MD12G1162500.v1.1
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0017469
Physical Location & Seq
Reverse (-)
24409672 .. 24410559
888 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1162500.v1.1.491

Sequence Viewer

Length: 339 bp
ATGCCCTGTAAGTCATTAGGCATGCAGTTTCCATTTTTCAAGCTATTTGGAGTTCAACCAGTGCTATCTACATTAAAAGTGTTTGGGACATTTGTTTATCCCTATCTTAGACCATATAATTCATCAAAGGTAGATGCTAAGACAGATCAATGTGCATTCTTGGGCTACTCACTTGGATACAAATGGGTAATTTGTTATCAAAGAGTCAAGAAAAGATTGTTGATATCCAGACATGTGATTCTAGTTCACATCCAATCCTTGTTCCTTATGTTCTTCCTTGTCTCAGTAATGACAATAGTACATCTCACCCTAGAACAAGATCTCAGTCAGTGCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

113

Amino Acids

13.02

Weight (kDa)

9.58

Isoelectric Point (pI)

35.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_retrovirus PF25597 32 - 81 9.5e-11 Retroviral polymerase SH3-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017469)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g22952
malus_domestica MD03G1249400.v1.1 MD12G1162500.v1.1
prunus_persica Prupe.3G159700_v2.0.a1 Prupe.8G004800_v2.0.a1
pyrus_communis pycom01g01870 pycom01g13710 pycom15g18800 pycom420g01040
rosa_roxburghii Rroxscaffold_4G00320150 Rroxscaffold_7G00185690
rosa_wichuraiana Rw5G007100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 300
AflIII ACRYGT 1 cut(s) 232
AgsI TTSAA 2 cut(s) 40, 56
AluBI AGCT 1 cut(s) 43
AluI AGCT 1 cut(s) 43
Alw26I GTCTC 1 cut(s) 286
AsuHPI GGTGA 1 cut(s) 298
BciVI GTATCC 1 cut(s) 170
BcoDI GTCTC 1 cut(s) 286
BfaI CTAG 2 cut(s) 242, 311
BfuI GTATCC 1 cut(s) 170
BglII AGATCT 1 cut(s) 319
BmsI GCATC 1 cut(s) 124
Bse1I ACTGG 1 cut(s) 59
BseGI GGATG 1 cut(s) 249
BseMII CTCAG 2 cut(s) 297, 337
BseNI ACTGG 1 cut(s) 59
BslFI GGGAC 1 cut(s) 100
BsmAI GTCTC 1 cut(s) 286
BsmFI GGGAC 1 cut(s) 100
BsmI GAATGC 1 cut(s) 155
Bsp143I GATC 2 cut(s) 145, 319
BspCNI CTCAG 2 cut(s) 296, 336
BsrI ACTGG 1 cut(s) 59
BssMI GATC 2 cut(s) 145, 319
BstC8I GCNNGC 1 cut(s) 23
BstDEI CTNAG 4 cut(s) 107, 138, 283, 323
BstF5I GGATG 1 cut(s) 249
BstKTI GATC 2 cut(s) 148, 322
BstMAI GTCTC 1 cut(s) 286
BstMBI GATC 2 cut(s) 145, 319
BstNSI RCATGY 2 cut(s) 25, 236
BstX2I RGATCY 1 cut(s) 319
BstYI RGATCY 1 cut(s) 319
BsuI GTATCC 1 cut(s) 170
BtsCI GGATG 1 cut(s) 249
BtsIMutI CAGTG 2 cut(s) 66, 335
Cac8I GCNNGC 1 cut(s) 23
Csp6I GTAC 1 cut(s) 299
CviAII CATG 2 cut(s) 22, 233
CviJI RGCY 2 cut(s) 43, 165
CviKI_1 RGCY 2 cut(s) 43, 165
CviQI GTAC 1 cut(s) 299
DdeI CTNAG 4 cut(s) 107, 138, 283, 323
DpnI GATC 2 cut(s) 147, 321
DpnII GATC 2 cut(s) 145, 319
Eco32I GATATC 1 cut(s) 225
EcoRV GATATC 1 cut(s) 225
FaeI CATG 2 cut(s) 25, 236
FaiI YATR 7 cut(s) 23, 115, 117, 234, 269, 335, 337
FaqI GGGAC 1 cut(s) 100
FatI CATG 2 cut(s) 21, 232
FokI GGATG 1 cut(s) 236
FspBI CTAG 2 cut(s) 242, 311
Hin1II CATG 2 cut(s) 25, 236
HinfI GANTC 2 cut(s) 204, 238
HphI GGTGA 1 cut(s) 298
Hpy166II GTNNAC 1 cut(s) 247
Hpy188III TCNNGA 2 cut(s) 208, 228
Hpy8I GTNNAC 1 cut(s) 247
HpyCH4V TGCA 3 cut(s) 25, 155, 333
HpyF3I CTNAG 4 cut(s) 107, 138, 283, 323
Hsp92II CATG 2 cut(s) 25, 236
Kzo9I GATC 2 cut(s) 145, 319
LpnPI CCDG 3 cut(s) 19, 72, 241
LweI GCATC 1 cut(s) 124
MaeI CTAG 2 cut(s) 242, 311
MalI GATC 2 cut(s) 147, 321
MboI GATC 2 cut(s) 145, 319
MboII GAAGA 1 cut(s) 265
MflI RGATCY 1 cut(s) 319
MluCI AATT 2 cut(s) 118, 189
MlyI GAGTC 1 cut(s) 213
MseI TTAA 1 cut(s) 74
Mva1269I GAATGC 1 cut(s) 155
NdeII GATC 2 cut(s) 145, 319
NlaIII CATG 2 cut(s) 25, 236
NspI RCATGY 2 cut(s) 25, 236
PaeI GCATGC 1 cut(s) 25
PciI ACATGT 1 cut(s) 232
PctI GAATGC 1 cut(s) 155
PfeI GAWTC 1 cut(s) 238
PleI GAGTC 1 cut(s) 212
PpsI GAGTC 1 cut(s) 212
PscI ACATGT 1 cut(s) 232
PsuI RGATCY 1 cut(s) 319
RsaI GTAC 1 cut(s) 300
RsaNI GTAC 1 cut(s) 299
SaqAI TTAA 1 cut(s) 74
Sau3AI GATC 2 cut(s) 145, 319
SchI GAGTC 1 cut(s) 213
SetI ASST 2 cut(s) 45, 132
SfaNI GCATC 1 cut(s) 124
SphI GCATGC 1 cut(s) 25
Sse9I AATT 2 cut(s) 118, 189
SspMI CTAG 2 cut(s) 242, 311
TasI AATT 2 cut(s) 118, 189
TatI WGTACW 1 cut(s) 298
TfiI GAWTC 1 cut(s) 238
Tru1I TTAA 1 cut(s) 74
Tru9I TTAA 1 cut(s) 74
TscAI CASTG 2 cut(s) 66, 335
TspDTI ATGAA 1 cut(s) 111
TspRI CASTG 2 cut(s) 66, 335
XceI RCATGY 2 cut(s) 25, 236
XspI CTAG 2 cut(s) 242, 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.