XceI RCATGY 1 cut(s) 508
XspI CTAG 2 cut(s) 29, 149
Zsp2I ATGCAT 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.