pycom04g15240

DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0013954
Physical Location & Seq
Forward (+)
18131269 .. 18132667
1399 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g15240.1

Sequence Viewer

Length: 1332 bp
ATGAGTGCTGAAATTTTGATCACTATTTTCTCCTTGGTGGACCCGAACCCCCTTGCCAGAAAACTCAAGTCCTCTCTTCCTTCTTCCCTTCGTTTACACATCACTGAGTGGAAGAGAGAGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGTGGTGATGGGAAACCACAAGTTTAGATTATCAGATATGATGTCCAATTCCTGGTTTAGCAAGCTGAAAGACATGAGCAAGCCTAGAAAAAAGCAATCCAAGAAGAAAAAACAACAACAAAAGCCACCCTCGTTTGCTTCTTCTTCTCTAACAAAAAAATTTACAGAGCCCTCGAAGCCAACGCGGCAACTGCCCCACCAGCTCCACTGCCACCCAAGACAGTCTTACTACTTCACAAGAGAGCTCACCTCCCCTGCGCCTGCCCCATCTCACAGATTCCCCACCTCCTCTTCCCCCACCAATGACCCACCACCAAAAAAATCCAACAGACAAAAACTCAGGAACAGAATGACCACCTTGCCCTCTTCCGATCCCAACCTCCTAACTTCCTCTGTTTCAGCCGGCTGCGGCTGCCACGCCACCACCATTGAATCCGTCTGGACTAAATCCCAATCCCCACCTGAAACATGGTCGTCTTTCTCCTTCGACAGCTTCCCAGACCCGACGTCAGAGACGGAGTTTCTCGACGACGACAATGAGCCTGAATTCCGGTGCGACCGCGTTCTCGCCGCTTACCATGTCGACTCCGGTGAAAAAGACGTCCTTGTCGACGTCGACAAGGCGTCTCTGACCATGAAATTATCCGATGTGAAAGTATCGGATGCGCCGGAGGATGGGTTGGATTCATTTTCCGAGCTCGAGCTCCCTCCAATTATAACCAAGCCACCAAAATTCAGCGAAATCGTAAGCGAAGTGAAGAAGAACAAGAACAAGAAGAAAGAACAGAGGAGCATTACTAAAAGTGCTGGTAGAAGGCTTCCTTCATCAAATGCAGCTACATCTCCCGGCGTCCGCCTTCGGAATTCGCCGAGAATTTCCAACCGGAAGATCAACCAGGCCGGCCTCAGCCGGCGGAGCGTGTCGTCGAATTCGAGCTCGAAGCGAAGGAGCATCTCCGAAAGCTTCGCAATTGTCAAGTCATCGTTTGATCCCCAGAGGGATTTCAGGGAATCAATGGTGGAGATGATCATGGAGAACAACATCAAGGCGTCCAAGGATTTGGAAGATCTTCTTGCATGTTATCTTTCTTTGAATTCTGATGAGTACCATGAGATGATCATCAAGGTGTTCAAGCAAATCTGGATTGATCTCACTGATATTAGGTCCAAGTAA

Protein Analysis

444

Amino Acids

50.51

Weight (kDa)

9.58

Isoelectric Point (pI)

65.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_binding_2 PF13724 56 - 94 7.4e-16 DNA-binding domain
Ovate PF04844 381 - 437 3.5e-25 Transcriptional repressor, ovate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013954)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01840
fragaria_vesca FvH4_6g10140
malus_domestica MD04G1172500.v1.1 MD12G1185700.v1.1
prunus_persica Prupe.6G290900_v2.0.a1
pyrus_communis pycom04g15240 pycom12g17430
rosa_chinensis RchiOBHm_Chr3g0460841
rosa_laevigata RLG00000024975
rosa_multiflora Rmu_sc0004055.1_g000001
rosa_roxburghii Rroxscaffold_6G00419170
rosa_rugosa Rorug03G0046900.1
rosa_samantha Rh3AG104000 Rh3BG107600 Rh3CG109500 Rh3DG109500
rosa_wichuraiana Rw3G008850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 875
AasI GACNNNNNNGTC 1 cut(s) 764
AatII GACGTC 3 cut(s) 668, 762, 774
AccB7I CCANNNNNTGG 1 cut(s) 210
AccI GTMKAC 3 cut(s) 741, 768, 774
AccII CGCG 2 cut(s) 343, 720
AciI CCGC 6 cut(s) 343, 567, 718, 729, 1012, 1072
AclWI GGATC 2 cut(s) 524, 1142
AcsI RAATTY 8 cut(s) 12, 317, 704, 890, 1021, 1032, 1087, 1252
AcyI GRCGYC 6 cut(s) 665, 759, 771, 782, 1008, 1208
AdeI CACNNNGTG 1 cut(s) 108
AfaI GTAC 1 cut(s) 1265
AfiI CCNNNNNNNGG 2 cut(s) 210, 833
AgsI TTSAA 3 cut(s) 590, 1252, 1291
AhdI GACNNNNNGTC 2 cut(s) 664, 781
AjnI CCWGG 2 cut(s) 209, 1053
AluBI AGCT 9 cut(s) 223, 361, 403, 651, 856, 862, 995, 1095, 1122
AluI AGCT 9 cut(s) 223, 361, 403, 651, 856, 862, 995, 1095, 1122
Alw21I GWGCWC 4 cut(s) 405, 858, 864, 1097
Alw26I GTCTC 2 cut(s) 665, 789
AlwI GGATC 2 cut(s) 524, 1142
Ama87I CYCGRG 1 cut(s) 857
AoxI GGCC 2 cut(s) 1056, 1060
ApeKI GCWGC 3 cut(s) 564, 570, 992
ApoI RAATTY 8 cut(s) 12, 317, 704, 890, 1021, 1032, 1087, 1252
Asp700I GAANNNNTTC 1 cut(s) 1227
AspLEI GCGC 2 cut(s) 418, 826
AspS9I GGNCC 2 cut(s) 40, 1323
AsuC2I CCSGG 1 cut(s) 1005
AsuHPI GGTGA 3 cut(s) 175, 397, 761
AvaI CYCGRG 1 cut(s) 857
AvaII GGWCC 2 cut(s) 40, 1323
BanII GRGCYC 5 cut(s) 330, 405, 858, 864, 1097
Bbv12I GWGCWC 4 cut(s) 405, 858, 864, 1097
BbvCI CCTCAGC 1 cut(s) 1064
BbvI GCAGC 3 cut(s) 551, 557, 1004
BccI CCATC 3 cut(s) 160, 433, 827
BciT130I CCWGG 2 cut(s) 211, 1055
BclI TGATCA 3 cut(s) 18, 1185, 1275
BcnI CCSGG 1 cut(s) 1005
BcoDI GTCTC 2 cut(s) 665, 789
BfaI CTAG 1 cut(s) 243
BglI GCCNNNNNGGC 1 cut(s) 343
BglII AGATCT 1 cut(s) 1225
BisI GCNGC 6 cut(s) 344, 565, 568, 571, 729, 993
BlsI GCNGC 6 cut(s) 345, 566, 569, 572, 730, 994
Bme1390I CCNGG 3 cut(s) 211, 1005, 1055
Bme18I GGWCC 2 cut(s) 40, 1323
BmeRI GACNNNNNGTC 2 cut(s) 664, 781
BmeT110I CYCGRG 1 cut(s) 857
BmgT120I GGNCC 2 cut(s) 40, 1323
BmiI GGNNCC 1 cut(s) 42
BmrFI CCNGG 3 cut(s) 211, 1005, 1055
BmsI GCATC 2 cut(s) 811, 1119
BplI GAGNNNNNCTC 2 cut(s) 392, 424
Bpu10I CCTNAGC 1 cut(s) 1064
BpuEI CTTGAG 1 cut(s) 50
BpuMI CCSGG 1 cut(s) 1005
BsaBI GATNNNNATC 1 cut(s) 1277
BsaHI GRCGYC 6 cut(s) 665, 759, 771, 782, 1008, 1208
BsaJI CCNNGG 2 cut(s) 33, 1212
BsaWI WCCGGW 3 cut(s) 708, 746, 1041
BsaXI ACNNNNNCTCC 2 cut(s) 821, 851
Bsc4I CCNNNNNNNGG 2 cut(s) 210, 833
Bse118I RCCGGY 3 cut(s) 560, 1058, 1068
Bse8I GATNNNNATC 1 cut(s) 1277
BseBI CCWGG 2 cut(s) 211, 1055
BseDI CCNNGG 2 cut(s) 33, 1212
BseGI GGATG 2 cut(s) 826, 838
BseJI GATNNNNATC 1 cut(s) 1277
BseLI CCNNNNNNNGG 2 cut(s) 210, 833
BseMII CTCAG 3 cut(s) 96, 511, 1078
BseRI GAGGAG 3 cut(s) 134, 436, 961
BseXI GCAGC 3 cut(s) 551, 557, 1004
Bsh1236I CGCG 2 cut(s) 343, 720
Bsh1285I CGRYCG 1 cut(s) 718
BshFI GGCC 2 cut(s) 1058, 1062
BsiEI CGRYCG 1 cut(s) 718
BsiHKAI GWGCWC 4 cut(s) 405, 858, 864, 1097
BsiHKCI CYCGRG 1 cut(s) 857
BsiSI CCGG 8 cut(s) 561, 709, 747, 827, 1005, 1042, 1059, 1069
BslI CCNNNNNNNGG 2 cut(s) 210, 833
BsmAI GTCTC 2 cut(s) 665, 789
BsmBI CGTCTC 2 cut(s) 665, 789
BsnI GGCC 2 cut(s) 1058, 1062
BsoBI CYCGRG 1 cut(s) 857
Bsp1286I GDGCHC 5 cut(s) 330, 405, 858, 864, 1097
Bsp143I GATC 8 cut(s) 18, 529, 1047, 1147, 1185, 1225, 1275, 1306
BspACI CCGC 6 cut(s) 343, 567, 718, 729, 1012, 1072
BspANI GGCC 2 cut(s) 1058, 1062
BspCNI CTCAG 3 cut(s) 97, 510, 1077
BspFNI CGCG 2 cut(s) 343, 720
BspLI GGNNCC 1 cut(s) 42
BspPI GGATC 2 cut(s) 524, 1142
BsrFI RCCGGY 3 cut(s) 560, 1058, 1068
BssAI RCCGGY 3 cut(s) 560, 1058, 1068
BssECI CCNNGG 2 cut(s) 33, 1212
BssMI GATC 8 cut(s) 18, 529, 1047, 1147, 1185, 1225, 1275, 1306
BssNI GRCGYC 6 cut(s) 665, 759, 771, 782, 1008, 1208
BssT1I CCWWGG 2 cut(s) 33, 1212
Bst2UI CCWGG 2 cut(s) 211, 1055
Bst4CI ACNGT 1 cut(s) 381
Bst6I CTCTTC 4 cut(s) 81, 107, 454, 529
BstACI GRCGYC 6 cut(s) 665, 759, 771, 782, 1008, 1208
BstC8I GCNNGC 6 cut(s) 221, 239, 420, 562, 1060, 1070
BstDEI CTNAG 3 cut(s) 105, 497, 1064
BstF5I GGATG 2 cut(s) 826, 838
BstFNI CGCG 2 cut(s) 343, 720
BstHHI GCGC 2 cut(s) 418, 826
BstKTI GATC 8 cut(s) 21, 532, 1050, 1150, 1188, 1228, 1278, 1309
BstMAI GTCTC 2 cut(s) 665, 789
BstMBI GATC 8 cut(s) 18, 529, 1047, 1147, 1185, 1225, 1275, 1306
BstMCI CGRYCG 1 cut(s) 718
BstMWI GCNNNNNNNGC 6 cut(s) 334, 343, 349, 358, 570, 1074
BstNI CCWGG 2 cut(s) 211, 1055
BstNSI RCATGY 1 cut(s) 1239
BstSCI CCNGG 3 cut(s) 209, 1003, 1053
BstUI CGCG 2 cut(s) 343, 720
BstV1I GCAGC 3 cut(s) 551, 557, 1004
BstX2I RGATCY 1 cut(s) 1225
BstXI CCANNNNNNTGG 1 cut(s) 1219
BstYI RGATCY 1 cut(s) 1225
BsuRI GGCC 2 cut(s) 1058, 1062
BtsCI GGATG 2 cut(s) 826, 838
BtsI GCAGTG 1 cut(s) 364
BtsIMutI CAGTG 3 cut(s) 102, 364, 1311
Cac8I GCNNGC 6 cut(s) 221, 239, 420, 562, 1060, 1070
CfoI GCGC 2 cut(s) 418, 826
Cfr10I RCCGGY 3 cut(s) 560, 1058, 1068
Cfr13I GGNCC 2 cut(s) 40, 1323
CseI GACGC 3 cut(s) 771, 997, 1197
Csp6I GTAC 1 cut(s) 1264
CspCI CAANNNNNGTGG 2 cut(s) 568, 603
CviAII CATG 7 cut(s) 232, 627, 737, 793, 1189, 1236, 1268
CviQI GTAC 1 cut(s) 1264
DdeI CTNAG 3 cut(s) 105, 497, 1064
DpnI GATC 8 cut(s) 20, 531, 1049, 1149, 1187, 1227, 1277, 1308
DpnII GATC 8 cut(s) 18, 529, 1047, 1147, 1185, 1225, 1275, 1306
DraIII CACNNNGTG 1 cut(s) 108
DrdI GACNNNNNNGTC 1 cut(s) 764
DriI GACNNNNNGTC 2 cut(s) 664, 781
DseDI GACNNNNNNGTC 1 cut(s) 764
Eam1104I CTCTTC 4 cut(s) 81, 107, 454, 529
Eam1105I GACNNNNNGTC 2 cut(s) 664, 781
EarI CTCTTC 4 cut(s) 81, 107, 454, 529
EciI GGCGGA 2 cut(s) 1001, 1087
Ecl136II GAGCTC 4 cut(s) 403, 856, 862, 1095
Eco130I CCWWGG 2 cut(s) 33, 1212
Eco24I GRGCYC 5 cut(s) 330, 405, 858, 864, 1097
Eco47I GGWCC 2 cut(s) 40, 1323
Eco53kI GAGCTC 4 cut(s) 403, 856, 862, 1095
Eco88I CYCGRG 1 cut(s) 857
EcoICRI GAGCTC 4 cut(s) 403, 856, 862, 1095
EcoRI GAATTC 4 cut(s) 704, 1021, 1087, 1252
EcoRII CCWGG 2 cut(s) 209, 1053
EcoT14I CCWWGG 2 cut(s) 33, 1212
EcoT38I GRGCYC 5 cut(s) 330, 405, 858, 864, 1097
ErhI CCWWGG 2 cut(s) 33, 1212
Esp3I CGTCTC 2 cut(s) 665, 789
FaeI CATG 7 cut(s) 235, 630, 740, 796, 1192, 1239, 1271
FaiI YATR 9 cut(s) 197, 233, 628, 738, 794, 875, 1190, 1237, 1269
FalI AAGNNNNNCTT 8 cut(s) 367, 399, 747, 779, 964, 996, 1215, 1247
FatI CATG 7 cut(s) 231, 626, 736, 792, 1188, 1235, 1267
FbaI TGATCA 3 cut(s) 18, 1185, 1275
FblI GTMKAC 3 cut(s) 741, 768, 774
Fnu4HI GCNGC 6 cut(s) 344, 565, 568, 571, 729, 993
FokI GGATG 2 cut(s) 833, 845
FriOI GRGCYC 5 cut(s) 330, 405, 858, 864, 1097
FseI GGCCGGCC 1 cut(s) 1062
Fsp4HI GCNGC 6 cut(s) 344, 565, 568, 571, 729, 993
FspBI CTAG 1 cut(s) 243
GlaI GCGC 2 cut(s) 417, 825
GluI GCNGC 6 cut(s) 344, 565, 568, 571, 729, 993
HaeIII GGCC 2 cut(s) 1058, 1062
HapII CCGG 8 cut(s) 561, 709, 747, 827, 1005, 1042, 1059, 1069
HgaI GACGC 3 cut(s) 771, 997, 1197
HhaI GCGC 2 cut(s) 418, 826
Hin1I GRCGYC 6 cut(s) 665, 759, 771, 782, 1008, 1208
Hin1II CATG 7 cut(s) 235, 630, 740, 796, 1192, 1239, 1271
Hin6I GCGC 2 cut(s) 416, 824
HinP1I GCGC 2 cut(s) 416, 824
HincII GTYRAC 3 cut(s) 742, 769, 775
HindII GTYRAC 3 cut(s) 742, 769, 775
HindIII AAGCTT 1 cut(s) 1120
HinfI GANTC 5 cut(s) 435, 590, 743, 842, 1169
HpaII CCGG 8 cut(s) 561, 709, 747, 827, 1005, 1042, 1059, 1069
HphI GGTGA 3 cut(s) 175, 397, 761
Hpy166II GTNNAC 5 cut(s) 40, 95, 742, 769, 775
Hpy188III TCNNGA 4 cut(s) 499, 598, 683, 1300
Hpy8I GTNNAC 5 cut(s) 40, 95, 742, 769, 775
Hpy99I CGWCG 6 cut(s) 667, 689, 692, 773, 776, 1087
HpyAV CCTTC 7 cut(s) 90, 98, 652, 966, 990, 1025, 1098
HpyCH4III ACNGT 1 cut(s) 381
HpyCH4IV ACGT 3 cut(s) 665, 759, 771
HpyCH4V TGCA 2 cut(s) 992, 1235
HpyF10VI GCNNNNNNNGC 6 cut(s) 334, 343, 349, 358, 570, 1074
HpyF3I CTNAG 3 cut(s) 105, 497, 1064
HpySE526I ACGT 3 cut(s) 665, 759, 771
Hsp92I GRCGYC 6 cut(s) 665, 759, 771, 782, 1008, 1208
Hsp92II CATG 7 cut(s) 235, 630, 740, 796, 1192, 1239, 1271
HspAI GCGC 2 cut(s) 416, 824
KroI GCCGGC 3 cut(s) 560, 1058, 1068
KroNI GCCGGC 3 cut(s) 562, 1060, 1070
Ksp22I TGATCA 3 cut(s) 18, 1185, 1275
Kzo9I GATC 8 cut(s) 18, 529, 1047, 1147, 1185, 1225, 1275, 1306
LmnI GCTCC 5 cut(s) 366, 867, 948, 1074, 1107
Lsp1109I GCAGC 3 cut(s) 551, 557, 1004
LweI GCATC 2 cut(s) 811, 1119
MaeI CTAG 1 cut(s) 243
MaeII ACGT 3 cut(s) 665, 759, 771
MalI GATC 8 cut(s) 20, 531, 1049, 1149, 1187, 1227, 1277, 1308
MboI GATC 8 cut(s) 18, 529, 1047, 1147, 1185, 1225, 1275, 1306
MfeI CAATTG 1 cut(s) 1128
MflI RGATCY 1 cut(s) 1225
MhlI GDGCHC 5 cut(s) 330, 405, 858, 864, 1097
MlyI GAGTC 1 cut(s) 737
MmeI TCCRAC 3 cut(s) 507, 819, 1062
MroNI GCCGGC 3 cut(s) 560, 1058, 1068
MroXI GAANNNNTTC 1 cut(s) 1227
MslI CAYNNNNRTG 1 cut(s) 1283
MspI CCGG 8 cut(s) 561, 709, 747, 827, 1005, 1042, 1059, 1069
MspR9I CCNGG 3 cut(s) 211, 1005, 1055
MunI CAATTG 1 cut(s) 1128
MvaI CCWGG 2 cut(s) 211, 1055
MvnI CGCG 2 cut(s) 343, 720
MwoI GCNNNNNNNGC 6 cut(s) 334, 343, 349, 358, 570, 1074
NaeI GCCGGC 3 cut(s) 562, 1060, 1070
NciI CCSGG 1 cut(s) 1005
NdeII GATC 8 cut(s) 18, 529, 1047, 1147, 1185, 1225, 1275, 1306
NgoMIV GCCGGC 3 cut(s) 560, 1058, 1068
NlaIII CATG 7 cut(s) 235, 630, 740, 796, 1192, 1239, 1271
NlaIV GGNNCC 1 cut(s) 42
NmeAIII GCCGAG 1 cut(s) 1053
NspI RCATGY 1 cut(s) 1239
PaeR7I CTCGAG 1 cut(s) 857
PcsI WCGNNNNNNNCGW 2 cut(s) 765, 1088
PdiI GCCGGC 3 cut(s) 562, 1060, 1070
PdmI GAANNNNTTC 1 cut(s) 1227
PfeI GAWTC 4 cut(s) 435, 590, 842, 1169
PflMI CCANNNNNTGG 1 cut(s) 210
PkrI GCNGC 6 cut(s) 345, 566, 569, 572, 730, 994
PleI GAGTC 1 cut(s) 737
PpsI GAGTC 1 cut(s) 737
PsiI TTATAA 1 cut(s) 875
Psp124BI GAGCTC 4 cut(s) 405, 858, 864, 1097
Psp6I CCWGG 2 cut(s) 209, 1053
PspGI CCWGG 2 cut(s) 209, 1053
PspN4I GGNNCC 1 cut(s) 42
PspPI GGNCC 2 cut(s) 40, 1323
PspXI VCTCGAGB 1 cut(s) 857
PsuI RGATCY 1 cut(s) 1225
RigI GGCCGGCC 1 cut(s) 1062
RsaI GTAC 1 cut(s) 1265
RsaNI GTAC 1 cut(s) 1264
RseI CAYNNNNRTG 1 cut(s) 1283
SacI GAGCTC 4 cut(s) 405, 858, 864, 1097
SalI GTCGAC 3 cut(s) 740, 767, 773
SatI GCNGC 6 cut(s) 344, 565, 568, 571, 729, 993
Sau3AI GATC 8 cut(s) 18, 529, 1047, 1147, 1185, 1225, 1275, 1306
Sau96I GGNCC 2 cut(s) 40, 1323
SchI GAGTC 1 cut(s) 737
ScrFI CCNGG 3 cut(s) 211, 1005, 1055
SduI GDGCHC 5 cut(s) 330, 405, 858, 864, 1097
SfaNI GCATC 2 cut(s) 811, 1119
Sfr274I CTCGAG 1 cut(s) 857
SinI GGWCC 2 cut(s) 40, 1323
SlaI CTCGAG 1 cut(s) 857
SmiMI CAYNNNNRTG 1 cut(s) 1283
SmlI CTYRAG 2 cut(s) 65, 857
SmoI CTYRAG 2 cut(s) 65, 857
SsiI CCGC 6 cut(s) 343, 567, 718, 729, 1012, 1072
SspMI CTAG 1 cut(s) 243
SstI GAGCTC 4 cut(s) 405, 858, 864, 1097
StyD4I CCNGG 3 cut(s) 209, 1003, 1053
StyI CCWWGG 2 cut(s) 33, 1212
TaaI ACNGT 1 cut(s) 381
TaiI ACGT 3 cut(s) 668, 762, 774
TauI GCSGC 3 cut(s) 346, 570, 731
TfiI GAWTC 4 cut(s) 435, 590, 842, 1169
TscAI CASTG 3 cut(s) 109, 371, 1318
TseI GCWGC 3 cut(s) 564, 570, 992
TspDTI ATGAA 3 cut(s) 809, 834, 972
TspGWI ACGGA 2 cut(s) 583, 689
TspRI CASTG 3 cut(s) 109, 371, 1318
Van91I CCANNNNNTGG 1 cut(s) 210
VpaK11BI GGWCC 2 cut(s) 40, 1323
XapI RAATTY 8 cut(s) 12, 317, 704, 890, 1021, 1032, 1087, 1252
XceI RCATGY 1 cut(s) 1239
XcmI CCANNNNNNNNNTGG 1 cut(s) 624
XhoI CTCGAG 1 cut(s) 857
XmiI GTMKAC 3 cut(s) 741, 768, 774
XmnI GAANNNNTTC 1 cut(s) 1227
XspI CTAG 1 cut(s) 243
ZraI GACGTC 3 cut(s) 666, 760, 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.