Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0012487

Physical Location & Seq

Genomic Coordinates Reverse (-)

24259987 .. 24264149

Gene Structure 4163 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1161400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1539 bp

ATGGTGACCTCCTTTCCTGCTTCTCTCTCTCTAAATCCCTCTCTCTCTCCTCCTCCCTTTTCTGCAACCAATACCCAATTATCCTTCTCCTCGCCCTTTCCTCCTCTCAGATTCCCAGCCTGCCGATCCTCACGAACAAAACCCAGTAGCCTTTGCGGTAAAGGTGTTCGATTGTGTTGCTCTGAGAGACCAAAAAGTAGAAAACTTGAGCTTAAAGCCAGCGACATGACTATTACAGAGGTCGGGCAAGATGAAGAGGAAGAGGGTCCTCCTCCATATGTTGAGTCGGAGATTAACTCGAGACCTCGACGCATTGCTCTCTTTGTTGAGCCTTCTCCCTTTGCATATGTGTCAGGTTATAAAAATCGGTTCCAGAATTTCATTCGATACTTGCGTGAAATGGGGGATGAGGTGATGGTTGTGACAACCCATGAAGGAGTTCCTGACGAATTTTATGGAGCAAAGTTGATTGGATCCCGAAGCTTTCCCTTCCCCTGGTATCAAAAGGTGCCCCTTTCCCTTGCACTTAGTCCTAGAATAATTTCGGAGGTTGCTCAGTTCAAGCCTGACATTATACATGCATCCTCGCCTGGAATTATGGTTTTCGGCGCCCTCATTATCGCTAAGTTGTTATGTGTTCCTATAGTGATGTCGTATCACACTCATGTGCCAGTATATATCCCGAGATACACCTTCAGTTGGCTGGTGAAACCCATGTGGTTGGTCATAAAGTTTCTGCACAGAGCTGCTGATCTCACACTAGTGCCATCAGCTGCAATCAGTAAGGATCTCCTAGAAGCTGGGGTGGCCGCAGCTAATACAATCCGTCTTTGGAATAAGGGTGTTGATTCGGAAAGCTTCCATCCCCGCTTCCGTTCTCATGAAATGCGTATGAGACTGAGCAACGGTGAACCTGAGAAACCTTTAATTGTTCATGTTGGAAGACTTGGAGTGGAGAAGAGTTTGGATTTTCTGAAAAGTGTCATGGATCGGCTTCCTGAAGCACGAATTGCTTTCATTGGAGATGGACCATACAGGGAGGATCTTGAAAAACTGTTTGATGGCATGCCTGCAGTATTTACAGGAATGTTAGGAGGTGAAGAGCTCTCACAGGCATATGCCAGTGGTGACGTTTTTGTGATGCCTTCAGAATCAGAGACACTTGGGCTTGTTGTATTGGAGGCTATGTCTTCAGGTATCCCTGTGGTGGGAGCGCGAGCTGGTGGAATCCCAGATATAATTCCCACAGATCAGGAGGGGAAAACTGGCTTTCTCTACGATTATAATGACCTTGATGACTGCTTAAGTAAGTTACAGCCCCTGCTGGAGAACAAGGACTTAAGGGAAACAATTGGCAAGGCAGCACGTGAGGAAATGGAGAAGTACGATTGGAAAGCAGCCACGCGAGTAATTCGAAATGAGCAATACAATGCTGCCATTTGGTTCTGGAGGAAGAAGAGAGCGCAATTTCTTAGACCTCTGCAGTGGTTCATGAAGCGCATTTTTCCTCCAACCACACCAGCAATCAAGTACAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000030 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006643 GO:0006664 GO:0006790 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008194 GO:0008610 GO:0009058 GO:0009247 GO:0009267 GO:0009507 GO:0009526 GO:0009536 GO:0009605 GO:0009941 GO:0009987 GO:0009991 GO:0016036 GO:0016740 GO:0016757 GO:0016758 GO:0031667 GO:0031668 GO:0031669 GO:0031967 GO:0031975 GO:0033554 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046467 GO:0046505 GO:0046506 GO:0050896 GO:0051716 GO:0070085 GO:0071496 GO:0071704 GO:0097502 GO:1901135 GO:1901137 GO:1901576 GO:1903509

KEGG Pathways

Metabolic & Signaling

ko00561 ko01100 map00561 map01100

Pfam Domains

Protein Families

Glyco_trans_1_4 Glyco_transf_4 Glycos_transf_1

Protein Analysis

513

Amino Acids

57.61

Weight (kDa)

8.69

Isoelectric Point (pI)

48.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_4	PF13439	119 - 285	1.7e-27	Glycosyltransferase Family 4
Glyco_trans_4_4	PF13579	119 - 274	1.1e-16	Glycosyl transferase 4-like domain
Glycos_transf_1	PF00534	305 - 457	4.4e-27	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	308 - 444	5.8e-26	Glycosyl transferases group 1
Glyco_trans_1_2	PF13524	324 - 464	8.6e-10	Glycosyl transferase-like
GT4-conflict	PF20706	367 - 469	7.2e-08	Family 4 Glycosyltransferase in conflict systems

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012487)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01220 AT5G01220
fragaria_vesca	FvH4_6g11930
malus_domestica	MD04G1148000.v1.1 MD12G1161400.v1.1
prunus_persica	Prupe.6G271800_v2.0.a1
pyrus_communis	pycom04g13380 pycom12g15320
rosa_chinensis	RchiOBHm_Chr3g0463701
rosa_laevigata	RLG00000024763
rosa_multiflora	Rmu_sc0003267.1_g000033
rosa_roxburghii	Rroxscaffold_6G00416360
rosa_rugosa	Rorug03G0067000 Rorug03G0067100
rosa_samantha	Rh3AG124100 Rh3BG128700 Rh3CG130900 Rh3DG129300
rosa_wichuraiana	Rw3G010490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	360, 1284
AccB1I	GGYRCC	2 cut(s)	508, 608
AccII	CGCG	2 cut(s)	1216, 1405
AciI	CCGC	3 cut(s)	156, 810, 868
AclWI	GGATC	6 cut(s)	120, 468, 481, 795, 996, 1050
AcoI	YGGCCR	1 cut(s)	807
AcsI	RAATTY	2 cut(s)	376, 449
AcuI	CTGAAG	4 cut(s)	679, 1020, 1131, 1176
AcvI	CACGTG	1 cut(s)	1367
AcyI	GRCGYC	1 cut(s)	609
AfaI	GTAC	2 cut(s)	1385, 1532
AfiI	CCNNNNNNNGG	4 cut(s)	495, 699, 1207, 1208
AflII	CTTAAG	2 cut(s)	1303, 1339
AgsI	TTSAA	2 cut(s)	562, 1049
AhlI	ACTAGT	1 cut(s)	760
AjnI	CCWGG	2 cut(s)	494, 589
AleI	CACNNNNGTG	2 cut(s)	665, 761
AluBI	AGCT	9 cut(s)	211, 483, 746, 773, 800, 815, 858, 1105, 1220
AluI	AGCT	9 cut(s)	211, 483, 746, 773, 800, 815, 858, 1105, 1220
Alw21I	GWGCWC	1 cut(s)	1107
Alw26I	GTCTC	4 cut(s)	181, 295, 889, 1151
AlwI	GGATC	6 cut(s)	120, 468, 481, 795, 996, 1050
AlwNI	CAGNNNCTG	1 cut(s)	1321
Ama87I	CYCGRG	2 cut(s)	298, 682
AoxI	GGCC	1 cut(s)	807
ApeKI	GCWGC	6 cut(s)	746, 773, 812, 1361, 1397, 1433
ApoI	RAATTY	2 cut(s)	376, 449
Asp700I	GAANNNNTTC	2 cut(s)	438, 541
AspLEI	GCGC	4 cut(s)	611, 1216, 1465, 1500
AspS9I	GGNCC	2 cut(s)	266, 1028
AsuHPI	GGTGA	6 cut(s)	16, 424, 718, 920, 1109, 1139
AsuII	TTCGAA	1 cut(s)	1414
AvaI	CYCGRG	2 cut(s)	298, 682
AvaII	GGWCC	2 cut(s)	266, 1028
BaeGI	GKGCMC	1 cut(s)	513
BaeI	ACNNNNGTAYC	2 cut(s)	379, 412
BamHI	GGATCC	1 cut(s)	473
BanI	GGYRCC	2 cut(s)	508, 608
BanII	GRGCYC	1 cut(s)	1107
BbrPI	CACGTG	1 cut(s)	1367
BbsI	GAAGAC	2 cut(s)	949, 1182
Bbv12I	GWGCWC	1 cut(s)	1107
BbvI	GCAGC	6 cut(s)	733, 760, 824, 1373, 1409, 1420
BccI	CCATC	5 cut(s)	409, 775, 870, 1019, 1055
BcgI	CGANNNNNNTGC	2 cut(s)	159, 193
BciT130I	CCWGG	2 cut(s)	496, 591
BciVI	GTATCC	1 cut(s)	1208
BcoDI	GTCTC	4 cut(s)	181, 295, 889, 1151
BcuI	ACTAGT	1 cut(s)	760
BfaI	CTAG	3 cut(s)	534, 761, 794
BfmI	CTRYAG	3 cut(s)	642, 1071, 1481
BfoI	RGCGCY	1 cut(s)	612
BfrI	CTTAAG	2 cut(s)	1303, 1339
BfuI	GTATCC	1 cut(s)	1208
BisI	GCNGC	7 cut(s)	747, 774, 810, 813, 1362, 1398, 1434
BlsI	GCNGC	7 cut(s)	748, 775, 811, 814, 1363, 1399, 1435
Bme1390I	CCNGG	2 cut(s)	496, 591
Bme18I	GGWCC	2 cut(s)	266, 1028
BmeT110I	CYCGRG	2 cut(s)	298, 682
BmgT120I	GGNCC	2 cut(s)	266, 1028
BmiI	GGNNCC	5 cut(s)	267, 371, 475, 510, 610
BmrFI	CCNGG	2 cut(s)	496, 591
BmrI	ACTGGG	1 cut(s)	138
BmsI	GCATC	2 cut(s)	590, 1131
BmuI	ACTGGG	1 cut(s)	138
BpiI	GAAGAC	2 cut(s)	949, 1182
BplI	GAGNNNNNCTC	2 cut(s)	281, 313
BpmI	CTGGAG	2 cut(s)	1346, 1468
Bpu14I	TTCGAA	1 cut(s)	1414
BpuEI	CTTGAG	1 cut(s)	227
BsaAI	YACGTR	1 cut(s)	1367
BsaHI	GRCGYC	1 cut(s)	609
BsaI	GGTCTC	2 cut(s)	181, 295
BsaJI	CCNNGG	1 cut(s)	494
BsaXI	ACNNNNNCTCC	2 cut(s)	1172, 1202
Bsc4I	CCNNNNNNNGG	4 cut(s)	495, 699, 1207, 1208
Bse1I	ACTGG	4 cut(s)	144, 671, 1122, 1270
Bse3DI	GCAATG	1 cut(s)	312
BseBI	CCWGG	2 cut(s)	496, 591
BseDI	CCNNGG	1 cut(s)	494
BseGI	GGATG	3 cut(s)	412, 581, 862
BseLI	CCNNNNNNNGG	4 cut(s)	495, 699, 1207, 1208
BseMI	GCAATG	1 cut(s)	312
BseMII	CTCAG	5 cut(s)	121, 174, 569, 890, 906
BseNI	ACTGG	4 cut(s)	144, 671, 1122, 1270
BseRI	GAGGAG	5 cut(s)	39, 42, 79, 93, 261
BseSI	GKGCMC	1 cut(s)	513
BseXI	GCAGC	6 cut(s)	733, 760, 824, 1373, 1409, 1420
BseYI	CCCAGC	2 cut(s)	115, 800
BsgI	GTGCAG	1 cut(s)	722
Bsh1236I	CGCG	2 cut(s)	1216, 1405
BshFI	GGCC	1 cut(s)	809
BshNI	GGYRCC	2 cut(s)	508, 608
BsiHKAI	GWGCWC	1 cut(s)	1107
BsiHKCI	CYCGRG	2 cut(s)	298, 682
BslI	CCNNNNNNNGG	4 cut(s)	495, 699, 1207, 1208
BsmAI	GTCTC	4 cut(s)	181, 295, 889, 1151
BsnI	GGCC	1 cut(s)	809
Bso31I	GGTCTC	2 cut(s)	181, 295
BsoBI	CYCGRG	2 cut(s)	298, 682
Bsp119I	TTCGAA	1 cut(s)	1414
Bsp1286I	GDGCHC	2 cut(s)	513, 1107
Bsp143I	GATC	7 cut(s)	125, 473, 751, 787, 988, 1042, 1249
BspACI	CCGC	3 cut(s)	156, 810, 868
BspANI	GGCC	1 cut(s)	809
BspCNI	CTCAG	5 cut(s)	120, 175, 568, 891, 907
BspFNI	CGCG	2 cut(s)	1216, 1405
BspHI	TCATGA	2 cut(s)	880, 1491
BspLI	GGNNCC	5 cut(s)	267, 371, 475, 510, 610
BspMAI	CTGCAG	2 cut(s)	1075, 1485
BspPI	GGATC	6 cut(s)	120, 468, 481, 795, 996, 1050
BspQI	GCTCTTC	1 cut(s)	1095
BspT104I	TTCGAA	1 cut(s)	1414
BspT107I	GGYRCC	2 cut(s)	508, 608
BspTI	CTTAAG	2 cut(s)	1303, 1339
BspTNI	GGTCTC	2 cut(s)	181, 295
BsrDI	GCAATG	1 cut(s)	312
BsrI	ACTGG	4 cut(s)	144, 671, 1122, 1270
BssECI	CCNNGG	1 cut(s)	494
BssMI	GATC	7 cut(s)	125, 473, 751, 787, 988, 1042, 1249
BssNI	GRCGYC	1 cut(s)	609
Bst2UI	CCWGG	2 cut(s)	496, 591
Bst4CI	ACNGT	2 cut(s)	908, 1056
Bst6I	CTCTTC	5 cut(s)	249, 255, 953, 1095, 1451
BstACI	GRCGYC	1 cut(s)	609
BstAFI	CTTAAG	2 cut(s)	1303, 1339
BstAPI	GCANNNNNTGC	1 cut(s)	1010
BstBAI	YACGTR	1 cut(s)	1367
BstBI	TTCGAA	1 cut(s)	1414
BstC8I	GCNNGC	5 cut(s)	121, 220, 1067, 1071, 1218
BstDEI	CTNAG	8 cut(s)	107, 183, 527, 555, 624, 899, 915, 1472
BstEII	GGTNACC	1 cut(s)	4
BstF5I	GGATG	3 cut(s)	412, 581, 862
BstFNI	CGCG	2 cut(s)	1216, 1405
BstH2I	RGCGCY	1 cut(s)	612
BstHHI	GCGC	4 cut(s)	611, 1216, 1465, 1500
BstKTI	GATC	7 cut(s)	128, 476, 754, 790, 991, 1045, 1252
BstMAI	GTCTC	4 cut(s)	181, 295, 889, 1151
BstMBI	GATC	7 cut(s)	125, 473, 751, 787, 988, 1042, 1249
BstMWI	GCNNNNNNNGC	2 cut(s)	806, 1010
BstNI	CCWGG	2 cut(s)	496, 591
BstNSI	RCATGY	2 cut(s)	581, 1069
BstPI	GGTNACC	1 cut(s)	4
BstSCI	CCNGG	2 cut(s)	494, 589
BstSFI	CTRYAG	3 cut(s)	642, 1071, 1481
BstSLI	GKGCMC	1 cut(s)	513
BstUI	CGCG	2 cut(s)	1216, 1405
BstV1I	GCAGC	6 cut(s)	733, 760, 824, 1373, 1409, 1420
BstV2I	GAAGAC	2 cut(s)	949, 1182
BstX2I	RGATCY	3 cut(s)	473, 787, 1042
BstXI	CCANNNNNNTGG	1 cut(s)	721
BstYI	RGATCY	3 cut(s)	473, 787, 1042
BsuI	GTATCC	1 cut(s)	1208
BsuRI	GGCC	1 cut(s)	809
BtsCI	GGATG	3 cut(s)	412, 581, 862
BtsI	GCAGTG	1 cut(s)	1490
BtsIMutI	CAGTG	2 cut(s)	1129, 1490
Cac8I	GCNNGC	5 cut(s)	121, 220, 1067, 1071, 1218
CaiI	CAGNNNCTG	1 cut(s)	1321
CciI	TCATGA	2 cut(s)	880, 1491
CfoI	GCGC	4 cut(s)	611, 1216, 1465, 1500
Cfr13I	GGNCC	2 cut(s)	266, 1028
CseI	GACGC	1 cut(s)	318
Csp6I	GTAC	2 cut(s)	1384, 1531
CviQI	GTAC	2 cut(s)	1384, 1531
DdeI	CTNAG	8 cut(s)	107, 183, 527, 555, 624, 899, 915, 1472
DinI	GGCGCC	1 cut(s)	610
DpnI	GATC	7 cut(s)	127, 475, 753, 789, 990, 1044, 1251
DpnII	GATC	7 cut(s)	125, 473, 751, 787, 988, 1042, 1249
EaeI	YGGCCR	1 cut(s)	807
Eam1104I	CTCTTC	5 cut(s)	249, 255, 953, 1095, 1451
EarI	CTCTTC	5 cut(s)	249, 255, 953, 1095, 1451
Ecl136II	GAGCTC	1 cut(s)	1105
Eco24I	GRGCYC	1 cut(s)	1107
Eco31I	GGTCTC	2 cut(s)	181, 295
Eco47I	GGWCC	2 cut(s)	266, 1028
Eco53kI	GAGCTC	1 cut(s)	1105
Eco57I	CTGAAG	4 cut(s)	679, 1020, 1131, 1176
Eco72I	CACGTG	1 cut(s)	1367
Eco88I	CYCGRG	2 cut(s)	298, 682
Eco91I	GGTNACC	1 cut(s)	4
EcoICRI	GAGCTC	1 cut(s)	1105
EcoO109I	RGGNCCY	1 cut(s)	266
EcoO65I	GGTNACC	1 cut(s)	4
EcoRII	CCWGG	2 cut(s)	494, 589
EcoT22I	ATGCAT	1 cut(s)	583
EcoT38I	GRGCYC	1 cut(s)	1107
EgeI	GGCGCC	1 cut(s)	610
EheI	GGCGCC	1 cut(s)	610
FauI	CCCGC	1 cut(s)	875
FauNDI	CATATG	3 cut(s)	277, 346, 1117
Fnu4HI	GCNGC	7 cut(s)	747, 774, 810, 813, 1362, 1398, 1434
FokI	GGATG	3 cut(s)	419, 568, 849
FriOI	GRGCYC	1 cut(s)	1107
Fsp4HI	GCNGC	7 cut(s)	747, 774, 810, 813, 1362, 1398, 1434
FspBI	CTAG	3 cut(s)	534, 761, 794
GlaI	GCGC	4 cut(s)	610, 1215, 1464, 1499
GluI	GCNGC	7 cut(s)	747, 774, 810, 813, 1362, 1398, 1434
GsaI	CCCAGC	2 cut(s)	119, 804
GsuI	CTGGAG	2 cut(s)	1346, 1468
HaeII	RGCGCY	1 cut(s)	612
HaeIII	GGCC	1 cut(s)	809
HgaI	GACGC	1 cut(s)	318
HhaI	GCGC	4 cut(s)	611, 1216, 1465, 1500
Hin1I	GRCGYC	1 cut(s)	609
Hin6I	GCGC	4 cut(s)	609, 1214, 1463, 1498
HinP1I	GCGC	4 cut(s)	609, 1214, 1463, 1498
HindIII	AAGCTT	2 cut(s)	481, 856
HinfI	GANTC	5 cut(s)	111, 284, 848, 1151, 1227
HphI	GGTGA	6 cut(s)	16, 424, 718, 920, 1109, 1139
Hpy166II	GTNNAC	1 cut(s)	911
Hpy188I	TCNGA	8 cut(s)	110, 184, 289, 547, 853, 975, 1150, 1156
Hpy8I	GTNNAC	1 cut(s)	911
Hpy99I	CGWCG	1 cut(s)	312
HpyAV	CCTTC	6 cut(s)	94, 342, 428, 499, 703, 1155
HpyCH4III	ACNGT	2 cut(s)	908, 1056
HpyCH4IV	ACGT	2 cut(s)	1131, 1366
HpyCH4V	TGCA	8 cut(s)	65, 344, 524, 581, 739, 776, 1073, 1483
HpyF10VI	GCNNNNNNNGC	2 cut(s)	806, 1010
HpyF3I	CTNAG	8 cut(s)	107, 183, 527, 555, 624, 899, 915, 1472
HpySE526I	ACGT	2 cut(s)	1131, 1366
Hsp92I	GRCGYC	1 cut(s)	609
HspAI	GCGC	4 cut(s)	609, 1214, 1463, 1498
KasI	GGCGCC	1 cut(s)	608
Kzo9I	GATC	7 cut(s)	125, 473, 751, 787, 988, 1042, 1249
LguI	GCTCTTC	1 cut(s)	1095
LmnI	GCTCC	2 cut(s)	458, 1211
Lsp1109I	GCAGC	6 cut(s)	733, 760, 824, 1373, 1409, 1420
LweI	GCATC	2 cut(s)	590, 1131
MaeI	CTAG	3 cut(s)	534, 761, 794
MaeII	ACGT	2 cut(s)	1131, 1366
MaeIII	GTNAC	4 cut(s)	4, 421, 1127, 1311
MalI	GATC	7 cut(s)	127, 475, 753, 789, 990, 1044, 1251
MboI	GATC	7 cut(s)	125, 473, 751, 787, 988, 1042, 1249
MboII	GAAGA	8 cut(s)	266, 272, 954, 970, 1112, 1182, 1465, 1468
MfeI	CAATTG	1 cut(s)	1350
MflI	RGATCY	3 cut(s)	473, 787, 1042
MhlI	GDGCHC	2 cut(s)	513, 1107
Mly113I	GGCGCC	1 cut(s)	609
MlyI	GAGTC	1 cut(s)	293
MmeI	TCCRAC	3 cut(s)	267, 919, 1535
Mph1103I	ATGCAT	1 cut(s)	583
MroXI	GAANNNNTTC	2 cut(s)	438, 541
MseI	TTAA	5 cut(s)	213, 294, 926, 1304, 1340
MslI	CAYNNNNRTG	3 cut(s)	663, 665, 761
MspA1I	CMGCKG	1 cut(s)	773
MspCI	CTTAAG	2 cut(s)	1303, 1339
MspR9I	CCNGG	2 cut(s)	496, 591
MunI	CAATTG	1 cut(s)	1350
MvaI	CCWGG	2 cut(s)	496, 591
MvnI	CGCG	2 cut(s)	1216, 1405
MwoI	GCNNNNNNNGC	2 cut(s)	806, 1010
NarI	GGCGCC	1 cut(s)	609
NdeI	CATATG	3 cut(s)	277, 346, 1117
NdeII	GATC	7 cut(s)	125, 473, 751, 787, 988, 1042, 1249
NlaIV	GGNNCC	5 cut(s)	267, 371, 475, 510, 610
NmuCI	GTSAC	3 cut(s)	4, 421, 1127
NsiI	ATGCAT	1 cut(s)	583
NspI	RCATGY	2 cut(s)	581, 1069
NspV	TTCGAA	1 cut(s)	1414
OliI	CACNNNNGTG	2 cut(s)	665, 761
PaeI	GCATGC	1 cut(s)	1069
PaeR7I	CTCGAG	1 cut(s)	298
PagI	TCATGA	2 cut(s)	880, 1491
PciSI	GCTCTTC	1 cut(s)	1095
PcsI	WCGNNNNNNNCGW	1 cut(s)	391
PdmI	GAANNNNTTC	2 cut(s)	438, 541
PfeI	GAWTC	4 cut(s)	111, 848, 1151, 1227
PkrI	GCNGC	7 cut(s)	748, 775, 811, 814, 1363, 1399, 1435
PleI	GAGTC	1 cut(s)	292
PluTI	GGCGCC	1 cut(s)	612
PmaCI	CACGTG	1 cut(s)	1367
PmlI	CACGTG	1 cut(s)	1367
PpsI	GAGTC	1 cut(s)	292
Ppu21I	YACGTR	1 cut(s)	1367
PpuMI	RGGWCCY	1 cut(s)	266
PsiI	TTATAA	2 cut(s)	360, 1284
Psp124BI	GAGCTC	1 cut(s)	1107
Psp5II	RGGWCCY	1 cut(s)	266
Psp6I	CCWGG	2 cut(s)	494, 589
PspCI	CACGTG	1 cut(s)	1367
PspEI	GGTNACC	1 cut(s)	4
PspFI	CCCAGC	2 cut(s)	115, 800
PspGI	CCWGG	2 cut(s)	494, 589
PspN4I	GGNNCC	5 cut(s)	267, 371, 475, 510, 610
PspPI	GGNCC	2 cut(s)	266, 1028
PspPPI	RGGWCCY	1 cut(s)	266
PstI	CTGCAG	2 cut(s)	1075, 1485
PstNI	CAGNNNCTG	1 cut(s)	1321
PsuI	RGATCY	3 cut(s)	473, 787, 1042
PvuII	CAGCTG	1 cut(s)	773
RsaI	GTAC	2 cut(s)	1385, 1532
RsaNI	GTAC	2 cut(s)	1384, 1531
RseI	CAYNNNNRTG	3 cut(s)	663, 665, 761
SacI	GAGCTC	1 cut(s)	1107
SapI	GCTCTTC	1 cut(s)	1095
SaqAI	TTAA	5 cut(s)	213, 294, 926, 1304, 1340
SatI	GCNGC	7 cut(s)	747, 774, 810, 813, 1362, 1398, 1434
Sau3AI	GATC	7 cut(s)	125, 473, 751, 787, 988, 1042, 1249
Sau96I	GGNCC	2 cut(s)	266, 1028
SchI	GAGTC	1 cut(s)	293
ScrFI	CCNGG	2 cut(s)	496, 591
SduI	GDGCHC	2 cut(s)	513, 1107
SfaNI	GCATC	2 cut(s)	590, 1131
SfcI	CTRYAG	3 cut(s)	642, 1071, 1481
SfoI	GGCGCC	1 cut(s)	610
Sfr274I	CTCGAG	1 cut(s)	298
SfuI	TTCGAA	1 cut(s)	1414
SinI	GGWCC	2 cut(s)	266, 1028
SlaI	CTCGAG	1 cut(s)	298
SmiMI	CAYNNNNRTG	3 cut(s)	663, 665, 761
SmlI	CTYRAG	4 cut(s)	206, 298, 1303, 1339
SmoI	CTYRAG	4 cut(s)	206, 298, 1303, 1339
SpeI	ACTAGT	1 cut(s)	760
SphI	GCATGC	1 cut(s)	1069
SsiI	CCGC	3 cut(s)	156, 810, 868
SspDI	GGCGCC	1 cut(s)	608
SspMI	CTAG	3 cut(s)	534, 761, 794
SstI	GAGCTC	1 cut(s)	1107
StyD4I	CCNGG	2 cut(s)	494, 589
TaaI	ACNGT	2 cut(s)	908, 1056
TaiI	ACGT	2 cut(s)	1134, 1369
TaqI	TCGA	5 cut(s)	169, 299, 307, 385, 1414
TatI	WGTACW	1 cut(s)	1530
TauI	GCSGC	1 cut(s)	812
TfiI	GAWTC	4 cut(s)	111, 848, 1151, 1227
Tru1I	TTAA	5 cut(s)	213, 294, 926, 1304, 1340
Tru9I	TTAA	5 cut(s)	213, 294, 926, 1304, 1340
TscAI	CASTG	2 cut(s)	1129, 1490
TseFI	GTSAC	3 cut(s)	4, 421, 1127
TseI	GCWGC	6 cut(s)	746, 773, 812, 1361, 1397, 1433
Tsp45I	GTSAC	3 cut(s)	4, 421, 1127
TspDTI	ATGAA	8 cut(s)	267, 370, 447, 897, 923, 1006, 1480, 1508
TspGWI	ACGGA	2 cut(s)	815, 863
TspRI	CASTG	2 cut(s)	1129, 1490
Vha464I	CTTAAG	2 cut(s)	1303, 1339
VpaK11BI	GGWCC	2 cut(s)	266, 1028
XapI	RAATTY	2 cut(s)	376, 449
XceI	RCATGY	2 cut(s)	581, 1069
XhoI	CTCGAG	1 cut(s)	298
XmnI	GAANNNNTTC	2 cut(s)	438, 541
XspI	CTAG	3 cut(s)	534, 761, 794
Zsp2I	ATGCAT	1 cut(s)	583

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.