Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0010035

Physical Location & Seq

Genomic Coordinates Forward (+)

25838275 .. 25841565

Gene Structure 3291 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1177900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1560 bp

ATGGCCTCCATCAACTTCAACCCCTTCGAAAGCTGGTTCAAAAAACCCCCAAACCCCCTGCCACCCATCAACCTCCTCCCCCTCACTGACTCTTTCTTCCACCGCACCACCGCGGCCCAATCCCCTCCCAATTTCTCCTCCCTTAGCCTCTCAAACCTCTTCAACGGCCCCCCGAAACCCGACCCCAAAAACCCATCTGACCCCGAGCCGGAAAAACCCGGACACTACACCCAGATGCTGGAGCGGTTCTTCTGGGAGTGCGAGAACGACCCGGATTACAGACACTCCCCGGAAGTCGAGAAGATTCTGAACGAAGACCCGATTTTCGAAAAGAAGGAGAACCCAACGGAGGAGGAACTTCGGGAGAACGAGAGGTTCTGGAAGGAGTTCCGGGAGAGCCCGGTGGTGAAGTTTCTGGCCCAGGCGGAGAAGATTGCGGATATGGTGAATGAGCTGGAGCTGAAGGAGAATGATAGGCCGTACAGGGACGAGGATAAGAAGCTGTGGCAGGCGGTGCCACATGTTATTGGGCCGGACGGGAGGCCTATGCCGAGGAAGGCGATAAAGACGAGGCAGGAGTCTGATGATAAGTTCTGGGATTTTGCAAGGCAGTTCTTTTTTGGGCTTTGGGGGTTCCGCCAGCGCCCCTACCCACCCGGCCGGCCCATCGATGTTGCTCAGGCAATCGGGTATAAGCGGCTCGAAAAGCGGTATTATGATTTTATCATGAGGAGCGGTGGATTTTACTATAAGGACCGGTTAGGTCGAACAAGAGGTCCGTTGGAGCTAATAACCCTTAAAACGGCTTGGGGTGCTGGGATTATTGATAAAGACACGTTCATTTGGGGTGAGGACATGGATGAATGGGCTCCGATTCACATGGTTTACGGGTTGGAACCTGCTATTGCCACTTGGGAAGTTAGACTTGCGGCTGGTGCGACTGCTTTGATTCACAAACTACAAAAAGGAATTCCTCCTTGGGTTCCACTCAAGGGACGCGAGAAGAAAACCTATAAGCAGCTACAAGAGGAGGCTGTAGAAAGCAAGAGACGCGACTTAGCAGTACTTGAAGCTAACGATGGTGTTTGGCCAGGTGTTCGAACTCCGAGTCATGCTCTATTTCTTTGGGCTAGTGGCTCTGAATTGACAACAATTCTAGAAGAGGATCACATGCCGAACAAATACATTTCAAAAGCTCTTAGGGTTGAATTGTCTAAAATTATCCCTGGGTTAAGGCCATGGGAGGTTCTAAGTGTGGAACAAGCAATGGATCAGATAACATATGAGGGAGAGTGGTTCCGTGAACCACTTGGCTCGTACACGACAGGTCCTCCATACATTCGAGCTTGGAATAATGATGTCAAGAGACTGTATAATATTTTCGACAACCTAAGCAGCCGAGTCTACCAGAAACTGGAGCGAACAATTCCTGGTTTTGATAAGATAATGGAGAAAGTTCAAGCTGATGCTGCTGCAAGGGATGCCAAGCGGAAGGCAAGAAGAGAGGCAGAGATGAAAGCTGAGCAGGAAAAAGCCTTGGGAATTCAAAGTGATGGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006605 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0008565 GO:0009507 GO:0009526 GO:0009528 GO:0009536 GO:0009706 GO:0009941 GO:0015031 GO:0015833 GO:0016020 GO:0017038 GO:0019866 GO:0031090 GO:0031967 GO:0031969 GO:0031975 GO:0033036 GO:0033365 GO:0034613 GO:0042170 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045036 GO:0045037 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0065002 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0072594 GO:0072596 GO:0072598

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF4339

Protein Analysis

520

Amino Acids

60.28

Weight (kDa)

6.19

Isoelectric Point (pI)

42.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GYF_2	PF14237	249 - 298	3.4e-10	GYF domain 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010035)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01590
fragaria_vesca	FvH4_6g10790
malus_domestica	MD04G1164300.v1.1 MD12G1177900.v1.1
prunus_persica	Prupe.6G283400_v2.0.a1
pyrus_communis	pycom12g16830
rosa_chinensis	RchiOBHm_Chr3g0461841
rosa_laevigata	RLG00000024887 RLG00000024893
rosa_multiflora	Rmu_sc0004308.1_g000016 Rmu_sc0004308.1_g000019 Rmu_sc0007777.1_g000004 Rmu_sc0007777.1_g000014 Rmu_sc0007777.1_g000021
rosa_roxburghii	Rroxscaffold_6G00417870
rosa_rugosa	Rorug03G0054100 Rorug03G0054300
rosa_samantha	Rh3AG112200 Rh3CG117600 Rh3DG356900
rosa_wichuraiana	Rw3G009510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	907
AccB1I	GGYRCC	1 cut(s)	514
AccB7I	CCANNNNNTGG	2 cut(s)	238, 1414
AccBSI	CCGCTC	2 cut(s)	244, 735
AccI	GTMKAC	1 cut(s)	1404
AccII	CGCG	3 cut(s)	113, 999, 1053
AclWI	GGATC	2 cut(s)	1173, 1278
AcoI	YGGCCR	2 cut(s)	658, 1088
AcsI	RAATTY	2 cut(s)	969, 1542
AcuI	CTGAAG	1 cut(s)	482
AfaI	GTAC	3 cut(s)	482, 1065, 1319
AfiI	CCNNNNNNNGG	6 cut(s)	208, 238, 349, 802, 992, 1414
AflIII	ACRYGT	2 cut(s)	520, 834
AgeI	ACCGGT	1 cut(s)	756
AgsI	TTSAA	8 cut(s)	19, 40, 163, 1070, 1191, 1208, 1460, 1547
AjnI	CCWGG	4 cut(s)	420, 1090, 1225, 1429
AjuI	GAANNNNNNNTTGG	2 cut(s)	961, 993
AloI	GAACNNNNNNTCC	2 cut(s)	760, 792
Alw26I	GTCTC	2 cut(s)	1042, 1360
AlwI	GGATC	2 cut(s)	1173, 1278
AlwNI	CAGNNNCTG	2 cut(s)	238, 1414
Ama87I	CYCGRG	1 cut(s)	203
ApeKI	GCWGC	4 cut(s)	1018, 1395, 1469, 1472
ApoI	RAATTY	2 cut(s)	969, 1542
ArsI	GACNNNNNNTTYG	6 cut(s)	308, 340, 760, 792, 1093, 1125
AsiGI	ACCGGT	1 cut(s)	756
Asp700I	GAANNNNTTC	1 cut(s)	386
AspLEI	GCGC	1 cut(s)	645
AspS9I	GGNCC	8 cut(s)	115, 167, 418, 530, 663, 754, 776, 1328
AsuC2I	CCSGG	6 cut(s)	219, 272, 290, 392, 401, 657
AsuHPI	GGTGA	3 cut(s)	418, 457, 860
AsuII	TTCGAA	3 cut(s)	27, 327, 1099
AvaI	CYCGRG	1 cut(s)	203
AvaII	GGWCC	3 cut(s)	754, 776, 1328
BalI	TGGCCA	1 cut(s)	1090
BanI	GGYRCC	1 cut(s)	514
BanII	GRGCYC	2 cut(s)	401, 871
BbsI	GAAGAC	1 cut(s)	321
BbvI	GCAGC	4 cut(s)	1030, 1407, 1456, 1459
BccI	CCATC	6 cut(s)	17, 74, 202, 674, 1073, 1547
BceAI	ACGGC	3 cut(s)	181, 463, 819
BciT130I	CCWGG	4 cut(s)	422, 1092, 1227, 1431
BcnI	CCSGG	6 cut(s)	219, 272, 290, 392, 401, 657
BcoDI	GTCTC	2 cut(s)	1042, 1360
BfaI	CTAG	2 cut(s)	1131, 1157
BfmI	CTRYAG	1 cut(s)	1035
BfoI	RGCGCY	1 cut(s)	646
BfuAI	ACCTGC	1 cut(s)	907
BisI	GCNGC	7 cut(s)	114, 698, 930, 1019, 1396, 1470, 1473
BlpI	GCTNAGC	1 cut(s)	1521
BlsI	GCNGC	7 cut(s)	115, 699, 931, 1020, 1397, 1471, 1474
BmcAI	AGTACT	1 cut(s)	1065
Bme18I	GGWCC	3 cut(s)	754, 776, 1328
BmeT110I	CYCGRG	1 cut(s)	203
BmgT120I	GGNCC	8 cut(s)	115, 167, 418, 530, 663, 754, 776, 1328
BmiI	GGNNCC	7 cut(s)	169, 516, 635, 870, 897, 984, 1298
BmsI	GCATC	3 cut(s)	225, 1456, 1471
BpiI	GAAGAC	1 cut(s)	321
BplI	GAGNNNNNCTC	2 cut(s)	1099, 1131
BpmI	CTGGAG	3 cut(s)	260, 476, 1436
Bpu10I	CCTNAGC	3 cut(s)	143, 678, 1391
Bpu1102I	GCTNAGC	1 cut(s)	1521
Bpu14I	TTCGAA	3 cut(s)	27, 327, 1099
BpuEI	CTTGAG	1 cut(s)	974
BpuMI	CCSGG	6 cut(s)	219, 272, 290, 392, 401, 657
Bsa29I	ATCGAT	1 cut(s)	669
BsaJI	CCNNGG	9 cut(s)	111, 288, 420, 551, 977, 1225, 1226, 1238, 1536
BsaWI	WCCGGW	1 cut(s)	756
BsaXI	ACNNNNNCTCC	4 cut(s)	269, 299, 1315, 1345
Bsc4I	CCNNNNNNNGG	6 cut(s)	208, 238, 349, 802, 992, 1414
Bse118I	RCCGGY	2 cut(s)	660, 756
Bse1I	ACTGG	1 cut(s)	1419
Bse3DI	GCAATG	1 cut(s)	1272
BseBI	CCWGG	4 cut(s)	422, 1092, 1227, 1431
BseCI	ATCGAT	1 cut(s)	669
BseDI	CCNNGG	9 cut(s)	111, 288, 420, 551, 977, 1225, 1226, 1238, 1536
BseGI	GGATG	2 cut(s)	865, 1486
BseLI	CCNNNNNNNGG	6 cut(s)	208, 238, 349, 802, 992, 1414
BseMI	GCAATG	1 cut(s)	1272
BseMII	CTCAG	2 cut(s)	692, 1512
BseNI	ACTGG	1 cut(s)	1419
BseRI	GAGGAG	5 cut(s)	65, 127, 365, 745, 1043
BseX3I	CGGCCG	1 cut(s)	658
BseXI	GCAGC	4 cut(s)	1030, 1407, 1456, 1459
BseYI	CCCAGC	1 cut(s)	815
Bsh1236I	CGCG	3 cut(s)	113, 999, 1053
Bsh1285I	CGRYCG	1 cut(s)	661
BshNI	GGYRCC	1 cut(s)	514
BshTI	ACCGGT	1 cut(s)	756
BshVI	ATCGAT	1 cut(s)	669
BsiEI	CGRYCG	1 cut(s)	661
BsiHKCI	CYCGRG	1 cut(s)	203
BslFI	GGGAC	2 cut(s)	500, 1008
BslI	CCNNNNNNNGG	6 cut(s)	208, 238, 349, 802, 992, 1414
BsmAI	GTCTC	2 cut(s)	1042, 1360
BsmBI	CGTCTC	1 cut(s)	1042
BsmFI	GGGAC	2 cut(s)	500, 1008
BsoBI	CYCGRG	1 cut(s)	203
Bsp119I	TTCGAA	3 cut(s)	27, 327, 1099
Bsp1286I	GDGCHC	2 cut(s)	401, 871
Bsp143I	GATC	2 cut(s)	1165, 1270
Bsp1720I	GCTNAGC	1 cut(s)	1521
Bsp19I	CCATGG	1 cut(s)	1238
BspCNI	CTCAG	2 cut(s)	691, 1513
BspDI	ATCGAT	1 cut(s)	669
BspFNI	CGCG	3 cut(s)	113, 999, 1053
BspHI	TCATGA	1 cut(s)	726
BspLI	GGNNCC	7 cut(s)	169, 516, 635, 870, 897, 984, 1298
BspMI	ACCTGC	1 cut(s)	907
BspPI	GGATC	2 cut(s)	1173, 1278
BspT104I	TTCGAA	3 cut(s)	27, 327, 1099
BspT107I	GGYRCC	1 cut(s)	514
BsrBI	CCGCTC	2 cut(s)	244, 735
BsrDI	GCAATG	1 cut(s)	1272
BsrFI	RCCGGY	2 cut(s)	660, 756
BsrI	ACTGG	1 cut(s)	1419
BssAI	RCCGGY	2 cut(s)	660, 756
BssECI	CCNNGG	9 cut(s)	111, 288, 420, 551, 977, 1225, 1226, 1238, 1536
BssMI	GATC	2 cut(s)	1165, 1270
BssT1I	CCWWGG	3 cut(s)	977, 1238, 1536
Bst2UI	CCWGG	4 cut(s)	422, 1092, 1227, 1431
Bst4CI	ACNGT	1 cut(s)	1371
Bst6I	CTCTTC	3 cut(s)	164, 1155, 1495
BstAPI	GCANNNNNTGC	2 cut(s)	514, 1481
BstBI	TTCGAA	3 cut(s)	27, 327, 1099
BstC8I	GCNNGC	3 cut(s)	510, 641, 662
BstDEI	CTNAG	7 cut(s)	143, 678, 1057, 1199, 1250, 1391, 1521
BstDSI	CCRYGG	2 cut(s)	111, 1238
BstF5I	GGATG	2 cut(s)	865, 1486
BstFNI	CGCG	3 cut(s)	113, 999, 1053
BstH2I	RGCGCY	1 cut(s)	646
BstHHI	GCGC	1 cut(s)	645
BstKTI	GATC	2 cut(s)	1168, 1273
BstMAI	GTCTC	2 cut(s)	1042, 1360
BstMBI	GATC	2 cut(s)	1165, 1270
BstMCI	CGRYCG	1 cut(s)	661
BstMWI	GCNNNNNNNGC	7 cut(s)	514, 706, 812, 935, 1050, 1469, 1481
BstNI	CCWGG	4 cut(s)	422, 1092, 1227, 1431
BstNSI	RCATGY	2 cut(s)	524, 1174
BstSFI	CTRYAG	1 cut(s)	1035
BstUI	CGCG	3 cut(s)	113, 999, 1053
BstV1I	GCAGC	4 cut(s)	1030, 1407, 1456, 1459
BstV2I	GAAGAC	1 cut(s)	321
BstZI	CGGCCG	1 cut(s)	658
Bsu15I	ATCGAT	1 cut(s)	669
BsuTUI	ATCGAT	1 cut(s)	669
BtgI	CCRYGG	2 cut(s)	111, 1238
BtsCI	GGATG	2 cut(s)	865, 1486
BtsIMutI	CAGTG	1 cut(s)	84
BveI	ACCTGC	1 cut(s)	907
Cac8I	GCNNGC	3 cut(s)	510, 641, 662
CaiI	CAGNNNCTG	2 cut(s)	238, 1414
CciI	TCATGA	1 cut(s)	726
CfoI	GCGC	1 cut(s)	645
Cfr10I	RCCGGY	2 cut(s)	660, 756
Cfr13I	GGNCC	8 cut(s)	115, 167, 418, 530, 663, 754, 776, 1328
Cfr42I	CCGCGG	1 cut(s)	114
ClaI	ATCGAT	1 cut(s)	669
CseI	GACGC	2 cut(s)	1005, 1059
Csp6I	GTAC	3 cut(s)	481, 1064, 1318
CspAI	ACCGGT	1 cut(s)	756
CviAII	CATG	7 cut(s)	521, 727, 856, 880, 1112, 1171, 1239
CviQI	GTAC	3 cut(s)	481, 1064, 1318
DdeI	CTNAG	7 cut(s)	143, 678, 1057, 1199, 1250, 1391, 1521
DpnI	GATC	2 cut(s)	1167, 1272
DpnII	GATC	2 cut(s)	1165, 1270
EaeI	YGGCCR	2 cut(s)	658, 1088
EagI	CGGCCG	1 cut(s)	658
Eam1104I	CTCTTC	3 cut(s)	164, 1155, 1495
EarI	CTCTTC	3 cut(s)	164, 1155, 1495
EciI	GGCGGA	2 cut(s)	440, 626
EclXI	CGGCCG	1 cut(s)	658
Eco130I	CCWWGG	3 cut(s)	977, 1238, 1536
Eco147I	AGGCCT	1 cut(s)	544
Eco24I	GRGCYC	2 cut(s)	401, 871
Eco47I	GGWCC	3 cut(s)	754, 776, 1328
Eco52I	CGGCCG	1 cut(s)	658
Eco57I	CTGAAG	1 cut(s)	482
Eco88I	CYCGRG	1 cut(s)	203
EcoO109I	RGGNCCY	1 cut(s)	1328
EcoRI	GAATTC	2 cut(s)	969, 1542
EcoRII	CCWGG	4 cut(s)	420, 1090, 1225, 1429
EcoT14I	CCWWGG	3 cut(s)	977, 1238, 1536
EcoT38I	GRGCYC	2 cut(s)	401, 871
ErhI	CCWWGG	3 cut(s)	977, 1238, 1536
Esp3I	CGTCTC	1 cut(s)	1042
FaeI	CATG	7 cut(s)	524, 730, 859, 883, 1115, 1174, 1242
FalI	AAGNNNNNCTT	2 cut(s)	909, 941
FaqI	GGGAC	2 cut(s)	500, 1008
FatI	CATG	7 cut(s)	520, 726, 855, 879, 1111, 1170, 1238
FauNDI	CATATG	1 cut(s)	1282
FblI	GTMKAC	1 cut(s)	1404
Fnu4HI	GCNGC	7 cut(s)	114, 698, 930, 1019, 1396, 1470, 1473
FokI	GGATG	2 cut(s)	872, 1493
FriOI	GRGCYC	2 cut(s)	401, 871
FseI	GGCCGGCC	1 cut(s)	664
Fsp4HI	GCNGC	7 cut(s)	114, 698, 930, 1019, 1396, 1470, 1473
FspBI	CTAG	2 cut(s)	1131, 1157
GlaI	GCGC	1 cut(s)	644
GluI	GCNGC	7 cut(s)	114, 698, 930, 1019, 1396, 1470, 1473
GsaI	CCCAGC	1 cut(s)	819
GsuI	CTGGAG	3 cut(s)	260, 476, 1436
HaeII	RGCGCY	1 cut(s)	646
HgaI	GACGC	2 cut(s)	1005, 1059
HhaI	GCGC	1 cut(s)	645
Hin1II	CATG	7 cut(s)	524, 730, 859, 883, 1115, 1174, 1242
Hin6I	GCGC	1 cut(s)	643
HinP1I	GCGC	1 cut(s)	643
HinfI	GANTC	7 cut(s)	89, 304, 578, 874, 949, 1108, 1401
HphI	GGTGA	3 cut(s)	418, 457, 860
Hpy166II	GTNNAC	4 cut(s)	886, 1304, 1320, 1405
Hpy188I	TCNGA	7 cut(s)	199, 309, 583, 873, 1107, 1141, 1275
Hpy188III	TCNNGA	6 cut(s)	298, 362, 379, 727, 1157, 1363
Hpy8I	GTNNAC	4 cut(s)	886, 1304, 1320, 1405
HpyAV	CCTTC	6 cut(s)	34, 328, 376, 457, 550, 1486
HpyCH4III	ACNGT	1 cut(s)	1371
HpyCH4IV	ACGT	1 cut(s)	836
HpyCH4V	TGCA	2 cut(s)	605, 1475
HpyF10VI	GCNNNNNNNGC	7 cut(s)	514, 706, 812, 935, 1050, 1469, 1481
HpyF3I	CTNAG	7 cut(s)	143, 678, 1057, 1199, 1250, 1391, 1521
HpySE526I	ACGT	1 cut(s)	836
Hsp92II	CATG	7 cut(s)	524, 730, 859, 883, 1115, 1174, 1242
HspAI	GCGC	1 cut(s)	643
KroI	GCCGGC	1 cut(s)	660
KroNI	GCCGGC	1 cut(s)	662
KspI	CCGCGG	1 cut(s)	114
Kzo9I	GATC	2 cut(s)	1165, 1270
LmnI	GCTCC	6 cut(s)	241, 457, 732, 784, 874, 1417
Lsp1109I	GCAGC	4 cut(s)	1030, 1407, 1456, 1459
LweI	GCATC	3 cut(s)	225, 1456, 1471
MaeI	CTAG	2 cut(s)	1131, 1157
MaeII	ACGT	1 cut(s)	836
MalI	GATC	2 cut(s)	1167, 1272
MbiI	CCGCTC	2 cut(s)	244, 735
MboI	GATC	2 cut(s)	1165, 1270
MboII	GAAGA	9 cut(s)	88, 151, 241, 313, 326, 442, 1015, 1172, 1512
MhlI	GDGCHC	2 cut(s)	401, 871
MlsI	TGGCCA	1 cut(s)	1090
MluCI	AATT	8 cut(s)	130, 969, 1142, 1152, 1208, 1218, 1425, 1542
MluNI	TGGCCA	1 cut(s)	1090
MlyI	GAGTC	4 cut(s)	83, 587, 1117, 1410
MmeI	TCCRAC	2 cut(s)	762, 873
Mox20I	TGGCCA	1 cut(s)	1090
MroNI	GCCGGC	1 cut(s)	660
MroXI	GAANNNNTTC	1 cut(s)	386
MscI	TGGCCA	1 cut(s)	1090
MseI	TTAA	2 cut(s)	798, 1232
MslI	CAYNNNNRTG	1 cut(s)	233
Msp20I	TGGCCA	1 cut(s)	1090
MspA1I	CMGCKG	1 cut(s)	113
MvaI	CCWGG	4 cut(s)	422, 1092, 1227, 1431
MvnI	CGCG	3 cut(s)	113, 999, 1053
MwoI	GCNNNNNNNGC	7 cut(s)	514, 706, 812, 935, 1050, 1469, 1481
NaeI	GCCGGC	1 cut(s)	662
NciI	CCSGG	6 cut(s)	219, 272, 290, 392, 401, 657
NcoI	CCATGG	1 cut(s)	1238
NdeI	CATATG	1 cut(s)	1282
NdeII	GATC	2 cut(s)	1165, 1270
NgoMIV	GCCGGC	1 cut(s)	660
NlaIII	CATG	7 cut(s)	524, 730, 859, 883, 1115, 1174, 1242
NlaIV	GGNNCC	7 cut(s)	169, 516, 635, 870, 897, 984, 1298
NmeAIII	GCCGAG	2 cut(s)	576, 1424
NspI	RCATGY	2 cut(s)	524, 1174
NspV	TTCGAA	3 cut(s)	27, 327, 1099
PagI	TCATGA	1 cut(s)	726
PasI	CCCWGGG	1 cut(s)	1226
PceI	AGGCCT	1 cut(s)	544
PciI	ACATGT	1 cut(s)	520
PdiI	GCCGGC	1 cut(s)	662
PdmI	GAANNNNTTC	1 cut(s)	386
PfeI	GAWTC	3 cut(s)	304, 874, 949
PflMI	CCANNNNNTGG	2 cut(s)	238, 1414
PfoI	TCCNGGA	1 cut(s)	390
PinAI	ACCGGT	1 cut(s)	756
PkrI	GCNGC	7 cut(s)	115, 699, 931, 1020, 1397, 1471, 1474
PleI	GAGTC	4 cut(s)	83, 586, 1116, 1409
PpsI	GAGTC	4 cut(s)	83, 586, 1116, 1409
PpuMI	RGGWCCY	1 cut(s)	1328
PscI	ACATGT	1 cut(s)	520
Psp5II	RGGWCCY	1 cut(s)	1328
Psp6I	CCWGG	4 cut(s)	420, 1090, 1225, 1429
PspFI	CCCAGC	1 cut(s)	815
PspGI	CCWGG	4 cut(s)	420, 1090, 1225, 1429
PspN4I	GGNNCC	7 cut(s)	169, 516, 635, 870, 897, 984, 1298
PspPI	GGNCC	8 cut(s)	115, 167, 418, 530, 663, 754, 776, 1328
PspPPI	RGGWCCY	1 cut(s)	1328
PstNI	CAGNNNCTG	2 cut(s)	238, 1414
RigI	GGCCGGCC	1 cut(s)	664
RsaI	GTAC	3 cut(s)	482, 1065, 1319
RsaNI	GTAC	3 cut(s)	481, 1064, 1318
RseI	CAYNNNNRTG	1 cut(s)	233
SacII	CCGCGG	1 cut(s)	114
SaqAI	TTAA	2 cut(s)	798, 1232
SatI	GCNGC	7 cut(s)	114, 698, 930, 1019, 1396, 1470, 1473
Sau3AI	GATC	2 cut(s)	1165, 1270
Sau96I	GGNCC	8 cut(s)	115, 167, 418, 530, 663, 754, 776, 1328
ScaI	AGTACT	1 cut(s)	1065
SchI	GAGTC	4 cut(s)	83, 587, 1117, 1410
SduI	GDGCHC	2 cut(s)	401, 871
SfaNI	GCATC	3 cut(s)	225, 1456, 1471
SfcI	CTRYAG	1 cut(s)	1035
Sfr303I	CCGCGG	1 cut(s)	114
SfuI	TTCGAA	3 cut(s)	27, 327, 1099
SgrBI	CCGCGG	1 cut(s)	114
SinI	GGWCC	3 cut(s)	754, 776, 1328
SmiMI	CAYNNNNRTG	1 cut(s)	233
SmlI	CTYRAG	1 cut(s)	989
SmoI	CTYRAG	1 cut(s)	989
Sse9I	AATT	8 cut(s)	130, 969, 1142, 1152, 1208, 1218, 1425, 1542
SseBI	AGGCCT	1 cut(s)	544
SspI	AATATT	1 cut(s)	1378
SspMI	CTAG	2 cut(s)	1131, 1157
StuI	AGGCCT	1 cut(s)	544
StyI	CCWWGG	3 cut(s)	977, 1238, 1536
TaaI	ACNGT	1 cut(s)	1371
TaiI	ACGT	1 cut(s)	839
TaqI	TCGA	9 cut(s)	27, 297, 327, 669, 702, 766, 1099, 1342, 1383
TasI	AATT	8 cut(s)	130, 969, 1142, 1152, 1208, 1218, 1425, 1542
TatI	WGTACW	1 cut(s)	1063
TauI	GCSGC	3 cut(s)	116, 700, 932
TfiI	GAWTC	3 cut(s)	304, 874, 949
Tru1I	TTAA	2 cut(s)	798, 1232
Tru9I	TTAA	2 cut(s)	798, 1232
TscAI	CASTG	1 cut(s)	91
TseI	GCWGC	4 cut(s)	1018, 1395, 1469, 1472
TspDTI	ATGAA	3 cut(s)	829, 876, 1529
TspGWI	ACGGA	3 cut(s)	362, 768, 1289
TspRI	CASTG	1 cut(s)	91
Van91I	CCANNNNNTGG	2 cut(s)	238, 1414
VpaK11BI	GGWCC	3 cut(s)	754, 776, 1328
XapI	RAATTY	2 cut(s)	969, 1542
XbaI	TCTAGA	1 cut(s)	1156
XceI	RCATGY	2 cut(s)	524, 1174
XmiI	GTMKAC	1 cut(s)	1404
XmnI	GAANNNNTTC	1 cut(s)	386
XspI	CTAG	2 cut(s)	1131, 1157
ZrmI	AGTACT	1 cut(s)	1065

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.