MD12G1153100.v1.1

Receptor-like protein 12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0018507
Physical Location & Seq
Reverse (-)
23307065 .. 23309332
2268 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1153100.v1.1.491

Sequence Viewer

Length: 420 bp
ATGCCAAGTTCAATAGATTATAATAATGAAATCCAGGAACGGGATTTCACAGAAGACCTTCATCAATCGGTTTATTTTGACTTGTCATTAAACAAGTTCAATGGTTCAGTTCCATCCTTCAGTATGGCAAAAAATCTGACCCTGATCAATCTTTCTAACAATCAGCTAACGGGTCAGATTAATTCCACTCACTGGGAAAACCTAACTAATCTGATGAATCTCAACTTGAGATCCAATCAATTAGATGGAACTATTCTGCCATCTCTGTTTTCTCTTCCCTTGCTGCAAAAGCTACAACTTTCCAGCAATCACTTCTCTGGTCATTTTCCAGAATTTGGTAATATTTGTTCCCCATACACCTTTGATTTGAGTAACAACAGTTTGGAAGGGCCTATACCCGTGTCAGTCTTCAATCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

140

Amino Acids

15.67

Weight (kDa)

4.72

Isoelectric Point (pI)

49.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 21
AccB7I CCANNNNNTGG 2 cut(s) 192, 335
AclWI GGATC 1 cut(s) 225
AcsI RAATTY 1 cut(s) 332
AcuI CTGAAG 1 cut(s) 103
AfiI CCNNNNNNNGG 3 cut(s) 40, 192, 335
AgsI TTSAA 3 cut(s) 12, 100, 412
AjnI CCWGG 1 cut(s) 33
AluBI AGCT 2 cut(s) 166, 292
AluI AGCT 2 cut(s) 166, 292
AlwI GGATC 1 cut(s) 225
AoxI GGCC 1 cut(s) 389
ApeKI GCWGC 1 cut(s) 283
ApoI RAATTY 1 cut(s) 332
AseI ATTAAT 1 cut(s) 180
Asp700I GAANNNNTTC 1 cut(s) 57
AspS9I GGNCC 1 cut(s) 389
BarI GAAGNNNNNNTAC 2 cut(s) 378, 410
BbsI GAAGAC 2 cut(s) 60, 400
BbvI GCAGC 1 cut(s) 270
BccI CCATC 3 cut(s) 121, 239, 268
BciT130I CCWGG 1 cut(s) 35
BclI TGATCA 1 cut(s) 144
BisI GCNGC 1 cut(s) 284
BlsI GCNGC 1 cut(s) 285
Bme1390I CCNGG 1 cut(s) 35
BmgT120I GGNCC 1 cut(s) 389
BmrFI CCNGG 1 cut(s) 35
BmrI ACTGGG 1 cut(s) 202
BmuI ACTGGG 1 cut(s) 202
BpiI GAAGAC 2 cut(s) 60, 400
BpuEI CTTGAG 1 cut(s) 247
Bsc4I CCNNNNNNNGG 3 cut(s) 40, 192, 335
Bse1I ACTGG 1 cut(s) 197
BseBI CCWGG 1 cut(s) 35
BseGI GGATG 1 cut(s) 113
BseLI CCNNNNNNNGG 3 cut(s) 40, 192, 335
BseNI ACTGG 1 cut(s) 197
BseXI GCAGC 1 cut(s) 270
BshFI GGCC 1 cut(s) 391
BslI CCNNNNNNNGG 3 cut(s) 40, 192, 335
BsnI GGCC 1 cut(s) 391
Bsp143I GATC 2 cut(s) 144, 230
BspANI GGCC 1 cut(s) 391
BspPI GGATC 1 cut(s) 225
BsrI ACTGG 1 cut(s) 197
BssMI GATC 2 cut(s) 144, 230
Bst2UI CCWGG 1 cut(s) 35
Bst4CI ACNGT 1 cut(s) 380
Bst6I CTCTTC 1 cut(s) 279
BstF5I GGATG 1 cut(s) 113
BstKTI GATC 2 cut(s) 147, 233
BstMBI GATC 2 cut(s) 144, 230
BstMWI GCNNNNNNNGC 1 cut(s) 289
BstNI CCWGG 1 cut(s) 35
BstSCI CCNGG 1 cut(s) 33
BstV1I GCAGC 1 cut(s) 270
BstV2I GAAGAC 2 cut(s) 60, 400
BstX2I RGATCY 1 cut(s) 230
BstYI RGATCY 1 cut(s) 230
BsuRI GGCC 1 cut(s) 391
BtsCI GGATG 1 cut(s) 113
BtsIMutI CAGTG 1 cut(s) 190
Cfr13I GGNCC 1 cut(s) 389
CviJI RGCY 3 cut(s) 166, 292, 391
CviKI_1 RGCY 3 cut(s) 166, 292, 391
DpnI GATC 2 cut(s) 146, 232
DpnII GATC 2 cut(s) 144, 230
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
Eco57I CTGAAG 1 cut(s) 103
EcoO109I RGGNCCY 1 cut(s) 389
EcoRII CCWGG 1 cut(s) 33
FaiI YATR 5 cut(s) 21, 125, 355, 395, 418
FbaI TGATCA 1 cut(s) 144
Fnu4HI GCNGC 1 cut(s) 284
FokI GGATG 1 cut(s) 100
Fsp4HI GCNGC 1 cut(s) 284
GluI GCNGC 1 cut(s) 284
HaeIII GGCC 1 cut(s) 391
HinfI GANTC 1 cut(s) 217
Hpy188I TCNGA 3 cut(s) 138, 177, 213
Hpy188III TCNNGA 1 cut(s) 329
HpyAV CCTTC 3 cut(s) 68, 127, 380
HpyCH4III ACNGT 1 cut(s) 380
HpyCH4V TGCA 1 cut(s) 286
HpyF10VI GCNNNNNNNGC 1 cut(s) 289
Ksp22I TGATCA 1 cut(s) 144
Kzo9I GATC 2 cut(s) 144, 230
LpnPI CCDG 7 cut(s) 20, 47, 155, 178, 303, 316, 342
Lsp1109I GCAGC 1 cut(s) 270
MaeIII GTNAC 1 cut(s) 371
MalI GATC 2 cut(s) 146, 232
MboI GATC 2 cut(s) 144, 230
MboII GAAGA 3 cut(s) 65, 266, 400
MflI RGATCY 1 cut(s) 230
MluCI AATT 3 cut(s) 181, 239, 332
MroXI GAANNNNTTC 1 cut(s) 57
MseI TTAA 2 cut(s) 89, 180
MspR9I CCNGG 1 cut(s) 35
MvaI CCWGG 1 cut(s) 35
MwoI GCNNNNNNNGC 1 cut(s) 289
NdeII GATC 2 cut(s) 144, 230
PdmI GAANNNNTTC 1 cut(s) 57
PfeI GAWTC 1 cut(s) 217
PflMI CCANNNNNTGG 2 cut(s) 192, 335
PfoI TCCNGGA 1 cut(s) 33
PkrI GCNGC 1 cut(s) 285
PshBI ATTAAT 1 cut(s) 180
PsiI TTATAA 1 cut(s) 21
Psp6I CCWGG 1 cut(s) 33
PspGI CCWGG 1 cut(s) 33
PspPI GGNCC 1 cut(s) 389
PsrI GAACNNNNNNTAC 2 cut(s) 331, 363
PsuI RGATCY 1 cut(s) 230
SaqAI TTAA 2 cut(s) 89, 180
SatI GCNGC 1 cut(s) 284
Sau3AI GATC 2 cut(s) 144, 230
Sau96I GGNCC 1 cut(s) 389
ScrFI CCNGG 1 cut(s) 35
SetI ASST 5 cut(s) 60, 168, 204, 294, 362
SmlI CTYRAG 1 cut(s) 226
SmoI CTYRAG 1 cut(s) 226
Sse9I AATT 3 cut(s) 181, 239, 332
SspI AATATT 1 cut(s) 343
StyD4I CCNGG 1 cut(s) 33
TaaI ACNGT 1 cut(s) 380
TasI AATT 3 cut(s) 181, 239, 332
TfiI GAWTC 1 cut(s) 217
Tru1I TTAA 2 cut(s) 89, 180
Tru9I TTAA 2 cut(s) 89, 180
TscAI CASTG 1 cut(s) 197
TseI GCWGC 1 cut(s) 283
TspDTI ATGAA 3 cut(s) 42, 50, 230
TspRI CASTG 1 cut(s) 197
Van91I CCANNNNNTGG 2 cut(s) 192, 335
VspI ATTAAT 1 cut(s) 180
XapI RAATTY 1 cut(s) 332
XmnI GAANNNNTTC 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.