pycom04g11420

cyclin-dependent protein kinase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0015384
Physical Location & Seq
Reverse (-)
14286423 .. 14286965
543 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g11420.1

Sequence Viewer

Length: 543 bp
ATGGAGTCGAACCTAGTCGACCGGATCTCCGTCCTCAACGTCTCAAATTCACACAAACAAGACCGGAGCGAGACACCCATGTCGGAGTTTCAATCCAAGCCGTCGGATCACGATGATGCGCCTCACGATCGTCAACAACAAGAACAGCCCAAGAAACAAGAACCAGAGCAGTCCCAGAACATTACACCAGATCACATTAACACCCCGGCAAAACAAGACCGCAAGACGCCGCCGTCCCCGATGGAAGATGAATCATCGACGAACGACAAGGACGTCGTGGATGACGACGACGATGGGTTCAAAACGCCAACGTCCCTGGAGCACAAAATCCCGCCGATGACAGAATGCCCGCCTGCGCCGCGCAAACCCAAACGCTTTCTAAAGAGAAAAATGTCGTCGCGGTCGCCGTCGCCTCCGCCGCCGCCCGCTCGCAGAAAGGTTCAGTTTACAACAGCACAACTGGATATGATATTTTGGTCAACTTTTCCCTCGCCTCGGATGGGCGCGCCAAAATCAAGAAAGCCAGACGATCAGAGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

20.49

Weight (kDa)

6.22

Isoelectric Point (pI)

91.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015384)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28870 AT5G02420
fragaria_vesca FvH4_6g13950
malus_domestica MD12G1140600.v1.1
prunus_persica Prupe.6G254000_v2.0.a1
pyrus_communis pycom04g11420 pycom12g13560
rosa_chinensis RchiOBHm_Chr3g0466771
rosa_laevigata RLG00000024554
rosa_roxburghii Rroxscaffold_6G00414190
rosa_rugosa Rorug03G0083800
rosa_samantha Rh3AG130900 Rh3BG151900 Rh3CG151800 Rh3DG152600
rosa_wichuraiana Rw3G012370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 79, 232, 272
AatII GACGTC 1 cut(s) 276
AccBSI CCGCTC 1 cut(s) 428
AccI GTMKAC 1 cut(s) 18
AccII CGCG 3 cut(s) 361, 400, 506
AclWI GGATC 2 cut(s) 32, 114
AcsI RAATTY 1 cut(s) 46
AcyI GRCGYC 2 cut(s) 227, 273
AfiI CCNNNNNNNGG 2 cut(s) 495, 500
AgsI TTSAA 2 cut(s) 92, 301
AjnI CCWGG 1 cut(s) 315
Alw21I GWGCWC 1 cut(s) 324
Alw26I GTCTC 2 cut(s) 46, 65
AlwI GGATC 2 cut(s) 32, 114
ApoI RAATTY 1 cut(s) 46
AscI GGCGCGCC 1 cut(s) 504
AspLEI GCGC 5 cut(s) 121, 358, 363, 506, 508
AsuC2I CCSGG 1 cut(s) 206
Bbv12I GWGCWC 1 cut(s) 324
BccI CCATC 3 cut(s) 235, 287, 493
BceAI ACGGC 3 cut(s) 85, 217, 391
BciT130I CCWGG 1 cut(s) 317
BcnI CCSGG 1 cut(s) 206
BcoDI GTCTC 2 cut(s) 46, 65
BfaI CTAG 1 cut(s) 14
BisI GCNGC 4 cut(s) 230, 359, 419, 422
BlsI GCNGC 4 cut(s) 231, 360, 420, 423
Bme1390I CCNGG 2 cut(s) 206, 317
BmrFI CCNGG 2 cut(s) 206, 317
BmsI GCATC 1 cut(s) 106
BpmI CTGGAG 1 cut(s) 338
BpuMI CCSGG 1 cut(s) 206
BsaHI GRCGYC 2 cut(s) 227, 273
BsaJI CCNNGG 3 cut(s) 204, 315, 494
BsaWI WCCGGW 2 cut(s) 21, 63
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 2 cut(s) 495, 500
Bse1I ACTGG 1 cut(s) 465
BseBI CCWGG 1 cut(s) 317
BseDI CCNNGG 3 cut(s) 204, 315, 494
BseGI GGATG 2 cut(s) 286, 504
BseLI CCNNNNNNNGG 2 cut(s) 495, 500
BseNI ACTGG 1 cut(s) 465
BsePI GCGCGC 1 cut(s) 504
Bsh1236I CGCG 3 cut(s) 361, 400, 506
Bsh1285I CGRYCG 3 cut(s) 22, 130, 404
BsiEI CGRYCG 3 cut(s) 22, 130, 404
BsiHKAI GWGCWC 1 cut(s) 324
BsiSI CCGG 3 cut(s) 22, 64, 206
BslFI GGGAC 3 cut(s) 157, 220, 298
BslI CCNNNNNNNGG 2 cut(s) 495, 500
BsmAI GTCTC 2 cut(s) 46, 65
BsmBI CGTCTC 1 cut(s) 46
BsmFI GGGAC 3 cut(s) 157, 220, 298
BsmI GAATGC 1 cut(s) 350
Bsp1286I GDGCHC 1 cut(s) 324
Bsp143I GATC 5 cut(s) 24, 106, 127, 190, 529
BspFNI CGCG 3 cut(s) 361, 400, 506
BspPI GGATC 2 cut(s) 32, 114
BsrBI CCGCTC 1 cut(s) 428
BsrI ACTGG 1 cut(s) 465
BssECI CCNNGG 3 cut(s) 204, 315, 494
BssHII GCGCGC 1 cut(s) 504
BssMI GATC 5 cut(s) 24, 106, 127, 190, 529
BssNI GRCGYC 2 cut(s) 227, 273
Bst2UI CCWGG 1 cut(s) 317
BstACI GRCGYC 2 cut(s) 227, 273
BstC8I GCNNGC 5 cut(s) 350, 354, 426, 430, 506
BstF5I GGATG 2 cut(s) 286, 504
BstFNI CGCG 3 cut(s) 361, 400, 506
BstHHI GCGC 5 cut(s) 121, 358, 363, 506, 508
BstKTI GATC 5 cut(s) 27, 109, 130, 193, 532
BstMAI GTCTC 2 cut(s) 46, 65
BstMBI GATC 5 cut(s) 24, 106, 127, 190, 529
BstMCI CGRYCG 3 cut(s) 22, 130, 404
BstMWI GCNNNNNNNGC 2 cut(s) 358, 418
BstNI CCWGG 1 cut(s) 317
BstSCI CCNGG 2 cut(s) 204, 315
BstUI CGCG 3 cut(s) 361, 400, 506
BstX2I RGATCY 1 cut(s) 24
BstYI RGATCY 1 cut(s) 24
BtsCI GGATG 2 cut(s) 286, 504
Cac8I GCNNGC 5 cut(s) 350, 354, 426, 430, 506
CfoI GCGC 5 cut(s) 121, 358, 363, 506, 508
CseI GACGC 1 cut(s) 235
CviAII CATG 1 cut(s) 79
CviJI RGCY 3 cut(s) 100, 148, 523
CviKI_1 RGCY 3 cut(s) 100, 148, 523
DpnI GATC 5 cut(s) 26, 108, 129, 192, 531
DpnII GATC 5 cut(s) 24, 106, 127, 190, 529
DrdI GACNNNNNNGTC 3 cut(s) 79, 232, 272
DseDI GACNNNNNNGTC 3 cut(s) 79, 232, 272
EciI GGCGGA 1 cut(s) 405
EcoRII CCWGG 1 cut(s) 315
Esp3I CGTCTC 1 cut(s) 46
FaeI CATG 1 cut(s) 82
FaiI YATR 2 cut(s) 80, 467
FaqI GGGAC 3 cut(s) 157, 220, 298
FatI CATG 1 cut(s) 78
FauI CCCGC 3 cut(s) 339, 357, 433
FblI GTMKAC 1 cut(s) 18
Fnu4HI GCNGC 4 cut(s) 230, 359, 419, 422
FokI GGATG 2 cut(s) 293, 511
Fsp4HI GCNGC 4 cut(s) 230, 359, 419, 422
FspBI CTAG 1 cut(s) 14
GlaI GCGC 5 cut(s) 120, 357, 362, 505, 507
GluI GCNGC 4 cut(s) 230, 359, 419, 422
GsuI CTGGAG 1 cut(s) 338
HapII CCGG 3 cut(s) 22, 64, 206
HgaI GACGC 1 cut(s) 235
HhaI GCGC 5 cut(s) 121, 358, 363, 506, 508
Hin1I GRCGYC 2 cut(s) 227, 273
Hin1II CATG 1 cut(s) 82
Hin6I GCGC 5 cut(s) 119, 356, 361, 504, 506
HinP1I GCGC 5 cut(s) 119, 356, 361, 504, 506
HincII GTYRAC 3 cut(s) 19, 134, 480
HindII GTYRAC 3 cut(s) 19, 134, 480
HinfI GANTC 2 cut(s) 5, 251
HpaII CCGG 3 cut(s) 22, 64, 206
Hpy166II GTNNAC 4 cut(s) 19, 134, 447, 480
Hpy188I TCNGA 4 cut(s) 85, 106, 498, 534
Hpy188III TCNNGA 3 cut(s) 110, 125, 516
Hpy8I GTNNAC 4 cut(s) 19, 134, 447, 480
Hpy99I CGWCG 7 cut(s) 106, 262, 278, 290, 293, 400, 412
HpyCH4IV ACGT 3 cut(s) 39, 273, 311
HpyF10VI GCNNNNNNNGC 2 cut(s) 358, 418
HpySE526I ACGT 3 cut(s) 39, 273, 311
Hsp92I GRCGYC 2 cut(s) 227, 273
Hsp92II CATG 1 cut(s) 82
HspAI GCGC 5 cut(s) 119, 356, 361, 504, 506
Kzo9I GATC 5 cut(s) 24, 106, 127, 190, 529
LmnI GCTCC 2 cut(s) 66, 319
LweI GCATC 1 cut(s) 106
MaeI CTAG 1 cut(s) 14
MaeII ACGT 3 cut(s) 39, 273, 311
MalI GATC 5 cut(s) 26, 108, 129, 192, 531
MbiI CCGCTC 1 cut(s) 428
MboI GATC 5 cut(s) 24, 106, 127, 190, 529
MboII GAAGA 1 cut(s) 257
MflI RGATCY 1 cut(s) 24
MhlI GDGCHC 1 cut(s) 324
MluCI AATT 1 cut(s) 46
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 2 cut(s) 63, 84
MnlI CCTC 5 cut(s) 44, 132, 423, 499, 504
MseI TTAA 1 cut(s) 198
MslI CAYNNNNRTG 1 cut(s) 114
MspI CCGG 3 cut(s) 22, 64, 206
MspR9I CCNGG 2 cut(s) 206, 317
MteI GCGCNGCGC 1 cut(s) 359
Mva1269I GAATGC 1 cut(s) 350
MvaI CCWGG 1 cut(s) 317
MvnI CGCG 3 cut(s) 361, 400, 506
MwoI GCNNNNNNNGC 2 cut(s) 358, 418
NciI CCSGG 1 cut(s) 206
NdeII GATC 5 cut(s) 24, 106, 127, 190, 529
NlaIII CATG 1 cut(s) 82
PalAI GGCGCGCC 1 cut(s) 504
PauI GCGCGC 1 cut(s) 504
PcsI WCGNNNNNNNCGW 3 cut(s) 270, 282, 404
PctI GAATGC 1 cut(s) 350
PfeI GAWTC 1 cut(s) 251
PkrI GCNGC 4 cut(s) 231, 360, 420, 423
Ple19I CGATCG 1 cut(s) 130
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Psp6I CCWGG 1 cut(s) 315
PspGI CCWGG 1 cut(s) 315
PsuI RGATCY 1 cut(s) 24
PteI GCGCGC 1 cut(s) 504
PvuI CGATCG 1 cut(s) 130
RseI CAYNNNNRTG 1 cut(s) 114
SalI GTCGAC 1 cut(s) 17
SaqAI TTAA 1 cut(s) 198
SatI GCNGC 4 cut(s) 230, 359, 419, 422
Sau3AI GATC 5 cut(s) 24, 106, 127, 190, 529
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 2 cut(s) 206, 317
SduI GDGCHC 1 cut(s) 324
SetI ASST 5 cut(s) 15, 42, 276, 314, 441
SfaNI GCATC 1 cut(s) 106
SgsI GGCGCGCC 1 cut(s) 504
SmiMI CAYNNNNRTG 1 cut(s) 114
Sse9I AATT 1 cut(s) 46
SspMI CTAG 1 cut(s) 14
StyD4I CCNGG 2 cut(s) 204, 315
TaiI ACGT 3 cut(s) 42, 276, 314
TaqI TCGA 3 cut(s) 8, 18, 257
TasI AATT 1 cut(s) 46
TauI GCSGC 4 cut(s) 232, 361, 421, 424
TfiI GAWTC 1 cut(s) 251
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TspDTI ATGAA 1 cut(s) 264
TspGWI ACGGA 1 cut(s) 19
XapI RAATTY 1 cut(s) 46
XmiI GTMKAC 1 cut(s) 18
XspI CTAG 1 cut(s) 14
ZraI GACGTC 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.