MD12G1142900.v1.1

Rare lipoprotein A (RlpA)-like double-psi beta-barrel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0015821
Physical Location & Seq
Reverse (-)
22201617 .. 22203793
2177 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1142900.v1.1.491

Sequence Viewer

Length: 777 bp
ATGGCCTTCCTTCGCATGCTCTGCATTGCTTCCCTGCTCTCGCTCTTCATGGCGGCCGACGCCAGAATCCCAGGGGTTTACACTGGTGGCGCATGGGAGGGCGCCCACGCCACCTTCTACGGCGGCAGCGACGCCTCTGGCACCATGGGTGGCGCTTGCGGGTACGGCAACCTCTACAGCCAGGGCTACGGCGTCAACACGGCTGCGCTTAGCACTGCTCTGTTCAACAATGGCCTCAGCTGCGGCGCCTGCTTTGAGATTAAGTGCGGCGACGACCCCAGGTGGTGCCACCCAGGCAACCCCTCCATCTTTGTCACCGCCACCAACTTCTGCCCTCCTAACTTCGCTCAGCCCAGCGACGACGGCGGCTGGTGCAACCCTCCCCGCACCCACTTCGACCTCGCCATGCCCATGTTCCTCAAAATCGCCGAGTACAAGGCCGGCATCGTCCCAGTCTCTTACCGCCGGGTTCCGTGCAGAAAGCAAGGCGGAGTGAGATTCACAATTAACGGTTTCCGTTACTTCAACCTGGTTCTGATCACCAACGTCGCGGGCGCAGGGGATATCGTGAGGGTGAGCGTGAAAGGTGCGAACACGGGGTGGATGCCGATGAGCCGGAACTGGGGGCAAAACTGGCAATCCAACGCAGACCTGGTGGGCCAGACCCTGTCCTTCAGAGTCACGGGCAGTGACAGGCGCACCTCCACCTCCCTCAACGTGGCGCCCGCTCATTGGCAGTTCGGACAAACTTTCACCGGGAAGAATTTCCGCGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

259

Amino Acids

27.85

Weight (kDa)

9.15

Isoelectric Point (pI)

28.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 37 - 161 7.4e-09 EXPB1-like domain 1
DPBB_1 PF03330 67 - 154 2.2e-21 Lytic transglycolase
Expansin_C PF01357 165 - 242 3.4e-30 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015821)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37640 AT2G37640 AT5G02260
fragaria_vesca FvH4_6g13610
malus_domestica MD04G1129800.v1.1 MD12G1142900.v1.1
prunus_persica Prupe.6G256500_v2.0.a1
pyrus_communis pycom04g11810 pycom12g13760
rosa_chinensis RchiOBHm_Chr3g0466461
rosa_laevigata RLG00000024572
rosa_multiflora Rmu_sc0003332.1_g000023
rosa_rugosa Rorug03G0082100
rosa_samantha Rh3DG150700
rosa_wichuraiana Rw3G012170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 5 cut(s) 101, 140, 245, 285, 721
AccBSI CCGCTC 1 cut(s) 728
AccII CGCG 2 cut(s) 551, 771
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 1 cut(s) 763
AcuI CTGAAG 1 cut(s) 658
AcyI GRCGYC 6 cut(s) 60, 102, 132, 192, 246, 722
AdeI CACNNNGTG 1 cut(s) 600
AfaI GTAC 2 cut(s) 164, 434
AfiI CCNNNNNNNGG 3 cut(s) 622, 718, 732
AgsI TTSAA 2 cut(s) 226, 526
AjnI CCWGG 6 cut(s) 70, 180, 278, 292, 528, 651
AluBI AGCT 1 cut(s) 240
AluI AGCT 1 cut(s) 240
Alw26I GTCTC 1 cut(s) 460
AlwNI CAGNNNCTG 1 cut(s) 667
AoxI GGCC 5 cut(s) 3, 54, 232, 438, 658
ApeKI GCWGC 3 cut(s) 126, 203, 240
ApoI RAATTY 1 cut(s) 763
Asp700I GAANNNNTTC 1 cut(s) 764
AspLEI GCGC 8 cut(s) 92, 104, 155, 208, 248, 557, 699, 724
AspS9I GGNCC 1 cut(s) 658
AsuC2I CCSGG 2 cut(s) 467, 757
AsuHPI GGTGA 4 cut(s) 307, 532, 586, 745
BanI GGYRCC 5 cut(s) 101, 140, 245, 285, 721
BbvCI CCTCAGC 1 cut(s) 236
BbvI GCAGC 3 cut(s) 138, 190, 227
BccI CCATC 1 cut(s) 314
BceAI ACGGC 5 cut(s) 136, 181, 205, 216, 379
BcgI CGANNNNNNTGC 2 cut(s) 376, 410
BciT130I CCWGG 6 cut(s) 72, 182, 280, 294, 530, 653
BclI TGATCA 1 cut(s) 537
BcnI CCSGG 2 cut(s) 467, 757
BcoDI GTCTC 1 cut(s) 460
BfmI CTRYAG 1 cut(s) 175
BfoI RGCGCY 4 cut(s) 105, 156, 249, 725
BglI GCCNNNNNGGC 1 cut(s) 294
BisI GCNGC 8 cut(s) 54, 124, 127, 204, 241, 244, 268, 367
BlpI GCTNAGC 2 cut(s) 209, 348
BlsI GCNGC 8 cut(s) 55, 125, 128, 205, 242, 245, 269, 368
Bme1390I CCNGG 8 cut(s) 72, 182, 280, 294, 467, 530, 653, 757
BmgT120I GGNCC 1 cut(s) 658
BmiI GGNNCC 6 cut(s) 103, 142, 247, 287, 471, 723
BmrFI CCNGG 8 cut(s) 72, 182, 280, 294, 467, 530, 653, 757
BmrI ACTGGG 2 cut(s) 446, 631
BmsI GCATC 2 cut(s) 453, 594
BmuI ACTGGG 2 cut(s) 446, 631
Bpu10I CCTNAGC 1 cut(s) 236
Bpu1102I GCTNAGC 2 cut(s) 209, 348
BpuMI CCSGG 2 cut(s) 467, 757
BsaHI GRCGYC 6 cut(s) 60, 102, 132, 192, 246, 722
BsaJI CCNNGG 6 cut(s) 70, 71, 144, 181, 278, 292
Bsc4I CCNNNNNNNGG 3 cut(s) 622, 718, 732
Bse118I RCCGGY 1 cut(s) 440
Bse1I ACTGG 4 cut(s) 88, 452, 626, 638
Bse3DI GCAATG 1 cut(s) 24
BseBI CCWGG 6 cut(s) 72, 182, 280, 294, 530, 653
BseDI CCNNGG 6 cut(s) 70, 71, 144, 181, 278, 292
BseGI GGATG 1 cut(s) 609
BseLI CCNNNNNNNGG 3 cut(s) 622, 718, 732
BseMI GCAATG 1 cut(s) 24
BseMII CTCAG 2 cut(s) 250, 362
BseNI ACTGG 4 cut(s) 88, 452, 626, 638
BseX3I CGGCCG 1 cut(s) 54
BseXI GCAGC 3 cut(s) 138, 190, 227
BseYI CCCAGC 1 cut(s) 353
BsgI GTGCAG 1 cut(s) 496
Bsh1236I CGCG 2 cut(s) 551, 771
Bsh1285I CGRYCG 1 cut(s) 57
BshFI GGCC 5 cut(s) 5, 56, 234, 440, 660
BshNI GGYRCC 5 cut(s) 101, 140, 245, 285, 721
BsiEI CGRYCG 1 cut(s) 57
BsiSI CCGG 4 cut(s) 441, 466, 616, 756
BslFI GGGAC 1 cut(s) 434
BslI CCNNNNNNNGG 3 cut(s) 622, 718, 732
BsmAI GTCTC 1 cut(s) 460
BsmFI GGGAC 1 cut(s) 434
BsnI GGCC 5 cut(s) 5, 56, 234, 440, 660
Bsp143I GATC 1 cut(s) 537
Bsp1720I GCTNAGC 2 cut(s) 209, 348
Bsp19I CCATGG 1 cut(s) 144
BspANI GGCC 5 cut(s) 5, 56, 234, 440, 660
BspCNI CTCAG 2 cut(s) 249, 361
BspFNI CGCG 2 cut(s) 551, 771
BspLI GGNNCC 6 cut(s) 103, 142, 247, 287, 471, 723
BspQI GCTCTTC 1 cut(s) 50
BspT107I GGYRCC 5 cut(s) 101, 140, 245, 285, 721
BsrBI CCGCTC 1 cut(s) 728
BsrDI GCAATG 1 cut(s) 24
BsrFI RCCGGY 1 cut(s) 440
BsrI ACTGG 4 cut(s) 88, 452, 626, 638
BssAI RCCGGY 1 cut(s) 440
BssECI CCNNGG 6 cut(s) 70, 71, 144, 181, 278, 292
BssMI GATC 1 cut(s) 537
BssNI GRCGYC 6 cut(s) 60, 102, 132, 192, 246, 722
BssT1I CCWWGG 1 cut(s) 144
Bst2UI CCWGG 6 cut(s) 72, 182, 280, 294, 530, 653
Bst4CI ACNGT 1 cut(s) 512
Bst6I CTCTTC 1 cut(s) 50
BstACI GRCGYC 6 cut(s) 60, 102, 132, 192, 246, 722
BstAPI GCANNNNNTGC 1 cut(s) 21
BstC8I GCNNGC 6 cut(s) 17, 157, 250, 442, 553, 726
BstDEI CTNAG 3 cut(s) 209, 236, 348
BstDSI CCRYGG 1 cut(s) 144
BstF5I GGATG 1 cut(s) 609
BstFNI CGCG 2 cut(s) 551, 771
BstH2I RGCGCY 4 cut(s) 105, 156, 249, 725
BstHHI GCGC 8 cut(s) 92, 104, 155, 208, 248, 557, 699, 724
BstKTI GATC 1 cut(s) 540
BstMAI GTCTC 1 cut(s) 460
BstMBI GATC 1 cut(s) 537
BstMCI CGRYCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 9 cut(s) 21, 59, 165, 240, 249, 294, 363, 372, 634
BstNI CCWGG 6 cut(s) 72, 182, 280, 294, 530, 653
BstNSI RCATGY 1 cut(s) 19
BstSCI CCNGG 8 cut(s) 70, 180, 278, 292, 465, 528, 651, 755
BstSFI CTRYAG 1 cut(s) 175
BstUI CGCG 2 cut(s) 551, 771
BstV1I GCAGC 3 cut(s) 138, 190, 227
BstZI CGGCCG 1 cut(s) 54
BsuRI GGCC 5 cut(s) 5, 56, 234, 440, 660
BtgI CCRYGG 1 cut(s) 144
BtsCI GGATG 1 cut(s) 609
BtsI GCAGTG 2 cut(s) 213, 694
BtsIMutI CAGTG 3 cut(s) 81, 213, 694
Cac8I GCNNGC 6 cut(s) 17, 157, 250, 442, 553, 726
CaiI CAGNNNCTG 1 cut(s) 667
CfoI GCGC 8 cut(s) 92, 104, 155, 208, 248, 557, 699, 724
Cfr10I RCCGGY 1 cut(s) 440
Cfr13I GGNCC 1 cut(s) 658
CseI GACGC 4 cut(s) 68, 140, 181, 760
CsiI ACCWGGT 2 cut(s) 528, 651
Csp6I GTAC 2 cut(s) 163, 433
CviAII CATG 6 cut(s) 16, 49, 93, 145, 406, 412
CviQI GTAC 2 cut(s) 163, 433
DdeI CTNAG 3 cut(s) 209, 236, 348
DinI GGCGCC 3 cut(s) 103, 247, 723
DpnI GATC 1 cut(s) 539
DpnII GATC 1 cut(s) 537
DraIII CACNNNGTG 1 cut(s) 600
EaeI YGGCCR 1 cut(s) 54
EagI CGGCCG 1 cut(s) 54
Eam1104I CTCTTC 1 cut(s) 50
EarI CTCTTC 1 cut(s) 50
EciI GGCGGA 1 cut(s) 504
EclXI CGGCCG 1 cut(s) 54
Eco130I CCWWGG 1 cut(s) 144
Eco32I GATATC 1 cut(s) 565
Eco52I CGGCCG 1 cut(s) 54
Eco57I CTGAAG 1 cut(s) 658
EcoRII CCWGG 6 cut(s) 70, 180, 278, 292, 528, 651
EcoRV GATATC 1 cut(s) 565
EcoT14I CCWWGG 1 cut(s) 144
EgeI GGCGCC 3 cut(s) 103, 247, 723
EheI GGCGCC 3 cut(s) 103, 247, 723
ErhI CCWWGG 1 cut(s) 144
FaeI CATG 6 cut(s) 19, 52, 96, 148, 409, 415
FaiI YATR 6 cut(s) 17, 50, 94, 146, 407, 413
FaqI GGGAC 1 cut(s) 434
FatI CATG 6 cut(s) 15, 48, 92, 144, 405, 411
FauI CCCGC 4 cut(s) 152, 392, 544, 733
FbaI TGATCA 1 cut(s) 537
Fnu4HI GCNGC 8 cut(s) 54, 124, 127, 204, 241, 244, 268, 367
FokI GGATG 1 cut(s) 616
Fsp4HI GCNGC 8 cut(s) 54, 124, 127, 204, 241, 244, 268, 367
GlaI GCGC 8 cut(s) 91, 103, 154, 207, 247, 556, 698, 723
GluI GCNGC 8 cut(s) 54, 124, 127, 204, 241, 244, 268, 367
GsaI CCCAGC 1 cut(s) 357
HaeII RGCGCY 4 cut(s) 105, 156, 249, 725
HaeIII GGCC 5 cut(s) 5, 56, 234, 440, 660
HapII CCGG 4 cut(s) 441, 466, 616, 756
HgaI GACGC 4 cut(s) 68, 140, 181, 760
HhaI GCGC 8 cut(s) 92, 104, 155, 208, 248, 557, 699, 724
Hin1I GRCGYC 6 cut(s) 60, 102, 132, 192, 246, 722
Hin1II CATG 6 cut(s) 19, 52, 96, 148, 409, 415
Hin6I GCGC 8 cut(s) 90, 102, 153, 206, 246, 555, 697, 722
HinP1I GCGC 8 cut(s) 90, 102, 153, 206, 246, 555, 697, 722
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HinfI GANTC 3 cut(s) 66, 498, 678
HpaII CCGG 4 cut(s) 441, 466, 616, 756
HphI GGTGA 4 cut(s) 307, 532, 586, 745
Hpy166II GTNNAC 2 cut(s) 79, 196
Hpy188I TCNGA 3 cut(s) 537, 677, 743
Hpy188III TCNNGA 1 cut(s) 568
Hpy8I GTNNAC 2 cut(s) 79, 196
Hpy99I CGWCG 6 cut(s) 62, 134, 275, 362, 365, 551
HpyAV CCTTC 4 cut(s) 16, 20, 124, 682
HpyCH4III ACNGT 1 cut(s) 512
HpyCH4IV ACGT 2 cut(s) 546, 717
HpyCH4V TGCA 3 cut(s) 24, 375, 477
HpyF10VI GCNNNNNNNGC 9 cut(s) 21, 59, 165, 240, 249, 294, 363, 372, 634
HpyF3I CTNAG 3 cut(s) 209, 236, 348
HpySE526I ACGT 2 cut(s) 546, 717
Hsp92I GRCGYC 6 cut(s) 60, 102, 132, 192, 246, 722
Hsp92II CATG 6 cut(s) 19, 52, 96, 148, 409, 415
HspAI GCGC 8 cut(s) 90, 102, 153, 206, 246, 555, 697, 722
KasI GGCGCC 3 cut(s) 101, 245, 721
KroI GCCGGC 1 cut(s) 440
KroNI GCCGGC 1 cut(s) 442
Ksp22I TGATCA 1 cut(s) 537
Kzo9I GATC 1 cut(s) 537
LguI GCTCTTC 1 cut(s) 50
Lsp1109I GCAGC 3 cut(s) 138, 190, 227
LweI GCATC 2 cut(s) 453, 594
MabI ACCWGGT 2 cut(s) 528, 651
MaeII ACGT 2 cut(s) 546, 717
MaeIII GTNAC 4 cut(s) 313, 518, 679, 689
MalI GATC 1 cut(s) 539
MbiI CCGCTC 1 cut(s) 728
MboI GATC 1 cut(s) 537
MboII GAAGA 2 cut(s) 37, 772
MluCI AATT 2 cut(s) 504, 763
Mly113I GGCGCC 3 cut(s) 102, 246, 722
MlyI GAGTC 1 cut(s) 687
MmeI TCCRAC 1 cut(s) 666
MroNI GCCGGC 1 cut(s) 440
MroXI GAANNNNTTC 1 cut(s) 764
MseI TTAA 2 cut(s) 261, 507
MslI CAYNNNNRTG 1 cut(s) 410
MspA1I CMGCKG 1 cut(s) 240
MspI CCGG 4 cut(s) 441, 466, 616, 756
MspR9I CCNGG 8 cut(s) 72, 182, 280, 294, 467, 530, 653, 757
MvaI CCWGG 6 cut(s) 72, 182, 280, 294, 530, 653
MvnI CGCG 2 cut(s) 551, 771
MwoI GCNNNNNNNGC 9 cut(s) 21, 59, 165, 240, 249, 294, 363, 372, 634
NaeI GCCGGC 1 cut(s) 442
NarI GGCGCC 3 cut(s) 102, 246, 722
NciI CCSGG 2 cut(s) 467, 757
NcoI CCATGG 1 cut(s) 144
NdeII GATC 1 cut(s) 537
NgoMIV GCCGGC 1 cut(s) 440
NlaIII CATG 6 cut(s) 19, 52, 96, 148, 409, 415
NlaIV GGNNCC 6 cut(s) 103, 142, 247, 287, 471, 723
NmeAIII GCCGAG 1 cut(s) 454
NmuCI GTSAC 3 cut(s) 313, 679, 689
NspI RCATGY 1 cut(s) 19
PaeI GCATGC 1 cut(s) 19
PasI CCCWGGG 1 cut(s) 71
PciSI GCTCTTC 1 cut(s) 50
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PdiI GCCGGC 1 cut(s) 442
PdmI GAANNNNTTC 1 cut(s) 764
PfeI GAWTC 2 cut(s) 66, 498
PflFI GACNNNGTC 1 cut(s) 667
PkrI GCNGC 8 cut(s) 55, 125, 128, 205, 242, 245, 269, 368
PleI GAGTC 1 cut(s) 686
PluTI GGCGCC 3 cut(s) 105, 249, 725
PpsI GAGTC 1 cut(s) 686
Psp6I CCWGG 6 cut(s) 70, 180, 278, 292, 528, 651
PspFI CCCAGC 1 cut(s) 353
PspGI CCWGG 6 cut(s) 70, 180, 278, 292, 528, 651
PspN4I GGNNCC 6 cut(s) 103, 142, 247, 287, 471, 723
PspPI GGNCC 1 cut(s) 658
PstNI CAGNNNCTG 1 cut(s) 667
PsyI GACNNNGTC 1 cut(s) 667
PvuII CAGCTG 1 cut(s) 240
RsaI GTAC 2 cut(s) 164, 434
RsaNI GTAC 2 cut(s) 163, 433
RseI CAYNNNNRTG 1 cut(s) 410
SapI GCTCTTC 1 cut(s) 50
SaqAI TTAA 2 cut(s) 261, 507
SatI GCNGC 8 cut(s) 54, 124, 127, 204, 241, 244, 268, 367
Sau3AI GATC 1 cut(s) 537
Sau96I GGNCC 1 cut(s) 658
SchI GAGTC 1 cut(s) 687
ScrFI CCNGG 8 cut(s) 72, 182, 280, 294, 467, 530, 653, 757
SexAI ACCWGGT 2 cut(s) 528, 651
SfaNI GCATC 2 cut(s) 453, 594
SfcI CTRYAG 1 cut(s) 175
SfoI GGCGCC 3 cut(s) 103, 247, 723
SmiMI CAYNNNNRTG 1 cut(s) 410
SphI GCATGC 1 cut(s) 19
Sse9I AATT 2 cut(s) 504, 763
SspDI GGCGCC 3 cut(s) 101, 245, 721
StyD4I CCNGG 8 cut(s) 70, 180, 278, 292, 465, 528, 651, 755
StyI CCWWGG 1 cut(s) 144
TaaI ACNGT 1 cut(s) 512
TaiI ACGT 2 cut(s) 549, 720
TaqI TCGA 1 cut(s) 396
TasI AATT 2 cut(s) 504, 763
TatI WGTACW 1 cut(s) 432
TauI GCSGC 5 cut(s) 56, 126, 246, 270, 369
TfiI GAWTC 2 cut(s) 66, 498
Tru1I TTAA 2 cut(s) 261, 507
Tru9I TTAA 2 cut(s) 261, 507
TscAI CASTG 3 cut(s) 88, 220, 694
TseFI GTSAC 3 cut(s) 313, 679, 689
TseI GCWGC 3 cut(s) 126, 203, 240
Tsp45I GTSAC 3 cut(s) 313, 679, 689
TspDTI ATGAA 1 cut(s) 37
TspGWI ACGGA 2 cut(s) 462, 506
TspRI CASTG 3 cut(s) 88, 220, 694
Tth111I GACNNNGTC 1 cut(s) 667
XapI RAATTY 1 cut(s) 763
XceI RCATGY 1 cut(s) 19
XcmI CCANNNNNNNNNTGG 1 cut(s) 649
XmnI GAANNNNTTC 1 cut(s) 764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.