Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0011576

Physical Location & Seq

Genomic Coordinates Reverse (-)

20440739 .. 20447873

Gene Structure 7135 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1129300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2364 bp

ATGTTATTGTCCTTGTTGAGTAAGCGCTCGTTGGAGGCTGATTCCAGGGACTCCTATGGATTTGCTTTGAGACCTCAACATGTACAAAAATATAGAGAGTACTCGAATATCTACAAGGAAGAGGAAGAGGAAAGATCAAATACATGGAAGTACTTCCTTGAAAATATAGAGAAGTCTTCTCAATCGTGCTCTCCTGAGGCAAGTAAAGACGAATTGCCGGCGAAAGCTACTGAGAAGAAAGCAGGGACAGTGTCTGAGAGTGGTGAGGAAGGGAATGATACAATTAGTAAGAAGTCTGTTTCTAATCGTTCATCAGAAAGACATCCAGAAAAGGAGCTTAAACTTTCAGAAGAAACAGAGAAATCTAAGGCCCAAACGTGGATTCTCATTAGATCGTCTCTTAGTTCTATTGAAAATATGATGAGTGTTCGTGTTAGGATGGGAAGAAATATGAAAGATGAGCAGATAATTGGGAATAAAGACCATCTTCCATCCATAGAAGAAGCAGCTTCTTCAGGAGTGGCTTCTGAGGATGACTGTGAGGATAAAATTTGTCTTGACGATGCTTTTAATGAGAGTAAGAACGCCTTAAGTGCAGAAGATTCTGTAAGTGATGGACACTCCGACAGTGTGAAAGTCGTTGGGACAGAAGAGGCTGTTGATGCTGGGACAGAGGAAACTAAGGTTAATGGGAACCCTCCGGAGCCTTTCTTTCCTTGGAAACTAGAACTCGAGTCTCTTGTTCATGGTGGAGTGCCAAGGGATCTCAGGGGAGAGGTATGGCAAGCTTTTGTAGGTGTGAAGGCTCGCCGGGTGGAGAAATATTACTATAACTTGCTGGCTCAAGAAGCAAATACTAGTGAAAGCATGGATAATGACAACTCATCTGGTATTCCCAGAAAATGGAGAAGGCAGATTGAGAAGGACATACCACGTACTTTTCCAGGACATCCTGCATTGAATGAGAATGGTCGTAATTCACTGAGGCGTTTGCTTTTAGCTTATGCTCGTCATAACCCCTCTGTTGGGTATTGCCAGGCAATGAATTTTTTTGCAGGGTTATTGTTACTTCTGATGCCTGAGGAAAATGCCTTTTGGGCTTTGGTTGGTATAATTGACGACTACTTTGATGGCTACTATACTGAGGAAATGATAGAAGCTCAGGTGGACCAACTAGTATTTGAGGACTTGGTGCGAGAAAAATTTCCAAAATTGGTGAATCATCTGGATTTCTTAGGAGTCCAAGTGGGATGGATCATTGGACCTTGGTTCCTTTCCATATTTGTAAATATGATTCCATGGGAAAGTGTTATTCGAGTTTGGGATGTGCTTCTGTTTGAAGGAAATCGTGTTATGCTATTTCGAACAGCACTAGCACTGATGGAGTTATATGGGCCGGCGATAGTTACCACTAAGGATGCAGGAGATGCTATTACTTTGTTGCAGTCCCTTGTTGGCTCAACATTCGATAGCAGTCAGCTCGTTTTGACTGCTTGCATGGGTTTTTTGGCTATAACTGAAAGTAGATTGCAAGAGTTGAGAGAGAAGCATCGACCATCTGTGTTAGCAATTGTAGAGGAAAGATCTAAAAAGGGTCAGGCTTGGAAGGATTCCAAGGGGCTTGCATCTAAGCTGTATAGTTTTAAGCATGATCCCAGTATAATAGAAGAGAAAAGTCCTACTGAAGGAAGTACAGATGGAGATGTACCTCGCTCGAAATCTGGCTCTTCTAATCTGGATGAATTCCTTAGTGTTAGTGGCCTATCTGTATCAGAAGTAGATTCTTTACCGGATCTTCATGATCAGGTCGATTGGTTGAAGGTTGAGGTATGCAGGGTGCTGGAGGAGAAAAGATCTGCTGTACTCAGGGCTGAGGAGTTGGAGACAGCACTTATGGAGATGGTCAAGCAAGATAATCGACGAGAGTTAACCACAAGGGTTGAGCAGTTGGAGCAAGAGGTGGCTGAGCTACGGCAAGCCCTTGCGGATAAGACAGAGCAAGAGACTGCAATGCTTAAGGTCTTGATGTGGTTGGAACAAGAGCAAAAGGTAACTGAAGATGCCCGAGTAAGTGCCGAGCAAGAAGCAGCTGCTCAGAAAAGTGCAGTTAATGTGCTTCAGGAAAAATATGAGAAAGCCATGTCTTCACTTACTGAGATGGAAAAGAAAGTAAAAGTGGCGGAATCAATGTTGGAGGCTACATTGCAATATGAATCCGGTCAAGCCAAAGCGCTTTCTCCAAGGGCAGCTAACACACAGGGAAACTCCACAAAAAGGATGGGCATTCTACCATTTGGACTTGGTTGGGGTGACAGAAATAAGGGATCCAGTGATCTGAAATCACCGAATCAACAGAGAGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005096 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0006810 GO:0006886 GO:0008047 GO:0008104 GO:0008150 GO:0012505 GO:0015031 GO:0015833 GO:0017016 GO:0017137 GO:0019899 GO:0030234 GO:0030695 GO:0031267 GO:0031338 GO:0032879 GO:0033036 GO:0033043 GO:0034613 GO:0042886 GO:0043085 GO:0043087 GO:0043547 GO:0044093 GO:0044464 GO:0045184 GO:0046907 GO:0050789 GO:0050790 GO:0050794 GO:0051020 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0060589 GO:0060627 GO:0065007 GO:0065009 GO:0070727 GO:0071702 GO:0071705 GO:0090630 GO:0098772

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RabGAP-TBC

Protein Analysis

788

Amino Acids

88.55

Weight (kDa)

5.0

Isoelectric Point (pI)

55.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RabGAP-TBC	PF00566	255 - 466	1.5e-55	Rab-GTPase-TBC domain
RabGap-TBC_2	PF23436	327 - 489	9.5e-26	RabGAP TBC domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011576)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g15020
malus_domestica	MD04G1112400.v1.1 MD12G1129300.v1.1
prunus_persica	Prupe.6G244600_v2.0.a1 Prupe.6G244600_v2.0.a1 Prupe.6G244600_v2.0.a1 Prupe.6G244600_v2.0.a1 Prupe.6G244600_v2.0.a1
pyrus_communis	pycom04g10320 pycom12g12560
rosa_chinensis	RchiOBHm_Chr3g0468201
rosa_laevigata	RLG00000024442
rosa_multiflora	Rmu_sc0004524.1_g000007
rosa_roxburghii	Rroxscaffold_6G00412920
rosa_rugosa	Rorug03G0094100
rosa_samantha	Rh3AG141800 Rh3BG163200 Rh3CG163300 Rh3DG163900
rosa_wichuraiana	Rw3G013420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	903
AccIII	TCCGGA	1 cut(s)	700
AciI	CCGC	2 cut(s)	1985, 2180
AclWI	GGATC	6 cut(s)	771, 1262, 1646, 1800, 2319, 2332
AcsI	RAATTY	4 cut(s)	549, 1045, 1202, 1742
AcuI	CTGAAG	4 cut(s)	498, 1704, 2076, 2102
AfaI	GTAC	7 cut(s)	84, 101, 152, 937, 1693, 1707, 1863
AfeI	AGCGCT	2 cut(s)	26, 2232
AfiI	CCNNNNNNNGG	6 cut(s)	378, 903, 1025, 1026, 1685, 2274
AflII	CTTAAG	2 cut(s)	589, 2015
AflIII	ACRYGT	1 cut(s)	79
AgsI	TTSAA	5 cut(s)	161, 413, 961, 1340, 1819
AhlI	ACTAGT	2 cut(s)	857, 1174
AjnI	CCWGG	3 cut(s)	44, 943, 1035
AjuI	GAANNNNNNNTTGG	2 cut(s)	366, 398
Alw21I	GWGCWC	1 cut(s)	191
Alw26I	GTCTC	6 cut(s)	64, 402, 741, 1877, 1997, 2352
AlwI	GGATC	6 cut(s)	771, 1262, 1646, 1800, 2319, 2332
AlwNI	CAGNNNCTG	1 cut(s)	254
Ama87I	CYCGRG	2 cut(s)	731, 2064
Aor13HI	TCCGGA	1 cut(s)	700
Aor51HI	AGCGCT	2 cut(s)	26, 2232
AoxI	GGCC	3 cut(s)	369, 1394, 1759
ApeKI	GCWGC	4 cut(s)	506, 2087, 2090, 2246
ApoI	RAATTY	4 cut(s)	549, 1045, 1202, 1742
ArsI	GACNNNNNNTTYG	2 cut(s)	1109, 1141
Asp700I	GAANNNNTTC	2 cut(s)	152, 1203
AspLEI	GCGC	2 cut(s)	27, 2233
AspS9I	GGNCC	4 cut(s)	370, 1168, 1262, 1394
AsuC2I	CCSGG	1 cut(s)	812
AsuHPI	GGTGA	4 cut(s)	275, 1228, 2321, 2334
AsuII	TTCGAA	1 cut(s)	1363
AvaI	CYCGRG	2 cut(s)	731, 2064
AvaII	GGWCC	2 cut(s)	1168, 1262
AxyI	CCTNAGG	2 cut(s)	195, 1080
BamHI	GGATCC	1 cut(s)	2324
BbsI	GAAGAC	2 cut(s)	168, 2136
Bbv12I	GWGCWC	1 cut(s)	191
BbvCI	CCTCAGC	1 cut(s)	1872
BbvI	GCAGC	4 cut(s)	518, 2077, 2099, 2258
BceAI	ACGGC	1 cut(s)	1988
BcgI	CGANNNNNNTGC	4 cut(s)	1462, 1496, 1898, 1932
BciT130I	CCWGG	3 cut(s)	46, 945, 1037
BclI	TGATCA	1 cut(s)	1801
BcnI	CCSGG	1 cut(s)	812
BcoDI	GTCTC	6 cut(s)	64, 402, 741, 1877, 1997, 2352
BcuI	ACTAGT	2 cut(s)	857, 1174
BfaI	CTAG	4 cut(s)	725, 858, 1175, 1373
BfoI	RGCGCY	2 cut(s)	28, 2234
BfrI	CTTAAG	2 cut(s)	589, 2015
BglI	GCCNNNNNGGC	1 cut(s)	1097
BglII	AGATCT	2 cut(s)	1583, 1853
BisI	GCNGC	4 cut(s)	507, 2088, 2091, 2247
BlpI	GCTNAGC	1 cut(s)	1965
BlsI	GCNGC	4 cut(s)	508, 2089, 2092, 2248
BmcAI	AGTACT	2 cut(s)	101, 152
Bme1390I	CCNGG	4 cut(s)	46, 812, 945, 1037
Bme18I	GGWCC	2 cut(s)	1168, 1262
BmeT110I	CYCGRG	2 cut(s)	731, 2064
BmgT120I	GGNCC	4 cut(s)	370, 1168, 1262, 1394
BmiI	GGNNCC	4 cut(s)	695, 705, 1271, 2326
BmrFI	CCNGG	4 cut(s)	46, 812, 945, 1037
BmrI	ACTGGG	1 cut(s)	1650
BmsI	GCATC	8 cut(s)	553, 652, 1065, 1408, 1417, 1558, 1634, 2050
BmuI	ACTGGG	1 cut(s)	1650
BpiI	GAAGAC	2 cut(s)	168, 2136
BpmI	CTGGAG	1 cut(s)	1862
Bpu10I	CCTNAGC	2 cut(s)	1161, 1872
Bpu1102I	GCTNAGC	1 cut(s)	1965
Bpu14I	TTCGAA	1 cut(s)	1363
BpuEI	CTTGAG	1 cut(s)	828
BpuMI	CCSGG	1 cut(s)	812
BsaAI	YACGTR	1 cut(s)	935
BsaI	GGTCTC	1 cut(s)	64
BsaJI	CCNNGG	7 cut(s)	45, 716, 758, 1265, 1298, 1614, 2240
BsaWI	WCCGGW	3 cut(s)	700, 1789, 2216
BsaXI	ACNNNNNCTCC	2 cut(s)	1230, 1260
Bsc4I	CCNNNNNNNGG	6 cut(s)	378, 903, 1025, 1026, 1685, 2274
Bse118I	RCCGGY	2 cut(s)	217, 1396
Bse1I	ACTGG	2 cut(s)	1656, 2328
Bse21I	CCTNAGG	2 cut(s)	195, 1080
Bse3DI	GCAATG	3 cut(s)	1047, 2016, 2201
BseAI	TCCGGA	1 cut(s)	700
BseBI	CCWGG	3 cut(s)	46, 945, 1037
BseDI	CCNNGG	7 cut(s)	45, 716, 758, 1265, 1298, 1614, 2240
BseLI	CCNNNNNNNGG	6 cut(s)	378, 903, 1025, 1026, 1685, 2274
BseMI	GCAATG	3 cut(s)	1047, 2016, 2201
BseNI	ACTGG	2 cut(s)	1656, 2328
BseRI	GAGGAG	2 cut(s)	1859, 1889
BseXI	GCAGC	4 cut(s)	518, 2077, 2099, 2258
BseYI	CCCAGC	1 cut(s)	665
BsgI	GTGCAG	2 cut(s)	615, 2124
BshFI	GGCC	3 cut(s)	371, 1396, 1761
BsiHKAI	GWGCWC	1 cut(s)	191
BsiHKCI	CYCGRG	2 cut(s)	731, 2064
BsiSI	CCGG	6 cut(s)	218, 701, 811, 1397, 1790, 2217
BslFI	GGGAC	5 cut(s)	62, 259, 658, 682, 1432
BslI	CCNNNNNNNGG	6 cut(s)	378, 903, 1025, 1026, 1685, 2274
BsmAI	GTCTC	6 cut(s)	64, 402, 741, 1877, 1997, 2352
BsmBI	CGTCTC	1 cut(s)	402
BsmFI	GGGAC	5 cut(s)	62, 259, 658, 682, 1432
BsmI	GAATGC	1 cut(s)	2283
BsnI	GGCC	3 cut(s)	371, 1396, 1761
Bso31I	GGTCTC	1 cut(s)	64
BsoBI	CYCGRG	2 cut(s)	731, 2064
Bsp119I	TTCGAA	1 cut(s)	1363
Bsp1286I	GDGCHC	1 cut(s)	191
Bsp13I	TCCGGA	1 cut(s)	700
Bsp1407I	TGTACA	1 cut(s)	82
Bsp1720I	GCTNAGC	1 cut(s)	1965
Bsp19I	CCATGG	1 cut(s)	1298
BspACI	CCGC	2 cut(s)	1985, 2180
BspANI	GGCC	3 cut(s)	371, 1396, 1761
BspEI	TCCGGA	1 cut(s)	700
BspHI	TCATGA	1 cut(s)	1798
BspLI	GGNNCC	4 cut(s)	695, 705, 1271, 2326
BspPI	GGATC	6 cut(s)	771, 1262, 1646, 1800, 2319, 2332
BspQI	GCTCTTC	1 cut(s)	1732
BspT104I	TTCGAA	1 cut(s)	1363
BspTI	CTTAAG	2 cut(s)	589, 2015
BspTNI	GGTCTC	1 cut(s)	64
BsrDI	GCAATG	3 cut(s)	1047, 2016, 2201
BsrFI	RCCGGY	2 cut(s)	217, 1396
BsrGI	TGTACA	1 cut(s)	82
BsrI	ACTGG	2 cut(s)	1656, 2328
BssAI	RCCGGY	2 cut(s)	217, 1396
BssECI	CCNNGG	7 cut(s)	45, 716, 758, 1265, 1298, 1614, 2240
BssT1I	CCWWGG	6 cut(s)	716, 758, 1265, 1298, 1614, 2240
Bst2UI	CCWGG	3 cut(s)	46, 945, 1037
Bst4CI	ACNGT	3 cut(s)	250, 539, 629
Bst6I	CTCTTC	5 cut(s)	114, 120, 645, 1662, 1732
BstAFI	CTTAAG	2 cut(s)	589, 2015
BstAPI	GCANNNNNTGC	1 cut(s)	1427
BstAUI	TGTACA	1 cut(s)	82
BstBAI	YACGTR	1 cut(s)	935
BstBI	TTCGAA	1 cut(s)	1363
BstC8I	GCNNGC	8 cut(s)	219, 786, 808, 840, 1398, 1495, 1623, 1977
BstDSI	CCRYGG	1 cut(s)	1298
BstENI	CCTNNNNNAGG	1 cut(s)	1683
BstH2I	RGCGCY	2 cut(s)	28, 2234
BstHHI	GCGC	2 cut(s)	27, 2233
BstMAI	GTCTC	6 cut(s)	64, 402, 741, 1877, 1997, 2352
BstMWI	GCNNNNNNNGC	6 cut(s)	593, 662, 848, 1097, 1427, 1951
BstNI	CCWGG	3 cut(s)	46, 945, 1037
BstNSI	RCATGY	1 cut(s)	83
BstSCI	CCNGG	4 cut(s)	44, 810, 943, 1035
BstV1I	GCAGC	4 cut(s)	518, 2077, 2099, 2258
BstV2I	GAAGAC	2 cut(s)	168, 2136
BstX2I	RGATCY	5 cut(s)	763, 1583, 1792, 1853, 2324
BstYI	RGATCY	5 cut(s)	763, 1583, 1792, 1853, 2324
Bsu36I	CCTNAGG	2 cut(s)	195, 1080
BsuRI	GGCC	3 cut(s)	371, 1396, 1761
BtgI	CCRYGG	1 cut(s)	1298
BtsIMutI	CAGTG	5 cut(s)	255, 634, 980, 1376, 2335
Cac8I	GCNNGC	8 cut(s)	219, 786, 808, 840, 1398, 1495, 1623, 1977
CaiI	CAGNNNCTG	1 cut(s)	254
CciI	TCATGA	1 cut(s)	1798
CfoI	GCGC	2 cut(s)	27, 2233
Cfr10I	RCCGGY	2 cut(s)	217, 1396
Cfr13I	GGNCC	4 cut(s)	370, 1168, 1262, 1394
Csp6I	GTAC	7 cut(s)	83, 100, 151, 936, 1692, 1706, 1862
CspCI	CAANNNNNGTGG	2 cut(s)	1921, 1956
CviAII	CATG	9 cut(s)	80, 144, 746, 868, 1299, 1498, 1649, 1799, 2140
CviQI	GTAC	7 cut(s)	83, 100, 151, 936, 1692, 1706, 1862
Eam1104I	CTCTTC	5 cut(s)	114, 120, 645, 1662, 1732
EarI	CTCTTC	5 cut(s)	114, 120, 645, 1662, 1732
EciI	GGCGGA	1 cut(s)	2195
Eco130I	CCWWGG	6 cut(s)	716, 758, 1265, 1298, 1614, 2240
Eco31I	GGTCTC	1 cut(s)	64
Eco47I	GGWCC	2 cut(s)	1168, 1262
Eco47III	AGCGCT	2 cut(s)	26, 2232
Eco57I	CTGAAG	4 cut(s)	498, 1704, 2076, 2102
Eco81I	CCTNAGG	2 cut(s)	195, 1080
Eco88I	CYCGRG	2 cut(s)	731, 2064
EcoNI	CCTNNNNNAGG	1 cut(s)	1683
EcoRI	GAATTC	1 cut(s)	1742
EcoRII	CCWGG	3 cut(s)	44, 943, 1035
EcoT14I	CCWWGG	6 cut(s)	716, 758, 1265, 1298, 1614, 2240
ErhI	CCWWGG	6 cut(s)	716, 758, 1265, 1298, 1614, 2240
Esp3I	CGTCTC	1 cut(s)	402
FaeI	CATG	9 cut(s)	83, 147, 749, 871, 1302, 1501, 1652, 1802, 2143
FalI	AAGNNNNNCTT	4 cut(s)	471, 503, 572, 604
FaqI	GGGAC	5 cut(s)	62, 259, 658, 682, 1432
FatI	CATG	9 cut(s)	79, 143, 745, 867, 1298, 1497, 1648, 1798, 2139
FbaI	TGATCA	1 cut(s)	1801
Fnu4HI	GCNGC	4 cut(s)	507, 2088, 2091, 2247
Fsp4HI	GCNGC	4 cut(s)	507, 2088, 2091, 2247
FspBI	CTAG	4 cut(s)	725, 858, 1175, 1373
GlaI	GCGC	2 cut(s)	26, 2232
GluI	GCNGC	4 cut(s)	507, 2088, 2091, 2247
GsaI	CCCAGC	1 cut(s)	669
GsuI	CTGGAG	1 cut(s)	1862
HaeII	RGCGCY	2 cut(s)	28, 2234
HaeIII	GGCC	3 cut(s)	371, 1396, 1761
HapII	CCGG	6 cut(s)	218, 701, 811, 1397, 1790, 2217
HhaI	GCGC	2 cut(s)	27, 2233
Hin1II	CATG	9 cut(s)	83, 147, 749, 871, 1302, 1501, 1652, 1802, 2143
Hin6I	GCGC	2 cut(s)	25, 2231
HinP1I	GCGC	2 cut(s)	25, 2231
HincII	GTYRAC	1 cut(s)	1929
HindII	GTYRAC	1 cut(s)	1929
HindIII	AAGCTT	1 cut(s)	786
HpaI	GTTAAC	1 cut(s)	1929
HpaII	CCGG	6 cut(s)	218, 701, 811, 1397, 1790, 2217
HphI	GGTGA	4 cut(s)	275, 1228, 2321, 2334
Hpy166II	GTNNAC	2 cut(s)	1168, 1929
Hpy188I	TCNGA	9 cut(s)	256, 316, 349, 529, 625, 1074, 1774, 2097, 2337
Hpy8I	GTNNAC	2 cut(s)	1168, 1929
Hpy99I	CGWCG	1 cut(s)	1923
HpyAV	CCTTC	8 cut(s)	263, 796, 903, 916, 1334, 1600, 1679, 1813
HpyCH4III	ACNGT	3 cut(s)	250, 539, 629
HpyCH4IV	ACGT	2 cut(s)	377, 934
HpyF10VI	GCNNNNNNNGC	6 cut(s)	593, 662, 848, 1097, 1427, 1951
HpySE526I	ACGT	2 cut(s)	377, 934
Hsp92II	CATG	9 cut(s)	83, 147, 749, 871, 1302, 1501, 1652, 1802, 2143
HspAI	GCGC	2 cut(s)	25, 2231
Kpn2I	TCCGGA	1 cut(s)	700
KroI	GCCGGC	2 cut(s)	217, 1396
KroNI	GCCGGC	2 cut(s)	219, 1398
Ksp22I	TGATCA	1 cut(s)	1801
KspAI	GTTAAC	1 cut(s)	1929
LguI	GCTCTTC	1 cut(s)	1732
LmnI	GCTCC	3 cut(s)	334, 703, 1951
Lsp1109I	GCAGC	4 cut(s)	518, 2077, 2099, 2258
LweI	GCATC	8 cut(s)	553, 652, 1065, 1408, 1417, 1558, 1634, 2050
MaeI	CTAG	4 cut(s)	725, 858, 1175, 1373
MaeII	ACGT	2 cut(s)	377, 934
MaeIII	GTNAC	4 cut(s)	1065, 1405, 2050, 2309
MfeI	CAATTG	1 cut(s)	1569
MflI	RGATCY	5 cut(s)	763, 1583, 1792, 1853, 2324
MhlI	GDGCHC	1 cut(s)	191
MlyI	GAGTC	3 cut(s)	44, 743, 1248
MmeI	TCCRAC	6 cut(s)	12, 648, 1860, 1929, 2013, 2172
MroI	TCCGGA	1 cut(s)	700
MroNI	GCCGGC	2 cut(s)	217, 1396
MroXI	GAANNNNTTC	2 cut(s)	152, 1203
MseI	TTAA	8 cut(s)	339, 570, 590, 687, 1644, 1928, 2016, 2109
MspA1I	CMGCKG	1 cut(s)	2090
MspCI	CTTAAG	2 cut(s)	589, 2015
MspI	CCGG	6 cut(s)	218, 701, 811, 1397, 1790, 2217
MspR9I	CCNGG	4 cut(s)	46, 812, 945, 1037
MunI	CAATTG	1 cut(s)	1569
Mva1269I	GAATGC	1 cut(s)	2283
MvaI	CCWGG	3 cut(s)	46, 945, 1037
MwoI	GCNNNNNNNGC	6 cut(s)	593, 662, 848, 1097, 1427, 1951
NaeI	GCCGGC	2 cut(s)	219, 1398
NciI	CCSGG	1 cut(s)	812
NcoI	CCATGG	1 cut(s)	1298
NgoMIV	GCCGGC	2 cut(s)	217, 1396
NlaIII	CATG	9 cut(s)	83, 147, 749, 871, 1302, 1501, 1652, 1802, 2143
NlaIV	GGNNCC	4 cut(s)	695, 705, 1271, 2326
NmeAIII	GCCGAG	1 cut(s)	2101
NmuCI	GTSAC	1 cut(s)	2309
NspI	RCATGY	1 cut(s)	83
NspV	TTCGAA	1 cut(s)	1363
PaeR7I	CTCGAG	1 cut(s)	731
PagI	TCATGA	1 cut(s)	1798
PciI	ACATGT	1 cut(s)	79
PciSI	GCTCTTC	1 cut(s)	1732
PctI	GAATGC	1 cut(s)	2283
PdiI	GCCGGC	2 cut(s)	219, 1398
PdmI	GAANNNNTTC	2 cut(s)	152, 1203
PflFI	GACNNNGTC	1 cut(s)	250
PflMI	CCANNNNNTGG	1 cut(s)	903
PfoI	TCCNGGA	1 cut(s)	943
PkrI	GCNGC	4 cut(s)	508, 2089, 2092, 2248
PleI	GAGTC	3 cut(s)	44, 742, 1247
PpsI	GAGTC	3 cut(s)	44, 742, 1247
Ppu21I	YACGTR	1 cut(s)	935
PscI	ACATGT	1 cut(s)	79
Psp6I	CCWGG	3 cut(s)	44, 943, 1035
PspFI	CCCAGC	1 cut(s)	665
PspGI	CCWGG	3 cut(s)	44, 943, 1035
PspN4I	GGNNCC	4 cut(s)	695, 705, 1271, 2326
PspPI	GGNCC	4 cut(s)	370, 1168, 1262, 1394
PspXI	VCTCGAGB	1 cut(s)	731
PstNI	CAGNNNCTG	1 cut(s)	254
PsuI	RGATCY	5 cut(s)	763, 1583, 1792, 1853, 2324
PsyI	GACNNNGTC	1 cut(s)	250
PvuII	CAGCTG	1 cut(s)	2090
RsaI	GTAC	7 cut(s)	84, 101, 152, 937, 1693, 1707, 1863
RsaNI	GTAC	7 cut(s)	83, 100, 151, 936, 1692, 1706, 1862
SapI	GCTCTTC	1 cut(s)	1732
SaqAI	TTAA	8 cut(s)	339, 570, 590, 687, 1644, 1928, 2016, 2109
SatI	GCNGC	4 cut(s)	507, 2088, 2091, 2247
Sau96I	GGNCC	4 cut(s)	370, 1168, 1262, 1394
ScaI	AGTACT	2 cut(s)	101, 152
SchI	GAGTC	3 cut(s)	44, 743, 1248
ScrFI	CCNGG	4 cut(s)	46, 812, 945, 1037
SduI	GDGCHC	1 cut(s)	191
SfaNI	GCATC	8 cut(s)	553, 652, 1065, 1408, 1417, 1558, 1634, 2050
Sfr274I	CTCGAG	1 cut(s)	731
SfuI	TTCGAA	1 cut(s)	1363
SinI	GGWCC	2 cut(s)	1168, 1262
SlaI	CTCGAG	1 cut(s)	731
SmlI	CTYRAG	4 cut(s)	589, 731, 843, 2015
SmoI	CTYRAG	4 cut(s)	589, 731, 843, 2015
SpeI	ACTAGT	2 cut(s)	857, 1174
SsiI	CCGC	2 cut(s)	1985, 2180
SspI	AATATT	1 cut(s)	824
SspMI	CTAG	4 cut(s)	725, 858, 1175, 1373
StyD4I	CCNGG	4 cut(s)	44, 810, 943, 1035
StyI	CCWWGG	6 cut(s)	716, 758, 1265, 1298, 1614, 2240
TaaI	ACNGT	3 cut(s)	250, 539, 629
TaiI	ACGT	2 cut(s)	380, 937
TaqI	TCGA	9 cut(s)	104, 732, 1315, 1363, 1467, 1552, 1715, 1809, 1918
TatI	WGTACW	5 cut(s)	82, 99, 150, 1691, 1861
Tru1I	TTAA	8 cut(s)	339, 570, 590, 687, 1644, 1928, 2016, 2109
Tru9I	TTAA	8 cut(s)	339, 570, 590, 687, 1644, 1928, 2016, 2109
TscAI	CASTG	5 cut(s)	255, 634, 987, 1383, 2335
TseFI	GTSAC	1 cut(s)	2309
TseI	GCWGC	4 cut(s)	506, 2087, 2090, 2246
Tsp45I	GTSAC	1 cut(s)	2309
TspDTI	ATGAA	7 cut(s)	300, 467, 734, 1058, 1755, 1787, 2226
TspRI	CASTG	5 cut(s)	255, 634, 987, 1383, 2335
Tth111I	GACNNNGTC	1 cut(s)	250
Van91I	CCANNNNNTGG	1 cut(s)	903
Vha464I	CTTAAG	2 cut(s)	589, 2015
VpaK11BI	GGWCC	2 cut(s)	1168, 1262
XagI	CCTNNNNNAGG	1 cut(s)	1683
XapI	RAATTY	4 cut(s)	549, 1045, 1202, 1742
XceI	RCATGY	1 cut(s)	83
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2275
XhoI	CTCGAG	1 cut(s)	731
XmnI	GAANNNNTTC	2 cut(s)	152, 1203
XspI	CTAG	4 cut(s)	725, 858, 1175, 1373
ZrmI	AGTACT	2 cut(s)	101, 152

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.