MD13G1002400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0017172
Physical Location & Seq
Forward (+)
148962 .. 149471
510 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1002400.v1.1.491

Sequence Viewer

Length: 510 bp
ATGCTGCAGGCGGCACAGGACGATTTGCTCCTCAAGCTGTCCGTCGACTCTCACATGTCCCACCGCCGTGTTTCTCTGGACGACGATCCCGATATCGACCGTCGCTTTCATGCCCTAAAACTCAAAACTACCAGTGGAGTTGCTGCTGCTGATGCTTCCGCCTCCAATTCCAAATCTAATCGGACCAATTCCGTTCAAGTGGACGAGGAAATAAAGGCAGTCCTCGGCGACGACCTCTCCGCTAGATTCGCCGCTCTCAAGTCTTCATTGCCCACGGCCACGGCCACTCGGTCCTCCTCCTCTGCAACCACCGCCACGTCATCTTACTACTACGATGACTTGGATGATGAGGAGGATGAAGTTGAAAAGTTGATGCGCTGGGCCAAGGACGCCGCACGCCTCGACCCTTCTCCACCTTCCGATTCCGATGACCCGGACAAGTCTGAATCTGACGACTCCGACGACGACAAGAAGAGAAAGGGGAATAGAATAGGGCCCAATTGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

170

Amino Acids

18.44

Weight (kDa)

4.67

Isoelectric Point (pI)

45.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017172)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24370
fragaria_vesca FvH4_4g37240
malus_domestica MD13G1002400.v1.1
prunus_persica Prupe.1G350000_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0447031
rosa_laevigata RLG00000005611
rosa_multiflora Rmu_sc0014071.1_g000008 Rmu_sc0019356.1_g000003
rosa_roxburghii Rroxscaffold_5G00387350
rosa_rugosa Rorug04G0377200
rosa_samantha Rh4BG447700 Rh4CG464800
rosa_wichuraiana Rw4G037520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 254
AccI GTMKAC 1 cut(s) 45
AciI CCGC 7 cut(s) 11, 64, 159, 240, 252, 312, 393
AclWI GGATC 1 cut(s) 80
AcoI YGGCCR 2 cut(s) 276, 282
AcyI GRCGYC 1 cut(s) 390
AflIII ACRYGT 1 cut(s) 54
AgsI TTSAA 2 cut(s) 197, 365
AjiI CACGTC 1 cut(s) 318
AleI CACNNNNGTG 1 cut(s) 66
AluBI AGCT 1 cut(s) 37
AluI AGCT 1 cut(s) 37
AlwI GGATC 1 cut(s) 80
AoxI GGCC 4 cut(s) 276, 282, 381, 494
ApaI GGGCCC 1 cut(s) 498
ApeKI GCWGC 3 cut(s) 4, 143, 146
AspLEI GCGC 1 cut(s) 378
AspS9I GGNCC 5 cut(s) 183, 291, 381, 494, 495
AsuC2I CCSGG 1 cut(s) 434
AvaII GGWCC 2 cut(s) 183, 291
BaeGI GKGCMC 1 cut(s) 498
BanII GRGCYC 1 cut(s) 498
BbsI GAAGAC 1 cut(s) 255
BbvI GCAGC 2 cut(s) 130, 133
BceAI ACGGC 3 cut(s) 51, 291, 297
BcnI CCSGG 1 cut(s) 434
BfaI CTAG 1 cut(s) 243
BfmI CTRYAG 1 cut(s) 5
BisI GCNGC 6 cut(s) 5, 12, 144, 147, 252, 393
BlsI GCNGC 6 cut(s) 6, 13, 145, 148, 253, 394
Bme1390I CCNGG 1 cut(s) 434
Bme18I GGWCC 2 cut(s) 183, 291
BmgBI CACGTC 1 cut(s) 318
BmgT120I GGNCC 5 cut(s) 183, 291, 381, 494, 495
BmiI GGNNCC 1 cut(s) 496
BmrFI CCNGG 1 cut(s) 434
BmsI GCATC 2 cut(s) 142, 363
BpiI GAAGAC 1 cut(s) 255
BpuEI CTTGAG 2 cut(s) 17, 242
BpuMI CCSGG 1 cut(s) 434
BsaHI GRCGYC 1 cut(s) 390
BsaJI CCNNGG 4 cut(s) 223, 273, 279, 384
BsaXI ACNNNNNCTCC 2 cut(s) 221, 251
Bse1I ACTGG 1 cut(s) 132
Bse3DI GCAATG 1 cut(s) 266
BseDI CCNNGG 4 cut(s) 223, 273, 279, 384
BseGI GGATG 2 cut(s) 349, 361
BseMI GCAATG 1 cut(s) 266
BseNI ACTGG 1 cut(s) 132
BseRI GAGGAG 4 cut(s) 20, 286, 289, 365
BseSI GKGCMC 1 cut(s) 498
BseXI GCAGC 2 cut(s) 130, 133
BseYI CCCAGC 1 cut(s) 378
Bsh1285I CGRYCG 1 cut(s) 100
BshFI GGCC 4 cut(s) 278, 284, 383, 496
BsiEI CGRYCG 1 cut(s) 100
BsiSI CCGG 1 cut(s) 434
BslFI GGGAC 1 cut(s) 43
BsmFI GGGAC 1 cut(s) 43
BsnI GGCC 4 cut(s) 278, 284, 383, 496
Bsp120I GGGCCC 1 cut(s) 494
Bsp1286I GDGCHC 1 cut(s) 498
Bsp143I GATC 1 cut(s) 85
BspACI CCGC 7 cut(s) 11, 64, 159, 240, 252, 312, 393
BspANI GGCC 4 cut(s) 278, 284, 383, 496
BspLI GGNNCC 1 cut(s) 496
BspMAI CTGCAG 1 cut(s) 9
BspPI GGATC 1 cut(s) 80
BsrBI CCGCTC 1 cut(s) 254
BsrDI GCAATG 1 cut(s) 266
BsrI ACTGG 1 cut(s) 132
BssECI CCNNGG 4 cut(s) 223, 273, 279, 384
BssMI GATC 1 cut(s) 85
BssNI GRCGYC 1 cut(s) 390
BssT1I CCWWGG 1 cut(s) 384
Bst4CI ACNGT 1 cut(s) 101
Bst6I CTCTTC 1 cut(s) 467
BstACI GRCGYC 1 cut(s) 390
BstC8I GCNNGC 2 cut(s) 9, 397
BstDSI CCRYGG 2 cut(s) 273, 279
BstF5I GGATG 2 cut(s) 349, 361
BstHHI GCGC 1 cut(s) 378
BstKTI GATC 1 cut(s) 88
BstMBI GATC 1 cut(s) 85
BstMCI CGRYCG 1 cut(s) 100
BstMWI GCNNNNNNNGC 5 cut(s) 34, 152, 248, 311, 389
BstNSI RCATGY 1 cut(s) 58
BstSCI CCNGG 1 cut(s) 432
BstSFI CTRYAG 1 cut(s) 5
BstSLI GKGCMC 1 cut(s) 498
BstV1I GCAGC 2 cut(s) 130, 133
BstV2I GAAGAC 1 cut(s) 255
BsuRI GGCC 4 cut(s) 278, 284, 383, 496
BtgI CCRYGG 2 cut(s) 273, 279
BtrI CACGTC 1 cut(s) 318
BtsCI GGATG 2 cut(s) 349, 361
BtsIMutI CAGTG 1 cut(s) 139
Cac8I GCNNGC 2 cut(s) 9, 397
CfoI GCGC 1 cut(s) 378
Cfr13I GGNCC 5 cut(s) 183, 291, 381, 494, 495
CseI GACGC 1 cut(s) 398
CviAII CATG 2 cut(s) 55, 110
CviJI RGCY 5 cut(s) 37, 278, 284, 383, 496
CviKI_1 RGCY 5 cut(s) 37, 278, 284, 383, 496
DpnI GATC 1 cut(s) 87
DpnII GATC 1 cut(s) 85
EaeI YGGCCR 2 cut(s) 276, 282
Eam1104I CTCTTC 1 cut(s) 467
EarI CTCTTC 1 cut(s) 467
EciI GGCGGA 1 cut(s) 148
Eco130I CCWWGG 1 cut(s) 384
Eco24I GRGCYC 1 cut(s) 498
Eco32I GATATC 1 cut(s) 94
Eco47I GGWCC 2 cut(s) 183, 291
EcoO109I RGGNCCY 1 cut(s) 494
EcoRV GATATC 1 cut(s) 94
EcoT14I CCWWGG 1 cut(s) 384
EcoT38I GRGCYC 1 cut(s) 498
ErhI CCWWGG 1 cut(s) 384
FaeI CATG 2 cut(s) 58, 113
FaiI YATR 2 cut(s) 56, 111
FaqI GGGAC 1 cut(s) 43
FatI CATG 2 cut(s) 54, 109
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 6 cut(s) 5, 12, 144, 147, 252, 393
FokI GGATG 2 cut(s) 356, 368
FriOI GRGCYC 1 cut(s) 498
Fsp4HI GCNGC 6 cut(s) 5, 12, 144, 147, 252, 393
FspBI CTAG 1 cut(s) 243
GlaI GCGC 1 cut(s) 377
GluI GCNGC 6 cut(s) 5, 12, 144, 147, 252, 393
GsaI CCCAGC 1 cut(s) 382
HaeIII GGCC 4 cut(s) 278, 284, 383, 496
HapII CCGG 1 cut(s) 434
HgaI GACGC 1 cut(s) 398
HhaI GCGC 1 cut(s) 378
Hin1I GRCGYC 1 cut(s) 390
Hin1II CATG 2 cut(s) 58, 113
Hin6I GCGC 1 cut(s) 376
HinP1I GCGC 1 cut(s) 376
HincII GTYRAC 1 cut(s) 46
HindII GTYRAC 1 cut(s) 46
HinfI GANTC 5 cut(s) 47, 246, 422, 446, 455
HpaII CCGG 1 cut(s) 434
Hpy166II GTNNAC 2 cut(s) 46, 202
Hpy188I TCNGA 6 cut(s) 183, 421, 427, 445, 451, 460
Hpy188III TCNNGA 2 cut(s) 77, 89
Hpy8I GTNNAC 2 cut(s) 46, 202
Hpy99I CGWCG 6 cut(s) 47, 86, 105, 233, 464, 467
HpyAV CCTTC 2 cut(s) 417, 426
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4IV ACGT 1 cut(s) 317
HpyCH4V TGCA 2 cut(s) 7, 305
HpyF10VI GCNNNNNNNGC 5 cut(s) 34, 152, 248, 311, 389
HpySE526I ACGT 1 cut(s) 317
Hsp92I GRCGYC 1 cut(s) 390
Hsp92II CATG 2 cut(s) 58, 113
HspAI GCGC 1 cut(s) 376
Kzo9I GATC 1 cut(s) 85
LmnI GCTCC 1 cut(s) 33
LpnPI CCDG 5 cut(s) 2, 62, 145, 364, 447
Lsp1109I GCAGC 2 cut(s) 130, 133
LweI GCATC 2 cut(s) 142, 363
MaeI CTAG 1 cut(s) 243
MaeII ACGT 1 cut(s) 317
MalI GATC 1 cut(s) 87
MbiI CCGCTC 1 cut(s) 254
MboI GATC 1 cut(s) 85
MboII GAAGA 2 cut(s) 255, 484
MfeI CAATTG 1 cut(s) 499
MhlI GDGCHC 1 cut(s) 498
MluCI AATT 3 cut(s) 166, 187, 499
MlyI GAGTC 2 cut(s) 41, 449
MmeI TCCRAC 1 cut(s) 483
MslI CAYNNNNRTG 1 cut(s) 66
MspI CCGG 1 cut(s) 434
MspR9I CCNGG 1 cut(s) 434
MunI CAATTG 1 cut(s) 499
MwoI GCNNNNNNNGC 5 cut(s) 34, 152, 248, 311, 389
NciI CCSGG 1 cut(s) 434
NdeII GATC 1 cut(s) 85
NlaIII CATG 2 cut(s) 58, 113
NlaIV GGNNCC 1 cut(s) 496
NmeAIII GCCGAG 1 cut(s) 204
NspI RCATGY 1 cut(s) 58
OliI CACNNNNGTG 1 cut(s) 66
PciI ACATGT 1 cut(s) 54
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 459
PfeI GAWTC 3 cut(s) 246, 422, 446
PkrI GCNGC 6 cut(s) 6, 13, 145, 148, 253, 394
PleI GAGTC 2 cut(s) 41, 449
PpsI GAGTC 2 cut(s) 41, 449
PscI ACATGT 1 cut(s) 54
PspFI CCCAGC 1 cut(s) 378
PspN4I GGNNCC 1 cut(s) 496
PspOMI GGGCCC 1 cut(s) 494
PspPI GGNCC 5 cut(s) 183, 291, 381, 494, 495
PstI CTGCAG 1 cut(s) 9
RseI CAYNNNNRTG 1 cut(s) 66
SalI GTCGAC 1 cut(s) 44
SatI GCNGC 6 cut(s) 5, 12, 144, 147, 252, 393
Sau3AI GATC 1 cut(s) 85
Sau96I GGNCC 5 cut(s) 183, 291, 381, 494, 495
SchI GAGTC 2 cut(s) 41, 449
ScrFI CCNGG 1 cut(s) 434
SduI GDGCHC 1 cut(s) 498
SetI ASST 4 cut(s) 39, 237, 320, 418
SfaNI GCATC 2 cut(s) 142, 363
SfcI CTRYAG 1 cut(s) 5
SinI GGWCC 2 cut(s) 183, 291
SmiMI CAYNNNNRTG 1 cut(s) 66
SmlI CTYRAG 2 cut(s) 32, 257
SmoI CTYRAG 2 cut(s) 32, 257
Sse9I AATT 3 cut(s) 166, 187, 499
SsiI CCGC 7 cut(s) 11, 64, 159, 240, 252, 312, 393
SspMI CTAG 1 cut(s) 243
StyD4I CCNGG 1 cut(s) 432
StyI CCWWGG 1 cut(s) 384
TaaI ACNGT 1 cut(s) 101
TaiI ACGT 1 cut(s) 320
TaqI TCGA 3 cut(s) 45, 96, 402
TaqII GACCGA 1 cut(s) 279
TasI AATT 3 cut(s) 166, 187, 499
TauI GCSGC 3 cut(s) 14, 254, 395
TfiI GAWTC 3 cut(s) 246, 422, 446
TscAI CASTG 1 cut(s) 139
TseI GCWGC 3 cut(s) 4, 143, 146
TspDTI ATGAA 3 cut(s) 98, 255, 372
TspGWI ACGGA 2 cut(s) 31, 181
TspRI CASTG 1 cut(s) 139
VpaK11BI GGWCC 2 cut(s) 183, 291
XceI RCATGY 1 cut(s) 58
XmiI GTMKAC 1 cut(s) 45
XspI CTAG 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.