Rh3AG052000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0013030
Physical Location & Seq
Reverse (-)
3655444 .. 3658975
3532 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG052000.1

Sequence Viewer

Length: 1887 bp
ATGGCCAAAAAGAAAGCGACCCATCAACCCAAAGACGCCACTAGTCAAGAAGAAGAAGAAGCAACCCAGCAGACCCAGAACCACAACAACAACACCACCGCCGCCGCCGCCTCCATGGGCGACGCGTCGTCGGAGAAGCTCGCCAACCTCAAGTCGCTGAACTCTCTGCTTCTCAAGGAGACCCTCGATCGGAGGCAGCAGGTGGAGGCTCTGGTCAAGGCCCAGAAGGGTTTGGAGTCTGAGCTGAGTAAGTTGGGGATGGAGAAGAAGCTCATGGAGGTTGAGCTGGTGGAGAAGAGTGACGAGGGGTTTGGTGTCGAGTTGGAGAAGAGTGTTTTCGGGGTTTTCGTGGCGGCCCAGATGGCTGAAATGCTGAGAGAGAAGGCTGAGATTGAGAGGGTGAAGAGGGAAGCAGAGGAGGAGGTTGAGGTTTTGAGGAGGGAGGTGGATGAGCTTGTGGGGAGTCTTGAAAATGAGAAGGGTAAGCTGAGCCGGGTCTGTTGTGAGAGAGATGTGGTGAAGGGGGATTTCGACGGCCTTGCTCTGGAGGCGAATGGGTTGAGGTTGAAGGTGGTGGAGATGGAGAGGAGAGAGAGGTGTGTGGAGGATGAAGTGGAGAAGGTGAAGTCGCAATGCCGAGGGTTGGTGGAGGAGAAATGTGAGAAGGAGAGAGTGGTTGAGGCGATGATGAGGGAGAGGGATTTGGCGGAGAGGAAGCGTGGTGAGTTGGAGAGTGTGATTGCGGATTTGGAGAGGGAGATTGGGAGGATTGTCAGGGAGAAGAATGAGATTGATAAGGCGAAGATTGGGCAGGAAGTGATGGTTTCTAAGTTGGAGAAAGAGGTCGGGCAATTGAATAAGGTTGGATTGAGTTTGAGGACGGAGAACGAGGGGTTGCAGAAGAAGGTTTCGGAGCTGGAGGAGAGGGTTGGTGAGGCTGCGGCAAAGGAAAGGGAGATGGAGAGGGAGATTAAGGCCTTGGTGGAAGCGAAGAAGGAGAAGGAGGATAGTGTTGAGAGATTGAATGAGGAAGTGAAATCGCAGAAGGCAATTTTGGATATGGTCACTGAGGAATTGGAGAACAAGGAGCAGAGAATCAAGGAAATAACTCAAAAGAAGAATGAGATTGAGGAGGCAAAGGCCAATCGAGATAGTGAAGTTGCTGAGCTGAGTAGACAAGTGGGTGAGCAAAGGGATGTTATATTTACTCTGCGAAAATCGTGCAATGAGAAAGAAGAGAAGAATGCACGGTTGGTTTCTGAAGTTTGTCAATATAAAGATGCTGTTGAGCGTGTTAAGCAAGAGAGGGCTGAGGTTCAGAAAAGTTTGGCTGAGCAGAAAAAGATAGTGGAGGATTTGAAGCTGATTGTTTCCGAAAGGGAGAAGAAGGTTCAGGAAATAGAGCAATTGTTGGGGAAGCTGAGGACTGAGAGCGATGACATTACTGAGAAGAATAAAGTGATGGAGAGCCGCTTAGAATCATTGGCAAAGGAAAAGGATGTTGTGCAGAAGAGCCTTTCTGATGCTCAGAGGGATATTCATGATTGGAAGGTTAAATATGAATCGGCAGGGAGGACCTCGAAACAAGCATTGACTATGTTGAAGAACACTGCAGCATTCCTAGCTTCTCAATCTGAAGGGAAGAAGGAAGTGGCTATAAAGGAGAAGAACCTTGGAGAAGAAGTTCAACCATATGCAGCAGAGTTGGATATCGTACAGAGTGCTTTCAGAAACAATGAGAAGATGATTGAAGACTTGAAGCAGCAACTGGAGTCTGTGCGCTCCGTGGCCCAGGCACAGAAGAGGAAGAGTTTCTGGACTTTGGTGTCATCGGCAACAACAATTATTGCTGCAGCCTCTGTTGCATATGCTGCTAAAGGTCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04530 map04530
Pfam Domains
Protein Families
No domains found.

Protein Analysis

628

Amino Acids

71.39

Weight (kDa)

5.38

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013030)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05130 AT5G27330
fragaria_vesca FvH4_6g04961
malus_domestica MD04G1212300.v1.1 MD12G1227100.v1.1
prunus_persica Prupe.6G332700_v2.0.a1
pyrus_communis pycom04g18840 pycom12g21180
rosa_chinensis RchiOBHm_Chr3g0453461
rosa_laevigata RLG00000025512
rosa_multiflora Rmu_sc0000510.1_g000009
rosa_roxburghii Rroxscaffold_6G00430430
rosa_rugosa Rorug02G0649200
rosa_samantha Rh3AG052000 Rh3BG053500 Rh3CG052800 Rh3DG053600
rosa_wichuraiana Rw3G004010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 190
AasI GACNNNNNNGTC 1 cut(s) 1825
Acc36I ACCTGC 1 cut(s) 190
AccI GTMKAC 1 cut(s) 1174
AccII CGCG 1 cut(s) 125
AciI CCGC 9 cut(s) 99, 102, 105, 108, 353, 707, 743, 941, 1471
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 2 cut(s) 1281, 1656
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 1 cut(s) 1716
AfiI CCNNNNNNNGG 3 cut(s) 189, 544, 643
AflIII ACRYGT 1 cut(s) 123
AgsI TTSAA 9 cut(s) 470, 568, 856, 1024, 1360, 1603, 1688, 1751, 1759
AhdI GACNNNNNGTC 1 cut(s) 127
AhlI ACTAGT 1 cut(s) 41
AjnI CCWGG 1 cut(s) 1791
AjuI GAANNNNNNNTTGG 4 cut(s) 1037, 1069, 1235, 1267
AloI GAACNNNNNNTCC 2 cut(s) 1668, 1700
Alw26I GTCTC 1 cut(s) 173
AlwNI CAGNNNCTG 2 cut(s) 1768, 1859
AoxI GGCC 7 cut(s) 3, 219, 354, 535, 975, 1140, 1788
ApeKI GCWGC 8 cut(s) 196, 938, 1613, 1697, 1762, 1850, 1853, 1871
ArsI GACNNNNNNTTYG 2 cut(s) 293, 325
Asp700I GAANNNNTTC 2 cut(s) 1683, 1811
AspLEI GCGC 1 cut(s) 1782
AspS9I GGNCC 4 cut(s) 220, 355, 1575, 1789
AsuC2I CCSGG 1 cut(s) 494
AsuHPI GGTGA 6 cut(s) 412, 529, 634, 734, 944, 1196
AvaII GGWCC 1 cut(s) 1575
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 1758
BbvCI CCTCAGC 2 cut(s) 1311, 1421
BbvI GCAGC 8 cut(s) 208, 925, 1625, 1709, 1774, 1837, 1858, 1865
BccI CCATC 7 cut(s) 30, 253, 355, 574, 814, 952, 1456
BceAI ACGGC 1 cut(s) 550
BcgI CGANNNNNNTGC 4 cut(s) 521, 555, 1203, 1237
BciT130I CCWGG 1 cut(s) 1793
BcnI CCSGG 1 cut(s) 494
BcoDI GTCTC 1 cut(s) 173
BcuI ACTAGT 1 cut(s) 41
BfaI CTAG 2 cut(s) 42, 1622
BfmI CTRYAG 2 cut(s) 1611, 1851
BfuAI ACCTGC 1 cut(s) 190
BglI GCCNNNNNGGC 1 cut(s) 362
BlpI GCTNAGC 3 cut(s) 488, 1164, 1332
Bme1390I CCNGG 2 cut(s) 494, 1793
Bme18I GGWCC 1 cut(s) 1575
BmeRI GACNNNNNGTC 1 cut(s) 127
BmgT120I GGNCC 4 cut(s) 220, 355, 1575, 1789
BmrFI CCNGG 2 cut(s) 494, 1793
BmsI GCATC 2 cut(s) 1270, 1513
BpiI GAAGAC 1 cut(s) 1758
BpmI CTGGAG 3 cut(s) 566, 938, 1790
Bpu10I CCTNAGC 2 cut(s) 1311, 1421
Bpu1102I GCTNAGC 3 cut(s) 488, 1164, 1332
BpuEI CTTGAG 2 cut(s) 134, 158
BpuMI CCSGG 1 cut(s) 494
BsaHI GRCGYC 1 cut(s) 36
BsaI GGTCTC 1 cut(s) 173
BsaJI CCNNGG 6 cut(s) 114, 637, 978, 1672, 1785, 1791
BsaXI ACNNNNNCTCC 2 cut(s) 1668, 1698
Bsc4I CCNNNNNNNGG 3 cut(s) 189, 544, 643
Bse1I ACTGG 1 cut(s) 1773
Bse3DI GCAATG 2 cut(s) 638, 1231
BseBI CCWGG 1 cut(s) 1793
BseDI CCNNGG 6 cut(s) 114, 637, 978, 1672, 1785, 1791
BseGI GGATG 5 cut(s) 264, 454, 613, 1201, 1504
BseLI CCNNNNNNNGG 3 cut(s) 189, 544, 643
BseMI GCAATG 2 cut(s) 638, 1231
BseNI ACTGG 1 cut(s) 1773
BseRI GAGGAG 7 cut(s) 431, 434, 451, 601, 665, 935, 1145
BseXI GCAGC 8 cut(s) 208, 925, 1625, 1709, 1774, 1837, 1858, 1865
BseYI CCCAGC 1 cut(s) 66
BsgI GTGCAG 1 cut(s) 1526
Bsh1236I CGCG 1 cut(s) 125
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 7 cut(s) 5, 221, 356, 537, 977, 1142, 1790
BsiEI CGRYCG 1 cut(s) 190
BsiSI CCGG 1 cut(s) 493
BslI CCNNNNNNNGG 3 cut(s) 189, 544, 643
BsmAI GTCTC 1 cut(s) 173
BsmI GAATGC 2 cut(s) 1249, 1616
BsnI GGCC 7 cut(s) 5, 221, 356, 537, 977, 1142, 1790
Bso31I GGTCTC 1 cut(s) 173
Bsp143I GATC 1 cut(s) 187
Bsp1720I GCTNAGC 3 cut(s) 488, 1164, 1332
Bsp19I CCATGG 1 cut(s) 114
BspACI CCGC 9 cut(s) 99, 102, 105, 108, 353, 707, 743, 941, 1471
BspANI GGCC 7 cut(s) 5, 221, 356, 537, 977, 1142, 1790
BspFNI CGCG 1 cut(s) 125
BspHI TCATGA 1 cut(s) 1540
BspMAI CTGCAG 2 cut(s) 1615, 1855
BspMI ACCTGC 1 cut(s) 190
BspQI GCTCTTC 1 cut(s) 1505
BspTNI GGTCTC 1 cut(s) 173
BsrDI GCAATG 2 cut(s) 638, 1231
BsrI ACTGG 1 cut(s) 1773
BssECI CCNNGG 6 cut(s) 114, 637, 978, 1672, 1785, 1791
BssMI GATC 1 cut(s) 187
BssNI GRCGYC 1 cut(s) 36
BssT1I CCWWGG 3 cut(s) 114, 978, 1672
Bst2UI CCWGG 1 cut(s) 1793
Bst4CI ACNGT 1 cut(s) 1251
Bst6I CTCTTC 7 cut(s) 290, 323, 398, 1230, 1505, 1796, 1802
BstACI GRCGYC 1 cut(s) 36
BstAPI GCANNNNNTGC 1 cut(s) 1871
BstC8I GCNNGC 1 cut(s) 141
BstDSI CCRYGG 2 cut(s) 114, 1785
BstF5I GGATG 5 cut(s) 264, 454, 613, 1201, 1504
BstFNI CGCG 1 cut(s) 125
BstHHI GCGC 1 cut(s) 1782
BstKTI GATC 1 cut(s) 190
BstMAI GTCTC 1 cut(s) 173
BstMBI GATC 1 cut(s) 187
BstMCI CGRYCG 1 cut(s) 190
BstMWI GCNNNNNNNGC 7 cut(s) 107, 362, 548, 1297, 1622, 1862, 1871
BstNI CCWGG 1 cut(s) 1793
BstSCI CCNGG 2 cut(s) 492, 1791
BstSFI CTRYAG 2 cut(s) 1611, 1851
BstUI CGCG 1 cut(s) 125
BstV1I GCAGC 8 cut(s) 208, 925, 1625, 1709, 1774, 1837, 1858, 1865
BstV2I GAAGAC 1 cut(s) 1758
BsuRI GGCC 7 cut(s) 5, 221, 356, 537, 977, 1142, 1790
BtgI CCRYGG 2 cut(s) 114, 1785
BtgZI GCGATG 2 cut(s) 698, 1449
BtsCI GGATG 5 cut(s) 264, 454, 613, 1201, 1504
BtsI GCAGTG 1 cut(s) 1608
BtsIMutI CAGTG 2 cut(s) 1065, 1608
BveI ACCTGC 1 cut(s) 190
Cac8I GCNNGC 1 cut(s) 141
CaiI CAGNNNCTG 2 cut(s) 1768, 1859
CciI TCATGA 1 cut(s) 1540
CfoI GCGC 1 cut(s) 1782
Cfr13I GGNCC 4 cut(s) 220, 355, 1575, 1789
CseI GACGC 3 cut(s) 44, 114, 131
Csp6I GTAC 1 cut(s) 1715
CviAII CATG 3 cut(s) 115, 274, 1541
CviQI GTAC 1 cut(s) 1715
DpnI GATC 1 cut(s) 189
DpnII GATC 1 cut(s) 187
DrdI GACNNNNNNGTC 1 cut(s) 1825
DriI GACNNNNNGTC 1 cut(s) 127
DseDI GACNNNNNNGTC 1 cut(s) 1825
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 7 cut(s) 290, 323, 398, 1230, 1505, 1796, 1802
Eam1105I GACNNNNNGTC 1 cut(s) 127
EarI CTCTTC 7 cut(s) 290, 323, 398, 1230, 1505, 1796, 1802
EciI GGCGGA 1 cut(s) 722
Eco130I CCWWGG 3 cut(s) 114, 978, 1672
Eco147I AGGCCT 1 cut(s) 977
Eco31I GGTCTC 1 cut(s) 173
Eco32I GATATC 1 cut(s) 1711
Eco47I GGWCC 1 cut(s) 1575
Eco57I CTGAAG 2 cut(s) 1281, 1656
EcoO109I RGGNCCY 1 cut(s) 1575
EcoRII CCWGG 1 cut(s) 1791
EcoRV GATATC 1 cut(s) 1711
EcoT14I CCWWGG 3 cut(s) 114, 978, 1672
ErhI CCWWGG 3 cut(s) 114, 978, 1672
FaeI CATG 3 cut(s) 118, 277, 1544
FatI CATG 3 cut(s) 114, 273, 1540
FauNDI CATATG 2 cut(s) 1693, 1867
FblI GTMKAC 1 cut(s) 1174
FokI GGATG 5 cut(s) 271, 461, 620, 1208, 1511
FspBI CTAG 2 cut(s) 42, 1622
GlaI GCGC 1 cut(s) 1781
GsaI CCCAGC 1 cut(s) 70
GsuI CTGGAG 3 cut(s) 566, 938, 1790
HaeIII GGCC 7 cut(s) 5, 221, 356, 537, 977, 1142, 1790
HapII CCGG 1 cut(s) 493
HgaI GACGC 3 cut(s) 44, 114, 131
HhaI GCGC 1 cut(s) 1782
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 3 cut(s) 118, 277, 1544
Hin6I GCGC 1 cut(s) 1780
HinP1I GCGC 1 cut(s) 1780
HinfI GANTC 6 cut(s) 236, 463, 1095, 1478, 1562, 1772
HpaII CCGG 1 cut(s) 493
HphI GGTGA 6 cut(s) 412, 529, 634, 734, 944, 1196
Hpy166II GTNNAC 1 cut(s) 1175
Hpy188III TCNNGA 7 cut(s) 47, 467, 545, 1148, 1394, 1541, 1816
Hpy8I GTNNAC 1 cut(s) 1175
Hpy99I CGWCG 4 cut(s) 125, 130, 133, 536
HpyCH4III ACNGT 1 cut(s) 1251
HpyCH4V TGCA 8 cut(s) 898, 1224, 1247, 1507, 1613, 1697, 1853, 1865
HpyF10VI GCNNNNNNNGC 7 cut(s) 107, 362, 548, 1297, 1622, 1862, 1871
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 3 cut(s) 118, 277, 1544
HspAI GCGC 1 cut(s) 1780
Kzo9I GATC 1 cut(s) 187
LguI GCTCTTC 1 cut(s) 1505
LmnI GCTCC 3 cut(s) 913, 1087, 1787
Lsp1109I GCAGC 8 cut(s) 208, 925, 1625, 1709, 1774, 1837, 1858, 1865
LweI GCATC 2 cut(s) 1270, 1513
MaeI CTAG 2 cut(s) 42, 1622
MaeIII GTNAC 2 cut(s) 299, 1063
MalI GATC 1 cut(s) 189
MboI GATC 1 cut(s) 187
MfeI CAATTG 2 cut(s) 851, 1406
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 851, 1050, 1073, 1406, 1842
MluI ACGCGT 1 cut(s) 123
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 245, 472, 1781
MmeI TCCRAC 6 cut(s) 111, 303, 708, 813, 844, 1686
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 2 cut(s) 1683, 1811
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 972, 1296, 1554
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 493
MspR9I CCNGG 2 cut(s) 494, 1793
MunI CAATTG 2 cut(s) 851, 1406
Mva1269I GAATGC 2 cut(s) 1249, 1616
MvaI CCWGG 1 cut(s) 1793
MvnI CGCG 1 cut(s) 125
MwoI GCNNNNNNNGC 7 cut(s) 107, 362, 548, 1297, 1622, 1862, 1871
NciI CCSGG 1 cut(s) 494
NcoI CCATGG 1 cut(s) 114
NdeI CATATG 2 cut(s) 1693, 1867
NdeII GATC 1 cut(s) 187
NlaIII CATG 3 cut(s) 118, 277, 1544
NmeAIII GCCGAG 1 cut(s) 662
NmuCI GTSAC 2 cut(s) 299, 1063
PagI TCATGA 1 cut(s) 1540
PaqCI CACCTGC 1 cut(s) 190
PceI AGGCCT 1 cut(s) 977
PciSI GCTCTTC 1 cut(s) 1505
PcsI WCGNNNNNNNCGW 1 cut(s) 345
PctI GAATGC 2 cut(s) 1249, 1616
PdmI GAANNNNTTC 2 cut(s) 1683, 1811
PfeI GAWTC 3 cut(s) 1095, 1478, 1562
Ple19I CGATCG 1 cut(s) 190
PleI GAGTC 3 cut(s) 244, 471, 1780
PpsI GAGTC 3 cut(s) 244, 471, 1780
PpuMI RGGWCCY 1 cut(s) 1575
Psp5II RGGWCCY 1 cut(s) 1575
Psp6I CCWGG 1 cut(s) 1791
PspFI CCCAGC 1 cut(s) 66
PspGI CCWGG 1 cut(s) 1791
PspPI GGNCC 4 cut(s) 220, 355, 1575, 1789
PspPPI RGGWCCY 1 cut(s) 1575
PstI CTGCAG 2 cut(s) 1615, 1855
PstNI CAGNNNCTG 2 cut(s) 1768, 1859
PvuI CGATCG 1 cut(s) 190
RsaI GTAC 1 cut(s) 1716
RsaNI GTAC 1 cut(s) 1715
SapI GCTCTTC 1 cut(s) 1505
SaqAI TTAA 3 cut(s) 972, 1296, 1554
Sau3AI GATC 1 cut(s) 187
Sau96I GGNCC 4 cut(s) 220, 355, 1575, 1789
SchI GAGTC 3 cut(s) 245, 472, 1781
ScrFI CCNGG 2 cut(s) 494, 1793
SfaNI GCATC 2 cut(s) 1270, 1513
SfcI CTRYAG 2 cut(s) 1611, 1851
SinI GGWCC 1 cut(s) 1575
SmlI CTYRAG 2 cut(s) 149, 173
SmoI CTYRAG 2 cut(s) 149, 173
SpeI ACTAGT 1 cut(s) 41
Sse9I AATT 5 cut(s) 851, 1050, 1073, 1406, 1842
SseBI AGGCCT 1 cut(s) 977
SsiI CCGC 9 cut(s) 99, 102, 105, 108, 353, 707, 743, 941, 1471
SspMI CTAG 2 cut(s) 42, 1622
StuI AGGCCT 1 cut(s) 977
StyD4I CCNGG 2 cut(s) 492, 1791
StyI CCWWGG 3 cut(s) 114, 978, 1672
TaaI ACNGT 1 cut(s) 1251
TaqI TCGA 5 cut(s) 186, 318, 531, 1147, 1580
TasI AATT 5 cut(s) 851, 1050, 1073, 1406, 1842
TauI GCSGC 6 cut(s) 104, 107, 110, 356, 944, 1473
TfiI GAWTC 3 cut(s) 1095, 1478, 1562
Tru1I TTAA 3 cut(s) 972, 1296, 1554
Tru9I TTAA 3 cut(s) 972, 1296, 1554
TscAI CASTG 2 cut(s) 1072, 1615
TseFI GTSAC 2 cut(s) 299, 1063
TseI GCWGC 8 cut(s) 196, 938, 1613, 1697, 1762, 1850, 1853, 1871
Tsp45I GTSAC 2 cut(s) 299, 1063
TspDTI ATGAA 3 cut(s) 624, 1529, 1575
TspGWI ACGGA 2 cut(s) 896, 1774
TspRI CASTG 2 cut(s) 1072, 1615
VpaK11BI GGWCC 1 cut(s) 1575
XmiI GTMKAC 1 cut(s) 1174
XmnI GAANNNNTTC 2 cut(s) 1683, 1811
XspI CTAG 2 cut(s) 42, 1622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.