Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0005220

Physical Location & Seq

Genomic Coordinates Reverse (-)

30087141 .. 30091052

Gene Structure 3912 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1225300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1488 bp

ATGAGCAGAGAAAACATAGAAGAAGGAGAACTCTCAAATCCTTTGCTCGTCAAAAATACTACATCTAAGCTCCATAGTGGCGGCGGCGGCAGCGGTGGCTGCAGTGGCAACGGTGGTGCTAGTTATGAAGAAGGAGGTGAAAGTAGCTCATCAGCCACGATTATGGTTGTGGTCAGCACTCTGGTTGCTGTCTCTGGCTCCTATGTTTTTGGGTCTGCGGTGGGATATTCCTCACCGGCTCGGTCTGGGGTCATGAATGATCTGGGCCTCACTGTAGCTCAGTACTCGGTTTTTGGTTCAATATTGACAATCGGAGCAATGATAGGAGCAGTAGTGAGTGGAAGAATAGCAGATTATATAGGCCGAAGAGGTGCAATGGCATTTTCCGAGATATTCTGCATTTTGGGATGGCTAGCAATAGCTTTCTCTAAGGTTGCTTGGTGGGCGGAACTTGGAAGACTGTTGGTTGGATGTGGAATGGGGCTTCTTTCTTATGTGGTGCCTATATACATAGCAGAAATAACGCCGAAGGATCTGCGGGGAGGATTTACAACTGTTCATCAGTTGATGATATGCTGTGGTGTGTCGCTGACATATCTTATCGGGGCTTTTTTGAACTGGCGAGCTTTGGCTCTCCTCGGGACTATTCCGTGTGTTATACAGATTTTAGGTCTCTTCTTCATTCCAGAGTCTCCTAGATGGCTGGCAAAGATCGGTAAATGGAAAGAGTGTGAAGCCGCTCTTCAGCGCCTTAGGGGGGAGAATTCCAATACTTCTCAAGAAGTAGCTGAGATTAGAGATTATACAGAAACTCTGAAACGGCTTTCAGAAGCGAGAATTCTTGACTTATTTCAAAGGAGATACGCTTATTCCCTCATTGTAGGAGTTGGGCTTATGGTACTACAACAATTCGGAGGGGTCAATGGCATTGCCTTCTATGCAAGTACTATATTCATTTCAGCTGGATTTTCCGATAGTGTTGGGACTATAGCTATGGTTGCAGTTCAGGTTCCAATGACAGCATTGGGTGTAATTTTGATGGACAAAGCTGGACGGCGTCCTCTTCTTCTGGTATCTTCAGCAGGAACATGCTTAGGTTGCCTCCTTGTCGGGTTGTCATTCTCGTTGCAGAACCTTCAACAGTGGAAGCAGATTACTCCCATTTTGGCACTTGTTGGCGTTTTGGTATTTACTGGATCGTTCTCGTTGGGCATGGGAGGCATTCCTTGGGTTATAATGTCAGAGATATTTCCCATAAACATGAAGGGGTCAGCCGGTAGCCTAATAACATTGGTTAGCTGGTTAGGTTCTTGGATAGTCTCATACACTTTTAATTTCCTAATGGATTGGAGCTCCGCAGGAACTTTTTTCTTGTTTTCTAGCGTATCTGCTGTAACCGTTCTGTTTGTTGCAAAGCTGGTACCGGAGACAAAGGGGCGAACGCTGGAAGAAATTCAAACATCATTGAACCGATTTTCAGAAAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005351 GO:0005355 GO:0005402 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0008643 GO:0008645 GO:0015075 GO:0015077 GO:0015078 GO:0015144 GO:0015145 GO:0015149 GO:0015291 GO:0015293 GO:0015294 GO:0015295 GO:0015318 GO:0015672 GO:0015749 GO:0022804 GO:0022857 GO:0022890 GO:0034219 GO:0034220 GO:0046323 GO:0051119 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0098655 GO:0098660 GO:0098662 GO:1902600 GO:1904659

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sugar_tr

Protein Analysis

496

Amino Acids

52.85

Weight (kDa)

8.02

Isoelectric Point (pI)

38.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MFS_1	PF07690	72 - 437	4.5e-27	Major Facilitator Superfamily
Sugar_tr	PF00083	81 - 487	1.3e-93	Sugar (and other) transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005220)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54730 AT1G54730 AT1G54730 AT1G54730 AT1G54730 AT1G54730 AT1G54730 AT1G54730 AT1G54730 AT1G54730
fragaria_vesca	FvH4_6g05130 FvH4_6g05130
malus_domestica	MD12G1225300.v1.1
prunus_persica	Prupe.6G331800_v2.0.a1
pyrus_communis	pycom12g21040
rosa_chinensis	RchiOBHm_Chr3g0453671
rosa_laevigata	RLG00000025500
rosa_multiflora	Rmu_sc0003556.1_g000005
rosa_roxburghii	Rroxscaffold_6G00430590
rosa_rugosa	Rorug02G0650600 Rorug02G0650700 Rorug02G0650800.1 Rorug02G0650900 Rorug02G0651000
rosa_samantha	Rh3AG053600 Rh3BG055100 Rh3CG054300 Rh3DG055400
rosa_wichuraiana	Rw3G004110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1235
Acc65I	GGTACC	1 cut(s)	1420
AccB1I	GGYRCC	2 cut(s)	499, 1420
AccBSI	CCGCTC	1 cut(s)	740
AciI	CCGC	9 cut(s)	81, 84, 87, 93, 218, 446, 538, 738, 1356
AclWI	GGATC	2 cut(s)	540, 1204
AcsI	RAATTY	3 cut(s)	763, 837, 1452
AcuI	CTGAAG	2 cut(s)	728, 1062
AcyI	GRCGYC	1 cut(s)	1057
AfaI	GTAC	4 cut(s)	284, 900, 946, 1422
AfiI	CCNNNNNNNGG	1 cut(s)	757
AgsI	TTSAA	6 cut(s)	300, 616, 854, 1139, 1457, 1468
Alw21I	GWGCWC	1 cut(s)	1355
Alw26I	GTCTC	5 cut(s)	196, 677, 696, 1324, 1421
AlwI	GGATC	2 cut(s)	540, 1204
Ama87I	CYCGRG	1 cut(s)	638
AoxI	GGCC	2 cut(s)	265, 361
ApeKI	GCWGC	2 cut(s)	90, 99
ApoI	RAATTY	3 cut(s)	763, 837, 1452
Asp700I	GAANNNNTTC	1 cut(s)	1452
Asp718I	GGTACC	1 cut(s)	1420
AspLEI	GCGC	1 cut(s)	750
AspS9I	GGNCC	1 cut(s)	265
AsuHPI	GGTGA	2 cut(s)	149, 225
AsuNHI	GCTAGC	1 cut(s)	412
AvaI	CYCGRG	1 cut(s)	638
AxyI	CCTNAGG	1 cut(s)	752
BanI	GGYRCC	2 cut(s)	499, 1420
BanII	GRGCYC	1 cut(s)	1355
BbsI	GAAGAC	1 cut(s)	463
Bbv12I	GWGCWC	1 cut(s)	1355
BbvI	GCAGC	2 cut(s)	86, 102
BccI	CCATC	3 cut(s)	402, 693, 1033
BceAI	ACGGC	2 cut(s)	836, 1070
BcgI	CGANNNNNNTGC	2 cut(s)	517, 551
BcoDI	GTCTC	5 cut(s)	196, 677, 696, 1324, 1421
BfaI	CTAG	4 cut(s)	120, 413, 696, 1380
BfmI	CTRYAG	3 cut(s)	100, 273, 987
BfoI	RGCGCY	1 cut(s)	751
BisI	GCNGC	6 cut(s)	82, 85, 88, 91, 100, 738
BlsI	GCNGC	6 cut(s)	83, 86, 89, 92, 101, 739
BmcAI	AGTACT	2 cut(s)	284, 946
BmeT110I	CYCGRG	1 cut(s)	638
BmgT120I	GGNCC	1 cut(s)	265
BmiI	GGNNCC	4 cut(s)	199, 501, 1011, 1422
BmtI	GCTAGC	1 cut(s)	416
BpiI	GAAGAC	1 cut(s)	463
Bpu10I	CCTNAGC	1 cut(s)	1093
BpuEI	CTTGAG	1 cut(s)	762
BsaHI	GRCGYC	1 cut(s)	1057
BsaI	GGTCTC	1 cut(s)	677
BsaJI	CCNNGG	2 cut(s)	637, 1226
BsaWI	WCCGGW	1 cut(s)	1423
BsaXI	ACNNNNNCTCC	2 cut(s)	318, 348
Bsc4I	CCNNNNNNNGG	1 cut(s)	757
Bse118I	RCCGGY	2 cut(s)	235, 1274
Bse1I	ACTGG	2 cut(s)	623, 1198
Bse21I	CCTNAGG	1 cut(s)	752
Bse3DI	GCAATG	3 cut(s)	324, 381, 927
BseDI	CCNNGG	2 cut(s)	637, 1226
BseGI	GGATG	2 cut(s)	413, 476
BseLI	CCNNNNNNNGG	1 cut(s)	757
BseMI	GCAATG	3 cut(s)	324, 381, 927
BseMII	CTCAG	2 cut(s)	293, 780
BseNI	ACTGG	2 cut(s)	623, 1198
BseRI	GAGGAG	1 cut(s)	626
BseXI	GCAGC	2 cut(s)	86, 102
BshFI	GGCC	2 cut(s)	267, 363
BshNI	GGYRCC	2 cut(s)	499, 1420
BsiHKAI	GWGCWC	1 cut(s)	1355
BsiHKCI	CYCGRG	1 cut(s)	638
BsiSI	CCGG	3 cut(s)	236, 1275, 1424
BslFI	GGGAC	2 cut(s)	655, 997
BslI	CCNNNNNNNGG	1 cut(s)	757
BsmAI	GTCTC	5 cut(s)	196, 677, 696, 1324, 1421
BsmFI	GGGAC	2 cut(s)	655, 997
BsmI	GAATGC	1 cut(s)	1221
BsnI	GGCC	2 cut(s)	267, 363
Bso31I	GGTCTC	1 cut(s)	677
BsoBI	CYCGRG	1 cut(s)	638
Bsp1286I	GDGCHC	1 cut(s)	1355
Bsp143I	GATC	4 cut(s)	259, 532, 711, 1196
BspACI	CCGC	9 cut(s)	81, 84, 87, 93, 218, 446, 538, 738, 1356
BspANI	GGCC	2 cut(s)	267, 363
BspCNI	CTCAG	2 cut(s)	292, 781
BspHI	TCATGA	1 cut(s)	252
BspLI	GGNNCC	4 cut(s)	199, 501, 1011, 1422
BspMAI	CTGCAG	1 cut(s)	104
BspOI	GCTAGC	1 cut(s)	416
BspPI	GGATC	2 cut(s)	540, 1204
BspQI	GCTCTTC	1 cut(s)	747
BspT107I	GGYRCC	2 cut(s)	499, 1420
BspTNI	GGTCTC	1 cut(s)	677
BsrBI	CCGCTC	1 cut(s)	740
BsrDI	GCAATG	3 cut(s)	324, 381, 927
BsrFI	RCCGGY	2 cut(s)	235, 1274
BsrI	ACTGG	2 cut(s)	623, 1198
BssAI	RCCGGY	2 cut(s)	235, 1274
BssECI	CCNNGG	2 cut(s)	637, 1226
BssMI	GATC	4 cut(s)	259, 532, 711, 1196
BssNI	GRCGYC	1 cut(s)	1057
BssT1I	CCWWGG	1 cut(s)	1226
Bst4CI	ACNGT	6 cut(s)	113, 274, 462, 556, 1143, 1399
Bst6I	CTCTTC	4 cut(s)	361, 680, 747, 1068
BstACI	GRCGYC	1 cut(s)	1057
BstC8I	GCNNGC	3 cut(s)	414, 624, 705
BstDEI	CTNAG	6 cut(s)	66, 279, 429, 752, 789, 1093
BstF5I	GGATG	2 cut(s)	413, 476
BstH2I	RGCGCY	1 cut(s)	751
BstHHI	GCGC	1 cut(s)	750
BstKTI	GATC	4 cut(s)	262, 535, 714, 1199
BstMAI	GTCTC	5 cut(s)	196, 677, 696, 1324, 1421
BstMBI	GATC	4 cut(s)	259, 532, 711, 1196
BstNSI	RCATGY	1 cut(s)	1092
BstSFI	CTRYAG	3 cut(s)	100, 273, 987
BstV1I	GCAGC	2 cut(s)	86, 102
BstV2I	GAAGAC	1 cut(s)	463
BstX2I	RGATCY	1 cut(s)	532
BstXI	CCANNNNNNTGG	1 cut(s)	163
BstYI	RGATCY	1 cut(s)	532
Bsu36I	CCTNAGG	1 cut(s)	752
BsuRI	GGCC	2 cut(s)	267, 363
BtsCI	GGATG	2 cut(s)	413, 476
BtsI	GCAGTG	1 cut(s)	109
BtsIMutI	CAGTG	3 cut(s)	109, 270, 1148
Cac8I	GCNNGC	3 cut(s)	414, 624, 705
CciI	TCATGA	1 cut(s)	252
CfoI	GCGC	1 cut(s)	750
Cfr10I	RCCGGY	2 cut(s)	235, 1274
Cfr13I	GGNCC	1 cut(s)	265
CseI	GACGC	1 cut(s)	1046
Csp6I	GTAC	4 cut(s)	283, 899, 945, 1421
CviAII	CATG	4 cut(s)	253, 1089, 1213, 1261
CviQI	GTAC	4 cut(s)	283, 899, 945, 1421
DdeI	CTNAG	6 cut(s)	66, 279, 429, 752, 789, 1093
DpnI	GATC	4 cut(s)	261, 534, 713, 1198
DpnII	GATC	4 cut(s)	259, 532, 711, 1196
Eam1104I	CTCTTC	4 cut(s)	361, 680, 747, 1068
EarI	CTCTTC	4 cut(s)	361, 680, 747, 1068
EciI	GGCGGA	1 cut(s)	461
Ecl136II	GAGCTC	1 cut(s)	1353
Eco130I	CCWWGG	1 cut(s)	1226
Eco24I	GRGCYC	1 cut(s)	1355
Eco31I	GGTCTC	1 cut(s)	677
Eco53kI	GAGCTC	1 cut(s)	1353
Eco57I	CTGAAG	2 cut(s)	728, 1062
Eco81I	CCTNAGG	1 cut(s)	752
Eco88I	CYCGRG	1 cut(s)	638
EcoICRI	GAGCTC	1 cut(s)	1353
EcoRI	GAATTC	2 cut(s)	763, 837
EcoT14I	CCWWGG	1 cut(s)	1226
EcoT38I	GRGCYC	1 cut(s)	1355
ErhI	CCWWGG	1 cut(s)	1226
FaeI	CATG	4 cut(s)	256, 1092, 1216, 1264
FalI	AAGNNNNNCTT	2 cut(s)	726, 758
FaqI	GGGAC	2 cut(s)	655, 997
FatI	CATG	4 cut(s)	252, 1088, 1212, 1260
FauI	CCCGC	1 cut(s)	531
Fnu4HI	GCNGC	6 cut(s)	82, 85, 88, 91, 100, 738
FokI	GGATG	2 cut(s)	420, 483
FriOI	GRGCYC	1 cut(s)	1355
Fsp4HI	GCNGC	6 cut(s)	82, 85, 88, 91, 100, 738
FspBI	CTAG	4 cut(s)	120, 413, 696, 1380
GlaI	GCGC	1 cut(s)	749
GluI	GCNGC	6 cut(s)	82, 85, 88, 91, 100, 738
HaeII	RGCGCY	1 cut(s)	751
HaeIII	GGCC	2 cut(s)	267, 363
HapII	CCGG	3 cut(s)	236, 1275, 1424
HgaI	GACGC	1 cut(s)	1046
HhaI	GCGC	1 cut(s)	750
Hin1I	GRCGYC	1 cut(s)	1057
Hin1II	CATG	4 cut(s)	256, 1092, 1216, 1264
Hin6I	GCGC	1 cut(s)	748
HinP1I	GCGC	1 cut(s)	748
HinfI	GANTC	1 cut(s)	689
HpaII	CCGG	3 cut(s)	236, 1275, 1424
HphI	GGTGA	2 cut(s)	149, 225
Hpy188I	TCNGA	8 cut(s)	314, 388, 816, 829, 914, 973, 1243, 1480
Hpy188III	TCNNGA	5 cut(s)	253, 640, 686, 779, 842
HpyAV	CCTTC	6 cut(s)	17, 125, 523, 943, 1145, 1258
HpyCH4III	ACNGT	6 cut(s)	113, 274, 462, 556, 1143, 1399
HpyCH4V	TGCA	7 cut(s)	102, 374, 399, 941, 1001, 1129, 1412
HpyF3I	CTNAG	6 cut(s)	66, 279, 429, 752, 789, 1093
Hsp92I	GRCGYC	1 cut(s)	1057
Hsp92II	CATG	4 cut(s)	256, 1092, 1216, 1264
HspAI	GCGC	1 cut(s)	748
KpnI	GGTACC	1 cut(s)	1424
Kzo9I	GATC	4 cut(s)	259, 532, 711, 1196
LguI	GCTCTTC	1 cut(s)	747
LmnI	GCTCC	6 cut(s)	75, 203, 314, 326, 1350, 1358
Lsp1109I	GCAGC	2 cut(s)	86, 102
MaeI	CTAG	4 cut(s)	120, 413, 696, 1380
MaeIII	GTNAC	1 cut(s)	1393
MalI	GATC	4 cut(s)	261, 534, 713, 1198
MbiI	CCGCTC	1 cut(s)	740
MboI	GATC	4 cut(s)	259, 532, 711, 1196
MflI	RGATCY	1 cut(s)	532
MhlI	GDGCHC	1 cut(s)	1355
MluCI	AATT	7 cut(s)	763, 837, 908, 1032, 1333, 1452, 1483
MlyI	GAGTC	1 cut(s)	698
MmeI	TCCRAC	1 cut(s)	448
MroXI	GAANNNNTTC	1 cut(s)	1452
MseI	TTAA	1 cut(s)	1332
MslI	CAYNNNNRTG	2 cut(s)	161, 1259
MspA1I	CMGCKG	2 cut(s)	93, 962
MspI	CCGG	3 cut(s)	236, 1275, 1424
Mva1269I	GAATGC	1 cut(s)	1221
NdeII	GATC	4 cut(s)	259, 532, 711, 1196
NheI	GCTAGC	1 cut(s)	412
NlaIII	CATG	4 cut(s)	256, 1092, 1216, 1264
NlaIV	GGNNCC	4 cut(s)	199, 501, 1011, 1422
NspI	RCATGY	1 cut(s)	1092
PagI	TCATGA	1 cut(s)	252
PciSI	GCTCTTC	1 cut(s)	747
PctI	GAATGC	1 cut(s)	1221
PdmI	GAANNNNTTC	1 cut(s)	1452
PflFI	GACNNNGTC	1 cut(s)	1056
PkrI	GCNGC	6 cut(s)	83, 86, 89, 92, 101, 739
PleI	GAGTC	1 cut(s)	697
PpsI	GAGTC	1 cut(s)	697
PsiI	TTATAA	1 cut(s)	1235
Psp124BI	GAGCTC	1 cut(s)	1355
PspN4I	GGNNCC	4 cut(s)	199, 501, 1011, 1422
PspPI	GGNCC	1 cut(s)	265
PstI	CTGCAG	1 cut(s)	104
PsuI	RGATCY	1 cut(s)	532
PsyI	GACNNNGTC	1 cut(s)	1056
PvuII	CAGCTG	1 cut(s)	962
RsaI	GTAC	4 cut(s)	284, 900, 946, 1422
RsaNI	GTAC	4 cut(s)	283, 899, 945, 1421
RseI	CAYNNNNRTG	2 cut(s)	161, 1259
SacI	GAGCTC	1 cut(s)	1355
SapI	GCTCTTC	1 cut(s)	747
SaqAI	TTAA	1 cut(s)	1332
SatI	GCNGC	6 cut(s)	82, 85, 88, 91, 100, 738
Sau3AI	GATC	4 cut(s)	259, 532, 711, 1196
Sau96I	GGNCC	1 cut(s)	265
ScaI	AGTACT	2 cut(s)	284, 946
SchI	GAGTC	1 cut(s)	698
SduI	GDGCHC	1 cut(s)	1355
SfcI	CTRYAG	3 cut(s)	100, 273, 987
SmiMI	CAYNNNNRTG	2 cut(s)	161, 1259
SmlI	CTYRAG	1 cut(s)	777
SmoI	CTYRAG	1 cut(s)	777
Sse9I	AATT	7 cut(s)	763, 837, 908, 1032, 1333, 1452, 1483
SsiI	CCGC	9 cut(s)	81, 84, 87, 93, 218, 446, 538, 738, 1356
SspI	AATATT	1 cut(s)	303
SspMI	CTAG	4 cut(s)	120, 413, 696, 1380
SstI	GAGCTC	1 cut(s)	1355
StyI	CCWWGG	1 cut(s)	1226
TaaI	ACNGT	6 cut(s)	113, 274, 462, 556, 1143, 1399
TaqII	GACCGA	1 cut(s)	231
TasI	AATT	7 cut(s)	763, 837, 908, 1032, 1333, 1452, 1483
TatI	WGTACW	2 cut(s)	282, 944
TauI	GCSGC	4 cut(s)	84, 87, 90, 740
Tru1I	TTAA	1 cut(s)	1332
Tru9I	TTAA	1 cut(s)	1332
TscAI	CASTG	3 cut(s)	109, 277, 1148
TseI	GCWGC	2 cut(s)	90, 99
TspDTI	ATGAA	6 cut(s)	141, 269, 548, 670, 943, 1277
TspGWI	ACGGA	1 cut(s)	639
TspRI	CASTG	3 cut(s)	109, 277, 1148
Tth111I	GACNNNGTC	1 cut(s)	1056
XapI	RAATTY	3 cut(s)	763, 837, 1452
XceI	RCATGY	1 cut(s)	1092
XmnI	GAANNNNTTC	1 cut(s)	1452
XspI	CTAG	4 cut(s)	120, 413, 696, 1380
ZrmI	AGTACT	2 cut(s)	284, 946

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.