pycom12g13560

cyclin-dependent protein kinase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0015384
Physical Location & Seq
Reverse (-)
16052983 .. 16053504
522 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g13560.1

Sequence Viewer

Length: 522 bp
ATGGAGTCAAACCTAGTCGACCGGATCTCCGTCCTCAACGTCTCGAATTCACTCAAACAAGACCGGAGCGAGAAACCCATGTCAGAGTTTCGATCCACACCGTCGGATCACGATCATGCGCCTCATGATCATCAACAACAAGAACAGTCCAAGAAACAACAAACAAAGCAGTCCCAGAACATTACACCAGATCACATTAACACCCCGGCAAAACAAGACCGCAAGAAGCCGCCGCTGCCGGGGGAAGATGAATCGTCGACGAACGACAAGGATGTCATGGATGAGGACGACGACGGGTTCAAAACGCCGACGTCTCTGGAGCACAAAATCCCGCCGATGACAGAATGTCCGCCAGCGCCGCGAAAACCCTTTCTAAAGAGAAGGCGGTCGCCGTCGCCTCCGCCGCCGGCTGCTCGCAGAAAGGTTCAGTTTACTCAAGCACAACTGAATGATATTTTCTCCAACTTTTTACTCGTCCCGGATGCGCGCGCCAAAATCAAGAAAGCCAGACGATCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

174

Amino Acids

19.75

Weight (kDa)

9.21

Isoelectric Point (pI)

85.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015384)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28870 AT5G02420
fragaria_vesca FvH4_6g13950
malus_domestica MD12G1140600.v1.1
prunus_persica Prupe.6G254000_v2.0.a1
pyrus_communis pycom04g11420 pycom12g13560
rosa_chinensis RchiOBHm_Chr3g0466771
rosa_laevigata RLG00000024554
rosa_roxburghii Rroxscaffold_6G00414190
rosa_rugosa Rorug03G0083800
rosa_samantha Rh3AG130900 Rh3BG151900 Rh3CG151800 Rh3DG152600
rosa_wichuraiana Rw3G012370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 272
AatII GACGTC 1 cut(s) 314
AccI GTMKAC 2 cut(s) 18, 257
AccII CGCG 3 cut(s) 361, 487, 489
AciI CCGC 9 cut(s) 220, 230, 233, 332, 350, 359, 385, 401, 404
AclWI GGATC 3 cut(s) 32, 87, 114
AcsI RAATTY 1 cut(s) 46
AcyI GRCGYC 1 cut(s) 311
AfiI CCNNNNNNNGG 1 cut(s) 239
AgsI TTSAA 1 cut(s) 301
AhdI GACNNNNNGTC 1 cut(s) 345
Alw21I GWGCWC 1 cut(s) 324
Alw26I GTCTC 2 cut(s) 46, 318
AlwI GGATC 3 cut(s) 32, 87, 114
ApeKI GCWGC 2 cut(s) 235, 410
ApoI RAATTY 1 cut(s) 46
AspLEI GCGC 5 cut(s) 121, 358, 487, 489, 491
AsuC2I CCSGG 3 cut(s) 206, 240, 479
Bbv12I GWGCWC 1 cut(s) 324
BbvI GCAGC 2 cut(s) 222, 397
BceAI ACGGC 1 cut(s) 376
BclI TGATCA 1 cut(s) 127
BcnI CCSGG 3 cut(s) 206, 240, 479
BcoDI GTCTC 2 cut(s) 46, 318
BfaI CTAG 1 cut(s) 14
BfoI RGCGCY 1 cut(s) 359
BisI GCNGC 6 cut(s) 230, 233, 236, 359, 404, 411
BlsI GCNGC 6 cut(s) 231, 234, 237, 360, 405, 412
Bme1390I CCNGG 3 cut(s) 206, 240, 479
BmeRI GACNNNNNGTC 1 cut(s) 345
BmrFI CCNGG 3 cut(s) 206, 240, 479
BmsI GCATC 1 cut(s) 472
BpmI CTGGAG 1 cut(s) 338
BpuEI CTTGAG 1 cut(s) 420
BpuMI CCSGG 3 cut(s) 206, 240, 479
BsaBI GATNNNNATC 1 cut(s) 111
BsaHI GRCGYC 1 cut(s) 311
BsaJI CCNNGG 2 cut(s) 204, 239
BsaWI WCCGGW 2 cut(s) 21, 63
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 1 cut(s) 239
Bse118I RCCGGY 1 cut(s) 406
Bse8I GATNNNNATC 1 cut(s) 111
BseDI CCNNGG 2 cut(s) 204, 239
BseGI GGATG 3 cut(s) 277, 286, 487
BseJI GATNNNNATC 1 cut(s) 111
BseLI CCNNNNNNNGG 1 cut(s) 239
BsePI GCGCGC 2 cut(s) 485, 487
BseXI GCAGC 2 cut(s) 222, 397
Bsh1236I CGCG 3 cut(s) 361, 487, 489
Bsh1285I CGRYCG 2 cut(s) 22, 389
BsiEI CGRYCG 2 cut(s) 22, 389
BsiHKAI GWGCWC 1 cut(s) 324
BsiSI CCGG 6 cut(s) 22, 64, 206, 239, 407, 479
BslFI GGGAC 2 cut(s) 157, 461
BslI CCNNNNNNNGG 1 cut(s) 239
BsmAI GTCTC 2 cut(s) 46, 318
BsmBI CGTCTC 2 cut(s) 46, 318
BsmFI GGGAC 2 cut(s) 157, 461
Bsp1286I GDGCHC 1 cut(s) 324
Bsp143I GATC 7 cut(s) 24, 92, 106, 112, 127, 190, 512
BspACI CCGC 9 cut(s) 220, 230, 233, 332, 350, 359, 385, 401, 404
BspFNI CGCG 3 cut(s) 361, 487, 489
BspHI TCATGA 1 cut(s) 124
BspPI GGATC 3 cut(s) 32, 87, 114
BsrFI RCCGGY 1 cut(s) 406
BssAI RCCGGY 1 cut(s) 406
BssECI CCNNGG 2 cut(s) 204, 239
BssHII GCGCGC 2 cut(s) 485, 487
BssMI GATC 7 cut(s) 24, 92, 106, 112, 127, 190, 512
BssNI GRCGYC 1 cut(s) 311
Bst4CI ACNGT 2 cut(s) 102, 147
BstACI GRCGYC 1 cut(s) 311
BstC8I GCNNGC 5 cut(s) 354, 408, 415, 487, 489
BstF5I GGATG 3 cut(s) 277, 286, 487
BstFNI CGCG 3 cut(s) 361, 487, 489
BstH2I RGCGCY 1 cut(s) 359
BstHHI GCGC 5 cut(s) 121, 358, 487, 489, 491
BstKTI GATC 7 cut(s) 27, 95, 109, 115, 130, 193, 515
BstMAI GTCTC 2 cut(s) 46, 318
BstMBI GATC 7 cut(s) 24, 92, 106, 112, 127, 190, 512
BstMCI CGRYCG 2 cut(s) 22, 389
BstMWI GCNNNNNNNGC 3 cut(s) 235, 358, 403
BstSCI CCNGG 3 cut(s) 204, 238, 477
BstUI CGCG 3 cut(s) 361, 487, 489
BstV1I GCAGC 2 cut(s) 222, 397
BstX2I RGATCY 1 cut(s) 24
BstYI RGATCY 1 cut(s) 24
BtsCI GGATG 3 cut(s) 277, 286, 487
Cac8I GCNNGC 5 cut(s) 354, 408, 415, 487, 489
CciI TCATGA 1 cut(s) 124
CfoI GCGC 5 cut(s) 121, 358, 487, 489, 491
Cfr10I RCCGGY 1 cut(s) 406
CviAII CATG 4 cut(s) 79, 116, 125, 277
CviJI RGCY 3 cut(s) 229, 410, 506
CviKI_1 RGCY 3 cut(s) 229, 410, 506
DpnI GATC 7 cut(s) 26, 94, 108, 114, 129, 192, 514
DpnII GATC 7 cut(s) 24, 92, 106, 112, 127, 190, 512
DrdI GACNNNNNNGTC 1 cut(s) 272
DriI GACNNNNNGTC 1 cut(s) 345
DseDI GACNNNNNNGTC 1 cut(s) 272
Eam1105I GACNNNNNGTC 1 cut(s) 345
EciI GGCGGA 2 cut(s) 339, 390
EcoRI GAATTC 1 cut(s) 46
Esp3I CGTCTC 2 cut(s) 46, 318
FaeI CATG 4 cut(s) 82, 119, 128, 280
FaiI YATR 4 cut(s) 80, 117, 126, 278
FaqI GGGAC 2 cut(s) 157, 461
FatI CATG 4 cut(s) 78, 115, 124, 276
FauI CCCGC 1 cut(s) 339
FbaI TGATCA 1 cut(s) 127
FblI GTMKAC 2 cut(s) 18, 257
Fnu4HI GCNGC 6 cut(s) 230, 233, 236, 359, 404, 411
FokI GGATG 3 cut(s) 284, 293, 494
Fsp4HI GCNGC 6 cut(s) 230, 233, 236, 359, 404, 411
FspBI CTAG 1 cut(s) 14
GlaI GCGC 5 cut(s) 120, 357, 486, 488, 490
GluI GCNGC 6 cut(s) 230, 233, 236, 359, 404, 411
GsuI CTGGAG 1 cut(s) 338
HaeII RGCGCY 1 cut(s) 359
HapII CCGG 6 cut(s) 22, 64, 206, 239, 407, 479
HhaI GCGC 5 cut(s) 121, 358, 487, 489, 491
Hin1I GRCGYC 1 cut(s) 311
Hin1II CATG 4 cut(s) 82, 119, 128, 280
Hin6I GCGC 5 cut(s) 119, 356, 485, 487, 489
HinP1I GCGC 5 cut(s) 119, 356, 485, 487, 489
HincII GTYRAC 2 cut(s) 19, 258
HindII GTYRAC 2 cut(s) 19, 258
HinfI GANTC 2 cut(s) 5, 251
HpaII CCGG 6 cut(s) 22, 64, 206, 239, 407, 479
Hpy166II GTNNAC 3 cut(s) 19, 258, 432
Hpy188I TCNGA 3 cut(s) 85, 106, 517
Hpy188III TCNNGA 5 cut(s) 43, 110, 125, 317, 499
Hpy8I GTNNAC 3 cut(s) 19, 258, 432
Hpy99I CGWCG 7 cut(s) 106, 259, 262, 293, 296, 313, 397
HpyAV CCTTC 1 cut(s) 375
HpyCH4III ACNGT 2 cut(s) 102, 147
HpyCH4IV ACGT 2 cut(s) 39, 311
HpyF10VI GCNNNNNNNGC 3 cut(s) 235, 358, 403
HpySE526I ACGT 2 cut(s) 39, 311
Hsp92I GRCGYC 1 cut(s) 311
Hsp92II CATG 4 cut(s) 82, 119, 128, 280
HspAI GCGC 5 cut(s) 119, 356, 485, 487, 489
KroI GCCGGC 1 cut(s) 406
KroNI GCCGGC 1 cut(s) 408
Ksp22I TGATCA 1 cut(s) 127
Kzo9I GATC 7 cut(s) 24, 92, 106, 112, 127, 190, 512
LmnI GCTCC 2 cut(s) 66, 319
Lsp1109I GCAGC 2 cut(s) 222, 397
LweI GCATC 1 cut(s) 472
MaeI CTAG 1 cut(s) 14
MaeII ACGT 2 cut(s) 39, 311
MalI GATC 7 cut(s) 26, 94, 108, 114, 129, 192, 514
MboI GATC 7 cut(s) 24, 92, 106, 112, 127, 190, 512
MboII GAAGA 1 cut(s) 257
MflI RGATCY 1 cut(s) 24
MhlI GDGCHC 1 cut(s) 324
MluCI AATT 1 cut(s) 46
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 2 cut(s) 84, 486
MnlI CCTC 4 cut(s) 44, 132, 277, 408
MroNI GCCGGC 1 cut(s) 406
MseI TTAA 1 cut(s) 198
MslI CAYNNNNRTG 1 cut(s) 114
MspA1I CMGCKG 1 cut(s) 235
MspI CCGG 6 cut(s) 22, 64, 206, 239, 407, 479
MspR9I CCNGG 3 cut(s) 206, 240, 479
MvnI CGCG 3 cut(s) 361, 487, 489
MwoI GCNNNNNNNGC 3 cut(s) 235, 358, 403
NaeI GCCGGC 1 cut(s) 408
NciI CCSGG 3 cut(s) 206, 240, 479
NdeII GATC 7 cut(s) 24, 92, 106, 112, 127, 190, 512
NgoMIV GCCGGC 1 cut(s) 406
NlaIII CATG 4 cut(s) 82, 119, 128, 280
PagI TCATGA 1 cut(s) 124
PauI GCGCGC 2 cut(s) 485, 487
PdiI GCCGGC 1 cut(s) 408
PfeI GAWTC 1 cut(s) 251
PfoI TCCNGGA 1 cut(s) 477
PkrI GCNGC 6 cut(s) 231, 234, 237, 360, 405, 412
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PsuI RGATCY 1 cut(s) 24
PteI GCGCGC 2 cut(s) 485, 487
RseI CAYNNNNRTG 1 cut(s) 114
SalI GTCGAC 2 cut(s) 17, 256
SaqAI TTAA 1 cut(s) 198
SatI GCNGC 6 cut(s) 230, 233, 236, 359, 404, 411
Sau3AI GATC 7 cut(s) 24, 92, 106, 112, 127, 190, 512
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 3 cut(s) 206, 240, 479
SduI GDGCHC 1 cut(s) 324
SetI ASST 4 cut(s) 15, 42, 314, 426
SfaNI GCATC 1 cut(s) 472
SgrDI CGTCGACG 1 cut(s) 256
SmiMI CAYNNNNRTG 1 cut(s) 114
SmlI CTYRAG 1 cut(s) 435
SmoI CTYRAG 1 cut(s) 435
Sse9I AATT 1 cut(s) 46
SsiI CCGC 9 cut(s) 220, 230, 233, 332, 350, 359, 385, 401, 404
SspMI CTAG 1 cut(s) 14
StyD4I CCNGG 3 cut(s) 204, 238, 477
TaaI ACNGT 2 cut(s) 102, 147
TaiI ACGT 2 cut(s) 42, 314
TaqI TCGA 4 cut(s) 18, 44, 91, 257
TasI AATT 1 cut(s) 46
TauI GCSGC 4 cut(s) 232, 235, 361, 406
TfiI GAWTC 1 cut(s) 251
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TseI GCWGC 2 cut(s) 235, 410
TspDTI ATGAA 1 cut(s) 264
TspGWI ACGGA 1 cut(s) 19
XapI RAATTY 1 cut(s) 46
XmiI GTMKAC 2 cut(s) 18, 257
XspI CTAG 1 cut(s) 14
ZraI GACGTC 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.