RLG00000025483

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0011695
Physical Location & Seq
Forward (+)
46017096 .. 46023565
6470 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025483

Sequence Viewer

Length: 2424 bp
ATGTCGAGCATAACAGCTTGCGTTCGGTTCAGGCCTTTGAGCTCCAGAGAGAGAAGAGATCACGGCGATTCGGTCTGCATACACTGCGTAGATTCGCAGACTTTCGTCTTCAAGGATGAGAAGGAAGAGGAATTTACGTTTAGCTTTGATAAGGTTTTTTACGAAAACTCGGAGCAAGCTCACGTCTATGAATATCTAGCTCTGCCTATTGTGAAAGATGCTGTTGATGCTATCAACGGCACGATCATCACTTATGGACAGACTGGAGCTGGGAAGACGTATAGTATGGAGGGGCCTAGCATAATGGTATGTGACGAGCAGAAGAAAGGATTAGTTCCCAGAGTAGTTGATGGACTGTTTGATTGCATTAAGTTTTCTGAAGGAACAGTGAAGTACTCGATCAAATTGTCAATGGTGGAGATCTACATGGAAAAAGTTAGGGACCTCTTTGATTTGTCGAAAGACAACATACAGATCAAGGAGAGTAAAGCTCAGGGCATAGTGTTAAATGGAGTGACAGAGATCTCTGTATCGGATCCTGCAGAAGCATTACAGATCCTATCTAGTGGAATAGCTAACAGAACTTTTGGAGAGACCCAGATGAACATGTCCAGCAGCAGAAGTCATTGTATTTACCTATTCACAGTTCAGCAAGAATTAAAGAAAGATAACAGGTTGAAAACTGGCAAATTGATTCTTGTTGACTTGGCTGGTTCTGAGAAAGCAGAGAAAACTGGAGCAGAGGGAAAAGTTCTTGAAGAAGCCAAGACCATCAACAAATCCCTCTCAGCCCTTGGAAATGTGATAAATGCTCTCACATGTGGTTCACCAGGCAAAGCATACCATATCCCATATCGTGATTCCAAACTCACTCGGCTTTTACAAGATGCACTGGGAGGAAACTCCCGTACTGCATTGTTGTGCTGTTGCTCACCAAGCCCTTCAAATGCATCAGAGAGTCTGTCCACTCTTCGCTTTGGAATGAGGGCAAAACATATAAAGATGTCACTGCATGCCAAGTCCAGTGAAGATAAGTATGCTAAAAAGGATGGATCTCCCTCTTCAAGTAAAAACCAGGCCTGTGAGAGAATTTTGGACCAGTTGAGGGACAGATTTGATGTTGAAGATGTGGTGTTACTTGAGGAGTTGTTCAGATTGAGTGGAATTCTCTATGATCCTGGTTCACCTGAAGATTTGGATTCGGCATTTGAGGATGTCACTTCACAGACAATTATCCAATTGCAGCACATGGTGGAAGAGCTTGTATCAACTGTAGAGGAGCTCAAGAACGAGAATATGACTCTGAAGGACAGACTGGCAGCTGGTGAAAGATCCGATACACAACGCAAAGAAGCTGGAGGTCATATTTCAAGCCTTCTGCATAGAGTTTCAGGCACTCTCGGTGAACTTGCTGCTGGCCTTGGTTTCGAGCTCCCACCTCCATATGGACAGACTTACCCCAAACTTTCACCAATCGGAAGGGCTTGTGATGGCCGTCTTATTGTAGACTTTCTAATGGATGCAATGAACCTGCCATTTCTAAATGCCTACTTAGATTCAGTTGGAATGCCGAGTTTCCGAAGAGGGTGTAACTTTGCTGCTGCAGGGTCTACTATACTTCCAGCTTCTCCAACATCTGTGAGCCCATTTTCATTTGCTGTCCAAGTCTCTCAGTTTCTTAGATTCAAAGCCAGGGTTCTTGAAATTCTAGCTGAAGGTAAGAAATTTGAAAAGTATCTGCCCACAGAAGATTTTTTTGGGAAGGGGCTTTACATGTTTGACATTGGCCAAAATGACATTGCTGGTGGATTTTACTCTAAGACATTAGATCAGATTCTCGCTTCAATCCCAACAATTCTGGCAGAATTCGAAACAGGAATAAAGAGACTATACGATCAAGGGGCTAGGAACTTTTGGATACACAATACAGGTCCTGTGGGATGCTTGACTCAGAATGTTGCCAAATTCGGAACAGACCCCTCAAAGCTTGATGATCAAGGATGTGTTAGCGCACATAACCAAGCTGCCAAAACTTTTAACCTGCAGCTTCATGCTCTCACCAAAAAGTTGCAGGGCCAATATACTGATGCAAATGTCACATATGTTGATATCTTCTCTATAAAATCTAACCTCATTGCAAACTATTCAAAAAATGGATTCGAACAACCTATTATGGCTTGCTGTGGTTATGGAGGTCCACCTTTGAACTATGACAGTCAGATAAGCTGTGGGCAAACGAAGGTCTTGAATGGGAGCTCTGTCACAGCCAAAGGGTGCAGTGACAGTAGTGCCTATGTTAGTTGGGATGGGATTCATTACACTGAGGCTGCAAATCAGTTTGTCTCATCAAAGATACTCACCGGAATATATTCTGATCCACCCTTTTCAGACCAAATGCCTTTCCTTCTGGACCTCAAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 ko04728 map04144 map04728
Pfam Domains
Protein Families
Kinesin Lipase_GDSL

Protein Analysis

808

Amino Acids

88.69

Weight (kDa)

5.69

Isoelectric Point (pI)

45.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kinesin PF00225 9 - 333 9.4e-105 Kinesin motor domain
Microtub_bd PF16796 24 - 149 8.3e-20 Microtubule binding
Lipase_GDSL PF00657 490 - 784 3.2e-39 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011695)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63480 AT3G63480
fragaria_vesca FvH4_6g05340 FvH4_6g05340
malus_domestica MD12G1223800.v1.1
prunus_persica Prupe.6G330200_v2.0.a1 Prupe.6G330200_v2.0.a1
pyrus_communis pycom12g20920
rosa_chinensis RchiOBHm_Chr3g0454101
rosa_laevigata RLG00000025483
rosa_multiflora Rmu_sc0020696.1_g000002
rosa_roxburghii Rroxscaffold_6G00430890
rosa_rugosa Rorug02G0653000 Rorug02G0653100
rosa_samantha Rh3AG055900 Rh3BG057300 Rh3CG056600 Rh3DG057900
rosa_wichuraiana Rw3G004290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1539, 2049
AccI GTMKAC 2 cut(s) 1506, 1610
AclWI GGATC 7 cut(s) 530, 543, 550, 1060, 1169, 1326, 2369
AcoI YGGCCR 2 cut(s) 1492, 1786
AcsI RAATTY 7 cut(s) 131, 1089, 1164, 1704, 1724, 1865, 1964
AcuI CTGAAG 4 cut(s) 399, 1209, 1325, 1734
AdeI CACNNNGTG 1 cut(s) 1252
AfaI GTAC 2 cut(s) 395, 910
AfiI CCNNNNNNNGG 2 cut(s) 1105, 1445
AflIII ACRYGT 3 cut(s) 606, 818, 1773
AjiI CACGTC 1 cut(s) 184
AjnI CCWGG 4 cut(s) 829, 1074, 1177, 1691
AjuI GAANNNNNNNTTGG 2 cut(s) 1740, 1772
AloI GAACNNNNNNTCC 1 cut(s) 2402
Alw21I GWGCWC 4 cut(s) 44, 1284, 1434, 2258
Alw26I GTCTC 4 cut(s) 587, 1672, 1879, 2347
AlwI GGATC 7 cut(s) 530, 543, 550, 1060, 1169, 1326, 2369
AlwNI CAGNNNCTG 1 cut(s) 1934
AoxI GGCC 7 cut(s) 32, 293, 1077, 1417, 1492, 1786, 2074
ApeKI GCWGC 9 cut(s) 615, 1243, 1319, 1412, 1598, 1601, 2024, 2044, 2327
ApoI RAATTY 7 cut(s) 131, 1089, 1164, 1704, 1724, 1865, 1964
Asp700I GAANNNNTTC 1 cut(s) 2368
AspLEI GCGC 1 cut(s) 2012
AspS9I GGNCC 7 cut(s) 293, 442, 1096, 1931, 2074, 2195, 2410
AsuHPI GGTGA 8 cut(s) 819, 924, 1176, 1337, 1415, 1461, 2050, 2350
AsuII TTCGAA 2 cut(s) 1869, 2160
AvaII GGWCC 5 cut(s) 442, 1096, 1931, 2195, 2410
BalI TGGCCA 1 cut(s) 1788
BamHI GGATCC 1 cut(s) 535
BanII GRGCYC 5 cut(s) 44, 1284, 1434, 1646, 2258
BarI GAAGNNNNNNTAC 2 cut(s) 1754, 1786
BbsI GAAGAC 2 cut(s) 100, 281
Bbv12I GWGCWC 4 cut(s) 44, 1284, 1434, 2258
BbvI GCAGC 9 cut(s) 627, 1255, 1331, 1399, 1585, 1588, 2011, 2056, 2314
BccI CCATC 5 cut(s) 344, 779, 1043, 1484, 2300
BceAI ACGGC 3 cut(s) 79, 253, 1479
BciT130I CCWGG 4 cut(s) 831, 1076, 1179, 1693
BciVI GTATCC 1 cut(s) 1911
BclI TGATCA 1 cut(s) 1993
BcoDI GTCTC 4 cut(s) 587, 1672, 1879, 2347
BfaI CTAG 5 cut(s) 197, 297, 564, 1709, 1905
BfmI CTRYAG 4 cut(s) 540, 1272, 1602, 2042
BfuAI ACCTGC 2 cut(s) 1539, 2049
BfuI GTATCC 1 cut(s) 1911
BglII AGATCT 2 cut(s) 420, 522
BisI GCNGC 9 cut(s) 616, 1244, 1320, 1413, 1599, 1602, 2025, 2045, 2328
BlsI GCNGC 9 cut(s) 617, 1245, 1321, 1414, 1600, 1603, 2026, 2046, 2329
BmcAI AGTACT 1 cut(s) 395
Bme1390I CCNGG 4 cut(s) 831, 1076, 1179, 1693
Bme18I GGWCC 5 cut(s) 442, 1096, 1931, 2195, 2410
BmgBI CACGTC 1 cut(s) 184
BmgT120I GGNCC 7 cut(s) 293, 442, 1096, 1931, 2074, 2195, 2410
BmiI GGNNCC 3 cut(s) 294, 443, 537
BmrFI CCNGG 4 cut(s) 831, 1076, 1179, 1693
BmrI ACTGGG 1 cut(s) 902
BmsI GCATC 7 cut(s) 208, 217, 877, 959, 1510, 1931, 2077
BmuI ACTGGG 1 cut(s) 902
BoxI GACNNNNGTC 1 cut(s) 104
BpiI GAAGAC 2 cut(s) 100, 281
BplI GAGNNNNNCTC 2 cut(s) 475, 507
BpmI CTGGAG 4 cut(s) 28, 285, 756, 1377
Bpu10I CCTNAGC 1 cut(s) 492
Bpu14I TTCGAA 2 cut(s) 1869, 2160
BpuEI CTTGAG 3 cut(s) 1160, 1268, 2399
BsaI GGTCTC 1 cut(s) 587
BsaJI CCNNGG 3 cut(s) 793, 1420, 1692
BsaWI WCCGGW 1 cut(s) 2360
Bsc4I CCNNNNNNNGG 2 cut(s) 1105, 1445
Bse1I ACTGG 7 cut(s) 268, 688, 739, 897, 1023, 1099, 1320
Bse3DI GCAATG 3 cut(s) 1530, 1797, 2133
BseBI CCWGG 4 cut(s) 831, 1076, 1179, 1693
BseDI CCNNGG 3 cut(s) 793, 1420, 1692
BseGI GGATG 7 cut(s) 121, 1054, 1219, 1525, 1946, 2006, 2311
BseLI CCNNNNNNNGG 2 cut(s) 1105, 1445
BseMI GCAATG 3 cut(s) 1530, 1797, 2133
BseMII CTCAG 6 cut(s) 506, 708, 801, 1685, 1964, 2313
BseNI ACTGG 7 cut(s) 268, 688, 739, 897, 1023, 1099, 1320
BseRI GAGGAG 2 cut(s) 1157, 1292
BseXI GCAGC 9 cut(s) 627, 1255, 1331, 1399, 1585, 1588, 2011, 2056, 2314
BseYI CCCAGC 1 cut(s) 269
BsgI GTGCAG 1 cut(s) 2296
BshFI GGCC 7 cut(s) 34, 295, 1079, 1419, 1494, 1788, 2076
BsiHKAI GWGCWC 4 cut(s) 44, 1284, 1434, 2258
BsiSI CCGG 1 cut(s) 2361
BslFI GGGAC 2 cut(s) 455, 1121
BslI CCNNNNNNNGG 2 cut(s) 1105, 1445
BsmAI GTCTC 4 cut(s) 587, 1672, 1879, 2347
BsmFI GGGAC 2 cut(s) 455, 1121
BsmI GAATGC 1 cut(s) 1572
BsnI GGCC 7 cut(s) 34, 295, 1079, 1419, 1494, 1788, 2076
Bso31I GGTCTC 1 cut(s) 587
Bsp119I TTCGAA 2 cut(s) 1869, 2160
Bsp1286I GDGCHC 5 cut(s) 44, 1284, 1434, 1646, 2258
BspANI GGCC 7 cut(s) 34, 295, 1079, 1419, 1494, 1788, 2076
BspCNI CTCAG 6 cut(s) 505, 709, 800, 1684, 1963, 2314
BspLI GGNNCC 3 cut(s) 294, 443, 537
BspMAI CTGCAG 3 cut(s) 544, 1606, 2046
BspMI ACCTGC 2 cut(s) 1539, 2049
BspPI GGATC 7 cut(s) 530, 543, 550, 1060, 1169, 1326, 2369
BspQI GCTCTTC 1 cut(s) 1251
BspT104I TTCGAA 2 cut(s) 1869, 2160
BspTNI GGTCTC 1 cut(s) 587
BsrDI GCAATG 3 cut(s) 1530, 1797, 2133
BsrI ACTGG 7 cut(s) 268, 688, 739, 897, 1023, 1099, 1320
BssECI CCNNGG 3 cut(s) 793, 1420, 1692
BssT1I CCWWGG 2 cut(s) 793, 1420
Bst2UI CCWGG 4 cut(s) 831, 1076, 1179, 1693
Bst4CI ACNGT 6 cut(s) 357, 388, 646, 1273, 2216, 2285
Bst6I CTCTTC 6 cut(s) 49, 120, 975, 1066, 1251, 1576
BstAPI GCANNNNNTGC 1 cut(s) 84
BstBI TTCGAA 2 cut(s) 1869, 2160
BstC8I GCNNGC 5 cut(s) 19, 177, 1014, 1417, 2179
BstDEI CTNAG 9 cut(s) 492, 717, 787, 1552, 1671, 1679, 1818, 1950, 2322
BstF5I GGATG 7 cut(s) 121, 1054, 1219, 1525, 1946, 2006, 2311
BstHHI GCGC 1 cut(s) 2012
BstMAI GTCTC 4 cut(s) 587, 1672, 1879, 2347
BstMWI GCNNNNNNNGC 3 cut(s) 84, 227, 936
BstNI CCWGG 4 cut(s) 831, 1076, 1179, 1693
BstNSI RCATGY 4 cut(s) 610, 822, 1016, 1777
BstPAI GACNNNNGTC 1 cut(s) 104
BstSCI CCNGG 4 cut(s) 829, 1074, 1177, 1691
BstSFI CTRYAG 4 cut(s) 540, 1272, 1602, 2042
BstV1I GCAGC 9 cut(s) 627, 1255, 1331, 1399, 1585, 1588, 2011, 2056, 2314
BstV2I GAAGAC 2 cut(s) 100, 281
BstX2I RGATCY 6 cut(s) 420, 522, 535, 555, 1052, 1331
BstYI RGATCY 6 cut(s) 420, 522, 535, 555, 1052, 1331
BsuI GTATCC 1 cut(s) 1911
BsuRI GGCC 7 cut(s) 34, 295, 1079, 1419, 1494, 1788, 2076
BtrI CACGTC 1 cut(s) 184
BtsCI GGATG 7 cut(s) 121, 1054, 1219, 1525, 1946, 2006, 2311
BtsI GCAGTG 3 cut(s) 82, 1007, 2284
BtsIMutI CAGTG 7 cut(s) 82, 393, 890, 1007, 1030, 2284, 2319
BveI ACCTGC 2 cut(s) 1539, 2049
Cac8I GCNNGC 5 cut(s) 19, 177, 1014, 1417, 2179
CaiI CAGNNNCTG 1 cut(s) 1934
CfoI GCGC 1 cut(s) 2012
Cfr13I GGNCC 7 cut(s) 293, 442, 1096, 1931, 2074, 2195, 2410
Csp6I GTAC 2 cut(s) 394, 909
CviAII CATG 7 cut(s) 427, 607, 819, 1013, 1249, 1774, 2051
CviQI GTAC 2 cut(s) 394, 909
DdeI CTNAG 9 cut(s) 492, 717, 787, 1552, 1671, 1679, 1818, 1950, 2322
DraIII CACNNNGTG 1 cut(s) 1252
EaeI YGGCCR 2 cut(s) 1492, 1786
Eam1104I CTCTTC 6 cut(s) 49, 120, 975, 1066, 1251, 1576
EarI CTCTTC 6 cut(s) 49, 120, 975, 1066, 1251, 1576
Ecl136II GAGCTC 4 cut(s) 42, 1282, 1432, 2256
Eco130I CCWWGG 2 cut(s) 793, 1420
Eco147I AGGCCT 2 cut(s) 34, 1079
Eco24I GRGCYC 5 cut(s) 44, 1284, 1434, 1646, 2258
Eco31I GGTCTC 1 cut(s) 587
Eco32I GATATC 1 cut(s) 2110
Eco47I GGWCC 5 cut(s) 442, 1096, 1931, 2195, 2410
Eco53kI GAGCTC 4 cut(s) 42, 1282, 1432, 2256
Eco57I CTGAAG 4 cut(s) 399, 1209, 1325, 1734
EcoICRI GAGCTC 4 cut(s) 42, 1282, 1432, 2256
EcoO109I RGGNCCY 3 cut(s) 293, 442, 1931
EcoRI GAATTC 2 cut(s) 1164, 1865
EcoRII CCWGG 4 cut(s) 829, 1074, 1177, 1691
EcoRV GATATC 1 cut(s) 2110
EcoT14I CCWWGG 2 cut(s) 793, 1420
EcoT22I ATGCAT 1 cut(s) 952
EcoT38I GRGCYC 5 cut(s) 44, 1284, 1434, 1646, 2258
ErhI CCWWGG 2 cut(s) 793, 1420
FaeI CATG 7 cut(s) 430, 610, 822, 1016, 1252, 1777, 2054
FaqI GGGAC 2 cut(s) 455, 1121
FatI CATG 7 cut(s) 426, 606, 818, 1012, 1248, 1773, 2050
FauNDI CATATG 2 cut(s) 1444, 2101
FbaI TGATCA 1 cut(s) 1993
FblI GTMKAC 2 cut(s) 1506, 1610
Fnu4HI GCNGC 9 cut(s) 616, 1244, 1320, 1413, 1599, 1602, 2025, 2045, 2328
FokI GGATG 7 cut(s) 128, 1061, 1226, 1532, 1953, 2013, 2318
FriOI GRGCYC 5 cut(s) 44, 1284, 1434, 1646, 2258
Fsp4HI GCNGC 9 cut(s) 616, 1244, 1320, 1413, 1599, 1602, 2025, 2045, 2328
FspBI CTAG 5 cut(s) 197, 297, 564, 1709, 1905
GlaI GCGC 1 cut(s) 2011
GluI GCNGC 9 cut(s) 616, 1244, 1320, 1413, 1599, 1602, 2025, 2045, 2328
GsaI CCCAGC 1 cut(s) 273
GsuI CTGGAG 4 cut(s) 28, 285, 756, 1377
HaeIII GGCC 7 cut(s) 34, 295, 1079, 1419, 1494, 1788, 2076
HapII CCGG 1 cut(s) 2361
HhaI GCGC 1 cut(s) 2012
Hin1II CATG 7 cut(s) 430, 610, 822, 1016, 1252, 1777, 2054
Hin6I GCGC 1 cut(s) 2010
HinP1I GCGC 1 cut(s) 2010
HincII GTYRAC 1 cut(s) 703
HindII GTYRAC 1 cut(s) 703
HindIII AAGCTT 1 cut(s) 1985
HpaII CCGG 1 cut(s) 2361
HphI GGTGA 8 cut(s) 819, 924, 1176, 1337, 1415, 1461, 2050, 2350
Hpy166II GTNNAC 8 cut(s) 703, 827, 966, 1184, 1406, 1507, 1611, 2198
Hpy188III TCNNGA 7 cut(s) 45, 755, 857, 1285, 1700, 2245, 2408
Hpy8I GTNNAC 8 cut(s) 703, 827, 966, 1184, 1406, 1507, 1611, 2198
HpyCH4III ACNGT 6 cut(s) 357, 388, 646, 1273, 2216, 2285
HpyCH4IV ACGT 3 cut(s) 137, 183, 278
HpyF10VI GCNNNNNNNGC 3 cut(s) 84, 227, 936
HpyF3I CTNAG 9 cut(s) 492, 717, 787, 1552, 1671, 1679, 1818, 1950, 2322
HpySE526I ACGT 3 cut(s) 137, 183, 278
Hsp92II CATG 7 cut(s) 430, 610, 822, 1016, 1252, 1777, 2054
HspAI GCGC 1 cut(s) 2010
Ksp22I TGATCA 1 cut(s) 1993
LguI GCTCTTC 1 cut(s) 1251
LmnI GCTCC 7 cut(s) 47, 172, 266, 737, 1279, 1437, 2253
Lsp1109I GCAGC 9 cut(s) 627, 1255, 1331, 1399, 1585, 1588, 2011, 2056, 2314
LweI GCATC 7 cut(s) 208, 217, 877, 959, 1510, 1931, 2077
MaeI CTAG 5 cut(s) 197, 297, 564, 1709, 1905
MaeII ACGT 3 cut(s) 137, 183, 278
MaeIII GTNAC 9 cut(s) 311, 514, 1005, 1134, 1216, 1589, 2095, 2260, 2279
MfeI CAATTG 1 cut(s) 1238
MflI RGATCY 6 cut(s) 420, 522, 535, 555, 1052, 1331
MhlI GDGCHC 5 cut(s) 44, 1284, 1434, 1646, 2258
MlsI TGGCCA 1 cut(s) 1788
MluNI TGGCCA 1 cut(s) 1788
MlyI GAGTC 3 cut(s) 967, 1294, 1942
MmeI TCCRAC 2 cut(s) 1543, 1655
Mox20I TGGCCA 1 cut(s) 1788
Mph1103I ATGCAT 1 cut(s) 952
MroXI GAANNNNTTC 1 cut(s) 2368
MscI TGGCCA 1 cut(s) 1788
MseI TTAA 4 cut(s) 369, 506, 659, 2037
MslI CAYNNNNRTG 2 cut(s) 186, 919
Msp20I TGGCCA 1 cut(s) 1788
MspA1I CMGCKG 1 cut(s) 1322
MspI CCGG 1 cut(s) 2361
MspR9I CCNGG 4 cut(s) 831, 1076, 1179, 1693
MunI CAATTG 1 cut(s) 1238
Mva1269I GAATGC 1 cut(s) 1572
MvaI CCWGG 4 cut(s) 831, 1076, 1179, 1693
MwoI GCNNNNNNNGC 3 cut(s) 84, 227, 936
NdeI CATATG 2 cut(s) 1444, 2101
NlaIII CATG 7 cut(s) 430, 610, 822, 1016, 1252, 1777, 2054
NlaIV GGNNCC 3 cut(s) 294, 443, 537
NmeAIII GCCGAG 2 cut(s) 853, 1596
NmuCI GTSAC 7 cut(s) 311, 514, 1005, 1216, 2095, 2260, 2279
NsiI ATGCAT 1 cut(s) 952
NspI RCATGY 4 cut(s) 610, 822, 1016, 1777
NspV TTCGAA 2 cut(s) 1869, 2160
PaeI GCATGC 1 cut(s) 1016
PceI AGGCCT 2 cut(s) 34, 1079
PciI ACATGT 3 cut(s) 606, 818, 1773
PciSI GCTCTTC 1 cut(s) 1251
PctI GAATGC 1 cut(s) 1572
PdmI GAANNNNTTC 1 cut(s) 2368
PkrI GCNGC 9 cut(s) 617, 1245, 1321, 1414, 1600, 1603, 2026, 2046, 2329
PleI GAGTC 3 cut(s) 966, 1294, 1942
PpsI GAGTC 3 cut(s) 966, 1294, 1942
PpuMI RGGWCCY 2 cut(s) 442, 1931
PscI ACATGT 3 cut(s) 606, 818, 1773
PshAI GACNNNNGTC 1 cut(s) 104
Psp124BI GAGCTC 4 cut(s) 44, 1284, 1434, 2258
Psp5II RGGWCCY 2 cut(s) 442, 1931
Psp6I CCWGG 4 cut(s) 829, 1074, 1177, 1691
PspFI CCCAGC 1 cut(s) 269
PspGI CCWGG 4 cut(s) 829, 1074, 1177, 1691
PspN4I GGNNCC 3 cut(s) 294, 443, 537
PspPI GGNCC 7 cut(s) 293, 442, 1096, 1931, 2074, 2195, 2410
PspPPI RGGWCCY 2 cut(s) 442, 1931
PstI CTGCAG 3 cut(s) 544, 1606, 2046
PstNI CAGNNNCTG 1 cut(s) 1934
PsuI RGATCY 6 cut(s) 420, 522, 535, 555, 1052, 1331
PvuII CAGCTG 1 cut(s) 1322
RsaI GTAC 2 cut(s) 395, 910
RsaNI GTAC 2 cut(s) 394, 909
RseI CAYNNNNRTG 2 cut(s) 186, 919
SacI GAGCTC 4 cut(s) 44, 1284, 1434, 2258
SapI GCTCTTC 1 cut(s) 1251
SaqAI TTAA 4 cut(s) 369, 506, 659, 2037
SatI GCNGC 9 cut(s) 616, 1244, 1320, 1413, 1599, 1602, 2025, 2045, 2328
Sau96I GGNCC 7 cut(s) 293, 442, 1096, 1931, 2074, 2195, 2410
ScaI AGTACT 1 cut(s) 395
SchI GAGTC 3 cut(s) 967, 1294, 1942
ScrFI CCNGG 4 cut(s) 831, 1076, 1179, 1693
SduI GDGCHC 5 cut(s) 44, 1284, 1434, 1646, 2258
SfaNI GCATC 7 cut(s) 208, 217, 877, 959, 1510, 1931, 2077
SfcI CTRYAG 4 cut(s) 540, 1272, 1602, 2042
SfuI TTCGAA 2 cut(s) 1869, 2160
SinI GGWCC 5 cut(s) 442, 1096, 1931, 2195, 2410
SmiMI CAYNNNNRTG 2 cut(s) 186, 919
SmlI CTYRAG 3 cut(s) 1139, 1283, 2414
SmoI CTYRAG 3 cut(s) 1139, 1283, 2414
SphI GCATGC 1 cut(s) 1016
SseBI AGGCCT 2 cut(s) 34, 1079
SspMI CTAG 5 cut(s) 197, 297, 564, 1709, 1905
SstI GAGCTC 4 cut(s) 44, 1284, 1434, 2258
StuI AGGCCT 2 cut(s) 34, 1079
StyD4I CCNGG 4 cut(s) 829, 1074, 1177, 1691
StyI CCWWGG 2 cut(s) 793, 1420
TaaI ACNGT 6 cut(s) 357, 388, 646, 1273, 2216, 2285
TaiI ACGT 3 cut(s) 140, 186, 281
TaqI TCGA 6 cut(s) 5, 398, 458, 1428, 1869, 2160
TaqII GACCGA 1 cut(s) 61
TatI WGTACW 1 cut(s) 393
Tru1I TTAA 4 cut(s) 369, 506, 659, 2037
Tru9I TTAA 4 cut(s) 369, 506, 659, 2037
TscAI CASTG 7 cut(s) 89, 393, 897, 1014, 1030, 2284, 2326
TseFI GTSAC 7 cut(s) 311, 514, 1005, 1216, 2095, 2260, 2279
TseI GCWGC 9 cut(s) 615, 1243, 1319, 1412, 1598, 1601, 2024, 2044, 2327
Tsp45I GTSAC 7 cut(s) 311, 514, 1005, 1216, 2095, 2260, 2279
TspDTI ATGAA 6 cut(s) 204, 617, 1541, 1641, 2039, 2303
TspRI CASTG 7 cut(s) 89, 393, 897, 1014, 1030, 2284, 2326
VpaK11BI GGWCC 5 cut(s) 442, 1096, 1931, 2195, 2410
XapI RAATTY 7 cut(s) 131, 1089, 1164, 1704, 1724, 1865, 1964
XceI RCATGY 4 cut(s) 610, 822, 1016, 1777
XmiI GTMKAC 2 cut(s) 1506, 1610
XmnI GAANNNNTTC 1 cut(s) 2368
XspI CTAG 5 cut(s) 197, 297, 564, 1709, 1905
ZrmI AGTACT 1 cut(s) 395
Zsp2I ATGCAT 1 cut(s) 952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.