Rorug03G0063000

Belongs to the yippee family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0007629
Physical Location & Seq
Forward (+)
4687896 .. 4690052
2157 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0063000.1

Sequence Viewer

Length: 828 bp
ATGGATTGCAGGCCTTTCGAAGTAACGGTGTTCGGCGCCAATGACATCAAGGACGTCAACATGTTCTCCAAGATGGACGTCTACGCCGTCGTCTCCGTCTCCGGTAACGGCTTCAGGAAGCAGAAGCTCCGCACCCCCGTCCACAAAGACGCCGGCACCAACCCCAAATGGAACCACACCCTCAAGTTCACCCTCGACAACTCCGCCCTCGACCGCCTCAAAATCAAAATCAAGCTCATCTCTGACCGCACCCTCGGCGACACCAAGATCGGAAAGGCCGTGGTTCCGGTCAAGGACCTCCTGCAAAACGAGAAGAACGACAAGCACGTGACCTACCCGGTCCTGACTGCCAAGGGGAGACCCAAAGGCAGCGTCAGTTTCGGGTACAAGTTCGGCGAGAAATTCACGGCGCCTCTTCCGGTCCTGCATCAGGCCCACAAGAAGGCCCACGAGCCCGTTATGGCGTACCCGGCGGGCCATGCCGGGTCGAGCTCCGCTTACCCGCCACCCGGAGTGGCCCACCCGGTTTATCATCCTTACCCGCCTCAGCCACAACCGGGTTACGGGTACCCGCCGCAGCAGCAACCGGGTTACGGGTACCCGCCGCAGCAGCAGCCGGGTTACGGGTACCCGCCGGTAGTGCAGCCGGGTTATGGGTACGTGCAGCAGCCGCAGAAGCCCAAAAGAGGAAGAGGGAACATGGGGCTTGGGTTGGGAGCGGGACTGTTGGGTGGGTTGCTGATCGGAGATATGGTTTCCGATGTCGGTGAGATGGGGGCGTATGATGCTGGTTATGATGATGGTATGGGTGATGGGTTTGATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Yippee-Mis18

Protein Analysis

275

Amino Acids

29.84

Weight (kDa)

9.28

Isoelectric Point (pI)

34.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 7 - 108 3.1e-16 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 57, 81
Acc65I GGTACC 3 cut(s) 567, 597, 627
AccB1I GGYRCC 6 cut(s) 35, 155, 409, 567, 597, 627
AccBSI CCGCTC 1 cut(s) 719
AccI GTMKAC 1 cut(s) 81
AcsI RAATTY 1 cut(s) 401
AcuI CTGAAG 1 cut(s) 97
AcvI CACGTG 1 cut(s) 328
AcyI GRCGYC 5 cut(s) 36, 54, 78, 150, 410
AfaI GTAC 6 cut(s) 386, 467, 569, 599, 629, 659
AfiI CCNNNNNNNGG 9 cut(s) 430, 442, 509, 557, 563, 593, 623, 653, 686
AflIII ACRYGT 1 cut(s) 60
AluBI AGCT 3 cut(s) 127, 235, 492
AluI AGCT 3 cut(s) 127, 235, 492
Alw21I GWGCWC 1 cut(s) 494
Alw26I GTCTC 3 cut(s) 97, 103, 352
AoxI GGCC 6 cut(s) 11, 276, 432, 444, 475, 516
ApeKI GCWGC 9 cut(s) 369, 577, 580, 607, 610, 613, 643, 664, 667
ApoI RAATTY 1 cut(s) 401
ArsI GACNNNNNNTTYG 2 cut(s) 357, 389
Asp718I GGTACC 3 cut(s) 567, 597, 627
AspLEI GCGC 2 cut(s) 38, 412
AspS9I GGNCC 7 cut(s) 295, 340, 421, 433, 445, 475, 517
AsuC2I CCSGG 9 cut(s) 338, 470, 484, 510, 524, 558, 588, 618, 648
AsuHPI GGTGA 3 cut(s) 181, 779, 821
AsuII TTCGAA 1 cut(s) 18
AvaII GGWCC 3 cut(s) 295, 340, 421
BanI GGYRCC 6 cut(s) 35, 155, 409, 567, 597, 627
BanII GRGCYC 2 cut(s) 456, 494
BauI CACGAG 1 cut(s) 449
BbrPI CACGTG 1 cut(s) 328
Bbv12I GWGCWC 1 cut(s) 494
BbvCI CCTCAGC 1 cut(s) 546
BbvI GCAGC 9 cut(s) 381, 589, 592, 619, 622, 625, 655, 676, 679
BccI CCATC 4 cut(s) 67, 766, 794, 806
BceAI ACGGC 4 cut(s) 71, 124, 263, 423
BcgI CGANNNNNNTGC 1 cut(s) 32
BcnI CCSGG 9 cut(s) 338, 470, 484, 510, 524, 558, 588, 618, 648
BcoDI GTCTC 3 cut(s) 97, 103, 352
BfaI CTAG 1 cut(s) 826
BfoI RGCGCY 2 cut(s) 39, 413
Bme1390I CCNGG 9 cut(s) 338, 470, 484, 510, 524, 558, 588, 618, 648
Bme18I GGWCC 3 cut(s) 295, 340, 421
BmgT120I GGNCC 7 cut(s) 295, 340, 421, 433, 445, 475, 517
BmiI GGNNCC 8 cut(s) 37, 157, 173, 285, 411, 569, 599, 629
BmrFI CCNGG 9 cut(s) 338, 470, 484, 510, 524, 558, 588, 618, 648
BmsI GCATC 2 cut(s) 436, 775
Bpu10I CCTNAGC 1 cut(s) 546
Bpu14I TTCGAA 1 cut(s) 18
BpuEI CTTGAG 1 cut(s) 167
BpuMI CCSGG 9 cut(s) 338, 470, 484, 510, 524, 558, 588, 618, 648
BsaAI YACGTR 2 cut(s) 328, 661
BsaHI GRCGYC 5 cut(s) 36, 54, 78, 150, 410
BsaI GGTCTC 1 cut(s) 352
BsaJI CCNNGG 3 cut(s) 253, 279, 351
BsaWI WCCGGW 3 cut(s) 101, 286, 418
BsaXI ACNNNNNCTCC 4 cut(s) 50, 80, 738, 768
Bsc4I CCNNNNNNNGG 9 cut(s) 430, 442, 509, 557, 563, 593, 623, 653, 686
Bse118I RCCGGY 2 cut(s) 152, 634
BseDI CCNNGG 3 cut(s) 253, 279, 351
BseGI GGATG 1 cut(s) 532
BseLI CCNNNNNNNGG 9 cut(s) 430, 442, 509, 557, 563, 593, 623, 653, 686
BseMII CTCAG 1 cut(s) 560
BseXI GCAGC 9 cut(s) 381, 589, 592, 619, 622, 625, 655, 676, 679
BsgI GTGCAG 2 cut(s) 662, 683
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 6 cut(s) 13, 278, 434, 446, 477, 518
BshNI GGYRCC 6 cut(s) 35, 155, 409, 567, 597, 627
BsiEI CGRYCG 1 cut(s) 214
BsiHKAI GWGCWC 1 cut(s) 494
BslFI GGGAC 1 cut(s) 735
BslI CCNNNNNNNGG 9 cut(s) 430, 442, 509, 557, 563, 593, 623, 653, 686
BsmAI GTCTC 3 cut(s) 97, 103, 352
BsmBI CGTCTC 2 cut(s) 97, 103
BsmFI GGGAC 1 cut(s) 735
BsnI GGCC 6 cut(s) 13, 278, 434, 446, 477, 518
Bso31I GGTCTC 1 cut(s) 352
Bsp119I TTCGAA 1 cut(s) 18
Bsp1286I GDGCHC 2 cut(s) 456, 494
Bsp143I GATC 2 cut(s) 267, 741
BspANI GGCC 6 cut(s) 13, 278, 434, 446, 477, 518
BspCNI CTCAG 1 cut(s) 559
BspLI GGNNCC 8 cut(s) 37, 157, 173, 285, 411, 569, 599, 629
BspT104I TTCGAA 1 cut(s) 18
BspT107I GGYRCC 6 cut(s) 35, 155, 409, 567, 597, 627
BspTNI GGTCTC 1 cut(s) 352
BsrBI CCGCTC 1 cut(s) 719
BsrFI RCCGGY 2 cut(s) 152, 634
BssAI RCCGGY 2 cut(s) 152, 634
BssECI CCNNGG 3 cut(s) 253, 279, 351
BssMI GATC 2 cut(s) 267, 741
BssNI GRCGYC 5 cut(s) 36, 54, 78, 150, 410
BssSI CACGAG 1 cut(s) 449
BssT1I CCWWGG 1 cut(s) 351
Bst2BI CACGAG 1 cut(s) 449
Bst4CI ACNGT 2 cut(s) 28, 726
Bst6I CTCTTC 2 cut(s) 420, 685
BstACI GRCGYC 5 cut(s) 36, 54, 78, 150, 410
BstBAI YACGTR 2 cut(s) 328, 661
BstBI TTCGAA 1 cut(s) 18
BstC8I GCNNGC 3 cut(s) 11, 154, 475
BstDEI CTNAG 1 cut(s) 546
BstDSI CCRYGG 1 cut(s) 279
BstENI CCTNNNNNAGG 1 cut(s) 428
BstF5I GGATG 1 cut(s) 532
BstH2I RGCGCY 2 cut(s) 39, 413
BstHHI GCGC 2 cut(s) 38, 412
BstKTI GATC 2 cut(s) 270, 744
BstMAI GTCTC 3 cut(s) 97, 103, 352
BstMBI GATC 2 cut(s) 267, 741
BstMCI CGRYCG 1 cut(s) 214
BstNSI RCATGY 1 cut(s) 64
BstSCI CCNGG 9 cut(s) 336, 468, 482, 508, 522, 556, 586, 616, 646
BstV1I GCAGC 9 cut(s) 381, 589, 592, 619, 622, 625, 655, 676, 679
BsuRI GGCC 6 cut(s) 13, 278, 434, 446, 477, 518
BtgI CCRYGG 1 cut(s) 279
BtsCI GGATG 1 cut(s) 532
Cac8I GCNNGC 3 cut(s) 11, 154, 475
CfoI GCGC 2 cut(s) 38, 412
Cfr10I RCCGGY 2 cut(s) 152, 634
Cfr13I GGNCC 7 cut(s) 295, 340, 421, 433, 445, 475, 517
CseI GACGC 2 cut(s) 158, 361
Csp6I GTAC 6 cut(s) 385, 466, 568, 598, 628, 658
CviAII CATG 3 cut(s) 61, 479, 700
CviQI GTAC 6 cut(s) 385, 466, 568, 598, 628, 658
DdeI CTNAG 1 cut(s) 546
DinI GGCGCC 2 cut(s) 37, 411
DpnI GATC 2 cut(s) 269, 743
DpnII GATC 2 cut(s) 267, 741
Eam1104I CTCTTC 2 cut(s) 420, 685
EarI CTCTTC 2 cut(s) 420, 685
EciI GGCGGA 1 cut(s) 193
Ecl136II GAGCTC 1 cut(s) 492
Eco130I CCWWGG 1 cut(s) 351
Eco147I AGGCCT 1 cut(s) 13
Eco24I GRGCYC 2 cut(s) 456, 494
Eco31I GGTCTC 1 cut(s) 352
Eco47I GGWCC 3 cut(s) 295, 340, 421
Eco53kI GAGCTC 1 cut(s) 492
Eco57I CTGAAG 1 cut(s) 97
Eco72I CACGTG 1 cut(s) 328
EcoICRI GAGCTC 1 cut(s) 492
EcoNI CCTNNNNNAGG 1 cut(s) 428
EcoO109I RGGNCCY 1 cut(s) 295
EcoT14I CCWWGG 1 cut(s) 351
EcoT38I GRGCYC 2 cut(s) 456, 494
EgeI GGCGCC 2 cut(s) 37, 411
EheI GGCGCC 2 cut(s) 37, 411
ErhI CCWWGG 1 cut(s) 351
Esp3I CGTCTC 2 cut(s) 97, 103
FaeI CATG 3 cut(s) 64, 482, 703
FaiI YATR 9 cut(s) 62, 461, 480, 654, 701, 752, 783, 795, 806
FaqI GGGAC 1 cut(s) 735
FatI CATG 3 cut(s) 60, 478, 699
FauI CCCGC 7 cut(s) 466, 510, 549, 579, 609, 639, 712
FblI GTMKAC 1 cut(s) 81
FokI GGATG 1 cut(s) 519
FriOI GRGCYC 2 cut(s) 456, 494
FspBI CTAG 1 cut(s) 826
GlaI GCGC 2 cut(s) 37, 411
HaeII RGCGCY 2 cut(s) 39, 413
HaeIII GGCC 6 cut(s) 13, 278, 434, 446, 477, 518
HgaI GACGC 2 cut(s) 158, 361
HhaI GCGC 2 cut(s) 38, 412
Hin1I GRCGYC 5 cut(s) 36, 54, 78, 150, 410
Hin1II CATG 3 cut(s) 64, 482, 703
Hin6I GCGC 2 cut(s) 36, 410
HinP1I GCGC 2 cut(s) 36, 410
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HphI GGTGA 3 cut(s) 181, 779, 821
Hpy166II GTNNAC 4 cut(s) 58, 82, 142, 189
Hpy188I TCNGA 4 cut(s) 244, 272, 746, 760
Hpy188III TCNNGA 2 cut(s) 115, 343
Hpy8I GTNNAC 4 cut(s) 58, 82, 142, 189
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 1 cut(s) 436
HpyCH4III ACNGT 2 cut(s) 28, 726
HpyCH4IV ACGT 4 cut(s) 54, 78, 327, 660
HpyCH4V TGCA 5 cut(s) 9, 304, 427, 643, 664
HpyF3I CTNAG 1 cut(s) 546
HpySE526I ACGT 4 cut(s) 54, 78, 327, 660
Hsp92I GRCGYC 5 cut(s) 36, 54, 78, 150, 410
Hsp92II CATG 3 cut(s) 64, 482, 703
HspAI GCGC 2 cut(s) 36, 410
KasI GGCGCC 2 cut(s) 35, 409
KpnI GGTACC 3 cut(s) 571, 601, 631
KroI GCCGGC 1 cut(s) 152
KroNI GCCGGC 1 cut(s) 154
Kzo9I GATC 2 cut(s) 267, 741
LmnI GCTCC 3 cut(s) 132, 497, 716
Lsp1109I GCAGC 9 cut(s) 381, 589, 592, 619, 622, 625, 655, 676, 679
LweI GCATC 2 cut(s) 436, 775
MaeI CTAG 1 cut(s) 826
MaeII ACGT 4 cut(s) 54, 78, 327, 660
MaeIII GTNAC 6 cut(s) 22, 104, 328, 560, 590, 620
MalI GATC 2 cut(s) 269, 743
MbiI CCGCTC 1 cut(s) 719
MboI GATC 2 cut(s) 267, 741
MboII GAAGA 3 cut(s) 325, 407, 702
MhlI GDGCHC 2 cut(s) 456, 494
MluCI AATT 1 cut(s) 401
Mly113I GGCGCC 2 cut(s) 36, 410
MroNI GCCGGC 1 cut(s) 152
MspR9I CCNGG 9 cut(s) 338, 470, 484, 510, 524, 558, 588, 618, 648
NaeI GCCGGC 1 cut(s) 154
NarI GGCGCC 2 cut(s) 36, 410
NciI CCSGG 9 cut(s) 338, 470, 484, 510, 524, 558, 588, 618, 648
NdeII GATC 2 cut(s) 267, 741
NgoMIV GCCGGC 1 cut(s) 152
NlaIII CATG 3 cut(s) 64, 482, 703
NlaIV GGNNCC 8 cut(s) 37, 157, 173, 285, 411, 569, 599, 629
NmeAIII GCCGAG 1 cut(s) 234
NmuCI GTSAC 1 cut(s) 328
NspI RCATGY 1 cut(s) 64
NspV TTCGAA 1 cut(s) 18
PceI AGGCCT 1 cut(s) 13
PciI ACATGT 1 cut(s) 60
PcsI WCGNNNNNNNCGW 4 cut(s) 84, 276, 315, 324
PdiI GCCGGC 1 cut(s) 154
PluTI GGCGCC 2 cut(s) 39, 413
PmaCI CACGTG 1 cut(s) 328
PmlI CACGTG 1 cut(s) 328
Ppu21I YACGTR 2 cut(s) 328, 661
PpuMI RGGWCCY 1 cut(s) 295
PscI ACATGT 1 cut(s) 60
Psp124BI GAGCTC 1 cut(s) 494
Psp5II RGGWCCY 1 cut(s) 295
PspCI CACGTG 1 cut(s) 328
PspN4I GGNNCC 8 cut(s) 37, 157, 173, 285, 411, 569, 599, 629
PspPI GGNCC 7 cut(s) 295, 340, 421, 433, 445, 475, 517
PspPPI RGGWCCY 1 cut(s) 295
RsaI GTAC 6 cut(s) 386, 467, 569, 599, 629, 659
RsaNI GTAC 6 cut(s) 385, 466, 568, 598, 628, 658
SacI GAGCTC 1 cut(s) 494
Sau3AI GATC 2 cut(s) 267, 741
Sau96I GGNCC 7 cut(s) 295, 340, 421, 433, 445, 475, 517
ScrFI CCNGG 9 cut(s) 338, 470, 484, 510, 524, 558, 588, 618, 648
SduI GDGCHC 2 cut(s) 456, 494
SetI ASST 9 cut(s) 57, 81, 129, 237, 300, 330, 335, 494, 663
SfaNI GCATC 2 cut(s) 436, 775
SfoI GGCGCC 2 cut(s) 37, 411
SfuI TTCGAA 1 cut(s) 18
SinI GGWCC 3 cut(s) 295, 340, 421
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
Sse9I AATT 1 cut(s) 401
SseBI AGGCCT 1 cut(s) 13
SspDI GGCGCC 2 cut(s) 35, 409
SspMI CTAG 1 cut(s) 826
SstI GAGCTC 1 cut(s) 494
StuI AGGCCT 1 cut(s) 13
StyD4I CCNGG 9 cut(s) 336, 468, 482, 508, 522, 556, 586, 616, 646
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 2 cut(s) 28, 726
TaiI ACGT 4 cut(s) 57, 81, 330, 663
TaqI TCGA 4 cut(s) 18, 195, 210, 488
TasI AATT 1 cut(s) 401
TauI GCSGC 3 cut(s) 577, 607, 673
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 9 cut(s) 369, 577, 580, 607, 610, 613, 643, 664, 667
Tsp45I GTSAC 1 cut(s) 328
TspGWI ACGGA 1 cut(s) 85
VpaK11BI GGWCC 3 cut(s) 295, 340, 421
XagI CCTNNNNNAGG 1 cut(s) 428
XapI RAATTY 1 cut(s) 401
XceI RCATGY 1 cut(s) 64
XmiI GTMKAC 1 cut(s) 81
XspI CTAG 1 cut(s) 826
ZraI GACGTC 2 cut(s) 55, 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.