MD12G1227100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013030
Physical Location & Seq
Forward (+)
30272865 .. 30274634
1770 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1227100.v1.1.491

Sequence Viewer

Length: 1770 bp
ATGGACGAGCCGTCCGAGAAGCTCGAGAACCTCAAGTCGCTGAACTCTCTGCTGCTCAAGGAGACCAAGGACCGCCGGCAGCAGGTGGAGTCGCTGGTGCAGGCCAAGGCGGGTTTGGAGTCCGAGCTTAGTCGGTTCCGAGTGGAGAGTGAGTCGCTTGTGAGCGAGTTGAGTGAGAAGAGCGAGGAAAATGTTGGGTTGGAGTTGGAGAAGAGTGTTTATGGTGTTTTTGTTCTGGCCCAGATGGGGCAAATGATGAGAGAGAGAGTTGAGATTGAGAGGGCGATGAGTGAGAGAGATGGTGAGATTGTGGAGTTGAAGAGGGAAGTGGAGGAGCTTGCGGGTGGACTCGAAATCGAGAAGGGGAAGCTGAGCAGAGTGTGCTGGGAGAGGGATTGTGTGAAGAGGGATTTCGGTGGCCTCTCGGAGGAGGCGAATGGGTTGAAGTTGAAAGTGGTGGAAATGGAAAAGAAGGAGAGGGTTTTCGGGGAGGAATTCGAGAAGCTGCAAGTGCATTGCAATGGGTTGGTACAGGACAAGGCTGGGAAGGAGAGGGAGGTTGAGATTGCAATGAGAGAGAAGGAGTTGGCTGAGATGAAGCGCGATGAGTCGGAGAGAGTGATCGATGATTTGAAGAGGGAGATTAAAAGGGTTGTGATGGAAAAGAATGAGATTGAGAAGGAAAAGAGCGGGCATGAAGCGAGGATTTTTCAATTGGAGAATGAAGCGGGGCAGTTGAGTAAGATCGAATTGGGTTTGAGGAAGGAGAATGCAGTGTTGCATGTGAAGGTTTTGGAGTTGGAGAAGATTGTTGAGGAGGCTATGGGGAAGGGAGAGGTGTTGGAGAGGGAGATTAAGGTTTTGGGGGAAGAGAAGAGGGAGAAAGAGCAGAGTTTCGAGAAGTTGACTGAGGAAGTGAAATCACAGAAGGAACTTTTGGATATGGTTACTGAGGAACTGAAGAAAAAGGAGGAAAGAATCAAGGAAATCGAGCTAAAGAAGAATGAGATCAAGGAGGCAAAAGTGAAGCAAGAGAAGGAAATTGCTGAGTTGACAAGTCAAGTGAAAGAAGAAAGGGATTTTGTTTCTACATTGCGAAATTCATGCAACGAGCAGAAGGAGAAGAATGCGCAGTTGGTTTCTGAAGTTAGTCGATATAAAGATGCTCTTGATCGTGTTACGGAGGAGAAGGCAGAGGCTCTGAAGAATTTGGATGGGGAGAAGAAGAAAGTGGAAGACTTAATGCTGACCATTTCGGGGAGGGAGAAGACAATCAAAGAAATCGAGAAAGAGTTGGAGAAACTGAGGAGTGTGCAAGAGAACGTTGCTGAGAAAAATAAGGCCATGGAGAGCCGATTGGAAACATTGGTGAAGGAAAAAGATGTGCTGCAGAAGAACCTTGTTGAGGCTCAGAGGAAAATTCGTGATTGGGAGGCTAAATTTGAGTCAGAAGGAACCAAAATGGAGCTTGCTTTGACTCTGCTGAAGAACACTGCAGCACGTGTATCCTTGAAGTCCGAAGGCAAGGAAGAAGTGGCTACCAATGATCTCAAGGTTGGCGAAGAAATTAAGCCATATGCTGCGGAGTTGGATGCCATACAGACTGCTTTCAGAAACAAGGAGAAGATGGTTGGAGACTTGCAGAAACAACTCGAGTCTATGCAGAAGTCATCCGAGGCACAGAAGAAGAAGAGTTTCTGGACCTTGATCTCATCAGCGACAACCCTTATTGCTGCAGCATCTTTTGCATATGTTTCCAAAGCGCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04530 map04530
Pfam Domains
Protein Families
No domains found.

Protein Analysis

590

Amino Acids

67.8

Weight (kDa)

5.32

Isoelectric Point (pI)

42.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013030)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05130 AT5G27330
fragaria_vesca FvH4_6g04961
malus_domestica MD04G1212300.v1.1 MD12G1227100.v1.1
prunus_persica Prupe.6G332700_v2.0.a1
pyrus_communis pycom04g18840 pycom12g21180
rosa_chinensis RchiOBHm_Chr3g0453461
rosa_laevigata RLG00000025512
rosa_multiflora Rmu_sc0000510.1_g000009
rosa_roxburghii Rroxscaffold_6G00430430
rosa_rugosa Rorug02G0649200
rosa_samantha Rh3AG052000 Rh3BG053500 Rh3CG052800 Rh3DG053600
rosa_wichuraiana Rw3G004010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 73
Acc16I TGCGCA 1 cut(s) 1131
Acc36I ACCTGC 1 cut(s) 73
AccBSI CCGCTC 1 cut(s) 690
AccII CGCG 2 cut(s) 603, 1765
AciI CCGC 6 cut(s) 73, 110, 341, 690, 728, 1583
AclI AACGTT 1 cut(s) 1323
AcsI RAATTY 5 cut(s) 494, 1099, 1207, 1419, 1439
AcuI CTGAAG 4 cut(s) 980, 1164, 1223, 1505
AcvI CACGTG 1 cut(s) 1502
AfaI GTAC 1 cut(s) 531
AfiI CCNNNNNNNGG 5 cut(s) 82, 246, 427, 1257, 1405
AflIII ACRYGT 1 cut(s) 1501
AgsI TTSAA 6 cut(s) 319, 445, 451, 634, 713, 1513
AhdI GACNNNNNGTC 1 cut(s) 10
AjuI GAANNNNNNNTTGG 4 cut(s) 920, 952, 1118, 1150
AluBI AGCT 7 cut(s) 22, 127, 337, 370, 505, 994, 1468
AluI AGCT 7 cut(s) 22, 127, 337, 370, 505, 994, 1468
Alw26I GTCTC 2 cut(s) 56, 1629
Ama87I CYCGRG 2 cut(s) 23, 1652
AoxI GGCC 4 cut(s) 102, 237, 418, 1341
ApeKI GCWGC 8 cut(s) 52, 79, 505, 1387, 1496, 1580, 1733, 1736
ApoI RAATTY 5 cut(s) 494, 1099, 1207, 1419, 1439
Asp700I GAANNNNTTC 1 cut(s) 1694
AspLEI GCGC 4 cut(s) 603, 1132, 1765, 1767
AspS9I GGNCC 3 cut(s) 70, 238, 1701
AsuHPI GGTGA 2 cut(s) 314, 1381
AvaI CYCGRG 2 cut(s) 23, 1652
AvaII GGWCC 2 cut(s) 70, 1701
BbrPI CACGTG 1 cut(s) 1502
BbsI GAAGAC 2 cut(s) 1242, 1274
BbvI GCAGC 8 cut(s) 39, 91, 492, 1374, 1508, 1567, 1720, 1748
BccI CCATC 5 cut(s) 238, 293, 652, 1208, 1621
BcgI CGANNNNNNTGC 2 cut(s) 1086, 1120
BciVI GTATCC 1 cut(s) 1516
BcoDI GTCTC 2 cut(s) 56, 1629
BfmI CTRYAG 3 cut(s) 1388, 1494, 1734
BfuAI ACCTGC 1 cut(s) 73
BfuI GTATCC 1 cut(s) 1516
BisI GCNGC 8 cut(s) 53, 80, 506, 1388, 1497, 1581, 1734, 1737
BlpI GCTNAGC 1 cut(s) 371
BlsI GCNGC 8 cut(s) 54, 81, 507, 1389, 1498, 1582, 1735, 1738
Bme18I GGWCC 2 cut(s) 70, 1701
BmeRI GACNNNNNGTC 1 cut(s) 10
BmeT110I CYCGRG 2 cut(s) 23, 1652
BmgT120I GGNCC 3 cut(s) 70, 238, 1701
BmiI GGNNCC 2 cut(s) 137, 1456
BmsI GCATC 3 cut(s) 1153, 1582, 1748
BpiI GAAGAC 2 cut(s) 1242, 1274
Bpu1102I GCTNAGC 1 cut(s) 371
BpuEI CTTGAG 3 cut(s) 17, 41, 1535
Bsa29I ATCGAT 1 cut(s) 624
BsaAI YACGTR 1 cut(s) 1502
BsaI GGTCTC 1 cut(s) 56
BsaJI CCNNGG 4 cut(s) 66, 105, 1344, 1674
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 5 cut(s) 82, 246, 427, 1257, 1405
Bse118I RCCGGY 1 cut(s) 75
Bse3DI GCAATG 4 cut(s) 514, 526, 576, 1091
BseCI ATCGAT 1 cut(s) 624
BseDI CCNNGG 4 cut(s) 66, 105, 1344, 1674
BseGI GGATG 3 cut(s) 1219, 1597, 1670
BseLI CCNNNNNNNGG 5 cut(s) 82, 246, 427, 1257, 1405
BseMI GCAATG 4 cut(s) 514, 526, 576, 1091
BseMII CTCAG 8 cut(s) 362, 582, 900, 942, 1038, 1295, 1320, 1424
BsePI GCGCGC 1 cut(s) 1763
BseRI GAGGAG 5 cut(s) 347, 443, 830, 1199, 1321
BseXI GCAGC 8 cut(s) 39, 91, 492, 1374, 1508, 1567, 1720, 1748
BseYI CCCAGC 2 cut(s) 384, 542
BsgI GTGCAG 1 cut(s) 119
Bsh1236I CGCG 2 cut(s) 603, 1765
BshFI GGCC 4 cut(s) 104, 239, 420, 1343
BshVI ATCGAT 1 cut(s) 624
BsiHKCI CYCGRG 2 cut(s) 23, 1652
BsiSI CCGG 1 cut(s) 76
BslI CCNNNNNNNGG 5 cut(s) 82, 246, 427, 1257, 1405
BsmAI GTCTC 2 cut(s) 56, 1629
BsmI GAATGC 2 cut(s) 775, 1132
BsnI GGCC 4 cut(s) 104, 239, 420, 1343
Bso31I GGTCTC 1 cut(s) 56
BsoBI CYCGRG 2 cut(s) 23, 1652
Bsp143I GATC 6 cut(s) 621, 744, 1008, 1171, 1546, 1707
Bsp1720I GCTNAGC 1 cut(s) 371
Bsp19I CCATGG 1 cut(s) 1344
BspACI CCGC 6 cut(s) 73, 110, 341, 690, 728, 1583
BspANI GGCC 4 cut(s) 104, 239, 420, 1343
BspCNI CTCAG 8 cut(s) 363, 583, 901, 943, 1039, 1296, 1321, 1423
BspDI ATCGAT 1 cut(s) 624
BspFNI CGCG 2 cut(s) 603, 1765
BspLI GGNNCC 2 cut(s) 137, 1456
BspMAI CTGCAG 3 cut(s) 1392, 1498, 1738
BspMI ACCTGC 1 cut(s) 73
BspQI GCTCTTC 1 cut(s) 173
BspTNI GGTCTC 1 cut(s) 56
BsrBI CCGCTC 1 cut(s) 690
BsrDI GCAATG 4 cut(s) 514, 526, 576, 1091
BsrFI RCCGGY 1 cut(s) 75
BssAI RCCGGY 1 cut(s) 75
BssECI CCNNGG 4 cut(s) 66, 105, 1344, 1674
BssHII GCGCGC 1 cut(s) 1763
BssMI GATC 6 cut(s) 621, 744, 1008, 1171, 1546, 1707
BssT1I CCWWGG 3 cut(s) 66, 105, 1344
Bst6I CTCTTC 8 cut(s) 173, 206, 314, 398, 629, 864, 869, 1685
BstAPI GCANNNNNTGC 2 cut(s) 381, 1745
BstBAI YACGTR 1 cut(s) 1502
BstC8I GCNNGC 6 cut(s) 77, 102, 339, 692, 1470, 1765
BstDEI CTNAG 9 cut(s) 128, 371, 591, 909, 951, 1047, 1304, 1329, 1410
BstDSI CCRYGG 1 cut(s) 1344
BstENI CCTNNNNNAGG 2 cut(s) 425, 1403
BstF5I GGATG 3 cut(s) 1219, 1597, 1670
BstFNI CGCG 2 cut(s) 603, 1765
BstHHI GCGC 4 cut(s) 603, 1132, 1765, 1767
BstKTI GATC 6 cut(s) 624, 747, 1011, 1174, 1549, 1710
BstMAI GTCTC 2 cut(s) 56, 1629
BstMBI GATC 6 cut(s) 621, 744, 1008, 1171, 1546, 1707
BstMWI GCNNNNNNNGC 3 cut(s) 381, 511, 1745
BstNSI RCATGY 1 cut(s) 785
BstSFI CTRYAG 3 cut(s) 1388, 1494, 1734
BstUI CGCG 2 cut(s) 603, 1765
BstV1I GCAGC 8 cut(s) 39, 91, 492, 1374, 1508, 1567, 1720, 1748
BstV2I GAAGAC 2 cut(s) 1242, 1274
Bsu15I ATCGAT 1 cut(s) 624
BsuI GTATCC 1 cut(s) 1516
BsuRI GGCC 4 cut(s) 104, 239, 420, 1343
BsuTUI ATCGAT 1 cut(s) 624
BtgI CCRYGG 1 cut(s) 1344
BtgZI GCGATG 2 cut(s) 299, 618
BtsCI GGATG 3 cut(s) 1219, 1597, 1670
BtsI GCAGTG 2 cut(s) 780, 1491
BtsIMutI CAGTG 2 cut(s) 780, 1491
BveI ACCTGC 1 cut(s) 73
Cac8I GCNNGC 6 cut(s) 77, 102, 339, 692, 1470, 1765
CfoI GCGC 4 cut(s) 603, 1132, 1765, 1767
Cfr10I RCCGGY 1 cut(s) 75
Cfr13I GGNCC 3 cut(s) 70, 238, 1701
ClaI ATCGAT 1 cut(s) 624
Csp6I GTAC 1 cut(s) 530
CviAII CATG 4 cut(s) 695, 782, 1104, 1345
CviQI GTAC 1 cut(s) 530
DdeI CTNAG 9 cut(s) 128, 371, 591, 909, 951, 1047, 1304, 1329, 1410
DpnI GATC 6 cut(s) 623, 746, 1010, 1173, 1548, 1709
DpnII GATC 6 cut(s) 621, 744, 1008, 1171, 1546, 1707
DriI GACNNNNNGTC 1 cut(s) 10
Eam1104I CTCTTC 8 cut(s) 173, 206, 314, 398, 629, 864, 869, 1685
Eam1105I GACNNNNNGTC 1 cut(s) 10
EarI CTCTTC 8 cut(s) 173, 206, 314, 398, 629, 864, 869, 1685
Eco130I CCWWGG 3 cut(s) 66, 105, 1344
Eco31I GGTCTC 1 cut(s) 56
Eco47I GGWCC 2 cut(s) 70, 1701
Eco57I CTGAAG 4 cut(s) 980, 1164, 1223, 1505
Eco72I CACGTG 1 cut(s) 1502
Eco88I CYCGRG 2 cut(s) 23, 1652
EcoNI CCTNNNNNAGG 2 cut(s) 425, 1403
EcoRI GAATTC 1 cut(s) 494
EcoT14I CCWWGG 3 cut(s) 66, 105, 1344
ErhI CCWWGG 3 cut(s) 66, 105, 1344
FaeI CATG 4 cut(s) 698, 785, 1107, 1348
FalI AAGNNNNNCTT 2 cut(s) 1152, 1184
FatI CATG 4 cut(s) 694, 781, 1103, 1344
FauI CCCGC 4 cut(s) 103, 334, 683, 721
FauNDI CATATG 2 cut(s) 1576, 1750
Fnu4HI GCNGC 8 cut(s) 53, 80, 506, 1388, 1497, 1581, 1734, 1737
FokI GGATG 3 cut(s) 1226, 1604, 1657
Fsp4HI GCNGC 8 cut(s) 53, 80, 506, 1388, 1497, 1581, 1734, 1737
FspI TGCGCA 1 cut(s) 1131
GlaI GCGC 4 cut(s) 602, 1131, 1764, 1766
GluI GCNGC 8 cut(s) 53, 80, 506, 1388, 1497, 1581, 1734, 1737
GsaI CCCAGC 2 cut(s) 388, 546
HaeIII GGCC 4 cut(s) 104, 239, 420, 1343
HapII CCGG 1 cut(s) 76
HhaI GCGC 4 cut(s) 603, 1132, 1765, 1767
Hin1II CATG 4 cut(s) 698, 785, 1107, 1348
Hin6I GCGC 4 cut(s) 601, 1130, 1763, 1765
HinP1I GCGC 4 cut(s) 601, 1130, 1763, 1765
HincII GTYRAC 2 cut(s) 906, 1053
HindII GTYRAC 2 cut(s) 906, 1053
HinfI GANTC 9 cut(s) 89, 119, 152, 348, 608, 978, 1445, 1477, 1655
HpaII CCGG 1 cut(s) 76
HphI GGTGA 2 cut(s) 314, 1381
Hpy166II GTNNAC 3 cut(s) 347, 906, 1053
Hpy188III TCNNGA 8 cut(s) 25, 358, 499, 898, 1169, 1285, 1424, 1699
Hpy8I GTNNAC 3 cut(s) 347, 906, 1053
HpyCH4IV ACGT 2 cut(s) 1323, 1501
HpyF10VI GCNNNNNNNGC 3 cut(s) 381, 511, 1745
HpyF3I CTNAG 9 cut(s) 128, 371, 591, 909, 951, 1047, 1304, 1329, 1410
HpySE526I ACGT 2 cut(s) 1323, 1501
Hsp92II CATG 4 cut(s) 698, 785, 1107, 1348
HspAI GCGC 4 cut(s) 601, 1130, 1763, 1765
KroI GCCGGC 1 cut(s) 75
KroNI GCCGGC 1 cut(s) 77
Kzo9I GATC 6 cut(s) 621, 744, 1008, 1171, 1546, 1707
LguI GCTCTTC 1 cut(s) 173
LmnI GCTCC 2 cut(s) 334, 1465
Lsp1109I GCAGC 8 cut(s) 39, 91, 492, 1374, 1508, 1567, 1720, 1748
LweI GCATC 3 cut(s) 1153, 1582, 1748
MaeII ACGT 2 cut(s) 1323, 1501
MaeIII GTNAC 2 cut(s) 946, 1177
MalI GATC 6 cut(s) 623, 746, 1010, 1173, 1548, 1709
MbiI CCGCTC 1 cut(s) 690
MboI GATC 6 cut(s) 621, 744, 1008, 1171, 1546, 1707
MfeI CAATTG 1 cut(s) 713
MluCI AATT 9 cut(s) 494, 713, 749, 1041, 1099, 1207, 1419, 1439, 1566
MlyI GAGTC 8 cut(s) 98, 128, 161, 342, 617, 1454, 1471, 1664
MmeI TCCRAC 8 cut(s) 180, 186, 591, 780, 822, 1275, 1569, 1612
MroNI GCCGGC 1 cut(s) 75
MroXI GAANNNNTTC 1 cut(s) 1694
MseI TTAA 4 cut(s) 645, 855, 1241, 1569
MslI CAYNNNNRTG 1 cut(s) 519
MspI CCGG 1 cut(s) 76
MunI CAATTG 1 cut(s) 713
Mva1269I GAATGC 2 cut(s) 775, 1132
MvnI CGCG 2 cut(s) 603, 1765
MwoI GCNNNNNNNGC 3 cut(s) 381, 511, 1745
NaeI GCCGGC 1 cut(s) 77
NcoI CCATGG 1 cut(s) 1344
NdeI CATATG 2 cut(s) 1576, 1750
NdeII GATC 6 cut(s) 621, 744, 1008, 1171, 1546, 1707
NgoMIV GCCGGC 1 cut(s) 75
NlaIII CATG 4 cut(s) 698, 785, 1107, 1348
NlaIV GGNNCC 2 cut(s) 137, 1456
NsbI TGCGCA 1 cut(s) 1131
NspI RCATGY 1 cut(s) 785
PaeR7I CTCGAG 2 cut(s) 23, 1652
PaqCI CACCTGC 1 cut(s) 73
PauI GCGCGC 1 cut(s) 1763
PciSI GCTCTTC 1 cut(s) 173
PcsI WCGNNNNNNNCGW 2 cut(s) 12, 431
PctI GAATGC 2 cut(s) 775, 1132
PdiI GCCGGC 1 cut(s) 77
PdmI GAANNNNTTC 1 cut(s) 1694
PfeI GAWTC 1 cut(s) 978
PkrI GCNGC 8 cut(s) 54, 81, 507, 1389, 1498, 1582, 1735, 1738
PleI GAGTC 8 cut(s) 97, 127, 160, 342, 616, 1453, 1471, 1663
PmaCI CACGTG 1 cut(s) 1502
PmlI CACGTG 1 cut(s) 1502
PpsI GAGTC 8 cut(s) 97, 127, 160, 342, 616, 1453, 1471, 1663
Ppu21I YACGTR 1 cut(s) 1502
Psp1406I AACGTT 1 cut(s) 1323
PspCI CACGTG 1 cut(s) 1502
PspFI CCCAGC 2 cut(s) 384, 542
PspN4I GGNNCC 2 cut(s) 137, 1456
PspPI GGNCC 3 cut(s) 70, 238, 1701
PspXI VCTCGAGB 1 cut(s) 1652
PstI CTGCAG 3 cut(s) 1392, 1498, 1738
PteI GCGCGC 1 cut(s) 1763
RsaI GTAC 1 cut(s) 531
RsaNI GTAC 1 cut(s) 530
RseI CAYNNNNRTG 1 cut(s) 519
SapI GCTCTTC 1 cut(s) 173
SaqAI TTAA 4 cut(s) 645, 855, 1241, 1569
SatI GCNGC 8 cut(s) 53, 80, 506, 1388, 1497, 1581, 1734, 1737
Sau3AI GATC 6 cut(s) 621, 744, 1008, 1171, 1546, 1707
Sau96I GGNCC 3 cut(s) 70, 238, 1701
SchI GAGTC 8 cut(s) 98, 128, 161, 342, 617, 1454, 1471, 1664
SfaNI GCATC 3 cut(s) 1153, 1582, 1748
SfcI CTRYAG 3 cut(s) 1388, 1494, 1734
Sfr274I CTCGAG 2 cut(s) 23, 1652
SinI GGWCC 2 cut(s) 70, 1701
SlaI CTCGAG 2 cut(s) 23, 1652
SmiMI CAYNNNNRTG 1 cut(s) 519
SmlI CTYRAG 5 cut(s) 23, 32, 56, 1550, 1652
SmoI CTYRAG 5 cut(s) 23, 32, 56, 1550, 1652
Sse9I AATT 9 cut(s) 494, 713, 749, 1041, 1099, 1207, 1419, 1439, 1566
SsiI CCGC 6 cut(s) 73, 110, 341, 690, 728, 1583
StyI CCWWGG 3 cut(s) 66, 105, 1344
TaiI ACGT 2 cut(s) 1326, 1504
TasI AATT 9 cut(s) 494, 713, 749, 1041, 1099, 1207, 1419, 1439, 1566
TfiI GAWTC 1 cut(s) 978
Tru1I TTAA 4 cut(s) 645, 855, 1241, 1569
Tru9I TTAA 4 cut(s) 645, 855, 1241, 1569
TscAI CASTG 2 cut(s) 780, 1498
TseI GCWGC 8 cut(s) 52, 79, 505, 1387, 1496, 1580, 1733, 1736
TspDTI ATGAA 4 cut(s) 611, 711, 738, 1092
TspGWI ACGGA 1 cut(s) 1196
TspRI CASTG 2 cut(s) 780, 1498
VpaK11BI GGWCC 2 cut(s) 70, 1701
XagI CCTNNNNNAGG 2 cut(s) 425, 1403
XapI RAATTY 5 cut(s) 494, 1099, 1207, 1419, 1439
XceI RCATGY 1 cut(s) 785
XcmI CCANNNNNNNNNTGG 1 cut(s) 112
XhoI CTCGAG 2 cut(s) 23, 1652
XmnI GAANNNNTTC 1 cut(s) 1694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.