MD12G1140600.v1.1

cyclin-dependent protein kinase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0015384
Physical Location & Seq
Reverse (-)
21858132 .. 21862216
4085 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1140600.v1.1.491

Sequence Viewer

Length: 525 bp
ATGGAGTCAAACCTAGTCGACCGGATCTCCGTCCTCAACGTCTCGGATTCACTCAAACAGGACTGGAGCGAGAAACCCACGTCGGAGTTTCAATCCACGCCATCGGATCACGATCATGCGCCTCATGATCATCAACAACAAGAACAGCCCAAGAAACAAGAAACAACTGAGCAGTCCCAGAACATTACACCAGATCACATTAACACCCCGGCAAAACAAGACCGCAAGAAGCCGCCGTTGCCGGAGGAAGATGAATCGTCGACGAACGACAAGGACGTCATGGATGAGGACAACGACGGGTTCAAAACGCCGACGTCTCTGGAACACAAAATCCCGCCGACGACAGTTTGTCCGCCTGCGCCGCGAAAACCCTTTCTAAAGAGAAGGCGGTCGCCGTCGCCTCCGCCCCCGGCTGCTCGCAGAAAGGTTCAGTTTACTCAAGCACAACTGGATGATATTTTCTCCAACTTTTTACTCATCCCGGATGCGCGCGCCAAAATCAAGAAAGCCAGACGATCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

175

Amino Acids

19.86

Weight (kDa)

6.32

Isoelectric Point (pI)

80.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015384)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28870 AT5G02420
fragaria_vesca FvH4_6g13950
malus_domestica MD12G1140600.v1.1
prunus_persica Prupe.6G254000_v2.0.a1
pyrus_communis pycom04g11420 pycom12g13560
rosa_chinensis RchiOBHm_Chr3g0466771
rosa_laevigata RLG00000024554
rosa_roxburghii Rroxscaffold_6G00414190
rosa_rugosa Rorug03G0083800
rosa_samantha Rh3AG130900 Rh3BG151900 Rh3CG151800 Rh3DG152600
rosa_wichuraiana Rw3G012370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 275
AatII GACGTC 2 cut(s) 279, 317
AccI GTMKAC 2 cut(s) 18, 260
AccII CGCG 3 cut(s) 364, 490, 492
AciI CCGC 7 cut(s) 223, 233, 335, 353, 362, 388, 404
AclWI GGATC 2 cut(s) 32, 114
AcyI GRCGYC 2 cut(s) 276, 314
AgsI TTSAA 2 cut(s) 92, 304
AhdI GACNNNNNGTC 1 cut(s) 348
AjiI CACGTC 1 cut(s) 81
AloI GAACNNNNNNTCC 2 cut(s) 315, 347
Alw26I GTCTC 2 cut(s) 46, 321
AlwI GGATC 2 cut(s) 32, 114
ApeKI GCWGC 1 cut(s) 413
AspLEI GCGC 5 cut(s) 121, 361, 490, 492, 494
AsuC2I CCSGG 3 cut(s) 209, 410, 482
BbvI GCAGC 1 cut(s) 400
BccI CCATC 1 cut(s) 109
BceAI ACGGC 2 cut(s) 220, 379
BclI TGATCA 1 cut(s) 127
BcnI CCSGG 3 cut(s) 209, 410, 482
BcoDI GTCTC 2 cut(s) 46, 321
BfaI CTAG 1 cut(s) 14
BisI GCNGC 3 cut(s) 233, 362, 414
BlsI GCNGC 3 cut(s) 234, 363, 415
Bme1390I CCNGG 3 cut(s) 209, 410, 482
BmeRI GACNNNNNGTC 1 cut(s) 348
BmgBI CACGTC 1 cut(s) 81
BmrFI CCNGG 3 cut(s) 209, 410, 482
BmsI GCATC 1 cut(s) 475
BpmI CTGGAG 1 cut(s) 85
BpuEI CTTGAG 1 cut(s) 423
BpuMI CCSGG 3 cut(s) 209, 410, 482
BsaBI GATNNNNATC 1 cut(s) 111
BsaHI GRCGYC 2 cut(s) 276, 314
BsaJI CCNNGG 2 cut(s) 207, 408
BsaWI WCCGGW 1 cut(s) 21
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bse1I ACTGG 2 cut(s) 68, 453
Bse8I GATNNNNATC 1 cut(s) 111
BseDI CCNNGG 2 cut(s) 207, 408
BseGI GGATG 4 cut(s) 289, 457, 477, 490
BseJI GATNNNNATC 1 cut(s) 111
BseMII CTCAG 1 cut(s) 159
BseNI ACTGG 2 cut(s) 68, 453
BsePI GCGCGC 2 cut(s) 488, 490
BseXI GCAGC 1 cut(s) 400
Bsh1236I CGCG 3 cut(s) 364, 490, 492
Bsh1285I CGRYCG 2 cut(s) 22, 392
BsiEI CGRYCG 2 cut(s) 22, 392
BsiSI CCGG 5 cut(s) 22, 209, 242, 410, 482
BslFI GGGAC 1 cut(s) 160
BsmAI GTCTC 2 cut(s) 46, 321
BsmBI CGTCTC 2 cut(s) 46, 321
BsmFI GGGAC 1 cut(s) 160
Bsp143I GATC 6 cut(s) 24, 106, 112, 127, 193, 515
BspACI CCGC 7 cut(s) 223, 233, 335, 353, 362, 388, 404
BspCNI CTCAG 1 cut(s) 160
BspFNI CGCG 3 cut(s) 364, 490, 492
BspHI TCATGA 1 cut(s) 124
BspPI GGATC 2 cut(s) 32, 114
BsrI ACTGG 2 cut(s) 68, 453
BssECI CCNNGG 2 cut(s) 207, 408
BssHII GCGCGC 2 cut(s) 488, 490
BssMI GATC 6 cut(s) 24, 106, 112, 127, 193, 515
BssNI GRCGYC 2 cut(s) 276, 314
Bst4CI ACNGT 1 cut(s) 346
BstACI GRCGYC 2 cut(s) 276, 314
BstC8I GCNNGC 4 cut(s) 357, 418, 490, 492
BstDEI CTNAG 1 cut(s) 168
BstF5I GGATG 4 cut(s) 289, 457, 477, 490
BstFNI CGCG 3 cut(s) 364, 490, 492
BstHHI GCGC 5 cut(s) 121, 361, 490, 492, 494
BstKTI GATC 6 cut(s) 27, 109, 115, 130, 196, 518
BstMAI GTCTC 2 cut(s) 46, 321
BstMBI GATC 6 cut(s) 24, 106, 112, 127, 193, 515
BstMCI CGRYCG 2 cut(s) 22, 392
BstMWI GCNNNNNNNGC 2 cut(s) 238, 361
BstSCI CCNGG 3 cut(s) 207, 408, 480
BstUI CGCG 3 cut(s) 364, 490, 492
BstV1I GCAGC 1 cut(s) 400
BstX2I RGATCY 1 cut(s) 24
BstYI RGATCY 1 cut(s) 24
BtrI CACGTC 1 cut(s) 81
BtsCI GGATG 4 cut(s) 289, 457, 477, 490
Cac8I GCNNGC 4 cut(s) 357, 418, 490, 492
CciI TCATGA 1 cut(s) 124
CfoI GCGC 5 cut(s) 121, 361, 490, 492, 494
CviAII CATG 3 cut(s) 116, 125, 280
CviJI RGCY 4 cut(s) 148, 232, 413, 509
CviKI_1 RGCY 4 cut(s) 148, 232, 413, 509
DdeI CTNAG 1 cut(s) 168
DpnI GATC 6 cut(s) 26, 108, 114, 129, 195, 517
DpnII GATC 6 cut(s) 24, 106, 112, 127, 193, 515
DrdI GACNNNNNNGTC 1 cut(s) 275
DriI GACNNNNNGTC 1 cut(s) 348
DseDI GACNNNNNNGTC 1 cut(s) 275
Eam1105I GACNNNNNGTC 1 cut(s) 348
EciI GGCGGA 2 cut(s) 342, 393
Esp3I CGTCTC 2 cut(s) 46, 321
FaeI CATG 3 cut(s) 119, 128, 283
FaiI YATR 3 cut(s) 117, 126, 281
FaqI GGGAC 1 cut(s) 160
FatI CATG 3 cut(s) 115, 124, 279
FauI CCCGC 1 cut(s) 342
FbaI TGATCA 1 cut(s) 127
FblI GTMKAC 2 cut(s) 18, 260
Fnu4HI GCNGC 3 cut(s) 233, 362, 414
FokI GGATG 4 cut(s) 296, 464, 464, 497
Fsp4HI GCNGC 3 cut(s) 233, 362, 414
FspBI CTAG 1 cut(s) 14
GlaI GCGC 5 cut(s) 120, 360, 489, 491, 493
GluI GCNGC 3 cut(s) 233, 362, 414
GsuI CTGGAG 1 cut(s) 85
HapII CCGG 5 cut(s) 22, 209, 242, 410, 482
HhaI GCGC 5 cut(s) 121, 361, 490, 492, 494
Hin1I GRCGYC 2 cut(s) 276, 314
Hin1II CATG 3 cut(s) 119, 128, 283
Hin6I GCGC 5 cut(s) 119, 359, 488, 490, 492
HinP1I GCGC 5 cut(s) 119, 359, 488, 490, 492
HincII GTYRAC 2 cut(s) 19, 261
HindII GTYRAC 2 cut(s) 19, 261
HinfI GANTC 3 cut(s) 5, 47, 254
HpaII CCGG 5 cut(s) 22, 209, 242, 410, 482
Hpy166II GTNNAC 3 cut(s) 19, 261, 435
Hpy188I TCNGA 4 cut(s) 46, 85, 106, 520
Hpy188III TCNNGA 4 cut(s) 110, 125, 320, 502
Hpy8I GTNNAC 3 cut(s) 19, 261, 435
Hpy99I CGWCG 7 cut(s) 85, 262, 265, 299, 316, 343, 400
HpyAV CCTTC 1 cut(s) 378
HpyCH4III ACNGT 1 cut(s) 346
HpyCH4IV ACGT 4 cut(s) 39, 80, 276, 314
HpyF10VI GCNNNNNNNGC 2 cut(s) 238, 361
HpyF3I CTNAG 1 cut(s) 168
HpySE526I ACGT 4 cut(s) 39, 80, 276, 314
Hsp92I GRCGYC 2 cut(s) 276, 314
Hsp92II CATG 3 cut(s) 119, 128, 283
HspAI GCGC 5 cut(s) 119, 359, 488, 490, 492
Ksp22I TGATCA 1 cut(s) 127
Kzo9I GATC 6 cut(s) 24, 106, 112, 127, 193, 515
LmnI GCTCC 1 cut(s) 66
Lsp1109I GCAGC 1 cut(s) 400
LweI GCATC 1 cut(s) 475
MaeI CTAG 1 cut(s) 14
MaeII ACGT 4 cut(s) 39, 80, 276, 314
MalI GATC 6 cut(s) 26, 108, 114, 129, 195, 517
MboI GATC 6 cut(s) 24, 106, 112, 127, 193, 515
MboII GAAGA 1 cut(s) 260
MflI RGATCY 1 cut(s) 24
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 2 cut(s) 63, 489
MnlI CCTC 5 cut(s) 44, 132, 238, 280, 411
MseI TTAA 1 cut(s) 201
MslI CAYNNNNRTG 1 cut(s) 114
MspI CCGG 5 cut(s) 22, 209, 242, 410, 482
MspR9I CCNGG 3 cut(s) 209, 410, 482
MvnI CGCG 3 cut(s) 364, 490, 492
MwoI GCNNNNNNNGC 2 cut(s) 238, 361
NciI CCSGG 3 cut(s) 209, 410, 482
NdeII GATC 6 cut(s) 24, 106, 112, 127, 193, 515
NlaIII CATG 3 cut(s) 119, 128, 283
PagI TCATGA 1 cut(s) 124
PauI GCGCGC 2 cut(s) 488, 490
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PfeI GAWTC 2 cut(s) 47, 254
PfoI TCCNGGA 1 cut(s) 480
PkrI GCNGC 3 cut(s) 234, 363, 415
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PsuI RGATCY 1 cut(s) 24
PteI GCGCGC 2 cut(s) 488, 490
RseI CAYNNNNRTG 1 cut(s) 114
SalI GTCGAC 2 cut(s) 17, 259
SaqAI TTAA 1 cut(s) 201
SatI GCNGC 3 cut(s) 233, 362, 414
Sau3AI GATC 6 cut(s) 24, 106, 112, 127, 193, 515
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 3 cut(s) 209, 410, 482
SetI ASST 6 cut(s) 15, 42, 83, 279, 317, 429
SfaNI GCATC 1 cut(s) 475
SgrDI CGTCGACG 1 cut(s) 259
SmiMI CAYNNNNRTG 1 cut(s) 114
SmlI CTYRAG 1 cut(s) 438
SmoI CTYRAG 1 cut(s) 438
SsiI CCGC 7 cut(s) 223, 233, 335, 353, 362, 388, 404
SspMI CTAG 1 cut(s) 14
StyD4I CCNGG 3 cut(s) 207, 408, 480
TaaI ACNGT 1 cut(s) 346
TaiI ACGT 4 cut(s) 42, 83, 279, 317
TaqI TCGA 2 cut(s) 18, 260
TauI GCSGC 2 cut(s) 235, 364
TfiI GAWTC 2 cut(s) 47, 254
Tru1I TTAA 1 cut(s) 201
Tru9I TTAA 1 cut(s) 201
TseI GCWGC 1 cut(s) 413
TspDTI ATGAA 1 cut(s) 267
TspGWI ACGGA 1 cut(s) 19
XmiI GTMKAC 2 cut(s) 18, 260
XspI CTAG 1 cut(s) 14
ZraI GACGTC 2 cut(s) 277, 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.