pycom12g17430

DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0013954
Physical Location & Seq
Forward (+)
19796573 .. 19797739
1167 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g17430.1

Sequence Viewer

Length: 1167 bp
ATGGGAAACCACAAGTTTAAATTATCAGATATGATGCCCAATTCCTGGTTTAGCAAGTTGAAAGACATGAGCAAACCCAGAAAAAAGCAATCCAAGAAGAAAAAACAACAAAAACAACCCCCATTTGCTGCTTCTTCTCCACCAGCAAAAAAGTTTACAGAGCCCTCAAAGCCAAAGCTGCAACTGCCCCACCACCTCCACTACCCTCCAAGACAGTCTTACTACTTCACAAGAGAGCTCACCTCCCCTGCGCCTGCCCCATCTCACGGATGCCCCACCTCCTCCTCCCCCACCAATGACCCACCACCAAAAAAATCCAACAGACAAAAACCGAGGAACAGAACTACCACCTTGCCCTCCTCCGATCCCAACCTCCGAACTTCCTCTGTTTCCGCCGGCTGCGGCTGCCACTCCACCACCATTGAATCCGTCTGGACTAGATCCCAATCCCCACCTGGGACATGGTCGTCTTCCTCCTTCGACAGCTCTCCAGACCCGCCGTCAGAGACGGAGTTTCTCGACGACGACGATGAGCCTGAATTCCGGTGCGACCGCGTTCTCTCCGCTTACCATGTTGACTCCGGCGAAAAAGACGTCGTTGTCGACGTCGAAAGGGCGTCACTGGCCATGAAATTATCCGATGTGAGAATTGTAGATGCGCCTGGTGATGGTTTGGATTCGTTTTCCGGGCTCGATCTCCCTCCGATTATAACCAAGCCAGCAAAATTCAGCAAAATGGTGAGCGAAGTGAAGAAGAGCAAGAACAAGAAGAAAGAACAGAGGAATATTACCAACAGCGCTGGTAGAAGGCTTCCTTCATCAAATGCAGCTATATCTCCCGGCTTCCGCCTTCGGAATTCGCCGAGGATTTCCAACCGGAAGATCAACCAGGCCTGCCTCAGCCGGCGGAGCGTGTCGTCGAATTCGAGCTCGAAGCGGAGGAGCATCTCCGACAGCTTTGCAATTGTCAAGTCATCGTTTGATCCCCAGAGGGATTTCAGGGAATCAATGGTGGAGATGATCATGGAGAACAACATCAAGGCGTCCAAGGATATGGAAGATCTACTTGCATGTTATCTTTCTTTAAATTCTGATGAGTACCATGAGATGATCATCAAGGTGTTCAAGCAAATCTGGTTTGATCTCACTGACATTGGGTCTAAGTAA

Protein Analysis

389

Amino Acids

43.47

Weight (kDa)

9.62

Isoelectric Point (pI)

71.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_binding_2 PF13724 1 - 38 5.2e-17 DNA-binding domain
Ovate PF04844 326 - 382 3.9e-25 Transcriptional repressor, ovate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013954)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01840
fragaria_vesca FvH4_6g10140
malus_domestica MD04G1172500.v1.1 MD12G1185700.v1.1
prunus_persica Prupe.6G290900_v2.0.a1
pyrus_communis pycom04g15240 pycom12g17430
rosa_chinensis RchiOBHm_Chr3g0460841
rosa_laevigata RLG00000024975
rosa_multiflora Rmu_sc0004055.1_g000001
rosa_roxburghii Rroxscaffold_6G00419170
rosa_rugosa Rorug03G0046900.1
rosa_samantha Rh3AG104000 Rh3BG107600 Rh3CG109500 Rh3DG109500
rosa_wichuraiana Rw3G008850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 710
AasI GACNNNNNNGTC 2 cut(s) 466, 599
AatII GACGTC 2 cut(s) 597, 609
AccB7I CCANNNNNTGG 1 cut(s) 45
AccI GTMKAC 1 cut(s) 603
AccII CGCG 1 cut(s) 555
AciI CCGC 8 cut(s) 393, 402, 497, 553, 564, 847, 907, 937
AclWI GGATC 3 cut(s) 359, 435, 977
AcoI YGGCCR 1 cut(s) 624
AcsI RAATTY 5 cut(s) 539, 725, 856, 922, 1087
AcyI GRCGYC 4 cut(s) 594, 606, 617, 1043
AfaI GTAC 1 cut(s) 1100
AfeI AGCGCT 1 cut(s) 799
AfiI CCNNNNNNNGG 4 cut(s) 45, 266, 456, 668
AgsI TTSAA 3 cut(s) 61, 425, 1126
AhdI GACNNNNNGTC 2 cut(s) 499, 1156
AjnI CCWGG 4 cut(s) 44, 454, 661, 888
AluBI AGCT 6 cut(s) 178, 238, 486, 830, 930, 957
AluI AGCT 6 cut(s) 178, 238, 486, 830, 930, 957
Alw21I GWGCWC 2 cut(s) 240, 932
Alw26I GTCTC 1 cut(s) 500
AlwI GGATC 3 cut(s) 359, 435, 977
Aor51HI AGCGCT 1 cut(s) 799
AoxI GGCC 2 cut(s) 624, 891
ApeKI GCWGC 5 cut(s) 128, 178, 399, 405, 827
ApoI RAATTY 5 cut(s) 539, 725, 856, 922, 1087
ArsI GACNNNNNNTTYG 2 cut(s) 579, 611
AspLEI GCGC 3 cut(s) 253, 661, 800
AsuC2I CCSGG 2 cut(s) 688, 840
AsuHPI GGTGA 3 cut(s) 232, 677, 751
BalI TGGCCA 1 cut(s) 626
BanII GRGCYC 4 cut(s) 165, 240, 693, 932
BbsI GAAGAC 1 cut(s) 462
Bbv12I GWGCWC 2 cut(s) 240, 932
BbvCI CCTCAGC 1 cut(s) 899
BbvI GCAGC 5 cut(s) 115, 165, 386, 392, 839
BccI CCATC 2 cut(s) 268, 662
BceAI ACGGC 1 cut(s) 484
BcgI CGANNNNNNTGC 2 cut(s) 941, 975
BciT130I CCWGG 4 cut(s) 46, 456, 663, 890
BclI TGATCA 2 cut(s) 1020, 1110
BcnI CCSGG 2 cut(s) 688, 840
BcoDI GTCTC 1 cut(s) 500
BfaI CTAG 1 cut(s) 438
BfoI RGCGCY 1 cut(s) 801
BglII AGATCT 1 cut(s) 1060
BisI GCNGC 6 cut(s) 129, 179, 400, 403, 406, 828
BlsI GCNGC 6 cut(s) 130, 180, 401, 404, 407, 829
Bme1390I CCNGG 6 cut(s) 46, 456, 663, 688, 840, 890
BmeRI GACNNNNNGTC 2 cut(s) 499, 1156
BmrFI CCNGG 6 cut(s) 46, 456, 663, 688, 840, 890
BmsI GCATC 4 cut(s) 24, 260, 646, 954
BpiI GAAGAC 1 cut(s) 462
BplI GAGNNNNNCTC 2 cut(s) 227, 259
BpmI CTGGAG 1 cut(s) 474
Bpu10I CCTNAGC 1 cut(s) 899
BpuMI CCSGG 2 cut(s) 688, 840
BsaBI GATNNNNATC 2 cut(s) 445, 1112
BsaHI GRCGYC 4 cut(s) 594, 606, 617, 1043
BsaJI CCNNGG 4 cut(s) 332, 455, 863, 1047
BsaWI WCCGGW 2 cut(s) 543, 876
Bsc4I CCNNNNNNNGG 4 cut(s) 45, 266, 456, 668
Bse118I RCCGGY 2 cut(s) 395, 903
Bse1I ACTGG 1 cut(s) 627
Bse8I GATNNNNATC 2 cut(s) 445, 1112
BseBI CCWGG 4 cut(s) 46, 456, 663, 890
BseDI CCNNGG 4 cut(s) 332, 455, 863, 1047
BseGI GGATG 1 cut(s) 275
BseJI GATNNNNATC 2 cut(s) 445, 1112
BseLI CCNNNNNNNGG 4 cut(s) 45, 266, 456, 668
BseMII CTCAG 1 cut(s) 913
BseNI ACTGG 1 cut(s) 627
BseRI GAGGAG 4 cut(s) 271, 274, 349, 955
BseXI GCAGC 5 cut(s) 115, 165, 386, 392, 839
Bsh1236I CGCG 1 cut(s) 555
Bsh1285I CGRYCG 1 cut(s) 553
BshFI GGCC 2 cut(s) 626, 893
BsiEI CGRYCG 1 cut(s) 553
BsiHKAI GWGCWC 2 cut(s) 240, 932
BsiSI CCGG 7 cut(s) 396, 544, 582, 687, 840, 877, 904
BslFI GGGAC 1 cut(s) 472
BslI CCNNNNNNNGG 4 cut(s) 45, 266, 456, 668
BsmAI GTCTC 1 cut(s) 500
BsmBI CGTCTC 1 cut(s) 500
BsmFI GGGAC 1 cut(s) 472
BsnI GGCC 2 cut(s) 626, 893
Bsp1286I GDGCHC 4 cut(s) 165, 240, 693, 932
Bsp143I GATC 9 cut(s) 364, 440, 694, 882, 982, 1020, 1060, 1110, 1141
BspACI CCGC 8 cut(s) 393, 402, 497, 553, 564, 847, 907, 937
BspANI GGCC 2 cut(s) 626, 893
BspCNI CTCAG 1 cut(s) 912
BspFNI CGCG 1 cut(s) 555
BspPI GGATC 3 cut(s) 359, 435, 977
BspQI GCTCTTC 1 cut(s) 749
BsrFI RCCGGY 2 cut(s) 395, 903
BsrI ACTGG 1 cut(s) 627
BssAI RCCGGY 2 cut(s) 395, 903
BssECI CCNNGG 4 cut(s) 332, 455, 863, 1047
BssMI GATC 9 cut(s) 364, 440, 694, 882, 982, 1020, 1060, 1110, 1141
BssNI GRCGYC 4 cut(s) 594, 606, 617, 1043
BssT1I CCWWGG 1 cut(s) 1047
Bst2UI CCWGG 4 cut(s) 46, 456, 663, 890
Bst4CI ACNGT 1 cut(s) 216
Bst6I CTCTTC 1 cut(s) 749
BstACI GRCGYC 4 cut(s) 594, 606, 617, 1043
BstC8I GCNNGC 5 cut(s) 255, 397, 720, 895, 905
BstDEI CTNAG 2 cut(s) 899, 1161
BstF5I GGATG 1 cut(s) 275
BstFNI CGCG 1 cut(s) 555
BstH2I RGCGCY 1 cut(s) 801
BstHHI GCGC 3 cut(s) 253, 661, 800
BstKTI GATC 9 cut(s) 367, 443, 697, 885, 985, 1023, 1063, 1113, 1144
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 9 cut(s) 364, 440, 694, 882, 982, 1020, 1060, 1110, 1141
BstMCI CGRYCG 1 cut(s) 553
BstMWI GCNNNNNNNGC 6 cut(s) 169, 178, 184, 405, 623, 909
BstNI CCWGG 4 cut(s) 46, 456, 663, 890
BstNSI RCATGY 1 cut(s) 1074
BstSCI CCNGG 6 cut(s) 44, 454, 661, 686, 838, 888
BstUI CGCG 1 cut(s) 555
BstV1I GCAGC 5 cut(s) 115, 165, 386, 392, 839
BstV2I GAAGAC 1 cut(s) 462
BstX2I RGATCY 2 cut(s) 440, 1060
BstXI CCANNNNNNTGG 1 cut(s) 1054
BstYI RGATCY 2 cut(s) 440, 1060
BsuRI GGCC 2 cut(s) 626, 893
BtsCI GGATG 1 cut(s) 275
BtsIMutI CAGTG 2 cut(s) 620, 1146
Cac8I GCNNGC 5 cut(s) 255, 397, 720, 895, 905
CfoI GCGC 3 cut(s) 253, 661, 800
Cfr10I RCCGGY 2 cut(s) 395, 903
CseI GACGC 2 cut(s) 606, 1032
Csp6I GTAC 1 cut(s) 1099
CspCI CAANNNNNGTGG 2 cut(s) 403, 438
CviAII CATG 7 cut(s) 67, 462, 572, 628, 1024, 1071, 1103
CviQI GTAC 1 cut(s) 1099
DdeI CTNAG 2 cut(s) 899, 1161
DpnI GATC 9 cut(s) 366, 442, 696, 884, 984, 1022, 1062, 1112, 1143
DpnII GATC 9 cut(s) 364, 440, 694, 882, 982, 1020, 1060, 1110, 1141
DraI TTTAAA 2 cut(s) 19, 1086
DrdI GACNNNNNNGTC 2 cut(s) 466, 599
DriI GACNNNNNGTC 2 cut(s) 499, 1156
DseDI GACNNNNNNGTC 2 cut(s) 466, 599
EaeI YGGCCR 1 cut(s) 624
Eam1104I CTCTTC 1 cut(s) 749
Eam1105I GACNNNNNGTC 2 cut(s) 499, 1156
EarI CTCTTC 1 cut(s) 749
EciI GGCGGA 3 cut(s) 382, 836, 922
Ecl136II GAGCTC 2 cut(s) 238, 930
Eco130I CCWWGG 1 cut(s) 1047
Eco147I AGGCCT 1 cut(s) 893
Eco24I GRGCYC 4 cut(s) 165, 240, 693, 932
Eco47III AGCGCT 1 cut(s) 799
Eco53kI GAGCTC 2 cut(s) 238, 930
EcoICRI GAGCTC 2 cut(s) 238, 930
EcoRI GAATTC 3 cut(s) 539, 856, 922
EcoRII CCWGG 4 cut(s) 44, 454, 661, 888
EcoT14I CCWWGG 1 cut(s) 1047
EcoT38I GRGCYC 4 cut(s) 165, 240, 693, 932
ErhI CCWWGG 1 cut(s) 1047
Esp3I CGTCTC 1 cut(s) 500
FaeI CATG 7 cut(s) 70, 465, 575, 631, 1027, 1074, 1106
FalI AAGNNNNNCTT 6 cut(s) 202, 234, 799, 831, 1050, 1082
FaqI GGGAC 1 cut(s) 472
FatI CATG 7 cut(s) 66, 461, 571, 627, 1023, 1070, 1102
FauI CCCGC 1 cut(s) 504
FbaI TGATCA 2 cut(s) 1020, 1110
FblI GTMKAC 1 cut(s) 603
Fnu4HI GCNGC 6 cut(s) 129, 179, 400, 403, 406, 828
FokI GGATG 1 cut(s) 282
FriOI GRGCYC 4 cut(s) 165, 240, 693, 932
Fsp4HI GCNGC 6 cut(s) 129, 179, 400, 403, 406, 828
FspBI CTAG 1 cut(s) 438
GlaI GCGC 3 cut(s) 252, 660, 799
GluI GCNGC 6 cut(s) 129, 179, 400, 403, 406, 828
GsuI CTGGAG 1 cut(s) 474
HaeII RGCGCY 1 cut(s) 801
HaeIII GGCC 2 cut(s) 626, 893
HapII CCGG 7 cut(s) 396, 544, 582, 687, 840, 877, 904
HgaI GACGC 2 cut(s) 606, 1032
HhaI GCGC 3 cut(s) 253, 661, 800
Hin1I GRCGYC 4 cut(s) 594, 606, 617, 1043
Hin1II CATG 7 cut(s) 70, 465, 575, 631, 1027, 1074, 1106
Hin6I GCGC 3 cut(s) 251, 659, 798
HinP1I GCGC 3 cut(s) 251, 659, 798
HincII GTYRAC 2 cut(s) 577, 604
HindII GTYRAC 2 cut(s) 577, 604
HinfI GANTC 4 cut(s) 425, 578, 677, 1004
HpaII CCGG 7 cut(s) 396, 544, 582, 687, 840, 877, 904
HphI GGTGA 3 cut(s) 232, 677, 751
Hpy166II GTNNAC 3 cut(s) 156, 577, 604
Hpy188I TCNGA 9 cut(s) 28, 364, 377, 505, 640, 705, 855, 952, 1093
Hpy188III TCNNGA 3 cut(s) 433, 491, 518
Hpy8I GTNNAC 3 cut(s) 156, 577, 604
Hpy99I CGWCG 7 cut(s) 524, 527, 530, 599, 608, 611, 922
HpyAV CCTTC 4 cut(s) 487, 801, 825, 860
HpyCH4III ACNGT 1 cut(s) 216
HpyCH4IV ACGT 2 cut(s) 594, 606
HpyCH4V TGCA 4 cut(s) 181, 827, 962, 1070
HpyF10VI GCNNNNNNNGC 6 cut(s) 169, 178, 184, 405, 623, 909
HpyF3I CTNAG 2 cut(s) 899, 1161
HpySE526I ACGT 2 cut(s) 594, 606
Hsp92I GRCGYC 4 cut(s) 594, 606, 617, 1043
Hsp92II CATG 7 cut(s) 70, 465, 575, 631, 1027, 1074, 1106
HspAI GCGC 3 cut(s) 251, 659, 798
KroI GCCGGC 2 cut(s) 395, 903
KroNI GCCGGC 2 cut(s) 397, 905
Ksp22I TGATCA 2 cut(s) 1020, 1110
Kzo9I GATC 9 cut(s) 364, 440, 694, 882, 982, 1020, 1060, 1110, 1141
LguI GCTCTTC 1 cut(s) 749
LmnI GCTCC 2 cut(s) 909, 942
Lsp1109I GCAGC 5 cut(s) 115, 165, 386, 392, 839
LweI GCATC 4 cut(s) 24, 260, 646, 954
MaeI CTAG 1 cut(s) 438
MaeII ACGT 2 cut(s) 594, 606
MaeIII GTNAC 1 cut(s) 618
MalI GATC 9 cut(s) 366, 442, 696, 884, 984, 1022, 1062, 1112, 1143
MboI GATC 9 cut(s) 364, 440, 694, 882, 982, 1020, 1060, 1110, 1141
MboII GAAGA 8 cut(s) 109, 126, 462, 763, 766, 781, 892, 1070
MfeI CAATTG 1 cut(s) 963
MflI RGATCY 2 cut(s) 440, 1060
MhlI GDGCHC 4 cut(s) 165, 240, 693, 932
MlsI TGGCCA 1 cut(s) 626
MluNI TGGCCA 1 cut(s) 626
MlyI GAGTC 1 cut(s) 572
MmeI TCCRAC 3 cut(s) 342, 897, 975
Mox20I TGGCCA 1 cut(s) 626
MroNI GCCGGC 2 cut(s) 395, 903
MscI TGGCCA 1 cut(s) 626
MseI TTAA 2 cut(s) 18, 1085
MslI CAYNNNNRTG 1 cut(s) 1118
Msp20I TGGCCA 1 cut(s) 626
MspI CCGG 7 cut(s) 396, 544, 582, 687, 840, 877, 904
MspR9I CCNGG 6 cut(s) 46, 456, 663, 688, 840, 890
MunI CAATTG 1 cut(s) 963
MvaI CCWGG 4 cut(s) 46, 456, 663, 890
MvnI CGCG 1 cut(s) 555
MwoI GCNNNNNNNGC 6 cut(s) 169, 178, 184, 405, 623, 909
NaeI GCCGGC 2 cut(s) 397, 905
NciI CCSGG 2 cut(s) 688, 840
NdeII GATC 9 cut(s) 364, 440, 694, 882, 982, 1020, 1060, 1110, 1141
NgoMIV GCCGGC 2 cut(s) 395, 903
NlaIII CATG 7 cut(s) 70, 465, 575, 631, 1027, 1074, 1106
NmeAIII GCCGAG 1 cut(s) 888
NmuCI GTSAC 1 cut(s) 618
NspI RCATGY 1 cut(s) 1074
PceI AGGCCT 1 cut(s) 893
PciSI GCTCTTC 1 cut(s) 749
PcsI WCGNNNNNNNCGW 4 cut(s) 525, 600, 603, 923
PdiI GCCGGC 2 cut(s) 397, 905
PfeI GAWTC 3 cut(s) 425, 677, 1004
PflFI GACNNNGTC 1 cut(s) 463
PflMI CCANNNNNTGG 1 cut(s) 45
PkrI GCNGC 6 cut(s) 130, 180, 401, 404, 407, 829
PleI GAGTC 1 cut(s) 572
PpsI GAGTC 1 cut(s) 572
PsiI TTATAA 1 cut(s) 710
Psp124BI GAGCTC 2 cut(s) 240, 932
Psp6I CCWGG 4 cut(s) 44, 454, 661, 888
PspGI CCWGG 4 cut(s) 44, 454, 661, 888
PsuI RGATCY 2 cut(s) 440, 1060
PsyI GACNNNGTC 1 cut(s) 463
RsaI GTAC 1 cut(s) 1100
RsaNI GTAC 1 cut(s) 1099
RseI CAYNNNNRTG 1 cut(s) 1118
SacI GAGCTC 2 cut(s) 240, 932
SalI GTCGAC 1 cut(s) 602
SapI GCTCTTC 1 cut(s) 749
SaqAI TTAA 2 cut(s) 18, 1085
SatI GCNGC 6 cut(s) 129, 179, 400, 403, 406, 828
Sau3AI GATC 9 cut(s) 364, 440, 694, 882, 982, 1020, 1060, 1110, 1141
SchI GAGTC 1 cut(s) 572
ScrFI CCNGG 6 cut(s) 46, 456, 663, 688, 840, 890
SduI GDGCHC 4 cut(s) 165, 240, 693, 932
SfaNI GCATC 4 cut(s) 24, 260, 646, 954
SmiMI CAYNNNNRTG 1 cut(s) 1118
SseBI AGGCCT 1 cut(s) 893
SsiI CCGC 8 cut(s) 393, 402, 497, 553, 564, 847, 907, 937
SspI AATATT 1 cut(s) 787
SspMI CTAG 1 cut(s) 438
SstI GAGCTC 2 cut(s) 240, 932
StuI AGGCCT 1 cut(s) 893
StyD4I CCNGG 6 cut(s) 44, 454, 661, 686, 838, 888
StyI CCWWGG 1 cut(s) 1047
TaaI ACNGT 1 cut(s) 216
TaiI ACGT 2 cut(s) 597, 609
TaqI TCGA 8 cut(s) 480, 519, 603, 609, 693, 920, 926, 932
TauI GCSGC 1 cut(s) 405
TfiI GAWTC 3 cut(s) 425, 677, 1004
Tru1I TTAA 2 cut(s) 18, 1085
Tru9I TTAA 2 cut(s) 18, 1085
TscAI CASTG 2 cut(s) 627, 1153
TseFI GTSAC 1 cut(s) 618
TseI GCWGC 5 cut(s) 128, 178, 399, 405, 827
Tsp45I GTSAC 1 cut(s) 618
TspDTI ATGAA 2 cut(s) 644, 807
TspGWI ACGGA 3 cut(s) 282, 418, 524
TspRI CASTG 2 cut(s) 627, 1153
Tth111I GACNNNGTC 1 cut(s) 463
Van91I CCANNNNNTGG 1 cut(s) 45
XapI RAATTY 5 cut(s) 539, 725, 856, 922, 1087
XceI RCATGY 1 cut(s) 1074
XcmI CCANNNNNNNNNTGG 2 cut(s) 452, 459
XmiI GTMKAC 1 cut(s) 603
XspI CTAG 1 cut(s) 438
ZraI GACGTC 2 cut(s) 595, 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.