Rorug01G0234600

Receptor-like protein 12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0018507
Physical Location & Seq
Forward (+)
33768252 .. 33771441
3190 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0234600.1

Sequence Viewer

Length: 1389 bp
ATGAGCCAGTGTGTTCCCAGCTGGGATCTTGATAACAGTAGTCCATCGCCACCGCCGAGGCTCTCTCTTCGTTCTCACTCCAATTCCACCGCTCCTGATGTTCCCATGTTGGACTATGAGGTAGCAGAGCTGACATGGGAAAATGGCCAGGTGGCCATGCACGGCTTGGGTCTACCGCGCTTGCCTTCCAAACCAGCATCCTGGGACAAGCCACGCGCTACTGGTACTCTGGAGTCCATAGTCAACCAAGCCACCTGTACTTTGCCTCGGTTTTCCAAGCCTCCCTTTGATAACACCATCACTTCTGCCACTGGTGGTGGTGCTAAAGAAGTAGTGACCTGGTTTGATCATCTCCGTCGTGTTGCCACCTCCGGTGCTCCCTCCAACACCACCATGACCATGGACGCGTTGGTCCCTTGCAGGAACAACCAACCTGATGAACCAAACAGTAGCCTTATGATAGACTCTGTAGATACCGTGCCAGGAGTCCTCGGCAGTTCTGGCACGGGCATGATCAATGGGTGCTCCACTCGAGTGGGGTCGTGCAGCGCTACCGCGACCCACAACGAGGACACCCGCCGACTGGGAAAACACAAGGCGGGCGTTGCACGTGTACCTGCTACACCCGAGTGCAGATCCCCCAGCGTGAGCGGTAGTGCCACGTTTGGGATGGATAGCCAGCAAGCCACACTTGATACTAAGGGAAGCGACCGTGACTCAGTTTGTCACAGTAGACCACAGAGAGAGGAAAGCGACGAGGAGGAGAGAAAGAAGCGAAGTACTGGAAAATCCTCAGTTTCTACAAAAAGGAGTAGGGCTGCTGCTATACATAACCAATCTGAACGTAAAAGGAGAGATAAGATCAACCAGAGGATGAAGACGCTGCAGAAGCTGGTCCCAAATTCCAGTAAGACTGATAAAGCTTCAATGCTGGACGAAGTGATTGAATACTTGAAACAATTGCAAGCTCAAGTCCATGTGATAAGCAGAATGAACATACCAGCCATGATGTTGCCGATGGCTATGCAGCAACAACTTCAAATGTCTATGATGGCCCCAAGAAATATGGGCATGGGGATTGGGATGGGAATGGGAATGGGAATGGGAATGGGAATGGGAATGGACATGAACAACATCGGCCGCCCCAACATAGGGGGAGTCCCTCCGGTTCTTCACCCAACTGCATTTATGCCCATGGCTTCATGGGATGGCTCTGCCTGCGGCGCTGAGAGGTTACAAGCTGCTTCAACGACTGTGATGCCTGATCCCTTGCCAGCATTTCTTGCATGCCAATCGCAGCCGATGTCGACCATGGATGCTTACAGCATGATGGCGGCCATGTATCAACAATTCCATCAGCAACCAGCAGCATCTAGTTCTAAGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

462

Amino Acids

49.8

Weight (kDa)

8.82

Isoelectric Point (pI)

65.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 277 - 322 4.4e-14 Helix-loop-helix DNA-binding domain
bHLH_SAC51 PF23173 280 - 326 2.1e-08 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 410
Acc36I ACCTGC 1 cut(s) 625
AccBSI CCGCTC 2 cut(s) 92, 651
AccI GTMKAC 3 cut(s) 172, 733, 1307
AccII CGCG 4 cut(s) 178, 216, 407, 557
AclWI GGATC 3 cut(s) 33, 630, 1259
AcoI YGGCCR 4 cut(s) 145, 153, 1138, 1335
AcsI RAATTY 1 cut(s) 901
AcvI CACGTG 1 cut(s) 611
AfaI GTAC 4 cut(s) 226, 259, 615, 781
AfeI AGCGCT 1 cut(s) 550
AfiI CCNNNNNNNGG 5 cut(s) 568, 583, 666, 1151, 1152
AflIII ACRYGT 2 cut(s) 405, 610
AgsI TTSAA 5 cut(s) 927, 947, 955, 1040, 1248
AjnI CCWGG 4 cut(s) 147, 200, 338, 481
AleI CACNNNNGTG 2 cut(s) 533, 628
AluBI AGCT 7 cut(s) 21, 130, 892, 923, 968, 1241, 1386
AluI AGCT 7 cut(s) 21, 130, 892, 923, 968, 1241, 1386
Alw21I GWGCWC 2 cut(s) 379, 527
AlwI GGATC 3 cut(s) 33, 630, 1259
AlwNI CAGNNNCTG 1 cut(s) 892
Ama87I CYCGRG 2 cut(s) 531, 626
Aor51HI AGCGCT 1 cut(s) 550
AoxI GGCC 5 cut(s) 145, 153, 1053, 1138, 1335
ApeKI GCWGC 8 cut(s) 546, 818, 821, 883, 1027, 1241, 1297, 1367
ApoI RAATTY 1 cut(s) 901
AspLEI GCGC 4 cut(s) 180, 218, 551, 1226
AspS9I GGNCC 3 cut(s) 412, 895, 1054
AsuHPI GGTGA 1 cut(s) 1166
AvaI CYCGRG 2 cut(s) 531, 626
AvaII GGWCC 2 cut(s) 412, 895
BalI TGGCCA 2 cut(s) 147, 155
BbrPI CACGTG 1 cut(s) 611
BbsI GAAGAC 1 cut(s) 884
Bbv12I GWGCWC 2 cut(s) 379, 527
BbvI GCAGC 8 cut(s) 558, 805, 808, 870, 1039, 1228, 1309, 1379
BccI CCATC 9 cut(s) 52, 305, 664, 1012, 1045, 1078, 1202, 1324, 1362
BceAI ACGGC 1 cut(s) 178
BcgI CGANNNNNNTGC 6 cut(s) 1006, 1040, 1240, 1274, 1275, 1309
BciT130I CCWGG 4 cut(s) 149, 202, 340, 483
BclI TGATCA 2 cut(s) 346, 513
BfaI CTAG 1 cut(s) 1374
BfmI CTRYAG 2 cut(s) 468, 884
BfoI RGCGCY 2 cut(s) 552, 1227
BfuAI ACCTGC 1 cut(s) 625
BmcAI AGTACT 1 cut(s) 781
Bme1390I CCNGG 4 cut(s) 149, 202, 340, 483
Bme18I GGWCC 2 cut(s) 412, 895
BmeT110I CYCGRG 2 cut(s) 531, 626
BmgT120I GGNCC 3 cut(s) 412, 895, 1054
BmiI GGNNCC 3 cut(s) 414, 897, 1056
BmrFI CCNGG 4 cut(s) 149, 202, 340, 483
BmrI ACTGGG 1 cut(s) 593
BmsI GCATC 4 cut(s) 206, 1248, 1306, 1379
BmuI ACTGGG 1 cut(s) 593
BpiI GAAGAC 1 cut(s) 884
BplI GAGNNNNNCTC 2 cut(s) 49, 81
BpmI CTGGAG 1 cut(s) 251
BpuEI CTTGAG 1 cut(s) 954
BsaAI YACGTR 1 cut(s) 611
BsaJI CCNNGG 7 cut(s) 56, 201, 266, 399, 490, 1194, 1311
BsaWI WCCGGW 2 cut(s) 371, 1165
Bsc4I CCNNNNNNNGG 5 cut(s) 568, 583, 666, 1151, 1152
Bse1I ACTGG 6 cut(s) 7, 226, 316, 588, 787, 906
BseBI CCWGG 4 cut(s) 149, 202, 340, 483
BseDI CCNNGG 7 cut(s) 56, 201, 266, 399, 490, 1194, 1311
BseGI GGATG 6 cut(s) 197, 675, 879, 1089, 1213, 1321
BseLI CCNNNNNNNGG 5 cut(s) 568, 583, 666, 1151, 1152
BseMII CTCAG 3 cut(s) 732, 807, 1218
BseNI ACTGG 6 cut(s) 7, 226, 316, 588, 787, 906
BseRI GAGGAG 2 cut(s) 773, 776
BseX3I CGGCCG 1 cut(s) 1138
BseXI GCAGC 8 cut(s) 558, 805, 808, 870, 1039, 1228, 1309, 1379
BseYI CCCAGC 3 cut(s) 17, 21, 641
BsgI GTGCAG 2 cut(s) 565, 652
Bsh1236I CGCG 4 cut(s) 178, 216, 407, 557
Bsh1285I CGRYCG 2 cut(s) 712, 1141
BshFI GGCC 5 cut(s) 147, 155, 1055, 1140, 1337
BsiEI CGRYCG 2 cut(s) 712, 1141
BsiHKAI GWGCWC 2 cut(s) 379, 527
BsiHKCI CYCGRG 2 cut(s) 531, 626
BsiSI CCGG 2 cut(s) 372, 1166
BslFI GGGAC 4 cut(s) 218, 398, 881, 1145
BslI CCNNNNNNNGG 5 cut(s) 568, 583, 666, 1151, 1152
BsmFI GGGAC 4 cut(s) 218, 398, 881, 1145
BsnI GGCC 5 cut(s) 147, 155, 1055, 1140, 1337
BsoBI CYCGRG 2 cut(s) 531, 626
Bsp1286I GDGCHC 2 cut(s) 379, 527
Bsp143I GATC 6 cut(s) 25, 346, 513, 635, 861, 1264
Bsp19I CCATGG 3 cut(s) 399, 1194, 1311
BspANI GGCC 5 cut(s) 147, 155, 1055, 1140, 1337
BspCNI CTCAG 3 cut(s) 731, 806, 1219
BspFNI CGCG 4 cut(s) 178, 216, 407, 557
BspLI GGNNCC 3 cut(s) 414, 897, 1056
BspMAI CTGCAG 1 cut(s) 888
BspMI ACCTGC 1 cut(s) 625
BspPI GGATC 3 cut(s) 33, 630, 1259
BsrBI CCGCTC 2 cut(s) 92, 651
BsrI ACTGG 6 cut(s) 7, 226, 316, 588, 787, 906
BssECI CCNNGG 7 cut(s) 56, 201, 266, 399, 490, 1194, 1311
BssMI GATC 6 cut(s) 25, 346, 513, 635, 861, 1264
BssT1I CCWWGG 3 cut(s) 399, 1194, 1311
Bst2UI CCWGG 4 cut(s) 149, 202, 340, 483
Bst4CI ACNGT 6 cut(s) 38, 449, 478, 713, 731, 1255
Bst6I CTCTTC 1 cut(s) 72
BstAPI GCANNNNNTGC 1 cut(s) 1283
BstBAI YACGTR 1 cut(s) 611
BstC8I GCNNGC 8 cut(s) 182, 601, 680, 684, 966, 1219, 1275, 1288
BstDEI CTNAG 5 cut(s) 699, 718, 793, 1227, 1380
BstDSI CCRYGG 3 cut(s) 399, 1194, 1311
BstF5I GGATG 6 cut(s) 197, 675, 879, 1089, 1213, 1321
BstFNI CGCG 4 cut(s) 178, 216, 407, 557
BstH2I RGCGCY 2 cut(s) 552, 1227
BstHHI GCGC 4 cut(s) 180, 218, 551, 1226
BstKTI GATC 6 cut(s) 28, 349, 516, 638, 864, 1267
BstMBI GATC 6 cut(s) 25, 346, 513, 635, 861, 1264
BstMCI CGRYCG 2 cut(s) 712, 1141
BstMWI GCNNNNNNNGC 6 cut(s) 501, 605, 889, 1218, 1223, 1283
BstNI CCWGG 4 cut(s) 149, 202, 340, 483
BstNSI RCATGY 1 cut(s) 1290
BstSCI CCNGG 4 cut(s) 147, 200, 338, 481
BstSFI CTRYAG 2 cut(s) 468, 884
BstUI CGCG 4 cut(s) 178, 216, 407, 557
BstV1I GCAGC 8 cut(s) 558, 805, 808, 870, 1039, 1228, 1309, 1379
BstV2I GAAGAC 1 cut(s) 884
BstX2I RGATCY 2 cut(s) 25, 635
BstXI CCANNNNNNTGG 3 cut(s) 201, 400, 535
BstYI RGATCY 2 cut(s) 25, 635
BstZI CGGCCG 1 cut(s) 1138
BsuRI GGCC 5 cut(s) 147, 155, 1055, 1140, 1337
BtgI CCRYGG 3 cut(s) 399, 1194, 1311
BtgZI GCGATG 1 cut(s) 30
BtsCI GGATG 6 cut(s) 197, 675, 879, 1089, 1213, 1321
BtsIMutI CAGTG 2 cut(s) 14, 309
BveI ACCTGC 1 cut(s) 625
Cac8I GCNNGC 8 cut(s) 182, 601, 680, 684, 966, 1219, 1275, 1288
CaiI CAGNNNCTG 1 cut(s) 892
CfoI GCGC 4 cut(s) 180, 218, 551, 1226
Cfr13I GGNCC 3 cut(s) 412, 895, 1054
CseI GACGC 2 cut(s) 413, 889
CsiI ACCWGGT 1 cut(s) 338
Csp6I GTAC 4 cut(s) 225, 258, 614, 780
CviQI GTAC 4 cut(s) 225, 258, 614, 780
DdeI CTNAG 5 cut(s) 699, 718, 793, 1227, 1380
DpnI GATC 6 cut(s) 27, 348, 515, 637, 863, 1266
DpnII GATC 6 cut(s) 25, 346, 513, 635, 861, 1264
DrdI GACNNNNNNGTC 1 cut(s) 410
DseDI GACNNNNNNGTC 1 cut(s) 410
EaeI YGGCCR 4 cut(s) 145, 153, 1138, 1335
EagI CGGCCG 1 cut(s) 1138
Eam1104I CTCTTC 1 cut(s) 72
EarI CTCTTC 1 cut(s) 72
EclXI CGGCCG 1 cut(s) 1138
Eco130I CCWWGG 3 cut(s) 399, 1194, 1311
Eco47I GGWCC 2 cut(s) 412, 895
Eco47III AGCGCT 1 cut(s) 550
Eco52I CGGCCG 1 cut(s) 1138
Eco72I CACGTG 1 cut(s) 611
Eco88I CYCGRG 2 cut(s) 531, 626
EcoRII CCWGG 4 cut(s) 147, 200, 338, 481
EcoT14I CCWWGG 3 cut(s) 399, 1194, 1311
ErhI CCWWGG 3 cut(s) 399, 1194, 1311
FalI AAGNNNNNCTT 4 cut(s) 269, 301, 675, 707
FaqI GGGAC 4 cut(s) 218, 398, 881, 1145
FauI CCCGC 2 cut(s) 584, 592
FbaI TGATCA 2 cut(s) 346, 513
FblI GTMKAC 3 cut(s) 172, 733, 1307
FokI GGATG 6 cut(s) 184, 682, 886, 1096, 1220, 1328
FspBI CTAG 1 cut(s) 1374
GlaI GCGC 4 cut(s) 179, 217, 550, 1225
GsaI CCCAGC 3 cut(s) 21, 25, 645
GsuI CTGGAG 1 cut(s) 251
HaeII RGCGCY 2 cut(s) 552, 1227
HaeIII GGCC 5 cut(s) 147, 155, 1055, 1140, 1337
HapII CCGG 2 cut(s) 372, 1166
HgaI GACGC 2 cut(s) 413, 889
HhaI GCGC 4 cut(s) 180, 218, 551, 1226
Hin6I GCGC 4 cut(s) 178, 216, 549, 1224
HinP1I GCGC 4 cut(s) 178, 216, 549, 1224
HincII GTYRAC 2 cut(s) 244, 1308
HindII GTYRAC 2 cut(s) 244, 1308
HindIII AAGCTT 1 cut(s) 921
HinfI GANTC 5 cut(s) 233, 464, 486, 716, 1158
HpaII CCGG 2 cut(s) 372, 1166
HphI GGTGA 1 cut(s) 1166
Hpy166II GTNNAC 5 cut(s) 173, 244, 614, 734, 1308
Hpy188I TCNGA 1 cut(s) 841
Hpy188III TCNNGA 3 cut(s) 29, 95, 230
Hpy8I GTNNAC 5 cut(s) 173, 244, 614, 734, 1308
Hpy99I CGWCG 2 cut(s) 360, 758
HpyAV CCTTC 1 cut(s) 195
HpyCH4III ACNGT 6 cut(s) 38, 449, 478, 713, 731, 1255
HpyCH4IV ACGT 3 cut(s) 610, 662, 844
HpyF10VI GCNNNNNNNGC 6 cut(s) 501, 605, 889, 1218, 1223, 1283
HpyF3I CTNAG 5 cut(s) 699, 718, 793, 1227, 1380
HpySE526I ACGT 3 cut(s) 610, 662, 844
HspAI GCGC 4 cut(s) 178, 216, 549, 1224
Ksp22I TGATCA 2 cut(s) 346, 513
Kzo9I GATC 6 cut(s) 25, 346, 513, 635, 861, 1264
LmnI GCTCC 3 cut(s) 97, 382, 530
Lsp1109I GCAGC 8 cut(s) 558, 805, 808, 870, 1039, 1228, 1309, 1379
LweI GCATC 4 cut(s) 206, 1248, 1306, 1379
MabI ACCWGGT 1 cut(s) 338
MaeI CTAG 1 cut(s) 1374
MaeII ACGT 3 cut(s) 610, 662, 844
MaeIII GTNAC 4 cut(s) 334, 713, 725, 1233
MalI GATC 6 cut(s) 27, 348, 515, 637, 863, 1266
MbiI CCGCTC 2 cut(s) 92, 651
MboI GATC 6 cut(s) 25, 346, 513, 635, 861, 1264
MboII GAAGA 3 cut(s) 59, 889, 1163
MfeI CAATTG 1 cut(s) 959
MflI RGATCY 2 cut(s) 25, 635
MhlI GDGCHC 2 cut(s) 379, 527
MlsI TGGCCA 2 cut(s) 147, 155
MluCI AATT 4 cut(s) 82, 901, 959, 1349
MluI ACGCGT 1 cut(s) 405
MluNI TGGCCA 2 cut(s) 147, 155
MlyI GAGTC 5 cut(s) 242, 458, 495, 710, 1167
MmeI TCCRAC 2 cut(s) 90, 408
Mox20I TGGCCA 2 cut(s) 147, 155
MscI TGGCCA 2 cut(s) 147, 155
MslI CAYNNNNRTG 5 cut(s) 392, 398, 509, 533, 628
Msp20I TGGCCA 2 cut(s) 147, 155
MspA1I CMGCKG 1 cut(s) 21
MspI CCGG 2 cut(s) 372, 1166
MspR9I CCNGG 4 cut(s) 149, 202, 340, 483
MunI CAATTG 1 cut(s) 959
MvaI CCWGG 4 cut(s) 149, 202, 340, 483
MvnI CGCG 4 cut(s) 178, 216, 407, 557
MwoI GCNNNNNNNGC 6 cut(s) 501, 605, 889, 1218, 1223, 1283
NcoI CCATGG 3 cut(s) 399, 1194, 1311
NdeII GATC 6 cut(s) 25, 346, 513, 635, 861, 1264
NlaIV GGNNCC 3 cut(s) 414, 897, 1056
NmeAIII GCCGAG 2 cut(s) 81, 471
NmuCI GTSAC 3 cut(s) 334, 713, 725
NspI RCATGY 1 cut(s) 1290
OliI CACNNNNGTG 2 cut(s) 533, 628
PaeI GCATGC 1 cut(s) 1290
PaeR7I CTCGAG 1 cut(s) 531
PcsI WCGNNNNNNNCGW 1 cut(s) 53
PleI GAGTC 5 cut(s) 241, 458, 494, 710, 1166
PmaCI CACGTG 1 cut(s) 611
PmlI CACGTG 1 cut(s) 611
PpsI GAGTC 5 cut(s) 241, 458, 494, 710, 1166
Ppu21I YACGTR 1 cut(s) 611
Psp6I CCWGG 4 cut(s) 147, 200, 338, 481
PspCI CACGTG 1 cut(s) 611
PspFI CCCAGC 3 cut(s) 17, 21, 641
PspGI CCWGG 4 cut(s) 147, 200, 338, 481
PspN4I GGNNCC 3 cut(s) 414, 897, 1056
PspPI GGNCC 3 cut(s) 412, 895, 1054
PspXI VCTCGAGB 1 cut(s) 531
PstI CTGCAG 1 cut(s) 888
PstNI CAGNNNCTG 1 cut(s) 892
PsuI RGATCY 2 cut(s) 25, 635
PvuII CAGCTG 1 cut(s) 21
RsaI GTAC 4 cut(s) 226, 259, 615, 781
RsaNI GTAC 4 cut(s) 225, 258, 614, 780
RseI CAYNNNNRTG 5 cut(s) 392, 398, 509, 533, 628
SalI GTCGAC 1 cut(s) 1306
Sau3AI GATC 6 cut(s) 25, 346, 513, 635, 861, 1264
Sau96I GGNCC 3 cut(s) 412, 895, 1054
ScaI AGTACT 1 cut(s) 781
SchI GAGTC 5 cut(s) 242, 458, 495, 710, 1167
ScrFI CCNGG 4 cut(s) 149, 202, 340, 483
SduI GDGCHC 2 cut(s) 379, 527
SexAI ACCWGGT 1 cut(s) 338
SfaNI GCATC 4 cut(s) 206, 1248, 1306, 1379
SfcI CTRYAG 2 cut(s) 468, 884
Sfr274I CTCGAG 1 cut(s) 531
SinI GGWCC 2 cut(s) 412, 895
SlaI CTCGAG 1 cut(s) 531
SmiMI CAYNNNNRTG 5 cut(s) 392, 398, 509, 533, 628
SmlI CTYRAG 2 cut(s) 531, 969
SmoI CTYRAG 2 cut(s) 531, 969
SphI GCATGC 1 cut(s) 1290
Sse9I AATT 4 cut(s) 82, 901, 959, 1349
SspMI CTAG 1 cut(s) 1374
StyD4I CCNGG 4 cut(s) 147, 200, 338, 481
StyI CCWWGG 3 cut(s) 399, 1194, 1311
TaaI ACNGT 6 cut(s) 38, 449, 478, 713, 731, 1255
TaiI ACGT 3 cut(s) 613, 665, 847
TaqI TCGA 2 cut(s) 532, 1307
TasI AATT 4 cut(s) 82, 901, 959, 1349
TatI WGTACW 2 cut(s) 257, 779
TauI GCSGC 3 cut(s) 1143, 1224, 1337
TscAI CASTG 2 cut(s) 14, 316
TseFI GTSAC 3 cut(s) 334, 713, 725
TseI GCWGC 8 cut(s) 546, 818, 821, 883, 1027, 1241, 1297, 1367
Tsp45I GTSAC 3 cut(s) 334, 713, 725
TspDTI ATGAA 5 cut(s) 453, 890, 1007, 1142, 1191
TspGWI ACGGA 1 cut(s) 344
TspRI CASTG 2 cut(s) 14, 316
VpaK11BI GGWCC 2 cut(s) 412, 895
XapI RAATTY 1 cut(s) 901
XceI RCATGY 1 cut(s) 1290
XcmI CCANNNNNNNNNTGG 4 cut(s) 163, 397, 406, 667
XhoI CTCGAG 1 cut(s) 531
XmiI GTMKAC 3 cut(s) 172, 733, 1307
XspI CTAG 1 cut(s) 1374
ZrmI AGTACT 1 cut(s) 781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.