Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000003

Orthogroup ID

OG0010035

Physical Location & Seq

Genomic Coordinates Forward (+)

4184403 .. 4187584

Gene Structure 3182 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug03G0054100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1659 bp

ATGCTCCTGGTCAAAACTCAAAGTTCAGAGCTTGAGAGAGAGAGAGAGAGTACTTTTTCCAAGAGTGATCTTTTGGGTAATGGCATGGCCATCCTCTCTTCCTTTCAAACCTTCACTCTCTCCCTAACACTCCTCTCCATCTTCCTCTTCTTCCTCCTCCTCACTCCCCCAACTCCCACTTCCCCAAACTACCCCTCCCCTTTCCCCCTAACCCCCCACAGGTCCCTCCTCCAAACTTCCTCCTCCTCCAATGGCCTCTTCAACTACCTCTGCCTCTCTCTCTCCTCCCTCTCTCTCATCCTCCTCCTCCTCTTCTACCTCCTCATCAAAACCGCCCAGGACCACTTCTCCCTCGTCACCGCCAAGCTCACCTTCGCCCTCAACCTGACGCCCTCCATGGCCGCCGTCACCCTCCTCGCCCTCGGCAACGGCGCCCCCGACGTCTTCGCCTCCCTCGCCGCCGTCCGCCGCGGAAACTACCGCACCGGCTTCGGCGCCATCCTCTCCGCCGGCACCTTCGTCTCCGCCTTCGTCGTCGGCTTCGTCGCGATCTACGCCGCGCCCTTCTCCCTCACCCCGGCCACGTTCGTCAGGGACGTCATGTTCTACCTGATCTCGGCCACGTTTCTGTTCTACGTGTATCTGAGCGCCGAGATTTATCTGTGGCAGGCGGTGGGGTTCGTCGGGTTTTACTTGTTCTTTGTGGGGTTTGTGTTCTGGATGGACTTGGGGTTGGGCGGAGGAGGAGGGAAGAGGAGCCGGAGTGAGGTGGCGGTGGGTGCTGAGAGTGAAATGCACAAGGGCTTTGTGGAATTGGATTGCGAGATCGGAGAAGTTGAGAAGAGATCAGAGGAGGGGAAGCCGAGTTTCGGGGTCCGGCGAGGTCTGGAAATGGTCTCAAAAGTATGGGAGGTTCCAGTCTCAATTCTTCTGAAGCTGACAATTCCACAGGCTGCCCCGTCGGAATGGAGTAGATTCTACACATCAGCCAATATTGCTCTATGCCCTCTAGCACTTCTATATTCTTGCAACTCGTTCATGGAATTCAATCATCCTGTAGCTTTTCTTCTTTCCAACACCCATCTTCCACTATGGTTTGTTGTATTCTTGGCAAGCTCCTCTCTTGCACTTCTTCATTATATAATAGAGAAAGAGCCCCCCAAAACTGAGCAAATTCCTGTATTGCTTATAGCATTTGTTATGAGTGTCTTTTGGATATCCACTACTGCTGGAGAATTGTTGAACTGCCTTGCTGCACTTGGAGCACTTCTGGAGTTGCCACCAGCACTTCTTGGGCTTACGGTTCTTGCCTGGGGAAATTCTGTGGGGGATCTTGTTGCTGATGTGGCACTTGCCAAGGCAGGCCACCCTGCATTGGCCATGGCAGGGTGCTTTGCCGGGCCAATGTTTAACATGCTTGTAGGCCTTGGAACAGCGTTGGTGATACAGACCTCCAATGTTTATCCGGAAGCATATGAGCTCCAGTTCCATGTGGGTATCGTGATTGCATTTGTGTTCTTGCTTTTGAGCCTGATGGGTTCTCTATTGGTGATCACATGGTGCAGATTCCGGGTGCCTAGGTTCTGGGGGTTCTGTCTTGTTGGTCTCTATGTTATCTTCATGGCAGTTAGCTTACTGATTGCGAAGTTTTCGGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006605 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0008565 GO:0009507 GO:0009526 GO:0009528 GO:0009536 GO:0009706 GO:0009941 GO:0015031 GO:0015833 GO:0016020 GO:0017038 GO:0019866 GO:0031090 GO:0031967 GO:0031969 GO:0031975 GO:0033036 GO:0033365 GO:0034613 GO:0042170 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045036 GO:0045037 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0065002 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0072594 GO:0072596 GO:0072598

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF4339

Protein Analysis

552

Amino Acids

60.16

Weight (kDa)

6.26

Isoelectric Point (pI)

43.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_Ca_ex	PF01699	98 - 240	1.1e-19	Sodium/calcium exchanger protein
Na_Ca_ex	PF01699	395 - 545	2.6e-29	Sodium/calcium exchanger protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010035)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01590
fragaria_vesca	FvH4_6g10790
malus_domestica	MD04G1164300.v1.1 MD12G1177900.v1.1
prunus_persica	Prupe.6G283400_v2.0.a1
pyrus_communis	pycom12g16830
rosa_chinensis	RchiOBHm_Chr3g0461841
rosa_laevigata	RLG00000024887 RLG00000024893
rosa_multiflora	Rmu_sc0004308.1_g000016 Rmu_sc0004308.1_g000019 Rmu_sc0007777.1_g000004 Rmu_sc0007777.1_g000014 Rmu_sc0007777.1_g000021
rosa_roxburghii	Rroxscaffold_6G00417870
rosa_rugosa	Rorug03G0054100 Rorug03G0054300
rosa_samantha	Rh3AG112200 Rh3CG117600 Rh3DG356900
rosa_wichuraiana	Rw3G009510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	444, 600
AccB1I	GGYRCC	4 cut(s)	431, 494, 512, 1573
AccII	CGCG	3 cut(s)	471, 548, 560
AccIII	TCCGGA	1 cut(s)	1465
AclWI	GGATC	1 cut(s)	1338
AcoI	YGGCCR	5 cut(s)	87, 399, 579, 618, 1377
AcsI	RAATTY	3 cut(s)	1043, 1173, 1318
AcuI	CTGAAG	1 cut(s)	953
AcyI	GRCGYC	5 cut(s)	389, 432, 441, 495, 597
AdeI	CACNNNGTG	1 cut(s)	1560
AfaI	GTAC	1 cut(s)	52
AfiI	CCNNNNNNNGG	7 cut(s)	219, 577, 616, 766, 869, 1375, 1386
AflIII	ACRYGT	1 cut(s)	636
AgsI	TTSAA	4 cut(s)	107, 262, 1048, 1243
AjnI	CCWGG	3 cut(s)	6, 336, 1310
AjuI	GAANNNNNNNTTGG	6 cut(s)	163, 195, 242, 274, 356, 388
AloI	GAACNNNNNNTCC	2 cut(s)	587, 619
AluBI	AGCT	7 cut(s)	31, 367, 937, 1061, 1116, 1480, 1632
AluI	AGCT	7 cut(s)	31, 367, 937, 1061, 1116, 1480, 1632
Alw21I	GWGCWC	2 cut(s)	1267, 1482
Alw26I	GTCTC	4 cut(s)	526, 901, 925, 1610
AlwI	GGATC	1 cut(s)	1338
Aor13HI	TCCGGA	1 cut(s)	1465
AoxI	GGCC	9 cut(s)	87, 253, 399, 579, 618, 1363, 1377, 1400, 1423
ApeKI	GCWGC	2 cut(s)	953, 1253
ApoI	RAATTY	3 cut(s)	1043, 1173, 1318
AspA2I	CCTAGG	1 cut(s)	1577
AspLEI	GCGC	4 cut(s)	434, 497, 562, 650
AspS9I	GGNCC	4 cut(s)	222, 340, 874, 1400
AsuC2I	CCSGG	3 cut(s)	578, 1399, 1571
AsuHPI	GGTGA	6 cut(s)	349, 361, 400, 565, 1453, 1561
AvaII	GGWCC	3 cut(s)	222, 340, 874
AvrII	CCTAGG	1 cut(s)	1577
BalI	TGGCCA	2 cut(s)	89, 1379
BanI	GGYRCC	4 cut(s)	431, 494, 512, 1573
BanII	GRGCYC	2 cut(s)	1158, 1482
BarI	GAAGNNNNNNTAC	2 cut(s)	1050, 1082
BbsI	GAAGAC	1 cut(s)	436
Bbv12I	GWGCWC	2 cut(s)	1267, 1482
BbvI	GCAGC	2 cut(s)	940, 1240
BccI	CCATC	6 cut(s)	98, 146, 506, 715, 1089, 1528
BceAI	ACGGC	3 cut(s)	389, 445, 446
BcgI	CGANNNNNNTGC	2 cut(s)	472, 506
BciT130I	CCWGG	3 cut(s)	8, 338, 1312
BclI	TGATCA	1 cut(s)	1551
BcnI	CCSGG	3 cut(s)	578, 1399, 1571
BcoDI	GTCTC	4 cut(s)	526, 901, 925, 1610
BfaI	CTAG	2 cut(s)	1010, 1578
BfmI	CTRYAG	1 cut(s)	1056
BfoI	RGCGCY	3 cut(s)	435, 498, 651
BisI	GCNGC	6 cut(s)	402, 459, 469, 558, 954, 1254
BlnI	CCTAGG	1 cut(s)	1577
BlsI	GCNGC	6 cut(s)	403, 460, 470, 559, 955, 1255
BmcAI	AGTACT	1 cut(s)	52
Bme1390I	CCNGG	6 cut(s)	8, 338, 578, 1312, 1399, 1571
Bme18I	GGWCC	3 cut(s)	222, 340, 874
BmgT120I	GGNCC	4 cut(s)	222, 340, 874, 1400
BmiI	GGNNCC	8 cut(s)	224, 433, 496, 514, 758, 875, 915, 1575
BmrFI	CCNGG	6 cut(s)	8, 338, 578, 1312, 1399, 1571
BpiI	GAAGAC	1 cut(s)	436
BpmI	CTGGAG	3 cut(s)	1251, 1292, 1466
BpuEI	CTTGAG	1 cut(s)	53
BpuMI	CCSGG	3 cut(s)	578, 1399, 1571
BsaAI	YACGTR	1 cut(s)	637
BsaHI	GRCGYC	5 cut(s)	389, 432, 441, 495, 597
BsaI	GGTCTC	2 cut(s)	901, 1610
BsaWI	WCCGGW	1 cut(s)	1465
BsaXI	ACNNNNNCTCC	6 cut(s)	179, 209, 332, 362, 748, 778
Bsc4I	CCNNNNNNNGG	7 cut(s)	219, 577, 616, 766, 869, 1375, 1386
Bse118I	RCCGGY	2 cut(s)	485, 509
Bse1I	ACTGG	2 cut(s)	917, 1483
BseAI	TCCGGA	1 cut(s)	1465
BseBI	CCWGG	3 cut(s)	8, 338, 1312
BseGI	GGATG	5 cut(s)	90, 297, 498, 726, 1051
BseLI	CCNNNNNNNGG	7 cut(s)	219, 577, 616, 766, 869, 1375, 1386
BseMII	CTCAG	3 cut(s)	635, 774, 1158
BseNI	ACTGG	2 cut(s)	917, 1483
BseXI	GCAGC	2 cut(s)	940, 1240
BsgI	GTGCAG	2 cut(s)	1239, 1582
Bsh1236I	CGCG	3 cut(s)	471, 548, 560
BshFI	GGCC	9 cut(s)	89, 255, 401, 581, 620, 1365, 1379, 1402, 1425
BshNI	GGYRCC	4 cut(s)	431, 494, 512, 1573
BsiHKAI	GWGCWC	2 cut(s)	1267, 1482
BsiSI	CCGG	8 cut(s)	486, 510, 578, 760, 877, 1398, 1466, 1570
BslFI	GGGAC	2 cut(s)	208, 608
BslI	CCNNNNNNNGG	7 cut(s)	219, 577, 616, 766, 869, 1375, 1386
BsmAI	GTCTC	4 cut(s)	526, 901, 925, 1610
BsmBI	CGTCTC	1 cut(s)	526
BsmFI	GGGAC	2 cut(s)	208, 608
BsnI	GGCC	9 cut(s)	89, 255, 401, 581, 620, 1365, 1379, 1402, 1425
Bso31I	GGTCTC	2 cut(s)	901, 1610
Bsp1286I	GDGCHC	3 cut(s)	1158, 1267, 1482
Bsp13I	TCCGGA	1 cut(s)	1465
Bsp143I	GATC	7 cut(s)	67, 549, 612, 825, 845, 1330, 1551
Bsp19I	CCATGG	2 cut(s)	396, 1380
Bsp68I	TCGCGA	1 cut(s)	548
BspANI	GGCC	9 cut(s)	89, 255, 401, 581, 620, 1365, 1379, 1402, 1425
BspCNI	CTCAG	3 cut(s)	636, 775, 1159
BspEI	TCCGGA	1 cut(s)	1465
BspFNI	CGCG	3 cut(s)	471, 548, 560
BspLI	GGNNCC	8 cut(s)	224, 433, 496, 514, 758, 875, 915, 1575
BspPI	GGATC	1 cut(s)	1338
BspT107I	GGYRCC	4 cut(s)	431, 494, 512, 1573
BspTNI	GGTCTC	2 cut(s)	901, 1610
BsrFI	RCCGGY	2 cut(s)	485, 509
BsrI	ACTGG	2 cut(s)	917, 1483
BssAI	RCCGGY	2 cut(s)	485, 509
BssMI	GATC	7 cut(s)	67, 549, 612, 825, 845, 1330, 1551
BssNI	GRCGYC	5 cut(s)	389, 432, 441, 495, 597
BssT1I	CCWWGG	5 cut(s)	396, 1356, 1380, 1426, 1577
Bst2UI	CCWGG	3 cut(s)	8, 338, 1312
Bst4CI	ACNGT	1 cut(s)	1303
Bst6I	CTCTTC	6 cut(s)	103, 152, 263, 317, 746, 836
BstACI	GRCGYC	5 cut(s)	389, 432, 441, 495, 597
BstBAI	YACGTR	1 cut(s)	637
BstC8I	GCNNGC	4 cut(s)	511, 669, 1114, 1363
BstDEI	CTNAG	3 cut(s)	644, 783, 1167
BstDSI	CCRYGG	3 cut(s)	396, 469, 1380
BstF5I	GGATG	5 cut(s)	90, 297, 498, 726, 1051
BstFNI	CGCG	3 cut(s)	471, 548, 560
BstH2I	RGCGCY	3 cut(s)	435, 498, 651
BstHHI	GCGC	4 cut(s)	434, 497, 562, 650
BstKTI	GATC	7 cut(s)	70, 552, 615, 828, 848, 1333, 1554
BstMAI	GTCTC	4 cut(s)	526, 901, 925, 1610
BstMBI	GATC	7 cut(s)	67, 549, 612, 825, 845, 1330, 1551
BstMWI	GCNNNNNNNGC	6 cut(s)	455, 554, 779, 995, 1262, 1346
BstNI	CCWGG	3 cut(s)	8, 338, 1312
BstNSI	RCATGY	1 cut(s)	1417
BstSCI	CCNGG	6 cut(s)	6, 336, 576, 1310, 1397, 1569
BstSFI	CTRYAG	1 cut(s)	1056
BstUI	CGCG	3 cut(s)	471, 548, 560
BstV1I	GCAGC	2 cut(s)	940, 1240
BstV2I	GAAGAC	1 cut(s)	436
BstX2I	RGATCY	1 cut(s)	1330
BstYI	RGATCY	1 cut(s)	1330
BsuRI	GGCC	9 cut(s)	89, 255, 401, 581, 620, 1365, 1379, 1402, 1425
BtgI	CCRYGG	3 cut(s)	396, 469, 1380
BtsCI	GGATG	5 cut(s)	90, 297, 498, 726, 1051
BtuMI	TCGCGA	1 cut(s)	548
Cac8I	GCNNGC	4 cut(s)	511, 669, 1114, 1363
CfoI	GCGC	4 cut(s)	434, 497, 562, 650
Cfr10I	RCCGGY	2 cut(s)	485, 509
Cfr13I	GGNCC	4 cut(s)	222, 340, 874, 1400
Cfr42I	CCGCGG	1 cut(s)	472
CseI	GACGC	1 cut(s)	397
Csp6I	GTAC	1 cut(s)	51
CviAII	CATG	9 cut(s)	85, 397, 601, 1039, 1381, 1414, 1490, 1557, 1621
CviQI	GTAC	1 cut(s)	51
DdeI	CTNAG	3 cut(s)	644, 783, 1167
DinI	GGCGCC	2 cut(s)	433, 496
DpnI	GATC	7 cut(s)	69, 551, 614, 827, 847, 1332, 1553
DpnII	GATC	7 cut(s)	67, 549, 612, 825, 845, 1330, 1551
DraIII	CACNNNGTG	1 cut(s)	1560
EaeI	YGGCCR	5 cut(s)	87, 399, 579, 618, 1377
Eam1104I	CTCTTC	6 cut(s)	103, 152, 263, 317, 746, 836
EarI	CTCTTC	6 cut(s)	103, 152, 263, 317, 746, 836
EciI	GGCGGA	4 cut(s)	455, 496, 514, 753
Ecl136II	GAGCTC	1 cut(s)	1480
Eco130I	CCWWGG	5 cut(s)	396, 1356, 1380, 1426, 1577
Eco147I	AGGCCT	1 cut(s)	1425
Eco24I	GRGCYC	2 cut(s)	1158, 1482
Eco31I	GGTCTC	2 cut(s)	901, 1610
Eco32I	GATATC	1 cut(s)	1218
Eco47I	GGWCC	3 cut(s)	222, 340, 874
Eco53kI	GAGCTC	1 cut(s)	1480
Eco57I	CTGAAG	1 cut(s)	953
EcoICRI	GAGCTC	1 cut(s)	1480
EcoO109I	RGGNCCY	1 cut(s)	222
EcoRI	GAATTC	1 cut(s)	1043
EcoRII	CCWGG	3 cut(s)	6, 336, 1310
EcoRV	GATATC	1 cut(s)	1218
EcoT14I	CCWWGG	5 cut(s)	396, 1356, 1380, 1426, 1577
EcoT38I	GRGCYC	2 cut(s)	1158, 1482
EgeI	GGCGCC	2 cut(s)	433, 496
EheI	GGCGCC	2 cut(s)	433, 496
ErhI	CCWWGG	5 cut(s)	396, 1356, 1380, 1426, 1577
Esp3I	CGTCTC	1 cut(s)	526
FaeI	CATG	9 cut(s)	88, 400, 604, 1042, 1384, 1417, 1493, 1560, 1624
FalI	AAGNNNNNCTT	2 cut(s)	356, 388
FaqI	GGGAC	2 cut(s)	208, 608
FatI	CATG	9 cut(s)	84, 396, 600, 1038, 1380, 1413, 1489, 1556, 1620
FauNDI	CATATG	1 cut(s)	1474
FbaI	TGATCA	1 cut(s)	1551
Fnu4HI	GCNGC	6 cut(s)	402, 459, 469, 558, 954, 1254
FokI	GGATG	5 cut(s)	77, 284, 485, 733, 1038
FriOI	GRGCYC	2 cut(s)	1158, 1482
Fsp4HI	GCNGC	6 cut(s)	402, 459, 469, 558, 954, 1254
FspBI	CTAG	2 cut(s)	1010, 1578
GlaI	GCGC	4 cut(s)	433, 496, 561, 649
GluI	GCNGC	6 cut(s)	402, 459, 469, 558, 954, 1254
GsuI	CTGGAG	3 cut(s)	1251, 1292, 1466
HaeII	RGCGCY	3 cut(s)	435, 498, 651
HaeIII	GGCC	9 cut(s)	89, 255, 401, 581, 620, 1365, 1379, 1402, 1425
HapII	CCGG	8 cut(s)	486, 510, 578, 760, 877, 1398, 1466, 1570
HgaI	GACGC	1 cut(s)	397
HhaI	GCGC	4 cut(s)	434, 497, 562, 650
Hin1I	GRCGYC	5 cut(s)	389, 432, 441, 495, 597
Hin1II	CATG	9 cut(s)	88, 400, 604, 1042, 1384, 1417, 1493, 1560, 1624
Hin6I	GCGC	4 cut(s)	432, 495, 560, 648
HinP1I	GCGC	4 cut(s)	432, 495, 560, 648
HinfI	GANTC	2 cut(s)	975, 1566
HpaII	CCGG	8 cut(s)	486, 510, 578, 760, 877, 1398, 1466, 1570
HphI	GGTGA	6 cut(s)	349, 361, 400, 565, 1453, 1561
Hpy188I	TCNGA	6 cut(s)	28, 645, 830, 850, 933, 964
Hpy188III	TCNNGA	7 cut(s)	547, 718, 887, 1271, 1466, 1501, 1653
Hpy99I	CGWCG	6 cut(s)	443, 536, 539, 548, 686, 964
HpyAV	CCTTC	5 cut(s)	121, 382, 526, 538, 574
HpyCH4III	ACNGT	1 cut(s)	1303
HpyCH4IV	ACGT	5 cut(s)	441, 584, 597, 623, 636
HpyCH4V	TGCA	7 cut(s)	796, 1029, 1127, 1256, 1373, 1508, 1563
HpyF10VI	GCNNNNNNNGC	6 cut(s)	455, 554, 779, 995, 1262, 1346
HpyF3I	CTNAG	3 cut(s)	644, 783, 1167
HpySE526I	ACGT	5 cut(s)	441, 584, 597, 623, 636
Hsp92I	GRCGYC	5 cut(s)	389, 432, 441, 495, 597
Hsp92II	CATG	9 cut(s)	88, 400, 604, 1042, 1384, 1417, 1493, 1560, 1624
HspAI	GCGC	4 cut(s)	432, 495, 560, 648
KasI	GGCGCC	2 cut(s)	431, 494
Kpn2I	TCCGGA	1 cut(s)	1465
KroI	GCCGGC	1 cut(s)	509
KroNI	GCCGGC	1 cut(s)	511
Ksp22I	TGATCA	1 cut(s)	1551
KspI	CCGCGG	1 cut(s)	472
Kzo9I	GATC	7 cut(s)	67, 549, 612, 825, 845, 1330, 1551
LmnI	GCTCC	5 cut(s)	9, 756, 1121, 1262, 1485
Lsp1109I	GCAGC	2 cut(s)	940, 1240
MaeI	CTAG	2 cut(s)	1010, 1578
MaeII	ACGT	5 cut(s)	441, 584, 597, 623, 636
MaeIII	GTNAC	2 cut(s)	355, 406
MalI	GATC	7 cut(s)	69, 551, 614, 827, 847, 1332, 1553
MboI	GATC	7 cut(s)	67, 549, 612, 825, 845, 1330, 1551
MflI	RGATCY	1 cut(s)	1330
MhlI	GDGCHC	3 cut(s)	1158, 1267, 1482
MlsI	TGGCCA	2 cut(s)	89, 1379
MluCI	AATT	7 cut(s)	812, 924, 942, 1043, 1173, 1235, 1318
MluNI	TGGCCA	2 cut(s)	89, 1379
Mly113I	GGCGCC	2 cut(s)	432, 495
MmeI	TCCRAC	2 cut(s)	942, 1098
Mox20I	TGGCCA	2 cut(s)	89, 1379
MroI	TCCGGA	1 cut(s)	1465
MroNI	GCCGGC	1 cut(s)	509
MscI	TGGCCA	2 cut(s)	89, 1379
MseI	TTAA	1 cut(s)	1410
Msp20I	TGGCCA	2 cut(s)	89, 1379
MspA1I	CMGCKG	1 cut(s)	471
MspI	CCGG	8 cut(s)	486, 510, 578, 760, 877, 1398, 1466, 1570
MspR9I	CCNGG	6 cut(s)	8, 338, 578, 1312, 1399, 1571
MvaI	CCWGG	3 cut(s)	8, 338, 1312
MvnI	CGCG	3 cut(s)	471, 548, 560
MwoI	GCNNNNNNNGC	6 cut(s)	455, 554, 779, 995, 1262, 1346
NaeI	GCCGGC	1 cut(s)	511
NarI	GGCGCC	2 cut(s)	432, 495
NciI	CCSGG	3 cut(s)	578, 1399, 1571
NcoI	CCATGG	2 cut(s)	396, 1380
NdeI	CATATG	1 cut(s)	1474
NdeII	GATC	7 cut(s)	67, 549, 612, 825, 845, 1330, 1551
NgoMIV	GCCGGC	1 cut(s)	509
NlaIII	CATG	9 cut(s)	88, 400, 604, 1042, 1384, 1417, 1493, 1560, 1624
NlaIV	GGNNCC	8 cut(s)	224, 433, 496, 514, 758, 875, 915, 1575
NmeAIII	GCCGAG	4 cut(s)	402, 596, 676, 888
NmuCI	GTSAC	2 cut(s)	355, 406
NruI	TCGCGA	1 cut(s)	548
NspI	RCATGY	1 cut(s)	1417
PceI	AGGCCT	1 cut(s)	1425
PcsI	WCGNNNNNNNCGW	3 cut(s)	435, 540, 594
PdiI	GCCGGC	1 cut(s)	511
PfeI	GAWTC	2 cut(s)	975, 1566
PkrI	GCNGC	6 cut(s)	403, 460, 470, 559, 955, 1255
PluTI	GGCGCC	2 cut(s)	435, 498
Ppu21I	YACGTR	1 cut(s)	637
PpuMI	RGGWCCY	1 cut(s)	222
Psp124BI	GAGCTC	1 cut(s)	1482
Psp5II	RGGWCCY	1 cut(s)	222
Psp6I	CCWGG	3 cut(s)	6, 336, 1310
PspGI	CCWGG	3 cut(s)	6, 336, 1310
PspN4I	GGNNCC	8 cut(s)	224, 433, 496, 514, 758, 875, 915, 1575
PspPI	GGNCC	4 cut(s)	222, 340, 874, 1400
PspPPI	RGGWCCY	1 cut(s)	222
PsrI	GAACNNNNNNTAC	2 cut(s)	897, 929
PsuI	RGATCY	1 cut(s)	1330
RruI	TCGCGA	1 cut(s)	548
RsaI	GTAC	1 cut(s)	52
RsaNI	GTAC	1 cut(s)	51
SacI	GAGCTC	1 cut(s)	1482
SacII	CCGCGG	1 cut(s)	472
SaqAI	TTAA	1 cut(s)	1410
SatI	GCNGC	6 cut(s)	402, 459, 469, 558, 954, 1254
Sau3AI	GATC	7 cut(s)	67, 549, 612, 825, 845, 1330, 1551
Sau96I	GGNCC	4 cut(s)	222, 340, 874, 1400
ScaI	AGTACT	1 cut(s)	52
ScrFI	CCNGG	6 cut(s)	8, 338, 578, 1312, 1399, 1571
SduI	GDGCHC	3 cut(s)	1158, 1267, 1482
SfcI	CTRYAG	1 cut(s)	1056
SfoI	GGCGCC	2 cut(s)	433, 496
Sfr303I	CCGCGG	1 cut(s)	472
SgrBI	CCGCGG	1 cut(s)	472
SinI	GGWCC	3 cut(s)	222, 340, 874
SmlI	CTYRAG	1 cut(s)	32
SmoI	CTYRAG	1 cut(s)	32
Sse9I	AATT	7 cut(s)	812, 924, 942, 1043, 1173, 1235, 1318
SseBI	AGGCCT	1 cut(s)	1425
SspDI	GGCGCC	2 cut(s)	431, 494
SspI	AATATT	1 cut(s)	994
SspMI	CTAG	2 cut(s)	1010, 1578
SstI	GAGCTC	1 cut(s)	1482
StuI	AGGCCT	1 cut(s)	1425
StyD4I	CCNGG	6 cut(s)	6, 336, 576, 1310, 1397, 1569
StyI	CCWWGG	5 cut(s)	396, 1356, 1380, 1426, 1577
TaaI	ACNGT	1 cut(s)	1303
TaiI	ACGT	5 cut(s)	444, 587, 600, 626, 639
TasI	AATT	7 cut(s)	812, 924, 942, 1043, 1173, 1235, 1318
TatI	WGTACW	1 cut(s)	50
TauI	GCSGC	4 cut(s)	404, 461, 471, 560
TfiI	GAWTC	2 cut(s)	975, 1566
Tru1I	TTAA	1 cut(s)	1410
Tru9I	TTAA	1 cut(s)	1410
TseFI	GTSAC	2 cut(s)	355, 406
TseI	GCWGC	2 cut(s)	953, 1253
Tsp45I	GTSAC	2 cut(s)	355, 406
TspDTI	ATGAA	3 cut(s)	1027, 1124, 1609
VpaK11BI	GGWCC	3 cut(s)	222, 340, 874
XapI	RAATTY	3 cut(s)	1043, 1173, 1318
XceI	RCATGY	1 cut(s)	1417
XmaJI	CCTAGG	1 cut(s)	1577
XspI	CTAG	2 cut(s)	1010, 1578
ZraI	GACGTC	2 cut(s)	442, 598
ZrmI	AGTACT	1 cut(s)	52

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.