Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0010210

Physical Location & Seq

Genomic Coordinates Reverse (-)

27325635 .. 27327881

Gene Structure 2247 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1190600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1422 bp

ATGGATGCCCACAAAAGAAAAATGGTGCCACAGAACATTATTTTGGTGACCATTTTTATGATTTTCATCAACCGTTGTAGTTCTACTTTCTTTGATGCCAGTGGAGTCGAGCAAATGGAAACTGTGGCGGAGGAAGCCGAAGATCATTTGGCGCATCCAAGCCCAAATGATTGGGTTGAAGAGGTGAATGGTGGAATGGAAGACGATGAGTGGCACTTAGTGCAGAAGAAAGGGAACCAATTTGTGGTGAATGATCAACCTTTCTATGTGAATGGATTCAACACTTACTGGCTGATGGTATTTGCTGCTGATCAATCCACAAGAGGAAAAGTCACTGATGTGTTTAAACAGGCATCTTCTGTCGGACTAACTGTTTGCAGGACTTGGGCTTTCAACGACGGCCAGTGGCAAGCTCTTCAGAAATCTCCATCAGTTTATGATGAAGAAGTTTTCAAGGCATTGGATTTTGTGATAAGTGAAGCAAGGAAATACAAGATCAGACTCATATTATCTTTAACCAACAACTGGGATGCATATGGTGGAAAAGCACAATATGTAAAATGGGGAAAAGCAGCTGGTGTCAATTTGACTTCTGATGACGACTTCTTCTCTAATCCAACACTCAAAAGCTACTACAAGGCCCATGTTAAGACGGTGCTTAATAGAGTTAATACACTCACAAACATAACTTACAAGGACGATCCCACAATTTTCTCTTGGGAACTGATGAATGAGCCTCGATGCACCTCAGATCCTTCGGGCGATAAACTGCAGGATTGGATACAAGAAATGGCAGTTTATGTTAAAAGCGTTGATCCAAAACACTTGGTAGAGATTGGATTGGAAGGATTTTATGGTCCCTCAGCACCTGACCGAGTTCAGTTCAATCCAAATCCATATGCTCAAGAAGTTGGAACTGATTTCATAAGGAACCACCAGGTTTTTGGTGTCGATTTTGCTTCTGTTCACATTTATGCAGACTCTTGGATATCATCGTCAATCTCTGATGTGCATCTCCAATTTACCAAGTCATGGATGGAAGCCCACATTGAGGATGCAGAGAAGTATCTTGGAATGCCGGTCTTGTTTTCAGAGTTTGGCGTATCTTCGAAAGACCCCAGCTACAATTCATCATTTAGAGACACCCTTCTCAGCACAGTGTACAAGACCATCCTCAATTCTAGTAAGAAAGGAGGGAGTGGAGGTGGGAGTCTTTTGTGGCAGCTCTTCCCAGAAGGAACGGACTACATGGATGACGGGTATGCAGTTGTTCTCTCAAAATCTCCTTCAACATCAAGCATCATAGCCCTTCACTCCACAAGACTTGCCCTATTCAACTCTCAATGTTCTTGGAAATGCCAGTGGGGTTGTAAGAAGAAGAATCCACTAGATGCATTCTTGTTACATCACGATGATCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0004567 GO:0005975 GO:0005976 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009791 GO:0009845 GO:0009987 GO:0010412 GO:0015923 GO:0016052 GO:0016787 GO:0016798 GO:0016985 GO:0016998 GO:0032501 GO:0032502 GO:0043170 GO:0044036 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0046355 GO:0048856 GO:0071554 GO:0071704 GO:0090351 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00051 map00051

Pfam Domains

Protein Families

Cellulase

Protein Analysis

474

Amino Acids

53.51

Weight (kDa)

5.27

Isoelectric Point (pI)

37.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GH5_mannosidase	PF26410	73 - 407	4.9e-50	GH family 5 beta-mannosidase-like
Cellulase	PF00150	85 - 373	5.2e-15	Cellulase (glycosyl hydrolase family 5)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010210)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01930
fragaria_vesca	FvH4_6g09620 FvH4_6g09620
malus_domestica	MD04G1176300.v1.1 MD12G1190600.v1.1
prunus_persica	Prupe.6G295800_v2.0.a1
pyrus_communis	pycom04g15550 pycom12g17780
rosa_chinensis	RchiOBHm_Chr3g0460261
rosa_laevigata	RLG00000025031
rosa_multiflora	Rmu_sc0000449.1_g000017 Rmu_sc0003763.1_g000002
rosa_roxburghii	Rroxscaffold_6G00419680
rosa_rugosa	Rorug03G0041200 Rorug03G0041200 Rorug03G0041200
rosa_samantha	Rh3AG099000 Rh3BG102800 Rh3CG104500 Rh3DG103800
rosa_wichuraiana	Rw3G008390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	25
AccB7I	CCANNNNNTGG	3 cut(s)	244, 525, 1032
AciI	CCGC	1 cut(s)	128
AclWI	GGATC	3 cut(s)	695, 746, 809
AcoI	YGGCCR	1 cut(s)	400
AcuI	CTGAAG	1 cut(s)	401
AdeI	CACNNNGTG	1 cut(s)	220
AfaI	GTAC	1 cut(s)	1163
AfiI	CCNNNNNNNGG	4 cut(s)	244, 525, 1032, 1051
AgsI	TTSAA	7 cut(s)	179, 280, 394, 454, 886, 1290, 1336
AjnI	CCWGG	1 cut(s)	936
AjuI	GAANNNNNNNTTGG	2 cut(s)	26, 58
AleI	CACNNNNGTG	1 cut(s)	338
AluBI	AGCT	5 cut(s)	413, 575, 630, 1122, 1225
AluI	AGCT	5 cut(s)	413, 575, 630, 1122, 1225
Alw26I	GTCTC	1 cut(s)	1134
AlwI	GGATC	3 cut(s)	695, 746, 809
AlwNI	CAGNNNCTG	1 cut(s)	869
AoxI	GGCC	2 cut(s)	400, 639
ApeKI	GCWGC	3 cut(s)	305, 572, 1222
ArsI	GACNNNNNNTTYG	2 cut(s)	357, 389
Asp700I	GAANNNNTTC	1 cut(s)	275
AspLEI	GCGC	1 cut(s)	154
AspS9I	GGNCC	2 cut(s)	640, 857
AsuHPI	GGTGA	3 cut(s)	58, 196, 259
AsuII	TTCGAA	1 cut(s)	1109
AvaII	GGWCC	1 cut(s)	857
BanI	GGYRCC	1 cut(s)	25
BbsI	GAAGAC	1 cut(s)	207
BbvCI	CCTCAGC	1 cut(s)	862
BbvI	GCAGC	3 cut(s)	292, 584, 1234
BccI	CCATC	4 cut(s)	289, 436, 1030, 1178
BceAI	ACGGC	1 cut(s)	415
BciT130I	CCWGG	1 cut(s)	938
BciVI	GTATCC	1 cut(s)	774
BclI	TGATCA	2 cut(s)	253, 310
BcoDI	GTCTC	1 cut(s)	1134
BfaI	CTAG	2 cut(s)	1182, 1388
BfmI	CTRYAG	1 cut(s)	770
BfuI	GTATCC	1 cut(s)	774
BisI	GCNGC	3 cut(s)	306, 573, 1223
BlsI	GCNGC	3 cut(s)	307, 574, 1224
Bme1390I	CCNGG	1 cut(s)	938
Bme18I	GGWCC	1 cut(s)	857
BmgT120I	GGNCC	2 cut(s)	640, 857
BmiI	GGNNCC	4 cut(s)	27, 236, 859, 932
BmrFI	CCNGG	1 cut(s)	938
BmrI	ACTGGG	1 cut(s)	535
BmsI	GCATC	9 cut(s)	85, 163, 362, 520, 731, 1021, 1045, 1308, 1381
BmuI	ACTGGG	1 cut(s)	535
BpiI	GAAGAC	1 cut(s)	207
Bpu10I	CCTNAGC	1 cut(s)	862
Bpu14I	TTCGAA	1 cut(s)	1109
BpuEI	CTTGAG	1 cut(s)	888
BsaBI	GATNNNNATC	2 cut(s)	65, 1011
BsaXI	ACNNNNNCTCC	2 cut(s)	1189, 1219
Bsc4I	CCNNNNNNNGG	4 cut(s)	244, 525, 1032, 1051
Bse118I	RCCGGY	1 cut(s)	1078
Bse1I	ACTGG	5 cut(s)	99, 293, 403, 530, 1360
Bse8I	GATNNNNATC	2 cut(s)	65, 1011
BseBI	CCWGG	1 cut(s)	938
BseGI	GGATG	7 cut(s)	10, 154, 535, 1041, 1060, 1170, 1258
BseJI	GATNNNNATC	2 cut(s)	65, 1011
BseLI	CCNNNNNNNGG	4 cut(s)	244, 525, 1032, 1051
BseMII	CTCAG	3 cut(s)	762, 876, 1165
BseNI	ACTGG	5 cut(s)	99, 293, 403, 530, 1360
BseXI	GCAGC	3 cut(s)	292, 584, 1234
BseYI	CCCAGC	1 cut(s)	1118
BsgI	GTGCAG	1 cut(s)	242
BshFI	GGCC	2 cut(s)	402, 641
BshNI	GGYRCC	1 cut(s)	25
BsiSI	CCGG	1 cut(s)	1079
BslFI	GGGAC	1 cut(s)	843
BslI	CCNNNNNNNGG	4 cut(s)	244, 525, 1032, 1051
BsmAI	GTCTC	1 cut(s)	1134
BsmFI	GGGAC	1 cut(s)	843
BsmI	GAATGC	2 cut(s)	1080, 1394
BsnI	GGCC	2 cut(s)	402, 641
Bsp119I	TTCGAA	1 cut(s)	1109
Bsp1407I	TGTACA	1 cut(s)	1161
Bsp143I	GATC	8 cut(s)	142, 253, 310, 495, 700, 751, 814, 1414
BspACI	CCGC	1 cut(s)	128
BspANI	GGCC	2 cut(s)	402, 641
BspCNI	CTCAG	3 cut(s)	761, 875, 1164
BspLI	GGNNCC	4 cut(s)	27, 236, 859, 932
BspMAI	CTGCAG	1 cut(s)	774
BspPI	GGATC	3 cut(s)	695, 746, 809
BspQI	GCTCTTC	2 cut(s)	420, 1232
BspT104I	TTCGAA	1 cut(s)	1109
BspT107I	GGYRCC	1 cut(s)	25
BsrFI	RCCGGY	1 cut(s)	1078
BsrGI	TGTACA	1 cut(s)	1161
BsrI	ACTGG	5 cut(s)	99, 293, 403, 530, 1360
BssAI	RCCGGY	1 cut(s)	1078
BssMI	GATC	8 cut(s)	142, 253, 310, 495, 700, 751, 814, 1414
Bst2UI	CCWGG	1 cut(s)	938
Bst4CI	ACNGT	5 cut(s)	74, 124, 373, 655, 1159
Bst6I	CTCTTC	3 cut(s)	174, 420, 1232
BstAPI	GCANNNNNTGC	1 cut(s)	220
BstAUI	TGTACA	1 cut(s)	1161
BstBI	TTCGAA	1 cut(s)	1109
BstC8I	GCNNGC	1 cut(s)	411
BstDEI	CTNAG	4 cut(s)	217, 748, 862, 1151
BstEII	GGTNACC	1 cut(s)	46
BstF5I	GGATG	7 cut(s)	10, 154, 535, 1041, 1060, 1170, 1258
BstHHI	GCGC	1 cut(s)	154
BstKTI	GATC	8 cut(s)	145, 256, 313, 498, 703, 754, 817, 1417
BstMAI	GTCTC	1 cut(s)	1134
BstMBI	GATC	8 cut(s)	142, 253, 310, 495, 700, 751, 814, 1414
BstMWI	GCNNNNNNNGC	2 cut(s)	134, 220
BstNI	CCWGG	1 cut(s)	938
BstPI	GGTNACC	1 cut(s)	46
BstSCI	CCNGG	1 cut(s)	936
BstSFI	CTRYAG	1 cut(s)	770
BstV1I	GCAGC	3 cut(s)	292, 584, 1234
BstV2I	GAAGAC	1 cut(s)	207
BstX2I	RGATCY	1 cut(s)	751
BstXI	CCANNNNNNTGG	2 cut(s)	171, 944
BstYI	RGATCY	1 cut(s)	751
BsuI	GTATCC	1 cut(s)	774
BsuRI	GGCC	2 cut(s)	402, 641
BtsCI	GGATG	7 cut(s)	10, 154, 535, 1041, 1060, 1170, 1258
BtsIMutI	CAGTG	5 cut(s)	106, 333, 410, 1164, 1367
Cac8I	GCNNGC	1 cut(s)	411
CaiI	CAGNNNCTG	1 cut(s)	869
CfoI	GCGC	1 cut(s)	154
Cfr10I	RCCGGY	1 cut(s)	1078
Cfr13I	GGNCC	2 cut(s)	640, 857
CsiI	ACCWGGT	1 cut(s)	936
Csp6I	GTAC	1 cut(s)	1162
CspCI	CAANNNNNGTGG	2 cut(s)	1306, 1341
CviAII	CATG	3 cut(s)	644, 1032, 1249
CviQI	GTAC	1 cut(s)	1162
DdeI	CTNAG	4 cut(s)	217, 748, 862, 1151
DpnI	GATC	8 cut(s)	144, 255, 312, 497, 702, 753, 816, 1416
DpnII	GATC	8 cut(s)	142, 253, 310, 495, 700, 751, 814, 1414
DraI	TTTAAA	1 cut(s)	346
DraIII	CACNNNGTG	1 cut(s)	220
EaeI	YGGCCR	1 cut(s)	400
Eam1104I	CTCTTC	3 cut(s)	174, 420, 1232
EarI	CTCTTC	3 cut(s)	174, 420, 1232
EciI	GGCGGA	1 cut(s)	143
Eco32I	GATATC	1 cut(s)	990
Eco47I	GGWCC	1 cut(s)	857
Eco57I	CTGAAG	1 cut(s)	401
Eco91I	GGTNACC	1 cut(s)	46
EcoO65I	GGTNACC	1 cut(s)	46
EcoRII	CCWGG	1 cut(s)	936
EcoRV	GATATC	1 cut(s)	990
EcoT22I	ATGCAT	2 cut(s)	535, 1396
FaeI	CATG	3 cut(s)	647, 1035, 1252
FaqI	GGGAC	1 cut(s)	843
FatI	CATG	3 cut(s)	643, 1031, 1248
FauNDI	CATATG	2 cut(s)	535, 898
FbaI	TGATCA	2 cut(s)	253, 310
Fnu4HI	GCNGC	3 cut(s)	306, 573, 1223
FokI	GGATG	7 cut(s)	17, 141, 542, 1048, 1067, 1157, 1265
Fsp4HI	GCNGC	3 cut(s)	306, 573, 1223
FspBI	CTAG	2 cut(s)	1182, 1388
GlaI	GCGC	1 cut(s)	153
GluI	GCNGC	3 cut(s)	306, 573, 1223
GsaI	CCCAGC	1 cut(s)	1122
HaeIII	GGCC	2 cut(s)	402, 641
HapII	CCGG	1 cut(s)	1079
HhaI	GCGC	1 cut(s)	154
Hin1II	CATG	3 cut(s)	647, 1035, 1252
Hin6I	GCGC	1 cut(s)	152
HinP1I	GCGC	1 cut(s)	152
HinfI	GANTC	6 cut(s)	105, 276, 501, 980, 1210, 1381
HpaII	CCGG	1 cut(s)	1079
HphI	GGTGA	3 cut(s)	58, 196, 259
Hpy166II	GTNNAC	2 cut(s)	967, 1162
Hpy188I	TCNGA	7 cut(s)	365, 420, 500, 595, 751, 1006, 1093
Hpy188III	TCNNGA	2 cut(s)	905, 1409
Hpy8I	GTNNAC	2 cut(s)	967, 1162
Hpy99I	CGWCG	1 cut(s)	401
HpyAV	CCTTC	6 cut(s)	765, 839, 1157, 1229, 1296, 1319
HpyCH4III	ACNGT	5 cut(s)	74, 124, 373, 655, 1159
HpyF10VI	GCNNNNNNNGC	2 cut(s)	134, 220
HpyF3I	CTNAG	4 cut(s)	217, 748, 862, 1151
Hsp92II	CATG	3 cut(s)	647, 1035, 1252
HspAI	GCGC	1 cut(s)	152
Ksp22I	TGATCA	2 cut(s)	253, 310
Kzo9I	GATC	8 cut(s)	142, 253, 310, 495, 700, 751, 814, 1414
LguI	GCTCTTC	2 cut(s)	420, 1232
Lsp1109I	GCAGC	3 cut(s)	292, 584, 1234
LweI	GCATC	9 cut(s)	85, 163, 362, 520, 731, 1021, 1045, 1308, 1381
MabI	ACCWGGT	1 cut(s)	936
MaeI	CTAG	2 cut(s)	1182, 1388
MaeIII	GTNAC	3 cut(s)	46, 331, 1401
MalI	GATC	8 cut(s)	144, 255, 312, 497, 702, 753, 816, 1416
MboI	GATC	8 cut(s)	142, 253, 310, 495, 700, 751, 814, 1414
MflI	RGATCY	1 cut(s)	751
MluCI	AATT	6 cut(s)	239, 583, 708, 1019, 1126, 1177
MlyI	GAGTC	4 cut(s)	114, 495, 974, 1219
MmeI	TCCRAC	3 cut(s)	343, 641, 892
Mph1103I	ATGCAT	2 cut(s)	535, 1396
MroXI	GAANNNNTTC	1 cut(s)	275
MseI	TTAA	6 cut(s)	345, 515, 648, 660, 669, 804
MslI	CAYNNNNRTG	4 cut(s)	56, 338, 972, 1410
MspA1I	CMGCKG	1 cut(s)	575
MspI	CCGG	1 cut(s)	1079
MspR9I	CCNGG	1 cut(s)	938
MssI	GTTTAAAC	1 cut(s)	346
Mva1269I	GAATGC	2 cut(s)	1080, 1394
MvaI	CCWGG	1 cut(s)	938
MwoI	GCNNNNNNNGC	2 cut(s)	134, 220
NdeI	CATATG	2 cut(s)	535, 898
NdeII	GATC	8 cut(s)	142, 253, 310, 495, 700, 751, 814, 1414
NlaIII	CATG	3 cut(s)	647, 1035, 1252
NlaIV	GGNNCC	4 cut(s)	27, 236, 859, 932
NmuCI	GTSAC	2 cut(s)	46, 331
NsiI	ATGCAT	2 cut(s)	535, 1396
NspV	TTCGAA	1 cut(s)	1109
OliI	CACNNNNGTG	1 cut(s)	338
PciSI	GCTCTTC	2 cut(s)	420, 1232
PctI	GAATGC	2 cut(s)	1080, 1394
PdmI	GAANNNNTTC	1 cut(s)	275
PfeI	GAWTC	2 cut(s)	276, 1381
PflMI	CCANNNNNTGG	3 cut(s)	244, 525, 1032
PkrI	GCNGC	3 cut(s)	307, 574, 1224
PleI	GAGTC	4 cut(s)	113, 495, 974, 1218
PmeI	GTTTAAAC	1 cut(s)	346
PpsI	GAGTC	4 cut(s)	113, 495, 974, 1218
Psp6I	CCWGG	1 cut(s)	936
PspEI	GGTNACC	1 cut(s)	46
PspFI	CCCAGC	1 cut(s)	1118
PspGI	CCWGG	1 cut(s)	936
PspN4I	GGNNCC	4 cut(s)	27, 236, 859, 932
PspPI	GGNCC	2 cut(s)	640, 857
PstI	CTGCAG	1 cut(s)	774
PstNI	CAGNNNCTG	1 cut(s)	869
PsuI	RGATCY	1 cut(s)	751
PvuII	CAGCTG	1 cut(s)	575
RsaI	GTAC	1 cut(s)	1163
RsaNI	GTAC	1 cut(s)	1162
RseI	CAYNNNNRTG	4 cut(s)	56, 338, 972, 1410
SapI	GCTCTTC	2 cut(s)	420, 1232
SaqAI	TTAA	6 cut(s)	345, 515, 648, 660, 669, 804
SatI	GCNGC	3 cut(s)	306, 573, 1223
Sau3AI	GATC	8 cut(s)	142, 253, 310, 495, 700, 751, 814, 1414
Sau96I	GGNCC	2 cut(s)	640, 857
SchI	GAGTC	4 cut(s)	114, 495, 974, 1219
ScrFI	CCNGG	1 cut(s)	938
SexAI	ACCWGGT	1 cut(s)	936
SfaNI	GCATC	9 cut(s)	85, 163, 362, 520, 731, 1021, 1045, 1308, 1381
SfcI	CTRYAG	1 cut(s)	770
SfuI	TTCGAA	1 cut(s)	1109
SinI	GGWCC	1 cut(s)	857
SmiMI	CAYNNNNRTG	4 cut(s)	56, 338, 972, 1410
SmlI	CTYRAG	1 cut(s)	903
SmoI	CTYRAG	1 cut(s)	903
Sse9I	AATT	6 cut(s)	239, 583, 708, 1019, 1126, 1177
SsiI	CCGC	1 cut(s)	128
SspMI	CTAG	2 cut(s)	1182, 1388
StyD4I	CCNGG	1 cut(s)	936
TaaI	ACNGT	5 cut(s)	74, 124, 373, 655, 1159
TaqI	TCGA	4 cut(s)	108, 739, 951, 1109
TaqII	GACCGA	1 cut(s)	888
TasI	AATT	6 cut(s)	239, 583, 708, 1019, 1126, 1177
TatI	WGTACW	1 cut(s)	1161
TfiI	GAWTC	2 cut(s)	276, 1381
Tru1I	TTAA	6 cut(s)	345, 515, 648, 660, 669, 804
Tru9I	TTAA	6 cut(s)	345, 515, 648, 660, 669, 804
TscAI	CASTG	5 cut(s)	106, 340, 410, 1164, 1367
TseFI	GTSAC	2 cut(s)	46, 331
TseI	GCWGC	3 cut(s)	305, 572, 1222
Tsp45I	GTSAC	2 cut(s)	46, 331
TspDTI	ATGAA	5 cut(s)	55, 456, 743, 913, 1119
TspGWI	ACGGA	1 cut(s)	1256
TspRI	CASTG	5 cut(s)	106, 340, 410, 1164, 1367
Van91I	CCANNNNNTGG	3 cut(s)	244, 525, 1032
VpaK11BI	GGWCC	1 cut(s)	857
XcmI	CCANNNNNNNNNTGG	2 cut(s)	941, 1033
XmnI	GAANNNNTTC	1 cut(s)	275
XspI	CTAG	2 cut(s)	1182, 1388
Zsp2I	ATGCAT	2 cut(s)	535, 1396

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.