MD12G1185700.v1.1

DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013954
Physical Location & Seq
Forward (+)
26720584 .. 26721741
1158 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1185700.v1.1.491

Sequence Viewer

Length: 1158 bp
ATGGGAAACCATAAGTTTAAATTATCAGATATGATGCCCCATTCCTGGTTTAGCAAGCTGAAAGACATGAGCAAACCCAGAAAAAAGCAATCCAAGAAGAAAAAACAACCCCCATTTGCTTCTTCTTCTCCAACAGCAAAATTTTTTACAGAGCCCTCAAAGCCAAAGCCGCAACTGCCCCACCACCTCCACTACCCCCCAAGACAGTCTTACTACTTCACAAGAGAGCTCACCTCCCCTGCGCCTGCCCCATCTCACGGATGCCCCACCTCCTCCTCCCCCACCAATGACCCACCACCAAAAAAATCCAACAGACAGAAACCGAGGAACAGAACTACCACCTTGCCCTCCTCCGATCCCAACCTCCTAACTTCCTCTGTTTCCGCCGGCTGCGGCTGCCGCTACACCACCATTGAATCCGTCTGGACTAGATCCCAATCCCCACCTGAGACAGGGTCTTCTTCCTCCTTCGACAGCTCTCCAGACCCGCCGTCAGAGACGGAGTTTCTCGACGACGACGATGAGCCTGAATTCCGGTGCGACCGCGTTCTCTCCGCTTACCATGCTGACTCCGGCGAAAAAGACGTCGTTATCGACGTCGAAAAGGCGTCACTGGCCATGAAATTATCCGATGTGCGAATTTCAGAATCGCCGGGTGATGGTTTGGATTCGTTTTCCGGGCTCGAGCTCCCTCCGATTATAACCAAGCCAGCAAAATTCAGCGAAATGGTGAGCGAAGTGAAAAAGAACAAGAACAAGAAGAAAGAGCAGAGGAATATTACTAACAGCGCTGGTAGAAGGCTTCCTTCATCAAATGCAGCTATATCTCCTGGCTTCCGCCTTCGGAATTCGCCGAGGATTTCCAACCGGAAGATCAACCAGGCCTGCCTCAGCCGGCGGAGCGTGTCGTCGAATTCGAGCTCGAAGCGGAGGAGCATCTCCGACAGCTTTGCAATTGTCAAGACATCGTTTGATCCCCAGAGGGATTTCAGGGAATCAATGGTGGAGATGATCATGGAGAACAACATCAAGGCGTCCAAGGATTTGGAAGATCTTCTTGCATGTTATCTTTCCTTGAATTCTGATGAGTACCATGAGATGATCATCAAGGTGTTCAAGCAAATCTGGTTTGATCTCACTGACATTGGGTCCAAGTAA

Protein Analysis

386

Amino Acids

43.15

Weight (kDa)

9.4

Isoelectric Point (pI)

71.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_binding_2 PF13724 1 - 33 1.2e-15 DNA-binding domain
Ovate PF04844 323 - 379 2.6e-25 Transcriptional repressor, ovate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013954)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01840
fragaria_vesca FvH4_6g10140
malus_domestica MD04G1172500.v1.1 MD12G1185700.v1.1
prunus_persica Prupe.6G290900_v2.0.a1
pyrus_communis pycom04g15240 pycom12g17430
rosa_chinensis RchiOBHm_Chr3g0460841
rosa_laevigata RLG00000024975
rosa_multiflora Rmu_sc0004055.1_g000001
rosa_roxburghii Rroxscaffold_6G00419170
rosa_rugosa Rorug03G0046900.1
rosa_samantha Rh3AG104000 Rh3BG107600 Rh3CG109500 Rh3DG109500
rosa_wichuraiana Rw3G008850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 701
AatII GACGTC 2 cut(s) 588, 600
AccII CGCG 1 cut(s) 546
AclWI GGATC 3 cut(s) 350, 426, 968
AcoI YGGCCR 1 cut(s) 615
AcsI RAATTY 7 cut(s) 140, 530, 639, 716, 847, 913, 1078
AcyI GRCGYC 4 cut(s) 585, 597, 608, 1034
AfaI GTAC 1 cut(s) 1091
AfeI AGCGCT 1 cut(s) 790
AfiI CCNNNNNNNGG 4 cut(s) 45, 257, 452, 659
AgsI TTSAA 3 cut(s) 416, 1078, 1117
AhdI GACNNNNNGTC 2 cut(s) 490, 1147
AjnI CCWGG 3 cut(s) 44, 829, 879
AluBI AGCT 7 cut(s) 58, 229, 477, 688, 821, 921, 948
AluI AGCT 7 cut(s) 58, 229, 477, 688, 821, 921, 948
Alw21I GWGCWC 3 cut(s) 231, 690, 923
Alw26I GTCTC 2 cut(s) 443, 491
AlwI GGATC 3 cut(s) 350, 426, 968
Ama87I CYCGRG 1 cut(s) 683
Aor51HI AGCGCT 1 cut(s) 790
AoxI GGCC 2 cut(s) 615, 882
ApeKI GCWGC 3 cut(s) 390, 396, 818
ApoI RAATTY 7 cut(s) 140, 530, 639, 716, 847, 913, 1078
ArsI GACNNNNNNTTYG 2 cut(s) 570, 602
Asp700I GAANNNNTTC 1 cut(s) 1053
AspLEI GCGC 2 cut(s) 244, 791
AspS9I GGNCC 1 cut(s) 1149
AsuC2I CCSGG 2 cut(s) 654, 679
AsuHPI GGTGA 3 cut(s) 223, 668, 742
AvaI CYCGRG 1 cut(s) 683
AvaII GGWCC 1 cut(s) 1149
BalI TGGCCA 1 cut(s) 617
BanII GRGCYC 5 cut(s) 156, 231, 684, 690, 923
BbsI GAAGAC 1 cut(s) 450
Bbv12I GWGCWC 3 cut(s) 231, 690, 923
BbvCI CCTCAGC 1 cut(s) 890
BbvI GCAGC 3 cut(s) 377, 383, 830
BccI CCATC 2 cut(s) 259, 653
BceAI ACGGC 1 cut(s) 475
BcgI CGANNNNNNTGC 2 cut(s) 932, 966
BciT130I CCWGG 3 cut(s) 46, 831, 881
BclI TGATCA 2 cut(s) 1011, 1101
BcnI CCSGG 2 cut(s) 654, 679
BcoDI GTCTC 2 cut(s) 443, 491
BfaI CTAG 1 cut(s) 429
BfoI RGCGCY 1 cut(s) 792
BglII AGATCT 1 cut(s) 1051
BisI GCNGC 6 cut(s) 170, 391, 394, 397, 400, 819
BlsI GCNGC 6 cut(s) 171, 392, 395, 398, 401, 820
Bme1390I CCNGG 5 cut(s) 46, 654, 679, 831, 881
Bme18I GGWCC 1 cut(s) 1149
BmeRI GACNNNNNGTC 2 cut(s) 490, 1147
BmeT110I CYCGRG 1 cut(s) 683
BmgT120I GGNCC 1 cut(s) 1149
BmiI GGNNCC 1 cut(s) 1150
BmrFI CCNGG 5 cut(s) 46, 654, 679, 831, 881
BmsI GCATC 3 cut(s) 24, 251, 945
BpiI GAAGAC 1 cut(s) 450
BplI GAGNNNNNCTC 2 cut(s) 218, 250
BpmI CTGGAG 1 cut(s) 465
Bpu10I CCTNAGC 1 cut(s) 890
BpuMI CCSGG 2 cut(s) 654, 679
BsaBI GATNNNNATC 2 cut(s) 436, 1103
BsaHI GRCGYC 4 cut(s) 585, 597, 608, 1034
BsaJI CCNNGG 3 cut(s) 323, 854, 1038
BsaWI WCCGGW 2 cut(s) 534, 867
Bsc4I CCNNNNNNNGG 4 cut(s) 45, 257, 452, 659
Bse118I RCCGGY 2 cut(s) 386, 894
Bse1I ACTGG 1 cut(s) 618
Bse8I GATNNNNATC 2 cut(s) 436, 1103
BseBI CCWGG 3 cut(s) 46, 831, 881
BseDI CCNNGG 3 cut(s) 323, 854, 1038
BseGI GGATG 1 cut(s) 266
BseJI GATNNNNATC 2 cut(s) 436, 1103
BseLI CCNNNNNNNGG 4 cut(s) 45, 257, 452, 659
BseMII CTCAG 2 cut(s) 438, 904
BseNI ACTGG 1 cut(s) 618
BseRI GAGGAG 4 cut(s) 262, 265, 340, 946
BseXI GCAGC 3 cut(s) 377, 383, 830
Bsh1236I CGCG 1 cut(s) 546
Bsh1285I CGRYCG 1 cut(s) 544
BshFI GGCC 2 cut(s) 617, 884
BsiEI CGRYCG 1 cut(s) 544
BsiHKAI GWGCWC 3 cut(s) 231, 690, 923
BsiHKCI CYCGRG 1 cut(s) 683
BsiSI CCGG 7 cut(s) 387, 535, 573, 653, 678, 868, 895
BslI CCNNNNNNNGG 4 cut(s) 45, 257, 452, 659
BsmAI GTCTC 2 cut(s) 443, 491
BsmBI CGTCTC 1 cut(s) 491
BsnI GGCC 2 cut(s) 617, 884
BsoBI CYCGRG 1 cut(s) 683
Bsp1286I GDGCHC 5 cut(s) 156, 231, 684, 690, 923
Bsp143I GATC 8 cut(s) 355, 431, 873, 973, 1011, 1051, 1101, 1132
BspANI GGCC 2 cut(s) 617, 884
BspCNI CTCAG 2 cut(s) 439, 903
BspFNI CGCG 1 cut(s) 546
BspLI GGNNCC 1 cut(s) 1150
BspPI GGATC 3 cut(s) 350, 426, 968
BsrFI RCCGGY 2 cut(s) 386, 894
BsrI ACTGG 1 cut(s) 618
BssAI RCCGGY 2 cut(s) 386, 894
BssECI CCNNGG 3 cut(s) 323, 854, 1038
BssMI GATC 8 cut(s) 355, 431, 873, 973, 1011, 1051, 1101, 1132
BssNI GRCGYC 4 cut(s) 585, 597, 608, 1034
BssT1I CCWWGG 1 cut(s) 1038
Bst2UI CCWGG 3 cut(s) 46, 831, 881
Bst4CI ACNGT 1 cut(s) 207
BstACI GRCGYC 4 cut(s) 585, 597, 608, 1034
BstC8I GCNNGC 6 cut(s) 56, 246, 388, 711, 886, 896
BstDEI CTNAG 2 cut(s) 447, 890
BstENI CCTNNNNNAGG 1 cut(s) 450
BstF5I GGATG 1 cut(s) 266
BstFNI CGCG 1 cut(s) 546
BstH2I RGCGCY 1 cut(s) 792
BstHHI GCGC 2 cut(s) 244, 791
BstKTI GATC 8 cut(s) 358, 434, 876, 976, 1014, 1054, 1104, 1135
BstMAI GTCTC 2 cut(s) 443, 491
BstMBI GATC 8 cut(s) 355, 431, 873, 973, 1011, 1051, 1101, 1132
BstMCI CGRYCG 1 cut(s) 544
BstMWI GCNNNNNNNGC 8 cut(s) 160, 169, 175, 396, 399, 563, 614, 900
BstNI CCWGG 3 cut(s) 46, 831, 881
BstNSI RCATGY 1 cut(s) 1065
BstSCI CCNGG 5 cut(s) 44, 652, 677, 829, 879
BstUI CGCG 1 cut(s) 546
BstV1I GCAGC 3 cut(s) 377, 383, 830
BstV2I GAAGAC 1 cut(s) 450
BstX2I RGATCY 2 cut(s) 431, 1051
BstXI CCANNNNNNTGG 1 cut(s) 1045
BstYI RGATCY 2 cut(s) 431, 1051
BsuRI GGCC 2 cut(s) 617, 884
BtsCI GGATG 1 cut(s) 266
BtsIMutI CAGTG 2 cut(s) 611, 1137
Cac8I GCNNGC 6 cut(s) 56, 246, 388, 711, 886, 896
CfoI GCGC 2 cut(s) 244, 791
Cfr10I RCCGGY 2 cut(s) 386, 894
Cfr13I GGNCC 1 cut(s) 1149
CseI GACGC 2 cut(s) 597, 1023
Csp6I GTAC 1 cut(s) 1090
CviAII CATG 6 cut(s) 67, 563, 619, 1015, 1062, 1094
CviQI GTAC 1 cut(s) 1090
DdeI CTNAG 2 cut(s) 447, 890
DpnI GATC 8 cut(s) 357, 433, 875, 975, 1013, 1053, 1103, 1134
DpnII GATC 8 cut(s) 355, 431, 873, 973, 1011, 1051, 1101, 1132
DraI TTTAAA 1 cut(s) 19
DriI GACNNNNNGTC 2 cut(s) 490, 1147
EaeI YGGCCR 1 cut(s) 615
Eam1105I GACNNNNNGTC 2 cut(s) 490, 1147
EciI GGCGGA 3 cut(s) 373, 827, 913
Ecl136II GAGCTC 3 cut(s) 229, 688, 921
Eco130I CCWWGG 1 cut(s) 1038
Eco147I AGGCCT 1 cut(s) 884
Eco24I GRGCYC 5 cut(s) 156, 231, 684, 690, 923
Eco47I GGWCC 1 cut(s) 1149
Eco47III AGCGCT 1 cut(s) 790
Eco53kI GAGCTC 3 cut(s) 229, 688, 921
Eco88I CYCGRG 1 cut(s) 683
EcoICRI GAGCTC 3 cut(s) 229, 688, 921
EcoNI CCTNNNNNAGG 1 cut(s) 450
EcoRI GAATTC 4 cut(s) 530, 847, 913, 1078
EcoRII CCWGG 3 cut(s) 44, 829, 879
EcoT14I CCWWGG 1 cut(s) 1038
EcoT38I GRGCYC 5 cut(s) 156, 231, 684, 690, 923
ErhI CCWWGG 1 cut(s) 1038
Esp3I CGTCTC 1 cut(s) 491
FaeI CATG 6 cut(s) 70, 566, 622, 1018, 1065, 1097
FalI AAGNNNNNCTT 6 cut(s) 193, 225, 790, 822, 1041, 1073
FatI CATG 6 cut(s) 66, 562, 618, 1014, 1061, 1093
FauI CCCGC 1 cut(s) 495
FbaI TGATCA 2 cut(s) 1011, 1101
Fnu4HI GCNGC 6 cut(s) 170, 391, 394, 397, 400, 819
FokI GGATG 1 cut(s) 273
FriOI GRGCYC 5 cut(s) 156, 231, 684, 690, 923
Fsp4HI GCNGC 6 cut(s) 170, 391, 394, 397, 400, 819
FspBI CTAG 1 cut(s) 429
GlaI GCGC 2 cut(s) 243, 790
GluI GCNGC 6 cut(s) 170, 391, 394, 397, 400, 819
GsuI CTGGAG 1 cut(s) 465
HaeII RGCGCY 1 cut(s) 792
HaeIII GGCC 2 cut(s) 617, 884
HapII CCGG 7 cut(s) 387, 535, 573, 653, 678, 868, 895
HgaI GACGC 2 cut(s) 597, 1023
HhaI GCGC 2 cut(s) 244, 791
Hin1I GRCGYC 4 cut(s) 585, 597, 608, 1034
Hin1II CATG 6 cut(s) 70, 566, 622, 1018, 1065, 1097
Hin6I GCGC 2 cut(s) 242, 789
HinP1I GCGC 2 cut(s) 242, 789
HinfI GANTC 5 cut(s) 416, 569, 647, 668, 995
HpaII CCGG 7 cut(s) 387, 535, 573, 653, 678, 868, 895
HphI GGTGA 3 cut(s) 223, 668, 742
Hpy188I TCNGA 9 cut(s) 28, 355, 496, 631, 646, 696, 846, 943, 1084
Hpy188III TCNNGA 4 cut(s) 424, 482, 509, 961
Hpy99I CGWCG 7 cut(s) 515, 518, 521, 590, 599, 602, 913
HpyAV CCTTC 4 cut(s) 478, 792, 816, 851
HpyCH4III ACNGT 1 cut(s) 207
HpyCH4IV ACGT 2 cut(s) 585, 597
HpyCH4V TGCA 3 cut(s) 818, 953, 1061
HpyF10VI GCNNNNNNNGC 8 cut(s) 160, 169, 175, 396, 399, 563, 614, 900
HpyF3I CTNAG 2 cut(s) 447, 890
HpySE526I ACGT 2 cut(s) 585, 597
Hsp92I GRCGYC 4 cut(s) 585, 597, 608, 1034
Hsp92II CATG 6 cut(s) 70, 566, 622, 1018, 1065, 1097
HspAI GCGC 2 cut(s) 242, 789
KroI GCCGGC 2 cut(s) 386, 894
KroNI GCCGGC 2 cut(s) 388, 896
Ksp22I TGATCA 2 cut(s) 1011, 1101
Kzo9I GATC 8 cut(s) 355, 431, 873, 973, 1011, 1051, 1101, 1132
LmnI GCTCC 3 cut(s) 693, 900, 933
Lsp1109I GCAGC 3 cut(s) 377, 383, 830
LweI GCATC 3 cut(s) 24, 251, 945
MaeI CTAG 1 cut(s) 429
MaeII ACGT 2 cut(s) 585, 597
MaeIII GTNAC 1 cut(s) 609
MalI GATC 8 cut(s) 357, 433, 875, 975, 1013, 1053, 1103, 1134
MboI GATC 8 cut(s) 355, 431, 873, 973, 1011, 1051, 1101, 1132
MboII GAAGA 9 cut(s) 109, 114, 117, 450, 453, 772, 883, 1046, 1061
MfeI CAATTG 1 cut(s) 954
MflI RGATCY 2 cut(s) 431, 1051
MhlI GDGCHC 5 cut(s) 156, 231, 684, 690, 923
MlsI TGGCCA 1 cut(s) 617
MluNI TGGCCA 1 cut(s) 617
MlyI GAGTC 1 cut(s) 563
MmeI TCCRAC 4 cut(s) 155, 333, 888, 966
Mox20I TGGCCA 1 cut(s) 617
MroNI GCCGGC 2 cut(s) 386, 894
MroXI GAANNNNTTC 1 cut(s) 1053
MscI TGGCCA 1 cut(s) 617
MseI TTAA 1 cut(s) 18
MslI CAYNNNNRTG 1 cut(s) 1109
Msp20I TGGCCA 1 cut(s) 617
MspI CCGG 7 cut(s) 387, 535, 573, 653, 678, 868, 895
MspR9I CCNGG 5 cut(s) 46, 654, 679, 831, 881
MunI CAATTG 1 cut(s) 954
MvaI CCWGG 3 cut(s) 46, 831, 881
MvnI CGCG 1 cut(s) 546
MwoI GCNNNNNNNGC 8 cut(s) 160, 169, 175, 396, 399, 563, 614, 900
NaeI GCCGGC 2 cut(s) 388, 896
NciI CCSGG 2 cut(s) 654, 679
NdeII GATC 8 cut(s) 355, 431, 873, 973, 1011, 1051, 1101, 1132
NgoMIV GCCGGC 2 cut(s) 386, 894
NlaIII CATG 6 cut(s) 70, 566, 622, 1018, 1065, 1097
NlaIV GGNNCC 1 cut(s) 1150
NmeAIII GCCGAG 1 cut(s) 879
NmuCI GTSAC 1 cut(s) 609
NspI RCATGY 1 cut(s) 1065
PaeR7I CTCGAG 1 cut(s) 683
PceI AGGCCT 1 cut(s) 884
PcsI WCGNNNNNNNCGW 4 cut(s) 516, 591, 594, 914
PdiI GCCGGC 2 cut(s) 388, 896
PdmI GAANNNNTTC 1 cut(s) 1053
PfeI GAWTC 4 cut(s) 416, 647, 668, 995
PflFI GACNNNGTC 1 cut(s) 454
PkrI GCNGC 6 cut(s) 171, 392, 395, 398, 401, 820
PleI GAGTC 1 cut(s) 563
PpsI GAGTC 1 cut(s) 563
PsiI TTATAA 1 cut(s) 701
Psp124BI GAGCTC 3 cut(s) 231, 690, 923
Psp6I CCWGG 3 cut(s) 44, 829, 879
PspGI CCWGG 3 cut(s) 44, 829, 879
PspN4I GGNNCC 1 cut(s) 1150
PspPI GGNCC 1 cut(s) 1149
PspXI VCTCGAGB 1 cut(s) 683
PsuI RGATCY 2 cut(s) 431, 1051
PsyI GACNNNGTC 1 cut(s) 454
RsaI GTAC 1 cut(s) 1091
RsaNI GTAC 1 cut(s) 1090
RseI CAYNNNNRTG 1 cut(s) 1109
SacI GAGCTC 3 cut(s) 231, 690, 923
SaqAI TTAA 1 cut(s) 18
SatI GCNGC 6 cut(s) 170, 391, 394, 397, 400, 819
Sau3AI GATC 8 cut(s) 355, 431, 873, 973, 1011, 1051, 1101, 1132
Sau96I GGNCC 1 cut(s) 1149
SchI GAGTC 1 cut(s) 563
ScrFI CCNGG 5 cut(s) 46, 654, 679, 831, 881
SduI GDGCHC 5 cut(s) 156, 231, 684, 690, 923
SfaNI GCATC 3 cut(s) 24, 251, 945
Sfr274I CTCGAG 1 cut(s) 683
SinI GGWCC 1 cut(s) 1149
SlaI CTCGAG 1 cut(s) 683
SmiMI CAYNNNNRTG 1 cut(s) 1109
SmlI CTYRAG 1 cut(s) 683
SmoI CTYRAG 1 cut(s) 683
SseBI AGGCCT 1 cut(s) 884
SspI AATATT 1 cut(s) 778
SspMI CTAG 1 cut(s) 429
SstI GAGCTC 3 cut(s) 231, 690, 923
StuI AGGCCT 1 cut(s) 884
StyD4I CCNGG 5 cut(s) 44, 652, 677, 829, 879
StyI CCWWGG 1 cut(s) 1038
TaaI ACNGT 1 cut(s) 207
TaiI ACGT 2 cut(s) 588, 600
TaqI TCGA 8 cut(s) 471, 510, 594, 600, 684, 911, 917, 923
TauI GCSGC 3 cut(s) 172, 396, 402
TfiI GAWTC 4 cut(s) 416, 647, 668, 995
Tru1I TTAA 1 cut(s) 18
Tru9I TTAA 1 cut(s) 18
TscAI CASTG 2 cut(s) 618, 1144
TseFI GTSAC 1 cut(s) 609
TseI GCWGC 3 cut(s) 390, 396, 818
Tsp45I GTSAC 1 cut(s) 609
TspDTI ATGAA 2 cut(s) 635, 798
TspGWI ACGGA 3 cut(s) 273, 409, 515
TspRI CASTG 2 cut(s) 618, 1144
Tth111I GACNNNGTC 1 cut(s) 454
VpaK11BI GGWCC 1 cut(s) 1149
XagI CCTNNNNNAGG 1 cut(s) 450
XapI RAATTY 7 cut(s) 140, 530, 639, 716, 847, 913, 1078
XceI RCATGY 1 cut(s) 1065
XhoI CTCGAG 1 cut(s) 683
XmnI GAANNNNTTC 1 cut(s) 1053
XspI CTAG 1 cut(s) 429
ZraI GACGTC 2 cut(s) 586, 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.