Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0005414

Physical Location & Seq

Genomic Coordinates Forward (+)

29418485 .. 29426130

Gene Structure 7646 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1216600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2841 bp

ATGGATCAACCACGTTTGGCCCTTGGGAATGGTGACGATAATGTCGGGATTCCGGATGATTTGCGATGCAAGAGATCAGATGGGAAACAGTGGAGGTGCACTGCCATGTCTATGCCAGATAAAACAGTATGTGAAAAACATTACATCCAGGCAAAGAAGAGGGCAGCTAATTCTGCAATGCGAGCAAATTTGAAAAAAGCCAAGAGGAAGTCGTTAGGTGAAAGTGATATTTACTTAGAGAGTAAGAGTGATGATTTGGATGTACCACTTGCTAGCGTGAAGTCACAGGAGAGAAAGTACATGGAGAAGGTATCAAAAAATCATTTTCGTTATTCACTTGAGAGACCACCCGTGAAGGGTTTATCTGTGCGTAATTCTCCAAAACCAAAGGACGAAATGGACTTGGATGAGTATGAGGAAAATTGGAGGTCTAATAAGTCACCACCTGCTAATGCTTTGGACTCATCCAGGAACAGACCGCAGAGGAGTTTTGATGTTAATGCTATGACAGTTTCGGAAGACTCTGATGGAAGATCAGAATCCTCTGAAGAAACTGGTGGCCAAACTTGCCATCAGTGTCGGAGGAATGACAGAGAGACAGTCATTTGGTGCCTCAAATGTGATAGAAGAGGATACTGTGACAGCTGTATTTCAACATGGTACTCGGACATCCCATTAGAAGAGATTCAGAGGTCCTGTCCAGCATGTCGTGGCACTTGTAATTGCAGAGTGTGCCTACGCAGAGATAATTTGGTAAAGGTAAGGATAAGAGAGATCCCAGTGCTAGATAAGTTGAAATATCTCCACTGTCTGCTGTCTTCAGTGCTTCCTATAGTTAAGCAAATCCATCAAGTACAATGCTTTGAAGTTGAACTGGAAAAAAAGCTTCGCGGAACTGATATAGATCTTGCCCGGACAAAATTGAATGCAGATGAGCAAATGTGCTGCAATTTCTGTAGAATTCCCATCATTGATTATCATTGGCACTGTTCCCGCTGCGCGTATGATCTCTGCCTTAACTGTTGTCGAGATCTTCGAGAAGCATCCATGCCTGGTGTTAGAGGAGAGGTGGTAGAGAATCAAATTGGTGAGGATAGGCGAGAAAAAGATACCAAGTTGGAGCAACCAAAATTGTCCAAAGTCAGAGTAAATTTAGCAGACAAGTTTTCCAACTGGAAAGCCCACAGTGATGGAAGTATCCCTTGCCCTCCAAAGGAGTATGGTGGCTGTGGCCATTCATCGTTGAATTTAAGCCGTATCTTTAAGATGAACTGGGTTGCAAAACTATTAAAAAATGCAGAGGAAATGGTTAGTGGCTGTACAGTCAATGAAGCTGTCAATCTGGAGAAAACTGGGCTTAACGATCCAAGACTTAGCCAGTATGCTCATAGAGAGGATAGCGATAATTTCTTGTACTGTGCTGCATCTGAAGATATCAAATCTGATGGGATTGGCAATTTTAAAAGGCATTGGCATAGGGGTGAGCCCATCATTGTTAAGGAGGTGTTTGATAGCTCATCAATTTCAAGCTGGGATCCAATGGTTATATGGAGAGGGATTCGGGAGACCGCAGATGAGAAATTAAAGGATGAGAACAGAAGGGTGAAGGCCATAAATTGCTGTGACTGGTCTGAGATTGATATTGAACTTAGTGAATTCATGAAAGGCTACTCCGAGGGAAGAGTTAATGAAAATGGTATGCCAGAAATGTTGAAACTCAAAGATTGGCCTTCTCCTAGTGCATCTGAAGAGTTCTTATTATATCAGAGACCTGAATTTATCAGCAGACTGCCCTTACTTGAGTATATTCACTCCAAGTTTGGTCTTTTAAATGTTGCTGCAAAACTGCCACACTATTCTTTGCAAAACGAGGTTGGACCTAAGATTTTTATTTCTTATGGGACCTATGAGGAACTTGGTAGACACGACTCCTTGACCAATCTCCATTTCAACATGCATGACATGGTATACCTGCTGGTGCACGCATGTGAGGTAAAGCTAAAAGGTTTGCAGAAGACAAAGATTGAGAACACACAAAAATCCGAGGAATCTGAGGTTGAAGAATCATCCGGGGATCTTCAAATGGGTATGGGAGAGGATACAAGGTCTGATGTATCACTTCTTGGTCAGAATGTGGAGAATGAATATGGGGCAACCTTGGCCTCAGATAAAGATGAGAGTACGGCAGACCATGGACATGAATCCACTCCTATGGTTGAAGATGACACCGCCAACTGTGAACAGTTAGAGAGAGACGGTAAAGATGTCTCTGAAAAAACTCATCTGGGAGTTATTTGGGATGTTTTCCGTCGACTGGATGTTCCAAAGCTGACTGAGTATTTGAGAATACATTGGCAAGAATTTGGGAAGCTCAATGAAACAAATATTTTTGTAACATTGCCTCTTTATGACGGGACATTGTTCCTGAATGCGGATCATAAAAGAAAATTGAAGGAGGAATTTGGAATTGAGCCATGGTCCTTTGAACAACATTTGGGGCAAGCTGTTTTTATTCCCGCTGGATGCCCTTTTCAAGTCAGGAGTCTTCAGTCAACTGTTCAGCTGGGTCTTGATTTCTTATCTCCTGAAAGCCTCGGTGAGGCTGCACGACTAGCTGATGAGATTCGCTGTCTTCCAAATGACCATGAAGCAAAGCTACAAGTGTTGGAGGTAGGAAAGATATCACTTTATGCGGCAAGTTCAGCCATTAAAGAGATTCAGAAGTTGGTACTCGATCCAAAACTTGGAGCAGAACTTGGATTTGAAGATCCAAATCTGACCGCGGCAGTTTCTGAGAATTTGGAGAAAATGACTAAACGAAGACAGATAACTTGTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008214 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031326 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0033169 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1903506 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03010 ko04714 map03010 map04714

Pfam Domains

Protein Families

JmjC WRC zf-4CXXC_R1

Protein Analysis

947

Amino Acids

107.82

Weight (kDa)

5.75

Isoelectric Point (pI)

48.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRC	PF08879	18 - 58	2.4e-14	WRC
zf-4CXXC_R1	PF10497	185 - 252	3e-08	Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC	PF02373	759 - 855	4.6e-07	JmjC domain, hydroxylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0005414)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09060 AT1G09060 AT1G09060 AT1G09060
fragaria_vesca	FvH4_6g06290 FvH4_6g06290 FvH4_6g06290 FvH4_6g06290
malus_domestica	MD04G1202800.v1.1 MD12G1216600.v1.1
prunus_persica	Prupe.6G322900_v2.0.a1 Prupe.6G322900_v2.0.a1 Prupe.6G322900_v2.0.a1 Prupe.6G322900_v2.0.a1 Prupe.6G322900_v2.0.a1
pyrus_communis	pycom04g17890 pycom12g20210
rosa_chinensis	RchiOBHm_Chr3g0455331
rosa_laevigata	RLG00000025369
rosa_multiflora	Rmu_sc0002169.1_g000043
rosa_roxburghii	Rroxscaffold_6G00424370
rosa_rugosa	Rorug03G0006300 Rorug03G0006400 Rorug03G0006500
rosa_samantha	Rh3AG066100 Rh3BG068300 Rh3CG066700 Rh3DG068200
rosa_wichuraiana	Rw3G005210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	454
AasI	GACNNNNNNGTC	1 cut(s)	41
Acc36I	ACCTGC	2 cut(s)	454, 1980
AccB1I	GGYRCC	1 cut(s)	609
AccB7I	CCANNNNNTGG	1 cut(s)	2744
AccI	GTMKAC	3 cut(s)	1921, 1968, 2311
AccII	CGCG	3 cut(s)	891, 1001, 2783
AccIII	TCCGGA	1 cut(s)	52
AclWI	GGATC	9 cut(s)	12, 769, 1358, 1529, 1542, 2082, 2442, 2729, 2762
AcoI	YGGCCR	2 cut(s)	559, 1231
AcsI	RAATTY	9 cut(s)	187, 960, 1150, 1246, 1655, 1775, 2360, 2459, 2797
AcuI	CTGAAG	5 cut(s)	567, 804, 1449, 1767, 2531
AfaI	GTAC	8 cut(s)	264, 299, 662, 855, 1321, 1415, 2182, 2730
AfiI	CCNNNNNNNGG	6 cut(s)	356, 1213, 2314, 2431, 2599, 2744
AjnI	CCWGG	3 cut(s)	147, 467, 1051
AjuI	GAANNNNNNNTTGG	2 cut(s)	1931, 1963
AleI	CACNNNNGTG	1 cut(s)	1986
Alw21I	GWGCWC	2 cut(s)	101, 1983
Alw26I	GTCTC	6 cut(s)	337, 590, 1559, 1762, 2247, 2272
Alw44I	GTGCAC	2 cut(s)	97, 1979
AlwI	GGATC	9 cut(s)	12, 769, 1358, 1529, 1542, 2082, 2442, 2729, 2762
AlwNI	CAGNNNCTG	1 cut(s)	2792
Aor13HI	TCCGGA	1 cut(s)	52
AoxI	GGCC	6 cut(s)	18, 559, 1231, 1608, 1727, 2160
ApaLI	GTGCAC	2 cut(s)	97, 1979
ApeKI	GCWGC	6 cut(s)	164, 945, 996, 1421, 1838, 2603
ApoI	RAATTY	9 cut(s)	187, 960, 1150, 1246, 1655, 1775, 2360, 2459, 2797
ArsI	GACNNNNNNTTYG	2 cut(s)	2323, 2355
Asp700I	GAANNNNTTC	1 cut(s)	684
AspLEI	GCGC	1 cut(s)	1001
AspS9I	GGNCC	5 cut(s)	19, 693, 1877, 1902, 2478
AsuC2I	CCSGG	2 cut(s)	913, 2071
AsuHPI	GGTGA	7 cut(s)	44, 230, 432, 1100, 1493, 1615, 2609
AsuNHI	GCTAGC	1 cut(s)	272
AvaII	GGWCC	4 cut(s)	693, 1877, 1902, 2478
BaeGI	GKGCMC	2 cut(s)	101, 1983
BalI	TGGCCA	2 cut(s)	561, 1233
BamHI	GGATCC	1 cut(s)	1534
BanI	GGYRCC	1 cut(s)	609
BanII	GRGCYC	1 cut(s)	1488
BarI	GAAGNNNNNNTAC	2 cut(s)	2640, 2672
BbsI	GAAGAC	6 cut(s)	525, 810, 2021, 2537, 2624, 2827
Bbv12I	GWGCWC	2 cut(s)	101, 1983
BbvI	GCAGC	6 cut(s)	176, 932, 983, 1408, 1825, 2590
BccI	CCATC	8 cut(s)	74, 521, 579, 855, 974, 1184, 1439, 1496
BceAI	ACGGC	2 cut(s)	1239, 2199
BciT130I	CCWGG	3 cut(s)	149, 469, 1053
BciVI	GTATCC	3 cut(s)	626, 1208, 2092
BcnI	CCSGG	2 cut(s)	913, 2071
BcoDI	GTCTC	6 cut(s)	337, 590, 1559, 1762, 2247, 2272
BfaI	CTAG	4 cut(s)	273, 785, 1737, 2612
BfmI	CTRYAG	2 cut(s)	831, 955
BfuAI	ACCTGC	2 cut(s)	454, 1980
BfuI	GTATCC	3 cut(s)	626, 1208, 2092
BglII	AGATCT	2 cut(s)	904, 1030
BisI	GCNGC	8 cut(s)	165, 946, 997, 1422, 1839, 2604, 2694, 2784
BlsI	GCNGC	8 cut(s)	166, 947, 998, 1423, 1840, 2605, 2695, 2785
Bme1390I	CCNGG	5 cut(s)	149, 469, 913, 1053, 2071
Bme18I	GGWCC	4 cut(s)	693, 1877, 1902, 2478
BmgT120I	GGNCC	5 cut(s)	19, 693, 1877, 1902, 2478
BmiI	GGNNCC	3 cut(s)	611, 1536, 1903
BmrFI	CCNGG	5 cut(s)	149, 469, 913, 1053, 2071
BmrI	ACTGGG	3 cut(s)	773, 1282, 1362
BmsI	GCATC	5 cut(s)	56, 1052, 1433, 1751, 2513
BmtI	GCTAGC	1 cut(s)	276
BmuI	ACTGGG	3 cut(s)	773, 1282, 1362
BpiI	GAAGAC	6 cut(s)	525, 810, 2021, 2537, 2624, 2827
BpmI	CTGGAG	1 cut(s)	1364
BpuEI	CTTGAG	2 cut(s)	359, 1820
BpuMI	CCSGG	2 cut(s)	913, 2071
BsaBI	GATNNNNATC	3 cut(s)	538, 903, 2772
BsaI	GGTCTC	3 cut(s)	337, 1559, 1762
BsaJI	CCNNGG	9 cut(s)	22, 1674, 2043, 2070, 2157, 2191, 2474, 2593, 2781
BsaWI	WCCGGW	1 cut(s)	52
BsaXI	ACNNNNNCTCC	4 cut(s)	281, 311, 1056, 1086
Bsc4I	CCNNNNNNNGG	6 cut(s)	356, 1213, 2314, 2431, 2599, 2744
Bse1I	ACTGG	9 cut(s)	559, 779, 879, 1178, 1277, 1357, 1378, 1631, 2319
Bse3DI	GCAATG	2 cut(s)	183, 2396
Bse8I	GATNNNNATC	3 cut(s)	538, 903, 2772
BseAI	TCCGGA	1 cut(s)	52
BseBI	CCWGG	3 cut(s)	149, 469, 1053
BseDI	CCNNGG	9 cut(s)	22, 1674, 2043, 2070, 2157, 2191, 2474, 2593, 2781
BseJI	GATNNNNATC	3 cut(s)	538, 903, 2772
BseLI	CCNNNNNNNGG	6 cut(s)	356, 1213, 2314, 2431, 2599, 2744
BseMI	GCAATG	2 cut(s)	183, 2396
BseMII	CTCAG	5 cut(s)	1623, 2043, 2178, 2325, 2784
BseNI	ACTGG	9 cut(s)	559, 779, 879, 1178, 1277, 1357, 1378, 1631, 2319
BseRI	GAGGAG	2 cut(s)	499, 1077
BseSI	GKGCMC	2 cut(s)	101, 1983
BseXI	GCAGC	6 cut(s)	176, 932, 983, 1408, 1825, 2590
BseYI	CCCAGC	2 cut(s)	1530, 2563
BsgI	GTGCAG	1 cut(s)	2589
Bsh1236I	CGCG	3 cut(s)	891, 1001, 2783
BshFI	GGCC	6 cut(s)	20, 561, 1233, 1610, 1729, 2162
BshNI	GGYRCC	1 cut(s)	609
BsiHKAI	GWGCWC	2 cut(s)	101, 1983
BsiSI	CCGG	3 cut(s)	53, 913, 2070
BslFI	GGGAC	2 cut(s)	1915, 2428
BslI	CCNNNNNNNGG	6 cut(s)	356, 1213, 2314, 2431, 2599, 2744
BsmAI	GTCTC	6 cut(s)	337, 590, 1559, 1762, 2247, 2272
BsmBI	CGTCTC	1 cut(s)	2247
BsmFI	GGGAC	2 cut(s)	1915, 2428
BsmI	GAATGC	2 cut(s)	931, 2434
BsnI	GGCC	6 cut(s)	20, 561, 1233, 1610, 1729, 2162
Bso31I	GGTCTC	3 cut(s)	337, 1559, 1762
Bsp1286I	GDGCHC	3 cut(s)	101, 1488, 1983
Bsp13I	TCCGGA	1 cut(s)	52
Bsp1407I	TGTACA	1 cut(s)	1319
Bsp19I	CCATGG	2 cut(s)	2191, 2474
BspANI	GGCC	6 cut(s)	20, 561, 1233, 1610, 1729, 2162
BspCNI	CTCAG	5 cut(s)	1624, 2044, 2177, 2326, 2785
BspEI	TCCGGA	1 cut(s)	52
BspFNI	CGCG	3 cut(s)	891, 1001, 2783
BspHI	TCATGA	1 cut(s)	1659
BspLI	GGNNCC	3 cut(s)	611, 1536, 1903
BspMI	ACCTGC	2 cut(s)	454, 1980
BspOI	GCTAGC	1 cut(s)	276
BspPI	GGATC	9 cut(s)	12, 769, 1358, 1529, 1542, 2082, 2442, 2729, 2762
BspT107I	GGYRCC	1 cut(s)	609
BspTNI	GGTCTC	3 cut(s)	337, 1559, 1762
BsrDI	GCAATG	2 cut(s)	183, 2396
BsrGI	TGTACA	1 cut(s)	1319
BsrI	ACTGG	9 cut(s)	559, 779, 879, 1178, 1277, 1357, 1378, 1631, 2319
BssECI	CCNNGG	9 cut(s)	22, 1674, 2043, 2070, 2157, 2191, 2474, 2593, 2781
BssNAI	GTATAC	1 cut(s)	1969
BssT1I	CCWWGG	4 cut(s)	22, 2157, 2191, 2474
Bst1107I	GTATAC	1 cut(s)	1969
Bst2UI	CCWGG	3 cut(s)	149, 469, 1053
Bst6I	CTCTTC	5 cut(s)	152, 622, 675, 1675, 1743
BstAPI	GCANNNNNTGC	1 cut(s)	732
BstAUI	TGTACA	1 cut(s)	1319
BstC8I	GCNNGC	4 cut(s)	183, 274, 1983, 2502
BstDEI	CTNAG	9 cut(s)	235, 1373, 1632, 1649, 1881, 2052, 2164, 2334, 2793
BstDSI	CCRYGG	3 cut(s)	2191, 2474, 2781
BstENI	CCTNNNNNAGG	1 cut(s)	2597
BstFNI	CGCG	3 cut(s)	891, 1001, 2783
BstHHI	GCGC	1 cut(s)	1001
BstMAI	GTCTC	6 cut(s)	337, 590, 1559, 1762, 2247, 2272
BstMWI	GCNNNNNNNGC	7 cut(s)	173, 182, 567, 732, 2159, 2612, 2702
BstNI	CCWGG	3 cut(s)	149, 469, 1053
BstNSI	RCATGY	3 cut(s)	708, 1957, 1989
BstSCI	CCNGG	5 cut(s)	147, 467, 911, 1051, 2069
BstSFI	CTRYAG	2 cut(s)	831, 955
BstSLI	GKGCMC	2 cut(s)	101, 1983
BstUI	CGCG	3 cut(s)	891, 1001, 2783
BstV1I	GCAGC	6 cut(s)	176, 932, 983, 1408, 1825, 2590
BstV2I	GAAGAC	6 cut(s)	525, 810, 2021, 2537, 2624, 2827
BstX2I	RGATCY	6 cut(s)	774, 904, 1030, 1534, 2074, 2767
BstXI	CCANNNNNNTGG	2 cut(s)	1190, 2212
BstYI	RGATCY	6 cut(s)	774, 904, 1030, 1534, 2074, 2767
BstZ17I	GTATAC	1 cut(s)	1969
BsuI	GTATCC	3 cut(s)	626, 1208, 2092
BsuRI	GGCC	6 cut(s)	20, 561, 1233, 1610, 1729, 2162
BtgI	CCRYGG	3 cut(s)	2191, 2474, 2781
BtgZI	GCGATG	1 cut(s)	79
BtsI	GCAGTG	1 cut(s)	99
BtsIMutI	CAGTG	8 cut(s)	95, 99, 581, 786, 805, 828, 985, 1192
BveI	ACCTGC	2 cut(s)	454, 1980
Cac8I	GCNNGC	4 cut(s)	183, 274, 1983, 2502
CaiI	CAGNNNCTG	1 cut(s)	2792
CciI	TCATGA	1 cut(s)	1659
CfoI	GCGC	1 cut(s)	1001
Cfr13I	GGNCC	5 cut(s)	19, 693, 1877, 1902, 2478
Cfr42I	CCGCGG	1 cut(s)	2784
Csp6I	GTAC	8 cut(s)	263, 298, 661, 854, 1320, 1414, 2181, 2729
CviQI	GTAC	8 cut(s)	263, 298, 661, 854, 1320, 1414, 2181, 2729
DdeI	CTNAG	9 cut(s)	235, 1373, 1632, 1649, 1881, 2052, 2164, 2334, 2793
DraI	TTTAAA	2 cut(s)	1462, 1830
DrdI	GACNNNNNNGTC	1 cut(s)	41
DseDI	GACNNNNNNGTC	1 cut(s)	41
EaeI	YGGCCR	2 cut(s)	559, 1231
Eam1104I	CTCTTC	5 cut(s)	152, 622, 675, 1675, 1743
EarI	CTCTTC	5 cut(s)	152, 622, 675, 1675, 1743
Eco130I	CCWWGG	4 cut(s)	22, 2157, 2191, 2474
Eco24I	GRGCYC	1 cut(s)	1488
Eco31I	GGTCTC	3 cut(s)	337, 1559, 1762
Eco32I	GATATC	2 cut(s)	1435, 2682
Eco47I	GGWCC	4 cut(s)	693, 1877, 1902, 2478
Eco57I	CTGAAG	5 cut(s)	567, 804, 1449, 1767, 2531
EcoNI	CCTNNNNNAGG	1 cut(s)	2597
EcoO109I	RGGNCCY	2 cut(s)	693, 1902
EcoRI	GAATTC	2 cut(s)	960, 1655
EcoRII	CCWGG	3 cut(s)	147, 467, 1051
EcoRV	GATATC	2 cut(s)	1435, 2682
EcoT14I	CCWWGG	4 cut(s)	22, 2157, 2191, 2474
EcoT22I	ATGCAT	1 cut(s)	1959
EcoT38I	GRGCYC	1 cut(s)	1488
ErhI	CCWWGG	4 cut(s)	22, 2157, 2191, 2474
Esp3I	CGTCTC	1 cut(s)	2247
FalI	AAGNNNNNCTT	2 cut(s)	1186, 1218
FaqI	GGGAC	2 cut(s)	1915, 2428
FauI	CCCGC	2 cut(s)	1001, 2524
FblI	GTMKAC	3 cut(s)	1921, 1968, 2311
Fnu4HI	GCNGC	8 cut(s)	165, 946, 997, 1422, 1839, 2604, 2694, 2784
FriOI	GRGCYC	1 cut(s)	1488
Fsp4HI	GCNGC	8 cut(s)	165, 946, 997, 1422, 1839, 2604, 2694, 2784
FspBI	CTAG	4 cut(s)	273, 785, 1737, 2612
GlaI	GCGC	1 cut(s)	1000
GluI	GCNGC	8 cut(s)	165, 946, 997, 1422, 1839, 2604, 2694, 2784
GsaI	CCCAGC	2 cut(s)	1534, 2567
GsuI	CTGGAG	1 cut(s)	1364
HaeIII	GGCC	6 cut(s)	20, 561, 1233, 1610, 1729, 2162
HapII	CCGG	3 cut(s)	53, 913, 2070
HhaI	GCGC	1 cut(s)	1001
Hin6I	GCGC	1 cut(s)	999
HinP1I	GCGC	1 cut(s)	999
HincII	GTYRAC	2 cut(s)	2312, 2553
HindII	GTYRAC	2 cut(s)	2312, 2553
HindIII	AAGCTT	1 cut(s)	884
HpaII	CCGG	3 cut(s)	53, 913, 2070
HphI	GGTGA	7 cut(s)	44, 230, 432, 1100, 1493, 1615, 2609
Hpy166II	GTNNAC	7 cut(s)	99, 1922, 1969, 1981, 2240, 2312, 2553
Hpy8I	GTNNAC	7 cut(s)	99, 1922, 1969, 1981, 2240, 2312, 2553
Hpy99I	CGWCG	1 cut(s)	2313
HpyAV	CCTTC	6 cut(s)	301, 349, 1593, 1600, 1740, 2446
HpyCH4IV	ACGT	1 cut(s)	13
HpyF10VI	GCNNNNNNNGC	7 cut(s)	173, 182, 567, 732, 2159, 2612, 2702
HpyF3I	CTNAG	9 cut(s)	235, 1373, 1632, 1649, 1881, 2052, 2164, 2334, 2793
HpySE526I	ACGT	1 cut(s)	13
HspAI	GCGC	1 cut(s)	999
Kpn2I	TCCGGA	1 cut(s)	52
KspI	CCGCGG	1 cut(s)	2784
LmnI	GCTCC	2 cut(s)	1120, 2747
Lsp1109I	GCAGC	6 cut(s)	176, 932, 983, 1408, 1825, 2590
LweI	GCATC	5 cut(s)	56, 1052, 1433, 1751, 2513
MaeI	CTAG	4 cut(s)	273, 785, 1737, 2612
MaeII	ACGT	1 cut(s)	13
MaeIII	GTNAC	6 cut(s)	32, 282, 438, 638, 1622, 2392
MflI	RGATCY	6 cut(s)	774, 904, 1030, 1534, 2074, 2767
MhlI	GDGCHC	3 cut(s)	101, 1488, 1983
MlsI	TGGCCA	2 cut(s)	561, 1233
MluNI	TGGCCA	2 cut(s)	561, 1233
MlyI	GAGTC	4 cut(s)	455, 515, 1922, 2551
MmeI	TCCRAC	5 cut(s)	560, 1098, 1194, 1855, 2646
Mox20I	TGGCCA	2 cut(s)	561, 1233
Mph1103I	ATGCAT	1 cut(s)	1959
MroI	TCCGGA	1 cut(s)	52
MroXI	GAANNNNTTC	1 cut(s)	684
MscI	TGGCCA	2 cut(s)	561, 1233
MslI	CAYNNNNRTG	7 cut(s)	104, 110, 1188, 1479, 1986, 2196, 2210
Msp20I	TGGCCA	2 cut(s)	561, 1233
MspA1I	CMGCKG	5 cut(s)	645, 996, 2519, 2563, 2783
MspI	CCGG	3 cut(s)	53, 913, 2070
MspR9I	CCNGG	5 cut(s)	149, 469, 913, 1053, 2071
Mva1269I	GAATGC	2 cut(s)	931, 2434
MvaI	CCWGG	3 cut(s)	149, 469, 1053
MvnI	CGCG	3 cut(s)	891, 1001, 2783
MwoI	GCNNNNNNNGC	7 cut(s)	173, 182, 567, 732, 2159, 2612, 2702
NciI	CCSGG	2 cut(s)	913, 2071
NcoI	CCATGG	2 cut(s)	2191, 2474
NheI	GCTAGC	1 cut(s)	272
NlaIV	GGNNCC	3 cut(s)	611, 1536, 1903
NmuCI	GTSAC	5 cut(s)	32, 282, 438, 638, 1622
NsiI	ATGCAT	1 cut(s)	1959
NspI	RCATGY	3 cut(s)	708, 1957, 1989
OliI	CACNNNNGTG	1 cut(s)	1986
PagI	TCATGA	1 cut(s)	1659
PaqCI	CACCTGC	1 cut(s)	454
PcsI	WCGNNNNNNNCGW	1 cut(s)	1033
PctI	GAATGC	2 cut(s)	931, 2434
PdmI	GAANNNNTTC	1 cut(s)	684
PflMI	CCANNNNNTGG	1 cut(s)	2744
PfoI	TCCNGGA	1 cut(s)	467
PkrI	GCNGC	8 cut(s)	166, 947, 998, 1423, 1840, 2605, 2695, 2785
PleI	GAGTC	4 cut(s)	455, 515, 1922, 2550
PpsI	GAGTC	4 cut(s)	455, 515, 1922, 2550
PpuMI	RGGWCCY	2 cut(s)	693, 1902
Psp5II	RGGWCCY	2 cut(s)	693, 1902
Psp6I	CCWGG	3 cut(s)	147, 467, 1051
PspFI	CCCAGC	2 cut(s)	1530, 2563
PspGI	CCWGG	3 cut(s)	147, 467, 1051
PspN4I	GGNNCC	3 cut(s)	611, 1536, 1903
PspPI	GGNCC	5 cut(s)	19, 693, 1877, 1902, 2478
PspPPI	RGGWCCY	2 cut(s)	693, 1902
PstNI	CAGNNNCTG	1 cut(s)	2792
PsuI	RGATCY	6 cut(s)	774, 904, 1030, 1534, 2074, 2767
PvuII	CAGCTG	2 cut(s)	645, 2563
RsaI	GTAC	8 cut(s)	264, 299, 662, 855, 1321, 1415, 2182, 2730
RsaNI	GTAC	8 cut(s)	263, 298, 661, 854, 1320, 1414, 2181, 2729
RseI	CAYNNNNRTG	7 cut(s)	104, 110, 1188, 1479, 1986, 2196, 2210
SacII	CCGCGG	1 cut(s)	2784
SalI	GTCGAC	1 cut(s)	2310
SatI	GCNGC	8 cut(s)	165, 946, 997, 1422, 1839, 2604, 2694, 2784
Sau96I	GGNCC	5 cut(s)	19, 693, 1877, 1902, 2478
SchI	GAGTC	4 cut(s)	455, 515, 1922, 2551
ScrFI	CCNGG	5 cut(s)	149, 469, 913, 1053, 2071
SduI	GDGCHC	3 cut(s)	101, 1488, 1983
SfaNI	GCATC	5 cut(s)	56, 1052, 1433, 1751, 2513
SfcI	CTRYAG	2 cut(s)	831, 955
Sfr303I	CCGCGG	1 cut(s)	2784
SgrBI	CCGCGG	1 cut(s)	2784
SinI	GGWCC	4 cut(s)	693, 1877, 1902, 2478
SmiMI	CAYNNNNRTG	7 cut(s)	104, 110, 1188, 1479, 1986, 2196, 2210
SmlI	CTYRAG	2 cut(s)	338, 1799
SmoI	CTYRAG	2 cut(s)	338, 1799
SspI	AATATT	1 cut(s)	2386
SspMI	CTAG	4 cut(s)	273, 785, 1737, 2612
StyD4I	CCNGG	5 cut(s)	147, 467, 911, 1051, 2069
StyI	CCWWGG	4 cut(s)	22, 2157, 2191, 2474
TaiI	ACGT	1 cut(s)	16
TaqI	TCGA	4 cut(s)	1027, 1036, 2311, 2733
TatI	WGTACW	4 cut(s)	297, 853, 1319, 1413
TauI	GCSGC	2 cut(s)	2696, 2786
TscAI	CASTG	8 cut(s)	95, 106, 581, 786, 812, 828, 992, 1192
TseFI	GTSAC	5 cut(s)	32, 282, 438, 638, 1622
TseI	GCWGC	6 cut(s)	164, 945, 996, 1421, 1838, 2603
Tsp45I	GTSAC	5 cut(s)	32, 282, 438, 638, 1622
TspGWI	ACGGA	1 cut(s)	2297
TspRI	CASTG	8 cut(s)	95, 106, 581, 786, 812, 828, 992, 1192
Van91I	CCANNNNNTGG	1 cut(s)	2744
VneI	GTGCAC	2 cut(s)	97, 1979
VpaK11BI	GGWCC	4 cut(s)	693, 1877, 1902, 2478
XagI	CCTNNNNNAGG	1 cut(s)	2597
XapI	RAATTY	9 cut(s)	187, 960, 1150, 1246, 1655, 1775, 2360, 2459, 2797
XceI	RCATGY	3 cut(s)	708, 1957, 1989
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1545
XmiI	GTMKAC	3 cut(s)	1921, 1968, 2311
XmnI	GAANNNNTTC	1 cut(s)	684
XspI	CTAG	4 cut(s)	273, 785, 1737, 2612
Zsp2I	ATGCAT	1 cut(s)	1959

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.