MD12G1138700.v1.1

TPL-binding domain in jasmonate signalling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009150
Physical Location & Seq
Reverse (-)
21603320 .. 21609999
6680 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1138700.v1.1.491

Sequence Viewer

Length: 2538 bp
ATGGGAGAAGAGGCAATTTGTGTGGGGAAATTGAGAGATGGGGAAGTGGAAACAGAAGACTTCCCGAGAACTGAACTGAAGCGGGAGCACGAGTGTCTCGAAGATAATAGCGGGGCAGACGGGTTTCCAAACAAGAAGCAAGTGAAGGAGCATTCCAATGAGGATATAAGATCGGAAGTATCAAACCCCGTTGTGTCTCCGAAAGAGAACAGCTCCACTTTTCAGGACATTACTAGCCAACCTGCGGAATTGGTAAATAGTAGCGGAACAGAGTGTGGTGAAGTTGAGTCCTCATGTTCGGGGAATTCGGGTGCAGAGGAGACCTTGAGCAATGGACAGTGTACCGAGAAAGATAATTGTCAAATTGAGAACGAGACGAATGGTGATGTATTGACCTCTCGTGTTGTGGTAGAAATTCCTAAGCTTGCCAGTTCATCTGGGATTCGCAAGATTACGTTCAAGTTCAGCAAGAAAAAGGAGAATCAGACTGCGAGTAATCAAGACCTTTCACATGGAGGTTCACACGAGGAGCCCGGAATGGACTTTCAAGCGATGCCTAGTACAAGTAGGGAGTTTCCAGCTAGTTCATATTGGAGGCAATGCGCTGAAACCGGCAACTTTCATCCTTGTGCGCTTAATTCAAAGTTGGAAAGTTCTAACTATGTAGTTCCGAGCAGCTATCCTTCAAATGTTAAGAAGCTATTGTCTACTGGTATTCTTGATGGAGCTCGAGTGACGTATGTTTCCTCCCCGCCAAAGATAGAGCTACATGGAATTATAAGTGGCGGTGGTTATTTGTGCGGCTGCTCATCATGCAATTTCACCAGGGTACTCAGTGCATATGAGTTTGAGCAGCATGCTGGTGTCAAGACTCGGCATCCTAACAATCACATATACATGGAGAATGGGAGGGCAGTTTATAGCATCATACAAGAACTGAAGACTGCTCCGCTAAGCAGTCTGGATGAAGTGATAAAGGATATTGCTGGTTCTGCTCTCAATGAAGAGTCCTTCCGGGTTTGGAAAGAAACTCTTAATCGAAGTGATGGAATGGCTGAAGTGGAGAAAAGACATCGTGTGAAGCTTCCTAAATTGCGTCATCCAATTCCCAGACCAAGCTTCCACAATCCGTATGCAGCTATGTACCAGAAGAAAACTGCTGAAGGTGGCAATAGGAAAAGGGATAATGATCTCCACCGGTTACTATTTATGCCAAATGGACTTCCAGATGGAGCTAAATTGGCATACTATATCAAGGGGCAGAGATTACTTAGTGGCTATAAGCAGGGAAATGGGATAGTCTGTAATTGCTGTGACAAAGAGATAAGCCCTTCACAGTTTGAAGCTCATGCTGGAATGGCTGCAAGGCGTCAACCCTACCGTCACATCTATATTTCCAATGGACTGACACTTCACGATATAGCCATGTCATTGGCCAATGGCCAAAACCTGACAATTGGTGGCAGTGATGGCAATGATGATATGTGTGCTGTATGTGGACATGATACAGGGGATATGATTTTCTGTGATGGATGCCCTCGTGCTTATCATTCAGCTTGTTTGGATTTACCACGGGTTCCTCAAGGTGATTGGCATTGTCCAGATTGTAGAGATAAGTTTGAACCTGGTAGAAAAGCTGCTGCTGGGGAATCTTCAAATTTTGGAAGACCTATTGTAATACGGTTGACACGGGTCTTCAATGCACCTGAGTATGAAATTGGTGGCTGCGTTGTTTGCAGGAGCCATGATTTCAGTGCGGCTTTATTTGATGAACGAACTGTAATAATCTGTGATCAGTGTGAGAAGGAGTTCCATGTTGGCTGCTTACGAAACAGTGGATTATGTGACTTAAAAGAACTCCCCAAAGATAAATGGTTTTGTTGTGATGACTGTAACAGGATTCATGCAGCTTTACAGAATTTAGTACTCAGTGAAGCAGAGCATGTTCCAGCTCCATTATCAGATACAATAATCCGGAAGCATGCAGACAGAGGCATATTTATTGATGGAATGGCAGATGTCCAATGGCGAGTTTTCAGTGGAAAGAGGCGCTATCCGGAACACCTACCCTTCCTTTCAAGGGCTGCTGCTATTTTTCGAGAGTGCTTTGACCCTATTGTTGCACAATCTGGTCGTGACTTGATCCCTGTCATGGTATATGGGAGAAATATTTCAGGTCAAGAGTTTGGAGGCATGTACTGTGTAGTTTTAATAGTGGGGTCTGTTGTTGTATCTGCTGGTCTCCTTAGGGTTTTTGGTCGGGAGGTTGCTGAGCTTCCTATTGTTGCTACTAGTAGAGAACATCAAGGAAAAGGTTATTTCCAAGCATTATTTTCATGCATAGAGAGGTTACTTATTTCCCTGAAAGTAGAAAAACTGGTTCTCCCCGCAGCTGAGGAAGCTGAGTCAATCTGGACGAGGAAATTAGGATTTAGAAAGATGAGAGACGAGCAGTTATCGAAATACATGAGGGAAGTGCAGTTGACAATTTTCAGGGGGACGTCAATGCTAGAAAAGGTGGTGAAGTTGACGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas PHD

Protein Analysis

846

Amino Acids

93.76

Weight (kDa)

6.26

Isoelectric Point (pI)

42.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 258 - 312 1.1e-13 Tify domain binding domain
TDBD PF16135 429 - 474 1e-13 Tify domain binding domain
PHD-1st_NSD PF23011 493 - 537 7.1e-07 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger 1
PHD PF00628 496 - 536 1.3e-08 PHD-finger
IDM1_C PF23209 703 - 839 4.7e-51 Increased DNA methylation 1, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 779
AatII GACGTC 1 cut(s) 2504
Acc36I ACCTGC 1 cut(s) 250
AccI GTMKAC 1 cut(s) 707
AccIII TCCGGA 2 cut(s) 1974, 2056
AclWI GGATC 1 cut(s) 2137
AcoI YGGCCR 2 cut(s) 1434, 1441
AcsI RAATTY 4 cut(s) 304, 414, 1657, 1918
AcuI CTGAAG 4 cut(s) 98, 959, 1077, 1182
AcyI GRCGYC 2 cut(s) 1369, 2501
AfaI GTAC 6 cut(s) 343, 562, 831, 1145, 1926, 2198
AfiI CCNNNNNNNGG 3 cut(s) 244, 2080, 2152
AgeI ACCGGT 1 cut(s) 1197
AgsI TTSAA 9 cut(s) 460, 548, 642, 687, 1343, 1622, 1656, 1699, 2079
AhlI ACTAGT 1 cut(s) 2291
AjnI CCWGG 2 cut(s) 824, 1624
Alw21I GWGCWC 2 cut(s) 90, 730
Alw26I GTCTC 6 cut(s) 101, 201, 314, 368, 2246, 2439
AlwI GGATC 1 cut(s) 2137
Ama87I CYCGRG 2 cut(s) 64, 729
Aor13HI TCCGGA 2 cut(s) 1974, 2056
AoxI GGCC 2 cut(s) 1434, 1441
ApoI RAATTY 4 cut(s) 304, 414, 1657, 1918
AsiGI ACCGGT 1 cut(s) 1197
Asp700I GAANNNNTTC 2 cut(s) 1808, 2170
AspLEI GCGC 3 cut(s) 605, 634, 2052
AsuC2I CCSGG 2 cut(s) 534, 1016
AsuHPI GGTGA 5 cut(s) 290, 395, 814, 1598, 2533
AvaI CYCGRG 2 cut(s) 64, 729
AxyI CCTNAGG 1 cut(s) 2246
BalI TGGCCA 2 cut(s) 1436, 1443
BanII GRGCYC 2 cut(s) 534, 730
BauI CACGAG 4 cut(s) 89, 399, 524, 1539
BbsI GAAGAC 4 cut(s) 63, 947, 1672, 1687
Bbv12I GWGCWC 2 cut(s) 90, 730
BbvCI CCTCAGC 1 cut(s) 2394
BccI CCATC 7 cut(s) 32, 716, 1040, 1223, 1463, 1523, 2000
BciT130I CCWGG 2 cut(s) 826, 1626
BclI TGATCA 1 cut(s) 1792
BcnI CCSGG 2 cut(s) 534, 1016
BcoDI GTCTC 6 cut(s) 101, 201, 314, 368, 2246, 2439
BcuI ACTAGT 1 cut(s) 2291
BfaI CTAG 5 cut(s) 234, 558, 582, 2292, 2510
BfoI RGCGCY 1 cut(s) 2053
BfuAI ACCTGC 1 cut(s) 250
BlpI GCTNAGC 2 cut(s) 953, 2271
BmcAI AGTACT 1 cut(s) 1926
Bme1390I CCNGG 4 cut(s) 534, 826, 1016, 1626
BmeT110I CYCGRG 2 cut(s) 64, 729
BmiI GGNNCC 3 cut(s) 531, 1578, 1742
BmrFI CCNGG 4 cut(s) 534, 826, 1016, 1626
BmsI GCATC 4 cut(s) 543, 886, 933, 1523
BoxI GACNNNNGTC 1 cut(s) 1691
BpiI GAAGAC 4 cut(s) 63, 947, 1672, 1687
BplI GAGNNNNNCTC 2 cut(s) 197, 229
Bpu10I CCTNAGC 2 cut(s) 420, 2394
Bpu1102I GCTNAGC 2 cut(s) 953, 2271
BpuEI CTTGAG 2 cut(s) 346, 1566
BpuMI CCSGG 2 cut(s) 534, 1016
BsaBI GATNNNNATC 1 cut(s) 1188
BsaHI GRCGYC 2 cut(s) 1369, 2501
BsaI GGTCTC 2 cut(s) 314, 2246
BsaJI CCNNGG 2 cut(s) 825, 1571
BsaWI WCCGGW 3 cut(s) 1197, 1974, 2056
BsaXI ACNNNNNCTCC 6 cut(s) 77, 107, 717, 747, 2367, 2397
Bsc4I CCNNNNNNNGG 3 cut(s) 244, 2080, 2152
Bse118I RCCGGY 2 cut(s) 611, 1197
Bse1I ACTGG 3 cut(s) 429, 715, 2382
Bse21I CCTNAGG 1 cut(s) 2246
Bse3DI GCAATG 3 cut(s) 337, 605, 1480
Bse8I GATNNNNATC 1 cut(s) 1188
BseAI TCCGGA 2 cut(s) 1974, 2056
BseBI CCWGG 2 cut(s) 826, 1626
BseDI CCNNGG 2 cut(s) 825, 1571
BseGI GGATG 5 cut(s) 622, 877, 970, 1099, 1538
BseJI GATNNNNATC 1 cut(s) 1188
BseLI CCNNNNNNNGG 3 cut(s) 244, 2080, 2152
BseMI GCAATG 3 cut(s) 337, 605, 1480
BseMII CTCAG 6 cut(s) 847, 1698, 1942, 2262, 2385, 2394
BseNI ACTGG 3 cut(s) 429, 715, 2382
BseRI GAGGAG 2 cut(s) 332, 542
BseYI CCCAGC 1 cut(s) 1643
BsgI GTGCAG 2 cut(s) 333, 2498
BshFI GGCC 2 cut(s) 1436, 1443
BshTI ACCGGT 1 cut(s) 1197
BsiHKAI GWGCWC 2 cut(s) 90, 730
BsiHKCI CYCGRG 2 cut(s) 64, 729
BsiSI CCGG 6 cut(s) 534, 612, 1015, 1198, 1975, 2057
BslFI GGGAC 1 cut(s) 2512
BslI CCNNNNNNNGG 3 cut(s) 244, 2080, 2152
BsmAI GTCTC 6 cut(s) 101, 201, 314, 368, 2246, 2439
BsmBI CGTCTC 2 cut(s) 368, 2439
BsmFI GGGAC 1 cut(s) 2512
BsmI GAATGC 1 cut(s) 151
BsnI GGCC 2 cut(s) 1436, 1443
Bso31I GGTCTC 2 cut(s) 314, 2246
BsoBI CYCGRG 2 cut(s) 64, 729
Bsp1286I GDGCHC 3 cut(s) 90, 534, 730
Bsp13I TCCGGA 2 cut(s) 1974, 2056
Bsp143I GATC 4 cut(s) 170, 1189, 1792, 2142
Bsp1720I GCTNAGC 2 cut(s) 953, 2271
BspANI GGCC 2 cut(s) 1436, 1443
BspCNI CTCAG 6 cut(s) 846, 1699, 1941, 2263, 2386, 2395
BspEI TCCGGA 2 cut(s) 1974, 2056
BspLI GGNNCC 3 cut(s) 531, 1578, 1742
BspMI ACCTGC 1 cut(s) 250
BspPI GGATC 1 cut(s) 2137
BspTNI GGTCTC 2 cut(s) 314, 2246
BsrDI GCAATG 3 cut(s) 337, 605, 1480
BsrFI RCCGGY 2 cut(s) 611, 1197
BsrI ACTGG 3 cut(s) 429, 715, 2382
BssAI RCCGGY 2 cut(s) 611, 1197
BssECI CCNNGG 2 cut(s) 825, 1571
BssMI GATC 4 cut(s) 170, 1189, 1792, 2142
BssNI GRCGYC 2 cut(s) 1369, 2501
BssSI CACGAG 4 cut(s) 89, 399, 524, 1539
Bst2BI CACGAG 4 cut(s) 89, 399, 524, 1539
Bst2UI CCWGG 2 cut(s) 826, 1626
Bst4CI ACNGT 8 cut(s) 339, 1338, 1382, 1683, 1780, 1835, 1892, 2201
Bst6I CTCTTC 2 cut(s) 3, 999
BstACI GRCGYC 2 cut(s) 1369, 2501
BstC8I GCNNGC 3 cut(s) 426, 858, 1983
BstDSI CCRYGG 1 cut(s) 1571
BstF5I GGATG 5 cut(s) 622, 877, 970, 1099, 1538
BstH2I RGCGCY 1 cut(s) 2053
BstHHI GCGC 3 cut(s) 605, 634, 2052
BstKTI GATC 4 cut(s) 173, 1192, 1795, 2145
BstMAI GTCTC 6 cut(s) 101, 201, 314, 368, 2246, 2439
BstMBI GATC 4 cut(s) 170, 1189, 1792, 2142
BstMWI GCNNNNNNNGC 7 cut(s) 813, 992, 1241, 1358, 1470, 1734, 2399
BstNI CCWGG 2 cut(s) 826, 1626
BstNSI RCATGY 4 cut(s) 860, 1946, 1985, 2197
BstPAI GACNNNNGTC 1 cut(s) 1691
BstSCI CCNGG 4 cut(s) 532, 824, 1014, 1624
BstV2I GAAGAC 4 cut(s) 63, 947, 1672, 1687
BstXI CCANNNNNNTGG 1 cut(s) 1432
Bsu36I CCTNAGG 1 cut(s) 2246
BsuRI GGCC 2 cut(s) 1436, 1443
BtgI CCRYGG 1 cut(s) 1571
BtgZI GCGATG 1 cut(s) 566
BtsCI GGATG 5 cut(s) 622, 877, 970, 1099, 1538
BtsI GCAGTG 1 cut(s) 1471
BtsIMutI CAGTG 8 cut(s) 344, 841, 1471, 1759, 1802, 1840, 1936, 2044
BveI ACCTGC 1 cut(s) 250
Cac8I GCNNGC 3 cut(s) 426, 858, 1983
CfoI GCGC 3 cut(s) 605, 634, 2052
Cfr10I RCCGGY 2 cut(s) 611, 1197
CseI GACGC 2 cut(s) 1085, 1358
CsiI ACCWGGT 1 cut(s) 1624
Csp6I GTAC 6 cut(s) 342, 561, 830, 1144, 1925, 2197
CspAI ACCGGT 1 cut(s) 1197
CspCI CAANNNNNGTGG 1 cut(s) 38
CviQI GTAC 6 cut(s) 342, 561, 830, 1144, 1925, 2197
DpnI GATC 4 cut(s) 172, 1191, 1794, 2144
DpnII GATC 4 cut(s) 170, 1189, 1792, 2142
EaeI YGGCCR 2 cut(s) 1434, 1441
Eam1104I CTCTTC 2 cut(s) 3, 999
EarI CTCTTC 2 cut(s) 3, 999
Ecl136II GAGCTC 1 cut(s) 728
Eco24I GRGCYC 2 cut(s) 534, 730
Eco31I GGTCTC 2 cut(s) 314, 2246
Eco53kI GAGCTC 1 cut(s) 728
Eco57I CTGAAG 4 cut(s) 98, 959, 1077, 1182
Eco81I CCTNAGG 1 cut(s) 2246
Eco88I CYCGRG 2 cut(s) 64, 729
EcoICRI GAGCTC 1 cut(s) 728
EcoRI GAATTC 1 cut(s) 304
EcoRII CCWGG 2 cut(s) 824, 1624
EcoT22I ATGCAT 1 cut(s) 2342
EcoT38I GRGCYC 2 cut(s) 534, 730
Esp3I CGTCTC 2 cut(s) 368, 2439
FalI AAGNNNNNCTT 2 cut(s) 1017, 1049
FaqI GGGAC 1 cut(s) 2512
FauI CCCGC 4 cut(s) 75, 104, 759, 2395
FauNDI CATATG 1 cut(s) 841
FbaI TGATCA 1 cut(s) 1792
FblI GTMKAC 1 cut(s) 707
FokI GGATG 5 cut(s) 609, 864, 977, 1086, 1545
FriOI GRGCYC 2 cut(s) 534, 730
FspBI CTAG 5 cut(s) 234, 558, 582, 2292, 2510
GlaI GCGC 3 cut(s) 604, 633, 2051
GsaI CCCAGC 1 cut(s) 1647
HaeII RGCGCY 1 cut(s) 2053
HaeIII GGCC 2 cut(s) 1436, 1443
HapII CCGG 6 cut(s) 534, 612, 1015, 1198, 1975, 2057
HgaI GACGC 2 cut(s) 1085, 1358
HhaI GCGC 3 cut(s) 605, 634, 2052
Hin1I GRCGYC 2 cut(s) 1369, 2501
Hin6I GCGC 3 cut(s) 603, 632, 2050
HinP1I GCGC 3 cut(s) 603, 632, 2050
HincII GTYRAC 4 cut(s) 1373, 1686, 2484, 2529
HindII GTYRAC 4 cut(s) 1373, 1686, 2484, 2529
HindIII AAGCTT 3 cut(s) 422, 1082, 1117
HinfI GANTC 8 cut(s) 287, 442, 481, 871, 1007, 1649, 1900, 2405
HpaII CCGG 6 cut(s) 534, 612, 1015, 1198, 1975, 2057
HphI GGTGA 5 cut(s) 290, 395, 814, 1598, 2533
Hpy166II GTNNAC 8 cut(s) 342, 521, 708, 1373, 1499, 1686, 2484, 2529
Hpy188I TCNGA 5 cut(s) 175, 201, 486, 672, 1963
Hpy8I GTNNAC 8 cut(s) 342, 521, 708, 1373, 1499, 1686, 2484, 2529
HpyAV CCTTC 7 cut(s) 139, 693, 1021, 1157, 1341, 1798, 2080
HpyCH4III ACNGT 8 cut(s) 339, 1338, 1382, 1683, 1780, 1835, 1892, 2201
HpyCH4IV ACGT 3 cut(s) 455, 737, 2501
HpyF10VI GCNNNNNNNGC 7 cut(s) 813, 992, 1241, 1358, 1470, 1734, 2399
HpySE526I ACGT 3 cut(s) 455, 737, 2501
Hsp92I GRCGYC 2 cut(s) 1369, 2501
HspAI GCGC 3 cut(s) 603, 632, 2050
Kpn2I TCCGGA 2 cut(s) 1974, 2056
Ksp22I TGATCA 1 cut(s) 1792
Kzo9I GATC 4 cut(s) 170, 1189, 1792, 2142
LmnI GCTCC 9 cut(s) 85, 148, 218, 529, 725, 952, 1232, 1740, 1957
LweI GCATC 4 cut(s) 543, 886, 933, 1523
MabI ACCWGGT 1 cut(s) 1624
MaeI CTAG 5 cut(s) 234, 558, 582, 2292, 2510
MaeII ACGT 3 cut(s) 455, 737, 2501
MaeIII GTNAC 8 cut(s) 733, 1200, 1313, 1382, 1844, 1892, 2135, 2349
MalI GATC 4 cut(s) 172, 1191, 1794, 2144
MboI GATC 4 cut(s) 170, 1189, 1792, 2142
MboII GAAGA 9 cut(s) 20, 68, 113, 952, 1016, 1162, 1644, 1677, 1687
MfeI CAATTG 1 cut(s) 1455
MhlI GDGCHC 3 cut(s) 90, 534, 730
MlsI TGGCCA 2 cut(s) 1436, 1443
MluNI TGGCCA 2 cut(s) 1436, 1443
MlyI GAGTC 4 cut(s) 296, 865, 1016, 2414
MmeI TCCRAC 1 cut(s) 627
Mox20I TGGCCA 2 cut(s) 1436, 1443
Mph1103I ATGCAT 1 cut(s) 2342
MroI TCCGGA 2 cut(s) 1974, 2056
MroXI GAANNNNTTC 2 cut(s) 1808, 2170
MscI TGGCCA 2 cut(s) 1436, 1443
MseI TTAA 5 cut(s) 636, 693, 1035, 1850, 2210
MslI CAYNNNNRTG 4 cut(s) 156, 627, 861, 896
Msp20I TGGCCA 2 cut(s) 1436, 1443
MspA1I CMGCKG 1 cut(s) 2393
MspI CCGG 6 cut(s) 534, 612, 1015, 1198, 1975, 2057
MspR9I CCNGG 4 cut(s) 534, 826, 1016, 1626
MunI CAATTG 1 cut(s) 1455
Mva1269I GAATGC 1 cut(s) 151
MvaI CCWGG 2 cut(s) 826, 1626
MwoI GCNNNNNNNGC 7 cut(s) 813, 992, 1241, 1358, 1470, 1734, 2399
NciI CCSGG 2 cut(s) 534, 1016
NdeI CATATG 1 cut(s) 841
NdeII GATC 4 cut(s) 170, 1189, 1792, 2142
NlaIV GGNNCC 3 cut(s) 531, 1578, 1742
NmeAIII GCCGAG 1 cut(s) 853
NmuCI GTSAC 5 cut(s) 733, 1313, 1382, 1844, 2135
NsiI ATGCAT 1 cut(s) 2342
NspI RCATGY 4 cut(s) 860, 1946, 1985, 2197
PaeI GCATGC 2 cut(s) 860, 1985
PaeR7I CTCGAG 1 cut(s) 729
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PctI GAATGC 1 cut(s) 151
PdmI GAANNNNTTC 2 cut(s) 1808, 2170
PfeI GAWTC 4 cut(s) 442, 481, 1649, 1900
PinAI ACCGGT 1 cut(s) 1197
PleI GAGTC 4 cut(s) 295, 865, 1015, 2413
PpsI GAGTC 4 cut(s) 295, 865, 1015, 2413
PshAI GACNNNNGTC 1 cut(s) 1691
PsiI TTATAA 1 cut(s) 779
Psp124BI GAGCTC 1 cut(s) 730
Psp6I CCWGG 2 cut(s) 824, 1624
PspFI CCCAGC 1 cut(s) 1643
PspGI CCWGG 2 cut(s) 824, 1624
PspN4I GGNNCC 3 cut(s) 531, 1578, 1742
PspXI VCTCGAGB 1 cut(s) 729
PvuII CAGCTG 1 cut(s) 2393
RsaI GTAC 6 cut(s) 343, 562, 831, 1145, 1926, 2198
RsaNI GTAC 6 cut(s) 342, 561, 830, 1144, 1925, 2197
RseI CAYNNNNRTG 4 cut(s) 156, 627, 861, 896
SacI GAGCTC 1 cut(s) 730
SaqAI TTAA 5 cut(s) 636, 693, 1035, 1850, 2210
Sau3AI GATC 4 cut(s) 170, 1189, 1792, 2142
ScaI AGTACT 1 cut(s) 1926
SchI GAGTC 4 cut(s) 296, 865, 1016, 2414
ScrFI CCNGG 4 cut(s) 534, 826, 1016, 1626
SduI GDGCHC 3 cut(s) 90, 534, 730
SexAI ACCWGGT 1 cut(s) 1624
SfaNI GCATC 4 cut(s) 543, 886, 933, 1523
Sfr274I CTCGAG 1 cut(s) 729
SlaI CTCGAG 1 cut(s) 729
SmiMI CAYNNNNRTG 4 cut(s) 156, 627, 861, 896
SmlI CTYRAG 3 cut(s) 325, 729, 1581
SmoI CTYRAG 3 cut(s) 325, 729, 1581
SpeI ACTAGT 1 cut(s) 2291
SphI GCATGC 2 cut(s) 860, 1985
SspI AATATT 1 cut(s) 2170
SspMI CTAG 5 cut(s) 234, 558, 582, 2292, 2510
SstI GAGCTC 1 cut(s) 730
StyD4I CCNGG 4 cut(s) 532, 824, 1014, 1624
TaaI ACNGT 8 cut(s) 339, 1338, 1382, 1683, 1780, 1835, 1892, 2201
TaiI ACGT 3 cut(s) 458, 740, 2504
TaqI TCGA 5 cut(s) 99, 730, 1039, 2098, 2459
TatI WGTACW 3 cut(s) 560, 1924, 2196
TauI GCSGC 2 cut(s) 804, 1760
TfiI GAWTC 4 cut(s) 442, 481, 1649, 1900
Tru1I TTAA 5 cut(s) 636, 693, 1035, 1850, 2210
Tru9I TTAA 5 cut(s) 636, 693, 1035, 1850, 2210
TscAI CASTG 8 cut(s) 344, 841, 1471, 1759, 1802, 1840, 1936, 2044
TseFI GTSAC 5 cut(s) 733, 1313, 1382, 1844, 2135
Tsp45I GTSAC 5 cut(s) 733, 1313, 1382, 1844, 2135
TspDTI ATGAA 9 cut(s) 423, 576, 611, 981, 1017, 1728, 1785, 1892, 2325
TspGWI ACGGA 1 cut(s) 1119
TspRI CASTG 8 cut(s) 344, 841, 1471, 1759, 1802, 1840, 1936, 2044
XapI RAATTY 4 cut(s) 304, 414, 1657, 1918
XceI RCATGY 4 cut(s) 860, 1946, 1985, 2197
XhoI CTCGAG 1 cut(s) 729
XmiI GTMKAC 1 cut(s) 707
XmnI GAANNNNTTC 2 cut(s) 1808, 2170
XspI CTAG 5 cut(s) 234, 558, 582, 2292, 2510
ZraI GACGTC 1 cut(s) 2502
ZrmI AGTACT 1 cut(s) 1926
Zsp2I ATGCAT 1 cut(s) 2342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.