RLG00000025123

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0012023
Physical Location & Seq
Forward (+)
42103436 .. 42104017
582 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025123

Sequence Viewer

Length: 465 bp
ATGGCCGGCCTGTGGGAGCCGCATCCGAACCCAACTCTCAGCAGAACCACTTCATCGAGTAGTACTAGTAGCTTTGGTGGGTTCTTCATGGATCATCAGAGCAATACTCACGGCGCTTATAACTACGTGATGATGGAGAAGATGGAGAAGAGGCAGCTTTTTCTTAGAAGCTACCAGTTCTGCAGGAAGAAGAGTCTGGCCGAGAAGATTAAAGGGTCTTTGGTTAAAGTGAAGAGGATCATATGCTTGAGGCTGCGTAATGCTAAAAAGCTTAGGAAATCTGTTTGCTTCAGATTCCGCCTTGGCAGTCTTCAAGGCCTGAGCCTTGGGATCAGCCTTCTTACCGTCAACTTTAGGGGCCATTGCTCAAACCTCTGCGTGAAACAATCCTACCTGAGGCGAAAGAGCAGCTGCGGCCGAAGAGTAGGGTTTCCACAACTCCATGTTAATTATTTGCTTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

155

Amino Acids

17.75

Weight (kDa)

10.65

Isoelectric Point (pI)

52.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012023)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05937 AT5G26731
fragaria_vesca FvH4_6g08790
malus_domestica MD04G1183600.v1.1 MD12G1197000.v1.1
prunus_persica Prupe.6G302100_v2.0.a1
pyrus_communis pycom04g16210 pycom12g18330
rosa_chinensis RchiOBHm_Chr3g0459051
rosa_laevigata RLG00000025123
rosa_multiflora Rmu_sc0004343.1_g000014 Rmu_sc0006910.1_g000004 Rmu_sc0033812.1_g000005
rosa_roxburghii Rroxscaffold_6G00420550
rosa_rugosa Rorug03G0033200
rosa_samantha Rh3BG094100 Rh3CG094600 Rh3DG094600
rosa_wichuraiana Rw3G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 120
AciI CCGC 3 cut(s) 20, 298, 414
AclWI GGATC 3 cut(s) 99, 245, 338
AcoI YGGCCR 3 cut(s) 3, 198, 415
AcuI CTGAAG 1 cut(s) 274
AfaI GTAC 1 cut(s) 64
AfiI CCNNNNNNNGG 2 cut(s) 12, 396
AgsI TTSAA 1 cut(s) 314
AhlI ACTAGT 1 cut(s) 65
AluBI AGCT 5 cut(s) 72, 157, 171, 271, 411
AluI AGCT 5 cut(s) 72, 157, 171, 271, 411
AlwI GGATC 3 cut(s) 99, 245, 338
AoxI GGCC 6 cut(s) 3, 7, 198, 316, 358, 415
ApeKI GCWGC 4 cut(s) 154, 253, 408, 411
Asp700I GAANNNNTTC 1 cut(s) 49
AspLEI GCGC 1 cut(s) 116
AspS9I GGNCC 1 cut(s) 358
AxyI CCTNAGG 1 cut(s) 395
BbsI GAAGAC 1 cut(s) 302
BbvI GCAGC 4 cut(s) 166, 240, 398, 420
BccI CCATC 2 cut(s) 127, 136
BceAI ACGGC 1 cut(s) 127
BcuI ACTAGT 1 cut(s) 65
BfaI CTAG 1 cut(s) 66
BfmI CTRYAG 1 cut(s) 181
BfoI RGCGCY 1 cut(s) 117
BisI GCNGC 6 cut(s) 20, 155, 254, 409, 412, 415
BlsI GCNGC 6 cut(s) 21, 156, 255, 410, 413, 416
BmcAI AGTACT 1 cut(s) 64
BmgT120I GGNCC 1 cut(s) 358
BmiI GGNNCC 2 cut(s) 18, 359
BmsI GCATC 1 cut(s) 31
BpiI GAAGAC 1 cut(s) 302
BplI GAGNNNNNCTC 2 cut(s) 91, 123
Bpu10I CCTNAGC 2 cut(s) 272, 320
BpuEI CTTGAG 1 cut(s) 268
BsaAI YACGTR 1 cut(s) 127
BsaJI CCNNGG 2 cut(s) 301, 325
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 396
Bse118I RCCGGY 1 cut(s) 5
Bse1I ACTGG 1 cut(s) 175
Bse21I CCTNAGG 1 cut(s) 395
Bse3DI GCAATG 1 cut(s) 361
BseDI CCNNGG 2 cut(s) 301, 325
BseGI GGATG 1 cut(s) 22
BseLI CCNNNNNNNGG 2 cut(s) 12, 396
BseMI GCAATG 1 cut(s) 361
BseMII CTCAG 3 cut(s) 52, 311, 386
BseNI ACTGG 1 cut(s) 175
BseX3I CGGCCG 1 cut(s) 415
BseXI GCAGC 4 cut(s) 166, 240, 398, 420
Bsh1285I CGRYCG 1 cut(s) 418
BshFI GGCC 6 cut(s) 5, 9, 200, 318, 360, 417
BsiEI CGRYCG 1 cut(s) 418
BsiSI CCGG 1 cut(s) 6
BslI CCNNNNNNNGG 2 cut(s) 12, 396
BsnI GGCC 6 cut(s) 5, 9, 200, 318, 360, 417
Bsp143I GATC 3 cut(s) 91, 237, 330
BspACI CCGC 3 cut(s) 20, 298, 414
BspANI GGCC 6 cut(s) 5, 9, 200, 318, 360, 417
BspCNI CTCAG 3 cut(s) 51, 312, 387
BspLI GGNNCC 2 cut(s) 18, 359
BspMAI CTGCAG 1 cut(s) 185
BspPI GGATC 3 cut(s) 99, 245, 338
BsrDI GCAATG 1 cut(s) 361
BsrFI RCCGGY 1 cut(s) 5
BsrI ACTGG 1 cut(s) 175
BssAI RCCGGY 1 cut(s) 5
BssECI CCNNGG 2 cut(s) 301, 325
BssMI GATC 3 cut(s) 91, 237, 330
BssT1I CCWWGG 2 cut(s) 301, 325
Bst4CI ACNGT 1 cut(s) 346
Bst6I CTCTTC 4 cut(s) 143, 185, 227, 415
BstBAI YACGTR 1 cut(s) 127
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 5 cut(s) 38, 164, 272, 320, 395
BstENI CCTNNNNNAGG 1 cut(s) 394
BstF5I GGATG 1 cut(s) 22
BstH2I RGCGCY 1 cut(s) 117
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 3 cut(s) 94, 240, 333
BstMBI GATC 3 cut(s) 91, 237, 330
BstMCI CGRYCG 1 cut(s) 418
BstMWI GCNNNNNNNGC 1 cut(s) 414
BstSFI CTRYAG 1 cut(s) 181
BstV1I GCAGC 4 cut(s) 166, 240, 398, 420
BstV2I GAAGAC 1 cut(s) 302
BstZI CGGCCG 1 cut(s) 415
Bsu36I CCTNAGG 1 cut(s) 395
BsuRI GGCC 6 cut(s) 5, 9, 200, 318, 360, 417
BtsCI GGATG 1 cut(s) 22
Cac8I GCNNGC 1 cut(s) 7
CfoI GCGC 1 cut(s) 116
Cfr10I RCCGGY 1 cut(s) 5
Cfr13I GGNCC 1 cut(s) 358
Csp6I GTAC 1 cut(s) 63
CviAII CATG 2 cut(s) 88, 443
CviQI GTAC 1 cut(s) 63
DdeI CTNAG 5 cut(s) 38, 164, 272, 320, 395
DpnI GATC 3 cut(s) 93, 239, 332
DpnII GATC 3 cut(s) 91, 237, 330
EaeI YGGCCR 3 cut(s) 3, 198, 415
EagI CGGCCG 1 cut(s) 415
Eam1104I CTCTTC 4 cut(s) 143, 185, 227, 415
EarI CTCTTC 4 cut(s) 143, 185, 227, 415
EciI GGCGGA 1 cut(s) 287
EclXI CGGCCG 1 cut(s) 415
Eco130I CCWWGG 2 cut(s) 301, 325
Eco147I AGGCCT 1 cut(s) 318
Eco52I CGGCCG 1 cut(s) 415
Eco57I CTGAAG 1 cut(s) 274
Eco81I CCTNAGG 1 cut(s) 395
EcoNI CCTNNNNNAGG 1 cut(s) 394
EcoT14I CCWWGG 2 cut(s) 301, 325
ErhI CCWWGG 2 cut(s) 301, 325
FaeI CATG 2 cut(s) 91, 446
FaiI YATR 5 cut(s) 89, 120, 242, 244, 444
FatI CATG 2 cut(s) 87, 442
FauNDI CATATG 1 cut(s) 242
Fnu4HI GCNGC 6 cut(s) 20, 155, 254, 409, 412, 415
FokI GGATG 1 cut(s) 9
FseI GGCCGGCC 1 cut(s) 9
Fsp4HI GCNGC 6 cut(s) 20, 155, 254, 409, 412, 415
FspBI CTAG 1 cut(s) 66
GlaI GCGC 1 cut(s) 115
GluI GCNGC 6 cut(s) 20, 155, 254, 409, 412, 415
HaeII RGCGCY 1 cut(s) 117
HaeIII GGCC 6 cut(s) 5, 9, 200, 318, 360, 417
HapII CCGG 1 cut(s) 6
HhaI GCGC 1 cut(s) 116
Hin1II CATG 2 cut(s) 91, 446
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HindIII AAGCTT 1 cut(s) 269
HinfI GANTC 2 cut(s) 193, 294
HpaII CCGG 1 cut(s) 6
Hpy166II GTNNAC 1 cut(s) 349
Hpy188I TCNGA 3 cut(s) 27, 99, 293
Hpy8I GTNNAC 1 cut(s) 349
HpyAV CCTTC 1 cut(s) 347
HpyCH4III ACNGT 1 cut(s) 346
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 1 cut(s) 183
HpyF10VI GCNNNNNNNGC 1 cut(s) 414
HpyF3I CTNAG 5 cut(s) 38, 164, 272, 320, 395
HpySE526I ACGT 1 cut(s) 126
Hsp92II CATG 2 cut(s) 91, 446
HspAI GCGC 1 cut(s) 114
KroI GCCGGC 1 cut(s) 5
KroNI GCCGGC 1 cut(s) 7
Kzo9I GATC 3 cut(s) 91, 237, 330
LmnI GCTCC 1 cut(s) 16
LpnPI CCDG 7 cut(s) 19, 23, 169, 182, 188, 332, 407
Lsp1109I GCAGC 4 cut(s) 166, 240, 398, 420
LweI GCATC 1 cut(s) 31
MaeI CTAG 1 cut(s) 66
MaeII ACGT 1 cut(s) 126
MalI GATC 3 cut(s) 93, 239, 332
MboI GATC 3 cut(s) 91, 237, 330
MboII GAAGA 9 cut(s) 76, 151, 160, 199, 202, 217, 244, 302, 432
MluCI AATT 1 cut(s) 448
MlyI GAGTC 1 cut(s) 202
MnlI CCTC 5 cut(s) 144, 228, 243, 383, 390
MroNI GCCGGC 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 49
MseI TTAA 3 cut(s) 210, 225, 447
MspA1I CMGCKG 1 cut(s) 411
MspI CCGG 1 cut(s) 6
MwoI GCNNNNNNNGC 1 cut(s) 414
NaeI GCCGGC 1 cut(s) 7
NdeI CATATG 1 cut(s) 242
NdeII GATC 3 cut(s) 91, 237, 330
NgoMIV GCCGGC 1 cut(s) 5
NlaIII CATG 2 cut(s) 91, 446
NlaIV GGNNCC 2 cut(s) 18, 359
NmeAIII GCCGAG 1 cut(s) 226
PceI AGGCCT 1 cut(s) 318
PdiI GCCGGC 1 cut(s) 7
PdmI GAANNNNTTC 1 cut(s) 49
PfeI GAWTC 1 cut(s) 294
PkrI GCNGC 6 cut(s) 21, 156, 255, 410, 413, 416
PleI GAGTC 1 cut(s) 201
PpsI GAGTC 1 cut(s) 201
Ppu21I YACGTR 1 cut(s) 127
PsiI TTATAA 1 cut(s) 120
PspN4I GGNNCC 2 cut(s) 18, 359
PspPI GGNCC 1 cut(s) 358
PstI CTGCAG 1 cut(s) 185
PvuII CAGCTG 1 cut(s) 411
RigI GGCCGGCC 1 cut(s) 9
RsaI GTAC 1 cut(s) 64
RsaNI GTAC 1 cut(s) 63
SaqAI TTAA 3 cut(s) 210, 225, 447
SatI GCNGC 6 cut(s) 20, 155, 254, 409, 412, 415
Sau3AI GATC 3 cut(s) 91, 237, 330
Sau96I GGNCC 1 cut(s) 358
ScaI AGTACT 1 cut(s) 64
SchI GAGTC 1 cut(s) 202
SetI ASST 8 cut(s) 74, 129, 159, 173, 273, 375, 396, 413
SfaNI GCATC 1 cut(s) 31
SfcI CTRYAG 1 cut(s) 181
SmlI CTYRAG 1 cut(s) 247
SmoI CTYRAG 1 cut(s) 247
SpeI ACTAGT 1 cut(s) 65
Sse9I AATT 1 cut(s) 448
SseBI AGGCCT 1 cut(s) 318
SsiI CCGC 3 cut(s) 20, 298, 414
SspMI CTAG 1 cut(s) 66
StuI AGGCCT 1 cut(s) 318
StyI CCWWGG 2 cut(s) 301, 325
TaaI ACNGT 1 cut(s) 346
TaiI ACGT 1 cut(s) 129
TaqI TCGA 1 cut(s) 56
TasI AATT 1 cut(s) 448
TatI WGTACW 1 cut(s) 62
TauI GCSGC 2 cut(s) 22, 417
TfiI GAWTC 1 cut(s) 294
Tru1I TTAA 3 cut(s) 210, 225, 447
Tru9I TTAA 3 cut(s) 210, 225, 447
TseI GCWGC 4 cut(s) 154, 253, 408, 411
TspDTI ATGAA 2 cut(s) 42, 76
XagI CCTNNNNNAGG 1 cut(s) 394
XmnI GAANNNNTTC 1 cut(s) 49
XspI CTAG 1 cut(s) 66
ZrmI AGTACT 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.