Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0011695

Physical Location & Seq

Genomic Coordinates Forward (+)

30028827 .. 30031929

Gene Structure 3103 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1223800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1443 bp

ATGGCGAGTATGACAATATGTGCTCGATTCCGGCCTTTGAGCTCCAAAGAGAGAAGAGATCACGGCGATGCAGTCTGCATTCAACACGTAGACTCCGAAACTTTCAGTTTCAAGGATGAGAAGGATGAAGAATTCACATTTAGCTTTGATAAGGTGTTCTACGAGAAATCTGAGCAGGCACAAGTGTATGAATTTCTAGCTTCGCCTATTGTGCGAGATGCTGTTGATGCAATCAATGGGACGATCGTTACTTATGGACAGACTGGAGCGGGGAAGACATATAGCATGGAGGGGCCTAGCATACTGGTACGTGACGAGCAGAAGAGAGGATTACTTCCAAGAGTTGTTGAGGGTCTGTTTGATTGCATTAAATCTTCTGGAAGAACAATGAAGCACTCAATCAAATTGTCAATGGTAGAGATCTACATGGAAAGAGTAAGGGACCTTTTTGATTTAGCAAAAGACAACATACAGATTAAGGAGAGTAAATCGCAGGGGATATTGTTAAATGGAGTAACAGAGATCTCTGTGTCGAGTCCTGCAGAAGCATTGCAAAGCCTATCTAATGGCATAGCTAACAGAGCTGTGGGAGAGACCCAAATGAATGTGTCCAGCAGCAGAAGCCACTGCATATACATATTCACAGTTCAGCAAGAAGTAAAGAAAGATGAGAGGTCGAAAGCTGGAAAGTTAATTCTTGTAGACTTGGCTGGATCTGAGAAAGTAGAGAAAACTGGAGCTGAGGGAAAAGTTCTTGAAGAAGCTAAGACCATCAACAAGTCTCTCTCAGCCCTTGGAAATGTGATAAATGCTCTGACATGCGGGTCATCGGGCAAAGCAAACCACATTCCCTTCCGCGATTCCAAGCTCACTCGGATTTTACAAGATTCACTGGGAGGTAACTCGCGTACTGCATTGTTGTGTTGTTGCTCTCCAAGTCCTTCTAATGCATTGGAGAGTCTGTCCACTCTTCGCTTTGGGATGAGGGCAAAGCATATAAAGATGTCACCGCGTCTCAAGGGTAACGAAGATAAATCTGCTAAAAGGAACGGACCTCCCTCCCCAACTAAAGACGAGTCATGCGAGAGAATTCTTGGGATGAGATTTGATGCCGAAGATGTGAAGTTACTTGAGGAGTTGTTCACATTGAGCGGAATTCTCTTTGATCCTTCTTCGCCTGAAGAGGTAGACTCAGCATTGGCGGATGTTACTTCACAGACGATTACCTTACTGCAGCAAGCAGTGGAAGATCTTGCATCCACTGTTGAGGAGCTTAAGAGAGAGAATAAGGCACTGAAGAACGAATTAGCAGCTGCTAAAAGATTGGATGCACAAAGCGAAGCGGCTGGAGATACTTCAATTGTTCTGCATAAACCTTCAGGAATTATCAGTTCCGTTGTGTCACGGGTCAGGTCACTCTCTTCTATCAAGTTGTTGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0003006 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005871 GO:0005875 GO:0006810 GO:0006928 GO:0006996 GO:0007017 GO:0007018 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007275 GO:0008104 GO:0008150 GO:0008574 GO:0009553 GO:0009555 GO:0009561 GO:0009791 GO:0009987 GO:0010154 GO:0015630 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022402 GO:0022414 GO:0030705 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0042623 GO:0042802 GO:0042803 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045143 GO:0046907 GO:0046983 GO:0048229 GO:0048285 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051179 GO:0051234 GO:0051276 GO:0051321 GO:0051641 GO:0051649 GO:0061458 GO:0061982 GO:0070192 GO:0071840 GO:0098813 GO:0140013 GO:1903046 GO:1990939

KEGG Pathways

Metabolic & Signaling

ko04144 ko04728 map04144 map04728

Pfam Domains

Protein Families

Kinesin

Protein Analysis

481

Amino Acids

52.48

Weight (kDa)

6.7

Isoelectric Point (pI)

49.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	8 - 149	9.3e-20	Microtubule binding
Kinesin	PF00225	9 - 333	2.2e-106	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011695)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G63480 AT3G63480
fragaria_vesca	FvH4_6g05340 FvH4_6g05340
malus_domestica	MD12G1223800.v1.1
prunus_persica	Prupe.6G330200_v2.0.a1 Prupe.6G330200_v2.0.a1
pyrus_communis	pycom12g20920
rosa_chinensis	RchiOBHm_Chr3g0454101
rosa_laevigata	RLG00000025483
rosa_multiflora	Rmu_sc0020696.1_g000002
rosa_roxburghii	Rroxscaffold_6G00430890
rosa_rugosa	Rorug02G0653000 Rorug02G0653100
rosa_samantha	Rh3AG055900 Rh3BG057300 Rh3CG056600 Rh3DG057900
rosa_wichuraiana	Rw3G004290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	823
AccBSI	CCGCTC	2 cut(s)	269, 1152
AccI	GTMKAC	3 cut(s)	90, 702, 1188
AccII	CGCG	3 cut(s)	858, 907, 1012
AciI	CCGC	7 cut(s)	269, 822, 856, 1010, 1152, 1202, 1343
AclWI	GGATC	2 cut(s)	721, 1160
AcsI	RAATTY	4 cut(s)	131, 191, 1089, 1155
AcuI	CTGAAG	3 cut(s)	1200, 1316, 1362
AfaI	GTAC	2 cut(s)	309, 910
AflII	CTTAAG	1 cut(s)	1274
AflIII	ACRYGT	1 cut(s)	85
AgsI	TTSAA	5 cut(s)	83, 112, 758, 1359, 1438
Alw21I	GWGCWC	2 cut(s)	25, 44
Alw26I	GTCTC	3 cut(s)	587, 786, 1019
AlwI	GGATC	2 cut(s)	721, 1160
AoxI	GGCC	2 cut(s)	32, 293
ApeKI	GCWGC	4 cut(s)	615, 1234, 1310, 1313
ApoI	RAATTY	4 cut(s)	131, 191, 1089, 1155
AspS9I	GGNCC	3 cut(s)	293, 442, 1052
AsuHPI	GGTGA	1 cut(s)	999
AvaII	GGWCC	2 cut(s)	442, 1052
BanII	GRGCYC	1 cut(s)	44
BbsI	GAAGAC	1 cut(s)	281
Bbv12I	GWGCWC	2 cut(s)	25, 44
BbvCI	CCTCAGC	1 cut(s)	741
BbvI	GCAGC	4 cut(s)	627, 1246, 1300, 1322
BccI	CCATC	1 cut(s)	779
BceAI	ACGGC	1 cut(s)	79
BcoDI	GTCTC	3 cut(s)	587, 786, 1019
BfaI	CTAG	2 cut(s)	197, 297
BfmI	CTRYAG	2 cut(s)	540, 1232
BfrI	CTTAAG	1 cut(s)	1274
BglII	AGATCT	3 cut(s)	420, 522, 1249
BisI	GCNGC	5 cut(s)	616, 1235, 1311, 1314, 1344
BlsI	GCNGC	5 cut(s)	617, 1236, 1312, 1315, 1345
Bme18I	GGWCC	2 cut(s)	442, 1052
BmgT120I	GGNCC	3 cut(s)	293, 442, 1052
BmiI	GGNNCC	2 cut(s)	294, 443
BmrI	ACTGGG	1 cut(s)	902
BmsI	GCATC	6 cut(s)	58, 208, 217, 1099, 1265, 1318
BmuI	ACTGGG	1 cut(s)	902
BpiI	GAAGAC	1 cut(s)	281
BplI	GAGNNNNNCTC	2 cut(s)	1175, 1207
BpmI	CTGGAG	3 cut(s)	285, 756, 1368
Bpu10I	CCTNAGC	1 cut(s)	741
BpuEI	CTTGAG	2 cut(s)	1001, 1151
BsaAI	YACGTR	2 cut(s)	88, 311
BsaI	GGTCTC	1 cut(s)	587
BsaJI	CCNNGG	1 cut(s)	793
BsaXI	ACNNNNNCTCC	4 cut(s)	77, 107, 947, 977
Bse1I	ACTGG	4 cut(s)	268, 309, 739, 897
Bse3DI	GCAATG	1 cut(s)	548
BseDI	CCNNGG	1 cut(s)	793
BseGI	GGATG	7 cut(s)	121, 130, 987, 1104, 1210, 1256, 1333
BseMI	GCAATG	1 cut(s)	548
BseMII	CTCAG	5 cut(s)	162, 708, 732, 801, 1206
BseNI	ACTGG	4 cut(s)	268, 309, 739, 897
BseRI	GAGGAG	2 cut(s)	1148, 1283
BseXI	GCAGC	4 cut(s)	627, 1246, 1300, 1322
Bsh1236I	CGCG	3 cut(s)	858, 907, 1012
Bsh1285I	CGRYCG	1 cut(s)	246
BshFI	GGCC	2 cut(s)	34, 295
BsiEI	CGRYCG	1 cut(s)	246
BsiHKAI	GWGCWC	2 cut(s)	25, 44
BsiSI	CCGG	1 cut(s)	31
BslFI	GGGAC	2 cut(s)	253, 455
BsmAI	GTCTC	3 cut(s)	587, 786, 1019
BsmBI	CGTCTC	1 cut(s)	1019
BsmFI	GGGAC	2 cut(s)	253, 455
BsmI	GAATGC	1 cut(s)	78
BsnI	GGCC	2 cut(s)	34, 295
Bso31I	GGTCTC	1 cut(s)	587
Bsp1286I	GDGCHC	2 cut(s)	25, 44
Bsp143I	GATC	7 cut(s)	58, 243, 420, 522, 713, 1165, 1249
BspACI	CCGC	7 cut(s)	269, 822, 856, 1010, 1152, 1202, 1343
BspANI	GGCC	2 cut(s)	34, 295
BspCNI	CTCAG	5 cut(s)	163, 709, 733, 800, 1205
BspFNI	CGCG	3 cut(s)	858, 907, 1012
BspLI	GGNNCC	2 cut(s)	294, 443
BspMAI	CTGCAG	2 cut(s)	544, 1236
BspPI	GGATC	2 cut(s)	721, 1160
BspTI	CTTAAG	1 cut(s)	1274
BspTNI	GGTCTC	1 cut(s)	587
BsrBI	CCGCTC	2 cut(s)	269, 1152
BsrDI	GCAATG	1 cut(s)	548
BsrI	ACTGG	4 cut(s)	268, 309, 739, 897
BssECI	CCNNGG	1 cut(s)	793
BssMI	GATC	7 cut(s)	58, 243, 420, 522, 713, 1165, 1249
BssT1I	CCWWGG	1 cut(s)	793
Bst4CI	ACNGT	2 cut(s)	646, 1264
Bst6I	CTCTTC	5 cut(s)	49, 317, 975, 1176, 1426
BstAFI	CTTAAG	1 cut(s)	1274
BstBAI	YACGTR	2 cut(s)	88, 311
BstC8I	GCNNGC	2 cut(s)	177, 1239
BstDEI	CTNAG	6 cut(s)	171, 717, 741, 765, 787, 1192
BstF5I	GGATG	7 cut(s)	121, 130, 987, 1104, 1210, 1256, 1333
BstFNI	CGCG	3 cut(s)	858, 907, 1012
BstKTI	GATC	7 cut(s)	61, 246, 423, 525, 716, 1168, 1252
BstMAI	GTCTC	3 cut(s)	587, 786, 1019
BstMBI	GATC	7 cut(s)	58, 243, 420, 522, 713, 1165, 1249
BstMCI	CGRYCG	1 cut(s)	246
BstMWI	GCNNNNNNNGC	4 cut(s)	211, 227, 581, 621
BstNSI	RCATGY	1 cut(s)	822
BstSFI	CTRYAG	2 cut(s)	540, 1232
BstUI	CGCG	3 cut(s)	858, 907, 1012
BstV1I	GCAGC	4 cut(s)	627, 1246, 1300, 1322
BstV2I	GAAGAC	1 cut(s)	281
BstX2I	RGATCY	4 cut(s)	420, 522, 713, 1249
BstYI	RGATCY	4 cut(s)	420, 522, 713, 1249
BsuRI	GGCC	2 cut(s)	34, 295
BtgZI	GCGATG	1 cut(s)	81
BtsCI	GGATG	7 cut(s)	121, 130, 987, 1104, 1210, 1256, 1333
BtsI	GCAGTG	2 cut(s)	625, 1248
BtsIMutI	CAGTG	5 cut(s)	625, 890, 1248, 1260, 1292
Cac8I	GCNNGC	2 cut(s)	177, 1239
Cfr13I	GGNCC	3 cut(s)	293, 442, 1052
CseI	GACGC	1 cut(s)	1001
Csp6I	GTAC	2 cut(s)	308, 909
CviAII	CATG	4 cut(s)	286, 427, 819, 1080
CviQI	GTAC	2 cut(s)	308, 909
DdeI	CTNAG	6 cut(s)	171, 717, 741, 765, 787, 1192
DpnI	GATC	7 cut(s)	60, 245, 422, 524, 715, 1167, 1251
DpnII	GATC	7 cut(s)	58, 243, 420, 522, 713, 1165, 1249
DrdI	GACNNNNNNGTC	1 cut(s)	823
DseDI	GACNNNNNNGTC	1 cut(s)	823
Eam1104I	CTCTTC	5 cut(s)	49, 317, 975, 1176, 1426
EarI	CTCTTC	5 cut(s)	49, 317, 975, 1176, 1426
EciI	GGCGGA	1 cut(s)	1217
Ecl136II	GAGCTC	1 cut(s)	42
Eco130I	CCWWGG	1 cut(s)	793
Eco24I	GRGCYC	1 cut(s)	44
Eco31I	GGTCTC	1 cut(s)	587
Eco47I	GGWCC	2 cut(s)	442, 1052
Eco53kI	GAGCTC	1 cut(s)	42
Eco57I	CTGAAG	3 cut(s)	1200, 1316, 1362
EcoICRI	GAGCTC	1 cut(s)	42
EcoO109I	RGGNCCY	2 cut(s)	293, 442
EcoRI	GAATTC	3 cut(s)	131, 1089, 1155
EcoT14I	CCWWGG	1 cut(s)	793
EcoT22I	ATGCAT	1 cut(s)	952
EcoT38I	GRGCYC	1 cut(s)	44
ErhI	CCWWGG	1 cut(s)	793
Esp3I	CGTCTC	1 cut(s)	1019
FaeI	CATG	4 cut(s)	289, 430, 822, 1083
FaqI	GGGAC	2 cut(s)	253, 455
FatI	CATG	4 cut(s)	285, 426, 818, 1079
FauI	CCCGC	2 cut(s)	262, 815
FblI	GTMKAC	3 cut(s)	90, 702, 1188
Fnu4HI	GCNGC	5 cut(s)	616, 1235, 1311, 1314, 1344
FokI	GGATG	7 cut(s)	128, 137, 994, 1111, 1217, 1243, 1340
FriOI	GRGCYC	1 cut(s)	44
Fsp4HI	GCNGC	5 cut(s)	616, 1235, 1311, 1314, 1344
FspBI	CTAG	2 cut(s)	197, 297
GluI	GCNGC	5 cut(s)	616, 1235, 1311, 1314, 1344
GsuI	CTGGAG	3 cut(s)	285, 756, 1368
HaeIII	GGCC	2 cut(s)	34, 295
HapII	CCGG	1 cut(s)	31
HgaI	GACGC	1 cut(s)	1001
Hin1II	CATG	4 cut(s)	289, 430, 822, 1083
HinfI	GANTC	8 cut(s)	27, 92, 535, 860, 887, 958, 1076, 1190
HpaII	CCGG	1 cut(s)	31
HphI	GGTGA	1 cut(s)	999
Hpy166II	GTNNAC	5 cut(s)	91, 703, 966, 1143, 1189
Hpy188I	TCNGA	5 cut(s)	97, 172, 718, 816, 876
Hpy188III	TCNNGA	3 cut(s)	378, 755, 1380
Hpy8I	GTNNAC	5 cut(s)	91, 703, 966, 1143, 1189
HpyAV	CCTTC	5 cut(s)	115, 862, 951, 1179, 1386
HpyCH4III	ACNGT	2 cut(s)	646, 1264
HpyCH4IV	ACGT	2 cut(s)	87, 310
HpyF10VI	GCNNNNNNNGC	4 cut(s)	211, 227, 581, 621
HpyF3I	CTNAG	6 cut(s)	171, 717, 741, 765, 787, 1192
HpySE526I	ACGT	2 cut(s)	87, 310
Hsp92II	CATG	4 cut(s)	289, 430, 822, 1083
Kzo9I	GATC	7 cut(s)	58, 243, 420, 522, 713, 1165, 1249
LmnI	GCTCC	4 cut(s)	47, 266, 737, 1270
Lsp1109I	GCAGC	4 cut(s)	627, 1246, 1300, 1322
LweI	GCATC	6 cut(s)	58, 208, 217, 1099, 1265, 1318
MaeI	CTAG	2 cut(s)	197, 297
MaeII	ACGT	2 cut(s)	87, 310
MalI	GATC	7 cut(s)	60, 245, 422, 524, 715, 1167, 1251
MbiI	CCGCTC	2 cut(s)	269, 1152
MboI	GATC	7 cut(s)	58, 243, 420, 522, 713, 1165, 1249
MfeI	CAATTG	1 cut(s)	1359
MflI	RGATCY	4 cut(s)	420, 522, 713, 1249
MhlI	GDGCHC	2 cut(s)	25, 44
MluCI	AATT	9 cut(s)	131, 191, 404, 693, 1089, 1155, 1304, 1359, 1383
MlyI	GAGTC	5 cut(s)	86, 544, 967, 1085, 1184
Mph1103I	ATGCAT	1 cut(s)	952
MseI	TTAA	5 cut(s)	369, 477, 506, 692, 1275
MslI	CAYNNNNRTG	2 cut(s)	66, 919
MspA1I	CMGCKG	1 cut(s)	1313
MspCI	CTTAAG	1 cut(s)	1274
MspI	CCGG	1 cut(s)	31
MunI	CAATTG	1 cut(s)	1359
Mva1269I	GAATGC	1 cut(s)	78
MvnI	CGCG	3 cut(s)	858, 907, 1012
MwoI	GCNNNNNNNGC	4 cut(s)	211, 227, 581, 621
NdeII	GATC	7 cut(s)	58, 243, 420, 522, 713, 1165, 1249
NlaIII	CATG	4 cut(s)	289, 430, 822, 1083
NlaIV	GGNNCC	2 cut(s)	294, 443
NmuCI	GTSAC	4 cut(s)	311, 1005, 1401, 1413
NsiI	ATGCAT	1 cut(s)	952
NspI	RCATGY	1 cut(s)	822
PcsI	WCGNNNNNNNCGW	2 cut(s)	93, 1080
PctI	GAATGC	1 cut(s)	78
PfeI	GAWTC	3 cut(s)	27, 860, 887
PkrI	GCNGC	5 cut(s)	617, 1236, 1312, 1315, 1345
Ple19I	CGATCG	1 cut(s)	246
PleI	GAGTC	5 cut(s)	86, 543, 966, 1084, 1184
PpsI	GAGTC	5 cut(s)	86, 543, 966, 1084, 1184
Ppu21I	YACGTR	2 cut(s)	88, 311
PpuMI	RGGWCCY	1 cut(s)	442
Psp124BI	GAGCTC	1 cut(s)	44
Psp5II	RGGWCCY	1 cut(s)	442
PspN4I	GGNNCC	2 cut(s)	294, 443
PspPI	GGNCC	3 cut(s)	293, 442, 1052
PspPPI	RGGWCCY	1 cut(s)	442
PstI	CTGCAG	2 cut(s)	544, 1236
PsuI	RGATCY	4 cut(s)	420, 522, 713, 1249
PvuI	CGATCG	1 cut(s)	246
PvuII	CAGCTG	1 cut(s)	1313
RsaI	GTAC	2 cut(s)	309, 910
RsaNI	GTAC	2 cut(s)	308, 909
RseI	CAYNNNNRTG	2 cut(s)	66, 919
SacI	GAGCTC	1 cut(s)	44
SaqAI	TTAA	5 cut(s)	369, 477, 506, 692, 1275
SatI	GCNGC	5 cut(s)	616, 1235, 1311, 1314, 1344
Sau3AI	GATC	7 cut(s)	58, 243, 420, 522, 713, 1165, 1249
Sau96I	GGNCC	3 cut(s)	293, 442, 1052
SchI	GAGTC	5 cut(s)	86, 544, 967, 1085, 1184
SduI	GDGCHC	2 cut(s)	25, 44
SfaNI	GCATC	6 cut(s)	58, 208, 217, 1099, 1265, 1318
SfcI	CTRYAG	2 cut(s)	540, 1232
SinI	GGWCC	2 cut(s)	442, 1052
SmiMI	CAYNNNNRTG	2 cut(s)	66, 919
SmlI	CTYRAG	3 cut(s)	1016, 1130, 1274
SmoI	CTYRAG	3 cut(s)	1016, 1130, 1274
Sse9I	AATT	9 cut(s)	131, 191, 404, 693, 1089, 1155, 1304, 1359, 1383
SsiI	CCGC	7 cut(s)	269, 822, 856, 1010, 1152, 1202, 1343
SspMI	CTAG	2 cut(s)	197, 297
SstI	GAGCTC	1 cut(s)	44
StyI	CCWWGG	1 cut(s)	793
TaaI	ACNGT	2 cut(s)	646, 1264
TaiI	ACGT	2 cut(s)	90, 313
TaqI	TCGA	3 cut(s)	25, 533, 677
TasI	AATT	9 cut(s)	131, 191, 404, 693, 1089, 1155, 1304, 1359, 1383
TauI	GCSGC	1 cut(s)	1346
TfiI	GAWTC	3 cut(s)	27, 860, 887
Tru1I	TTAA	5 cut(s)	369, 477, 506, 692, 1275
Tru9I	TTAA	5 cut(s)	369, 477, 506, 692, 1275
TscAI	CASTG	5 cut(s)	632, 897, 1248, 1267, 1299
TseFI	GTSAC	4 cut(s)	311, 1005, 1401, 1413
TseI	GCWGC	4 cut(s)	615, 1234, 1310, 1313
Tsp45I	GTSAC	4 cut(s)	311, 1005, 1401, 1413
TspDTI	ATGAA	4 cut(s)	141, 204, 404, 617
TspGWI	ACGGA	2 cut(s)	1065, 1384
TspRI	CASTG	5 cut(s)	632, 897, 1248, 1267, 1299
Vha464I	CTTAAG	1 cut(s)	1274
VpaK11BI	GGWCC	2 cut(s)	442, 1052
XapI	RAATTY	4 cut(s)	131, 191, 1089, 1155
XceI	RCATGY	1 cut(s)	822
XmiI	GTMKAC	3 cut(s)	90, 702, 1188
XspI	CTAG	2 cut(s)	197, 297
Zsp2I	ATGCAT	1 cut(s)	952

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.