MD12G1165000.v1.1

Belongs to the yippee family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007629
Physical Location & Seq
Reverse (-)
24632854 .. 24634915
2062 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1165000.v1.1.491

Sequence Viewer

Length: 390 bp
ATGGGAAGGCTGTTTGTGATCACTCTTGAGGAAAACATGTATAGCTGCAAGCACTGCAGAACCCATCTCGCTCTTGTCGACGACGTAATCTCTAAGTCTTTTCACTCCAGGCATGGGAAGGCTTATCTTTTCGATAAAGTCGTAAATGTCACACTTGGAGAGAAAGAAGAGCGGATCATGATAACAGGATTGCATACCGTTGCTGACATATTTTGTGTTTCGTGTGGATCAAATGTGGGATGGAAATATGAGGCGGCGCATGAGAAATCCCAGAAGTACAAGGAAGGAAAGTGTATCCTTGAGAGATTCCAGATTCTCGGTCCTGATGGAAGCAACTACTCAATCGCTCTCGAAGCTCAGATTGGCGGAAGCGATGGTGATGAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Yippee-Mis18

Protein Analysis

130

Amino Acids

14.39

Weight (kDa)

6.05

Isoelectric Point (pI)

21.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yippee-Mis18 PF03226 14 - 105 8.8e-14 Yippee zinc-binding/DNA-binding /Mis18, centromere assembly
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 172
AccI GTMKAC 1 cut(s) 78
AciI CCGC 3 cut(s) 172, 254, 366
AclWI GGATC 2 cut(s) 182, 235
AfaI GTAC 1 cut(s) 278
AfiI CCNNNNNNNGG 1 cut(s) 114
AflIII ACRYGT 1 cut(s) 36
AjnI CCWGG 1 cut(s) 107
AjuI GAANNNNNNNTTGG 2 cut(s) 345, 377
AluBI AGCT 2 cut(s) 45, 356
AluI AGCT 2 cut(s) 45, 356
AlwI GGATC 2 cut(s) 182, 235
ApeKI GCWGC 1 cut(s) 45
AspLEI GCGC 1 cut(s) 259
AspS9I GGNCC 1 cut(s) 320
AsuHPI GGTGA 1 cut(s) 389
AvaII GGWCC 1 cut(s) 320
BbvI GCAGC 1 cut(s) 32
BccI CCATC 4 cut(s) 72, 234, 320, 368
BciT130I CCWGG 1 cut(s) 109
BciVI GTATCC 1 cut(s) 305
BclI TGATCA 1 cut(s) 18
BfmI CTRYAG 1 cut(s) 55
BfuI GTATCC 1 cut(s) 305
BisI GCNGC 2 cut(s) 46, 255
BlsI GCNGC 2 cut(s) 47, 256
Bme1390I CCNGG 1 cut(s) 109
Bme18I GGWCC 1 cut(s) 320
BmgT120I GGNCC 1 cut(s) 320
BmrFI CCNGG 1 cut(s) 109
BpmI CTGGAG 1 cut(s) 91
BpuEI CTTGAG 2 cut(s) 47, 320
Bsc4I CCNNNNNNNGG 1 cut(s) 114
BseBI CCWGG 1 cut(s) 109
BseGI GGATG 1 cut(s) 245
BseLI CCNNNNNNNGG 1 cut(s) 114
BseMII CTCAG 1 cut(s) 371
BseXI GCAGC 1 cut(s) 32
BslI CCNNNNNNNGG 1 cut(s) 114
Bsp143I GATC 3 cut(s) 18, 174, 227
BspACI CCGC 3 cut(s) 172, 254, 366
BspCNI CTCAG 1 cut(s) 370
BspHI TCATGA 1 cut(s) 177
BspMAI CTGCAG 1 cut(s) 59
BspPI GGATC 2 cut(s) 182, 235
BspQI GCTCTTC 1 cut(s) 162
BsrBI CCGCTC 1 cut(s) 172
BssMI GATC 3 cut(s) 18, 174, 227
Bst2UI CCWGG 1 cut(s) 109
Bst4CI ACNGT 1 cut(s) 199
Bst6I CTCTTC 1 cut(s) 162
BstAPI GCANNNNNTGC 1 cut(s) 54
BstC8I GCNNGC 1 cut(s) 50
BstDEI CTNAG 2 cut(s) 93, 357
BstF5I GGATG 1 cut(s) 245
BstHHI GCGC 1 cut(s) 259
BstKTI GATC 3 cut(s) 21, 177, 230
BstMBI GATC 3 cut(s) 18, 174, 227
BstMWI GCNNNNNNNGC 2 cut(s) 54, 353
BstNI CCWGG 1 cut(s) 109
BstNSI RCATGY 1 cut(s) 40
BstSCI CCNGG 1 cut(s) 107
BstSFI CTRYAG 1 cut(s) 55
BstV1I GCAGC 1 cut(s) 32
BsuI GTATCC 1 cut(s) 305
BtsCI GGATG 1 cut(s) 245
BtsI GCAGTG 1 cut(s) 52
BtsIMutI CAGTG 1 cut(s) 52
Cac8I GCNNGC 1 cut(s) 50
CciI TCATGA 1 cut(s) 177
CfoI GCGC 1 cut(s) 259
Cfr13I GGNCC 1 cut(s) 320
Csp6I GTAC 1 cut(s) 277
CviAII CATG 4 cut(s) 37, 113, 178, 260
CviJI RGCY 5 cut(s) 10, 45, 122, 356, 386
CviKI_1 RGCY 5 cut(s) 10, 45, 122, 356, 386
CviQI GTAC 1 cut(s) 277
DdeI CTNAG 2 cut(s) 93, 357
DpnI GATC 3 cut(s) 20, 176, 229
DpnII GATC 3 cut(s) 18, 174, 227
Eam1104I CTCTTC 1 cut(s) 162
EarI CTCTTC 1 cut(s) 162
EciI GGCGGA 1 cut(s) 381
Eco47I GGWCC 1 cut(s) 320
EcoRII CCWGG 1 cut(s) 107
FaeI CATG 4 cut(s) 40, 116, 181, 263
FaiI YATR 8 cut(s) 38, 42, 114, 179, 195, 209, 249, 261
FatI CATG 4 cut(s) 36, 112, 177, 259
FbaI TGATCA 1 cut(s) 18
FblI GTMKAC 1 cut(s) 78
Fnu4HI GCNGC 2 cut(s) 46, 255
FokI GGATG 1 cut(s) 252
Fsp4HI GCNGC 2 cut(s) 46, 255
GlaI GCGC 1 cut(s) 258
GluI GCNGC 2 cut(s) 46, 255
GsuI CTGGAG 1 cut(s) 91
HhaI GCGC 1 cut(s) 259
Hin1II CATG 4 cut(s) 40, 116, 181, 263
Hin6I GCGC 1 cut(s) 257
HinP1I GCGC 1 cut(s) 257
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HinfI GANTC 2 cut(s) 306, 313
HphI GGTGA 1 cut(s) 389
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 1 cut(s) 360
Hpy188III TCNNGA 5 cut(s) 26, 178, 310, 323, 350
Hpy8I GTNNAC 1 cut(s) 79
Hpy99I CGWCG 2 cut(s) 83, 86
HpyAV CCTTC 2 cut(s) 112, 278
HpyCH4III ACNGT 1 cut(s) 199
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 3 cut(s) 48, 57, 193
HpyF10VI GCNNNNNNNGC 2 cut(s) 54, 353
HpyF3I CTNAG 2 cut(s) 93, 357
HpySE526I ACGT 1 cut(s) 84
Hsp92II CATG 4 cut(s) 40, 116, 181, 263
HspAI GCGC 1 cut(s) 257
Ksp22I TGATCA 1 cut(s) 18
Kzo9I GATC 3 cut(s) 18, 174, 227
LguI GCTCTTC 1 cut(s) 162
LpnPI CCDG 6 cut(s) 94, 121, 171, 284, 323, 336
Lsp1109I GCAGC 1 cut(s) 32
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 1 cut(s) 148
MalI GATC 3 cut(s) 20, 176, 229
MbiI CCGCTC 1 cut(s) 172
MboI GATC 3 cut(s) 18, 174, 227
MboII GAAGA 1 cut(s) 179
MnlI CCTC 3 cut(s) 22, 244, 376
MspR9I CCNGG 1 cut(s) 109
MvaI CCWGG 1 cut(s) 109
MwoI GCNNNNNNNGC 2 cut(s) 54, 353
NdeII GATC 3 cut(s) 18, 174, 227
NlaIII CATG 4 cut(s) 40, 116, 181, 263
NmuCI GTSAC 1 cut(s) 148
NspI RCATGY 1 cut(s) 40
PagI TCATGA 1 cut(s) 177
PciI ACATGT 1 cut(s) 36
PciSI GCTCTTC 1 cut(s) 162
PcsI WCGNNNNNNNCGW 2 cut(s) 75, 138
PfeI GAWTC 2 cut(s) 306, 313
PkrI GCNGC 2 cut(s) 47, 256
PscI ACATGT 1 cut(s) 36
Psp6I CCWGG 1 cut(s) 107
PspGI CCWGG 1 cut(s) 107
PspPI GGNCC 1 cut(s) 320
PstI CTGCAG 1 cut(s) 59
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
SalI GTCGAC 1 cut(s) 77
SapI GCTCTTC 1 cut(s) 162
SatI GCNGC 2 cut(s) 46, 255
Sau3AI GATC 3 cut(s) 18, 174, 227
Sau96I GGNCC 1 cut(s) 320
ScrFI CCNGG 1 cut(s) 109
SetI ASST 3 cut(s) 47, 87, 358
SfcI CTRYAG 1 cut(s) 55
SinI GGWCC 1 cut(s) 320
SmlI CTYRAG 2 cut(s) 26, 299
SmoI CTYRAG 2 cut(s) 26, 299
SsiI CCGC 3 cut(s) 172, 254, 366
StyD4I CCNGG 1 cut(s) 107
TaaI ACNGT 1 cut(s) 199
TaiI ACGT 1 cut(s) 87
TaqI TCGA 3 cut(s) 78, 132, 351
TaqII GACCGA 1 cut(s) 308
TatI WGTACW 1 cut(s) 276
TauI GCSGC 1 cut(s) 257
TfiI GAWTC 2 cut(s) 306, 313
TscAI CASTG 1 cut(s) 59
TseFI GTSAC 1 cut(s) 148
TseI GCWGC 1 cut(s) 45
Tsp45I GTSAC 1 cut(s) 148
TspRI CASTG 1 cut(s) 59
VpaK11BI GGWCC 1 cut(s) 320
XceI RCATGY 1 cut(s) 40
XmiI GTMKAC 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.