MD12G1197000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012023
Physical Location & Seq
Forward (+)
27802757 .. 27803149
393 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1197000.v1.1.491

Sequence Viewer

Length: 393 bp
ATGGCCGACCTGTGGGGACAGAGCTTTCACCGCGCCGGAACCTATCCATCAATCAGCAGAAGCACCTCATTGGCTAGCAGTACTAGCAATGGATCCTACTACAACAGCTTCATGGAGCAGGGAAGCAGCAACTACACGTTGATGGAGAAGAGGCAGCTTTTTCTCAGAAGCTACCAGTTCAGCAGGAAGAAGAGCATGACGGAGAGGATAAAAGGGTCCTTTTACAAAGTCAAGAGGGTCATATGGCTGCGGCTGAGGTCGGCGAGGAAGCTCCGGAAGTTGGTTTGTTTCAGGCTCAGATATGGCGTTTCTTACCGGAGAAGAAGACTCTTCCGTCTGCTGAATAATTACCATCATGCCCGCAAATGCGATAACTCCTACTGTTTCTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

131

Amino Acids

15.73

Weight (kDa)

11.0

Isoelectric Point (pI)

60.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012023)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05937 AT5G26731
fragaria_vesca FvH4_6g08790
malus_domestica MD04G1183600.v1.1 MD12G1197000.v1.1
prunus_persica Prupe.6G302100_v2.0.a1
pyrus_communis pycom04g16210 pycom12g18330
rosa_chinensis RchiOBHm_Chr3g0459051
rosa_laevigata RLG00000025123
rosa_multiflora Rmu_sc0004343.1_g000014 Rmu_sc0006910.1_g000004 Rmu_sc0033812.1_g000005
rosa_roxburghii Rroxscaffold_6G00420550
rosa_rugosa Rorug03G0033200
rosa_samantha Rh3BG094100 Rh3CG094600 Rh3DG094600
rosa_wichuraiana Rw3G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 333
AccII CGCG 1 cut(s) 33
AccIII TCCGGA 1 cut(s) 273
AciI CCGC 3 cut(s) 31, 250, 361
AclWI GGATC 2 cut(s) 87, 100
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 1 cut(s) 82
AfiI CCNNNNNNNGG 2 cut(s) 12, 280
AflIII ACRYGT 1 cut(s) 135
AluBI AGCT 5 cut(s) 24, 108, 157, 171, 271
AluI AGCT 5 cut(s) 24, 108, 157, 171, 271
AlwI GGATC 2 cut(s) 87, 100
Aor13HI TCCGGA 1 cut(s) 273
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 3 cut(s) 126, 154, 247
AspLEI GCGC 1 cut(s) 35
AspS9I GGNCC 1 cut(s) 216
AsuHPI GGTGA 1 cut(s) 20
AsuNHI GCTAGC 1 cut(s) 74
AvaII GGWCC 1 cut(s) 216
BamHI GGATCC 1 cut(s) 92
BbsI GAAGAC 1 cut(s) 331
BbvCI CCTCAGC 1 cut(s) 254
BbvI GCAGC 3 cut(s) 138, 166, 234
BccI CCATC 3 cut(s) 55, 136, 360
BfaI CTAG 2 cut(s) 75, 84
BisI GCNGC 4 cut(s) 127, 155, 248, 251
BlsI GCNGC 4 cut(s) 128, 156, 249, 252
BmcAI AGTACT 1 cut(s) 82
Bme18I GGWCC 1 cut(s) 216
BmgT120I GGNCC 1 cut(s) 216
BmiI GGNNCC 3 cut(s) 40, 94, 217
BmtI GCTAGC 1 cut(s) 78
BpiI GAAGAC 1 cut(s) 331
Bpu10I CCTNAGC 1 cut(s) 254
BsaWI WCCGGW 2 cut(s) 273, 315
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 280
Bse1I ACTGG 1 cut(s) 175
Bse3DI GCAATG 1 cut(s) 94
BseAI TCCGGA 1 cut(s) 273
BseLI CCNNNNNNNGG 2 cut(s) 12, 280
BseMI GCAATG 1 cut(s) 94
BseMII CTCAG 3 cut(s) 178, 245, 310
BseNI ACTGG 1 cut(s) 175
BseXI GCAGC 3 cut(s) 138, 166, 234
Bsh1236I CGCG 1 cut(s) 33
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 3 cut(s) 36, 274, 316
BslFI GGGAC 1 cut(s) 30
BslI CCNNNNNNNGG 2 cut(s) 12, 280
BsmFI GGGAC 1 cut(s) 30
BsnI GGCC 1 cut(s) 5
Bsp13I TCCGGA 1 cut(s) 273
Bsp143I GATC 1 cut(s) 92
BspACI CCGC 3 cut(s) 31, 250, 361
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 3 cut(s) 177, 246, 309
BspEI TCCGGA 1 cut(s) 273
BspFNI CGCG 1 cut(s) 33
BspLI GGNNCC 3 cut(s) 40, 94, 217
BspOI GCTAGC 1 cut(s) 78
BspPI GGATC 2 cut(s) 87, 100
BspQI GCTCTTC 1 cut(s) 185
BsrDI GCAATG 1 cut(s) 94
BsrI ACTGG 1 cut(s) 175
BssMI GATC 1 cut(s) 92
Bst4CI ACNGT 1 cut(s) 383
Bst6I CTCTTC 3 cut(s) 143, 185, 335
BstC8I GCNNGC 2 cut(s) 76, 361
BstDEI CTNAG 3 cut(s) 164, 254, 296
BstFNI CGCG 1 cut(s) 33
BstHHI GCGC 1 cut(s) 35
BstKTI GATC 1 cut(s) 95
BstMBI GATC 1 cut(s) 92
BstMWI GCNNNNNNNGC 2 cut(s) 30, 84
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 3 cut(s) 138, 166, 234
BstV2I GAAGAC 1 cut(s) 331
BstX2I RGATCY 1 cut(s) 92
BstYI RGATCY 1 cut(s) 92
BsuRI GGCC 1 cut(s) 5
Cac8I GCNNGC 2 cut(s) 76, 361
CfoI GCGC 1 cut(s) 35
Cfr13I GGNCC 1 cut(s) 216
Csp6I GTAC 1 cut(s) 81
CviAII CATG 3 cut(s) 112, 196, 356
CviQI GTAC 1 cut(s) 81
DdeI CTNAG 3 cut(s) 164, 254, 296
DpnI GATC 1 cut(s) 94
DpnII GATC 1 cut(s) 92
DrdI GACNNNNNNGTC 1 cut(s) 333
DseDI GACNNNNNNGTC 1 cut(s) 333
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 143, 185, 335
EarI CTCTTC 3 cut(s) 143, 185, 335
Eco47I GGWCC 1 cut(s) 216
EcoO109I RGGNCCY 1 cut(s) 216
FaeI CATG 3 cut(s) 115, 199, 359
FaiI YATR 6 cut(s) 113, 197, 242, 244, 303, 357
FaqI GGGAC 1 cut(s) 30
FatI CATG 3 cut(s) 111, 195, 355
FauI CCCGC 1 cut(s) 368
FauNDI CATATG 1 cut(s) 242
Fnu4HI GCNGC 4 cut(s) 127, 155, 248, 251
Fsp4HI GCNGC 4 cut(s) 127, 155, 248, 251
FspBI CTAG 2 cut(s) 75, 84
GlaI GCGC 1 cut(s) 34
GluI GCNGC 4 cut(s) 127, 155, 248, 251
HaeIII GGCC 1 cut(s) 5
HapII CCGG 3 cut(s) 36, 274, 316
HhaI GCGC 1 cut(s) 35
Hin1II CATG 3 cut(s) 115, 199, 359
Hin6I GCGC 1 cut(s) 33
HinP1I GCGC 1 cut(s) 33
HinfI GANTC 1 cut(s) 327
HpaII CCGG 3 cut(s) 36, 274, 316
HphI GGTGA 1 cut(s) 20
Hpy188I TCNGA 2 cut(s) 167, 299
Hpy188III TCNNGA 2 cut(s) 232, 274
HpyCH4III ACNGT 1 cut(s) 383
HpyCH4IV ACGT 1 cut(s) 137
HpyF10VI GCNNNNNNNGC 2 cut(s) 30, 84
HpyF3I CTNAG 3 cut(s) 164, 254, 296
HpySE526I ACGT 1 cut(s) 137
Hsp92II CATG 3 cut(s) 115, 199, 359
HspAI GCGC 1 cut(s) 33
Kpn2I TCCGGA 1 cut(s) 273
Kzo9I GATC 1 cut(s) 92
LguI GCTCTTC 1 cut(s) 185
LmnI GCTCC 2 cut(s) 115, 276
LpnPI CCDG 9 cut(s) 23, 49, 104, 169, 188, 277, 287, 329, 373
Lsp1109I GCAGC 3 cut(s) 138, 166, 234
MaeI CTAG 2 cut(s) 75, 84
MaeII ACGT 1 cut(s) 137
MalI GATC 1 cut(s) 94
MboI GATC 1 cut(s) 92
MboII GAAGA 6 cut(s) 160, 199, 202, 322, 333, 336
MflI RGATCY 1 cut(s) 92
MluCI AATT 1 cut(s) 346
MlyI GAGTC 1 cut(s) 321
MnlI CCTC 6 cut(s) 76, 144, 198, 228, 249, 258
MroI TCCGGA 1 cut(s) 273
MslI CAYNNNNRTG 1 cut(s) 140
MspI CCGG 3 cut(s) 36, 274, 316
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 2 cut(s) 30, 84
NdeI CATATG 1 cut(s) 242
NdeII GATC 1 cut(s) 92
NheI GCTAGC 1 cut(s) 74
NlaIII CATG 3 cut(s) 115, 199, 359
NlaIV GGNNCC 3 cut(s) 40, 94, 217
PciSI GCTCTTC 1 cut(s) 185
PkrI GCNGC 4 cut(s) 128, 156, 249, 252
PleI GAGTC 1 cut(s) 321
PpsI GAGTC 1 cut(s) 321
PpuMI RGGWCCY 1 cut(s) 216
Psp5II RGGWCCY 1 cut(s) 216
PspN4I GGNNCC 3 cut(s) 40, 94, 217
PspPI GGNCC 1 cut(s) 216
PspPPI RGGWCCY 1 cut(s) 216
PsuI RGATCY 1 cut(s) 92
RsaI GTAC 1 cut(s) 82
RsaNI GTAC 1 cut(s) 81
RseI CAYNNNNRTG 1 cut(s) 140
SapI GCTCTTC 1 cut(s) 185
SatI GCNGC 4 cut(s) 127, 155, 248, 251
Sau3AI GATC 1 cut(s) 92
Sau96I GGNCC 1 cut(s) 216
ScaI AGTACT 1 cut(s) 82
SchI GAGTC 1 cut(s) 321
SinI GGWCC 1 cut(s) 216
SmiMI CAYNNNNRTG 1 cut(s) 140
Sse9I AATT 1 cut(s) 346
SsiI CCGC 3 cut(s) 31, 250, 361
SspMI CTAG 2 cut(s) 75, 84
TaaI ACNGT 1 cut(s) 383
TaiI ACGT 1 cut(s) 140
TasI AATT 1 cut(s) 346
TatI WGTACW 1 cut(s) 80
TauI GCSGC 1 cut(s) 253
TseI GCWGC 3 cut(s) 126, 154, 247
TspDTI ATGAA 1 cut(s) 100
TspGWI ACGGA 2 cut(s) 215, 323
VpaK11BI GGWCC 1 cut(s) 216
XspI CTAG 2 cut(s) 75, 84
ZrmI AGTACT 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.